CN112239380A - Amino acid biological preparation for improving stress resistance of crops - Google Patents
Amino acid biological preparation for improving stress resistance of crops Download PDFInfo
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- CN112239380A CN112239380A CN202011042810.5A CN202011042810A CN112239380A CN 112239380 A CN112239380 A CN 112239380A CN 202011042810 A CN202011042810 A CN 202011042810A CN 112239380 A CN112239380 A CN 112239380A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
- C05F5/002—Solid waste from mechanical processing of material, e.g. seed coats, olive pits, almond shells, fruit residue, rice hulls
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention relates to an amino acid biological preparation for improving stress resistance of crops, which comprises the following components in parts by weight: 5-12 parts of microbial preparation, 15-30 parts of paper mulberry powder, 15-25 parts of suaeda salsa powder, 30-70 parts of soybean meal and 15-25 parts of bagasse; the microbial preparation comprises the following components: bacillus amyloliquefaciens, azotobacter, yeast, Bacillus natto and Bacillus laterosporus are mixed according to the volume ratio of 6-15:2-4:2-5:2-5:3-7, wherein the bacterial activity of each bacterium is not less than 109Per gram. The invention provides a large amount of amino acid for crops by utilizing various materials, creates a water-retaining, fertilizer-retaining and healthy growth environment for the growth of the crops, and improves the stress resistance of the crops.
Description
Technical Field
The invention relates to a biological preparation, in particular to an amino acid biological preparation for improving stress resistance of crops.
Background
China is a big agricultural country, although the farmers are vast, the arable area is small, the poor land is large, some soil is lack of fertilizer and water, people apply chemical fertilizers in large quantities for a long time to maintain the growth of crops and the formation of yield in order to pursue the yield of the crops, the stress resistance of the crops is continuously reduced, various nutrients in the soil are seriously damaged, the concentration of acidic and toxic substances in the soil is rapidly increased, the soil is generally hardened and acidified, the quality of agricultural products is remarkably reduced, even the root systems of the crops are injured, even if a large amount of fertilizers are put into the soil, the crops are difficult to absorb and utilize, the plants cannot utilize the nutrient substances, and the growth and development of the crops are inhibited.
Disclosure of Invention
Aiming at the existing defects, the invention provides the amino acid biological agent for improving the stress resistance of crops.
The technical scheme adopted by the invention for solving the technical problems is as follows: an amino acid biological preparation for improving stress resistance of crops comprises the following components in parts by weight: 5-12 parts of microbial preparation, 15-30 parts of paper mulberry powder, 15-25 parts of suaeda salsa powder, 30-70 parts of soybean meal and 15-25 parts of bagasse; the microbial preparation comprises the following components: bacillus amyloliquefaciens, azotobacter, yeast, Bacillus natto and Bacillus laterosporus are mixed according to the volume ratio of 6-15:2-4:2-5:2-5:3-7, wherein the bacterial activity of each bacterium is not less than 109Per gram.
Preferably, the tea-seed cake further comprises one or more of tea bran powder, ailanthus altissima powder and kelp residue, and the weight portion of the tea bran powder, the ailanthus altissima powder and the kelp residue is 10-15 portions.
Preferably, the microbial preparation further comprises any one or more of 2-4 volumes of actinomycetes, 1-3 volumes of bacillus megaterium and 1-3 volumes of bacillus subtilis.
Preferably, the material also comprises 4-8 parts by weight of mineral materials, and the mineral materials comprise one or more of bentonite, zeolite and shell powder.
Preferably, the preservative also comprises 10-20 parts of potassium fulvate and 2-5 parts of chitosan by weight.
Preferably, the biological agent is prepared by the following method:
s1, weighing and uniformly mixing 15-30 parts by weight of dry paper mulberry powder, 15-25 parts by weight of suaeda salsa powder, 30-70 parts by weight of soybean meal and 15-25 parts by weight of bagasse to obtain a basic mixture;
s2, preparing a microbial preparation, namely mixing bacillus amyloliquefaciens, azotobacter, saccharomycetes, bacillus natto and bacillus laterosporus according to a volume ratio of 6-15:2-4:2-5:2-5:3-7 to prepare a mixed bacterial liquid, wherein the bacterial activity of each bacterium is not less than 109Per gram;
s3, transferring the basic mixture prepared in the S1 into a fermentation tank, adding the mixed bacterial liquid prepared in the S2 into the fermentation tank, stirring at the speed of 200rpm, adding deionized water to control the water content to be 40-48 wt%, stirring and fermenting at the temperature of 30-35 ℃ for 3-5 days, and cooling to room temperature to package a finished product.
Preferably, the particle sizes of the paper mulberry powder, the suaeda salsa powder, the soybean meal powder and the bagasse are all 25-60 meshes.
The invention has the beneficial effects that: the bacillus amyloliquefaciens can generate a plurality of active microorganisms, enzymes, organic acids and antibiotic substances, and the substances can effectively improve the micro-ecosystem of soil; the nano-bean sprout bacillus can effectively decompose plant fiber and phosphate fertilizer and provide a carbon source; the soybean meal is rich in various nutrient substances such as lactic acid, vitamins, amino acids and unknown growth promoting factors; the paper mulberry is rich in protein, amino acid and vitamin; suaeda salsa is rich in protein and vitamins; the yeast is beneficial to the fermentation of microorganisms; through the interaction between the nutrient components and beneficial flora of the soil, the nutrient content of the soil is increased, the soil fertility is improved, and the stress resistance of crops is further improved; meanwhile, the suaeda salsa is rich in potassium salt, has an active repairing effect on soil, and reduces the content of heavy metal cadmium in the soil; the nitrogen-fixing bacteria are beneficial to the reinforcement of nitrogen, and the bacillus laterosporus promotes the growth of plants; bagasse is a vascular bundle of sugarcane, has longer fibers, can effectively reduce the pH value of soil and avoid caking of the soil, can preserve moisture and provide nutrition for the soil through the fibers, is beneficial to the conduction of moisture, inorganic salt and organic substances, can effectively maintain the moisture and the nutrition of a plant growth environment, and meanwhile, fiber tissues can be decomposed through bacillus natto; thus forming different microbial forms in the soil, maintaining good micro-ecology of the soil and improving the stress resistance of crops.
Detailed Description
To more clearly illustrate the objects, technical solutions and advantages of the embodiments of the present invention, the present invention will be further described in conjunction with the following embodiments for clear and complete description, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present invention without inventive step, are within the scope of the present invention. Meanwhile, the instruments, reagents, materials and the like which are referred to in the invention are all conventional instruments, reagents, materials and the like in the prior art and can be obtained through normal commercial routes unless otherwise specified. The experimental methods, detection methods, and the like mentioned in the examples are conventional experimental methods, detection methods, and the like in the prior art unless otherwise specified.
The embodiment of the invention provides an amino acid biological agent for improving stress resistance of crops, which comprises the following components in parts by weight: 5-12 parts of microbial preparation, 15-30 parts of paper mulberry powder, 15-25 parts of suaeda salsa powder, 30-70 parts of soybean meal and 15-25 parts of bagasse; the microbial preparation comprises the following components: bacillus amyloliquefaciens, azotobacter, yeast, Bacillus natto and Bacillus laterosporus are mixed according to the volume ratio of 6-15:2-4:2-5:2-5:3-7, wherein the bacterial activity of each bacterium is not less than 109Per gram; pulverizing the above powders with corresponding pulverizer, and culturing various bacteria with existing culture method; the particle sizes of the paper mulberry powder, the suaeda salsa powder, the soybean meal powder and the bagasse are all 25-60 meshes, so that the paper mulberry powder, the suaeda salsa powder, the soybean meal powder and the bagasse have relatively large surface areas, and are favorable for uniform mixing and effective utilization of various bacteria in microbial preparations. The paper mulberry powder can be prepared from paper mulberry leaf, paper mulberry bark, paper mulberry root or paper mulberry seed; the Suaeda salsa powder is prepared from Suaeda salsa seeds. The biological preparation is prepared by the following method:
s1, weighing and uniformly mixing 15-30 parts by weight of dry paper mulberry powder, 15-25 parts by weight of suaeda salsa powder, 30-70 parts by weight of soybean meal and 15-25 parts by weight of bagasse to obtain a basic mixture;
s2, preparing a microbial preparation, namely mixing bacillus amyloliquefaciens, azotobacter, saccharomycetes, bacillus natto and bacillus laterosporus according to a volume ratio of 6-15:2-4:2-5:2-5:3-7 to prepare a mixed bacterial liquid, wherein the bacterial activity of each bacterium is not less than 109Per gram;
s3, transferring the basic mixture prepared in the S1 into a fermentation tank, adding the mixed bacterial liquid prepared in the S2 into the fermentation tank, stirring at the speed of 200rpm, adding deionized water to control the water content to be 40-48 wt%, stirring and fermenting at the temperature of 30-35 ℃ for 3-5 days, and cooling to room temperature to package a finished product.
Example 1
(1) Weighing 25 parts of dry broussonetia papyrifera powder with the granularity of 25-60 meshes, 18 parts of suaeda salsa powder, 40 parts of soybean meal and 20 parts of bagasse in parts by weight, and mixing the materials to prepare a mixture 1;
(2) the viable amount of the bacteria is not less than 109Mixing bacillus amyloliquefaciens, azotobacter, saccharomycetes, natto bacillus and bacillus laterosporus according to the volume ratio of 10:2.5:4:3:4 to prepare a mixture 2;
(3) transferring the mixture 1 into a fermentation tank, adding the mixture 2, fermenting at 32 deg.C under stirring at 200rpm for 3 days while adding deionized water to control water content at 45 wt%, and cooling to room temperature.
Example 2
(1) Weighing 30 parts of dry broussonetia papyrifera powder with the granularity of 25-60 meshes, 20 parts of suaeda salsa powder, 50 parts of soybean meal and 15 parts of bagasse in parts by weight, and mixing the materials to prepare a mixture 1;
(2) the viable amount of the bacteria is not less than 109Mixing bacillus amyloliquefaciens, azotobacter, saccharomycetes, natto bacillus and bacillus laterosporus according to the volume ratio of 14:2:4:2:5 to prepare a mixture 2;
(3) transferring the mixture 1 into a fermentation tank, adding the mixture 2, fermenting at 32 deg.C under stirring at 200rpm for 3 days while adding deionized water to control water content at 45 wt%, and cooling to room temperature.
Further improvement, the biological preparation also comprises one or more of tea bran powder, ailanthus altissima powder and kelp residue, the weight portion of the tea bran powder is 10-15, the tea bran powder can effectively inhibit the growth of pathogenic bacteria and can be automatically decomposed; the ailanthus powder also has long fibers, so that the soil is prevented from caking, and simultaneously, the moisture and the nutrients are favorably kept; the kelp residue contains rich crude fiber, and also contains mineral elements such as iodine, potassium, calcium, manganese, iron, zinc and the like and protein, and can be used as an adsorbent to adsorb microorganisms and promote the reaction of the microorganisms.
Example 3
(2) Weighing 25 parts of dry broussonetia papyrifera powder with the granularity of 25-60 meshes, 18 parts of suaeda salsa powder, 40 parts of soybean meal, 20 parts of bagasse, 7 parts of ailanthus powder and 5 parts of tea bran powder in parts by weight, and mixing the materials to prepare a mixture 1;
(2) the viable amount of the bacteria is not less than 109Mixing bacillus amyloliquefaciens, azotobacter, saccharomycetes, natto bacillus and bacillus laterosporus according to the volume ratio of 10:3:4:3:4 to prepare a mixture 2;
(3) transferring the mixture 1 into a fermentation tank, adding the mixture 2, fermenting at 32 deg.C under stirring at 200rpm for 3 days while adding deionized water to control water content at 40 wt%, and cooling to room temperature.
In a further improvement, the microbial preparation also comprises any one or more of 2-4 volumes of actinomycetes, 1-3 volumes of bacillus megaterium and 1-3 volumes of bacillus subtilis, and the competition relationship between the bacillus megaterium and the bacillus subtilis is utilized to accelerate the metabolism, and the synergistic effect between the bacillus megaterium and the bacillus subtilis, the growth of crops is further promoted, and the fertility of soil is increased.
Example 4
(1) Weighing 25 parts of dry broussonetia papyrifera powder with the granularity of 25-60 meshes, 18 parts of suaeda salsa powder, 40 parts of soybean meal and 20 parts of bagasse in parts by weight, and mixing the materials to prepare a mixture 1;
(2) the viable amount of the bacteria is not less than 109Mixing bacillus amyloliquefaciens, azotobacter, saccharomycetes, natto bacillus, bacillus laterosporus, actinomycetes, bacillus megaterium and bacillus subtilis according to the volume ratio of 10:2.5:4:3:4:2:2:2.5 to prepare a mixture 2;
(3) transferring the mixture 1 into a fermentation tank, adding the mixture 2, fermenting at 32 deg.C under stirring at 200rpm for 3 days while adding deionized water to control water content at 45 wt%, and cooling to room temperature.
The further improvement comprises 4-8 parts by weight of mineral materials, wherein the mineral materials comprise one or more of bentonite, zeolite and shell powder, and the adsorption capacity of the product can be enhanced by utilizing the structural characteristics of the mineral materials, so that the long-term effective reaction of microorganisms can be kept.
Example 5
(1) Weighing 25 parts of dry broussonetia papyrifera powder with the granularity of 25-60 meshes, 18 parts of suaeda salsa powder, 40 parts of soybean meal, 20 parts of bagasse, 4 parts of zeolite and 3 parts of shell powder in parts by weight, and mixing the materials to prepare a mixture 1;
(2) the viable amount of the bacteria is not less than 109Mixing bacillus amyloliquefaciens, azotobacter, saccharomycetes, natto bacillus and bacillus laterosporus according to the volume ratio of 10:2.5:4:3:4 to prepare a mixture 2;
(3) transferring the mixture 1 into a fermentation tank, adding the mixture 2, fermenting at 32 deg.C under stirring at 200rpm for 3 days while adding deionized water to control water content at 48 wt%, and cooling to room temperature.
The improved fertilizer also comprises 10-20 parts of potassium fulvate and 2-5 parts of chitosan, wherein the potassium fulvate can chelate macro-and micronutrients to ensure that the macronutrient and the micronutrient are better utilized by plants, prevent and treat plant diseases, enhance waterlogging resistance, stimulate microscopic biological activity of the plants, improve nutrient absorption, promote germination and growth of the plants, accelerate precipitation and decomposition and improve soil structure; the reaction activity of amino groups in the molecular structure of the chitosan is stronger, so that the chitosan has biodegradability, biocompatibility, good adsorbability, film-forming property, permeability, fiber-forming property, hygroscopicity and moisture retention, and provides moisture and nutrients for survival of microorganisms. Thus, the crop stress resistance and disease resistance are further improved, harmful bacteria can be killed, insects can be killed, various acids can be secreted, the crop resistance is enhanced, the soil fertility is enhanced, the crop growth capability is stimulated, the root growth is promoted, the root absorption surface is enlarged, the absorption capability of elements outside the primary root hair absorption range is enhanced, and the crop vitality is improved.
Example 6
(1) Weighing 25 parts of dry broussonetia papyrifera powder with the granularity of 25-60 meshes, 18 parts of suaeda salsa powder, 40 parts of soybean meal and 20 parts of bagasse in parts by weight, and mixing the materials to prepare a mixture 1;
(2) the viable amount of the bacteria is not less than 109Mixing bacillus amyloliquefaciens, azotobacter, saccharomycetes, natto bacillus and bacillus laterosporus according to the volume ratio of 10:2.5:4:3:4 to prepare a mixture 2;
(3) transferring the mixture 1 to a fermentation tank, adding the mixture 2, 18 parts by weight of potassium fulvate and 3 parts by weight of chitosan, stirring and fermenting at 32 ℃ at a speed of 200rpm for 3 days, adding deionized water to control the water content to be 45 wt%, and cooling to room temperature for packaging.
Finally, the products in the above embodiments are spread on the land to be improved and vegetables are planted, compared with the conventionally planted vegetables, the planting area of each vegetable is 15m2180 plants are planted, and the test results are as follows:
TABLE 1 Effect of different treatments on the growth and development of the vegetables (after 60 days)
TABLE 1
The biological agent effectively enhances the soil fertility in a weak soil environment, stimulates the growth of crops, enables the plants to quickly and effectively obtain nutrients and moisture for a long time and healthily develop and grow, and improves the stress resistance of the crops.
It will be understood that modifications and variations can be made by persons skilled in the art in light of the above teachings and all such modifications and variations are intended to be included within the scope of the invention as defined in the appended claims.
Claims (7)
1. Amino acid biological preparation for improving stress resistance of crops and application thereofIs characterized in that: the composition comprises the following components in parts by weight: 5-12 parts of microbial preparation, 15-30 parts of paper mulberry powder, 15-25 parts of suaeda salsa powder, 30-70 parts of soybean meal and 15-25 parts of bagasse; the microbial preparation comprises the following components: bacillus amyloliquefaciens, azotobacter, yeast, Bacillus natto and Bacillus laterosporus are mixed according to the volume ratio of 6-15:2-4:2-5:2-5:3-7, wherein the bacterial activity of each bacterium is not less than 109Per gram.
2. The amino acid biological preparation for improving crop stress resistance according to claim 1, which is characterized in that: also comprises one or more of tea bran powder, ailanthus altissima powder and kelp residue, and the weight portion of the tea bran powder, the ailanthus altissima powder and the kelp residue is 10-15 portions.
3. The amino acid biological preparation for improving crop stress resistance according to claim 1, which is characterized in that: the microbial preparation also comprises any one or more of 2-4 volumes of actinomycetes, 1-3 volumes of bacillus megaterium and 1-3 volumes of bacillus subtilis.
4. The amino acid biological preparation for improving crop stress resistance according to claim 1, which is characterized in that: the material also comprises 4-8 parts by weight of mineral materials, wherein the mineral materials comprise one or more of bentonite, zeolite and shell powder.
5. The amino acid biological preparation for improving crop stress resistance according to claim 1, which is characterized in that: also comprises 10 to 20 weight portions of potassium fulvate and 2 to 5 weight portions of chitosan.
6. The amino acid biological preparation for improving crop stress resistance according to claim 1, which is characterized in that: the preparation method comprises the following steps:
s1, weighing and uniformly mixing 15-30 parts by weight of dry paper mulberry powder, 15-25 parts by weight of suaeda salsa powder, 30-70 parts by weight of soybean meal and 15-25 parts by weight of bagasse to obtain a basic mixture;
s2 preparation of microbial preparation by sporulation of amyloliquefaciensBacillus, azotobacter, microzyme, bacillus natto and bacillus laterosporus are mixed according to the volume ratio of 6-15:2-4:2-5:2-5:3-7 to prepare mixed bacterial liquid, wherein the bacterial activity of each bacterium is not less than 109Per gram;
s3, transferring the basic mixture prepared in the S1 into a fermentation tank, adding the mixed bacterial liquid prepared in the S2 into the fermentation tank, stirring at the speed of 200rpm, adding deionized water to control the water content to be 40-48 wt%, stirring and fermenting at the temperature of 30-35 ℃ for 3-5 days, and cooling to room temperature to package a finished product.
7. The amino acid biological preparation for improving crop stress resistance according to claim 1, which is characterized in that: the particle sizes of the broussonetia papyrifera powder, the suaeda salsa powder, the soybean meal powder and the bagasse are all 25-60 meshes.
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| CN113912461A (en) * | 2021-11-18 | 2022-01-11 | 海南大学 | Foliar fertilizer for resisting plant leaf fungal diseases |
| CN113912450A (en) * | 2021-11-18 | 2022-01-11 | 海南大学 | Composite foliar fertilizer capable of preventing and treating plant diseases |
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Cited By (2)
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| CN113912461A (en) * | 2021-11-18 | 2022-01-11 | 海南大学 | Foliar fertilizer for resisting plant leaf fungal diseases |
| CN113912450A (en) * | 2021-11-18 | 2022-01-11 | 海南大学 | Composite foliar fertilizer capable of preventing and treating plant diseases |
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Application publication date: 20210119 |