CN111548975A - Enzyme microorganism seed dressing agent and preparation and use methods thereof - Google Patents
Enzyme microorganism seed dressing agent and preparation and use methods thereof Download PDFInfo
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- CN111548975A CN111548975A CN202010553684.3A CN202010553684A CN111548975A CN 111548975 A CN111548975 A CN 111548975A CN 202010553684 A CN202010553684 A CN 202010553684A CN 111548975 A CN111548975 A CN 111548975A
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- C12N1/20—Bacteria; Culture media therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The invention relates to an enzyme microorganism seed dressing agent and a preparation method and a use method thereof. The seed dressing agent consists of natural organic materials of rice bran, wheat bran, rice hull powder, sawdust, fishbone powder, shale, zeolite, turf, brown sugar and a ferment microorganism composite microbial agent, is prepared by material treatment, aerobic fermentation, crushing and sieving and qualification detection, and is directly mixed with tuber crops according to a certain proportion for seed dressing, wherein the viable bacteria amount in a ferment composite bacterial strain is more than 10 hundred million/g, and the seed dressing agent comprises at least one of bacteria, fungi and actinomycetes in seven beneficial bacteria of bacillus subtilis, saccharomyces cerevisiae, lactobacillus plantarum, streptomyces, aspergillus oryzae, trichoderma viride and fomes fomentarius, wherein the ratio of the bacteria, the fungi and the actinomycetes is 40-60%: 20-30%: 20 to 30 percent. The invention has the advantages of natural and organic raw materials, rich nutrient substances, simple manufacturing process, convenient use, large viable count, no seed burning, high germination rate, regular budding, robust emergence of seedlings, less diseases, yield increase, quality improvement and the like.
Description
Technical Field
The invention belongs to the technical field of agricultural enzyme bacteria, and particularly relates to an enzyme microorganism seed dressing agent and a preparation method and a use method thereof.
Background
The problem of "three farmers" has become the primary problem in national economy of China, and draws attention of all parties in China. The advanced scientific technology at home and abroad is adopted to transform the traditional agriculture, the pace of the modern agriculture is accelerated, and agricultural and sideline products with high quality and high added value, which are demanded by markets at home and abroad, are produced, so that the method is an important measure for solving the problem of 'three farmers'. The ferment bacteria technology is regarded as the evolution type biological technology crystal, is known as the future hope of Chinese agriculture, and has great significance and wide prospect for solving the problems of three kinds of agriculture.
The seed dressing agent is a substance which can be mixed with seeds, tubers or rhizomes, is used for improving the seedling emergence condition of the seeds, the growth condition of seedlings, has the effects of preventing and treating diseases and insect pests, has the effects of promoting beneficial microorganisms in soil, improving the saline-alkali property and drought of the soil, has the function of promoting the strengthening of crops and finally achieves the purposes of increasing the yield and improving the quality. However, the existing seed dressing agent has the following disadvantages:
1. the pesticide is prepared by processing pesticide raw materials, hormone and seed manure, has high toxicity, is easy to residue and enrich in soil and crops, pollutes the environment and damages the health of human bodies.
2. The seed dressing agent has strong pertinence, one seed dressing agent can only be suitable for one crop, and the use is not wide.
3. The seed dressing treatment is carried out by adopting soil methods such as plant ash, fluffy soil loosening and the like, and the disease prevention effect is poor.
4. Some biological seed dressings, but because the preparation process requirement is high, the operation is tedious, the strain is single or the disease prevention effect can not be widely applied generally.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention provides an enzyme microorganism seed dressing agent and a preparation method and a use method thereof by utilizing an enzyme bacterium technology. The preparation process is simple to operate, has low cost, is rich in various beneficial microorganisms, auxin, anti-virus enzyme and active polypeptide, and is an enzyme microorganism seed dressing agent which integrates the functions of quickly healing wounds, promoting germination, inhibiting pathogenic bacteria invasion and protecting environment and has long effect.
The technical scheme adopted by the invention for solving the technical problems is as follows:
1. the invention provides an enzyme microorganism seed dressing agent which is prepared from the following raw materials in parts by weight:
0.05-0.15 part of ferment compound strain, 1-3 parts of rice bran or wheat bran, 3-5 parts of rice hull powder, 0.5-1.5 parts of saw dust, 0.5-1.5 parts of fishbone powder, 5-7 parts of shale, 1-3 parts of zeolite, 2-5 parts of grass peat and 0.05-0.15 part of brown sugar;
the ferment composite bacteria strain has live bacteria amount more than 10 hundred million/g, and comprises at least one of bacteria, fungi and actinomycetes in seven beneficial bacteria of bacillus subtilis, saccharomyces cerevisiae, lactobacillus plantarum, streptomyces, aspergillus oryzae, trichoderma viride and fomes fomentarius, wherein the ratio of the bacteria, the fungi and the actinomycetes is 40-60%: 20-30%: 20 to 30 percent.
The reason for selecting the raw materials is as follows:
1) the materials are beneficial and harmless to soil, environment and later-period crops, organic materials such as rice bran, wheat bran, rice hulls and the like provide soil components and organic matters and provide crop growth nutrition, soil is loosened such as shale and zeolite and the like to provide trace elements required by crops, seedlings and roots are strengthened and the like, and the brown sugar also has the effect of promoting wound healing;
2) the specific mixture ratio needs to meet the carbon-nitrogen ratio required by the growth of microorganisms, and the carbon-nitrogen ratio is kept at 20-25;
3) the carbon source and the nitrogen source are composite materials, and rice bran, wheat bran, rice hulls, brown sugar, fishbone meal and the like with high bacteria number in earlier stage culture of the strain are selected as the composite carbon source and nitrogen source;
4) minerals such as shale, zeolite, turf and the like are added to provide trace elements required by bacterial growth, and trace element micromolecules required by crop growth are provided after bacterial catabolism.
Preferably, the feed consists of the following raw materials in parts by weight:
0.10 part of ferment compound strain, 2 parts of rice bran or wheat bran, 4 parts of rice hull powder, 1.0 part of saw dust, 1.0 part of fishbone powder, 6 parts of shale, 2 parts of zeolite, 4 parts of grass carbon and 0.10 part of brown sugar;
the ferment composite bacteria strain has live bacteria amount more than 10 hundred million/g, contains seven beneficial bacteria of bacillus subtilis, saccharomyces cerevisiae, lactobacillus plantarum, streptomyces, aspergillus oryzae, trichoderma viride and fomes fomentarius, and has the proportion of 25 percent according to the bacillus subtilis, saccharomycetes, lactobacillus, actinomycetes and filamentous fungi: 25%: 20%: 15%: 15 percent.
2. The invention also provides a preparation method of the enzyme microorganism seed dressing agent, which comprises the following specific steps:
1) preparation of fermentation materials: mixing the raw materials as recited in claim 1, wherein the ratio of carbon to nitrogen in the mixture is controlled to be 25: 1-40: 1;
2) aerobic fermentation: adding water to control the water content of the mixture to be about 55%, uniformly mixing, performing aerobic fermentation, turning the pile for the first time when the temperature in the pile reaches 50 ℃, turning the pile for every 7-10 days, and stopping fermentation until the temperature is reduced to about 30 ℃ and tends to be stable;
3) crushing and sieving: reducing the water content of the material fermented in the step 2) to below 20%, and then carrying out crushing and sieving treatment to obtain a semi-finished product of the enzyme microorganism seed dressing agent;
4) and (4) qualified detection: and (3) detecting the seed dressing agent prepared in the step 3), wherein the qualified product is judged as the active beneficial bacteria of more than or equal to 2.0 hundred million/g, and the qualified seed dressing agent is subpackaged and warehoused.
3. The invention also provides a use method of the enzyme microorganism seed dressing agent, which comprises the following steps of mixing the prepared seed dressing agent with seed potatoes or ginger blocks according to a ratio of 1: mixing at a ratio of 25-37, and placing in earthen bed for germination.
Optionally, if a turnover basket is needed, a big bag is placed in the turnover basket for heat preservation and moisture preservation, the big bag is perforated to keep ventilation, the mixed seed potato blocks or ginger blocks and sandy soil are stacked in the turnover basket according to a layer of sandy soil and a layer of potato blocks or ginger blocks, and the ratio of the sandy soil to the seed potato blocks or the ginger blocks is 1: and 3, placing the mixture in an environment with the temperature of 15-20 ℃ for accelerating germination.
Compared with the prior art, the enzyme microorganism seed dressing agent and the preparation and use methods thereof have the following beneficial effects:
the invention can protect crops from seed burning in the early stage of planting, has high germination rate, regular germination, robust seedling emergence and less exhaust diseases; the beneficial live bacteria in the invention can protect the whole growth period of crops in the middle and later period of planting, promote the thickening of leaves, improve photosynthesis, increase yield, resist premature senility and improve the quality of crops. In addition, the invention has the advantages of simple operation, time and labor saving, health and environmental protection, good economic benefit and social benefit, and is beneficial to large-area popularization and use.
Drawings
FIG. 1 is a root growth diagram of a potato (from left to right, without use, with use of chemistry, with use of example one) according to the present invention;
FIG. 2 shows the sprouting of potatoes after the use of the example.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example one
The invention relates to a ferment microorganism seed dressing agent, which consists of the following raw materials in parts by weight:
0.05 part of ferment compound strain, 3 parts of rice bran or wheat bran, 3 parts of rice hull powder, 0.5 part of saw dust, 1.5 parts of fishbone powder, 5 parts of shale, 3 parts of zeolite, 2 parts of grass carbon and 0.15 part of brown sugar.
The viable bacteria amount in the ferment composite bacteria strain is more than 10 hundred million/g, and the ferment composite bacteria strain comprises bacillus subtilis, saccharomyces cerevisiae, lactobacillus plantarum, streptomyces and trichoderma viride, and the mixture ratio is 25%: 25%: 20%: 15%: 15 percent.
At least one of bacteria, fungi and actinomycetes, wherein the ratio of bacteria, fungi and actinomycetes is 40-60%: 20-30%: 20 to 30 percent.
The ferment composite bacteria strain has live bacteria amount more than 10 hundred million/g, contains seven beneficial bacteria of bacillus subtilis, saccharomyces cerevisiae, lactobacillus plantarum, streptomyces, aspergillus oryzae, trichoderma viride and fomes fomentarius, and has the proportion of bacteria, saccharomycetes, lactobacillus, actinomycetes and filamentous fungi of 25 percent: 25%: 20%: 15%: 15 percent.
A preparation method of an enzyme microorganism seed dressing agent comprises the following specific steps:
1) preparation of fermentation materials: the raw materials described in 1 above were prepared, and the carbon-nitrogen ratio was controlled at 25: 1-40: 1.
2) Aerobic fermentation: adding water to control the water content of the mixture to be about 55%, uniformly mixing, performing aerobic fermentation, turning the pile for the first time when the temperature in the pile reaches 50 ℃, turning the pile for every 7-10 days, and stopping fermentation until the temperature is reduced to about 30 ℃ and tends to be stable.
3) Crushing and sieving: and (3) reducing the water content of the fermented material in the step 2) to be below 20%, and then carrying out crushing and sieving treatment to obtain a semi-finished product of the enzyme microorganism seed dressing agent special for potatoes.
4) And (4) qualified detection: and (3) detecting the seed dressing agent prepared in the step 3), wherein the qualified product is judged as the active beneficial bacteria of more than or equal to 2.0 hundred million/g, and the qualified seed dressing agent is subpackaged and warehoused.
A use method of an enzyme microorganism seed dressing agent comprises the following steps of mixing the prepared seed dressing agent with seed potatoes or ginger blocks according to a ratio of 1: 25-37, placing the mixture in an earthen bed for germination acceleration, and if needing placing a turnover basket, optimally placing a big bag in the turnover basket for heat preservation and moisture preservation, opening 10 holes on the big bag to keep ventilation, stacking the mixed seed potato blocks or ginger blocks and sandy soil in the turnover basket according to a layer of sandy soil and a layer of potato blocks or ginger blocks, wherein the optimal ratio of the sandy soil to the seed potato blocks or ginger blocks is 1: and 3, placing the mixture in an environment with the temperature of 15-20 ℃ for accelerating germination.
The following examples of potato seed dressing compare the growth conditions of the potato seeds using the patent, using the seed dressing agent and not using the seed dressing agent:
example two
The ferment microorganism seed dressing agent comprises the following raw materials in parts by weight:
0.10 part of ferment compound strain, 2 parts of rice bran or wheat bran, 4 parts of rice hull powder, 1.0 part of saw dust, 1.0 part of fishbone powder, 6 parts of shale, 2 parts of zeolite, 4 parts of grass carbon and 0.10 part of brown sugar.
The ferment composite bacteria strain has live bacteria amount more than 10 hundred million/g, contains seven beneficial bacteria of bacillus subtilis, saccharomyces cerevisiae, lactobacillus plantarum, streptomyces, aspergillus oryzae, trichoderma viride and fomes fomentarius, and has the proportion of 35 percent according to the bacillus subtilis, saccharomycetes, lactobacillus, actinomycetes and filamentous fungi: 35%: 10%: 10%: 10 percent.
A preparation method and a use method of an enzyme microorganism seed dressing agent, which are implemented in the same way as the first embodiment and are not described herein again.
EXAMPLE III
The ferment microorganism seed dressing agent comprises the following raw materials in parts by weight:
0.15 part of ferment compound strain, 1 part of rice bran or wheat bran, 5 parts of rice hull powder, 1.5 parts of saw dust, 0.5 part of fishbone powder, 7 parts of shale, 1 part of zeolite, 5 parts of grass carbon and 0.05 part of brown sugar.
The ferment composite bacteria strain has live bacteria amount more than 10 hundred million/g, contains seven beneficial bacteria of bacillus subtilis, saccharomyces cerevisiae, lactobacillus plantarum, streptomyces, aspergillus oryzae, trichoderma viride and fomes fomentarius, and has the proportion of 25 percent according to the bacillus subtilis, the saccharomycetes, the lactobacillus, the actinomycetes and the filamentous fungi: 25%: 20%: 15%: 15 percent.
A preparation method and a use method of an enzyme microorganism seed dressing agent, which are implemented in the same way as the first embodiment and are not described herein again.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (5)
1. The enzyme microorganism seed dressing agent is characterized by comprising the following raw materials in parts by weight:
0.05-0.15 part of ferment compound strain, 1-3 parts of rice bran or wheat bran, 3-5 parts of rice hull powder, 0.5-1.5 parts of saw dust, 0.5-1.5 parts of fishbone powder, 5-7 parts of shale, 1-3 parts of zeolite, 2-5 parts of grass peat and 0.05-0.15 part of brown sugar;
the ferment composite bacteria strain has live bacteria amount more than 10 hundred million/g, and comprises at least one of bacteria, fungi and actinomycetes in seven beneficial bacteria of bacillus subtilis, saccharomyces cerevisiae, lactobacillus plantarum, streptomyces, aspergillus oryzae, trichoderma viride and fomes fomentarius, wherein the ratio of the bacteria, the fungi and the actinomycetes is 40-60%: 20-30%: 20 to 30 percent.
2. The enzyme microorganism seed dressing agent according to claim 1, which is prepared from the following raw materials in parts by weight:
0.10 part of ferment compound strain, 2 parts of rice bran or wheat bran, 4 parts of rice hull powder, 1.0 part of saw dust, 1.0 part of fishbone powder, 6 parts of shale, 2 parts of zeolite, 4 parts of grass carbon and 0.10 part of brown sugar;
the ferment composite bacteria strain has live bacteria amount more than 10 hundred million/g, contains seven beneficial bacteria of bacillus subtilis, saccharomyces cerevisiae, lactobacillus plantarum, streptomyces, aspergillus oryzae, trichoderma viride and fomes fomentarius, and has the proportion of 25 percent according to the bacillus subtilis, saccharomycetes, lactobacillus, actinomycetes and filamentous fungi: 25%: 20%: 15%: 15 percent.
3. The preparation method of the enzyme microorganism seed dressing agent is characterized by comprising the following specific steps of:
1) preparation of fermentation materials: mixing the raw materials as recited in claim 1, wherein the ratio of carbon to nitrogen in the mixture is controlled to be 25: 1-40: 1;
2) aerobic fermentation: adding water to control the water content of the mixture to be about 55%, uniformly mixing, performing aerobic fermentation, turning the pile for the first time when the temperature in the pile reaches 50 ℃, turning the pile for every 7-10 days, and stopping fermentation until the temperature is reduced to about 30 ℃ and tends to be stable;
3) crushing and sieving: reducing the water content of the material fermented in the step 2) to below 20%, and then carrying out crushing and sieving treatment to obtain a semi-finished product of the enzyme microorganism seed dressing agent;
4) and (4) qualified detection: and (3) detecting the seed dressing agent prepared in the step 3), wherein the qualified product is judged as the active beneficial bacteria of more than or equal to 2.0 hundred million/g, and the qualified seed dressing agent is subpackaged and warehoused.
4. The use method of the enzyme microorganism seed dressing agent is characterized in that the prepared seed dressing agent and seed potatoes or ginger blocks are mixed according to the ratio of 1: mixing at a ratio of 25-37, and placing in earthen bed for germination.
5. The use method of the enzyme microorganism seed dressing agent according to claim 4, wherein if a transfer basket is required, a big bag is placed in the transfer basket for heat preservation and moisture preservation, the big bag is opened to keep ventilation, the mixed seed potato or ginger blocks and sandy soil are stacked in the transfer basket according to a layer of sandy soil and a layer of potato or ginger blocks, and the ratio of the sandy soil to the seed potato or ginger blocks is 1: and 3, placing the mixture in an environment with the temperature of 15-20 ℃ for accelerating germination.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111943760A (en) * | 2020-08-26 | 2020-11-17 | 山西农大绿荣科技有限公司 | Seed dressing agent for nitrogen-fixing bacterial manure of lichens |
| CN112573956A (en) * | 2020-12-25 | 2021-03-30 | 东北农业大学 | Seed dressing agent for promoting crop rooting and application thereof |
| CN112586511A (en) * | 2020-11-21 | 2021-04-02 | 东北农业大学 | Seed dressing agent for promoting germination of crop seeds planted in saline-alkali soil and seed treatment method |
-
2020
- 2020-06-17 CN CN202010553684.3A patent/CN111548975A/en not_active Withdrawn
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111943760A (en) * | 2020-08-26 | 2020-11-17 | 山西农大绿荣科技有限公司 | Seed dressing agent for nitrogen-fixing bacterial manure of lichens |
| CN112586511A (en) * | 2020-11-21 | 2021-04-02 | 东北农业大学 | Seed dressing agent for promoting germination of crop seeds planted in saline-alkali soil and seed treatment method |
| CN112586511B (en) * | 2020-11-21 | 2021-08-03 | 东北农业大学 | Seed dressing agent for promoting germination of crop seeds planted in saline-alkali soil and seed treatment method |
| CN112573956A (en) * | 2020-12-25 | 2021-03-30 | 东北农业大学 | Seed dressing agent for promoting crop rooting and application thereof |
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Application publication date: 20200818 |