CN108456021A - A kind of biological organic fertilizer and preparation method thereof with decomposed stalk effect - Google Patents
A kind of biological organic fertilizer and preparation method thereof with decomposed stalk effect Download PDFInfo
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- CN108456021A CN108456021A CN201810193365.9A CN201810193365A CN108456021A CN 108456021 A CN108456021 A CN 108456021A CN 201810193365 A CN201810193365 A CN 201810193365A CN 108456021 A CN108456021 A CN 108456021A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention discloses a kind of biological organic fertilizer and preparation method thereof with decomposed stalk effect, which includes the raw material of following parts by weight：35~45 parts of vinasse, 25~35 parts of animal wastes, 10~26 parts of straw powder, 0.01~0.05 part of compound microorganism ferments microbial inoculum.The biological organic fertilizer of the present invention can improve soil texture, increase the soil organism, meanwhile the microorganism mass propagation being inoculated with by prior fermentation organic fertilizer is present in organic fertilizer, and straw decomposing can be made to accelerate, organic matter and beneficial element are generated, nutriment is provided for plant growth.The metabolite that beneficial bacterium mass propagation generates in digest process can generate bacteriostasis to the harmful levels of pathogens in soil.
Description
Technical field
The invention belongs to biological product technical fields, specifically, being related to a kind of biology with decomposed stalk effect has
Machine fertilizer and preparation method thereof.
Background technology
With social development, the raising of living standard and environmental consciousness reduces the amount of application of chemical fertilizer, and organic food is increasingly
It has been favored by people.For a long time, people devote exclusive attention to output for simple, largely apply chemical fertilizer, cause soil fertility
Rapid decline, the extreme of effective element are uneven.Soil hardening, the poisonous metal largely harm such as accumulation, to exacerbate water and soil
Pollution.And application of organic fertilizers material can increase the organic substance of soil, to nutrient, energy, enzyme, the water in the structure of soil, soil
Point, ventilation and microbial activity be greatly improved, to increase soil vitality.Straw contain crude fibre, protein,
The elements such as calcium, phosphorus.If decomposed by nature merely, the period is too long, influences mechanical work.
Existing decomposing agent effect is single, has decomposed effect, peasant household's later stage also to need additional fertilizer, increase work just for stalk
The industry frequency and intensity.
Invention content
In view of this, the present invention provides a kind of biological organic fertilizer and preparation method thereof with decomposed stalk effect, this
The biological organic fertilizer of invention can improve soil texture, increase the soil organism, meanwhile, it is inoculated with by prior fermentation organic fertilizer
Microorganism mass propagation is present in organic fertilizer, straw decomposing can be made to accelerate, and generates organic matter and beneficial element, is given birth to for crop
It is long that nutriment is provided.The metabolite that beneficial bacterium mass propagation generates in digest process can generate the harmful levels of pathogens in soil
Bacteriostasis.
In order to solve the above-mentioned technical problem, the invention discloses a kind of biological organic fertilizer with decomposed stalk effect, packets
Include the raw material of following parts by weight：35~45 parts of vinasse, 25~35 parts of animal wastes, 10~26 parts of straw powder, complex microorganism hair
0.01~0.05 part of yeast-like fungi agent.
The compound microorganism ferments microbial inoculum includes following components according to mass parts：1-2 parts of bacillus subtilis, solution
1-2 parts of bacillus amyloliquefaciens, 1 part of bacillus laterosporus, 1-2 parts of bacillus pumilus, 1 part of Pichia pastoris, 1-2 parts of aspergillus niger.
Wherein, the biological organic fertilizer content of organic matter >=40%, total nutrient >=5.0%, moisture≤30%, ph value 5.5-
8.5, living bacteria count >=0.20 hundred million/gram.
The strain of compound microorganism ferments microbial inoculum includes：The content of bacillus subtilis is (3~8) × 108Cfu/g, solution
Bacillus amyloliquefaciens content is (3~5) × 108The content of cfu/g, bacillus laterosporus are (1~3) × 108Cfu/g, short and small bud
The content of spore bacillus is (4~9) × 108The content of cfu/g, Pichia pastoris are (1~3) × 108The content of cfu/g, aspergillus niger is
(5~10) × 108cfu/g；
The present invention also provides a kind of preparation methods of the biological organic fertilizer with decomposed stalk effect, include the following steps：
Step 1 prepares compound microorganism ferments microbial inoculum：
Step 1.1, seed activation：By bacillus subtilis, bacillus amyloliquefaciens, bacillus laterosporus, short and small gemma
Bacillus is crossed on eggplant bottle culture medium, 32-35 using beef-protein medium from one ring of seed test tube picking respectively
DEG C culture 24-48 hours, obtain the seed of shaking flask culture；Pichia pastoris uses PDA culture medium, from one ring of seed test tube picking,
It is crossed on eggplant bottle culture medium, 30-32 DEG C is cultivated 24-48 hours, and the seed of shaking flask culture is obtained；
Step 1.2 expands culture：Cultured eggplant bottle strain, each eggplant bottle are added sterile water, bacteria suspension are made,
So that its concentration is reached 10,000,000,000/ml, by 5% inoculum concentration, the 1000ml triangular flask culture mediums of the dress liquid 200ml for bacterium of having gone out are added
In, bacillus subtilis, bacillus amyloliquefaciens, bacillus laterosporus, 32-35 DEG C of bacillus pumilus, 180 revs/min
It is cultivated 16-18 hours on shaking table；Pichia pastoris is cultivated 16-18 hours on 30-32 DEG C, 180 revs/min of shaking table；
Step 1.3, fermentation tank culture：Cultured triangular flask culture solution is collected, prepares, go out by 5% inoculum concentration access
In the good fermentation medium of bacterium, 32 DEG C of temperature of control, ventilatory capacity 45m3/ hour, mixing speed 25r/mim are cultivated 24 hours, are gone out
Tank；
Step 1.4 presses zymotic fluid:Chaff powder=1:2(w：W) ratio, the zymotic fluid fermented is absorbed with chaff powder, and stirring is equal
Even, drying, drying；Make the water content of final chaff powder semi-finished product below 10%；
Step 1.5, detection：Dried bacterium powder semi-finished product, detected its every gram of effective bacterium number, reach 50-80 hundred million/gram；Point
Bacillus subtilis powder, bacillus amyloliquefaciens powder, bacillus laterosporus powder, bacillus pumilus powder, Bi Chi are not prepared
Yeast powder；
Step 1.6 weighs following components according to mass parts：1-2 parts of bacillus subtilis powder, bacillus amyloliquefaciens powder 1-
2 parts, 1 part of bacillus laterosporus powder, 1-2 parts of bacillus pumilus powder, 1 part of Pichia pastoris powder, 1-2 parts of aspergillus niger powder；
Step 1.7, by load weighted bacillus subtilis powder, bacillus amyloliquefaciens powder, bacillus laterosporus powder, short and small
Bacillus powder, Pichia pastoris powder and aspergillus niger powder are mixed, and compound microorganism ferments microbial inoculum is prepared；
Step 2 weighs：Following components is weighed according to parts by weight：35~45 parts of vinasse, 25~35 parts of animal waste, stalk
10~26 parts of powder, 0.01~0.05 part of compound microorganism ferments microbial inoculum；
Load weighted animal waste, vinasse, straw powder are uniformly mixed by step 3, and compound microbial culture starter is added, stirs
It mixes uniformly；The material prepared should be such that its water content is maintained between 55%-60%, have water without flowing out i.e. between clenching webs with hand
It can；Keep material loosening ventilative；
Step 4, windrow：Uniformly mixed material is piled into a pile, 80-120 centimeters of height, 2.0-2.5 meters of width, length
Degree is determined that section (since this fermentation is aerobic fermentation, is not necessarily to epiphragma, need to only prevent in rainy day plastic covering film to be trapezoidal by place
It only intakes).
Step 5, heap good pile after 2-3 days temperature reach 70 DEG C or so, using small forklift carry out turning, the object after turning
Material piles a pile again, and 80-120 centimeters of height, 2.0-2.5 meters of width, for length by place decision, section is trapezoidal (due to this hair
Ferment is aerobic fermentation, is not necessarily to epiphragma, need to only be prevented into water in rainy day plastic covering film)；At this point, material answer it is ordorless.
Step 6 when heap temperature reaches 70 DEG C after 2 days, then carries out turning operation.At this point, material color becomes brown, have denseer
Ammonia nitrogen smell.
At a pile type, stirring after 1 day is carried out continuously 3-4 days for step 7, again windrow.Windrow carries out later stage fermentation.
Step 8, after 7-10 days, can completely it is decomposed good, carry out spreading drying out；
Step 9 air-dries and smashes sieving：Raw material after fermentation is transported into outdoor threshing ground, dry naturally (or drying machine
Drying) after ensure that water content reaches 30% and terminates hereinafter, air-drying, will it is air-dried after raw material crushed by pulverizer after pass through again
Sieve sieving decontamination, the as biological organic fertilizer with decomposed stalk effect.
Compared with prior art, the present invention can be obtained including following technique effect：
The biological organic fertilizer of the present invention is the volume increase increment effect that can reach to crops, and can make stalk because wherein containing
The effective microbe strain of quick composting can make the quick decomposition of the organic matters such as farmland stalk, remove peasant household from harvest
Independent processing to leftover bits and pieces such as stalks.
Certainly, it implements any of the products of the present invention it is not absolutely required to while reaching all the above technique effect.
Specific implementation mode
Carry out the embodiment that the present invention will be described in detail below in conjunction with embodiment, thereby to the present invention how application technology hand
Section solves technical problem and reaches the realization process of technical effect to fully understand and implement.
The invention discloses a kind of biological organic fertilizers with decomposed stalk effect, include the raw material of following parts by weight：Wine
35~45 parts of grain, 25~35 parts of animal wastes, 10~26 parts of straw powder, 0.01~0.05 part of compound microorganism ferments microbial inoculum.
The compound microorganism ferments microbial inoculum includes following components according to mass parts：1-2 parts of bacillus subtilis, solution
1-2 parts of bacillus amyloliquefaciens, 1 part of bacillus laterosporus, 1-2 parts of bacillus pumilus, 1 part of Pichia pastoris, 1-2 parts of aspergillus niger.
Wherein, the biological organic fertilizer content of organic matter >=40%, total nutrient >=5.0%, moisture≤30%, ph value 5.5-
8.5, living bacteria count >=0.20 hundred million/gram.
The strain of compound microorganism ferments microbial inoculum includes：The content of bacillus subtilis is (3~8) × 108Cfu/g, solution
Bacillus amyloliquefaciens content is (3~5) × 108The content of cfu/g, bacillus laterosporus are (1~3) × 108Cfu/g, short and small bud
The content of spore bacillus is (4~9) × 108The content of cfu/g, Pichia pastoris are (1~3) × 108The content of cfu/g, aspergillus niger is
(5~10) × 108cfu/g；
The present invention also provides a kind of preparation methods of the biological organic fertilizer with decomposed stalk effect, include the following steps：
Step 1 prepares compound microorganism ferments microbial inoculum：
Step 1.1, seed activation：By bacillus subtilis, bacillus amyloliquefaciens, bacillus laterosporus, short and small gemma
Bacillus is crossed on eggplant bottle culture medium, 32-35 using beef-protein medium from one ring of seed test tube picking respectively
DEG C culture 24-48 hours, obtain the seed of shaking flask culture；Pichia pastoris uses PDA culture medium, from one ring of seed test tube picking,
It is crossed on eggplant bottle culture medium, 30-32 DEG C is cultivated 24-48 hours, and the seed of shaking flask culture is obtained；
Step 1.2 expands culture：Cultured eggplant bottle strain, each eggplant bottle are added sterile water, bacteria suspension are made,
So that its concentration is reached 10,000,000,000/ml, by 5% inoculum concentration, the 1000ml triangular flask culture mediums of the dress liquid 200ml for bacterium of having gone out are added
In, bacillus subtilis, bacillus amyloliquefaciens, bacillus laterosporus, 32-35 DEG C of bacillus pumilus, 180 revs/min
It is cultivated 16-18 hours on shaking table；Pichia pastoris is cultivated 16-18 hours on 30-32 DEG C, 180 revs/min of shaking table；
Step 1.3, fermentation tank culture：Cultured triangular flask culture solution is collected, prepares, go out by 5% inoculum concentration access
In the good fermentation medium of bacterium, 32 DEG C of temperature of control, ventilatory capacity 45m3/ hour, mixing speed 25r/mim are cultivated 24 hours, are gone out
Tank；
Step 1.4 presses zymotic fluid:Chaff powder=1:2(w：W) ratio, the zymotic fluid fermented is absorbed with chaff powder, and stirring is equal
Even, drying, drying；Make the water content of final chaff powder semi-finished product below 10%；
Step 1.5, detection：Dried bacterium powder semi-finished product, detected its every gram of effective bacterium number, reach 50-80 hundred million/gram；Point
Bacillus subtilis powder, bacillus amyloliquefaciens powder, bacillus laterosporus powder, bacillus pumilus powder, Bi Chi are not prepared
Yeast powder；
Step 1.6 weighs following components according to mass parts：1-2 parts of bacillus subtilis powder, bacillus amyloliquefaciens powder 1-
2 parts, 1 part of bacillus laterosporus powder, 1-2 parts of bacillus pumilus powder, 1 part of Pichia pastoris powder, 1-2 parts of aspergillus niger powder；
Step 1.7, by load weighted bacillus subtilis powder, bacillus amyloliquefaciens powder, bacillus laterosporus powder, short and small
Bacillus powder, Pichia pastoris powder and aspergillus niger powder are mixed, and compound microorganism ferments microbial inoculum is prepared；
Step 2 weighs：Following components is weighed according to parts by weight：35~45 parts of vinasse, 25~35 parts of animal waste, stalk
10~26 parts of powder, 0.01~0.05 part of compound microorganism ferments microbial inoculum；
Load weighted animal waste, vinasse, straw powder are uniformly mixed by step 3, and compound microbial culture starter is added, stirs
It mixes uniformly；The material prepared should be such that its water content is maintained between 55%-60%, have water without flowing out i.e. between clenching webs with hand
It can；Keep material loosening ventilative；
Step 4, windrow：Uniformly mixed material is piled into a pile, 80-120 centimeters of height, 2.0-2.5 meters of width, length
It is determined by place, section is trapezoidal (since this fermentation is aerobic fermentation, without epiphragma, need to only be prevented in rainy day plastic covering film
Water inlet).
Step 5, heap good pile after 2-3 days temperature reach 70 DEG C or so, using small forklift carry out turning, the object after turning
Material piles a pile again, and 80-120 centimeters of height, 2.0-2.5 meters of width, for length by place decision, section is trapezoidal (due to this hair
Ferment is aerobic fermentation, is not necessarily to epiphragma, need to only be prevented into water in rainy day plastic covering film)；At this point, material answer it is ordorless.
Step 6 when heap temperature reaches 70 DEG C after 2 days, then carries out turning operation.At this point, material color becomes brown, have denseer
Ammonia nitrogen smell.
At a pile type, stirring after 1 day is carried out continuously 3-4 days for step 7, again windrow.Windrow carries out later stage fermentation.
Step 8, after 7-10 days, can completely it is decomposed good, carry out spreading drying out；
Step 9 air-dries and smashes sieving：Raw material after fermentation is transported into outdoor threshing ground, dry naturally (or drying machine
Drying) after ensure that water content reaches 30% and terminates hereinafter, air-drying, will it is air-dried after raw material crushed by pulverizer after pass through again
Sieve sieving decontamination, the as biological organic fertilizer with decomposed stalk effect.
Embodiment 1
A kind of preparation method of the biological organic fertilizer with decomposed stalk effect, includes the following steps：
Step 1 prepares compound microorganism ferments microbial inoculum：
Step 1.1, seed activation：By bacillus subtilis, bacillus amyloliquefaciens, bacillus laterosporus, short and small gemma
Bacillus is crossed to from one ring of seed test tube picking on eggplant bottle culture medium using beef-protein medium respectively, 34 DEG C of trainings
It supports 36 hours, obtains the seed of shaking flask culture；Pichia pastoris is crossed to eggplant using PDA culture medium from one ring of seed test tube picking
On sub- bottle culture medium, 30 DEG C are cultivated 36 hours, and the seed of shaking flask culture is obtained；
Step 1.2 expands culture：Cultured eggplant bottle strain, each eggplant bottle are added sterile water, bacteria suspension are made,
So that its concentration is reached 10,000,000,000/ml, by 5% inoculum concentration, the 1000ml triangular flask culture mediums of the dress liquid 200ml for bacterium of having gone out are added
In, bacillus subtilis, bacillus amyloliquefaciens, bacillus laterosporus, 34 DEG C of bacillus pumilus, 180 revs/min of shaking table
Upper culture 17 hours；Pichia pastoris is cultivated 17 hours on 30 DEG C, 180 revs/min of shaking table；
Step 1.3, fermentation tank culture：Cultured triangular flask culture solution is collected, prepares, go out by 5% inoculum concentration access
In the good fermentation medium of bacterium, 32 DEG C of temperature of control, ventilatory capacity 45m3/ hour, mixing speed 25r/mim are cultivated 24 hours, are gone out
Tank；
Step 1.4 presses zymotic fluid:Chaff powder=1:2(w：W) ratio, the zymotic fluid fermented is absorbed with chaff powder, and stirring is equal
Even, drying, drying；Make the water content of final chaff powder semi-finished product below 10%；
Step 1.5, detection：Bacterium powder semi-finished product have been dried, its every gram of effective bacterium number is detected, reach 6,500,000,000/gram；Respectively
Bacillus subtilis powder, bacillus amyloliquefaciens powder, bacillus laterosporus powder, bacillus pumilus powder, complete red ferment is prepared
Female powder；
Step 1.6 weighs following components according to mass parts：1 part of bacillus subtilis powder, 2 parts of bacillus amyloliquefaciens powder,
1 part of bacillus laterosporus powder, 1 part of bacillus pumilus powder, 1 part of Pichia pastoris powder, 2 parts of aspergillus niger powder；
Step 1.7, by load weighted bacillus subtilis powder, bacillus amyloliquefaciens powder, bacillus laterosporus powder, short and small
Bacillus powder, Pichia pastoris powder and aspergillus niger powder are mixed, and compound microorganism ferments microbial inoculum is prepared；
Step 2 weighs：Following components is weighed according to parts by weight：40 parts of vinasse, 30 parts of animal waste, 20 parts of straw powder,
0.03 part of compound microorganism ferments microbial inoculum；
Load weighted animal waste, vinasse, straw powder are uniformly mixed by step 3, and compound microbial culture starter is added, stirs
It mixes uniformly；The material prepared should make its water content be maintained at 58%, have water without outflow between clenching webs with hand；It keeps
Material loosening is ventilative；
Step 4, windrow：Uniformly mixed material is piled into a pile, 100 centimeters of height, 2.2 meters of width, length is by place
It determines, section is trapezoidal (since this fermentation is aerobic fermentation, is not necessarily to epiphragma, need to only be prevented into water in rainy day plastic covering film).
Step 5, heap good pile after 2-3 days temperature reach 70 DEG C or so, using small forklift carry out turning, the object after turning
Material piles a pile again, and 100 centimeters of height, 2.3 meters of width, for length by place decision, section is trapezoidal (since this fermentation is aerobic
Fermentation is not necessarily to epiphragma, need to only be prevented into water in rainy day plastic covering film)；At this point, material answer it is ordorless.
Step 6 when heap temperature reaches 70 DEG C after 2 days, then carries out turning operation.At this point, material color becomes brown, have denseer
Ammonia nitrogen smell.
At a pile type, stirring after 1 day is carried out continuously 3-4 days for step 7, again windrow；Windrow carries out later stage fermentation.
Step 8, after 7-10 days, can completely it is decomposed good, carry out spreading drying out；
Step 9 air-dries and smashes sieving：Raw material after fermentation is transported into outdoor threshing ground, dry naturally (or drying machine
Drying) after ensure that water content reaches 30% and terminates hereinafter, air-drying, will it is air-dried after raw material crushed by pulverizer after pass through again
Sieve sieving decontamination, the as biological organic fertilizer with decomposed stalk effect.
Embodiment 2
A kind of preparation method of the biological organic fertilizer with decomposed stalk effect, includes the following steps：
Step 1 prepares compound microorganism ferments microbial inoculum：
Step 1.1, seed activation：By bacillus subtilis, bacillus amyloliquefaciens, bacillus laterosporus, short and small gemma
Bacillus is crossed to from one ring of seed test tube picking on eggplant bottle culture medium using beef-protein medium respectively, 32 DEG C of trainings
It supports 48 hours, obtains the seed of shaking flask culture；Pichia pastoris is crossed to eggplant using PDA culture medium from one ring of seed test tube picking
On sub- bottle culture medium, 30 DEG C are cultivated 48 hours, and the seed of shaking flask culture is obtained；
Step 1.2 expands culture：Cultured eggplant bottle strain, each eggplant bottle are added sterile water, bacteria suspension are made,
So that its concentration is reached 10,000,000,000/ml, by 5% inoculum concentration, the 1000ml triangular flask culture mediums of the dress liquid 200ml for bacterium of having gone out are added
In, bacillus subtilis, bacillus amyloliquefaciens, bacillus laterosporus, 32 DEG C of bacillus pumilus, 180 revs/min of shaking table
Upper culture 18 hours；Pichia pastoris is cultivated 18 hours on 30 DEG C, 180 revs/min of shaking table；
Step 1.3, fermentation tank culture：Cultured triangular flask culture solution is collected, prepares, go out by 5% inoculum concentration access
In the good fermentation medium of bacterium, 32 DEG C of temperature of control, ventilatory capacity 45m3/ hour, mixing speed 25r/mim are cultivated 24 hours, are gone out
Tank；
Step 1.4 presses zymotic fluid:Chaff powder=1:2(w：W) ratio, the zymotic fluid fermented is absorbed with chaff powder, and stirring is equal
Even, drying, drying；Make the water content of final chaff powder semi-finished product below 10%；
Step 1.5, detection：Bacterium powder semi-finished product have been dried, its every gram of effective bacterium number is detected, reach 5,000,000,000/gram；Respectively
Bacillus subtilis powder, bacillus amyloliquefaciens powder, bacillus laterosporus powder, bacillus pumilus powder, complete red ferment is prepared
Female powder；
Step 1.6 weighs following components according to mass parts：1 part of bacillus subtilis powder, 2 parts of bacillus amyloliquefaciens powder,
1 part of bacillus laterosporus powder, 1 part of bacillus pumilus powder, 1 part of Pichia pastoris powder, 2 parts of aspergillus niger powder；
Step 1.7, by load weighted bacillus subtilis powder, bacillus amyloliquefaciens powder, bacillus laterosporus powder, short and small
Bacillus powder, Pichia pastoris powder and aspergillus niger powder are mixed, and compound microorganism ferments microbial inoculum is prepared；
Step 2 weighs：Following components is weighed according to parts by weight：35 parts of vinasse, 35 parts of animal waste, 10 parts of straw powder,
0.05 part of compound microorganism ferments microbial inoculum；
Load weighted animal waste, vinasse, straw powder are uniformly mixed by step 3, and compound microbial culture starter is added, stirs
It mixes uniformly；The material prepared should make its water content be maintained at 55%, have water without outflow between clenching webs with hand；It keeps
Material loosening is ventilative；
Step 4, windrow：Uniformly mixed material is piled into a pile, 80 centimeters of height, 2.0 meters of width, length is by place
It determines, section is trapezoidal (since this fermentation is aerobic fermentation, is not necessarily to epiphragma, need to only be prevented into water in rainy day plastic covering film).
Step 5, heap good pile after 2-3 days temperature reach 70 DEG C or so, using small forklift carry out turning, the object after turning
Material piles a pile again, and 80 centimeters of height, 2.0 meters of width, for length by place decision, section is trapezoidal (since this fermentation is aerobic
Fermentation is not necessarily to epiphragma, need to only be prevented into water in rainy day plastic covering film)；At this point, material answer it is ordorless.
Step 6 when heap temperature reaches 70 DEG C after 2 days, then carries out turning operation.At this point, material color becomes brown, have denseer
Ammonia nitrogen smell.
At a pile type, stirring after 1 day is carried out continuously 3-4 days for step 7, again windrow；Windrow carries out later stage fermentation.
Step 8, after 7-10 days, can completely it is decomposed good, carry out spreading drying out；
Step 9 air-dries and smashes sieving：Raw material after fermentation is transported into outdoor threshing ground, dry naturally (or drying machine
Drying) after ensure that water content reaches 30% and terminates hereinafter, air-drying, will it is air-dried after raw material crushed by pulverizer after pass through again
Sieve sieving decontamination, the as biological organic fertilizer with decomposed stalk effect.
Embodiment 3
A kind of preparation method of the biological organic fertilizer with decomposed stalk effect, includes the following steps：
Step 1 prepares compound microorganism ferments microbial inoculum：
Step 1.1, seed activation：By bacillus subtilis, bacillus amyloliquefaciens, bacillus laterosporus, short and small gemma
Bacillus is crossed to from one ring of seed test tube picking on eggplant bottle culture medium using beef-protein medium respectively, 35 DEG C of trainings
It supports 24 hours, obtains the seed of shaking flask culture；Pichia pastoris is crossed to eggplant using PDA culture medium from one ring of seed test tube picking
On sub- bottle culture medium, 32 DEG C are cultivated 24 hours, and the seed of shaking flask culture is obtained；
Step 1.2 expands culture：Cultured eggplant bottle strain, each eggplant bottle are added sterile water, bacteria suspension are made,
So that its concentration is reached 10,000,000,000/ml, by 5% inoculum concentration, the 1000ml triangular flask culture mediums of the dress liquid 200ml for bacterium of having gone out are added
In, bacillus subtilis, bacillus amyloliquefaciens, bacillus laterosporus, 35 DEG C of bacillus pumilus, 180 revs/min of shaking table
Upper culture 16 hours；Pichia pastoris is cultivated 16 hours on 32 DEG C, 180 revs/min of shaking table；
Step 1.3, fermentation tank culture：Cultured triangular flask culture solution is collected, prepares, go out by 5% inoculum concentration access
In the good fermentation medium of bacterium, 32 DEG C of temperature of control, ventilatory capacity 45m3/ hour, mixing speed 25r/mim are cultivated 24 hours, are gone out
Tank；
Step 1.4 presses zymotic fluid:Chaff powder=1:2(w：W) ratio, the zymotic fluid fermented is absorbed with chaff powder, and stirring is equal
Even, drying, drying；Make the water content of final chaff powder semi-finished product below 10%；
Step 1.5, detection：Bacterium powder semi-finished product have been dried, its every gram of effective bacterium number is detected, reach 8,000,000,000/gram；Respectively
Bacillus subtilis powder, bacillus amyloliquefaciens powder, bacillus laterosporus powder, bacillus pumilus powder, complete red ferment is prepared
Female powder；
Step 1.6 weighs following components according to mass parts：2 parts of bacillus subtilis powder, 1 part of bacillus amyloliquefaciens powder,
1 part of bacillus laterosporus powder, 2 parts of bacillus pumilus powder, 1 part of Pichia pastoris powder, 1 part of aspergillus niger powder；
Step 1.7, by load weighted bacillus subtilis powder, bacillus amyloliquefaciens powder, bacillus laterosporus powder, short and small
Bacillus powder, Pichia pastoris powder and aspergillus niger powder are mixed, and compound microorganism ferments microbial inoculum is prepared；
Step 2 weighs：Following components is weighed according to parts by weight：45 parts of vinasse, 25 parts of animal waste, 26 parts of straw powder,
0.01 part of compound microorganism ferments microbial inoculum；
Load weighted animal waste, vinasse, straw powder are uniformly mixed by step 3, and compound microbial culture starter is added, stirs
It mixes uniformly；The material prepared should make its water content be maintained at 60%, have water without outflow between clenching webs with hand；It keeps
Material loosening is ventilative；
Step 4, windrow：Uniformly mixed material is piled into a pile, 120 centimeters of height, 2.5 meters of width, length is by place
It determines, section is trapezoidal (since this fermentation is aerobic fermentation, is not necessarily to epiphragma, need to only be prevented into water in rainy day plastic covering film).
Step 5, heap good pile after 2-3 days temperature reach 70 DEG C or so, using small forklift carry out turning, the object after turning
Material piles a pile again, and 120 centimeters of height, 2.5 meters of width, for length by place decision, section is trapezoidal (since this fermentation is aerobic
Fermentation is not necessarily to epiphragma, need to only be prevented into water in rainy day plastic covering film)；At this point, material answer it is ordorless.
Step 6 when heap temperature reaches 70 DEG C after 2 days, then carries out turning operation.At this point, material color becomes brown, have denseer
Ammonia nitrogen smell.
At a pile type, stirring after 1 day is carried out continuously 3-4 days for step 7, again windrow.Windrow carries out later stage fermentation.
Step 8, after 7-10 days, can completely it is decomposed good, carry out spreading drying out；
Step 9 air-dries and smashes sieving：Raw material after fermentation is transported into outdoor threshing ground, dry naturally (or drying machine
Drying) after ensure that water content reaches 30% and terminates hereinafter, air-drying, will it is air-dried after raw material crushed by pulverizer after pass through again
Sieve sieving decontamination, the as biological organic fertilizer with decomposed stalk effect.
Illustrate the technique effect of the present invention with reference to specific experimental data：
1, influence of this product to yields of vegetables
Cell is tested, and the soil for selecting a fertilizer uniform, smooth is tested.It is total that this experiment sets 4 processing, 3 repetitions altogether
12 cell random alignments, 33.3 ㎡ of plot area, experimental field surrounding is equipped with 2.5 meters of guarantor's row.
Handle 1. Routine control CK1 (peasant habit fertilising).
2. Routine control+capsicum applies 200kg/ mus of works of biological organic fertilizer that embodiment 1 is prepared before transplanting for processing
Base manure.
3. CK2 Routine controls+capsicum applies the biological organic fertilizer inactivation matrix that embodiment 1 is prepared before transplanting for processing
200kg/ mus are made base manure.
4. CK3 Routine controls+capsicum makees base manure before transplanting using 200kg/ mus of equivalent fine sand for processing.As a result such as the following table 1：
Table 1
The experimental results showed that after using this biological organic fertilizer, 3379kg/ mus of average yield per mu, respectively than processing 1, processing 3,
Processing 4 volume increase 394kg, 320kg, 358kg, rate of growth 13.2%, 10.5%, 11.9.There is preferable production-increasing function.
2, influence of this product to straw decomposing effect
Water is added to activate 16 hours by 0.3% additive amount finished product, the liquid after activation is uniformly sprayed on stalk.Simultaneously
It compares, sprays same amount of clear water on stalk.Two groups of experiments are placed under the same conditions, daily Timing measurement temperature, wet
The decomposed degree of degree and stalk.After 2 days, experiment stalk heap starts to warm up, up to 50 DEG C, and microorganism starts mass propagation, and 9
Stalk starts a large amount of decompositions after it.By Experimental comparison, comparative experiments group and control group straw in terms of color, feel, stretching resistance
The decomposed degree of stalk, experimental group are better than control group.The variation of stalk weight reflects straw decomposing degree, by 15 days from side
It is decomposed, the experimental results showed that, under the same terms, experimental group stalk weight ratio experiment start when reduce 50%；Control group is reduced
25%, this is also indicated that, after this biological organic fertilizer is added, the decomposed speed of stalk is more much faster than naturally decomposed.
Above description has shown and described several preferred embodiments of invention, but as previously described, it should be understood that invention is not
It is confined to form disclosed herein, is not to be taken as excluding other embodiments, and can be used for various other combinations, modification
And environment, and can be carried out by the above teachings or related fields of technology or knowledge in the scope of the invention is set forth herein
Change.And changes and modifications made by those skilled in the art do not depart from the spirit and scope of invention, then should all be weighed appended by invention
In the protection domain that profit requires.
Claims (10)
1. a kind of biological organic fertilizer with decomposed stalk effect, which is characterized in that include the raw material of following parts by weight：Vinasse 35
~45 parts, 25~35 parts of animal waste, 10~26 parts of straw powder, 0.01~0.05 part of compound microorganism ferments microbial inoculum.
2. the biological organic fertilizer according to claim 1 with decomposed stalk effect, which is characterized in that described is compound micro-
Bio-fermentation agents include following components according to mass parts：1-2 parts of bacillus subtilis, 1-2 parts of bacillus amyloliquefaciens, side spore
1 part of bacillus, 1-2 parts of bacillus pumilus, 1 part of Pichia pastoris, 1-2 parts of aspergillus niger.
3. the biological organic fertilizer according to claim 1 with decomposed stalk effect, which is characterized in that the biological organic fertilizer
The content of organic matter >=40%, total nutrient >=5.0%, moisture≤30%, ph value 5.5-8.5, living bacteria count >=0.20 hundred million/gram.
4. a kind of preparation method of the biological organic fertilizer with decomposed stalk effect, which is characterized in that include the following steps：
Step 1 prepares compound microorganism ferments microbial inoculum；
Step 2 weighs：Following components is weighed according to parts by weight：35~45 parts of vinasse, 25~35 parts of animal waste, straw powder 10
~26 parts, 0.01~0.05 part of compound microorganism ferments microbial inoculum；
Step 3 mixes in proportion animal waste, vinasse, straw powder, and compound microbial culture starter is added, stirs evenly,
There is water without flowing out between clenching webs with hand, and keeps material loosening ventilative；
Step 4, windrow：Uniformly mixed material is piled into a pile, length is determined by place, and section is trapezoidal, is covered in the rainy day
Plastic foil；
Step 5, heap good pile after 2-3 days temperature reach 70 DEG C, carry out turning with small forklift, the material after turning piles item again
Pile；
Step 6 when heap temperature reaches 70 DEG C after 2 days, then carries out turning operation；At this point, material color becomes brown, there is denseer ammonia
Nitrogen taste；
At a pile type, stirring after 1 day is carried out continuously 3-4 days for step 7, again windrow；Windrow carries out later stage fermentation；
Step 8, after 7-10 days, completely it is decomposed good, carry out spreading drying out；
Step 9 air-dries and smashes sieving：Raw material after fermentation is transported into outdoor threshing ground, dry naturally or drying machine drying after
Ensure that water content reaches 30% and terminates hereinafter, air-drying, again by sieve mistake after the raw material after air-drying is crushed by pulverizer
Weed out impurity, the as biological organic fertilizer with decomposed stalk effect.
5. preparation method according to claim 4, which is characterized in that described to prepare compound microorganism ferments microbial inoculum specific
For：
Step 1.1, seed activation：By bacillus subtilis, bacillus amyloliquefaciens, bacillus laterosporus, bacillus pumilus
It is crossed on eggplant bottle culture medium and is cultivated, obtained from one ring of seed test tube picking using beef-protein medium respectively
To the seed of shaking flask culture；Pichia pastoris is crossed to eggplant bottle culture medium using PDA culture medium from one ring of seed test tube picking
On cultivated, obtain the seed of shaking flask culture；
Step 1.2 expands culture：Cultured eggplant bottle strain, each eggplant bottle are added 100ml sterile waters, bacteria suspension are made,
By 5% inoculum concentration, it is added in the 1000ml triangular flask culture mediums of dress liquid 200ml for bacterium of having gone out, by bacillus subtilis, Xie Dian
Afnyloliquefaciens, bacillus laterosporus, bacillus pumilus and Pichia pastoris are placed on shaking table and are cultivated；
Step 1.3, fermentation tank culture：Cultured triangular flask culture solution is collected, by 5% inoculum concentration access fermentation medium
It is cultivated, goes out tank；
Chaff powder is added in step 1.4 in zymotic fluid, and the zymotic fluid fermented is absorbed with chaff powder；It stirs evenly, dry, dry；Make
The water content of final chaff powder semi-finished product is below 10%；
Step 1.5, detection:Dried bacterium powder semi-finished product, detected its every gram of effective bacterium number, reach 50-80 hundred million/gram；It makes respectively
It is standby to obtain bacillus subtilis powder, bacillus amyloliquefaciens powder, bacillus laterosporus powder, bacillus pumilus powder, Pichia pastoris
Powder；
Step 1.6 weighs following components according to mass parts：1-2 parts of bacillus subtilis powder, 1-2 parts of bacillus amyloliquefaciens powder,
1 part of bacillus laterosporus powder, 1-2 parts of bacillus pumilus powder, 1 part of Pichia pastoris powder, 1-2 parts of aspergillus niger powder；
Step 1.7, by load weighted bacillus subtilis powder, bacillus amyloliquefaciens powder, bacillus laterosporus powder, short and small gemma
Bacillus powder, Pichia pastoris powder and aspergillus niger powder are mixed, and compound microorganism ferments microbial inoculum is prepared.
6. preparation method according to claim 5, which is characterized in that bacillus subtilis, Xie Dian in the step 1.1
Afnyloliquefaciens, bacillus laterosporus, bacillus pumilus cultivation temperature be 32-35 DEG C, incubation time 24-48h；Bi Chi
The cultivation temperature of yeast is 30-32 DEG C, incubation time 24-48h.
7. preparation method according to claim 5, which is characterized in that a concentration of 100 of bacteria suspension in the step 1.2
Hundred million/ml, bacillus subtilis, bacillus amyloliquefaciens, bacillus laterosporus, bacillus pumilus cultivation temperature be 32-35
DEG C, incubation time is 16-18 hours, and shaking speed is 180 revs/min, and the cultivation temperature of Pichia pastoris is 30-32 DEG C, culture
Time is 16-18 hours, and shaking speed is 180 revs/min.
8. preparation method according to claim 5, which is characterized in that the temperature of the fermentation tank in the step 1.3 is 32
DEG C, ventilatory capacity 45m3/ hour, mixing speed 25r/mim；Incubation time is 24 hours；Zymotic fluid in step 1.4 and chaff
The mass ratio of powder is 1:2.
9. preparation method according to claim 4, which is characterized in that the water content of the material stirred to get in step 3
For 55%-60%.
10. preparation method according to claim 4, which is characterized in that the height 80- of the pile in step 4 and step 5
120 centimeters, 2.0-2.5 meters of width.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110903112A (en) * | 2019-12-03 | 2020-03-24 | 威海金颐阳药业有限公司 | Organic fertilizer for American ginseng |
| CN111533586A (en) * | 2020-05-09 | 2020-08-14 | 李晟雪 | Chicken manure bio-organic fertilizer and preparation method thereof |
| CN111620745A (en) * | 2020-05-09 | 2020-09-04 | 大连理工大学 | Method for producing bio-organic fertilizer by degrading agricultural wastes with biological agent |
| CN113186126A (en) * | 2021-04-16 | 2021-07-30 | 天津益农生物科技有限公司 | Microbial agent |
| CN117185870A (en) * | 2023-08-14 | 2023-12-08 | 广东岭南大健康生态科技集团有限公司 | Biological organic fertilizer and application thereof in soil improvement of citrus planting |
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| CN102951950A (en) * | 2011-08-19 | 2013-03-06 | 訾然 | Production method for biological organic fertilizer |
| CN103819237A (en) * | 2014-02-07 | 2014-05-28 | 湖南泰谷生物科技股份有限公司 | Microbial fertilizer special for tobaccos, and preparing method thereof |
| CN104692846A (en) * | 2015-02-27 | 2015-06-10 | 山东省农业可持续发展研究所 | Straw bio-organic fertilizer and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102951950A (en) * | 2011-08-19 | 2013-03-06 | 訾然 | Production method for biological organic fertilizer |
| CN103819237A (en) * | 2014-02-07 | 2014-05-28 | 湖南泰谷生物科技股份有限公司 | Microbial fertilizer special for tobaccos, and preparing method thereof |
| CN104692846A (en) * | 2015-02-27 | 2015-06-10 | 山东省农业可持续发展研究所 | Straw bio-organic fertilizer and preparation method thereof |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110903112A (en) * | 2019-12-03 | 2020-03-24 | 威海金颐阳药业有限公司 | Organic fertilizer for American ginseng |
| CN111533586A (en) * | 2020-05-09 | 2020-08-14 | 李晟雪 | Chicken manure bio-organic fertilizer and preparation method thereof |
| CN111620745A (en) * | 2020-05-09 | 2020-09-04 | 大连理工大学 | Method for producing bio-organic fertilizer by degrading agricultural wastes with biological agent |
| CN111620745B (en) * | 2020-05-09 | 2021-08-24 | 大连理工大学 | Method for producing bio-organic fertilizer by degrading agricultural wastes with biological agent |
| CN111533586B (en) * | 2020-05-09 | 2022-06-07 | 李晟雪 | Chicken manure bio-organic fertilizer and preparation method thereof |
| CN113186126A (en) * | 2021-04-16 | 2021-07-30 | 天津益农生物科技有限公司 | Microbial agent |
| CN117185870A (en) * | 2023-08-14 | 2023-12-08 | 广东岭南大健康生态科技集团有限公司 | Biological organic fertilizer and application thereof in soil improvement of citrus planting |
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Application publication date: 20180828 |