CN115124389B - Preparation method of microbial agent composition for rice cultivation - Google Patents

Preparation method of microbial agent composition for rice cultivation Download PDF

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CN115124389B
CN115124389B CN202211002377.1A CN202211002377A CN115124389B CN 115124389 B CN115124389 B CN 115124389B CN 202211002377 A CN202211002377 A CN 202211002377A CN 115124389 B CN115124389 B CN 115124389B
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powder
cold
agent
parts
microbial agent
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CN115124389A (en
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公丽凤
冯建文
王金玲
卢志全
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Harbin Pigano Biotechnology Co ltd
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    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05B—PHOSPHATIC FERTILISERS
    • C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C05F11/02—Other organic fertilisers from peat, brown coal, and similar vegetable deposits
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80—Soil conditioners
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/11—Bacillus megaterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/12—Bacillus polymyxa ; Paenibacillus polymyxa
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Abstract

The application discloses a preparation method of a microbial agent composition for rice cultivation, which comprises the following raw materials: 20-200 parts of microbial agent; 1-4 parts of a cold-resistant agent; the microbial agent is at least one of a paenibacillus mucilaginosus agent, a rhodopseudomonas palustris agent, a bacillus subtilis agent, a bacillus megaterium agent and a paenibacillus polymyxa agent; the cold-resistant agent is at least one of desulfurized gypsum, seaweed powder and carotenoid. According to the application, through the synergistic effect of the microbial agent and the cold-resistant agent, the absorption of the cold-resistant agent by soil and crops is promoted, on one hand, the physicochemical property and the component structure of the soil are improved, and on the other hand, the cold-resistant performance of the crops is improved, so that the low-temperature environment can be better resisted, and the crops can grow normally.

Description

Preparation method of microbial agent composition for rice cultivation
The application relates to a microbial agent composition and application thereof in crop cultivation, and the application date of the original application is 2022, 1 month and 29 days, and the application number of the original application is 202210113081.0.
Technical Field
The application relates to the technical field of agricultural fertilizers, in particular to a microbial inoculant composition and application thereof in crop cultivation.
Background
The rice is one of the important grain crops in China and plays an important role in national economy. The growth of rice requires proper temperature, sufficient illumination, moisture and the like, and has certain requirements on the environment. The main production area of rice in China is northeast area, but the contradiction between low northeast air temperature and the preference of rice is one of the main problems of rice production. The temperature is low in early spring in northeast, which may cause insufficient accumulated temperature and delayed cold injury in the rice growth period, and seriously affect the yield and quality of rice. Meanwhile, physiological activities of rice are hindered, permeability change of cell membranes and capability of removing free radicals are weakened, photosynthesis is weakened, chlorophyll and antioxidant enzyme content is reduced, so that phenomena of weak seedlings, rotten seedlings, dead seedlings and the like occur, and huge economic loss is caused.
In the prior art, cold-resistant agents are usually added to reduce cold damage of crops, but most of the cold-resistant agents have poor application effects and cannot effectively reduce the cold damage loss of crops.
Disclosure of Invention
Aiming at the problems in the prior art, the application provides a microbial agent composition and application thereof in crop cultivation, so as to improve cold resistance, provide nutrients, promote growth, increase yield and improve quality of crops cultivated in cold regions, and improve and restore ecological environment of soil.
The microbial agent composition provided by the application comprises the following raw materials in parts by weight:
20-200 parts of microbial agent;
1-4 parts of a cold resistant agent;
the microbial agent is at least one of a paenibacillus mucilaginosus agent, a rhodopseudomonas palustris agent, a bacillus subtilis agent, a bacillus megaterium agent and a paenibacillus polymyxa agent;
the cold-resistant agent is at least one of desulfurized gypsum, seaweed powder and carotenoid.
The microbial agent has the effects of improving soil fertility, promoting crop growth, inducing crop resistance, promoting soil flora balance, improving soil physicochemical property, inhibiting harmful bacteria, improving crop quality and yield, and the like; the microbial agent is applied to crop soil, plays a remarkable fertilizer effect, can promote secretion of plant regulating hormone and absorption of nutrient elements, has the functions of fixing nitrogen, dissolving phosphorus, dissolving potassium and the like, thereby improving the capability of resisting or resisting cold injury of crops and ensuring normal growth of the crops. Although the microbial inoculum can not completely replace chemical fertilizers, the microbial inoculum can effectively reduce the dosage of fertilizers, improve the utilization rate of traditional fertilizers and has great significance in improving soil fertility, guaranteeing soil health, promoting crop growth and the like.
In the cold-resistant agent, the desulfurized gypsum releases more calcium ions under the action of microorganism bacteria, and the absorption of the calcium ions can strengthen crop nutrition and improve the cold-resistant performance of crops; the calcium ions can also promote the growth of microorganisms and provide growth factors for the microorganisms; the seaweed powder is rich in nutrients such as sugar, protein, amino acid, vitamin, mineral and the like, so that not only can the seaweed powder provide nutrients for microorganism bacteria and promote the microorganism to secrete plant growth regulating hormone, but also the cold resistance of crops can be improved after the seaweed powder is absorbed by the crops; the carotenoid is absorbed by crops under the action of microorganism bacteria, so that the tolerance of the crops to cold can be obviously increased, meanwhile, the carotenoid directly provides a precursor substance for synthesizing cold-resistant hormone for the microorganism bacteria, and the synthesized cold-resistant hormone can be absorbed by the crops, so that the stress resistance of the crops is improved.
The microbial agent and the cold-resistant agent have synergistic interaction, namely the life activity of the microbial agent strain can promote the release of components in the cold-resistant agent, so that the absorption efficiency of crops is improved, the cold-resistant agent can promote the growth of microbial bacteria and increase the secretion of cold-resistant hormones, and the cold-resistant hormones are absorbed by the crops, so that the cold resistance of the crops is further improved, and the cold resistance and cold resistance of the crops are obviously improved as a result of the combined action of the microbial agent strain and the cold-resistant agent.
The bacillus subtilis used in the application is obtained by separation and purification in soil, is identified as bacillus subtilis through morphology and 16sRNA, and is named as bacillus subtilis WJL-K8; the sources of other strains used in the application are purchased from China general microbiological culture collection center (CGMCC) and subjected to cold-resistant domestication, and the serial numbers of the strains are respectively as follows: bacillus megaterium, CGMCC 1.1870, rhodobacillus palustris, CGMCC1.2181, paenibacillus polymyxa, CGMCC 1.877, paenibacillus mucilaginosus and CGMCC 1.910.
The cold-resistant domestication process of the 5 strains comprises the following steps: after each strain is cultured for 24 hours under the optimal condition, 1-2g of thalli is obtained by centrifugation at 3000r/m, the thalli is evenly mixed with 10-20g of sterilized soil with the water content of 35-55wt% respectively, the thalli is placed in a sterile container, the sterile container is preserved for 48 hours at the temperature of 0-4 ℃, 100mL of sterile distilled water is added, 120r/m is oscillated for 20 minutes, the mixture is kept stand for 3-5 minutes, 5mL of upper liquid is sucked, the mixture is respectively inoculated in an optimal culture medium, and the culture medium is placed at the temperature of 6-8 ℃ for 24 hours, so that one cycle is adopted, and the cold-resistant domesticated strain is obtained by repeated training for 8-10 cycles. Wherein rhodopseudomonas palustris is subjected to anaerobic culture.
Optionally, the microbial agent consists of the following raw materials in parts by weight:
the cold-resistant agent consists of the following raw materials in parts by weight:
0.1-0.4 part of desulfurized gypsum;
1-4 parts of seaweed powder;
0.1-0.6 part of carotenoid;
the carotenoid is at least one of lutein powder, beta-carotene powder and lycopene powder;
the seaweed powder is at least one of kelp powder, undaria pinnatifida powder, gulfweed powder and sargassum thunbergii powder.
Optionally, the microbial agent is a mixture of strain freeze-dried powder, a strain fermentation broth mixture or fermentation broth freeze-dried powder. The microbial agent and the cold-resistant agent can form a solid microbial agent composition or a liquid microbial agent composition.
For example, in one embodiment, the microbial agent is a mixture of bacterial strains, the effective viable count of Paenibacillus mucilaginosus is greater than or equal to 4 hundred million cfu/mL, the effective viable count of Rhodopseudomonas palustris is greater than or equal to 4 hundred million cfu/mL, the effective viable count of Bacillus subtilis is greater than or equal to 4 hundred million cfu/mL, the effective viable count of Bacillus megaterium is greater than or equal to 4 hundred million cfu/mL, and the effective viable count of Paenibacillus polymyxa is greater than or equal to 4 hundred million cfu/mL.
Optionally, the raw materials comprise, by weight:
50-100 parts of microbial agent;
2-4 parts of cold resistant agent.
Optionally, the preparation method comprises the following steps of:
50-100 parts of microbial agent;
2-4 parts of a cold resistant agent;
0.5-2 parts of fulvic acid.
Fulvic acid has the following effects: firstly, the effect of regulating the growth of crops and improving the resistance of the crops has remarkable effects on stress resistance and quality improvement and yield increase of the crops; secondly, the fulvic acid can promote substances in the cold-resistant agent to be absorbed and utilized by crops, and the fulvic acid solution is acidic, so that the release of calcium ions can be accelerated, and the solubility of the calcium ions in the desulfurized gypsum in the cold-resistant agent is improved; the dissolution and release of active ingredients in the seaweed powder are promoted, and the absorption of crops to cold resistant agents is promoted; thirdly, the fulvic acid is rich in nutrients such as organic matters, so that the soil fertility can be enhanced, and the crop growth can be promoted; fourth, the fulvic acid can improve the activity of the root system of the crops, improve the activity of enzymes in the crops, and promote the improvement of stress resistance of the crops.
Optionally, the preparation method comprises the following steps of:
the inorganic fertilizer is at least one of potash fertilizer, phosphate fertilizer, nitrogenous fertilizer and silicon fertilizer.
Optionally, the potash fertilizer is at least one of potassium nitrate, potassium chloride and potassium sulfate.
Wherein the purity of the potassium nitrate is 98%, K 2 The O content is 45.6 percent, and the N content is 13.6 percent; the purity of the potassium chloride is 95%, K 2 The O content is 59.9%; the purity of the potassium sulfate is 98%, K 2 The O content was 52.9%.
Optionally, the phosphate fertilizer is monoammonium phosphate, the purity is 99%, and P 2 O 5 The content is 61.1% and the N content is 12%.
Optionally, the nitrogen fertilizer is at least one of urea and ammonium nitrate. Wherein, the purity of the urea is 99 percent, and the N content is 46.2 percent; the purity of ammonium nitrate is 98%, and the N content is 34.3%.
Optionally, the silicon fertilizer is solid powder with purity of 99%, wherein SiO 2 The content is more than or equal to 50 percent, and the content of effective silicon is 23.3 percent. For the rice of the silicon-like crops, the content of effective silicon in soil is increased, and the method has better enhancement effects on the activity of the root system of the rice, photosynthesis, nutrient supply and stress resistance of the rice, so that the yield of the rice is increased and the quality of the rice is improved.
Optionally, the raw materials comprise, by weight:
the microelements are derived from at least one of EDTA chelated zinc powder, EDTA chelated manganese powder and tetra-water disodium octaborate powder.
Optionally, the EDTA chelated zinc powder has a purity of 98% and a zinc content of 14.5%; the purity of the EDTA chelated manganese powder is 99%, and the manganese content is 16%; the purity of the disodium octaborate tetrahydrate powder is 99%, and the boron content is 21%.
Optionally, the microbial agent composition comprises the following raw materials in parts by weight:
the defoamer is polyether modified organic silicon defoamer. In the process of preparing the liquid microbial agent composition, a large amount of foam is often generated, and the generation of the foam can cause the overflow of the reaction liquid to waste raw materials, reduce the uniformity of the aqueous dispersion of the product, influence the use effect of the product and pollute the surrounding environment, so that the problem of foaming is solved by adding an antifoaming agent.
The application also provides a preparation method of the microbial agent composition, and the components are mixed according to the weight part ratio to form a mixture.
Optionally, when the raw materials contain fulvic acid, the fulvic acid is mixed with the strain fermentation broth, and then the cold-resistant agent, the inorganic fertilizer and the defoaming agent are added for mixing. The operation can lead the solubility of the desulfurization gypsum in the cold-resistant agent in the acid solution to be higher than that of water, improve the utilization rate of algal polysaccharide in the seaweed meal under the acid condition, and simultaneously lead the activity of the silicon fertilizer to be stronger under the acid condition and increase the content of effective silicon.
The application also provides application of the microbial agent composition in crop cultivation. The microbial agent composition is applied before or during the cultivation of crops, so that the use of chemical fertilizers can be reduced, nutrition can be provided for the growth of crops, and the cold resistance and cold resistance of the crops can be improved, so that the crops can still grow normally under the condition of low temperature.
For example, in one embodiment, the crop is rice, the minimum temperature for seedling cultivation is 4-12 ℃, and the field growth temperature is 4-38 ℃.
Compared with the prior art, the application has at least the following technical effects:
(1) The microbial agent composition provided by the application comprises the microbial agent and the cold-resistant agent with synergistic effect, and is applied to crop cultivation, the microbial agent can improve the microbial population structure of soil, promote the release of nutrient components in the cold-resistant agent, inorganic fertilizer and the like so as to be conveniently utilized by crops, and the cold-resistant agent can be used as the nutrient components to be absorbed by the crops on one hand, and can promote the synthesis of cold-resistant hormone which can be absorbed by the crops by microorganisms on the other hand, so that the cold resistance of the crops is further improved, and the crops can better resist low-temperature environments and grow normally.
(2) The microbial agent composition can effectively decompose indissolvable substances such as phosphorus, potassium, minerals and the like in soil, loosen the soil, improve the soil aggregate structure, enhance the physiological performance of the soil, protect crop roots from invasion of pathogenic microorganisms and play a role in alleviating crop diseases.
Detailed Description
The technical scheme of the application is further described below with reference to the specific embodiments, but the application is not limited thereto.
Example 1
(1) Uniformly mixing 15kg of a bacillus mucilaginosus fermentation broth, 8kg of a rhodopseudomonas palustris fermentation broth, 20kg of a bacillus subtilis fermentation broth, 20kg of a bacillus megatherium fermentation broth and 15kg of a paenibacillus polymyxa fermentation broth to obtain 78kg of a strain fermentation broth mixture;
(2) Uniformly mixing 0.4kg of desulfurized gypsum, 0.5kg of kelp powder, 0.5kg of undaria pinnatifida powder, 0.5kg of gulfweed powder, 0.5kg of sargassum thunbergii powder, 0.3kg of lutein powder, 0.2kg of beta-carotene and 0.1kg of lycopene powder to obtain 3.0kg of cold resistant agent;
(3) Uniformly mixing 78kg of the strain fermentation liquor mixture and 3.0kg of the cold-resistant agent to prepare a microbial agent composition A;
(4) The effective viable count, the mixed bacterial rate and the pH of the microbial agent composition A were measured, and the results are shown in Table 1.
Example 2
(1) Fully and uniformly mixing 15kg of bacillus mucilaginosus fermentation broth, 8kg of rhodopseudomonas palustris fermentation broth, 20kg of bacillus subtilis fermentation broth, 20kg of bacillus megaterium fermentation broth and 15kg of paenibacillus polymyxa fermentation broth to obtain 78kg of strain fermentation broth mixture;
(2) Uniformly mixing 0.4kg of desulfurized gypsum, 0.5kg of kelp powder, 0.5kg of undaria pinnatifida powder, 0.5kg of gulfweed powder, 0.5kg of sargassum thunbergii powder, 0.3kg of lutein powder, 0.2kg of beta-carotene and 0.1kg of lycopene powder to obtain 3.0kg of cold resistant agent;
(3) Sequentially adding 1kg of fulvic acid and 3.0kg of cold-resistant agent into 78kg of strain fermentation liquor mixture, and uniformly mixing to obtain a microbial agent composition B;
(4) The effective viable count, the mixed bacterial rate and the pH of the microbial agent composition B were measured, and the results are shown in Table 1.
Example 3
(1) Uniformly mixing 15kg of a bacillus mucilaginosus fermentation broth, 8kg of a rhodopseudomonas palustris fermentation broth, 20kg of a bacillus subtilis fermentation broth, 20kg of a bacillus megatherium fermentation broth and 15kg of a paenibacillus polymyxa fermentation broth to obtain 78kg of a strain fermentation broth mixture;
(2) Uniformly mixing 0.4kg of desulfurized gypsum, 0.5kg of kelp powder, 0.5kg of undaria pinnatifida powder, 0.5kg of gulfweed powder, 0.5kg of sargassum thunbergii powder, 0.3kg of lutein powder, 0.2kg of beta-carotene and 0.1kg of lycopene powder to obtain 3.0kg of cold resistant agent;
(3) Uniformly mixing 1.5kg of potassium chloride, 0.2kg of potassium nitrate, 0.1kg of potassium sulfate, 1kg of urea, 0.3kg of ammonium nitrate, 1.1kg of monoammonium phosphate and 0.6kg of silicon fertilizer to obtain 4.8kg of inorganic fertilizer;
(4) Uniformly mixing 0.2kg of EDTA chelated zinc powder, 0.1kg of EDTA chelated manganese powder and 0.05kg of disodium octaborate tetrahydrate to obtain 0.35kg of trace elements;
(5) Uniformly mixing 1kg of fulvic acid with 78kg of strain fermentation liquor mixture, and then adding 3.0kg of cold-resistant agent, 4.8kg of inorganic fertilizer, 0.35kg of trace elements and 1kg of defoamer to prepare a microbial agent composition C;
(6) The effective viable count, the mixed bacterial rate and the pH of the microbial agent composition C were measured, and the results are shown in Table 1.
Example 4
(1) Uniformly mixing 10kg of a bacillus mucilaginosus fermentation broth, 5kg of a rhodopseudomonas palustris fermentation broth, 10kg of a bacillus subtilis fermentation broth, 10kg of a bacillus megatherium fermentation broth and 10kg of a paenibacillus polymyxa fermentation broth to obtain 45kg of a strain fermentation broth mixture;
(2) Uniformly mixing 0.8kg of kelp powder, 0.2kg of undaria pinnatifida powder, 0.8kg of gulfweed powder and 0.2kg of sargassum thunbergii powder to obtain 2kg of cold-resistant agent;
(3) Uniformly mixing 1.0kg of potassium nitrate, 0.5kg of potassium chloride, 0.2kg of potassium sulfate, 0.5kg of urea, 0.5kg of ammonium nitrate, 1.0kg of monoammonium phosphate and 0.5kg of silicon fertilizer to obtain 4.2kg of inorganic fertilizer;
(4) Uniformly mixing 0.12kg of EDTA chelated zinc powder, 0.08kg of EDTA chelated manganese powder and 0.05kg of disodium octaborate tetrahydrate to obtain 0.25kg of trace elements;
(5) Uniformly mixing 0.5kg of fulvic acid with 45kg of strain fermentation liquor mixture, and then adding 2.0kg of cold-resistant agent, 4.2kg of inorganic fertilizer, 0.25kg of trace elements and 0.5kg of defoamer to prepare a microbial agent composition D;
(6) The effective viable count, the mixed bacterial rate and the pH of the microbial agent composition D were measured, and the results are shown in Table 1.
Example 5
(1) Uniformly mixing 15kg of a bacillus mucilaginosus fermentation broth, 10kg of a rhodopseudomonas palustris fermentation broth, 15kg of a bacillus subtilis fermentation broth, 15kg of a bacillus megatherium fermentation broth and 15kg of a paenibacillus polymyxa fermentation broth to obtain a 70kg strain fermentation broth mixture;
(2) Uniformly mixing 0.3kg of desulfurized gypsum, 0.8kg of kelp powder, 0.5kg of undaria pinnatifida powder, 0.6kg of gulfweed powder, 0.2kg of sargassum thunbergii powder, 0.3kg of lutein powder, 0.2kg of beta-carotene and 0.1kg of lycopene powder to obtain 3.0kg of cold-resistant agent;
(3) Uniformly mixing 1.0kg of potassium nitrate, 0.1kg of urea, 0.7kg of ammonium nitrate, 0.7kg of monoammonium phosphate and 0.6kg of silicon fertilizer to obtain 3.1kg of inorganic fertilizer;
(4) Uniformly mixing 0.06kg of EDTA chelated zinc powder, 0.08kg of EDTA chelated manganese powder and 0.05kg of disodium octaborate tetrahydrate to obtain 0.19kg of trace elements;
(5) Uniformly mixing 0.8kg of fulvic acid with 70kg of strain fermentation liquor mixture, and then adding 3.0kg of cold-resistant agent, 3.1kg of inorganic fertilizer, 0.19kg of trace elements and 0.5kg of defoamer to prepare a microbial agent composition E;
(6) The effective viable count, the mixed bacterial rate and the pH of the microbial agent composition E were measured, and the results are shown in Table 1.
Example 6
The microbial agent composition F was prepared in the same manner as in example 1 except that rhodopseudomonas palustris liquid was not added, and the number of viable bacteria, the rate of mixed bacteria and the pH were measured, and the results are shown in Table 1.
Example 7
The other components were the same as in example 2 except that kelp powder, undaria powder, gulfweed powder and sargassum thunbergii powder were not added, and a microbial agent composition G was prepared, and the number of viable bacteria, the rate of mixed bacteria and pH thereof were measured, with the results being shown in table 1.
Comparative example 1
The other components were the same as in example 3 without adding the strain broth mixture and the cold-resistant agent.
Comparative example 2
The other components were the same as in example 3 without adding the strain broth mixture.
Comparative example 3
Other components were the same as in example 3 without adding the cold-resistant agent.
TABLE 1 effective viable count, mixed bacterial rate and pH
The application effect of the microbial agent composition of the application is further illustrated by a rice seedling test and a field test.
1. Experimental basic conditions
Table 2 experimental basic conditions
2. Test rice variety
And (5) selecting the Kazakhstan rice No. 2 as an experimental rice variety for planting.
3. Experimental materials
Microbial inoculant compositions prepared in examples 1-3 and compositions prepared in comparative examples 1-3.
4. Experimental method
4.1 seedling test
Before seedling raising, respectively applying the microbial agent compositions prepared in the examples 1-3 to seedling raising soil in the seedling raising trays 1-3; 1-3 seedling raising trays are respectively applied with the compositions prepared in comparative examples 1-3, and the application amount is 0.05 kg/tray; the control seedling tray seedling soil is not applied with the composition, the same amount of water is applied, and other management is the same.
Selecting seeds with consistent maturity and 0.6% of diluted HNO 3 Dormancy breaking treatment for 16h, and then placing the mixture into a culture box at 28-30 ℃ for 40-48h to accelerate germination. The seeds with good germination acceleration are sequentially sowed into a seedling raising tray, the edge of the tray is marked with a treatment serial number by a marker pen, and then the seeds are placed into a rice light-temperature controllable growth chamber for growth under the following specific growth conditions:
culture temperature: 12 hours in daytime and 20-26 ℃; and (3) treating for 1 time at the temperature of 4-12 ℃ after 12 hours at 15-20 ℃ at night and 16-17d after emergence of seedlings at last 1 hour at night.
Photoperiod: 12 hours in daytime; night for 12 hours; ambient humidity: relative humidity (Rh) about 80%; illumination intensity: 2500-3500lux.
And (3) measuring the index of the cold resistance of the rice seedlings after 12 hours of treatment: conductivity, chlorophyll content, soluble sugar content, free proline content, superoxide dismutase (SOD) content, malondialdehyde (MDA) content, peroxidase (POX) content, and Catalase (CAT) content.
4.2 field test
The test is carried out in Halbine city incense house area Happy town experimental paddy field (the temperature is 4-38 ℃ in 5-10 months), the paddy field is divided into 7 areas, the length of each area is 6m, the width of each area is 3m, wherein 6 areas are test areas, and 1 area is a control area. The 6 areas are respectively marked as an experimental area 1, an experimental area 2, an experimental area 3, a comparison area 1, a comparison area 2 and a comparison area 3, 7d before rice transplanting is carried out, and the microbial agent compositions prepared in the examples 1-3 are respectively applied to the experimental areas 1-3; the compositions prepared in comparative examples 1 to 3 are respectively applied to the comparative areas 1 to 3, and the application amount is 25 kg/mu; the control zone was not applied with the composition and was otherwise managed as the experimental zone, and was performed conventionally.
After the rice is mature, various indexes of the rice in each region are measured, including plant height, spike length, spike number, seed setting rate, thousand seed weight and yield.
5. Experimental results
TABLE 3 influence of microbial inoculant compositions on Cold resistance of Rice seedlings
As can be seen from Table 3, compared with the comparison area and the control area, after the microbial agent compositions prepared in the embodiments 1-3 of the application are respectively applied in the experimental areas 1-3, the increase of the conductivity of rice seedlings under low temperature stress can be slowed down, the integrity of cell membranes is maintained, and the extravasation of electrolyte is reduced; the contents of chlorophyll, soluble sugar, free proline and other stress-resistant substances in the rice seedlings are obviously improved, the activities of SOD, POX, CAT enzyme and other stress-resistant enzymes in the rice seedlings are improved, the antioxidant system of cells is activated, the free radical removal capability of the rice seedlings is improved, the balance of free radical generation and removal of the rice seedlings is maintained, and therefore the cells are prevented from being damaged by the free radicals; the MDA content is reduced, the damage degree of the rice seedling cell lipid is reduced, and the capability of the cell to remove active oxygen is improved, so that the cold resistance of the rice seedling is improved, and the damage of cold to the rice seedling is reduced. The cold-resistant duration of the microbial agent composition provided by the application is as long as 29-46 days, and the main defects of unobvious cold-resistant effect, short duration and the like in the prior art can be overcome.
Table 4 experimental results of rice planting conditions
As shown in Table 4, after the microbial agent composition of the application is applied, the plant height, the spike length, the spike grain number, the fruiting rate, the thousand grain weight and the yield of the rice are all obviously higher than those of a comparison area and a comparison area, the yield can be increased by 9.7% as compared with the comparison area, and the yield can be increased by 12.6% as compared with the comparison area. This demonstrates that the yield and quality of rice can be improved by applying the microbial inoculant composition of the present application.
The above description is only a preferred embodiment of the present application, and the patent protection scope of the present application is defined by the claims, and all equivalent structural changes made in the content of the present application are included in the protection scope of the present application.

Claims (5)

1. The preparation method of the microbial agent composition for rice cultivation is characterized by comprising the following steps of mixing the following raw materials in parts by weight to form a mixture:
50-100 parts of microbial agent;
2-4 parts of a cold resistant agent;
0.5-2 parts of fulvic acid;
the microbial agent is a strain fermentation liquor mixture, and when the microbial agent is mixed, the fulvic acid and the strain fermentation liquor mixture are mixed, and then other components are added;
the microbial agent consists of the following raw materials in parts by weight:
the cold-resistant agent consists of the following raw materials in parts by weight:
0.1-0.4 part of desulfurized gypsum;
1-4 parts of seaweed powder;
0.1-0.6 part of carotenoid;
the carotenoid is at least one of lutein powder, beta-carotene powder and lycopene powder;
the seaweed powder is at least one of kelp powder, undaria pinnatifida powder, gulfweed powder and sargassum thunbergii powder.
2. The preparation method according to claim 1, wherein in the strain fermentation broth mixture, the effective viable count of the paenibacillus mucilaginosus is more than or equal to 4 hundred million cfu/mL, the effective viable count of rhodopseudomonas palustris is more than or equal to 4 hundred million cfu/mL, the effective viable count of bacillus subtilis is more than or equal to 4 hundred million cfu/mL, the effective viable count of bacillus megaterium is more than or equal to 4 hundred million cfu/mL, and the effective viable count of paenibacillus polymyxa is more than or equal to 4 hundred million cfu/mL.
3. The preparation method according to claim 1, characterized by comprising, in parts by weight:
the inorganic fertilizer is at least one of potash fertilizer, phosphate fertilizer, nitrogenous fertilizer and silicon fertilizer.
4. The preparation method according to claim 3, wherein the raw materials comprise, in parts by weight:
the microelements are derived from at least one of EDTA chelated zinc powder, EDTA chelated manganese powder and tetra-water disodium octaborate powder.
5. The preparation method according to claim 4, wherein the microbial agent composition comprises the following raw materials in parts by weight:
the defoamer is polyether modified organic silicon defoamer.
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