CN115322930B - Soil probiotics for promoting tobacco seedling growth - Google Patents
Soil probiotics for promoting tobacco seedling growth Download PDFInfo
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- CN115322930B CN115322930B CN202210999079.8A CN202210999079A CN115322930B CN 115322930 B CN115322930 B CN 115322930B CN 202210999079 A CN202210999079 A CN 202210999079A CN 115322930 B CN115322930 B CN 115322930B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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Abstract
The invention discloses soil probiotics for promoting tobacco seedling growth, belonging to the technical field of microorganisms, and having the strain name:Bacillus zanthoxyli ZY2, deposit unit: china center for type culture Collection, date of preservation: 2021, 7 and 19, deposit number: cctccc No. M2021897. Through experiments and measurements, the soil probiotics for promoting tobacco seedling growth can promote tobacco seedling growth in the small cross period and the large cross period of flue-cured tobacco respectively, and especially has remarkable improvement on root growth and root activity. In addition, after the microbial inoculum is applied, the activity of catalase and the content of soluble protein are obviously increased, which indicates that the stress resistance of tobacco seedlings is enhanced.
Description
Technical Field
The invention relates to the technical field of microorganisms, in particular to soil probiotics for promoting tobacco seedling growth.
Background
Tobacco is a plant of the genus nicotiana of the family Solanaceae, usually annual herbs, and the main value of tobacco is to make flue-cured tobacco from its leaves and stems and then process the tobacco into cigarettes. Tobacco is cultivated in each province in China, and is an important economic crop.
The root system is an important guarantee of the health and high yield of the overground parts of plants (tobacco). Substances necessary for plant growth such as nutrients and moisture are mainly absorbed by the root system, so that methods for improving the absorption of the nutrients and moisture by the root system are always sought, and the methods mainly comprise the steps of expanding the area of the root system, enhancing the activity of the root system, such as soil loosening and fertilization for creating a good soil environment for the growth of the root system, adding plant growth regulators for promoting the activity of the root system, and focusing research hotspots on soil probiotics in recent years.
The main functions of the soil probiotics are promoting the release of elements such as nitrogen, phosphorus and potassium, stimulating the growth of crops, killing insects and bacteria, increasing the resistance of crops and the like, and the common types at present are bacillus subtilis, bacillus megaterium, bacillus thuringiensis, bacillus licheniformis, bacillus laterosporus, bacillus mucilaginosus, streptomyces jingyang, azotobacter brown, mycorrhizal fungi, lactobacillus and the like. The screening, function definition and applicable environment research of the soil beneficial bacteria are research hot spots of agricultural production all the time since the last 80 th century.
Microorganisms have a certain selectivity to the ecological environment, and although a large number of soil-beneficial microorganism preparations or fertilizers are appeared on the market, the effect can be shown to be good only in a limited area, so that the popularization of beneficial microorganisms on a large scale is difficult.
Disclosure of Invention
The invention aims to provide soil probiotics for promoting tobacco seedling growth, and the strain names are as follows:Bacillus zanthoxyliZY2, deposit unit: china center for type culture Collection, date of preservation: 2021, 7 and 19, deposit number: cctccc No. M2021897.
Through experiments and measurements, the soil probiotics for promoting tobacco seedling growth can promote tobacco seedling growth in the small cross period and the large cross period of flue-cured tobacco respectively, and especially has remarkable improvement on root growth and root activity. In addition, after the microbial inoculum is applied, the activity of catalase and the content of soluble protein are obviously increased, which indicates that the stress resistance of tobacco seedlings is enhanced.
Drawings
FIG. 1 is a photograph of the growth status of tobacco seedlings of 7d applied to a tobacco cross-stage agent in examples of soil probiotics for promoting tobacco seedling growth according to the present invention.
Fig. 2 is a photograph of root growth of tobacco seedlings after 7d application of soil probiotics for tobacco macrocross in an example.
FIG. 3 is a comparison of root activity of 7d flue-cured tobacco seedlings applied to a tobacco macrocross inoculant in examples by soil probiotics for promoting tobacco seedling growth in accordance with the present invention.
Fig. 4 is a comparison of catalase activity of tobacco seedlings in 7d applied to a tobacco macrocross agent in examples by soil probiotics promoting tobacco seedling growth according to the present invention.
Fig. 5 is a comparison of soluble protein content of tobacco seedlings for 7d applied to a tobacco macrocross agent in examples of soil probiotics for promoting tobacco seedling growth according to the present invention.
FIG. 6 is a photograph showing the growth of tobacco seedlings in the seedling stage after 7d microbial inoculum application to tobacco macrocross microbial inoculum in examples of soil probiotics for promoting tobacco seedling growth according to the invention.
Detailed Description
The technical scheme of the present invention will be clearly and completely described in the following in connection with the specific embodiments of the present invention. The described embodiments are only some, but not all, embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The invention relates to soil probiotics for promoting tobacco seedling growth, which has the following strain names:Bacillus zanthoxyliZY2, deposit unit: china center for type culture Collection, date of preservation: 2021, 7 and 19, deposit number: cctccc No. M2021897.
Design of experiments
Using a single sheetThe factor test is carried out by respectively applying ZY2 microbial inoculum treatment to small cross-stage and large cross-stage tobacco seedlings, and repeating for 3 times by taking no microbial inoculum as a Control (CK). Wherein the viable count of the ZY2 microbial inoculum is more than or equal to 100 hundred million g -1 。
The seedling raising trays in the floating seedling raising pool are separated by white plastic cloth and divided into 6 cells, and 3 polystyrene floating trays of 160 Kong Penghua are placed in each cell, wherein the specification is 57 cm multiplied by 36 cm multiplied by 6 cm.
Diluting ZY2 microbial inoculum to 10 of original microbial inoculum concentration -1 The multiple (about 5 ml per plant of tobacco seedling fungicide) is added into the seedling culture matrix. The growth and development of tobacco seedlings are observed every day after the microbial inoculum is applied, and the growth vigor of the tobacco seedlings is recorded. A representative tobacco seedling 15 plant is selected in each cell, the fresh weight, dry weight, root cap ratio, plant height, stem circumference, maximum leaf length, maximum leaf width, maximum leaf area, leaf number, chlorophyll content of the first leaf/the second leaf and main indexes of root system such as the longest root length, root dry/fresh weight, root surface area, root volume and root system activity of the whole plant of the tobacco seedling are recorded, and the measurement of the physiological indexes of the root system of the tobacco seedling mainly comprises Catalase (CAT) activity and soluble protein content (SP).
Measurement method
2.1 determination of agronomic Properties and root System index of tobacco seedlings
After 7d of application of the microbial inoculum, photographing recording, sampling and measuring are carried out. Wherein, the agronomic traits of tobacco seedlings are measured by referring to Chinese tobacco industry standard YC/T142-2010; root surface area and root volume were measured using a root scanner (model: epson expression 1000 xl); chlorophyll is measured by a chlorophyll meter; root system viability was determined using triphenyltetrazolium chloride reduction method (TTC method).
Determination of resistance-related indicators
Measurement results
Screening out a strain of bacteria Bacillus zanthoxyli (bacillus Zanthoxylum) in Guizhou Zunyi tobacco planting soil, and applying the bacteria in a small cross period and a large cross period of flue-cured tobacco respectively can promote tobacco seedling growth (shown in fig. 1, table 1 and fig. 6), and especially obviously improve root growth (shown in table 1 and fig. 2) and root activity (shown in fig. 3). In addition, the catalase activity (figure 4) and the soluble protein content (figure 5) are obviously increased after the microbial inoculum is applied, which indicates that the stress resistance of the tobacco seedlings is enhanced.
Fig. 1 is a photograph of the growth status of tobacco seedlings of 7d applied to a tobacco cross-stage microbial inoculum in an example of soil probiotics for promoting tobacco seedling growth according to the invention.
Fig. 2 is a photograph showing the root growth of tobacco seedlings after 7d application of the soil probiotics for promoting tobacco seedling growth in the example.
FIG. 3 is a comparison of root activity of 7d flue-cured tobacco seedlings applied to a tobacco macrocross inoculant in examples by soil probiotics for promoting tobacco seedling growth in accordance with the present invention.
Fig. 4 is a graph showing the comparison of catalase activity of tobacco seedlings in 7d applied to the tobacco macrocross agent in examples.
Fig. 5 is a comparison of soluble protein content of tobacco seedlings for 7 days applied to the tobacco macrocross inoculant in the examples by the soil probiotics for promoting tobacco seedling growth according to the present invention.
FIG. 6 is a photograph showing the growth status of tobacco seedlings in the seedling stage after 7d microbial inoculum application to tobacco macrocross microbial inoculum in examples of soil probiotics for promoting tobacco seedling growth according to the invention.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present disclosure describes embodiments, not every embodiment is provided with a separate embodiment, and that this description is provided for clarity only, and that the disclosure is not limited to the embodiments described in detail below, and that the embodiments described in the examples may be combined as appropriate to form other embodiments that will be apparent to those skilled in the art.
Claims (1)
1. Soil probiotics for promoting tobacco seedling growth and strain names:Bacillus zanthoxyliZY2, deposit unit: china center for type culture Collection, date of preservation: 2021, 7 and 19, deposit number: cctccc No. M2021897.
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| CN115322930A CN115322930A (en) | 2022-11-11 |
| CN115322930B true CN115322930B (en) | 2023-06-09 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103087944A (en) * | 2012-12-07 | 2013-05-08 | 贵州省烟草公司毕节市公司 | Bacilluscereus and application thereof |
| CN109997450A (en) * | 2019-04-04 | 2019-07-12 | 青岛农业大学 | A kind of production method promoting cigarette quality using addition probiotics |
| CN113201474A (en) * | 2021-04-26 | 2021-08-03 | 中国农业科学院烟草研究所 | Bacillus subtilis TBWR1, application thereof and obtained control agent |
| KR20220067077A (en) * | 2020-11-17 | 2022-05-24 | 동아대학교 산학협력단 | Bacillus zanthoxyli strain promoting tolerance of plants and use thereof |
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- 2022-08-19 CN CN202210999079.8A patent/CN115322930B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103087944A (en) * | 2012-12-07 | 2013-05-08 | 贵州省烟草公司毕节市公司 | Bacilluscereus and application thereof |
| CN109997450A (en) * | 2019-04-04 | 2019-07-12 | 青岛农业大学 | A kind of production method promoting cigarette quality using addition probiotics |
| KR20220067077A (en) * | 2020-11-17 | 2022-05-24 | 동아대학교 산학협력단 | Bacillus zanthoxyli strain promoting tolerance of plants and use thereof |
| CN113201474A (en) * | 2021-04-26 | 2021-08-03 | 中国农业科学院烟草研究所 | Bacillus subtilis TBWR1, application thereof and obtained control agent |
Non-Patent Citations (2)
| Title |
|---|
| "The Bacillus zc}nthoxyli HS1 Strain Renders Vegetable Plants Resistant and Tolerant against Pathogen Infection and High Salinity Stress";Alisher Usmonov et al.;《The Plant Pathology Journal》;第37卷(第1期);第72-78页 * |
| "内生枯草芽孢杆菌B-001对烟草幼苗的 促生作用及其生长动态";易有金等;《植物保护学报》;第34卷(第6期);第619-623页 * |
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