CN110260976B - Method for obtaining applied dose of microorganism AM and DSE fungus combined microbial inoculum by thermal infrared monitoring - Google Patents
Method for obtaining applied dose of microorganism AM and DSE fungus combined microbial inoculum by thermal infrared monitoring Download PDFInfo
- Publication number
- CN110260976B CN110260976B CN201910505650.4A CN201910505650A CN110260976B CN 110260976 B CN110260976 B CN 110260976B CN 201910505650 A CN201910505650 A CN 201910505650A CN 110260976 B CN110260976 B CN 110260976B
- Authority
- CN
- China
- Prior art keywords
- plant
- alternaria
- canopy
- temperature
- corn
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241000233866 Fungi Species 0.000 title claims abstract description 41
- 238000000034 method Methods 0.000 title claims abstract description 25
- 239000002068 microbial inoculum Substances 0.000 title abstract description 10
- 238000012544 monitoring process Methods 0.000 title abstract description 7
- 244000005700 microbiome Species 0.000 title description 6
- 241000196324 Embryophyta Species 0.000 claims abstract description 88
- 230000000813 microbial effect Effects 0.000 claims abstract description 64
- 230000008635 plant growth Effects 0.000 claims abstract description 11
- 230000001737 promoting effect Effects 0.000 claims abstract description 11
- 230000000694 effects Effects 0.000 claims abstract description 10
- 238000012258 culturing Methods 0.000 claims abstract description 6
- 238000009331 sowing Methods 0.000 claims abstract description 5
- 240000008042 Zea mays Species 0.000 claims description 72
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 70
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 65
- 235000005822 corn Nutrition 0.000 claims description 65
- 241000223600 Alternaria Species 0.000 claims description 27
- 241001558165 Alternaria sp. Species 0.000 claims description 25
- 230000012010 growth Effects 0.000 claims description 15
- 241000156811 Glomus versiforme Species 0.000 claims description 11
- 241000235503 Glomus Species 0.000 claims description 9
- 238000004321 preservation Methods 0.000 claims description 8
- 235000007164 Oryza sativa Nutrition 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 235000009566 rice Nutrition 0.000 claims description 6
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims description 5
- 235000009973 maize Nutrition 0.000 claims description 5
- 238000009629 microbiological culture Methods 0.000 claims description 5
- 230000001105 regulatory effect Effects 0.000 claims description 5
- 238000004519 manufacturing process Methods 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 241000159512 Geotrichum Species 0.000 claims description 2
- 241000896533 Gliocladium Species 0.000 claims description 2
- 240000007594 Oryza sativa Species 0.000 claims 1
- 239000003795 chemical substances by application Substances 0.000 abstract description 51
- 238000002474 experimental method Methods 0.000 abstract description 3
- 238000012360 testing method Methods 0.000 description 23
- 239000002689 soil Substances 0.000 description 16
- 239000000243 solution Substances 0.000 description 14
- 239000002028 Biomass Substances 0.000 description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 13
- 239000000047 product Substances 0.000 description 10
- 239000001963 growth medium Substances 0.000 description 8
- 230000001954 sterilising effect Effects 0.000 description 8
- 230000001580 bacterial effect Effects 0.000 description 7
- 238000011160 research Methods 0.000 description 7
- 241000461774 Gloeosporium Species 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 6
- 239000008223 sterile water Substances 0.000 description 6
- 241000209094 Oryza Species 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 108020004414 DNA Proteins 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 238000009630 liquid culture Methods 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 239000007836 KH2PO4 Substances 0.000 description 3
- 238000012408 PCR amplification Methods 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 239000004927 clay Substances 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 3
- 229910052698 phosphorus Inorganic materials 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 238000002791 soaking Methods 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- WEEMDRWIKYCTQM-UHFFFAOYSA-N 2,6-dimethoxybenzenecarbothioamide Chemical compound COC1=CC=CC(OC)=C1C(N)=S WEEMDRWIKYCTQM-UHFFFAOYSA-N 0.000 description 2
- 241000223602 Alternaria alternata Species 0.000 description 2
- 208000035240 Disease Resistance Diseases 0.000 description 2
- 241001057636 Dracaena deremensis Species 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000005708 Sodium hypochlorite Substances 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000002411 adverse Effects 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 2
- 229960000723 ampicillin Drugs 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 238000003331 infrared imaging Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000005065 mining Methods 0.000 description 2
- 230000008520 organization Effects 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 230000001863 plant nutrition Effects 0.000 description 2
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 229960002385 streptomycin sulfate Drugs 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- XINQFOMFQFGGCQ-UHFFFAOYSA-L (2-dodecoxy-2-oxoethyl)-[6-[(2-dodecoxy-2-oxoethyl)-dimethylazaniumyl]hexyl]-dimethylazanium;dichloride Chemical compound [Cl-].[Cl-].CCCCCCCCCCCCOC(=O)C[N+](C)(C)CCCCCC[N+](C)(C)CC(=O)OCCCCCCCCCCCC XINQFOMFQFGGCQ-UHFFFAOYSA-L 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241000219317 Amaranthaceae Species 0.000 description 1
- 241000219193 Brassicaceae Species 0.000 description 1
- 208000003643 Callosities Diseases 0.000 description 1
- 241000234646 Cyperaceae Species 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 240000007171 Imperata cylindrica Species 0.000 description 1
- 108091023242 Internal transcribed spacer Proteins 0.000 description 1
- 229910021578 Iron(III) chloride Inorganic materials 0.000 description 1
- 241000209510 Liliopsida Species 0.000 description 1
- 241000218922 Magnoliophyta Species 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 description 1
- 230000036579 abiotic stress Effects 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 238000005452 bending Methods 0.000 description 1
- 230000004790 biotic stress Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 229910000388 diammonium phosphate Inorganic materials 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 210000001723 extracellular space Anatomy 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 230000000855 fungicidal effect Effects 0.000 description 1
- 239000000417 fungicide Substances 0.000 description 1
- 238000012252 genetic analysis Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 238000013081 phylogenetic analysis Methods 0.000 description 1
- 230000004962 physiological condition Effects 0.000 description 1
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Inorganic materials [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 238000012113 quantitative test Methods 0.000 description 1
- 230000010496 root system development Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 230000028070 sporulation Effects 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 235000019722 synbiotics Nutrition 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J5/00—Radiation pyrometry, e.g. infrared or optical thermometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J5/00—Radiation pyrometry, e.g. infrared or optical thermometry
- G01J2005/0077—Imaging
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J5/00—Radiation pyrometry, e.g. infrared or optical thermometry
- G01J2005/0092—Temperature by averaging, e.g. by scan
Abstract
The invention discloses a method for acquiring the applied dose of a microbial AM and DSE fungus combined microbial inoculum by thermal infrared monitoring. The method sequentially comprises the following steps: (1) sowing a plurality of plant seeds and applying microbial agents with different dosages; (2) culturing, namely measuring the temperature of a canopy 40 days after the plant seedlings emerge by using a thermal infrared imager, and determining the application dose of the microbial agent according to the temperature of the canopy; the lower the temperature of the canopy, the better the effect of the microbial agent in promoting plant growth. Experiments prove that the application dosage of the microbial agent can be obtained by adopting the method provided by the invention. The invention has important application value.
Description
Technical Field
The invention belongs to the field of microorganisms, and particularly relates to a method for acquiring the applied dose of a microbial AM and DSE (arbuscular mycorrhizal fungi) combined microbial inoculum by utilizing thermal infrared monitoring.
Background
Arbuscular Mycorrhizal Fungi (AMF) are soil microorganisms commonly existing in nature, and consist of mycorrhizal spores (fruits), arbuscular branches, vesicles and hyphae, and are infected after contacting with root systems to form a reciprocal symbiont. More than 90% of the flowering plants on land are able to form mycorrhiza symbiota with AMF fungi. The surface of the AMF fungi has a plurality of epitaxial hyphae which grow in the plant rhizosphere soil to form an extra-root hypha screen, so that the absorption area of plant roots is enlarged, the soil outside a phosphorus deficiency area in the rhizosphere range can absorb nutrients, the absorption capacity of host plants on phosphorus, mineral elements and water is effectively enhanced, the growth and development of plants are promoted, and the yield and the biomass of the plants are improved; the damage of extreme environment to plants can be reduced, the drought resistance, the cold resistance and the saline-alkali resistance of the plants can be improved, the damage of plant diseases and insect pests to the plants can be reduced, the disease resistance and the enzyme activity of the plants can be improved, the survival rate of the plants can be improved, the plant community can be recovered, and the like.
Dark Septate Endophytes (DSE) are the main group of plant endophytes and are mainly characterized in that hyphae are darker in color and have obvious membranes, and the hyphae are planted in the epidermis and cortex of a healthy plant root system and even in cells or intercellular spaces of fiber bundle tissues to form symbionts without causing plant diseases. DSE can survive in adverse and extreme environments such as high mountains, polar regions, low pH soil and the like. According to statistics, the host range of the DSE covers nearly 600 plants of 320 genus of 114 family, and the DSE is found in the roots of mycorrhizal plants and traditional non-mycorrhizal plants of cyperaceae family, cruciferae family, chenopodiaceae family and the like. DSE can promote plant growth and raise the resistance and disease resistance of the host plant. DSE can increase the photosynthetic effect of host plants, promotes plant root system development to improve the utilization ratio of plant to moisture in the soil, the root system can transport more moisture to the overground part of plant, keeps the water potential balance. The research on DSE fungi mainly focuses on the culture method of the fungi and plant bacterins and the research on the growth promotion and adverse resistance effects of the fungi on host plants.
The thermal infrared imager is an electronic detection device which detects infrared energy of an object in a non-contact mode, converts the infrared energy into an electric signal, generates a thermal image on a display and can process the thermal image. The thermal infrared imaging technology is a technology for monitoring the thermal reaction characteristics of an object by utilizing a thermal infrared imager. In recent years, the thermal infrared imaging technology is applied more in precision agriculture, and can effectively guide production practice. The chemical substances generated by the opening and closing of plant stomata and stress have large influence on the plant temperature, which is a precondition for monitoring the plant temperature by applying thermal infrared. The crop growth process is greatly influenced by environment and climate, the crop growth process indirectly reacts on the quality and yield of agricultural products, the surface temperature change and the thermal reaction characteristics of the crops can be rapidly and accurately measured by using a thermal infrared technology, the environmental factors are synchronously monitored, the growth condition of the crops is monitored in real time, the influence of the environmental factors on the growth physiological indexes of the crops is explored, and judgment and early warning are made on whether the physiological condition of the crops is subjected to abiotic or biotic stress such as drought, high temperature, salt and alkali, cold tide, plant diseases and insect pests and the like.
Disclosure of Invention
The invention aims to obtain the application dosage of the microbial agent with the function of promoting the growth of plants.
The invention firstly protects a method for obtaining the applied dosage of a microbial agent, which comprises the following steps:
(1) sowing a plurality of plant seeds and applying microbial agents with different dosages;
(2) and (3) after the step (1) is completed, culturing, and determining the application dose of the microbial agent according to the temperature of the canopy of the plant.
In the method, the microbial agent has a function of promoting plant growth.
In the above method, the plant seed may be a plant germinating seed.
The invention also discloses a method for preparing the microbial agent with the function of promoting the growth of plants, which comprises the following steps:
(a1) mixing the components according to different proportions to obtain corresponding microbial agents;
(a2) treating the plants with the microbial agents prepared in step (a1), respectively;
(a3) after completion of step (a2), culturing, and determining the composition of the microbial agent according to the canopy temperature of the plant.
In any of the above methods, the "determining the applied amount of the microbial agent according to the canopy temperature of the plant" or the "determining the composition of the microbial agent according to the canopy temperature of the plant" may be determined based on the criterion that the lower the canopy temperature of the plant is, the better the effect of the microbial agent on promoting the growth of the plant is.
Any of the microbial agents described above may include Alternaria (Alternaria sp.) fungus and glomus terrestris.
The active ingredient of any of the above microbial agents may be Alternaria (Alternaria sp.) fungus and glomus terreus.
Any of the above microbial agents may specifically consist of Alternaria (Alternaria sp.) fungi and glomus terrestris.
In any of the above microbial agents, the ratio of Alternaria (Alternaria sp.) fungus to glomus terreus may be (30-140) mg dry matter of Alternaria (Alternaria sp.) fungus: 1300 spores (e.g. (30-62) mg dry matter of Alternaria (Alternaria sp.) fungus: 1300 spores, (62-140) mg dry matter of Alternaria (Alternaria sp.) fungus: 1300 spores, 30mg dry matter of Alternaria (Alternaria sp.) fungus: 1300 spores, 31.1mg dry matter of Alternaria (Alternaria sp.) fungus: 1300 spores, 62mg dry matter of Alternaria (Alternaria sp.) fungus: 1300 spores, 62.2mg dry matter of Alternaria (Alternaria sp.) fungus: 1300 spores, 124.4mg dry matter of Alternaria (Alternaria sp.) fungus: 1300 spores or 140mg dry matter of Alternaria (Alternaria sp.) fungus: 1300 spores).
Any of the plants described above may be any of the following c1) to c 5): c1) a dicotyledonous plant; c2) a monocot plant; c3) a gramineous plant; c4) corn; c5) glutinous rice No. one of corn varieties.
Above, when the plant is waxy I in a maize variety, the optimal dose of microbial agent applied per maize plant may be 1300 spores of Gliocladium geotrichum and 124.4mg dry matter of Alternaria sp.
In any of the above microbial agents, the Alternaria (Alternaria sp.) fungus may be Alternaria (Alternaria sp.)001, which has a collection number of CGMCC No.17463 in the common microbiology center of the chinese committee for culture collection of microorganisms.
In any of the above microbial agents, the Glomus versicolor may be Glomus versiforme (G.V). The Glomus versiforme (G.V) can be provided by plant nutrition and resource research institute of agroforestry academy of sciences of Beijing, and the strain number is BGC NM 04B.
Any one of the above-mentioned "canopy temperature of the plant" may be the canopy temperature after 40d from the emergence of the plant. When the plant is waxy rice number one in corn variety, the temperature of the canopy after 40 days after emergence (such as 44 days after emergence and 57 days after emergence) can be used.
The temperature of any one of the canopies can be measured after an image is collected by a thermal infrared imager.
The invention also protects the application of any one of the methods, which can be at least one of b1) to b 3): b1) plant production; b2) planting plants; b3) regulating the growth of the plants.
The invention also protects the application of canopy temperature of plants, which can be at least one of d1) to d 4):
d1) obtaining the applied dosage of the microbial agent;
d2) preparing a microbial agent;
d3) regulating the growth of the plant;
d4) preparing a product for regulating plant growth.
In the above application, the "product for regulating plant growth" may be specifically a microbial agent.
In the above, the promotion of plant growth may be manifested by an increase in biomass and/or an increase in plant height. The biomass may be at least one of root dry weight, shoot dry weight, and leaf dry weight.
Biomass and plant height belong to indexes obtained in the mature period of corn. The method comprises the steps of detecting the canopy temperature of the corn after 40 days (such as 44 days after the corn seedlings and 57 days after the corn seedlings) by using a thermal infrared imager, and determining the application dosage or composition of a microbial agent according to the canopy temperature; the lower the temperature of the canopy, the better the effect of the microbial agent in promoting the growth of the corn. The method provided by the invention can be used for preparing the microbial agent and/or obtaining the application dose of the microbial agent. The invention has important application value.
Drawings
Fig. 1 is a corn thermal infrared image at 35d after corn emergence.
Fig. 2 is a region of interest selected when the software is used to process the corn thermal infrared image at 35d after corn emergence.
FIG. 3 shows the canopy temperature obtained when the thermal infrared image of corn at 35d after emergence of the corn is processed by software.
Fig. 4 is the statistics of 35d, 44d and 57d canopy temperatures after emergence of each group of corn.
FIG. 5 is a graph of the dependence of canopy temperature at 57d after emergence of each group of maize on total biomass.
FIG. 6 shows the correlation between the canopy temperature and plant height at 57d after emergence of each group of maize seedlings.
Deposit description
The strain name is as follows: alternaria alternata
Latin name: alternaria sp.
The strain number is as follows: 001
The preservation organization: china general microbiological culture Collection center
The preservation organization is abbreviated as: CGMCC (China general microbiological culture Collection center)
Address: xilu No.1 Hospital No. 3 of Beijing market facing Yang district
The preservation date is as follows: 04 month in 2019, 08 days
Registration number of the preservation center: CGMCC No.17463
Detailed Description
The following examples are given to facilitate a better understanding of the invention, but do not limit the invention.
The experimental procedures in the following examples are conventional unless otherwise specified.
The test materials used in the following examples were purchased from a conventional biochemical reagent store unless otherwise specified.
The quantitative tests in the following examples, all set up three replicates and the results averaged.
The seed of the glutinous rice one (hereinafter referred to as the glutinous rice one) in the corn variety is a product of Beijing Zhongnong's science and technology development Co., Ltd, and the certification number is 0103006-.
PDA solid medium: cutting peeled potato 200g into small pieces, adding 1.0L distilled water, and boiling for 30 min; filtering with gauze, collecting filtrate, adding glucose 20.0g and KH2PO4 3.0g、MgSO4.7H2O1.5 g, vitamin B110 μ g and agar 15.0g, adjusting pH to 6.0 and adding distilled water to volume of 1.0L, and sterilizing at 115 deg.C for 15 min.
PDA resistant plates: adding ampicillin and streptomycin sulfate into PDA solid culture medium cooled to 55 ℃ to obtain PDA resistance culture medium; in the PDA resistant culture medium, the concentration of ampicillin and streptomycin sulfate is 50 mg/L; the PDA-resistant medium was poured into a sterile petri dish (diameter 9cm) and naturally cooled to obtain a PDA-resistant plate.
MMN liquid medium: adding CaCl into appropriate amount of distilled water2 0.05g、MgSO4 0.15g、NaCl 0.025g、FeCl3 0.01g、KH2PO4 0.5g、Vitamib B1 0.0001g、(NH4)2HPO40.25g, Glucose 10g, Citric acid 0.2g and Malt extract 10g, adjusting pH to 5.5 and making volume to 1.0L with distilled water, and then sterilizing at 121 deg.C for 30 min.
Glomus versiforme (G.V) is provided by institute of plant nutrition and resource of agriculture and forestry academy of sciences of Beijing, and the strain number is BGC NM 04B. Hereinafter, Glomus versiforme (Glomus versiforme, G.V) is simply referred to as Glomus versiforme.
The thermal infrared imager in the following example is a product of philips corporation under model number FLIR T630 sc. The main parameters of the thermal infrared imager are shown in table 1.
TABLE 1 Main parameters of thermal infrared imager
In the following embodiments, the following reference specifications refer to the steps of acquiring a corn thermal infrared image by a thermal infrared imager, and specifically include the following steps:
firstly, turning on a thermal infrared imager, and entering a working mode of the thermal infrared imager after the instrument is stabilized;
recording the temperature and humidity information of the working environment of the thermal infrared imager;
setting radiance, environment temperature and humidity information in the thermal infrared imager to facilitate correction of heat map information;
aligning a lens of the thermal infrared imager to the corn canopy, properly adjusting the distance between the lens and the corn canopy, using a manual focusing or automatic focusing function to enable the corn canopy image to be clear, and pressing a shooting key;
fifthly, obtaining the next thermal infrared image of the corn canopy and repeating the third step and the fourth step.
Sixthly, after the shooting is finished, the heat map data is exported to a computer end, and image processing and analysis are carried out in thermal infrared professional data processing software (such as FLIR research IR Max software).
The procedure for obtaining the canopy temperature by processing the corn thermal infrared image with the FLIR research ir Max software in the following examples is as follows:
opening a shot corn thermal infrared image in FLIR research IR Max software;
selecting an interested area by using a rectangular frame, considering the phenomena of bending of corn leaves and withering of the tip parts of the corn leaves, selecting the middle one third part of each of two leaves at the top end of the corn leaves in the interested area, shooting the obtained corn leaves in the image, and rotating the rectangular frame to enable the rectangular frame to be selected at the middle one third part of the leaves;
calculating the average temperature of each interested area by adding a function, and then taking the average value of the average temperature of the interested areas as the canopy temperature of the corn.
Example 1 isolation, identification and preservation of Alternaria sp 001CGMCC No.17463
First, separate
1. The root samples (root system of needle cogongrass collected from north electric power winning mining area of the union of tin forest, city, autonomous inner Mongolia) were thoroughly washed with sterile water, and then soaked in 75% (v/v) aqueous ethanol solution for sterilization for 5min, and washed with sterile water for 2 times.
2. After step 1, the root sample is placed in a 10% (v/v) sodium hypochlorite aqueous solution for soaking and disinfection for 5min, and is washed by sterile water for 3 times.
3. And (3) after the step 2 is finished, absorbing the moisture on the surface of the root sample by using filter paper, and then shearing the root sample into root sections with the length of about 1 cm.
4. After completion of step 3, the root segments were placed on PDA resistant plates (2-3 root segments per plate) and incubated in the dark at 28 ℃ for 14 d. Colonies that could grow on PDA resistant plates were isolated and purified.
The screened fungus was named as dark septate endophytic fungus 001.
II, identification
1. Morphological identification
Inoculating the dark-color septate endophytic fungi 001 to a PDA resistant plate, performing dark inversion culture at 28 ℃ for 14d, and observing the colony characteristics; then, plug culture is carried out, and the conditions of mycelium and spore production are observed.
The growth state of the colonies is shown in FIG. 1. The results show that the color of the front surface of the bacterial colony is black gray, the back surface of the bacterial colony has no crack, the center of the bacterial colony is flat, the edge of the bacterial colony is round, and the bacterial colony is concentric.
The mycelium and sporulation were as follows: dark gray hypha, local enlargement, 5-25 μm spacing, chlamydospore, and spherical polymeric.
2. Molecular identification
(1) The genomic DNA of the fungus having a deep color of endophytic fungi 001 was extracted using a fungal genomic DNA extraction kit (a product of AXYGEN Co.) and used as a template, and the DNA was extracted using ITS 1: 5'-TCCGTAGGTGAACCTGCGG-3' and ITS 4: 5'-TCCTCCGCTTATTGATATGC-3' to obtain PCR amplification product.
The reaction system was 50. mu.L consisting of 5. mu.L of 10 XEx Taq buffer (product of Beijing Soilebao Tech Co., Ltd.), 2. mu.L of aqueous ITS1 solution (concentration: 10. mu. mol/L), 2. mu.L of ITS4 (concentration: 10. mu. mol/L), 2. mu.L of template, 4. mu.L of dNTP Mix (concentration: 2.5mM) and 35. mu.L of ddH2And (C) O.
The reaction conditions are as follows: 3min at 94 ℃; 94 ℃ for 30s, 55 ℃ for 30s, 72 ℃ for 90s, 28 cycles.
(2) Sequencing the PCR amplification product obtained in the step (1).
And (3) sequencing results show that the PCR amplification product obtained in the step (1) contains DNA molecules shown in a sequence 1 in a sequence table.
Submitting the nucleotide sequence shown in the sequence 1 in the sequence table to a GenBank database for Blast analysis, and determining the classification status. The phylogenetic tree was then constructed with MEGA 6 for genetic and phylogenetic analysis. The results show that the dark septate fungus 001 has the highest homology to Alternaria (Alternaria sp). Thus, the dark septate endophyte 001 was identified as Alternaria (Alternaria sp.).
III, preservation
The deep color endophytic fungus 001 separated in the first step is preserved in China general microbiological culture Collection center (CGMCC for short, address No. 3 of Xilu No.1 of Beijing Kogyo-Yang district of Beijing) in 2019 at 08.04, and the preservation number is CGMCC No. 17463. The dark septate endophytic fungus 001 is called Alternaria sp 001CGMCC No.17463, abbreviated as DSE strain.
Example 2 preparation of microbial Agents
Preparation of DSE culture solution
1. And (3) inoculating the DSE strain to a PDA solid culture medium, and carrying out dark inverted culture at 28 ℃ for 14d to obtain a DSE colony.
2. And (3) after the step 1 is finished, taking a fungus cake on the DSE colony under an aseptic condition, then placing the fungus cake on an MMN liquid culture medium, and carrying out shake culture at 28 ℃ and 170r/min for 15d to obtain DSE culture bacteria liquid. The mass of the dry matter of the DSE strain in 1mL of the DSE culture solution is 3.11 mg.
Secondly, preparation of glomus terrestris agent
And (3) taking the glomus versiforme, inoculating the glomus versiforme to the root of the corn plant with the sterilized sandy soil matrix, and performing propagation expansion to obtain the glomus versiforme fungicide. The ground sacculus mildew agent comprises spores, hypha outside the roots and infected root segments, the density of the spores is 26/g, the infection rate is 87%, and the length of the hypha is 3.12 m/g.
Preparation of microbial agent
Preparing a microbial agent A, a microbial agent B and a microbial agent C.
The microbial agent A consists of 10mL of DSE culture solution, 40mL of MMN liquid culture medium and 50g of gloeosporium terrestris. In the microbial agent A, the mass of the dry matter of the DSE strain is 31.1mg, and the number of spores of the gloeosporium incarnatum is 1300.
The microbial agent B consists of 20mL of DSE culture solution, 30mL of MMN liquid culture medium and 50g of gloeosporium terrestris culture solution. In the microbial agent B, the mass of the dry matter of the DSE strain is 62.2mg, and the number of spores of the gloeosporium incarnatum is 1300.
The microbial inoculum C consists of 40mL of DSE culture solution, 10mL of MMN liquid culture medium and 50g of gloeosporium terrestris culture solution. In the microbial preparation C, the dry matter mass of the DSE strain is 124.4mg, and the number of spores of gloeosporium incarnatum is 1300.
Examples 3 and 2 Effect of the microbial Agents on corn canopy temperature, Total Biomass and plant height
Preparation of corn sprout
Soaking and sterilizing the seeds of the medium glutinous rice I with 75% (v/v) ethanol water solution for 5min, and washing with sterile water for 3 times; then soaking and sterilizing for 10min by using a 10% (m/v) sodium hypochlorite aqueous solution, and washing for 3 times by using sterile water; and finally placing the corn sprouts in a culture dish paved with wet sterile filter paper, culturing the corn sprouts in the dark at 25 ℃ for 3d, and selecting the corn sprouts with consistent growth vigor for later use.
Preparation of DSE inactivated bacterial liquid and glomus terrestris inactivated bacterial agent
1. And (3) taking the DSE culture solution prepared in the step one in the example 2, and sterilizing at 121 ℃ for 30min to obtain the DSE inactivated solution.
2. And (3) sterilizing the sacculus terrestris microbial inoculum prepared in the second step in the embodiment 2 for 30min at 121 ℃ to obtain the sacculus terrestris inactivated microbial inoculum.
Third, the influence of the microbial inoculum prepared in example 2 on the corn canopy temperature, total biomass and plant height
1. Respectively pulverizing clay and sandy soil, sieving (mesh number of 2mm), sterilizing at 121 deg.C for 2 hr, and naturally air drying to obtain air-dried clay and air-dried sandy soil.
2. And (3) after the step 1 is finished, fully and uniformly mixing the air-dried clay and the air-dried sandy soil according to the mass ratio of 1: 1 to obtain mixed soil.
3. After the step 2 is finished, taking 16 plastic basins (the specifications of the plastic basins are 23.5cm in outer diameter, 20cm in inner diameter, 21.5cm in height and 15cm in bottom diameter), and firstly using 75 percent (v ^ 5 ^ 15cmv) wiping with ethanol water solution, washing with sterile water for several times, airing, filling into 5kg of mixed soil, simultaneously watering to 70% of the maximum water holding capacity of the soil, adding NH after 48h4NO3、KH2PO4And KNO3And (3) mixing the crystals (serving as base fertilizers) uniformly to ensure that the mass fraction of N, P, K in the soil is 100mg/kg, 25mg/kg and 150mg/kg respectively.
4. After step 3 was completed, the 16 plastic pots were randomly divided into four groups of control group, test group 1, test group 2 and test group 3, with 4 plastic pots in each group, and the following experiment was performed:
control (i.e., CK): 3 corn buds prepared in the first step are sown in each plastic basin, and 50mL of DSE inactivated bacteria liquid and 50g of glomus versicolor inactivated bacteria agent are added into each plastic basin;
trial group 1 (i.e. DSE 20% + AM or DSE 20% + AMF): sowing 3 corn sprouts prepared in the first step in each plastic pot, and simultaneously adding a microbial agent A into each plastic pot;
trial group 2 (i.e. DSE 40% + AM or DSE 40% + AMF): 3 corn sprouts prepared in the first step are sown in each plastic basin, and meanwhile, a microbial agent B is added into each plastic basin;
trial group 3 (i.e. DSE 80% + AM or DSE 80% + AMF): and (3) sowing the corn sprouts prepared in the first step in each plastic pot, and simultaneously adding a microbial inoculum C into each plastic pot.
5. After step 4, the soil was watered quantitatively and the soil moisture was about 70% of the maximum water holding capacity of the soil. After emergence, the number of seedlings in each pot is 1. Respectively collecting corn thermal infrared images (the collection time is 12: 00) by a thermal infrared imager at 35d, 44d and 57d after the corn seedlings emerge, and then processing the corn thermal infrared images by FLIR research IR Max software (the thermal infrared imager is carried by the thermal infrared imager) to obtain the canopy temperature of each corn plant; and finally, calculating the average value according to the groups to obtain the canopy temperature of each group of corns. In addition, the plant height was measured after harvesting corn, then the roots, stems, leaves were separated, and the dry weight was measured separately (dried at 80 ℃ for 48 hours), and the average value was calculated by group.
The corn thermal infrared image at 35d after corn emergence is shown in figure 1.
The selected region of interest when the thermal infrared image of the corn at 35d after the corn seedling emergence is processed by software is shown in figure 2.
The canopy temperature obtained when the thermal infrared image of the corn at 35d after emergence of the corn was processed with the software is shown in fig. 3.
The statistics of the canopy temperature at 35d, 44d and 57d after emergence of each group of corn are shown in table 2 and fig. 4.
TABLE 2
Note: the lower case letters in the same column indicate significant difference (P < 0.05).
The results of the determination of the dry root weight, stem weight, dry leaf weight and plant height of each group of maize plants are shown in Table 3.
TABLE 3
| Root weight (g) | Stem Dry weight (g) | Leaf Dry weight (g) | Plant height (cm) | |
| Control group | 0.74±0.29b | 0.24±0.15b | 1.08±0.24b | 35.47±4.83b |
| Test group 1 | 3.38±0.63b | 0.58±0.08a | 3.52±0.89b | 61.33±5.69a |
| |
3.65±0.50b | 0.65±0.09a | 3.16±0.46b | 62.4±1.92a |
| |
4.15±0.79a | 1.92±0.84a | 4.20±0.75a | 65.63±5.27a |
Note: the lower case letters in the same column indicate significant difference (P < 0.05).
The correlation results of the canopy temperature at 57d after emergence of each group of corn with the total biomass (total biomass ═ dry weight of roots + dry weight of stems + dry weight of leaves) are shown in fig. 5.
The correlation results of the canopy temperature and the plant height at 57d after emergence of each group of corn are shown in FIG. 6.
The experimental results are specifically as follows:
(1) the 35 th and 57 th days after the corn seedlings emerge, and the canopy temperature of the control group and the test group 1 is basically the same; at 44d after the emergence of the corn seedlings, the canopy temperature of the test group 1 is obviously lower than that of the control group; 35d, 44d and 57d after corn seedling emergence, the canopy temperature of the test group 2 and the test group 3 is obviously lower than that of the control group, and the canopy temperature of the test group 3 is lower than that of the test group 2.
(2) There is a negative correlation between canopy temperature and total biomass. The total biomass, root dry weight and leaf dry weight of test group 3 were higher than those of test group 2.
(3) The temperature of the canopy layer and the plant height have a negative correlation. The plant heights of the test group 1, the test group 2 and the test group 3 are all obviously higher than those of the control group, and the plant heights are increased to a certain extent along with the increase of the DSE culture solution.
The above results indicate that there is no significant difference in plant height among test group 1, test group 2 and test group 3, but the substance accumulation ability of test group 3 is the strongest (i.e., the greatest total biomass), and thus the dose of the microbial agent c administered in test group 3 is considered to be the best. As the plant height and the total biomass belong to indexes obtained in the mature period of the corn, in order to save manpower, material resources and financial resources, the thermal infrared imager can be used for detecting the temperature of the canopy after 40 days of corn seedling emergence (such as 44 days after the corn seedling emergence and 57 days after the corn seedling emergence), so that the effect of the microbial agent on promoting the plant growth is evaluated, namely the optimal application dose of the microbial agent is obtained. The lower the temperature of the canopy layer after 40 days after the corn seedling emergence is, the better the effect of the microbial agent on promoting the plant growth is, and the optimal application dose of the microbial agent is.
<110> Beijing Synbiotic ecological engineering technology Co., Ltd, China university of mining industry (Beijing)
<120> method for acquiring applied dose of microorganism AM and DSE fungus combined microbial inoculum by thermal infrared monitoring
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 608
<212> DNA
<213> Alternaria alternata (Alternaria sp.)
<400> 1
cccttccgta gggtgaacct gcggagggat cattacacaa tatgaaagcg ggctggatac 60
tctgtagtag tggattgctt tacggcgtgc gctgctggag agcctagcct tgctgaatta 120
ttcacccgtg tcttttgcgt acttcttgtt tccttggtgg gctcgcccgc cacaaggaca 180
actcataaac cttttgtaat agcaatcagc gtcagtaaca acataataat tacaactttc 240
aacaacggat ctcttggttc tggcatcgat gaagaacgca gcgaaatgcg atacgtagtg 300
tgaattgcag aattcagtga atcatcgaat ctttgaacgc acattgcgcc ctttggtatt 360
ccaaagggca tgcctgttcg agcgtcattt gtaccctcaa gctttgcttg gtgttgggcg 420
tcttgtctcc agtccgctgg agactcgcct taaagtcatt ggcagccggc ctactggttt 480
cggagcgcag cacaagtcgc actcttttcc agccaaggtc agcgtccaac aagccttttt 540
tcaacttttg acctcggatc aggtagggat acccgctgaa cttaagcata tcaataagcg 600
gaggaaaa 608
Claims (5)
1. A method of obtaining an applied dose of a microbial agent, comprising the steps of:
(1) sowing a plurality of plant seeds and applying microbial agents with different dosages;
(2) after the step (1) is finished, culturing, and determining the application dose of the microbial agent according to the temperature of the canopy of the plant;
the 'canopy temperature of the plant' is the canopy temperature after 40 days after the plant seedlings emerge;
the judgment principle of determining the applied dose of the microbial agent according to the temperature of the canopy of the plant is that the lower the temperature of the canopy of the plant is, the better the effect of the microbial agent on promoting the growth of the plant is;
the plant is corn;
the microbial agents include Alternaria sp (Alternaria sp.) fungus and Glomus versiforme (Glomus versiforme) BGC NM 04B;
the Alternaria (Alternaria sp.) fungus is Alternaria (Alternaria sp.)001, and the preservation number of the fungus in the common microorganism center of the China general microbiological culture Collection center is CGMCC No. 17463.
2. A method for preparing a microbial agent with a plant growth promoting function comprises the following steps:
(a1) mixing the components according to different proportions to obtain corresponding microbial agents;
(a2) treating the plants with the microbial agents prepared in step (a1), respectively;
(a3) after the step (a2) is completed, culturing, and determining the composition of the microbial agent according to the temperature of the canopy of the plant;
the 'canopy temperature of the plant' is the canopy temperature after 40 days after the plant seedlings emerge;
the judgment principle of determining the composition of the microbial agent according to the canopy temperature of the plant is that the lower the canopy temperature of the plant is, the better the effect of the microbial agent on promoting the growth of the plant is;
the microbial agents include Alternaria (Alternaria sp) fungus and glomus terrestris BGC NM 04B;
the Alternaria (Alternaria sp.) fungus is Alternaria (Alternaria sp.)001, and the preservation number of the fungus in the China general microbiological culture Collection center is CGMCC No. 17463; the plant is corn.
3. The method of claim 1 or 2, wherein: the corn is a glutinous rice No. one of corn varieties.
4. The method of claim 1 or 2, wherein: when the plant is waxy I in a maize variety, the optimal dose of microbial agent applied per maize is 1300 spores of Gliocladium geotrichum and 124.4mg dry matter of Alternaria sp.
5. Use of the method of any one of claims 1-4, being at least one of b1) to b 3):
b1) plant production;
b2) planting plants;
b3) regulating the growth of the plant;
the plant is corn.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910505650.4A CN110260976B (en) | 2019-06-12 | 2019-06-12 | Method for obtaining applied dose of microorganism AM and DSE fungus combined microbial inoculum by thermal infrared monitoring |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910505650.4A CN110260976B (en) | 2019-06-12 | 2019-06-12 | Method for obtaining applied dose of microorganism AM and DSE fungus combined microbial inoculum by thermal infrared monitoring |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110260976A CN110260976A (en) | 2019-09-20 |
| CN110260976B true CN110260976B (en) | 2021-08-31 |
Family
ID=67917881
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910505650.4A Active CN110260976B (en) | 2019-06-12 | 2019-06-12 | Method for obtaining applied dose of microorganism AM and DSE fungus combined microbial inoculum by thermal infrared monitoring |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110260976B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110205248B (en) * | 2019-06-12 | 2021-04-27 | 中国矿业大学(北京) | Method for promoting plant growth by jointly inoculating AM and DSE fungi and microbial agent used by method |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6212824B1 (en) * | 1991-10-18 | 2001-04-10 | Dekalb Genetics Corporation | Methods for classifying plants for evaluation and breeding programs by use of remote sensing and image analysis technology |
| CN101984067A (en) * | 2010-10-19 | 2011-03-09 | 中国农业大学 | Method of detecting growth promoting effect of plant rhizosphere growth promoting bacteria |
| CN107494259A (en) * | 2017-10-11 | 2017-12-22 | 石家庄市农林科学研究院 | A kind of field saving irrigation model wheat breed evaluation and screening method |
| CN107734970A (en) * | 2015-07-03 | 2018-02-23 | 洛桑研究所有限公司 | Handle the water stress in plant |
| CN110205249A (en) * | 2019-06-12 | 2019-09-06 | 中国矿业大学(北京) | A kind of method promoting plant growth and its rod method fungi used |
| CN110205248A (en) * | 2019-06-12 | 2019-09-06 | 中国矿业大学(北京) | A kind of method of simultaneous inoculation AM and the promotion plant growth of DSE fungi and its microbial bacterial agent used |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101539531B (en) * | 2009-04-09 | 2011-11-16 | 浙江大学 | Rice leaf blast detection and classification method based on multi-spectral image processing |
| CN201503402U (en) * | 2009-09-30 | 2010-06-09 | 浙江大学 | Thermal infrared image diagnosis system for sclerotinia rot of colza |
| CA3195750A1 (en) * | 2013-11-06 | 2015-05-14 | The Texas A & M University System | Fungal endophytes for improved crop yields and protection from pests |
| CN110243478B (en) * | 2019-06-12 | 2021-01-15 | 中国矿业大学(北京) | Method for acquiring DSE applied dose by thermal infrared monitoring |
-
2019
- 2019-06-12 CN CN201910505650.4A patent/CN110260976B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6212824B1 (en) * | 1991-10-18 | 2001-04-10 | Dekalb Genetics Corporation | Methods for classifying plants for evaluation and breeding programs by use of remote sensing and image analysis technology |
| CN101984067A (en) * | 2010-10-19 | 2011-03-09 | 中国农业大学 | Method of detecting growth promoting effect of plant rhizosphere growth promoting bacteria |
| CN107734970A (en) * | 2015-07-03 | 2018-02-23 | 洛桑研究所有限公司 | Handle the water stress in plant |
| CN107494259A (en) * | 2017-10-11 | 2017-12-22 | 石家庄市农林科学研究院 | A kind of field saving irrigation model wheat breed evaluation and screening method |
| CN110205249A (en) * | 2019-06-12 | 2019-09-06 | 中国矿业大学(北京) | A kind of method promoting plant growth and its rod method fungi used |
| CN110205248A (en) * | 2019-06-12 | 2019-09-06 | 中国矿业大学(北京) | A kind of method of simultaneous inoculation AM and the promotion plant growth of DSE fungi and its microbial bacterial agent used |
Non-Patent Citations (3)
| Title |
|---|
| Comparison of canopy temperature-based water stress indices for maize;Kendall C. Dejonge等;《Agricultural Water Management》;20150601;第156卷(第1期);51-62 * |
| 不同施肥措施对夏玉米田间群体微环境的影响;聂胜委等;《山西农业科学》;20170120;第45卷(第1期);54-59 * |
| 花生、玉米不同间作方式对连作花生生理特性及产量品质的影响;姚远;《中国优秀硕士学位论文全文数据库》;20180115;D047-397 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110260976A (en) | 2019-09-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101822272B (en) | Streptomyces griseoflavus and application thereof in biological prevention and control of plant diseases | |
| CN110205248B (en) | Method for promoting plant growth by jointly inoculating AM and DSE fungi and microbial agent used by method | |
| CN106497831B (en) | A kind of prevention and treatment Phytophthora nicotianae disease composite bacteria agent and its preparation method and application | |
| CN111763629B (en) | Bacillus belgii and application thereof | |
| CN105543132A (en) | Bacillus methylotrophicus YB-F7 and application thereof in preventing plant diseases | |
| CN105368720B (en) | Cotton endogenetic fungus CEF-082 and its application in cotton verticillium wilt prevention and treatment | |
| CN106190917A (en) | One strain broad-spectrum disease resistance growth-promoting degeneration-resistant product nitrogen pseudomonas and application thereof | |
| US20220369648A1 (en) | Endophytic falciphora oryzae fo-r20 and its application | |
| CN106520908A (en) | Identification method for clubroot resistance of alpine radish at seedling stage | |
| CN105154339A (en) | Trichoderma viride strain and application thereof | |
| CN105132296A (en) | Hook-like trichoderma strain and application thereof | |
| CN111778174B (en) | Bacillus subtilis with inhibiting effect on citrus sand skin disease and screening method thereof | |
| CN110205249B (en) | Method for promoting plant growth and alternaria alternate fungus used by same | |
| CN110260976B (en) | Method for obtaining applied dose of microorganism AM and DSE fungus combined microbial inoculum by thermal infrared monitoring | |
| Nguyen et al. | Does inoculation with native rhizobia enhance nitrogen fixation and yield of cowpea through legume-based intercropping in the northern mountainous areas of Vietnam? | |
| CN110243478B (en) | Method for acquiring DSE applied dose by thermal infrared monitoring | |
| Kempenaar | Studies on biological control of Chenopodium album by Ascochyta caulina | |
| CN105439657A (en) | Preparation method for strawberry dedicated anti-continuous cropping biological organic fertilizer | |
| CN105316258A (en) | Strain 1LN2 for preventing and treating rice sheath blight disease and application of strain 1LN2 | |
| CN105002111B (en) | It is a kind of prevent and treat cucumber downy mildew bacterial strain TDJN1 and its application | |
| CN109055236B (en) | Isaria pinicola WSWM1171 and application thereof in prevention and control of potato corm moth pupae | |
| CN102329758B (en) | Biological prevention bacterial strain BS1 (bacillus cereus) for preventing and controlling greenhouse cucumber downy mildew and application thereof | |
| CN110257254A (en) | A kind of preparation method and application of biocontrol bacterial strain TMN-1 and biocontrol agent | |
| CN110205262A (en) | Hydrogenlike silicon ion A2 bacterial strain, biocontrol agent and its preparation method and application | |
| Yan et al. | Simulation of the spreading trend of Camellia oleifera Anthracnose in Guangdong Province |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |