CN109997450A - A kind of production method promoting cigarette quality using addition probiotics - Google Patents
A kind of production method promoting cigarette quality using addition probiotics Download PDFInfo
- Publication number
- CN109997450A CN109997450A CN201910269999.2A CN201910269999A CN109997450A CN 109997450 A CN109997450 A CN 109997450A CN 201910269999 A CN201910269999 A CN 201910269999A CN 109997450 A CN109997450 A CN 109997450A
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- China
- Prior art keywords
- tobacco
- production method
- cigarette quality
- probiotics
- seed
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/45—Tobacco
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Soil Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of production method for promoting cigarette quality using addition probiotics, bacillus preparation preparations；Tobacco seed seed dressing；The transplanting of tobacco root dipping.The beneficial effects of the invention are as follows utilization tobacco seeds and bacillus subtilis to dress seed, while in transplanting time root dipping, obtaining the rhizosphere soil tobacco seedlings for carrying bacillus subtilis.The healthy growth after tobacco transplant to crop field, is not easy susceptible, and wildfire and rust to tobacco have a good resistance, and it is simple that the present invention is inoculated with probiotics technical operation, is easy to implement.For probiotics used convenient for culture, anti-adversity is strong.
Description
Technical field
The invention belongs to tobacco planting technical fields, are related to a kind of production side that cigarette quality is promoted using addition probiotics
Method.
Background technique
Tobacco is a kind of important industrial crops, and along with the generation of pest and disease damage during tobacco planting, this is certain
Reduce the yield of tobacco in degree, simultaneously because the application of pesticide, forms influence to cigarette quality, also increase tobacco grower's
Workload and planting cost.As the improvement of people's living standards, the specific gravity of tobacco in people's lives is increasing.Many institute's weeks
Know, containing there are many substance being harmful to the human body inside tobacco, mainly there is nicotine, smoke tar, carbon monoxide etc., in fact, people
Usually ignore there are also pesticide residue inside tobacco, these pesticide residues also can generate potential hazard to human body.Pesticide residue
It contacts further closely, shows in carcinogenic factor according to medical research, environmental factor accounts for about 80%, in environment with the health of the mankind
In factor, toxic chemical substance accounts for about 95% or more, it can be seen that, Pesticides Residues are very seriously to the life of the mankind
It deposits and constitutes threat with health.Pesticide Residue is quite serious in current China tobacco leaf production, and Hunan Province's pesticide is residual within 2011
It stays in examining report and points out, it is up to as many as 37 kinds of the type of pesticide residue, exceeded in 58 parts of inspection tobacco samples of its detection
Rate 23.3%, the pesticide residue that tobacco detects are mainly organic phosphorus and organochlorine residue, these pesticides are severe toxicity, high poison,
And residual device is relatively long.
Summary of the invention
It is of the invention the purpose of the present invention is to provide a kind of production method for promoting cigarette quality using addition probiotics
Beneficial effect is to be dressed seed using tobacco seed and bacillus subtilis, while in transplanting time root dipping, obtaining and carrying bacillus subtilis
The rhizosphere soil tobacco seedlings of bacterium.The healthy growth after tobacco transplant to crop field, is not easy susceptible, has to the wildfire and rust of tobacco
Good resistance, present invention inoculation probiotics technical operation is simple, is easy to implement.Probiotics used is convenient for culture, anti-adversity
By force.
The technical scheme adopted by the invention is that following the steps below:
(1) prepared by bacillus preparation；
(2) tobacco seed is dressed seed；
(3) tobacco root dipping is transplanted.
Further, bacterial strain used in step (1) be Bacillussubtilis (Chinese agriculture Organism Depositary,
Strain number ACCC01185), bacteria suspension concentration is 1 × 108CFU/ml。
Further, 100g tobacco seed/10ml bacillus preparation in step (2).
Further, will prepare the tobacco seedlings migrated in step (3) in nursery in concentration is 1 × 108CFU/ml bacillus bacterium
Root dipping in agent.
Detailed description of the invention
Fig. 1 is the hydrogen peroxide enzyme activity of the superoxide dismutase of tobacco and the tobacco seedlings containing profitable probliotics under nature cropping pattern
Property contrast schematic diagram；
Fig. 2 is the chlorophyll concentration of mature later period tobacco chlorophyll concentration and the tobacco seedlings containing profitable probliotics under nature cropping pattern
Contrast schematic diagram；
Fig. 3 is the disease incidence situation schematic diagram of tobacco；
Fig. 4 is the dry matter weight schematic diagram of tobacco；
Fig. 5 is the content schematic diagram of the important fragrance matter alkaloid of tobacco.
Specific embodiment
The present invention is described in detail With reference to embodiment.
Embodiment 1
The present invention using addition probiotics promoted cigarette quality production method the following steps are included:
1, prepared by bacillus preparation
By Chinese agriculture Organism Depositary, strain number is that ACCC01185 bacterial strain carries out inoculation activation, and culture medium is
Beef extract-peptone bacteria culture media is seeded in shaking flask after activating 3 times and carries out fermented and cultured, passes through Glanz dyeing and blood cell
Tally counts bacillus, calculates colony density, and colony density reaches 1 × 10 after about culture 10h8CFU/ml。
2, tobacco seed and bacillus subtilis formulation seed dressing, sowing
After tobacco seed 1000g is cleaned with distilled water and the gemma microbial inoculum of 100ml carries out mixing seed dressing, is sufficiently stirred
Uniformly, tobacco seed is kept to show to moisten.
20 degree of environment temperature or so, after field plot is put in order, decomposed biological organic fertilizer is added as base fertilizer, according to conjunction
Reason density is sowed, and is sprinkled profoundly water after sowing, covering mulch heat preservation.
3, tobacco seedlings root is dipped into bacillus subtilis formulation before transplanting
After tobacco seedlings grow to suitable transplanting crop field and 25 degree of daily mean temperature or more, the liquid of 5L is prepared according to step 1 fermentation
Bacillus preparation, fermentation to 1 × 108CFU/ml is upside down in plastic barrel later, and when transplanting dips in tobacco seedlings root in bucket
It takes, is directly transplanted to crop field, watering soil moisture conservation later.
4, the tobacco seedlings under tobacco seedlings and natural cropping pattern of the rhizosphere for obtaining the present invention containing profitable probliotics compare, real
It is as follows to test result schematic diagram:
Fig. 1 shows the hydrogen peroxide of the superoxide dismutase of tobacco and the tobacco seedlings containing profitable probliotics under nature cropping pattern
Enzymatic activity comparison, the tobacco resistance containing profitable probliotics is strong, and hydrogen peroxide enzyme activity is high.
Fig. 2 shows the chlorophyll of mature later period tobacco chlorophyll concentration and the tobacco seedlings containing profitable probliotics under nature cropping pattern
Concentrations versus, the Tobacco resistance containing profitable probliotics is strong, and leaf color is dark green compared with Nicotiana tabacum.
Fig. 3 shows the disease incidence situation of tobacco, (is not added with the prebiotic of cultivated spore preparation in field random inspection control group
Bacterium) and 500 plants of tobaccos of experimental group (addition bacillus), the disease incidence of its rust and wildfire is calculated separately in the maturity period,
By comparing discovery, the disease incidence of control group is 19.5%, and the disease incidence of experimental group is 5% or so.Experimental group obtains preferable
Anti- rust and wildfire effect.
Fig. 4 shows the dry matter weight of tobacco, in control group (probiotics for being not added with cultivated spore preparation) and experimental group (addition
Bacillus) tobacco leaf of growth conditions always 40 or so is taken respectively, it is crushed under constant humidity, every group of tobacco after equivalent
Weight in wet base is 2000.00g.Dry matter weight is measured after being respectively dried, the tobacco leaf of the addition probiotics of survey is obviously than control group leaf
Piece dry matter weight.
Fig. 5 shows the content of the important fragrance matter alkaloid of tobacco, analyzes a certain number of control groups respectively and (does not add
Add the probiotics of cultivated spore preparation) and the tobacco in experimental group (addition bacillus) crop field in alkaloid, by score
Analysis is found, the content of alkaloid in tobacco can be significantly improved after addition probiotics.
The above is only not to make limit in any form to the present invention to better embodiment of the invention
System, any simple modification that embodiment of above is made according to the technical essence of the invention, equivalent variations and modification,
Belong in the range of technical solution of the present invention.
Claims (4)
1. a kind of production method for promoting cigarette quality using addition probiotics, it is characterised in that the following steps are included:
(1) prepared by bacillus preparation；
(2) tobacco seed is dressed seed；
(3) tobacco root dipping is transplanted.
2. a kind of production method for promoting cigarette quality using addition probiotics according to claim 1, it is characterised in that: institute
Stating bacterial strain used in step (1) is Bacillus subtilis (Chinese agriculture Organism Depositary, strain number ACCC
01185), bacteria suspension concentration is 1 × 108CFU/ml。
3. a kind of production method for promoting cigarette quality using addition probiotics according to claim 1, it is characterised in that: institute
State 100g tobacco seed/10ml bacillus preparation in step (2).
4. a kind of production method for promoting cigarette quality using addition probiotics according to claim 1, it is characterised in that: institute
The tobacco seedlings migrated will be prepared in concentration by stating in nursery in step (3) be 1 × 108Root dipping in CFU/ml gemma bacillus agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910269999.2A CN109997450A (en) | 2019-04-04 | 2019-04-04 | A kind of production method promoting cigarette quality using addition probiotics |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910269999.2A CN109997450A (en) | 2019-04-04 | 2019-04-04 | A kind of production method promoting cigarette quality using addition probiotics |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109997450A true CN109997450A (en) | 2019-07-12 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910269999.2A Pending CN109997450A (en) | 2019-04-04 | 2019-04-04 | A kind of production method promoting cigarette quality using addition probiotics |
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| Country | Link |
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| CN (1) | CN109997450A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115322930A (en) * | 2022-08-19 | 2022-11-11 | 遵义大兴复肥有限责任公司 | Soil probiotics for promoting tobacco seedling growth |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1554744A (en) * | 2003-12-28 | 2004-12-15 | 何月秋 | Bacillus subtilis strain |
| CN102154186A (en) * | 2011-04-14 | 2011-08-17 | 中国农业科学院烟草研究所 | Bacillus subtilis and use thereof in prevention and control of fungus disease |
| CN102388921A (en) * | 2011-08-15 | 2012-03-28 | 中国农业科学院烟草研究所 | Bacillus subtilis Tpb55 wettable powder and application |
| CN104745517A (en) * | 2015-04-10 | 2015-07-01 | 安徽农业大学 | Special potassium-dissolving bacterium for tobaccos and fungicide of special potassium-dissolving bacterium |
| KR20150136273A (en) * | 2014-05-27 | 2015-12-07 | 대한민국(농촌진흥청장) | Constitutive promoter Br16 |
-
2019
- 2019-04-04 CN CN201910269999.2A patent/CN109997450A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1554744A (en) * | 2003-12-28 | 2004-12-15 | 何月秋 | Bacillus subtilis strain |
| CN102154186A (en) * | 2011-04-14 | 2011-08-17 | 中国农业科学院烟草研究所 | Bacillus subtilis and use thereof in prevention and control of fungus disease |
| CN102388921A (en) * | 2011-08-15 | 2012-03-28 | 中国农业科学院烟草研究所 | Bacillus subtilis Tpb55 wettable powder and application |
| KR20150136273A (en) * | 2014-05-27 | 2015-12-07 | 대한민국(농촌진흥청장) | Constitutive promoter Br16 |
| CN104745517A (en) * | 2015-04-10 | 2015-07-01 | 安徽农业大学 | Special potassium-dissolving bacterium for tobaccos and fungicide of special potassium-dissolving bacterium |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115322930A (en) * | 2022-08-19 | 2022-11-11 | 遵义大兴复肥有限责任公司 | Soil probiotics for promoting tobacco seedling growth |
| CN115322930B (en) * | 2022-08-19 | 2023-06-09 | 遵义大兴复肥有限责任公司 | Soil probiotics for promoting tobacco seedling growth |
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Application publication date: 20190712 |