CN115322930A - Soil probiotics for promoting tobacco seedling growth - Google Patents
Soil probiotics for promoting tobacco seedling growth Download PDFInfo
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- CN115322930A CN115322930A CN202210999079.8A CN202210999079A CN115322930A CN 115322930 A CN115322930 A CN 115322930A CN 202210999079 A CN202210999079 A CN 202210999079A CN 115322930 A CN115322930 A CN 115322930A
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The invention discloses soil probiotics for promoting tobacco seedling growth, belonging to the technical field of microorganisms, and comprising the following strains:Bacillus zanthoxyli ZY2, preservation unit: china center for type culture Collection, preservation date: 2021, 7/19, accession No.: CCTCC NO: M2021897. Through tests and determination, the soil probiotics for promoting the growth of tobacco seedlings can promote the growth of tobacco seedlings in the small cross stage and the large cross stage of flue-cured tobacco respectively, and particularly the growth of roots and the improvement of the activity of the roots are obviously improved. In addition, after the microbial inoculum is applied, the catalase activity and the soluble protein content are obviously increased, which indicates that the stress resistance of the tobacco seedlings is enhanced.
Description
Technical Field
The invention relates to the technical field of microorganisms, in particular to soil probiotics for promoting tobacco seedling growth.
Background
Tobacco is a plant of the genus nicotiana of the family solanaceae, usually an annual herb, and the main value of tobacco is to produce flue-cured tobacco from its leaves and stems and to process it into cigarettes. Tobacco is cultivated in various provinces of China, and is an important economic crop.
The root system is an important guarantee for the health and high yield of the overground part of the plant (tobacco). Nutrient, moisture and other substances necessary for plant growth are mainly absorbed through root systems, so people are always looking for methods for improving the nutrient and moisture absorption of root systems, and these methods mainly include expanding the area of root systems, enhancing the activity of root systems and the like, such as loosening soil and applying fertilizer to create a good soil environment for root system growth, adding plant growth regulators to improve the activity of root systems, and recently, research focuses on soil probiotics.
The soil probiotics have the main functions of promoting the release of elements such as nitrogen, phosphorus and potassium, stimulating the growth of crops, killing insects and sterilizing, increasing the resistance of crops and the like, and the types which are common at present comprise bacillus subtilis, bacillus megaterium, bacillus thuringiensis, bacillus licheniformis, bacillus laterosporus, bacillus mucilaginosus, streptomyces jingyangensis, azotobacter vinelandii, mycorrhizal fungi, lactobacillus and the like. The research on the screening, function definition and applicable environment of soil beneficial bacteria is always a research hotspot of agricultural production since the last 80 centuries.
Microorganisms have certain selectivity on ecological environment, and although a large amount of soil-beneficial microbial agents or fertilizers appear in the market, the effect can be good only in a limited area, so that the wide popularization of beneficial microorganisms is difficult.
Disclosure of Invention
The invention aims to provide soil probiotics for promoting tobacco seedling growth, and the strain name is as follows:Bacillus zanthoxyliZY2, preservation unit: china center for type culture Collection, preservation date: 2021, 7/19, accession No.: CCTCC NO: M2021897.
Through tests and determination, the soil probiotics for promoting the growth of tobacco seedlings can promote the growth of tobacco seedlings in the small cross stage and the large cross stage of flue-cured tobacco respectively, and particularly the growth of root systems and the improvement of the activity of the root systems are obvious. In addition, after the microbial inoculum is applied, the catalase activity and the soluble protein content are obviously increased, which indicates that the stress resistance of the tobacco seedlings is enhanced.
Drawings
FIG. 1 is a photograph showing the growth of tobacco seedlings in 7 days after the soil probiotics for promoting the growth of tobacco seedlings according to the invention are applied to the tobacco cross-stage microbial inoculum in the example.
FIG. 2 is a photograph showing the root growth status of tobacco seedlings after the soil probiotics for promoting the growth of tobacco seedlings of the present invention are applied to the tobacco megacross inoculant for 7 days in the examples.
FIG. 3 is a comparison result of root activity of cured tobacco seedlings which are applied with the soil probiotics for promoting tobacco seedling growth for 7d in the embodiment of the invention to the tobacco cross stage microbial inoculum.
FIG. 4 is the comparison result of catalase activity of tobacco seedlings applied 7d to tobacco crisscross inoculants in the examples by the soil probiotics for promoting tobacco seedling growth of the invention.
FIG. 5 is a comparison result of soluble protein content of tobacco seedlings which are applied to the tobacco cross stage microbial inoculum for 7 days in the embodiment of soil probiotics for promoting tobacco seedling growth.
FIG. 6 is a photograph showing growth conditions of tobacco seedlings in seedling stage after 7 days of microbial inoculum application to tobacco cross stage microbial inoculum in the example of soil probiotics for promoting tobacco seedling growth of the invention.
Detailed Description
The technical solution of the present invention will be clearly and completely described below with reference to the specific embodiments of the present invention. The described embodiments are only some embodiments of the invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention relates to a soil probiotics for promoting tobacco seedling growth, which comprises the following strains:Bacillus zanthoxyliZY2, preservation unit: china center for type culture Collection, preservation date: 2021, 7/19, accession No.: CCTCC NO: M2021897.
Design of experiments
Adopting single-factor test, respectively applying ZY2 microbial inoculum treatment to tobacco seedlings in small cross stage and large cross stage, and repeating for 3 times without applying microbial inoculum as Control (CK). Wherein the viable count of ZY2 microbial inoculum is more than or equal to 100 hundred million g -1 。
The seedling raising trays in the floating seedling raising pond are separated by white plastic cloth and divided into 6 cells, and 3 160-hole expanded polystyrene floating trays with the specification of 57 cm multiplied by 36 cm multiplied by 6 cm are placed in each cell.
Diluting ZY2 microbial inoculum to 10 of original bacterial fluid concentration -1 Adding the bacterial agent (the application amount of each tobacco seedling is about 5 ml) into the seedling substrate. And observing the growth and development conditions of the tobacco seedlings every day after the microbial inoculum is applied, and recording the growth potential of the tobacco seedlings. Selecting 15 representative tobacco seedlings in each cell, recording the fresh weight, dry weight, root crown ratio, plant height, stem circumference, maximum leaf length, maximum leaf width, maximum leaf area, leaf number, chlorophyll content of the first leaf/the second leaf, the maximum root length, root stem/fresh weight, root surface area, root volume and root activity of main root indexes of tobacco seedlings treated differently, and measuring the physiological indexes of the root system of the tobacco seedlings mainly comprises Catalase (CAT) activity and soluble protein content (SP).
Measurement method
2.1 determination of agronomic traits and root system indexes of tobacco seedlings
And (5) photographing, recording, sampling and measuring after the microbial inoculum is applied for 7 d. Wherein, the agronomic characters of the tobacco seedlings are determined according to Chinese tobacco industry standard YC/T142-2010; the root surface area and root volume were determined using a root system scanner (model: epson expression 1000 xl); measuring chlorophyll by using a chlorophyll meter; root system activity was measured by triphenyltetrazolium chloride reduction method (TTC method).
Determination of indicators associated with resistance
And selecting 15 representative tobacco seedlings in each cell, and applying the microbial inoculum for 7 days to measure the tobacco seedlings CATA and SP.
Measurement results
A strain of Bacillus zanthoxyli (Bacillus zanthoxyli) is screened from tobacco-planting soil in Zunyi in Guizhou, and the strain can promote the growth of tobacco seedlings in the small cross stage and the large cross stage of flue-cured tobacco (shown in figure 1, table 1 and figure 6), particularly the root growth (shown in table 1 and figure 2) and the root activity (shown in figure 3) are obviously improved. In addition, after the microbial inoculum is applied, the catalase activity (figure 4) and the soluble protein content (figure 5) are obviously increased, which indicates that the stress resistance of the tobacco seedlings is enhanced.
FIG. 1 is a photograph showing the growth of tobacco seedlings in 7 days after the soil probiotics for promoting the growth of tobacco seedlings according to the present invention are applied to the tobacco cross-bud microbial inoculum in the examples.
FIG. 2 is a photograph showing the root growth status of tobacco seedlings after the soil probiotics for promoting the growth of tobacco seedlings according to the present invention are applied to the tobacco grown cross inoculant for 7 days in the example.
FIG. 3 shows the comparison result of the root activity of cured tobacco seedlings which are applied 7 days after the soil probiotics for promoting the growth of tobacco seedlings are applied to the tobacco cross-stage inoculant in the embodiment.
FIG. 4 shows the comparison result of catalase activity of tobacco seedlings applied 7 days after the soil probiotics for promoting tobacco seedling growth of the invention to the tobacco macroprozoic agent in the examples.
FIG. 5 shows the results of comparing the soluble protein content of tobacco seedlings applied 7 days after the soil probiotics for promoting the growth of tobacco seedlings according to the present invention are applied to the tobacco macroprojectile agent in the examples.
FIG. 6 is a photograph showing growth conditions of tobacco seedlings in seedling stage after 7 days of microbial inoculum application to tobacco cross-shaped microbial inoculum in the example of soil probiotics for promoting tobacco seedling growth of the present invention.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present specification describes embodiments, not every embodiment includes only a single embodiment, and such description is for clarity purposes only, and it is to be understood that all embodiments may be combined as appropriate by one of ordinary skill in the art to form other embodiments as will be apparent to those of skill in the art from the description herein.
Claims (1)
1. A soil probiotics for promoting tobacco seedling growth, the strain name is:Bacillus zanthoxyliZY2, depository: china center for type culture Collection, preservation date: 2021, 7/19, accession No.: CCTCC NO: M2021897.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103087944A (en) * | 2012-12-07 | 2013-05-08 | 贵州省烟草公司毕节市公司 | Bacilluscereus and application thereof |
| CN109997450A (en) * | 2019-04-04 | 2019-07-12 | 青岛农业大学 | A kind of production method promoting cigarette quality using addition probiotics |
| CN113201474A (en) * | 2021-04-26 | 2021-08-03 | 中国农业科学院烟草研究所 | Bacillus subtilis TBWR1, application thereof and obtained control agent |
| KR20220067077A (en) * | 2020-11-17 | 2022-05-24 | 동아대학교 산학협력단 | Bacillus zanthoxyli strain promoting tolerance of plants and use thereof |
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- 2022-08-19 CN CN202210999079.8A patent/CN115322930B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103087944A (en) * | 2012-12-07 | 2013-05-08 | 贵州省烟草公司毕节市公司 | Bacilluscereus and application thereof |
| CN109997450A (en) * | 2019-04-04 | 2019-07-12 | 青岛农业大学 | A kind of production method promoting cigarette quality using addition probiotics |
| KR20220067077A (en) * | 2020-11-17 | 2022-05-24 | 동아대학교 산학협력단 | Bacillus zanthoxyli strain promoting tolerance of plants and use thereof |
| CN113201474A (en) * | 2021-04-26 | 2021-08-03 | 中国农业科学院烟草研究所 | Bacillus subtilis TBWR1, application thereof and obtained control agent |
Non-Patent Citations (3)
| Title |
|---|
| ALISHER USMONOV ET AL.: ""The Bacillus zc}nthoxyli HS1 Strain Renders Vegetable Plants Resistant and Tolerant against Pathogen Infection and High Salinity Stress"", 《THE PLANT PATHOLOGY JOURNAL》 * |
| 易有金等: ""内生枯草芽孢杆菌B-001对烟草幼苗的 促生作用及其生长动态"", 《植物保护学报》 * |
| 肖雨沁等: ""三种芽孢杆菌菌剂对烤烟育苗效果的影响"", 《中国农业科技导报》 * |
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