CN105255769A - Enterobacter cloacae and application thereof - Google Patents
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Abstract
The invention discloses enterobacter cloacae and an application thereof. The strain is classified and named as enterobacter cloacae JP6 and is collected in China General Microbiological Culture Collection Center (CGMCC) in No.3, No.1 Courtyard, Beichen West Road, Chaoyang District, Beijing, with collection date of August 28, 2014 and collection number of CGMCC No.9615. The strain has good phosphate solubilizing effects. Ultraviolet spectrophotometry shows that the strain has the capacity of producing auxin. Research results show that the strain JP6 has potential application value in promoting tobacco growth.
Description
Technical field
The invention belongs to plant growth-promoting rhizobacteria and applied technical field thereof, be specifically related to a strain and promote there is phosphorus decomposing and producing the enterobacter cloacae of IAA function and the application of growth-promoting characteristic thereof of tobacco growing.
Background technology
Tobacco is Solanaceae (Solanaceae) Nicotiana (Nicotiana) annual herb plant.Happiness temperature, happiness light, drought-enduring, be afraid of flood, resistance to lean, need potassium more.Tobacco leaf, stem, calyx and fruit have cellulous hair, wherein contain chlorophyll in glandular hairs cell, comprehensively containing exudate that is gummy and resin, can have certain relation with the fragrance of tobacco.Tobacco petiole is not obvious or become aliform handle, and its panicle top is raw, calyx tubular, corolla funnel-form, end pink.Capsule, seed tawny.Tobacco originates in South America, because its blade is containing nicotine (Nicotine), through overmodulation, classification and processing treatment after gathering, can cigarette processed, cigar, tobacco etc., and be the important raw material of tobacco industry.There is very large problem in China's tobacco field fertilizing in recent years, is embodied in: (1) utilization rate of fertilizer is not high, and China's flue-cured tobacco utilization rate of fertilizer is lower to have data to show, some province utilization rate of fertilizer of south only about 20%.Its reason lacks further investigation to the correlation technique of utilization rate of fertilizer, particularly fundamentally do not research and solve the control of Main Tobacco-growing Regions In South fertilizer loss and the nutrient malabsorption etc. in northern cigarette district; (2) there is downtrending in various degree in vega soil fertility, and owing to lacking good culture fertility measure, crop rotation condition is in addition poor, the long-term continuous cropping of vega, and soil main nutrient elements occurs downtrending all in various degree, and soil physical property becomes bad simultaneously; (3) nutritional imbalance, the light phosphorus potassium deficiency of diazonium; (4) research is lacked to the fertilizer practice of dry farming tobacco.
Research finds can the microorganism of Promoting plant growth containing some in plant rhizosphere soil, generally has fixed nitrogen, phosphorus decomposing, releases potassium, produces the ability such as plant hormone and secretion microbiotic or at least have the bacterium, cyanobacteria etc. of one of them ability.Be referred to as plant growth-promoting rhizobacteria (PGPR), its growth-promoting functions mechanism mainly contains following several large class: provide plant nutrient substance, produce plant growth regulating substance, plant rhizosphere bioremediation agents and environment-stress conditioning agent etc.But filter out and there is several functions and efficiently microorganism strains is the target that this area is pursued always.
Tobacco absorbs macroelement nitrogen, phosphorus, potassium, the nutritive element such as moderate-element calcium, magnesium, sulphur and micronutrient boron, zinc, copper, manganese, iron.Absorbing rule is that early growth period (after transplanting to group before) is few, and mid-term (group to pinch before) absorbed dose is maximum, and later stage (after pinching) absorbed dose reduces gradually, the S-type curve of whole dietetic alimentation in vegetative period.In soil, existing phosphorus can not meet the needs of tobacco growing.Containing large amount of organic in soil, PGPR can be utilized the phosphorous organic substance decomposing in soil, produce and can be used for the phosphorus of Tobacco.Both turn avoid for tobacco supplements nutritive element the drawback too much using chemical fertilizer, such as easy contaminate environment, make soil compaction that the soil property of Tobacco Farm is damaged, the organism in loss soil, reduces soil fertility etc.And yet there are no the enterobacter cloacae that there is efficient phosphate-solubilizing simultaneously and produce IAA function and the application report promoting tobacco growing thereof.
Summary of the invention
The object of this invention is to provide the application of an Enterobacter cloacae and promotion tobacco growing thereof, this bacterial strain has phosphorus decomposing and produces the effect of growth hormone IAA.
One Enterobacter cloacae (Enterobactercloacae) JP6, this bacterial strain deposit number: CGMCCNo.9615.Depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, preservation date: on August 28th, 2014.
The culture condition of described enterobacter cloacae, adopts LB substratum: peptone 10g, yeast extract 5g, NaCl10g, distillation 1000ml, 121 DEG C of sterilizing 20min, culture temperature 37 DEG C, pH7.0.
The application of described enterobacter cloacae, for phosphorus decomposing and/or product growth hormone IAA, thus promotes the growth of plant.Described plant comprises tobacco.
Obtain bacteria suspension after strain culturing of the present invention to use as liquid microbial inoculum, bacterium Particle density>=10 of bacteria suspension
8cfu/mL.The using method of microbial inoculum: fill with root after slow seedling, every strain cigarette seedling is with microbial inoculum 1mL.
Bacterium colony and the thalline of bacterial strain of the present invention are characterized as: this bacterial strain is at LB substratum (yeast extract 5g, peptone 10g, sodium-chlor 10g, distilled water 1000mL, agar 15 ~ 20g, 121 DEG C of sterilizing 20min, pH7.0,37 DEG C) upper cultivate 48h after bacterium colony rounded, opaque, surface drying is without protuberance, faint yellow, neat in edge.Thalline is spherical, does not produce gemma, Gram-negative.
The physiological and biochemical property of this bacterial strain is: methyl red test is negative, v-p negative, Starch Hydrolysis test is positive, nitrate reduction test is positive, indole test is positive, hydrogen sulfide production test is positive, Citrate trianion utilizes test positive, glucose fermentation negative, sucrose fermentation test is negative, maltose fermentation test is negative, lactose-fermentation test is positive, mannose ferment negative, glucose carbon source utilizes test positive, the test of lactose utilization of carbon source is positive, the test of N.F,USP MANNITOL utilization of carbon source is positive, the test of sucrose utilization of carbon source is positive, the test of maltose utilization of carbon source is positive, citric acid utilization of carbon source negative, 2 ﹪ NaCl Salt tolerances are positive, 5 ﹪ NaCl Salt tolerances are positive, 7 ﹪ NaCl Salt tolerances are positive, 10 ﹪ NaCl Salt tolerances are negative, product ammonia test is positive.
The optimal culture conditions of this bacterial strain is: yeast extract 5g, peptone 10g, sodium-chlor 10g, distilled water 1000mL, agar 15 ~ 20g, pH7.0, temperature 37 DEG C.
Above bacterial strain may be used for preparing microbiobacterial agent, obtains bacteria suspension by LB culture medium culturing, as liquid microbial inoculum.
Bacterium Particle density>=10 of bacteria suspension
8cfu/mL.
The using method of microbial inoculum: fill with root every strain cigarette seedling with microbial inoculum 1mL after slow seedling.
Show after deliberation, as follows to the functional examination result of bacterial strain of the present invention: at Meng Jinna solid medium (glucose 10g, (NH
4)
2sO
40.5g, NaCl0.3g, MgSO
47H
2o0.3g, FeSO
40.03g, MnSO
4h
2o0.03g; CaCO
35.0g, KCl0.3g, Ca
3(PO
4)
225.0g, Yelkin TTS 0.3g, agar 18 ~ 20g, pH value 7.2 ~ 7.4) go up cultivation after one week, recording its colony diameter of first day d is 3.5mm, and molten phosphorus loop diameter D is 4.5mm, D/d is 1.3, within 3rd day, its colony diameter d is 4.5mm, molten phosphorus loop diameter D is 12mm, D/d be the 2.7, seven day its colony diameter d is 5mm, molten phosphorus loop diameter D is 13mm, D/d is 2.6.At inorganic phosphorus liquid nutrient medium (C
6h
12o
610.0g, (NH
4)
2sO
40.5g, NaCl0.3g, KCl0.3g, MgSO
47H
2o0.3g, FeSO
47H
2o0.03g, MnSO
44H
2o0.03g, Ca
3(PO
4)
225.0g, water 1000mL, pH7.0-7.5.) cultivate after 4 days, the content of rapid available phosphorus in nutrient solution is measured with molybdenum blue colorimetric method, thus the dissolving P capacity of reacting bacteria, recording available phosphorus contents in JP6 nutrient solution is 50.1mg/L.At R2A liquid nutrient medium (yeast powder 0.5g, Tryptones 0.5g, casamino acids 0.5g, glucose 0.5g, Zulkovsky starch 0.5g, K containing L-Trp (200mg/L)
2hPO
40.3g, MgSO
47H
2o0.05g, Sodium.alpha.-ketopropionate 0.3g.) in, 28 DEG C, 180R/min, shaking table is got 50 μ L bacteria suspensions after cultivating 4d and is dripped on whiteware plate, adds 50 μ LSalkowski color solution (50mL35%HClO simultaneously
4+ 1mL0.5mol/LFeCl
3) whiteware plate places after 30min in room temperature lucifuge and observe, color reddens expression can producing IAA, then by the OD600 value of spectrophotometry bacteria suspension, then by centrifugal for bacteria suspension 10000R/min 10min, get supernatant liquor and add equal-volume Salkowski color solution, lucifuge leaves standstill 30min makes it develop the color, measure its OD530 value, when calculating bacteria concentration OD600 value is 1, the concentration of IAA, recording IAA content in JP6 fermented liquid is 128.9mg/mL.The cigarette strain number of blade through JP6 process when potted plant 30 days is greater than contrast, maximum blade surface-area is also greater than contrast, but do not reach significant difference, through the cigarette strain plant height of JP6 process when 60 days, stem girth, maximum blade surface-area, the number of blade is all higher than contrast, through the cigarette strain plant height of JP6 process when 90 days, the number of blade reaches significant difference with contrasting, through the root of the tobacco plant of JP6 process, stem, leaf, the total fresh weight of plant is all better than control treatment, and all reach significant difference, to the root can finding out plant in biological quantitative statistics after gathering, stem, leaf dry weight and plant gross dry weight are all better than contrast, stem weight simultaneously, plant gross dry weight is all reaching significant difference.Compared with the control, root dry weight amplification is 60.8%, and the heavy amplification of stem is 54.5%, and leaf gross dry weight amplification is 10.1%, and plant weights amplification is 24.9%.Above result of study shows, JP6 bacterial strain can well promote tobacco growing, in promotion tobacco growing, have potential using value.
Enterobacter cloacae of the present invention (Enterobactercloacae) JP6.Depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, preservation date: on August 28th, 2014, deposit number: CGMCCNo.9615.
Accompanying drawing explanation
Fig. 1 is 60 days and JP6 process in 90 days and the cigarette strain plant height column diagram contrasted;
Fig. 2 is 60 days and JP6 process in 90 days and the cigarette strain stem girth column diagram contrasted;
Fig. 3 is that 30 days, 60 days and JP6 process in 90 days amass column diagram with the maximum leaf surface of cigarette strain contrasted;
Fig. 4 is 30 days, 60 days and JP6 process in 90 days and the cigarette strain number of blade column diagram contrasted;
Fig. 5 is JP6 process biomass column diagram fresh in the cigarette strain contrasted;
Fig. 6 is JP6 process and the cigarette strain dry biomass column diagram contrasted;
Fig. 7 is JP6 phosphorus decomposing design sketch.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated.
Enterobacter cloacae of the present invention (Enterobactercloacae) JP6, depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, preservation date: on August 28th, 2014, deposit number: CGMCCNo.9615.
(1) screening of CGMCCNo.9615
Process in the enrichment of beef extract-peptone liquid nutrient medium: dilution plate culture method is separated, take and pick up from level ground, Sangzhi, Hunan land owned by officials vega fresh soil 10g, be placed in the 250mL triangular flask including granulated glass sphere and 90mL sterilized water, 170r/min vibrates 30min, then by 10
-5, 10
-6, 10
-7gradient is diluted, and respectively gets 0.1mL and coats straw medium (fresh rice straw 300g, agar 25g, water 1000mL121 DEG C high pressure steam sterilization 25min.) dull and stereotyped and inorganic phosphorus substratum (glucose 10.0g, (NH
4)
2sO
40.5g, NaCl0.3g, KCl0.3g, MgSO
47H
2o0.3g, FeSO
47H
2o0.03g, MnSO
44H
2o0.03g, Ca
3(PO
4)
225.0g, water 1000mLpH7.0-7.5, agar 25g.) on flat board, wherein straw medium is coated with two parts, and a copy of it covers one deck water agar thick as far as possible.Each gradient repeats 3 times.The straw medium flat board not being stamped water agar is positioned over 15 DEG C and cultivates 5-8d, the flat board and the inorganic phosphorus culture medium flat plate that are stamped water agar are placed in 37 DEG C of cultivation 24-48h.
By bacterium colony percutaneous puncture-inoculation that water agar-straw medium grows in the test tube filling semi-solid straw medium, then above test tube, capping one deck is about the thick water agar of 1cm, and Reperfu-sion is about the thick whiteruss sealing of 2cm, manufactures complete oxygen-free environment.
Bacterium colony on the bacterium colony that straw medium under cold condition is cultivated and inorganic phosphorus substratum is inoculated on corresponding substratum again and cultivates at identical conditions.
Each process is all gone down to posterity 3 times above, chooses the bacterial isolates purification storage of fast growth.Wherein phosphorus decomposing substratum is selected the bacterial isolates containing phosphorus decomposing circle.
Adopted by the bacterial isolates that multiple sieve obtains three zoning collimation methods to carry out purifying, after purifying, the bacterium colony of the different shape of gained is verified by the condition in multiple sieve again, and record colonial morphology.
By flat board molten phosphorus circle method, molten phosphorus circle method and molybdenum blue colorimetric method, ultraviolet spectrophotometry (surveying growth hormone content), protect green method, ability of dissolving potassium assay method, measure its phosphate solubilization respectively, produce growth hormone ability, produce ability and the ability of dissolving potassium of phytokinin.Filter out a strain phosphorus decomposing can produce again the bacterial strain of growth hormone from being separated the bacterial strain that obtains, be designated as JP6.
(2) CGMCCNo.9615 strain identification
The extraction of genomic dna: will be separated after the bacterial strain streak inoculation that obtains cultivates 24h to LB solid medium in 37 DEG C, picking list bacterium colony in the test tube that LB liquid nutrient medium is housed 37 DEG C, 12h cultivated by 150r/min shaking table.Concrete grammar consults document (AusubelFM, BrentR, KingstonRE, etal.ShortProtocolsinMolecularBiology.Chichester:JohnWil ey & Sons, Inc, 1995:36-40.).The DNA obtained is dissolved in 50 μ LTE damping fluids (10mMTris-HCl, 1mMEDTA, PH=8.0), is then stored in-20 DEG C of refrigerators for subsequent use.
16SrDNA sequence amplification primer is synthesized by Shanghai biotechnology company limited.
Pcr amplification reaction system: Taq DNA polymerase 0.5 μ L, 10 × Buffer5 μ L, MgCl
23 μ L, dNTPs (10mmol/L) 1 μ L, primer 2 7F1 μ L, primer 1492R1 μ L, template 0.5 μ L, ddH
2o complements to 50 μ L.Reaction conditions: 95 DEG C of denaturation 5min; 94 DEG C of sex change 1min, 55 DEG C of annealing 1min, 72 DEG C extend 1.5min, 30 circulations; 72 DEG C extend 10min.The examining order of 16SrDNA is completed by Shanghai biotechnology company limited.
By the sequence alignment in surveyed 16SrDNA sequence and GenBank database, result shows: bacterial strain of the present invention is arranged in phylogenetic tree enterobacteria (Enterobacter.sp) branch, have nearest sibship with Enterobactercloacae, its 16SrRNA gene order similarity is 99%.
This bacterial strain is at LB substratum (yeast extract 5g, peptone 10g, sodium-chlor 10g, distilled water 1000mL, agar 15-20g, 121 DEG C of sterilizing 20min, pH7.0,37 DEG C) upper cultivate 48h after bacterium colony rounded, opaque, surface drying is without protuberance, faint yellow, neat in edge.Thalline is spherical, does not produce gemma, Gram-negative.The physiological and biochemical property of this bacterial strain is: methyl red test is negative, v-p negative, Starch Hydrolysis test is positive, nitrate reduction test is positive, indole test is positive, hydrogen sulfide production test is positive, Citrate trianion utilizes test positive, glucose fermentation negative, sucrose fermentation test is negative, maltose fermentation test is negative, lactose-fermentation test is positive, mannose ferment negative, glucose carbon source utilizes test positive, the test of lactose utilization of carbon source is positive, the test of N.F,USP MANNITOL utilization of carbon source is positive, the test of sucrose utilization of carbon source is positive, the test of maltose utilization of carbon source is positive, citric acid utilization of carbon source negative, 2 ﹪ NaCl Salt tolerances are positive, 5 ﹪ NaCl Salt tolerances are positive, 7 ﹪ NaCl Salt tolerances are positive, 10 ﹪ NaCl Salt tolerances are negative, product ammonia test is positive.By above bacterium colony, thalli morphology, physiological and biochemical property and 16SrDNA sequential analysis, identify that CGMCC9615 is enterobacter cloacae (Enterobactercloacae).
(3) phosphorus decomposing effect measuring
The present invention is screened the inoculation that obtains at Meng Jinna solid medium (glucose 10g, (NH
4)
2sO
40.5g, NaCl0.3g, MgSO
47H
2o0.3g, FeSO
40.03g, MnSO
4h
2o0.03g; CaCO
35.0g, KCl0.3g, Ca
3(PO
4)
225.0g, Yelkin TTS 0.3g, agar 18 ~ 20g, pH value 7.2 ~ 7.4) go up cultivation one week, measure its colony diameter d, molten phosphorus loop diameter D, calculate D/d.Phosphorus decomposing circle effect as shown in Figure 7, recording its colony diameter of first day d is 3.5mm, molten phosphorus loop diameter D is 4.5mm, D/d is the 1.3, three day its colony diameter d is 4.5mm, and molten phosphorus loop diameter D is 12mm, D/d is 2.7, within 7th day, its colony diameter d is 5mm, and molten phosphorus loop diameter D is 13mm, D/d is 2.6.By JP6 at inorganic phosphorus liquid nutrient medium (glucose 10.0g, (NH
4)
2sO
40.5g, NaCl0.3g, KCl0.3g, MgSO
47H
2o0.3g, FeSO
47H
2o0.03g, MnSO
44H
2o0.03g, Ca
3(PO
4)
225.0g, water 1000mL, pH7.0-7.5.) middle cultivation is after 4 days, molybdenum blue colorimetric method measures the content of rapid available phosphorus in nutrient solution, thus the dissolving P capacity of reacting bacteria.Recording available phosphorus contents in JP6 nutrient solution is 50.1mg/L.
(4) produce growth hormone ability to measure
Received by JP6 in the R2A liquid nutrient medium containing L-Trp (200mg/L), 28 DEG C, 180R/min, 4d cultivated by shaking table.Getting 50 μ L bacteria suspensions drips on whiteware plate, adds 50 μ LSalkowski color solution (50mL35%HClO simultaneously
4+ 1mL0.5mol/LFeCl
3).To the color solution of 50 μ L50mg/LIAA be added as positive control.Whiteware plate is observed after room temperature lucifuge places 30min, and color reddens expression can producing IAA.
Carry out quantitative assay to the bacterium of the producing IAA that primary dcreening operation obtains, culture condition is the same.First by the OD600 value of spectrophotometry bacteria suspension, then by centrifugal for bacteria suspension 10000R/min 10min, get supernatant liquor and add equal-volume Salkowski color solution, lucifuge leaves standstill 30min makes it develop the color, and measures its OD530 value.When calculating bacteria concentration OD600 value is 1, the concentration of IAA.Recording IAA content in JP6 fermented liquid is 128.9mg/mL.
(5) pot experiment
First be nursery, select the tobacco seed of same kind to carry out nursery.Stalwartness is selected in nursery for 30 days afterwards, the cigarette seedling that anosis, growing way is consistent is transplanted, and the strain of every potted plant cigarette 1, mainly all imbeds cigarette seedling cane in soil, water the water of equivalent after transplanting immediately.
Then be process, one group of blank, one group adds JP6 microbial inoculum.Cultivate to obtain bacteria suspension of the present invention (bacterium Particle density cfu>=10 of bacteria suspension
8individual/mL), use as liquid microbial inoculum, fill with root after slow seedling, every strain cigarette seedling is with microbial inoculum 1mL.
Pendulum basin: for reducing because the difference of booth different directions temperature is on the impact of experimental result, basin should be put along with booth vertical direction.
Soil humidity is noted in the tobacco growing phase, then need moisturizing to 80% (moisturizing can be determined according to the growth of cigarette strain and lack of water situation, but must ensure that the irrigation amount of every basin and soil humidity are consistent) of maximum water holding capacity lower than after soil relative water content 50%.
Every 10 days investigation numbers of blade after transplanting, plant height, stem girth, leaf look, maximum leaf length and width, growing way, residing breeding time etc.When finding that between process, growing way appearance has a notable difference in potted plant process, contrast is taken pictures together with process pendulum, the root system sampling cigarette strain is carried out contrast simultaneously and take pictures.
Result is as shown in table 1, table 2, table 3, table 4, table 5
Table 1.30 day JP6 is on the impact of tobacco agronomy character
Note: data bit mean number ± standard deviation in table.The significance of difference (p<0.05) is shown with lowercase alphabet different after column data, lower same.
Table 2.60 day JP6 is on the impact of tobacco agronomy character
Table 3.90 day JP6 is on the impact of tobacco agronomy character
Table 4.JP6 is on the impact of cigarette strain fresh weight
Table 5.JP6 is on the impact of cigarette strain dry weight
In conjunction with the accompanying drawings and embodiments the specific embodiment of the present invention is described although above-mentioned; but not limiting the scope of the invention; one of ordinary skill in the art should be understood that; on the basis of technical scheme of the present invention, those skilled in the art do not need to pay various amendment or distortion that creative work can make still within protection scope of the present invention.
Claims (8)
1. an Enterobacter cloacae (Enterobactercloacae) JP6, this bacterial strain deposit number: CGMCCNo.9615.
2. the culture condition of enterobacter cloacae according to claim 1, is characterized in that, adopts LB substratum: peptone 10g, yeast extract 5g, NaCl10g, distillation 1000ml, 121 DEG C of sterilizing 20min, culture temperature 37 DEG C, pH7.0.
3. the application of enterobacter cloacae according to claim 1, is characterized in that, for phosphorus decomposing and/or product growth hormone IAA.
4. the application of enterobacter cloacae according to claim 3, is characterized in that, for phosphorus decomposing and/or the growth promoting plant after producing growth hormone IAA.
5. the application of enterobacter cloacae according to claim 4, is characterized in that, described plant comprises tobacco.
6. the application of the enterobacter cloacae according to claim 3 or 4 or 5, is characterized in that, obtains bacteria suspension and use as liquid microbial inoculum after strain culturing.
7. the application of enterobacter cloacae according to claim 6, is characterized in that, bacterium Particle density>=10 of bacteria suspension
8cfu/mL.
8. the application of enterobacter cloacae according to claim 7, is characterized in that, the using method of microbial inoculum: fill with root after slow seedling, every strain cigarette seedling is with microbial inoculum 1mL.
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| CN105695359A (en) * | 2016-03-20 | 2016-06-22 | 贵州省草业研究所 | Efficient phosphate solubilizing bacteria capable of promoting growth of overground tissue of trifolium repens and application of phosphate solubilizing bacteria |
| CN105838750A (en) * | 2016-03-29 | 2016-08-10 | 四川省兰月科技有限公司 | Application of enterobacteria LY6 in fermentation production of indoleacetic acid and application of fermenting liquid in crop rooting and growth promotion |
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| CN106318889A (en) * | 2016-08-31 | 2017-01-11 | 云南磷化集团有限公司 | Enterobacter strain capable of dissolving phosphorus and application of enterobacter strain |
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| CN108795797A (en) * | 2018-05-24 | 2018-11-13 | 珠海市现代农业发展中心(珠海市金湾区台湾农民创业园管理委员会、珠海市农渔业科研与推广中心) | Raw enterobacter cloacae and its application in one plant of maize root system |
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| CN111808783A (en) * | 2020-08-03 | 2020-10-23 | 山东佐田氏生物科技有限公司 | Saline-alkali tolerant enterobacter cloacae and production method and application of viable bacteria preparation thereof |
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