CN105695359B - Efficient phosphate solubilizing bacterium capable of promoting growth of overground tissues of Trifolium repens and application of efficient phosphate solubilizing bacterium - Google Patents
Efficient phosphate solubilizing bacterium capable of promoting growth of overground tissues of Trifolium repens and application of efficient phosphate solubilizing bacterium Download PDFInfo
- Publication number
- CN105695359B CN105695359B CN201610155718.7A CN201610155718A CN105695359B CN 105695359 B CN105695359 B CN 105695359B CN 201610155718 A CN201610155718 A CN 201610155718A CN 105695359 B CN105695359 B CN 105695359B
- Authority
- CN
- China
- Prior art keywords
- phosphate solubilizing
- overground
- strain
- growth
- tissues
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Virology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a high-efficiency phosphate solubilizing bacterium capable of promoting overground tissue growth of trifolium repens, which is classified and named as Enterobacter cloacae (Enterobacter cloacae); strain number BS 1; is preserved in the general microbiological center of China Committee for culture Collection of microorganisms of the institute of microbiology of China academy of sciences, with the preservation number of CGMCC No.11788 and the preservation date of 2015, 12 months and 04 days. After the efficient phosphate solubilizing bacteria BS1 are inoculated to the white clover, the efficient phosphate solubilizing bacteria BS1 have a very remarkable growth promoting effect on the overground tissues of the white clover.
Description
Technical Field
The invention relates to the technical field of microorganisms, in particular to high-efficiency phosphate solubilizing bacteria capable of promoting growth of overground tissues of Trifolium repens and application thereof.
Background
The phosphorus element is taken as a nutrient element necessary for the growth of the plants, participates in the metabolic activity of the plants, and has the effects of enhancing the stress resistance, promoting the growth and the like on the plants. Researches show that available phosphorus in soil hardly meets the requirement of plant growth on phosphorus, and a large part of phosphorus in phosphate fertilizer is combined with Fe2+、Fe3+、Ca2+And Al3+The cations are combined to form a phosphate compound which is difficult to be absorbed by plants and is difficult to dissolve[1-2]. Therefore, how to improve the utilization rate of the phosphorus element by the plants has important significance for the growth of the plants and the improvement of the soil environment.
The phosphorus-dissolving bacteria serving as beneficial microorganisms of plant rhizosphere can convert insoluble phosphorus into phosphorus elements which can be absorbed by plants, and has important significance for improving soil environment and promoting plant growth. On one hand, some high-efficiency phosphate solubilizing bacteria have the capability of secreting phytohormones so as to promote the growth of plants; on the other hand, the higher phosphorus dissolving capacity of the fertilizer can promote the absorption of plants to inorganic phosphorus and organic phosphorus, thereby meeting the requirements of plants on phosphorus elements and further promoting the growth of plants. Therefore, the method has important significance for the screening of the plant rhizosphere phosphate solubilizing bacteria and the research of the growth promoting effect of the plant rhizosphere phosphate solubilizing bacteria.
Disclosure of Invention
The invention aims to provide the efficient phosphate solubilizing bacteria capable of promoting the growth of the overground tissues of the trifoliate repens, so that the efficient phosphate solubilizing bacteria can achieve a remarkable promoting effect on the overground tissues of the trifoliate repens by inoculating the trifoliate repens.
The efficient phosphate solubilizing bacteria capable of promoting overground tissue growth of the trifolium repens is obtained by separating the efficient phosphate solubilizing bacteria from the rhizosphere of the trifolium repens, and measuring the calcium phosphate dissolving capacity of the strain by using a molybdenum blue colorimetric method, wherein the phosphorus dissolving capacity is 424.85 mu g.mL-1Growth-promoting experimental research shows that the strain promotes the growth of the overground part of the white clover.
According to morphological feature observation and physiological and biochemical test results, the Bergey bacteria identification manual and the common bacteria system identification manual are found, so that the efficient phosphate solubilizing bacteria are Enterobacter cloacae, and the strain is named as BS 1.
The strain is obtained by separating the rhizosphere soil sample of trifolium repens cultivated in the single mountain county of Guizhou province in 10 months in 2010 by the inventor. The strain is preserved in the common microorganism center of China Committee for culture Collection of microorganisms of China institute of microbiology, China academy of sciences, with the preservation number of CGMCC No.11788 and the preservation address of: western road No.1, north west city of township, beijing, institute of microbiology, china academy of sciences; the preservation date is 2015, 12 months and 04 days.
The strain BS1 can be used for inoculating the white clover and can remarkably promote the growth of the overground tissues of the white clover.
The method for inoculating the efficient phosphate solubilizing bacteria specifically comprises the following steps:
(1) activating and culturing the strain, and preservingThe strain was streaked on a PKO medium, cultured at 28 ℃ for 3 days, and repeatedly cultured 3 times. The PKO culture medium is prepared as follows: ca3(PO4)23.0g、(NH4)2SO40.5g、KCl 0.2g、MnSO40.03g、FeSO4·7H2O 0.003g、NaCl 0.2g、MgSO4·7H20.03g of O, 10g of glucose, 0.5g of yeast powder, 15-20g of agar and 1000ml of distilled water, and the pH value is 6.8-7.2.
(2) When no bacteria colony grows out in the culture medium, selecting a single bacterial colony, inoculating the single bacterial colony into 0.5ml of bacterial suspension in 50ml of LB culture medium, and performing shake culture at 28 ℃ for 3-5 days. The LB culture medium is prepared as follows: 10.0g of peptone, 6.0g of NaCl, 5.0g of yeast powder, 1000ml of distilled water and pH 6.8-7.2.
(3) Sterilizing Trifolium repens seeds with 0.1% mercuric chloride for 5min, sterilizing with 75% alcohol for 3min, and washing with sterile water.
(4) Control group: soaking the sterilized seeds in sterile water for 3 d; test groups: and soaking the disinfected seeds in the high-efficiency phosphorus-dissolving bacterium liquid for 3 d.
(5) When the seeds have white buds, transplanting the seeds into a test tube bacterium culture medium, and culturing for 40d under the condition of illumination for 16h at 25 ℃, wherein the test tube bacterium culture medium is prepared as follows: ca (NO)3)20.945g、KNO30.506g、NH4NO30.08g、MgSO40.493g、Ca3(PO4)20.62g, 2.5ml of iron salt solution, 2ml of trace element solution, 4.0-6.0g of agar, 1000ml of distilled water and pH 6.0; the ferric salt solution is prepared by the following steps: FeSO4·7H2O2.78g, disodium EDTA 3.73g and distilled water 500 ml; the trace element liquid is prepared by the following steps: KI 0.0215g, MnSO41.15g、NaMoO4·2H2O 0.00125g、CuSO40.00125g and 500ml of distilled water.
(6) The inoculated strain BS1 has obvious promotion effect on the growth of the white clover, and the fresh weight and the dry weight on the ground are both obviously higher than those of a plant of an uninoculated strain BS1 (p is less than 0.01).
Drawings
FIG. 1 is a characteristic diagram of a target strain phosphorus-solubilizing circle provided in the examples of the present invention;
FIG. 2 is a characteristic diagram of colonies provided by an embodiment of the present invention
FIG. 3 is a characteristic diagram of a colony blood plate provided in an embodiment of the present invention
FIG. 4 is a characteristic diagram of microscopic examination of strain BS1 provided by the embodiment of the invention
FIG. 5 is a schematic diagram of growth promoting effect of strain BS1 on Trifolium pratense provided by the embodiment of the invention
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings and examples.
Example 1: obtaining of high-efficiency phosphate solubilizing bacterium strain BS1
The separation and screening process of the high-efficiency phosphate solubilizing bacterium strain BS1 is as follows:
(1) separation of high-efficiency phosphate solubilizing bacterium strain BS 1:
the soil is taken from the single mountain county of Guizhou province in 10 months in 2010, and rhizosphere samples of the cultivated trifolium repens are taken by a 5-point method, wherein the samples are located between 106 degrees 12 'to 108 degrees 18' of the east longitude and 25 degrees 04 'to 27 degrees 29' of the north latitude, the average altitude is 997m, the average annual temperature is 13.6-19.6 ℃, and the average annual rainfall is 1100-1400 mm. The soil type is yellow loam, and the main components are as follows: 0.218 g/kg-1 of total nitrogen, 156.98 mg/kg-1 of hydrolyzed nitrogen, 15.02 mg/kg-1 of available phosphorus, 10.20 mg/kg-1 of quick-acting potassium, 1.695 g/kg-1 of organic matters and pH 5.97. The specific separation method of the efficient phosphate solubilizing bacteria is disclosed in reference documents[4]。
(2) Primary screening of the high-efficiency phosphate solubilizing bacterium strain BS 1:
the primary screening and the phosphorus dissolving capacity determination of the high-efficiency phosphorus dissolving bacterium strain BS1 are carried out by applying an inorganic phosphorus culture medium containing calcium phosphate[5]LB culture medium for purifying, activating and preserving strain[6]. Activating the separated and purified strains for 2-3 times, selecting a strain drop point by using a sterile toothpick, inoculating the strain drop point on a PKO culture medium, placing each strain on four points on a flat plate, and culturing for 15 days at the constant temperature of 28 ℃. The size of the clear circles (D) and the size of colonies (D) appearing on the strains were observed and recorded at 5 th, 10 th and 15 th days, respectively, and the strains were preliminarily selected according to the D/D sizes (Table 1). The strains all show stronger phosphorus dissolving capacity on phosphorus-containing plate culture medium, and can dissolve phosphorus in the culture medium to generate larger transparent circles (figure 1, figure 2))。
TABLE 1 determination of P-lysing bacteria of Trifolium pratense D/D value
(3) Rescreening of the high-efficiency phosphate solubilizing bacterium strain BS 1:
50mL of sterilized calcium phosphate inorganic phosphorus culture medium is filled into a 150mL triangular flask, 1mL of bacterial suspension for culturing 1-2d of the bacterial strain is respectively inoculated, the bacterial suspension is repeatedly cultured for 3 times by each bacterial strain without inoculation control, and the bacterial strain is subjected to shake culture for 7d on a constant-temperature shaking table at the temperature of 28 ℃ and at the speed of 150 r/min.
② 8d centrifuging the culture solution at 10000r/min for 10min, determining the phosphorus content by molybdenum-antimony colorimetry[7]The amount of dissolved phosphorus is subtracted from the control value, expressed as P2O5The phosphorus-dissolving amount of the obtained strain is 424.85 +/-43.03 mu g.mL < -1 >;
③ taking 5mL of supernatant, titrating by 0.1mol/LNaOH solution (phenolphthalein is used as indicator), calculating the organic acid secretion amount of the strain to be 12.00 +/-1.15 mmol.L-1[8]。
Example 2: the identification process of the efficient phosphate solubilizing bacterium strain BS1 is as follows:
morphological identification of the efficient phosphate solubilizing bacterium strain BS 1;
and (4) marking a single colony, observing the morphology of the colony, wherein the surface of the colony is smooth and opaque, and the edge is neat. Selecting strains to be identified, respectively scribing on nutrient agar plates, culturing for 48 hours, then carrying out liquid culture, and inoculating the selected strains in a nutrient broth culture base to culture for 48 hours, wherein the liquid culture medium is diffusively turbid. Microscopic examination revealed that the strain was Brevibacterium, smooth in surface, and had perisomatic flagella (FIG. 4).
Strain BS1 has the following properties:
1. morphological characteristics of colonies:
and (4) single colony is scratched, the colony morphology is observed, the colony surface is smooth and opaque, and the edge is neat. Selecting strains to be identified, respectively streaking the strains on nutrient agar plates, culturing for 48 hours, then carrying out liquid culture, and inoculating the selected strains in a nutrient broth culture base to culture for 48 hours, wherein the liquid culture medium is in a diffusion turbidity state. Microscopic examination revealed that the strain was Brevibacterium, smooth in surface, and had perisomatic flagella (FIG. 4).
2. Physiological and biochemical identification characteristics:
(1) gram staining: negative;
(2) anaerobic growth test: positive;
(3) glucose oxidation fermentation experiment: opening the tube to produce acid and turn yellow: positive; closed tube acidogenic yellowing: positive; gas production: positive; and (3) movement: positive;
(4) contacting with enzyme: positive;
(5) an oxidase: negative;
(6) and (3) urease test: negative;
(7) V-P determination: positive;
(8) liquefaction of gelatin: negative;
(9) nitrate reduction: positive;
(10) indole: negative;
(11) citrate salt: positive;
(12) malonic acid: positive;
(13) hydrogen sulfide: negative;
(14) and (3) ONPG measurement: positive;
(15) lysine decarboxylase: negative;
(16) arginine hydrolase arginine: positive;
(17) arginine hydrolase arginine control: negative;
3.16SrDNA sequence analysis:
extracting 15ml of DNA of fresh bacteria, carrying out PCR amplification by using a primer combination F27 and R1492, wherein the PCR reaction program comprises 90 ℃, 5min, 94 ℃, 1min, 56 ℃, 1min, 72 ℃, 2min, 72 ℃, 7min and 30 cycles, and the 16SrDNA sequence of the strain BS1 is obtained by recovering and sequencing, wherein the length is 1465bp, and is shown in a sequence table 1. And (3) carrying out online comparison analysis on the sequencing result and other different strain sequences by applying NCBI-Blast software. According to the comparison result, the homology of Leclercia adecaboxgata, Enterobacter ludwigii and Enterobacter asburae of the bacterial bead BS1 reaches 99.5 percent, 99.2 percent and 99.1 percent respectively; the homology with Enterobacter sp is 99.5%.
Analysis of a full-automatic identification system of Blolog microorganisms:
the results of identifying the phosphate solubilizing bacteria BS1 by a Biolog bacteria identifier show that the phosphate solubilizing bacteria belong to Enterobacter cloacae ss dispovens, and the SIM value is 0.610.
Example 3: the growth promotion effect of the high-efficiency phosphate solubilizing bacterium strain BS1 on the overground plant of the Trifolium pratense is as follows:
1. inoculation of the strains:
activating and culturing the strain, streaking the preserved strain on a PKO culture medium, culturing at 28 ℃ for 3 days, and repeatedly culturing for 3 times. When no bacteria colony grows out in the culture medium, selecting a single bacterial colony, inoculating the single bacterial colony into 0.5ml of bacterial suspension in 50ml of LB culture medium, and performing shake culture at 28 ℃ for 3-5 days. Sterilizing Trifolium repens seeds with 0.1% mercuric chloride for 5min, sterilizing with 75% alcohol for 3min, and washing with sterile water. Control group: soaking 5 sterilized seeds in sterile water for 3 days; test groups: and 5 disinfected seeds are soaked in the high-efficiency phosphorus-dissolving bacterium liquid for 3 d. When the seeds have white buds, the seeds are transplanted into a test tube bacterium culture medium and cultured for 40 days under the condition of illumination for 16h at 25 ℃.
2. And (3) determination of the white clover growth indexes:
after cultivation for 40 days, the Trifolium repens plants were removed and the fresh weight on the ground was measured. The plants were then dried and the above-ground and underground dry weights of the trifolium repens plants were measured (table 2).
TABLE 2 determination of Trifolium repens growth index
3. The growth promotion effect of the efficient phosphorus-dissolving bacteria on the overground plant of the Trifolium pratense is as follows:
under the test condition, the growth-promoting bacteria influence the growth-promoting effect of the white clover. The result shows that the strain has obvious growth promoting effect on the overground weight of the trifolium repens, and the overground fresh weight and the overground dry weight of the trifolium repens inoculated with the growth promoting bacteria are both obviously higher than those of plants not inoculated with the growth promoting bacteria (p is less than 0.01).
Reference documents:
[1] ma Chi, Yangji, gold character, development and application of environmental biological agent [ M ]. Beijing, chemical industry Press, 78-91.
[2] Zhao Xiaorong, enlightening Lin, research progress of microbial phosphorus removal [ J ] soil fertilizer, 2001, (3) 7-11.
[4] Permissive, Zhenghongyuan, handbook of methods for analyzing soil microorganisms, Beijing, agricultural Press, 1986,187-189.
[5] The number and population distribution of phosphate-solubilizing bacteria in four different ecosystems of Linqimei, Zhao Xiaorong and Sun Yanxin are 2000,9(1):34-37.
[6] Study on culture conditions of efficient PGPR bacteria at rhizosphere of oat in alpine region [ J ]. proceedings of university of Gansu agriculture, 2004,39(3): 316-.
[7] The separation and purification of phosphate-solubilizing bacteria in West lake sediment and the phosphate-solubilizing capability [ J ] of the phosphate-solubilizing bacteria, applied ecology report, 2006.17(11):2112-2116.
[8] Wan , Kanglihua, Liao articles, etc. research on isolation, culture and phosphate-solubilizing ability of rhizosphere phosphate-solubilizing bacteria in mangrove forest [ J ] scientific research in forestry, 2004,17(1):89-94.
Of course, the above is only a specific application example of the present invention, and other embodiments of the present invention are also within the scope of the present invention.
Sequence listing
EQUENCE LISTING
Sequence listing
<110> research institute of grass and grass in Guizhou province
<120> efficient phosphate solubilizing bacterium capable of promoting growth of overground tissues of Trifolium repens and application thereof
<130> nm:
<160> 1
<170> PatentIn version
<210> SEQ ID NO： 1
<211> 1465
<212> DNA
<213> Enterobacter cloacae (Enteuactor cloacae) BS1
<220>
<221> rDNA
<400> 1
GCATGCGCAG CTACACATGC AGTCGAGCGG TAGCACAGGG AGCTTGCTCC TGGGTGACGA 60
GCGGCGGACG GGTGAGTAAT GTCTGGGAAA CTGCCTGATG GAGGGGGATA ACTACTGGAA 120
ACGGTAGCTA ATACCGCATA ACGTCGCAAG ACCAAAGAGG GGGACCTTCG GGCCTCTTGC 180
CATCAGATGT GCCCAGATGG GATTAGCTAG TGAGGTGGGG TAACGGCTCA CCTAGGCGAC 240
ACGATCCCTA GCTGGTCTGA GAGGATAGAC CAGCCACACT GGAACTGAGA CACGGTCCAG 300
ACTCCTACGG GAGGCAGCAG TGGGGAATAT TGCACCAATG GGCGCAAGCC TGATGCAGCC 360
ATGCCGCGTG TATGAAGAAG GCCTTCGGGT TGTAAAGTAC TTTCAGCGGG GAGGAAGGTG 420
TTGAGGTTAA TAACCTCAGC AATTGACGTT ACCCGCAGAA GAAGCACCGG CTAACTCCGT 480
GCCAGCAGCC GCGGTAATAC GGAGGGTGCA AGCGTTAATC GGAATTACTG GGCGTAAAGC 540
GCACGCAGGC GGTCTGTCAA GTCGGATGTG AAATCCCCGG GCTCAACCTG GGAACTGCAT 600
TCGAAACTGG CAGGCTAGAG TCTTGTAGAG GGGGGTAGAA TTCCAGGTGT AGCGGTGAAA 660
TGCGTAGAGA TCTGGAGGAA TACCGGTGGC GAAGGCGGCC CCCTGGACAA AGACTGACGC 720
TCAGGTGCGA AAGCGTGGGG AGCAAACAGG ATTAGATACC CTGGTAGTCC ACGCCGTAAA 780
CGATGTCGAC TTGGAGGTTG TTCCCTTGAG GAGTGGCTTC CGGAGCTAAC GCGTTAAGTC 840
GACCGCCTGG GGAGTACGGC CGCAAGGTTA AAACTCAAAT GAATTGACGG GGGCCCGCAC 900
AAGCGGTGGA GCATGTGGTT TAATTCGATG CAACGCGAAG AACCTTACCT ACTCTTGACA 960
TCCAGAGAAC TTAGCAGAGA TGCTTTGGTG CCTTCGGGAA CTCTGAGACA GGTGCTGCAT 1020
GGCTGTCGTC AGCTCGTGTT GTGAAATGTT GGGTTAAGTC CCGCAACGAG CGCAACCCTT 1080
ATCCTTTGTT GCCAGCGGTT CGGCCGGGAA CTCAAAGGAG ACTGCCAGTG ATAAACTGAG 1140
AGTGGATAGA GTCAGGTCGG ATGCCTTACG TAGGCTCACA GTGCATCATG GCCCTTACGA 1200
GTAGGGCTAC GAGGCACGTG CTACAATGGC GCATACAAAG AGAAGCGACC TCGCGAGAGC 1260
AAGCGGACCT CATAAAGTGC GTCGTAGTCC GGATTGGAGT CTGCAACTCG ACTCCATGAA 1320
GTCGGAATCG CTAGTAATCG TAGATCAGAA TGCTACGGTG AATACGTTCC CGGGCCTTGT 1380
ACACACCGCC CGTCACACCA TGGGAGTGGG TTGCAAAAGA AGTAGGTAGC TTAACCTTCG 1440
GGAGGGCGCT ACCACTTTGA TCCCC 1465
Claims (4)
1. A high-efficiency phosphate solubilizing bacterium capable of promoting the growth of overground tissues of the white clover is classified and named as Enterobacter cloacae; strain number BS 1; is preserved in the general microbiological center of China Committee for culture Collection of microorganisms of the institute of microbiology of China academy of sciences, with the preservation number of CGMCC No.11788 and the preservation date of 2015, 12 months and 04 days.
2. A biological agent comprising the strain of claim 1 having the strain number BS 1.
3. The use of the phosphate solubilizing bacterium of claim 1 for promoting the growth of tissues on the Trifolium repens.
4. The use of the efficient phosphate solubilizing bacteria as claimed in claim 3, wherein said bacteria are selected from the group consisting of: after the efficient phosphate solubilizing bacteria BS1 are inoculated to the white clover, the efficient phosphate solubilizing bacteria BS1 have a very remarkable growth promoting effect on the overground tissues of the white clover.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610155718.7A CN105695359B (en) | 2016-03-20 | 2016-03-20 | Efficient phosphate solubilizing bacterium capable of promoting growth of overground tissues of Trifolium repens and application of efficient phosphate solubilizing bacterium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610155718.7A CN105695359B (en) | 2016-03-20 | 2016-03-20 | Efficient phosphate solubilizing bacterium capable of promoting growth of overground tissues of Trifolium repens and application of efficient phosphate solubilizing bacterium |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105695359A CN105695359A (en) | 2016-06-22 |
| CN105695359B true CN105695359B (en) | 2021-08-31 |
Family
ID=56231328
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610155718.7A Expired - Fee Related CN105695359B (en) | 2016-03-20 | 2016-03-20 | Efficient phosphate solubilizing bacterium capable of promoting growth of overground tissues of Trifolium repens and application of efficient phosphate solubilizing bacterium |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105695359B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003250526A (en) * | 2002-03-04 | 2003-09-09 | Towa Corp:Kk | New microorganism, method for degrading rubber with the same, and apparatus for degrading rubber with the same microorganism |
| CN102787088A (en) * | 2012-08-07 | 2012-11-21 | 哈尔滨师范大学 | Enterobacter cloacae and application of enterobacter cloacae |
| CN104212747A (en) * | 2014-09-17 | 2014-12-17 | 黑龙江省科学院微生物研究所 | Enterobacter cloacae having phosphorus solubilizing function and used for degrading imazethapyr |
| CN105255769A (en) * | 2015-11-03 | 2016-01-20 | 湖南中烟工业有限责任公司 | Enterobacter cloacae and application thereof |
-
2016
- 2016-03-20 CN CN201610155718.7A patent/CN105695359B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003250526A (en) * | 2002-03-04 | 2003-09-09 | Towa Corp:Kk | New microorganism, method for degrading rubber with the same, and apparatus for degrading rubber with the same microorganism |
| CN102787088A (en) * | 2012-08-07 | 2012-11-21 | 哈尔滨师范大学 | Enterobacter cloacae and application of enterobacter cloacae |
| CN104212747A (en) * | 2014-09-17 | 2014-12-17 | 黑龙江省科学院微生物研究所 | Enterobacter cloacae having phosphorus solubilizing function and used for degrading imazethapyr |
| CN105255769A (en) * | 2015-11-03 | 2016-01-20 | 湖南中烟工业有限责任公司 | Enterobacter cloacae and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 一株烤烟根际解磷细菌的鉴定及其在烤烟生产中的应用;李晓举;《河南农业科学》;20111231;第40卷(第6期);第66-70页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105695359A (en) | 2016-06-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107236690B (en) | Bacillus pumilus RP01 and application thereof | |
| Akhter et al. | Isolation and characterization of salinity tolerant Azotobacter sp | |
| CN111676164B (en) | Phosphate solubilizing bacterium RP2, and fermentation product, microbial inoculum and application thereof | |
| Kamako et al. | Establishment of axenic endosymbiotic strains of Japanese Paramecium bursaria and the utilization of carbohydrate and nitrogen compounds by the isolated algae | |
| CN113801817B (en) | Phosphate solubilizing bacteria 3-1 and application thereof in dissolving phosphate | |
| CN109097305B (en) | Rhizobium and application thereof | |
| Sulaiman et al. | Isolation and identification of Azospirillum and Azotobacter species from Acacia spp. at Riyadh, Saudi Arabia | |
| CN117106614B (en) | Rhizosphere bacterium pseudomonas solanacearum YIM B08402, microbial agent and application thereof | |
| CN111084011B (en) | Endophytic fungus capable of enhancing activity of casuarina equisetifolia acid phosphatase in low-phosphorus environment | |
| CN117264838A (en) | Pseudomonas with growth promoting function on plant lead stress and application thereof | |
| CN110343619B (en) | Endophytic fungus capable of promoting height and ground diameter growth of schima superba seedlings in low-phosphorus environment | |
| CN102321548B (en) | Rhizobium sp. T3 and applications thereof in microbial degradation hydrogen sulfide | |
| CN114752538B (en) | Oil tea endophyte with soil improvement function and application thereof | |
| Kuzmina et al. | Characterization of the novel plant growth-stimulating strain Advenella kashmirensis IB-K1 and evaluation of its efficiency in saline soil | |
| CN109423456B (en) | Azotobacter chroococcum as well as identification method and application thereof | |
| CN107099486B (en) | Bacillus amyloliquefaciens GN03 and application thereof | |
| CN105695359B (en) | Efficient phosphate solubilizing bacterium capable of promoting growth of overground tissues of Trifolium repens and application of efficient phosphate solubilizing bacterium | |
| CN116064307A (en) | Burkholderia strain capable of efficiently dissolving phosphorus and application thereof | |
| CN110261267B (en) | Detection method of photosynthetic bacteria preparation product for fishing | |
| CN111979159A (en) | Phosphate solubilizing bacterium agent and preparation method and application thereof | |
| CN116590195B (en) | Pseudomonas, microbial preparation and application thereof | |
| CN111718874B (en) | Phosphorus-solubilizing bacterium RP22, fermentation product, microbial inoculum and application thereof | |
| CN109593673A (en) | A kind of Flavobacterium JX-1 and its application in waste water control | |
| CN109439587A (en) | One plant of ocean fixed nitrogen series bacillus and its application | |
| CN114410545B (en) | Paracoccus GN-9 and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210831 |