CN109593673A - A kind of Flavobacterium JX-1 and its application in waste water control - Google Patents
A kind of Flavobacterium JX-1 and its application in waste water control Download PDFInfo
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- CN109593673A CN109593673A CN201811533538.3A CN201811533538A CN109593673A CN 109593673 A CN109593673 A CN 109593673A CN 201811533538 A CN201811533538 A CN 201811533538A CN 109593673 A CN109593673 A CN 109593673A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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Abstract
The present invention provide a kind of novel bacterial Flavobacterium (Flavobacterium sp.) JX-1 and its application in waste water control.The present invention by separated in Poyang Lake dry season water body one plant of Flavobacterium (Flavobacterium sp.) JX-1 GDMCC No.60424, it can be degraded organic nitrogen using the strain isolated, nitrogenous effluent is purified using the bacterial strain ammonification ability, is with a wide range of applications for microorganism fungus kind applied technical field.
Description
Technical field
The present invention relates to microorganism fungus kind and its technical field of application, be specifically related to a kind of new Flavobacterium JX-1 and
Its technical field applied in waste water control.
Background technique
Water pollution has become mankind's urgent problem, under the influence of mankind's activity, biology required nitrogen, phosphorus etc.
Nutriment largely enters lake slow flow water bodies, causes water eutrophication phenomenon, and eutrophication causes function of water body decline, water
The catastrophic effects such as matter deterioration, planktonic organism mortality.Nitrogen is the required basic nutrition element of biological life activity, and
Cause one of the key element of water eutrophication, Nitrogen Cycling occupies extremely important status, China in water nutrition circulation
Water body polluted by nitrogen is very serious, it not only constrains the utilizability of water resource, and directly affect the life health of the mankind with
The good development of social economy.
Macromolecular organic nitrogen-containing object is exactly released the micro- of ammoniacal nitrogen by oxidation, hydrolysis, reduction by amonifying bacteria
Biology belongs to heterotrophicy bacteria, is the tie for connecting organic nitrogen and inorganic nitrogen in Nitrogen Cycling.Organic nitrogen is the important set of water body
Point, amonifying bacteria, by ammoniation, decomposes organic nitrogen compound and generates ammonia nitrogen, this is played in water ecosystem in water body
Very important effect.Therefore, the research of the screening and its biological characteristics of carrying out amonifying bacteria new strains has important reason
Value and practice significance.
Summary of the invention
This hair provides a kind of novel bacterial Flavobacterium (Flavobacterium sp.) JX-1 and its answering in waste water control
With.The present invention in Poyang Lake dry season water body by separating one plant of Flavobacterium (Flavobacterium sp.) JX-
1GDMCC No.60424 can be degraded organic nitrogen using the strain isolated, using the bacterial strain ammonification ability to nitrogenous effluent into
Row purification, is with a wide range of applications for microorganism fungus kind applied technical field.
The main technical schemes that the present invention uses:
Invention carries out amonifying bacteria and denitrifying bacteria according to the particularity of Poyang Lake geographical environment from Poyang Lake
Culture, separation and screening, obtain a collection of bacterium, and therefrom separate the Flavobacterium that one plant of number is JX-1
(Flavobacterium sp.), through the analysis of 16S rDNA sequence, Phylogenetic Analysis, Microbiological Characteristics analysis and fatty acid
Composition analysis is as a result, JX-1 bacterial strain belongs to Flavobacterium (Flavobacterium sp.), the bacterial strain optimum growing condition are as follows: temperature
10-37 DEG C of degree can be grown, and optimum growth temperature is 28 DEG C, can be grown within the scope of pH value 5.0-11.0, optimal pH 7.0, can be
It is grown under the conditions of NaCl concentration 0-2.0%, most suitable NaCl concentration is 1%, incubation time 24-48h, and culture medium is R2A culture
Base.Morphology, physiological and biochemical test are carried out to bacterial strain referring to " primary Jie Shi systematic bacteriology identification handbook " (the 9th edition) etc., determined
Member in the Flavobacterium that JX-1 bacterial strain is, but have the characteristics that it is different from common Flavobacterium member's strain, have one
The characteristic of a little novel bacterials.
Further, the present invention carries out gene sequencing by the strain for being JX-1 to above-mentioned number, and sequence is referring to attached offer
SEQ ID NO:1 shown in, gained sequence is compared in the website NCBI, through compare analysis find, the 16S of bacterial strain JX-1
RRNA gene order and Flavobacterium hibernum DSM 12611THomology highest, be 98.6%, with
Flavobacteriumpectinovorum DSM 6368THomology be 98%, bacterial strain JX-1 and Flavobacterium
hibernum DSM 12611TWith Flavobacterium pectinovorum DSM 6368TAffiliation it is nearest.It utilizes
5.0 software of MEGA by Neighbor-Joining method carry out clustering, as a result through compare analysis find, bacterial strain JX-1 with
Flavobacteriumpectinovorum DSM 6368TBacterium source is 100% in the confidence level of same branch, shows this
Strain is high as the supporting rate of novel bacterial, and fabulous stability is embodied in chadogram, by being somebody's turn to do known to system fungus identification
Bacterial strain JX-1 determines that it is Flavobacterium (Flavobacterium sp.) novel species, has the characteristics that novel bacterial typicalness, from classification
It learns angle and is temporarily named as Flavobacterium (Flavobacterium sp.) JX-1.
The bacterium was preserved in budapest treaty microorganism International Depository Authority: Guangdong Province microorganism fungus kind before the applying date
Collection (GDMCC).Address: 5 building, the building of compound the 59th of GuangZhou, China city martyr Road 100, Guangdong Microbes Inst,
Postcode: 510075.Preservation date on July 30th, 2018, culture presevation number are GDMCC No.60424.It is temporary through microbiology identification
It is named as Flavobacterium (Flavobacterium sp.) JX-1.
For bacterial strain Flavobacterium (Flavobacterium sp.) JX-1 on R2A culture medium, bacterium colony is in yellow, round or do not advise
Then shape, shows smooth translucent, and edge is irregular；Gram's staining is feminine gender, and thallus is in the shape of a rod, and thallus length is about 0.9-
1.2 μm, width is about 0.3-0.5 μm, is not moved, and no whole body cilium, no pod membrane does not form gemma.
Further, Flavobacterium (Flavobacterium sp.) JX-1GDMCC No.60424 provided by the invention has ammonia
Change ability.
Further, Flavobacterium (Flavobacterium sp.) JX-1GDMCC No.60424 provided by the invention is degrading
The application of organic nitrogen.
Further, Flavobacterium (Flavobacterium sp.) JX-1GDMCC No.60424 provided by the invention has drop
The ability of organic nitrogen is solved, therefore is had to amonifying bacteria using and by its organic nitrogen of degrading come the research of purifying water body and utilization
Important value.
Above-mentioned specific technical solution is provided by implementing the present invention, implements the content of present invention, can achieve following beneficial to effect
Fruit:
(1) Flavobacterium (Flavobacterium sp.) JX-1GDMCC No.60424 provided by the invention is a kind of typical case
Novel bacterial can be grown on R2A culture medium, bacterium colony in golden yellow, transparent, surface is smooth, and thallus length is about 0.9-1.2 μm,
Width is about 0.3-0.5 μm；Bacterial strain JX-1 can be grown at 10-37 DEG C, and optimum growth temperature is 28 DEG C, pH value 5.0-11.0 range
Interior to grow, optimal pH 7.0 can be grown under the conditions of NaCl concentration 0-2.0%, and most suitable NaCl concentration is 1%, incubation time
For 24-48h, culture medium is R2A culture medium, purifying water body characteristic with higher, while having that condition of culture is simple, and breeding is fast
The characteristics of, primarily determine that it, for Flavobacterium (Flavobacterium sp.) novel species, has the characteristics that novel bacterial typicalness, from
Taxology angle is temporarily named as Flavobacterium (Flavobacterium sp.) JX-1.
(2) present invention in Poyang Lake dry season water body by separating one plant of Flavobacterium (Flavobacterium
Sp.) JX-1GDMCC No.60424 has certain ammonification ability, can be degraded organic nitrogen using the strain isolated, work as pH value
Under conditions of for 5, cultivation temperature be 25 DEG C, inoculum concentration is 3%, JX-1 bacterial strain has ammonification ability, and organic nitrogen removal rate is higher.
Nitrogenous effluent is purified using the bacterial strain ammonification ability, microorganism fungus kind applied technical field is had a wide range of applications
Value.
Detailed description of the invention
Fig. 1 show Flavobacterium (Flavobacterium sp.) JX-1 systematic growth tree graph.
Fig. 2 show Flavobacterium (Flavobacterium sp.) JX-1 thalli morphology figure.
Fig. 3 show pH value to the influence diagram of Flavobacterium (Flavobacterium sp.) JX-1 ammonification performance.
Fig. 4 show temperature to the influence diagram of Flavobacterium (Flavobacterium sp.) JX-1 ammonification performance.
Fig. 5 show inoculum concentration to the influence diagram of Flavobacterium (Flavobacterium sp.) JX-1 ammonification performance.
Specific embodiment
In the following, illustrating the present invention for embodiment, still, the present invention is not limited to following embodiments.It is selected in the present invention
All raw and auxiliary materials, and the Spawn incubation method selected all is well known in the art selection, the % being related in the present invention
All be weight percentage, unless otherwise indicated except.
The sample that the present invention uses: Poyang Lake dry season water body.
Following basal medium is used in the embodiment of the present invention: R2A culture medium includes yeast extract powder 0.5g, peptone
0.5g, casein hydrolysate 0.5g, glucose 0.5g, soluble starch 0.5g, potassium dihydrogen phosphate 0.3g, anhydrous magnesium sulfate
0.024g, Sodium Pyruvate 0.3g, agar 15.0g add distilled water to 1.0L, pH value 7.2；Ammonification ability test media includes
Peptone 5g, sodium chloride 0.25g, green vitriol 0.01g, dipotassium hydrogen phosphate 0.5g, bitter salt 0.5g add steaming
Distilled water is to 1.0L, pH value 7.2.
Following kit: DNA extraction agent box (Sheng Gong bioengineering limited liability company) is used in the embodiment of the present invention.
Embodiment one: separation, the screening and identification of Flavobacterium JX-1
(1) it separates, purify:
From Poyang Lake dry season water body example, take 10ml water sample be added in 90ml sterile water be uniformly mixed, then into
10 times of gradient dilutions of row obtain dilution, in the test tube for taking the 1ml dilution access culture medium of amonifying bacteria containing 5ml, are placed in 28 DEG C
After cultivating 10-14d, 250ul culture solution is taken out, is reacted with 100ul nessler reagent, shows there is ammonia if sepia is presented
In the presence of for the positive；Draw the culture solution in the amonifying bacteria culture medium test tube being positive, gradient dilution to 10-4, take 100 μ
L dilution is uniformly coated on amonifying bacteria culture medium flat plate, and 28 DEG C are cultivated 2-3 days, repeats 3-4 purifying Flavobacterium JX-1.
Flavobacterium (Flavobacterium sp.) JX-1 after purification is inoculated in the inclined-plane R2A, 28 DEG C incubator constant temperature incubation 2-3 days
Afterwards, it saves backup for 4 DEG C.
(2) classify, identify:
1, Flavobacterium (Flavobacterium sp.) JX-116S rDNA sequence and analysis:
(1) extraction of pcr template DNA
Flavobacterium (Flavobacterium sp.) JX-1 is inoculated in R2A fluid nutrient medium, 28 DEG C of culture 18h are obtained
Somatic cells are obtained, genome DNA is extracted using paramagnetic particle method bacterial genomes DNA extraction agent box.
(2) PCR amplification
Primer:
PrimerA:5'-AGAGTTTGATCCTGGCTCAG-3'；
PrimerB:5’-TACGGCTACCTTGTTACGACTT-3’。
25 μ L of PCR reaction system total volume, PCR amplification condition are 94 DEG C of 5min；94 DEG C of 45s, 56 DEG C of 45s, 72 DEG C of 45s,
30 circulations；72℃10min.
(3) sequencing
Pcr amplification product is sequenced through electrophoresis detection and after purification, and sequence is as shown in SEQ ID NO:1.Gained sequence exists
The website NCBI is compared, through compare analysis find, the 16S rRNA gene order of bacterial strain JX-1 with
Flavobacterium hibernum DSM 12611THomology highest, be 98.6%, with Flavobacterium
pectinovorum DSM 6368THomology be 98%, bacterial strain JX-1 and Flavobacterium hibernum DSM
12611TWith Flavobacterium pectinovorum DSM 6368TAffiliation it is nearest.It is logical using 5.0 software of MEGA
Cross Neighbor-Joining method carry out clustering, as a result through compare analysis find, bacterial strain JX-1 with
Flavobacteriumpectinovorum DSM 6368TBacterium source is 100% in the confidence level of same branch, shows this
Strain is high as the supporting rate of novel bacterial, and fabulous stability is embodied in chadogram, by being somebody's turn to do known to system fungus identification
Bacterial strain JX-1 determines that it is Flavobacterium (Flavobacterium sp.) novel species, has the characteristics that novel bacterial typicalness, from classification
It learns angle and is temporarily named as Flavobacterium (Flavobacterium sp.) JX-1.
2, physiological and biochemical test
By Flavobacterium JX-1 and the highest flavobacterium strain of its homology (F.hibernum DSM12611T、
F.pectinovorum DSM 6368T) Microbiological Characteristics analysis is carried out, concrete outcome is shown in and table 1.
Table 1: compared with flavobacterium strain bio-chemical characteristics result Flavobacterium JX-1 high with its homology
Note :+, it is positive；, negative；W, weakly positive.
As can be seen from Table 1, Flavobacterium of the invention and the highest bacterial strain F.hibernum DSM 12611 of its homologyT
With F.pectinovorum DSM 6368TIt is all had differences in terms of physiological and biochemical property, G+C.Bacterium of the invention
Strain JX-1 has tryptic activity, and nitrate reduction is feminine gender, does not utilize D-arabinose, L-arabinose, D- xylose, D-
Galactolipin, D-Fructose, L- rhamnose, arbutin, D- lactose, D- sucrose, inulin, D- gossypose.With its homology highest two
Strain bacterium F.hibernum DSM 12611TWith F.pectinovorum DSM 6368TPhysiological and biochemical property, G+C mol% with
And cell wall fatty acid species and content etc. all have differences.F.hibernum DSM 12611TWith
F.pectinovorum DSM 6368TAll do not have tryptic activity, nitrate reduction be the positive, can using D- I
Uncle sugar, L-arabinose, D- xylose, D- galactolipin, D-Fructose, L- rhamnose, arbutin, D- lactose, D- sucrose, inulin, D-
Gossypose.In summary data can prove bacterial strain JX-1 and F.hibernum DSM 12611TAnd F.pectinovorum
DSM 6368TIt is not same kind.
3, Analysis of Fatty Acids Composition
Bacterial strain is cultivated on R2A agar at 25 DEG C, and uses the cell of exponential phase of growth.With reference to Miller's (1982)
Method extracts the fatty acid of bacterial strain JX-1 and its reference strains, is measured using gas-chromatography to fatty acid, and the data obtained is logical
It crosses MIDI data library (Hewlett-Packard Co., Palo Alto, California, USA) to be analyzed, finally obtains bacterium
The fatty acid composition and content of strain, as shown in table 2.The content of each ingredient of the fatty acid of bacterial strain JX-1 and reference strains has obviously not
Together.
Table 2: the cell wall fatty acid species and comparision contents of Flavobacterium and its reference strains
Note: TR, it is micro.
Comprehensive 16S rDNA sequence analysis, Phylogenetic Analysis, Microbiological Characteristics analysis, Analysis of Fatty Acids Composition knot
Fruit shows bacterial strain JX-1 really and is one plant of bacterium novel species of Flavobacterium, it is temporarily named as to (Flavobacterium sp.) JX-
1.The bacterium is preserved in Guangdong Province's Culture Collection on July 30th, 2018, address: Xianlie Middle Road, Guangzhou City 100
5 building, the building of compound the 59th, Guangdong Microbes Inst, culture presevation number are GDMCC No.60424.
Form of Flavobacterium (Flavobacterium sp.) the JX-1GDMCC No.60424 under Electronic Speculum is shown in attached drawing 2, should
Bacterium cultivated on plate 36 hours after bacterium colony in yellow, round or irregular shape shows smooth translucent, and edge is irregular；
Gram's staining is feminine gender, and thallus is in the shape of a rod, and thallus length is about 0.9-1.2 μm, and width is about 0.3-0.5 μm, does not move, nothing
Whole body cilium, no pod membrane, does not form gemma.
Embodiment two: Flavobacterium (Flavobacterium sp.) JX-1 ammonification performance measurement
After Flavobacterium (Flavobacterium sp.) JX-1 bacterial strain cultivates 18h in R2A culture medium, it is inoculated into respectively
In 100mL ammonification ability test media, under the conditions of different tests, after 150r/min constant-temperature table culture 40h, culture solution warp
6000r/min centrifuging and taking supernatant, using NH in Nessler's reagent photometer measurement supernatant4 +- N concentration.Investigation pH value (5.0,
6.0,7.0,8.0 and 9.0), cultivation temperature (25 DEG C, 30 DEG C, 35 DEG C and 40 DEG C) and inoculum concentration (1%, 3%, 5%, 7% and
9%) to the influence of bacterial strain ammonification performance, 3 parallel groups are arranged in every group of test, not to be inoculated with the culture medium of amonifying bacteria as empty
White control group.In addition to different temperatures experiment, remaining equal 25 DEG C of culture.
(1) influence of the pH value to bacterial strain ammonification performance
As shown in Fig. 3, the experimental results showed that, pH value is significant to the ammonification capacity of Flavobacterium JX-1 bacterial strain, works as pH
Ammonia nitrogen concentration reaches 43.58mg/L in culture solution when value is 5, as ammonia nitrogen concentration is gradually reduced in the raising culture solution of pH value,
It follows that remove organic nitrogen effect in the relatively low environment of pH value preferable for Flavobacterium JX-1 bacterial strain.
(2) influence of the temperature to bacterial strain ammonification performance
In temperature to the ammonification capacity test result of Flavobacterium JX-1 bacterial strain, as shown in Fig. 4, temperature is to Flavobacterium
The ammoniation of JX-1 bacterial strain influences obviously, there is preferable removal organic nitrogen ability within the scope of 25-30 DEG C, when temperature is lower than 35
The ammonification ability of DEG C Flavobacterium JX-1 bacterial strain is lower.
(3) influence of the inoculum concentration to bacterial strain ammonification performance
In inoculum concentration to the ammonification capacity test result of Flavobacterium JX-1 bacterial strain, as shown in Fig. 5, inoculum concentration is to Huang
The ammoniation of bacillus JX-1 bacterial strain influences obviously, is to have preferable removal organic nitrogen ability within the scope of 1%-3% in inoculum concentration,
Ammonia nitrogen concentration is not less than 43.78mg/L in culture solution, and removal organic nitrogen effect is preferable, the Flavobacterium JX- when inoculum concentration is greater than 5%
The ammonification ability of 1 bacterial strain is declined, but inoculum concentration in 1%-9% range culture solution ammonia nitrogen concentration in 44.0-40.0mg/L
Between, removal organic nitrogen ability is stronger.
Experiments have shown that, the present invention in Poyang Lake dry season water body by separating one plant of Flavobacterium above
(Flavobacterium sp.) JX-1GDMCC No.60424 has certain removal organic nitrogen ability, utilizes what is isolated
Strain can degrade organic nitrogen, and when pH value is 5, cultivation temperature is 25 DEG C, under conditions of inoculum concentration is 3%, JX-1 bacterial strain has
Ammonification ability, organic nitrogen removal rate are higher.
Embodiment three: Flavobacterium (Flavobacterium sp.) JX-1 ammonification performance measurement in sewage
By Flavobacterium (Flavobacterium sp.) JX-1 bacterial strain in R2A culture medium after enrichment culture 18h, with 3%
It is inoculated into 100mL polluted lake water, under conditions of pH value is 5, after 25 DEG C of culture 40h of 150r/min constant-temperature table, sewage warp
6000r/min centrifuging and taking supernatant, using NH in Nessler's reagent photometer measurement supernatant4 +- N concentration.Measure ammonia nitrogen in sewage
Concentration reaches 41.69mg/L, it is seen that Flavobacterium JX-1 bacterial strain has ammonification ability, and organic nitrogen removal rate is higher.Utilize the bacterial strain
Degradation organic nitrogen ability purifies nitrogenous effluent, has a wide range of applications valence for microorganism fungus kind applied technical field
Value.
The above embodiment is merely an example for clearly illustrating the present invention, and does not limit the embodiments.
For those of ordinary skill in the art, other various forms of variations can also be made on the basis of the above description
Or it changes.There is no necessity and possibility to exhaust all the enbodiments.And the obvious variation thus extended
Or it changes and is still in the protection scope of this invention.
Sequence table
<110>institute of microbiology, Jiangxi Prov. Academy of Science
Li Ya
<120>a kind of Flavobacterium JX-1 and its application in waste water control
<141> 2018-12-14
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1436
<212> DNA
<213>Flavobacterium (Flavobacterium sp.)
<400> 1
gatgaacgct agcggcaggc ttaacacatg caagtcgagg ggtatatgtc ttcggatata 60
gagaccggcg cacgggtgcg taacgcgtat gcaatctacc ttttacagag ggatagccca 120
gagaaatttg gattaatacc tcatagcata gcagcttcgc atgaagccac tattaaagtc 180
acaacggtaa aagatgagca tgcgtcccat tagctagttg gtaaggtaac ggcttaccaa 240
ggctacgatg ggtaggggtc ctgagaggga gatcccccac actggtactg agacacggac 300
cagactccta cgggaggcag cagtgaggaa tattggacaa tgggcgcaag cctgatccag 360
ccatgccgcg tgcaggatga cggtcctatg gattgtaaac tgcttttgta cgagaagaaa 420
cactacttcg tgaagtagct tgacggtatc gtaagaataa ggatcggcta actccgtgcc 480
agcagccgcg gtaatacgga ggatccaagc gttatccgga atcattgggt ttaaagggtc 540
cgtaggcggt ttagtaagtc agtggtgaaa gcccatcgct caacggtgga acggccattg 600
atactgctag acttgaatta ttaggaagta actagaatat gtagtgtagc ggtgaaatgc 660
ttagagatta catggaatac caattgcgaa ggcaggttac tactaatgga ttgacgctga 720
tggacgaaag cgtgggtagc gaacaggatt agataccctg gtagtccacg ccgtaaacga 780
tggatactag ctgttgggag caatttcagt ggctaagcgg aagtgataag tatcccacct 840
ggggagtacg ttcgcaagaa tgaaactcaa aggaattgac gggggcccgc acaagcggtg 900
gagcatgtgg tttaattcga tgatacgcga ggaaccttac caaggcttaa atgtagattg 960
accgatttgg aaacagatct ttcgcaagac aatttacaag gtgctgcatg gttgtcgtca 1020
gctcgtgccg tgaggtgtca ggttaagtcc tataacgagc gcaacccctg ttgttagttg 1080
ccatcgagtg atgtcgggaa ctctaacaag actgccagtg caaactgtga ggaaggtggg 1140
gatgacgtca aatcatcacg gcccttacgc cttgggctac acacgtgcta caatggccgg 1200
tacagagagc agccactggg tgaccaggag cgaatctata aagccggtca cagttcggat 1260
cggagtctgc aactcgactc cgtgaagctg gaatcgctag taatcggata tcagccatga 1320
tccggtgaat acgttcccgg gccttgtaca caccgcccgt caagccatgg aagctggggg 1380
tgcctgaagt tggtgaccgc aaggagctgc ctagggtaaa actggtaact agggct 1436
Claims (4)
1. a kind of Flavobacterium (Flavobacterium sp.) JX-1, which is characterized in that the Flavobacterium
(Flavobacterium sp.) JX-1 culture presevation number be GDMCC No.60424.
2. a kind of as described in claim 1 Flavobacterium (Flavobacterium sp.) application of the JX-1 in sewage treatment.
3. a kind of as claimed in claim 2 Flavobacterium (Flavobacterium sp.) JX-1 is in answering in sewage treatment
With, which is characterized in that after Flavobacterium JX-1 bacterial strain cultivates 18h in R2A culture medium, by weight percentage, take 1%-3% Huang bar
Bacterium JX-1 is inoculated into 100mL ammonification ability test media, when 25 DEG C of temperature, pH value 5.0-7.0,150 r/min constant temperature
Shaking table culture 40h.
4. a kind of as claimed in claim 2 Flavobacterium (Flavobacterium sp.) JX-1 is in answering in sewage treatment
With, which is characterized in that Flavobacterium (Flavobacterium sp.) JX-1 bacterial strain after enrichment culture 18h, presses in R2A culture medium
Weight percent meter takes 1%-3% Flavobacterium JX-1 to be inoculated into 100mL polluted lake water, under conditions of pH value is 5,150r/
25 DEG C of culture 40h of min constant-temperature table.
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| CN113429022A (en) * | 2021-07-01 | 2021-09-24 | 江西省科学院微生物研究所 | Modular rapid culture biogas slurry wastewater treatment system and operation method thereof |
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| CN105712491A (en) * | 2016-03-04 | 2016-06-29 | 中国科学院生态环境研究中心 | Denitrification device |
| CN107574117A (en) * | 2017-07-04 | 2018-01-12 | 河北地质大学 | A kind of preparation facilities and its method of penicillin resistant class amonifying bacteria microbial inoculum |
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| CN105712491A (en) * | 2016-03-04 | 2016-06-29 | 中国科学院生态环境研究中心 | Denitrification device |
| CN107574117A (en) * | 2017-07-04 | 2018-01-12 | 河北地质大学 | A kind of preparation facilities and its method of penicillin resistant class amonifying bacteria microbial inoculum |
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| 丁梦娇等: "植烟土壤中微生物特性及氨化_亚硝化菌分离鉴定与活性研究", 《中国生态农业学报》 * |
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| CN113429022A (en) * | 2021-07-01 | 2021-09-24 | 江西省科学院微生物研究所 | Modular rapid culture biogas slurry wastewater treatment system and operation method thereof |
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