CN104560775A - Enterobacter cloacae SRPG-70 and application thereof in salt stress relieving and growth promoting - Google Patents
Enterobacter cloacae SRPG-70 and application thereof in salt stress relieving and growth promoting Download PDFInfo
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- CN104560775A CN104560775A CN201410386813.9A CN201410386813A CN104560775A CN 104560775 A CN104560775 A CN 104560775A CN 201410386813 A CN201410386813 A CN 201410386813A CN 104560775 A CN104560775 A CN 104560775A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
Abstract
The invention discloses enterobacter cloacae SRPG-70 which is screened from Sinkiang salinity soil and has the effect of remarkably relieving salt stress to promote crop growth. Authentications in the aspects of physiological and biochemical characteristics show that the enterobacter cloacae SRPG-70 is a gram negative bacterium. By utilizing the characteristics of the enterobacter cloacae SRPG-70, pot experiment proves that the germination rate in treatment of 0.8% of NaCl is improved by 22.4% relative to sterile treatment; under salinization condition, the dry weight of cotton is improved by 28.8% by the SRPG-70; the plant height is increased by 14.2%; under salinization-free condition, the dry weight of cotton is improved by 14.9% by the SRPG-70; the plant height is improved by 11.5%. Through electron microscopy observation, the SRPG-70 can be successfully colonized at the middle parts of root tips and root base parts of plant roots. The cotton inoculated with the SRPG-70 is capable of adjusting the uptake, accumulation, transportation and distribution of metal ions. The SRPG-70 can be used for replacing chemical fertilizers to provide salt stress relieving and growth promoting effects and has the advantages of being strong in adaptive capacity to environment and stable in application effect; therefore, the SRPG-70 can be used for developing microbial agents and has wide application prospect to solve the soil salinization problem.
Description
Technical field
The present invention relates to a kind of enterobacter cloacae (Enterobacter cloacae) SRPG-70, belong to agricultural biological technical field.More specifically, the present invention relates to a strain can the enterobacter cloacae SRPG-70 of molten phosphorus potassium decomposing fixed nitrogen and Salte stress-relieving and growth-promoting purposes thereof.
This bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on June 30th, 2014, preserving number is CGMCC No.9395, preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, and this bacterial strain can replace chemical fertilizer Promoting plant growth.More particularly, this bacterial strain can improve the germination rate of cottonseed under condition of salt stress, improves the growth of cotton seedling biomass.
Background technology
The arid and semi-arid weather conditions of the Northwest's uniqueness make water resource critical shortage.In recent years, the application of under-film drip irrigation pattern fundamentally changes mode of agriculture, greatly facilitates the development of agricultural production.But drip irrigation technique is applied rapidly and also brought new problem, the secondary salinization of soil is namely caused to occur.In recent years, various types of Secondary Saline is day by day serious, causes crop to be short of seedling, break ridge, production declining, seriously constrains agriculture in arid areas production development.But geography, the climate characteristic of its uniqueness simultaneously, also breed unique and abundant microbial resources, improve the huge applications potentiality of crop tolerance to salt resistance based on rhizosphere growth-promoting bacterium (PGPR), researcher both domestic and external is paid close attention to one after another.
Research in recent years shows, beneficial rhizosphere microorganisms can promote the growing of crop, improve output, and crop more can be helped to tolerate some adverse environmental factors.Therefore, utilize mycorrhizal fungi and PGPR exploitation that crop can be helped to improve tolerance to the adverse circumstance such as saline and alkaline, and the microorganism formulation with good growth-promoting functions receive publicity day by day.Rhizosphere beneficial microbe is ubiquitous in soil, can with the root growth mutualistic symbiosis body of plant, after plant growth-promoting rhizobacteria and plant form mycorhiza, plant is that the growth of bacterial strain provides carbon source and energy, and rhizosphere bacterium then improves the nutrition condition of plant by its metabolite, comprise the absorption of nutrient and moisture, particularly can improve plant absorption phosphorus, the ability of fixed nitrogen, promotes the absorption of Plant To Nutrient, thus the biomass increasing plant carrys out Promoting plant growth.Much research finds, under salt stress, mycorhiza strengthens the absorption of Plant To Nutrient material, suppresses excessive Na salt in the accumulation of above-ground plant parts, promotes the growth of plant.Especially, under the condition of P shortage, the improvement of P element nutrition is the key that plant anti-salt ability increases.After inoculation, above-ground plant parts P content is apparently higher than contrast, and under ground portion Na content is higher, decreases coercing plant corpus.Saline Alkali Stress, on the impact of plant growth, mainly causes plant physiology lack of water.Improve plant water absorbing capacity or improve water use efficiency, indirectly can increase the salinity and alkalinity resistance of plant.Mycorhiza by changing its water absorbing capacity of organization structure of the plant, and then increases its saline-alkali tolerance.Under salt stress, mycorrhizal fungi, by changing carbohydrate and amino acid whose content and composition in plant corpus, changes the osmotic equilibrium in root tissue, reduces the absorption of plant to Na ion and Cl ion, improves plant salt tolerance ability.Postgraduate's fat azospirillum (Azospirillum lipoferum) ngfp15 such as Bacilio M surely can grow at the wheat rhizosphere of non-earth culture under NaCl coerces, and promote the growth of wheat and the accumulation of biomass, alleviate injury (the Bacilio M that salt stress causes wheat, Rodriguez H, Moreno M, Hernandez JP.Mitigation of salt stress in wheat seedling by a gfp-tagged Azospirillum lipoferum [J] .Biology and Fertility of Soils, 40:2004, 188-193).Dilfuza E etc. are from the cotton of the arid desert of Uzbekistan, wheat, tomato, melon is separated with the root system of alfalfa and obtains Pseudomonas alcaligenes (Pseudomonas alcaligenes) PsA15, bacillus polymyxa (Bacillus polymyxa) BcP26 and mycobacterium (Mycobacterium phlei) MbP18, these bacterial strains can significantly improve the biomass of cotton and the pea grown in lean soil, make the N in plant body, P, K element content is significantly improved, and these bacterial strains can be secreted plant hormone and have nitrogenase activity (Dilfuza.E, Gisela H.Effect of plant growth-promoting bacteria on growth and nutrient uptake of cotton and pea in a semi-arid region of Uzbekistan [J] .Journal of Arid Environments, 2004, 56:293-301), the people such as the Mayaka S of Israel's Hebrew University isolate the rhizospheric microorganism that 7 strains have 1-amino-cyclopropane-carboxylic acid (ACC) deaminase active in the arid salt affected soil in Arava area, Israel south, DNA analysis is shown to be achromobacteria.These microorganisms can strengthen the ability that tomato salt-tolerant is coerced, significantly improve tomato seedling amount of growth (Mayaka S of (172mM NaCl) in high salt concentration environment, Tirosh T, Glick BR.Plant growth-promoting bacteria that confer resistance to water stress in tomatoes and peppers [J] .Plant Science 2004,166:525-530).
But about the geography of Xinjiang uniqueness, the bacterial strain of climate characteristic seldom have report, in addition relevant enterobacter cloacae can alleviate salt stress and Promoting plant growth have no report.Independently screen enterobacter cloacae strain by the present inventor and can improve the germination rate of cottonseed under condition of salt stress, and promote the growth of cotton in seedling stage, in actual application, available bacterium liquid, to crop seed seed soaking or after planting in conjunction with drip irrigation application, reaches the object of Salte stress-relieving and growth-promoting.
Summary of the invention
Each mainly planting the typical case's Secondary Saline field of cotton region gathers Cotton rhizosphere soil sample, through the strain Gram-negative bacteria that separation and purification obtains from North SinKiang to the object of this invention is to provide one.
One strain can be alleviated salt stress and promote that the preserving number of the bacterial strain enterobacter cloacae SRPG-70 of cotton growth is CGMCC No.9395 under being used for condition of salt stress.
Enterobacter cloacae SRPG-70 strain isolation of the present invention grows good cotton rhizosphere in the cotton field that salination is serious, primary dcreening operation medium DF and ADF medium is utilized to be enriched to the bacterial strain with acc deaminase activity, be the Preliminary screening that planting matrix does acc deaminase active bacterial strain again with perlite, the screening again finally doing acc deaminase active bacterial strain with natural saline soil for planting matrix obtains the SRPG-4 producing acc deaminase activity, identify through morphology, Physiology and biochemistry and 16Sr DNA, this bacterial strain meets enterobacter cloacae feature.Identify thus, bacterial strain enterobacter cloacae (Enterobacter cloacae).
The finding that there is of new strains is beneficial to promotion and utilizes PGPR to develop to be applicable to drip irrigation cultivation mode, and middle low secondary salinization is ploughed the biological salt-decomposition agent research and development used.
Enterobacter cloacae SRPG-70 of the present invention surely can grow at plant roots base portion under salt stress, thus promotes cotton cottonseed to germinate and the growth of cotton seedling biomass, reduces the use of chemical fertilizer, simultaneously free from environmental pollution.
Between Cotton Fields of the present invention, using method is specific as follows:
Appropriate crop seed is soaked in bacteria suspension inoculation 4 hours, or after planting apply in conjunction with drip irrigation.
Compared with prior art, the present invention has the following advantages:
Enterobacter cloacae SRPG-70 of the present invention derives from the salinization soil of Xinjiang Special Geographic weather conditions, the cotton of inoculation SRPG-70 bacterial strain can reduce Na absorptive amount compared with the control, alleviate salt stress simultaneously, SRPG-70 bacterial strain, salt-free and significantly can promote the accumulation of Ca, K, Mg, Fe element in cotton plants root system and leaf tissue under having salt condition, controls the growth of economic crops under salt stress based on cotton well.This bacterial strain can replace the Salte stress-relieving and growth-promoting of chemical fertilizer to crop to have effect well, have adaptive capacity to environment strong, the advantages such as effect is stable, can be used for planting in salinization soil, have good effect to the output increased of crop and the utilization improvement of salinization soil.
Accompanying drawing explanation
Fig. 1 SRPG-70 root determine grow (A: have foundation portion under salt condition surely grow 40000 ×, B: have the tip of a root under salt condition surely grow 10000 ×, C: have surely to grow 10000 under salt condition in the middle part of root ×, D: surely grow 10000 × in the middle part of root under salt-free conditions)
Embodiment
Be described in further detail invention below by specific embodiment, following examples are descriptive, are not determinate, can not limit protection scope of the present invention with this.
Embodiment 1: the qualification of physio-biochemical characteristics
The qualification of physio-biochemical characteristics and the qualification of 16S rDNA molecule are carried out, from the kind of molecular level determination Salte stress-relieving and growth-promoting bacterium to Salte stress-relieving and growth-promoting bacterium SRPG-70.
Qualification result is in table 1.
The physio-biochemical characteristics of table 1 bacterial strain SRPG-70
+: positive;-: negative
16S rDNA identifies
The 16S rDNA sequence fragment size of the SRPG-70 that order-checking obtains is 1459bp, sequence homology compare of analysis is carried out in NCBI website, in conjunction with the qualification result of physiological and biochemical property, this bacterial strain SRPG-70 morphology, physiological and biochemical property meet enterobacter cloacae feature.Identify thus, bacterial strain enterobacter cloacae (Enterobacter cloacae).
Embodiment 2: after inoculating enterobacter cloacae SRPG-70 bacterium under condition of salt stress, bacterial colonization measures
SEM (SEM) (JSM-670 of the Tokyo JEOL company) situation of growing of determining to enterobacter cloacae SRPG-70 is utilized to carry out observation and analysis.The a series of ethanolic solution process of Levant Cotton Root sample, finally with 100% acetone dehydration, again adopts CPD 030 (BAL-TEC, Germany) method, carries out critical point drying with liquid carbon dioxide.Then metal spraying (sputter coating machine SCD, 005, BAL-TEC, Germany) is carried out to sample and carry out SEM inspection again.
Take from rhizome base portion, show with the scanning electron microscopic observation result of the sample of the tip of a root in the middle part of root, SRPG-70 bacterial strain all has bacterium colony to distribute at each root segment, rhizome base portion portion root segment bacterial density is larger, general easilier in the visual field, see a large amount of bacterial clumps (Figure 1A), but bacterial density declines (Fig. 1 C) to some extent in the middle part of root; At the tip of a root, choose the visual field amplify after more rare or often can't see bacterial clump (Figure 1B), cortical cell junction is then easy to find microorganism, and density is quite large.This display bacterium surely grows on the surface of root system and has stronger regioselectivity, is easy to the region of attachment or nutrients many (root secretion is more) more easily for Salte stress-relieving and growth-promoting bacterium grows surely.In addition, under observation also find that SRPG-70 organisms is concentrated, and sticks to each other agglomerating and is attached to cotton root table.
Embodiment 3: inoculate the impact of enterobacter cloacae SRPG-70 on cotton biomass under condition of salt stress
With bacterium process cotton seed, when in saline-alkali soil pot experiment, cotton grows to tri-leaf period, the length of all plant of measuring same process from growing point to the tip of a root and plant height, average, be the average plant height of this process plant.Weigh the gross weight of all plant of same process, then it is placed in drying box, complete at 110 DEG C 10min, then dry 24h to constant weight at proceeding to 80 DEG C, and taking-up is weighed.Gross weight before oven dry, divided by weighing strain number, obtains the mean fresh of this process plant.Gross weight after oven dry, divided by weighing strain number, obtains the average dry weight of this process plant.The growing state of Growth of Potted Cotton is in table 2.
Table 2 SRPG-70 is to the influence of Cotton Seed rate and cotton biomass
Note: CK-NS, aseptic without Ficus caricaL; SRPG-70-NS, SRPG-70 bacterial strain is without Ficus caricaL; CK-S, aseptic 0.8% Ficus caricaL; SRPG-70-S, SRPG-70 bacterial strain 0.8% Ficus caricaL.With marking different lowercase after column data, this represents significant difference (p < 0.05) in 5% level.
0.8%NaCl has very strong coercion to cotton, and the germination rate of salt-free normal control CK-NS is 88.0%, exceeds salt adding contrast CK-S 56.6%.After cotton seed inoculation SRPG-70 bacterial strain, normally do not significantly improve without the germination rate under salt environment and strain rate but under condition of salt stress, show obvious raising effect, SRPG-70 comparatively CK-S germination rate improves 22.4%, and strain rate improves 60.5%.Be studied the impact of SRPG-70 bacterial strain on cotton biomass accumulation, result shows: SRPG-70 bacterial strain has stronger Salte stress-relieving and growth-promoting effect, and under saline and alkaline condition, the dry weight of cotton is improve 28.8% by SRPG-70; 14.2% is improve to the plant height of cotton.Under salt-free conditions, SRPG-70 to be significantly improved effect to the biomass accumulation of cotton seedling.The dry weight of SRPG-70 to cotton improves 14.9%, is respectively 11.5% to the raising of the plant height of cotton.Biomass data under more saline and alkaline condition and salt-free conditions, can find out that SRPG-70 shows more obvious facilitation to cotton growth under saline-alkali environment condition.
Can find out that enterobacter cloacae SRPG-70 bacterial strain to be all significantly improved effect to the germination rate of cotton in salt affected soil, dry weight, fresh weight and plant height by above example.
Embodiment 4: inoculate enterobacter cloacae SRPG-70 absorbs nutritive element impact on cotton seedling under condition of salt stress
To the five kinds of constituent content measurement results of Na, Ca, K, Fe, Mg in cotton plants in shown in table 3,4 (significance that letter representation is different, P < 0.05).Absorbing state below for often kind of element is analyzed.
Table 3 SRPG-70 is on the impact of cotton leaf Na, Ca, K, Fe, Mg constituent content
Table 4 SRPG-70 is on the impact of cotton root system Na, Ca, K, Fe, Mg constituent content
SRPG-70 is on the impact of cotton Na element absorption under salt stress
Under salt-free conditions, SRPG-70 process cotton plant to the absorptive amount of Na a little more than contrast.Root system and the blade Na constituent content of the rear all process cottons of NaCl process all significantly rise (table 3,4), the cotton of inoculation SRPG-70 bacterial strain compared with the control Na absorptive amount obviously reduces, the leaf Na/ root Na ratio of SRPG-70 is 1.11, is starkly lower than the ratio (1.2) of contrast.This illustrates that SRPG-70 shows cotton under reduction salt stress the effect of the absolute absorption amount of Na element.
SRPG-70 is on the impact of cotton Ca element absorption under salt stress
The distribution condition of cotton to Ca element is that blade accumulation is higher than root system.After NaCl process, adjoining tree blade and root system Ca constituent content all obviously decline (table 3,4).Root system fall is greater than blade, is respectively 63.2% and 44.6%, and this illustrates that the shortage of plant root Ca element is one of the most responsive index of salt damage.0.8%NaCl coerces down, the absorption of cotton plants to Ca element of SRPG-70 bacterial strain process presents the rule identical with contrast, unlike, SRPG-70 bacterial strain can promote that cotton is to the absorption of Ca element, salt-free and significantly can promote the accumulation of Ca element in cotton plants root system and leaf tissue under having salt condition, under salt stress, the fall of Ca will lower than contrast.
The impact that SRPG-70 absorbs cotton K element under salt stress
As the most responsive another one index of salt damage, the distribution condition of cotton to K element is that root system accumulation is higher than blade.Adjoining tree blade under salt stress and root system K element content all obviously decline (table 3,4), and the fall difference 36.4% and 39.5% of root system and blade, fall is suitable.SRPG-70 bacterial strain is salt-free and significantly can promote the accumulation of K element in cotton plants root system and leaf tissue under having salt condition.
SRPG-70 is on the impact of cotton Mg element absorption under salt stress
In contrast cotton root system, Mg constituent content raises to some extent, and in blade, Mg constituent content declines (table 3,4).Illustrate that 0.8%NaCl coerces root system Mg
2+inhalation effects is little, but Na
+limit Mg
2+transport to blade by xylem, thus cause blade Mg constituent content to decline.Experiment finds, SRPG-70 can increase the absorption of cotton to Mg element, but does not affect its distribution in cotton plant body and transport.
SRPG-70 is on the impact of cotton Fe element absorption under salt stress
Different from Ca, K element, Fe constituent content increases (table 3,4) to some extent on the contrary after salt stress.From distribution, in root system, Fe constituent content is higher than blade.Under salt stress, the amplification of contrast Fe element content in root system is 5.9%, the amplification 15.7% in blade.SRPG-70 is salt-free and have under salt condition and significantly promote cotton plants to the absorption of Fe element.In addition, cotton after SRPG-70 inoculation presents the situation different from contrast on Fe Elemental partition, namely the allocation proportion of Fe element in blade and root system reduces, and the distribution ratio of contrast Fe element in blade and root system is: 1.32 (salt-free) and 1.21 (salt addings).The numerical value of SRPG-70 process cotton plant is then 1.05 (salt-free) and 1.07 (salt addings), and this illustrates that the transport of SRPG-70 on Fe element is assigned impact.
Claims (8)
1. an Enterobacter cloacae (Enterobacter cloacae) SRPG-70 bacterial strain, it is characterized in that, on June 30th, 2014 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), preserving number is CGMCC No.9395, and preservation name is called enterobacter cloacae Enterobacter cloacae SRPG-70.
2. bacterial strain according to claim 1, it is characterized in that, the growth that described enterobacter cloacae SRPG-70 can be good in DF medium, is creamy white, and circular or subcircular, central uplift, opaque, not chromogenesis, colony diameter is about 1.5mm.
3. bacterial strain according to claim 1, is characterized in that, identify that it is a strain Gram-negative bacteria through physiological and biochemical property, can dissolved organic phosphorus, and the nitrogen in fixed air, dissolves potassium element etc.
4. bacterial strain according to claim 1, it is characterized in that having salt resistance well, it can grow in 8%NaCl.
5. the feature involved by claim 2,3, is characterized in that to improve the germination rate of crop and promote the increase of cotton seedling biomass under the condition having salt stress.
6. bacterial strain according to claim 1, the feature involved by claim 2,3, is characterized in that, this bacterium can in the middle part of the tip of a root of plant and foundation portion surely grow, single bacterium is shaft-like, villous.
7. the application involved by claim 4, described crop is the crops such as cotton, capsicum and processing tomato.
8. the application involved by claim 6, the cotton of inoculation SRPG-70 bacterial strain can reduce Na absorptive amount compared with the control, and SRPG-70 bacterial strain is salt-free and significantly can promote the accumulation of Ca, K element in cotton plants root system and leaf tissue under having salt condition; SRPG-70 can increase the absorption of cotton to Mg element, but does not affect its distribution in cotton plant body and transport, and the transport of SRPG-70 on Fe element is assigned impact.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105255769A (en) * | 2015-11-03 | 2016-01-20 | 湖南中烟工业有限责任公司 | Enterobacter cloacae and application thereof |
| CN105925503A (en) * | 2016-05-17 | 2016-09-07 | 山东省林业科学研究院 | Salt-tolerant rhizosphere growth-promoting enterobacter cloacae and application thereof |
| CN108795797A (en) * | 2018-05-24 | 2018-11-13 | 珠海市现代农业发展中心(珠海市金湾区台湾农民创业园管理委员会、珠海市农渔业科研与推广中心) | Raw enterobacter cloacae and its application in one plant of maize root system |
| CN109536410A (en) * | 2018-12-17 | 2019-03-29 | 石河子大学 | A kind of salt tolerant growth-promoting composite bacteria agent and its preparation method and application |
| CN111657301A (en) * | 2020-05-27 | 2020-09-15 | 西安工程大学 | Wettable plant rhizosphere microorganism growth promoting powder and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1368549A (en) * | 2002-03-06 | 2002-09-11 | 中国科学院武汉病毒研究所 | Tharingiensis bacillus strain with broad-spectrum insecticiding activity and its preparing process |
| CN101531980A (en) * | 2009-04-13 | 2009-09-16 | 四川农业大学 | Bacillus thuringiensis HS18-1 and application thereof |
-
2014
- 2014-08-03 CN CN201410386813.9A patent/CN104560775A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1368549A (en) * | 2002-03-06 | 2002-09-11 | 中国科学院武汉病毒研究所 | Tharingiensis bacillus strain with broad-spectrum insecticiding activity and its preparing process |
| CN101531980A (en) * | 2009-04-13 | 2009-09-16 | 四川农业大学 | Bacillus thuringiensis HS18-1 and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 岳海涛: "具ACC脱氨酶活性解盐促生菌筛选及其对棉花吸收离子的影响", 《中国优秀硕士学位论文全文数据库》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105255769A (en) * | 2015-11-03 | 2016-01-20 | 湖南中烟工业有限责任公司 | Enterobacter cloacae and application thereof |
| CN105925503A (en) * | 2016-05-17 | 2016-09-07 | 山东省林业科学研究院 | Salt-tolerant rhizosphere growth-promoting enterobacter cloacae and application thereof |
| CN105925503B (en) * | 2016-05-17 | 2019-05-28 | 山东省林业科学研究院 | One plant of salt tolerant rhizosphere growth-promoting enterobacter cloacae and its application |
| CN108795797A (en) * | 2018-05-24 | 2018-11-13 | 珠海市现代农业发展中心(珠海市金湾区台湾农民创业园管理委员会、珠海市农渔业科研与推广中心) | Raw enterobacter cloacae and its application in one plant of maize root system |
| CN108795797B (en) * | 2018-05-24 | 2021-09-07 | 珠海市现代农业发展中心(珠海市金湾区台湾农民创业园管理委员会、珠海市农渔业科研与推广中心) | Corn root system endophytic enterobacter cloacae and application thereof |
| CN109536410A (en) * | 2018-12-17 | 2019-03-29 | 石河子大学 | A kind of salt tolerant growth-promoting composite bacteria agent and its preparation method and application |
| CN111657301A (en) * | 2020-05-27 | 2020-09-15 | 西安工程大学 | Wettable plant rhizosphere microorganism growth promoting powder and preparation method thereof |
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Application publication date: 20150429 |