CN105670961A

CN105670961A - Inorganic phosphorus solubilizing plant growth promoting rhizobacteria strain NG-33 and application thereof

Info

Publication number: CN105670961A
Application number: CN201610043740.2A
Authority: CN
Inventors: 樊宪伟; 陈鑫鑫; 李有志
Original assignee: Guangxi University
Current assignee: Guangxi University
Priority date: 2016-01-22
Filing date: 2016-01-22
Publication date: 2016-06-15
Anticipated expiration: 2036-01-22
Also published as: CN105670961B

Abstract

The invention belongs to the technical field of plant growth promoting rhizobacteria and particularly relates to inorganic phosphorus solubilizing plant growth promoting rhizobacteria strain NG-33 and application thereof.The inorganic phosphorus solubilizing plant growth promoting rhizobacteria strain NG-33 is taxonomically named as Enterobacter cloacae NG-33 and preserved in the China Center for Type Culture Collection, and a preservation number of the plant growth promoting rhizobacteria strain NG-33 is CCTCC M 2016056.The plant growth promoting rhizobacteria strain NG-33 is obtained for the first time by screening and separating from soil of the Longgang National Natural Reserve in the Guangxi Zhuang Autonomous Region and is capable of realizing high-yield gluconic acid and acetic acid, dissolving hardly-soluble inorganic phosphorus to increase soluble phosphorus content in soil, relieving phosphorus deficiency of plants and improving soil conditions, and accordingly plant growth is promoted.

Description

A kind of plant growth-promoting bacterial strain NG-33 and application thereof separating inorganic phosphorus

Technical field

The invention belongs to plant growth-promoting bacteria technical field, what be specifically related to is a kind of plant growth-promoting bacterial strain NG-33 and application thereof of separating inorganic phosphorus.

Background technology

Chemical fertilizer plays great function in modern agricultural production. But the increase along with fertilizer application amount, its utilization ratio reduces year by year. The residual of agricultural chemicals and chemical fertilizer, a large amount of objectionable impurities to Environment release, pollutes soil, water source and food, human health and living environment is constituted very big threat, it is desired to preserve the ecological environment and the cry of production safety food day by day strong. Therefore, develop the new source of manure substituting chemical fertilizer, with adapt to development green agriculture and green food to need be the task of top priority. And microbial fertilizer can make invalid nutrition effectuation in soil, prevention and corntrol corps diseases, reduce the use of agricultural chemicals and chemical fertilizer, be solve soil, water source and food contamination basic by way of, be generally considered the method for the raising crop yield of a kind of environmental friendliness, economical and effective.

Microbial fertilizer is the particular product that a class contains living microorganisms, is applied in agriculture production, it is possible to obtain specific fertilizer effect. Wherein in goods, live microorganism plays keying action. At present, generally microorganism fertilizer material products is divided into two big classes: a class is the microbial fertilizer of narrow sense, refer to the vital movement by microorganism, add the supply of plant nutrient, comprise the total supply of plant nutrient in soil and production environment, cause the improvement of plant nutrition situation, and then increase yield. The representative of this quasi-microorganism fertilizer is rhizobia fertilizer; Another class is the microbial fertilizer of broad sense, refer to the vital movement by wherein microorganism, not only can improve the supply of plant nutrient, plant growth hormones can also be produced, promote that plant is to the pathogenic effects absorbing or having some pathogenic micro-organism anti-short of money of nutritive element, alleviates diseases and pests of agronomic crop brief introduction and improves crop yield. Compared with chemical fertilizer, microbial fertilizer has the following advantages: not spoiled soil structure; Protecting ecology, free from environmental pollution, people and animals are nontoxic; Fertilizer efficiency is lasting; Improve crop yield, improve crop quality; With low cost, economical and effective.

Plant growth-promoting rhizobacteria (Plantgrowthpromotingrhizobacteria, PGPR) it is that a class energy high-density grows the microorganism at plant rhizosphere surely, have Suppressing phytopathogens, rhizosphere harmful microorganism concurrently, and Promoting plant growth and increase the effect of crop yield. As the valuable source storehouse of bio-feritlizer and biological pesticide, the investigation and application of PGPR plays the important and pivotal role. Research and develop microbial fertilizer from the angle of resource regeneration and then comprehensive utilization of resources and environment protection are had more realistic meaning.

The information being disclosed in this background section only is intended to increase the understanding of the general background to the present invention, and should not be regarded as admitting or imply that this information structure has been prior art that persons skilled in the art are known in any form.

Summary of the invention

The problem that the present invention solves is to provide a kind of bacterial strain NG-33 and the application thereof that can separate inorganic phosphorus, and this bacterium is a kind of new plant growth-promoting rhizobacteria, can be applicable to the preparation of plant growth-promoting agent or microbial fertilizer.

The object of the present invention is achieved through the following technical solutions:

The present invention discloses a kind of plant growth-promoting bacterial strain NG-33 separating inorganic phosphorus, its taxonomy called after enterobacter cloacae NG-33 (Enterobactercloacae), being preserved in China typical culture collection center on January 21st, 2016, preserving number is CCTCCM2016056.

Present invention also offers the plant growth-promoting bacterial strain NG-33 of described solution inorganic phosphorus in the purposes increased in soil in solvable phosphorus content.

Present invention also offers the purposes of the plant growth-promoting bacterial strain NG-33 of described solution inorganic phosphorus in high yield gluconic acid, acetic acid.

Present invention also offers the purposes of the plant growth-promoting bacterial strain NG-33 of described solution inorganic phosphorus in synthetically grown element IAA, it is characterised in that, described plant growth-promoting bacterial strain NG-33 can utilize tryptophane synthetically grown element IAA.

Present invention also offers the purposes of the plant growth-promoting bacterial strain NG-33 of described solution inorganic phosphorus in Promoting plant growth.

Present invention also offers the plant growth-promoting bacterial strain NG-33 of described solution inorganic phosphorus in the purposes improved in content of available phosphorus in soil.

Present invention also offers the plant growth-promoting bacterial strain NG-33 of described solution inorganic phosphorus in the purposes prepared in Promoting plant growth preparation.

As preferably, described Promoting plant growth preparation is microbiobacterial agent or microbial fertilizer.

Compared with prior art, the present invention has following useful technique effect:

1. enterobacter cloacae NG-33 provided by the invention is that from the soil of Longgang National Natural Reserve, Guangxi Zhuang Autonomous Region, screening and separating obtains first; this bacterial strain has high salt concentration resistance; detected result show this bacterium be a kind of can the new enterobacter cloacae kind of the difficult molten inorganic phosphorus of high-efficiency dissolution, classification called after enterobacter cloacae NG-33 (Enterobactercloacae).

2. enterobacter cloacae NG-33 provided by the invention, due to its energy high yield gluconic acid, acetic acid, it is possible to the difficult molten phosphorus of high-efficiency dissolution, discharges solvable phosphorus, to increase content of available phosphorus in soil, it is possible to release plant Low phosphorus stress, Promoting plant growth. Its reason is, phosphorus is one of biological important nutritive element, is the important component of protoplasma, is form the indispensable element of high-energy phosphate bond. Biological process in the photosynthesis of plant and body all must have phosphorus to participate in. In agriculture production, as the phosphoric of one of major fertilizer elements, in the soil of the single nitrogenous fertilizer of long-term application, it also it is one of the shortest element. General soil all contains abundant phosphorus source, but the form of difficult molten phosphorus that they are difficult to mainly with plant directly absorb greatly exists. Soil phosphate solubilizing microorganism can dissolve difficult molten phosphorus, and release rapid available phosphorus, meets plant to the demand of phosphoric, play saving phosphate fertilizer, the effect of improvement soil.

3. enterobacter cloacae NG-33 provided by the invention can tryptophane be precursor synthesis plant growth hormones indolylacetic acid (IAA), owing to it is adsorbed on the surface of seed and root system, can directly be utilized by plant, simultaneously also may with the growth of raw IAA acting in conjunction stimulating plant cell in plant itself and propagation, thus promote growing and the effectively moisture in absorption soil and nutrient of root system of plant.The adjustment of possibility involved in plant body other vital movements interior simultaneously.

4. enterobacter cloacae NG-33 provided by the invention, all has significant promoter action to the strain height in corn seedling stage under different Low phosphorus stress, biomass, root system and photosynthetic rate.

Preservation information explanation

Enterobacter cloacae NG-33 (Enterobactercloacae), deposit number is CCTCCM2016056, preservation date is on January 21st, 2016, and depositary institution is China typical culture collection center (CCTCC), and preservation address is wuchang, wuhan road Jia Shan Wuhan University.

Accompanying drawing explanation

Fig. 1 is molten phosphorus circle on NBRIP solid medium of the enterobacter cloacae NG-33 (Enterobactercloacae) of the present invention and colonial morphology;

Fig. 2 is the K of different concns₂HPO₄The solvable phosphorus typical curve that the absorbance of standard substance at 700nm place is drawn out;

Fig. 3 is the gluconic acid typical curve that the gluconic acid standard substance of different concns are drawn out with chromatography of ions absorption peak area;

Fig. 4 is the acetic acid typical curve that the acetic acid standard substance of different concns are drawn out with chromatography of ions absorption peak area;

Fig. 5 is the enterobacter cloacae NG-33 (Enterobactercloacae) of the present invention comparison to over-ground part biomass during corn growth-promoting 24 days in seedling stage in two kinds of soil;

Fig. 6 is the enterobacter cloacae NG-33 (Enterobactercloacae) of the present invention comparison to Underground biomass during corn growth-promoting 24 days in seedling stage in two kinds of soil;

Fig. 7 is the comparison that the enterobacter cloacae NG-33 (Enterobactercloacae) of the present invention is high to strain during corn growth-promoting 24 days in seedling stage in two kinds of soil;

Fig. 8 be the enterobacter cloacae NG-33 (Enterobactercloacae) of the present invention in two kinds of soil to the comparison of content of soil available phosphor.

Embodiment

Below in conjunction with specific embodiment, the present invention is described in further detail, and the explanation of the invention is not limited.

Material used in following embodiment and reagent, if no special instructions, all can obtain from commercial channels. The experimental technique used in following embodiment if no special instructions, is ordinary method.

Embodiment 1: the separation andpreconcentration of enterobacter cloacae NG-33 (Enterobactercloacae)

The separation of 1.1 enterobacter cloacae NG-33 (Enterobactercloacae)

The separation of enterobacter cloacae NG-33 (Enterobactercloacae) comprises sampling, enrichment and just sieve, sieves 3 steps again, specific as follows:

1.1.1 sampling

Sample from the soil of Longgang National Natural Reserve, Guangxi Zhuang Autonomous Region, load in clean sampler bag (bottle), carry out mark, save backup in 4 DEG C of refrigerators.

1.1.2 enrichment and just sieve

The sample taking 10g collection is poured in the liquid substratum of Luria-Bertani (LB) that 100mL contains 800mM, 37 DEG C, 200rpm, shaking culture 24h. Then get 1mL bacteria suspension, it is inoculated in the triangular flask that NBRIP liquid nutrient medium is housed, multiple shaking flask parallel test, be provided with control sample (not inoculating any bacterium). It is placed in 37 DEG C of shaking table 120r/min and cultivates 5 days, measure the content of titanium pigment in nutrient solution, therefrom filter out the shaking flask that dissolving P capacity is stronger.

1.1.3 sieve again

To just sieve the shake flask culture obtained by gradient dilution, get 0.1mL and be applied on the flat board of NBRIP substratum, be inverted and cultivate 4 days, select the bacterial strain separating for several times with bigger molten phosphorus circle and go down to posterity in 37 DEG C of incubators, separation obtains the higher bacterial strain of a strain dissolving P capacity.

The qualification of 1.2 enterobacter cloacae NG-33 (Enterobactercloacae)

The pure culture bacterial strain above-mentioned separation and purification obtained carries out a series of Physiology and biochemistry qualification, and carries out DNA extraction, the amplification of 16SrDNA and order-checking.

1.2.1 this bacterial strain forms on LB solid medium that circular or subcircular, oyster white, projection, surface light are lubricious, the bacterium colony of neat in edge, as shown in Figure 1. Mirror inspection shows peritrichous, without pod membrane, belongs to Gram-negative bacteria, and other test items are as shown in table 1:

Table 1 biochemical reactions result

Physiological and biochemical index	Reaction result
		Arginine hydrolysis experiment	+
Gas is produced by glycerine	–
		Citrate trianion utilizes test	+
Gas is produced by inositol	–
		Gelatin liquification test	+
Lysine decarboxylase	–
		Indole test	+
Methyl red test	+
		Pigment produces test	–
Produce growth hormone (IAA) test	+

Note :+represent positive reaction, exist or have, represent negative reaction, do not exist or do not have.

1.2.2 utilizing primers F 27 and R1492 to carry out amplification 16SrDNA, primer sequence is as follows:

F27:5 '-AGAGTTTGATCATGGCTCAG-3 ';

R1492:5 '-TAGGGTTACCTTGTTACGACTT-3 '.

Pcr amplification condition is 95 DEG C of 5min, 94 DEG C of 30s, 55 DEG C of 30s, 72 DEG C of 90s, 30 circulations, 72 DEG C of 10min.

Pcr amplification product is checked order, sequencing result is as shown in SEQIDNO.1, the amplification length of the 16SrDNA sequence of NG-33 bacterial strain is 1446bp, through sequence analysis, itself and EnterobactercloacaestrainEC7 (GenBankaccessionno.KJ210328.1) have the high similarity of 99%, in conjunction with NG-33 strain morphology and physio-biochemical characteristics, identify that this bacterial strain is the bacterial strain that Enterobactercloacae bacterium belongs to, called after enterobacter cloacae NG-33 (Enterobactercloacae), it is preserved in China typical culture collection center (CCTCC), preserving number is CCTCCM2016056.

Embodiment 2: the mensuration of the solvable phosphorus content of enterobacter cloacae NG-33 (Enterobactercloacae) shake-flask culture

The configuration of the 2.1 anti-colour developing liquid of molybdenum antimony

1. tartrated antimony (K (SbO) C is got₄H₄O₆) 0.5g, it is dissolved in 100mL water.

2. ammonium molybdate ((NH is taken₄)₆Mo₇O₂₄·4H₂O) 10g, it is dissolved in 450mL water, slowly adds the dense H of 153mL₂SO₄, Bian Jiabian stirs.

3. more above-mentioned 1. solution is joined 2. in solution, finally add water to 1L. Fully shaking even, store in brown bottle, this is molybdenum antimony mixed solution.

Before use, 4. left-handed xitix (C is taken₆H₈O₅, chemical pure) and 1.5g, it is dissolved in 100mL molybdenum antimony mixed solution, mixed even, this i.e. the anti-reagent of molybdenum antimony. Validity period 24 hours, as being hidden in refrigerator, then validity period is longer.

Strains tested is in the liquid substratum of 10mLLB, and 30 DEG C, 180rpm, shaking culture 24h, get 1mL nutrient solution and be inoculated in the triangular flask that 100mLNBRIP liquid nutrient medium is housed, and arranges multiple repetition and control group (not connecing bacterium). Being placed in 37 DEG C of shaking table 180r/min and cultivate 5 days, the every day same time gets 5mL fermented liquid, 8000rmp centrifuging and taking supernatant liquor. Experiment adopts molybdenum antimony resistance colorimetric method to measure solvable phosphorus content in fermented liquid, and the fermented supernatant fluid after absorption 1mL is centrifugal adds in colorimetric cylinder, then adds the anti-developer 5mL (H of molybdenum antimony₂SO₄For 5.5mol L^-1, ammonium molybdate is 10g L^-1, tartrated antimony is 0.5g L^-1, xitix is 15g L^-1), adding distil water is settled to 50mL, leaves standstill after 15-20mim, take control group as blank, measure OD value under 650nm, taking concentration as 0,5,10,15,20, the K of 25mg/L₂HPO₄Standard substance do typical curve in the same way, as shown in Figure 2.

Result shows, the solvable phosphorus content of strains tested enterobacter cloacae NG-33 (Enterobactercloacae) shake-flask culture of the present invention is 197mg/L.

Embodiment 3: enterobacter cloacae NG-33 (Enterobactercloacae) produces the mensuration of kinds of organic acids and amount

NG-33 bacterial strain at liquid NBRIP liquid nutrient medium, 37 DEG C, 180rmp/min when, continuing fermentation 5 days, every day, same timing nutrient solution, then carried out the organic acid in ion chromatography fermented liquid. Getting 1mL fermented liquid and be placed in 2mL plastic centrifuge tube, with 12000r/min centrifugation 5min, get supernatant liquor, dilute 10 times with ultrapure water, diluent removes micron order suspended substance through 0.23 μm of membrane filtration. Use ICS-5000 chromatography of ions device (Thmero company of the U.S.), adopt IonPacAS11 anion-exchange column, IC-RP guard column, analytical column (250mm × 4mm), guard column (50mm × 4mm). Adopt gradient elution, flow velocity 1mL/min, post temperature 30 DEG C, sampling volume 25 μ L. Gradient elution program is as shown in table 2, to obtain typical curve as shown in Figure 3-4 after standard specimen gradient dilution.

Table 2 ion chromatography organic acid gradient elution program

Result shows, it is 4889.21mg/L that the enterobacter cloacae NG-33 (Enterobactercloacae) of the present invention produces gluconic acid production peak, and producing acetic acid production peak is 329.08mg/L.

Embodiment 4: the mensuration of enterobacter cloacae NG-33 (Enterobactercloacae) synthetically grown hormone IAA content

Strains tested enterobacter cloacae NG-33 (Enterobactercloacae) is in LB nutrient solution (containing 0.5mg/mL tryptophane), and 37 DEG C, 150rpm shaking culture 24h, bacterium liquid OD is surveyed in sampling₆₀₀, work as OD₆₀₀Thalline is collected when=0.5. Get 2mL supernatant liquor, add 2mLSalkowski reagent (containing the 150mL vitriol oil, 250mLddH₂O and 7.5mL0.5mol/LFeCl₃), after room temperature dark colour developing 20min, survey absorbancy at 535nm wavelength place. Aseptic culture medium is the same to be done identical process and returns to zero in contrast. Doing typical curve (see Fig. 3) taking concentration as the IAA standard substance of 0,10,20,30,40,50,60,70,80 μ g/mL with method, IAA content unit is μ g/mgFW.

Result shows, it is 0.215 μ g/mgFW that the enterobacter cloacae NG-33 (Enterobactercloacae) of the present invention synthesizes the amount of IAA; And detected result also shows that enterobacter cloacae NG-33 can utilize tryptophane to produce IAA.

Embodiment 5: enterobacter cloacae NG-33 (Enterobactercloacae) is to the growth-promoting effect of drought stress corn in lower seedling stage

Adopt filling root mode that corn is carried out Biological control under two kinds of soil. 1 month by a definite date, period watered 3 bacterium liquid, observed the strain height of corn of pouring bacterium liquid, on the ground, the situation that grows compared with the corn not watering bacterium liquid of underground biomass, specifically as illustrated in figs. 5-7.

Two kinds of soil for corn Biological control are respectively yellow moist soil and scarce phosphorus sandy soil, and wherein yellow moist soil takes from agricultural college of Guangxi University experimental plot, arrange 5 sample prescriptions during collected specimens in each region, and sample area is 1 × 0.3m², collect topsoil (0-20cm) and then mix, two kinds of soil through air-dry, pulverize after to cross 100 orders sieves for subsequent use, two kinds of soil manager's voltinism quality detection. The physico-chemical property of yellow moist soil is as follows: pH7.65, alkali-hydrolyzable nitrogen 250.31mg/kg, available phosphorus 24.56mg/kg, available potassium 16.78mg/kg; The physico-chemical property lacking phosphorus sandy soil is as follows: pH5.04, alkali-hydrolyzable nitrogen 190.25mg/kg, available phosphorus 10.41mg/kg, available potassium 11.26mg/kg. It is distributed in the cup of cave in (wide 9cm × high 11cm), every barrel of 0.5Kg.

Enterobacter cloacae NG-33 (Enterobactercloacae) is inoculated in LB liquid nutrient medium, 37 DEG C, 120rpm, shaking culture 18h, detection bacterium liquid OD₆₀₀8000rpm centrifugal collection thalline when=0.9, is resuspended in sterilized water after precipitating 3 times with sterilized water washing, makes final concentration reach 10⁸CFU/ml. It is wash 3 times with sterilized water again after the ethanol surface sterilization 1.5min of 75% by the corn seed volume fraction soaked of spending the night, with the bacterium immersion kind 2h after dilution, evenly plant in the cup of cave, every glass of 2 strains, each control group repeats 10 cave cups, accessing the bacterium liquid 5mL after dilution again, thinning after growing a week, each cave cup stays a strain; Within every three days, supplement and once lack phosphorus Hoagland ' s nutritive medium { Ca (NO₃)₂·4H₂O945mg/L, KNO₃506mg/L, NH₄NO₃80mg/L, MgSO₄·7H₂O493mg/L, iron salt solutions 2.5ml/L (FeSO₄·7H₂O5.56g/L, EDTA-2Na7.45g/L) }. Each connects a bacterium liquid for two weeks, and whole experiment carries out in Life Science and Technology institute of Guangxi University greenhouse, room temperature about 28 DEG C when natural lighting, relative air humidity about 70%.

Measure the corn processed through enterobacter cloacae NG-33 (Enterobactercloacae) after 24 days, compared with the control, strain height, on the ground, underground biomass change as shown in table 3.

Table 3 enterobacter cloacae NG-33 is strain height when corn growth-promoting in seedling stage length 24 days, on the ground, the change of underground biomass

As can be seen from Table 3, the enterobacter cloacae NG-33 (Enterobactercloacae) of the present invention live bacterium at yellow moist soil, lack in phosphorus sandy soil two kinds of soil the strain height in corn seedling stage had significant promoter action.

In addition, the change of the available phosphorus contents of two kinds of soil that mensuration processes through enterobacter cloacae NG-33 (Enterobactercloacae) is as shown in table 4, Fig. 8.

Two kinds of content of available phosphorus in soil changes that table 4 enterobacter cloacae NG-33 processes

As can be seen from Table 4, the available phosphorus contents in yellow moist soil, scarce phosphorus sandy soil two kinds of soil is also increased significantly by the enterobacter cloacae NG-33 (Enterobactercloacae) of the present invention bacterium alive.

In sum, the enterobacter cloacae NG-33 (Enterobactercloacae) of the present invention due to its can high yield gluconic acid, acetic acid, can the difficult molten phosphorus of high-efficiency dissolution, discharge solvable phosphorus, to increase content of available phosphorus in soil, plant Low phosphorus stress can be released, Promoting plant growth. It can also synthesis of indole acetic acid such that it is able to helping plant to provide indolylacetic acid, play the effect of promoting growth of plants, it is to the raising of content of soil available phosphor, can assist improvement soil. Therefore, due to the general character of the promoting growth of plants that above-mentioned effect has, so this bacterium can be applied in the middle of Plant growth-promoting effect as Promoting bacteria widely.

The enterobacter cloacae NG-33 (Enterobactercloacae) of the present invention can promote the growth of plant from many aspects, the enterobacter cloacae NG-33 (Enterobactercloacae) of the present invention processes the strain height of corn, ground and underground biomass and all has significant promoter action, also be significantly increased effect to content of soil available phosphor, can be applicable to the preparation of corn growth-promoting preparation or microbial fertilizer.

The aforementioned description to the concrete exemplary of the present invention is to illustrate and the object of illustration. These descriptions not want to be defined as the present invention disclosed precise forms, and obviously, according to above-mentioned instruction, it is possible to carry out much changing and change.The object exemplary embodiment selected and describe is to explain the certain principles of the present invention and practical application thereof, so that the technician of this area can realize and utilize the various different exemplary of the present invention and various different selection and change. The scope of the present invention is intended to be limited by claim book and equivalents thereof.

Claims

1. separate the plant growth-promoting bacterial strain NG-33 of inorganic phosphorus for one kind, its taxonomy called after enterobacter cloacae NG-33 (Enterobactercloacae), being preserved in China typical culture collection center on January 21st, 2016, preserving number is CCTCCM2016056.

2. the plant growth-promoting bacterial strain NG-33 of solution inorganic phosphorus according to claim 1 is in the purposes increased in soil in solvable phosphorus content.

3. the purposes of the plant growth-promoting bacterial strain NG-33 of solution inorganic phosphorus according to claim 1 in high yield gluconic acid, acetic acid.

4. the plant growth-promoting bacterial strain NG-33 of solution inorganic phosphorus according to claim 1 synthetically grown element IAA in purposes, it is characterised in that, described plant growth-promoting bacterial strain can utilize tryptophane synthetically grown element IAA.

5. the purposes of the plant growth-promoting bacterial strain NG-33 of solution inorganic phosphorus according to claim 1 in Promoting plant growth.

6. the plant growth-promoting bacterial strain NG-33 of solution inorganic phosphorus according to claim 1 is in the purposes improved in content of available phosphorus in soil.

7. the plant growth-promoting bacterial strain NG-33 of solution inorganic phosphorus according to claim 1 is in the purposes prepared in Promoting plant growth preparation.

8. the purposes of the plant growth-promoting bacterial strain NG-33 of solution inorganic phosphorus according to claim 7 in the preparation preparing Promoting plant growth, it is characterised in that, described Promoting plant growth preparation is microbiobacterial agent or microbial fertilizer.