CN106962115A - A kind of method for improving willow resistance against diseases - Google Patents
A kind of method for improving willow resistance against diseases Download PDFInfo
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- CN106962115A CN106962115A CN201710217291.3A CN201710217291A CN106962115A CN 106962115 A CN106962115 A CN 106962115A CN 201710217291 A CN201710217291 A CN 201710217291A CN 106962115 A CN106962115 A CN 106962115A
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- seedling
- bacterium solution
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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Abstract
The present invention relates to a kind of method for improving willow resistance against diseases, following steps are specifically included：（1）Environmental Kuznets Curves；（2）Bacterium solution is prepared；（3）The processing of seedling；（4）Treat seedling length to height 50 60cm can transplant outdoor.By experiment, a kind of treated poplar seedlings of the method for the raising willow resistance against diseases provided using the present invention, its resistance against diseases is significantly improved, 33.1% is up to without disease incidence of the seedling of any processing after outdoor is transplanted in 3 months, and treated seedling disease incidence is reduced to less than 9.6%, effect significantly, effectively reduces the use of disease control medicament, of great advantage to poplar seedlings early growth period.
Description
Technical field
The invention belongs to planting technology field, and in particular to a kind of method of raising willow resistance against diseases.
Background technology
Willow, arbor, trunk generally end is straight, and bark is smooth or lobe, is often canescence；Willow is to be distributed in the world most
Extensively, the forest species of adaptability most strong seeds, scattered temperate zone on the Northern Hemisphere and cool temperature zone, China be distributed in Central China, North China,
The wide area such as northwest, northeast.The breeding method of conventional willow has the method for growing directly from seeds, cuttage, tissue culture method etc., wherein cuttage
It is the most commonly used.
After seedling is cultivated into cutting, seedling is often because the change of environment and spontaneous immune system are weaker, after transplanting
Disease infestation is highly prone to, conventional method sprinkling medical treatment is that is, harmful, is unfavorable for the growth of seedling again.
The content of the invention
In order to solve the above problems, the invention provides a kind of method for improving willow resistance against diseases.
The present invention is achieved through the following technical solutions：
A kind of method for improving willow resistance against diseases, specifically includes following steps：
（1）Environmental Kuznets Curves
Before seedling replanting, during height 25-30cm, environment temperature in greenhouse was improved into the humidity to 25-30 DEG C, canopy in continuous 5-7 days
It is reduced to less than 45%, daily light irradiation time to increase to 14-16 hours, three times a day spray water to seedling blade face, it is ensured that blade face is wet
Profit, can effectively strengthen the metabolism degree and intensity of photosynthesis of small plants cell, accumulate more multi-energy, and the operation after being is done
It is good to prepare；
（2）Bacterium solution is prepared
The sick leaf for just having infected the diseases such as rot, black spot, rust is collected, shreds and is placed in container, plus 1 times of sick leaf quality part
Several mass fractions is 2% glucose solution, is soaked 45-60 minutes in 30-35 DEG C, filtering and impurity removing obtains filtrate, and filtrate added water into 20
Dilute again, be then heated to 60-65 DEG C, be incubated 5-8 minutes, obtain bacterium solution, the bacterium solution contains the fungi of inactivation, bacterium, method
Simply, it is time saving and energy saving；
（3）The processing of seedling
Longitudinal direction in the middle part of the seedling trunk handled well is carved and hinders 1-2cm, quarter hinders depth 0.3-0.5cm, is prevented effectively from and hurt multidimensional
Tube bank, cause seedling development bad, after its formed callus after, to callus at spray step（2）The bacterium solution, altogether spray
Spill twice, 12 hours interval times, first time fountain height 2-3mL, second of fountain height 5-8mL, the bacterium solution is now with the current；Energy
Enough effective stimulus phytoalexins, the secretion and synthesis of plant disease-resistant albumen, reach the resistance energy for improving seedling to Common Diseases
Power, and inactivated bacterial liquid is sprayed to callus, cell metabolic rates are fast at callus, and intensity is big, compared to foliage spray
It is easier to be upset to form a large amount of ill-resistant proteins and phytoalexin, improves its resistance against diseases, it is to avoid do not inactivated in bacterium solution
Pathogenic bacteria directly result in seedling and caught an illness；
（4）Treat that seedling length can transplant outdoor to height 50-60cm, resistance against diseases is significantly improved.
Beneficial effects of the present invention：Before seedling replanting, environment in greenhouse is cultivated in control, can effectively strengthen small plants cell
Metabolism degree and intensity of photosynthesis, accumulate more multi-energy, the ready for operation after being, then by the middle part of seedling trunk
Wound is carved in longitudinal direction, is prevented effectively from and hurts excessive vascular bundle, causes seedling development bad, after it forms callus, to callus group
Knit place's sprinkling step（2）The bacterium solution, can effective stimulus phytoalexin, the secretion and synthesis of plant disease-resistant albumen, reach
Resistivity of the seedling to Common Diseases is improved, and cell metabolism speed at inactivated bacterial liquid, callus is sprayed to callus
Rate is fast, and intensity is big, is easier to be upset to form a large amount of ill-resistant proteins and phytoalexin compared to blade face and root sprinkling, carries
Its high resistance against diseases, it is to avoid the pathogenic bacteria not inactivated in bacterium solution directly result in seedling and caught an illness.By experiment, present invention offer is used
A kind of raising willow resistance against diseases the treated poplar seedlings of method, its resistance against diseases is significantly improved, without any place
Disease incidence of the seedling of reason after outdoor is transplanted in 3 months is up to 33.1%, and treated seedling disease incidence is reduced
To less than 9.6%, effect significantly, effectively reduces the use of disease control medicament, of great advantage to poplar seedlings early growth period.
Embodiment
Embodiment 1
A kind of method for improving willow resistance against diseases, specifically includes following steps：
（1）Environmental Kuznets Curves
Before seedling replanting, during height 25-30cm, environment temperature in greenhouse was improved into 25 DEG C, canopy humidity in continuous 5 days and be reduced to
Less than 45%, daily light irradiation time increases to 14 hours, three times a day seedling blade face is sprayed water, it is ensured that blade face is moistened, and can effectively be increased
The metabolism degree and intensity of photosynthesis of strong small plants cell, accumulate more multi-energy, the ready for operation after being；
（2）Bacterium solution is prepared
The sick leaf for just having infected the diseases such as rot, black spot, rust is collected, shreds and is placed in container, plus 1 times of sick leaf quality part
Several mass fractions be 2% glucose solution, in 30 DEG C soak 45 minutes, filtering and impurity removing obtains filtrate, by filtrate add water 20 times it is dilute
Release, be then heated to 60 DEG C, be incubated 5 minutes, obtain bacterium solution, the bacterium solution contains the fungi of inactivation, bacterium, and method is simple, time saving
It is laborsaving；
（3）The processing of seedling
Longitudinal direction in the middle part of the seedling trunk handled well is carved and hinders 1-2cm, quarter hinders depth 0.3-0.5cm, is prevented effectively from and hurt multidimensional
Tube bank, cause seedling development bad, after its formed callus after, to callus at spray step（2）The bacterium solution, altogether spray
Spill twice, 12 hours interval times, first time fountain height 2-3mL, second of fountain height 5-8mL, the bacterium solution is now with the current；Energy
Enough effective stimulus phytoalexins, the secretion and synthesis of plant disease-resistant albumen, reach the resistance energy for improving seedling to Common Diseases
Power, and inactivated bacterial liquid is sprayed to callus, cell metabolic rates are fast at callus, and intensity is big, compared to foliage spray
It is easier to be upset to form a large amount of ill-resistant proteins and phytoalexin, improves its resistance against diseases, it is to avoid do not inactivated in bacterium solution
Pathogenic bacteria directly result in seedling and caught an illness；
（4）Treat that seedling length can transplant outdoor to height 50-60cm, resistance against diseases is significantly improved.
Embodiment 2
A kind of method for improving willow resistance against diseases, specifically includes following steps：
（1）Environmental Kuznets Curves
Before seedling replanting, during height 25-30cm, environment temperature in greenhouse was improved into 30 DEG C, canopy humidity in continuous 7 days and be reduced to
Less than 45%, daily light irradiation time increases to 16 hours, three times a day seedling blade face is sprayed water, it is ensured that blade face is moistened, and can effectively be increased
The metabolism degree and intensity of photosynthesis of strong small plants cell, accumulate more multi-energy, the ready for operation after being；
（2）Bacterium solution is prepared
The sick leaf for just having infected the diseases such as rot, black spot, rust is collected, shreds and is placed in container, plus 1 times of sick leaf quality part
2% several glucose solutions, soaks 60 minutes, filtering and impurity removing obtains filtrate, and filtrate is added water 20 times and diluted, is then heated in 35 DEG C
To 65 DEG C, 8 minutes are incubated, bacterium solution is obtained, the bacterium solution contains the fungi of inactivation, bacterium, and method is simple, time saving and energy saving；
（3）The processing of seedling
Longitudinal direction in the middle part of the seedling trunk handled well is carved and hinders 1-2cm, quarter hinders depth 0.3-0.5cm, is prevented effectively from and hurt multidimensional
Tube bank, cause seedling development bad, after its formed callus after, to callus at spray step（2）The bacterium solution, altogether spray
Spill twice, 12 hours interval times, first time fountain height 2-3mL, second of fountain height 5-8mL, the bacterium solution is now with the current；Energy
Enough effective stimulus phytoalexins, the secretion and synthesis of plant disease-resistant albumen, reach the resistance energy for improving seedling to Common Diseases
Power, and inactivated bacterial liquid is sprayed to callus, cell metabolic rates are fast at callus, and intensity is big, compared to foliage spray
It is easier to be upset to form a large amount of ill-resistant proteins and phytoalexin, improves its resistance against diseases, it is to avoid do not inactivated in bacterium solution
Pathogenic bacteria directly result in seedling and caught an illness；
（4）Treat that seedling length can transplant outdoor to height 50-60cm, resistance against diseases is significantly improved.
Comparative example 1
This comparative example 1 compared with Example 1, without step（1）The operation, method and step all same in addition.
Comparative example 2
This comparative example 2 compared with Example 2, without step（3）It is described that the callus sprinkling bacterium solution is operated, and
It is, method and step all same in addition described uniform to root sprinkling.
Control group
Without the operation of any its resistance against diseases of raising during growth of seedling.
Same kind, growing way identical poplar seedlings are handled using upper 5 kinds of methods respectively, it is counted and transplants outdoor
Common Diseases incidence in 3 months afterwards, many experiments, as a result such as table 1：
Table 1
| Sample number（Strain） | Disease incidence（%） | |
| Embodiment 1 | 500 | 8.9 |
| Embodiment 2 | 500 | 9.6 |
| Comparative example 1 | 500 | 15.2 |
| Comparative example 2 | 500 | 25.5 |
| Control group | 500 | 33.1 |
As shown in Table 1, a kind of treated poplar seedlings of the method for the raising willow resistance against diseases provided using the present invention, it resists
Sick ability is significantly improved, and 33.1% is up to without disease incidence of the seedling of any processing after outdoor is transplanted in 3 months,
And treated seedling disease incidence is reduced to less than 9.6%, effect significantly, effectively reduces the use of disease control medicament,
It is of great advantage to poplar seedlings early growth period.
Claims (1)
1. a kind of method for improving willow resistance against diseases, it is characterised in that specifically include following steps：
（1）Environmental Kuznets Curves
Before seedling replanting, during height 25-30cm, environment temperature in greenhouse was improved into the humidity to 25-30 DEG C, canopy in continuous 5-7 days
Less than 45%, daily light irradiation time is reduced to increase to 14-16 hours, three times a day spray water to seedling blade face；
（2）Bacterium solution is prepared
The sick leaf for just having infected the diseases such as rot, black spot, rust is collected, shreds and is placed in container, plus 1 times of sick leaf quality part
Several mass fractions is 2% glucose solution, is soaked 45-60 minutes in 30-35 DEG C, filtering and impurity removing obtains filtrate, and filtrate is added water
20 times of dilutions, are then heated to 60-65 DEG C, are incubated 5-8 minutes, obtain bacterium solution, the bacterium solution contains the fungi of inactivation, bacterium；
（3）The processing of seedling
Longitudinal direction in the middle part of the seedling trunk handled well is carved and hinders 1-2cm, quarter hinders depth 0.3-0.5cm, after it forms callus,
Step is sprayed to callus（2）The bacterium solution, sprays twice, 12 hours interval times, first time fountain height 2-3mL altogether,
Second of fountain height 5-8mL, the bacterium solution is now with the current；
（4）Treat that seedling length can transplant outdoor to height 50-60cm.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710217291.3A CN106962115A (en) | 2017-04-05 | 2017-04-05 | A kind of method for improving willow resistance against diseases |
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| CN201710217291.3A CN106962115A (en) | 2017-04-05 | 2017-04-05 | A kind of method for improving willow resistance against diseases |
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| CN106962115A true CN106962115A (en) | 2017-07-21 |
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| CN201710217291.3A Pending CN106962115A (en) | 2017-04-05 | 2017-04-05 | A kind of method for improving willow resistance against diseases |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107409708A (en) * | 2017-08-11 | 2017-12-01 | 合肥润雨农业科技有限公司 | A kind of method for improving okra resistance against diseases |
Citations (4)
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|---|---|---|---|---|
| CN102965296A (en) * | 2012-02-10 | 2013-03-13 | 广西壮族自治区农业科学院微生物研究所 | Nitrogen-fixing Klebsiella sp. with high anti-glyphosate activity and its application |
| CN103146622A (en) * | 2013-03-25 | 2013-06-12 | 黑龙江省农垦科学院农畜产品综合利用研究所 | Paenibacillus terrae strain |
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2017
- 2017-04-05 CN CN201710217291.3A patent/CN106962115A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102965296A (en) * | 2012-02-10 | 2013-03-13 | 广西壮族自治区农业科学院微生物研究所 | Nitrogen-fixing Klebsiella sp. with high anti-glyphosate activity and its application |
| CN103146622A (en) * | 2013-03-25 | 2013-06-12 | 黑龙江省农垦科学院农畜产品综合利用研究所 | Paenibacillus terrae strain |
| CN103141517A (en) * | 2013-03-25 | 2013-06-12 | 黑龙江农垦三龙生物科技有限公司 | Paenibacillus terrae biological agent and application thereof in agriculture |
| CN104694397A (en) * | 2014-12-22 | 2015-06-10 | 吉林农业大学 | Chaetomium globosum and application thereof |
Non-Patent Citations (3)
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| 王锐萍: "新疆甜瓜对瓜类疫霉菌抗性的诱导", 《植物保护》 * |
| 贺英: "杨树溃疡病菌4种变异菌株对毛白杨愈伤组织抗病性的诱导作用", 《西北林学院学报》 * |
| 赵继红: "灰霉菌菌丝提取物诱导番茄抗病性的初步研究", 《河南农业科技》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107409708A (en) * | 2017-08-11 | 2017-12-01 | 合肥润雨农业科技有限公司 | A kind of method for improving okra resistance against diseases |
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Application publication date: 20170721 |
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