CN107094620A - A kind of method of raising Piao Shu seedling resistances against diseases - Google Patents
A kind of method of raising Piao Shu seedling resistances against diseases Download PDFInfo
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- CN107094620A CN107094620A CN201710227355.8A CN201710227355A CN107094620A CN 107094620 A CN107094620 A CN 107094620A CN 201710227355 A CN201710227355 A CN 201710227355A CN 107094620 A CN107094620 A CN 107094620A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to a kind of method of raising Piao Shu seedling resistances against diseases, following steps are specifically included：（1）Seedling environmental Kuznets Curves；（2）Bacterium solution is prepared；（3）Seedling processing；（4）Treat seedling length to height 70 80cm can transplant to outdoor.By experiment, a kind of treated Piao Shu seedlings of the method for the raising Piao Shu resistances against diseases provided using the present invention, its resistance against diseases is significantly improved, 28.6% is up to without disease incidence of the seedling of any processing after outdoor is transplanted in 3 months, and treated seedling disease incidence is reduced to less than 11.1%, effect significantly, effectively reduces the use of disease control medicament, of great advantage initial stage to Piao Shu growth of seedling, the Piao Shu qualities after seedling are higher.
Description
Technical field
The invention belongs to planting technology field, and in particular to a kind of method of raising Piao Shu seedling resistances against diseases.
Background technology
Piao Shu, Ulmaceae Celtis deciduous tree, bark is smooth, grey；Being born in Piao Shu at the resistance to shade in Plain, property light, thermophilic more
Warm moist climate, it is suitable to be born in fertile flat ground.Not tight is required to soil, there is certain Drought resistance, it is also water-fast wet and barren
Soil, adaptive faculty is stronger, is a kind of excellent Landscape Tree Species.
Piao Shu conventional propagation method is method of growing directly from seeds, but seedling is due to the trait segregation between offspring individuals, it is impossible to obtained
The product with maternal uniform quality is obtained, therefore seedling resistance against diseases is very different, is subject to disease infestation.Conventional method pair
Seedling sprinkling chemical agent sterilizing carries out disease control, i.e., harmful, is unfavorable for the growth of seedling again.
The content of the invention
In order to solve the above problems, the invention provides a kind of method of raising Piao Shu seedling resistances against diseases.
The present invention is achieved through the following technical solutions：
A kind of method of raising Piao Shu seedling resistances against diseases, specifically includes following steps：
（1）Seedling environmental Kuznets Curves
After annual beginning of spring nursery, when seedling length is to height of seedling 50-60cm, continuous 8-10 days by environment temperature in seedling raising greenhouse
Degree improves to humidity in 22-28 DEG C, canopy and is reduced to less than 30%, every night increase illumination in 3-4 hours, intensity of illumination 900-1200lx,
Keep blade face to dry, germ reproduction can be prevented on blade face to grow, at the same can effectively strengthen small plants cell metabolism degree and
Intensity of photosynthesis, accumulates more multi-energy, the ready for operation after being；
（2）Bacterium solution is prepared
The sick leaves of Piao Shu for just having infected the diseases such as powdery mildew, sooty mould are collected, shreds and is placed in container, plus 1 times of sick leaf quality part
Several mass fractions is 2% glucose solution, is soaked 30-45 minutes in 28-32 DEG C, filtering and impurity removing obtains filtrate, and filtrate added water into 20
Dilute again, the folium artemisiae argyi for then adding 1 times of sick leaf quality number is heated to 52-58 DEG C jointly, is incubated 8-10 minutes, filters to obtain bacterium
Liquid, the bacterium solution contains the germ of inactivation, and bacterium solution bacteria containing amount is low, and pathogenecity is weak, and preparation method is simple, time saving and energy saving；
（3）Seedling processing
By step（1）Gained seedling longitudinal direction in the middle part of trunk, which is carved, hinders 1-1.5cm, and quarter hinders depth 0.2-0.4cm, is prevented effectively from wound
To excessive vascular bundle, cause seedling development bad, traumatic part to be etched position forms callus, to callus at spray step（2）
The bacterium solution, it is now with the current, spray altogether twice, 12 hours interval times, first time fountain height 2-3mL, second of fountain height 5-
8mL, the bacterium solution is now with the current；Can effective stimulus phytoalexin, the secretion and synthesis of plant disease-resistant albumen, reach raising
Seedling is to the resistivities of Common Diseases, and it is fast to spray cell metabolic rates at inactivated bacterial liquid, callus to callus,
Intensity is big, is easier to be upset compared to foliage spray to form a large amount of ill-resistant proteins and phytoalexin, improves its disease-resistant energy
Power, it is to avoid the pathogenic bacteria not inactivated in bacterium solution directly result in seedling and caught an illness；
（4）Treat that seedling length can be transplanted to outdoor to height 70-80cm, resistance against diseases is significantly improved.
Further, step（1）The intensity of illumination is 1000lx.
Beneficial effects of the present invention：Nursery initial stage, when seedling length is to height of seedling 50-60cm, greenhouse inner ring is cultivated in control
Border, can effectively strengthen the metabolism degree and intensity of photosynthesis of seedling plant cell, accumulate more multi-energy, the operation after being
It is ready, then longitudinal direction in the middle part of seedling trunk is carved and hindered, is prevented effectively from and hurts excessive vascular bundle, seedling development is caused not
It is good, after its formed callus after, to callus at spray step（2）The bacterium solution, can effective stimulus phytoalexin,
The secretion and synthesis of plant disease-resistant albumen, reach the resistivity for improving seedling to Common Diseases, and callus is sprayed
Spill cell metabolic rates at inactivated bacterial liquid, callus fast, intensity greatly, is easier to be upset compared to blade face and root sprinkling
A large amount of ill-resistant proteins and phytoalexin are formed, its resistance against diseases is improved, it is to avoid the pathogenic bacteria not inactivated in bacterium solution directly result in
Seedling catches an illness.A kind of treated Piao Shu seedlings of the method for the raising Piao Shu resistances against diseases provided using the present invention, its is disease-resistant
Ability is significantly improved, and 28.6% is up to without disease incidence of the seedling of any processing after outdoor is transplanted in 3 months,
And treated seedling disease incidence is reduced to less than 11.1%, effect significantly, effectively reduces making for disease control medicament
With of great advantage initial stage to Piao Shu growth of seedling, the Piao Shu qualities after seedling are higher.
Embodiment
Embodiment 1
A kind of method of raising Piao Shu seedling resistances against diseases, specifically includes following steps：
（1）Seedling environmental Kuznets Curves
After annual beginning of spring nursery, when seedling length is to height of seedling 50-60cm, continuous 8 days by environment temperature in seedling raising greenhouse
Improve to humidity in 22 DEG C, canopy and be reduced to less than 30%, increase illumination in 3 hours every night, intensity of illumination 900lx keeps blade face to do
It is dry, it can prevent that germ reproduction is grown on blade face, while the metabolism degree and photosynthesis that can effectively strengthen small plants cell are strong
Degree, accumulates more multi-energy, the ready for operation after being；
（2）Bacterium solution is prepared
The sick leaves of Piao Shu for just having infected the diseases such as powdery mildew, sooty mould are collected, shreds and is placed in container, plus 1 times of sick leaf quality part
2% several glucose solutions, soaks 30 minutes, filtering and impurity removing obtains filtrate, and filtrate is added water 20 times and diluted, 1 is then added in 28 DEG C
The folium artemisiae argyi of sick leaf quality number is heated to 52 DEG C jointly again, is incubated 8 minutes, filters to obtain bacterium solution, the bacterium solution contains the disease of inactivation
Bacterium, and bacterium solution bacteria containing amount is low, pathogenecity is weak, and preparation method is simple, time saving and energy saving；
（3）Seedling processing
By step（1）Gained seedling longitudinal direction in the middle part of trunk, which is carved, hinders 1-1.5cm, and quarter hinders depth 0.2-0.4cm, is prevented effectively from wound
To excessive vascular bundle, cause seedling development bad, traumatic part to be etched position forms callus, to callus at spray step（2）
The bacterium solution, it is now with the current, spray altogether twice, 12 hours interval times, first time fountain height 2-3mL, second of fountain height 5-
8mL, the bacterium solution is now with the current；Can effective stimulus phytoalexin, the secretion and synthesis of plant disease-resistant albumen, reach raising
Seedling is to the resistivities of Common Diseases, and it is fast to spray cell metabolic rates at inactivated bacterial liquid, callus to callus,
Intensity is big, is easier to be upset compared to foliage spray to form a large amount of ill-resistant proteins and phytoalexin, improves its disease-resistant energy
Power, it is to avoid the pathogenic bacteria not inactivated in bacterium solution directly result in seedling and caught an illness；
（4）Treat that seedling length can be transplanted to outdoor to height 70-80cm, resistance against diseases is significantly improved.
Embodiment 2
A kind of method of raising Piao Shu seedling resistances against diseases, specifically includes following steps：
（1）Seedling environmental Kuznets Curves
After annual beginning of spring nursery, when seedling length is to height of seedling 50-60cm, continuous 9 days by environment temperature in seedling raising greenhouse
Improve to humidity in 25 DEG C, canopy and be reduced to less than 30%, increase illumination in 4 hours every night, intensity of illumination 1000lx keeps blade face to do
It is dry, it can prevent that germ reproduction is grown on blade face, while the metabolism degree and photosynthesis that can effectively strengthen small plants cell are strong
Degree, accumulates more multi-energy, the ready for operation after being；
（2）Bacterium solution is prepared
The sick leaves of Piao Shu for just having infected the diseases such as powdery mildew, sooty mould are collected, shreds and is placed in container, plus 1 times of sick leaf quality part
2% several glucose solutions, soaks 40 minutes, filtering and impurity removing obtains filtrate, and filtrate is added water 20 times and diluted, 1 is then added in 30 DEG C
The folium artemisiae argyi of sick leaf quality number is heated to 55 DEG C jointly again, is incubated 9 minutes, filters to obtain bacterium solution, the bacterium solution contains the disease of inactivation
Bacterium, and bacterium solution bacteria containing amount is low, pathogenecity is weak, and preparation method is simple, time saving and energy saving；
（3）Seedling processing
By step（1）Gained seedling longitudinal direction in the middle part of trunk, which is carved, hinders 1-1.5cm, and quarter hinders depth 0.2-0.4cm, is prevented effectively from wound
To excessive vascular bundle, cause seedling development bad, traumatic part to be etched position forms callus, to callus at spray step（2）
The bacterium solution, it is now with the current, spray altogether twice, 12 hours interval times, first time fountain height 2-3mL, second of fountain height 5-
8mL, the bacterium solution is now with the current；Can effective stimulus phytoalexin, the secretion and synthesis of plant disease-resistant albumen, reach raising
Seedling is to the resistivities of Common Diseases, and it is fast to spray cell metabolic rates at inactivated bacterial liquid, callus to callus,
Intensity is big, is easier to be upset compared to foliage spray to form a large amount of ill-resistant proteins and phytoalexin, improves its disease-resistant energy
Power, it is to avoid the pathogenic bacteria not inactivated in bacterium solution directly result in seedling and caught an illness；
（4）Treat that seedling length can be transplanted to outdoor to height 70-80cm, resistance against diseases is significantly improved.
Comparative example 1
This comparative example 1 compared with Example 1, without step（1）The operation, method and step all same in addition.
Comparative example 2
This comparative example 2 compared with Example 2, without step（3）It is described that the callus sprinkling bacterium solution is operated, and
It is, method and step all same in addition described uniform to root sprinkling.
Control group
Without the operation of any its resistance against diseases of raising during growth of seedling.
Same kind, growing way identical Piao Shu seedlings are not handled using upper 5 kinds of methods, it is counted and transplants outdoor
Common Diseases incidence in 3 months afterwards, many experiments, as a result such as table 1：
Table 1
| Sample number（Strain） | Disease incidence（%） | |
| Embodiment 1 | 300 | 10.5 |
| Embodiment 2 | 300 | 11.1 |
| Comparative example 1 | 300 | 16.4 |
| Comparative example 2 | 300 | 19.8 |
| Control group | 300 | 28.6 |
As shown in Table 1, a kind of treated Piao Shu seedlings of the method for the raising Piao Shu resistances against diseases provided using the present invention, its
Resistance against diseases is significantly improved, and is up to without disease incidence of the seedling of any processing after outdoor is transplanted in 3 months
28.6%, and treated seedling disease incidence is reduced to less than 11.1%, effect significantly, effectively reduces disease control medicine
The use of agent, of great advantage initial stage to Piao Shu growth of seedling, the Piao Shu qualities after seedling are higher.
Claims (2)
1. a kind of method of raising Piao Shu seedling resistances against diseases, it is characterised in that specifically include following steps：
（1）Seedling environmental Kuznets Curves
After annual beginning of spring nursery, when seedling length is to height of seedling 50-60cm, continuous 8-10 days by environment temperature in seedling raising greenhouse
Degree improves to humidity in 22-28 DEG C, canopy and is reduced to less than 30%, every night increase illumination in 3-4 hours, intensity of illumination 900-1200lx,
Blade face is kept to dry；
（2）Bacterium solution is prepared
The sick leaves of Piao Shu for just having infected the diseases such as powdery mildew, sooty mould are collected, shreds and is placed in container, plus 1 times of sick leaf quality part
Several mass fractions is 2% glucose solution, is soaked 30-45 minutes in 28-32 DEG C, filtering and impurity removing obtains filtrate, and filtrate added water into 20
Dilute again, the folium artemisiae argyi for then adding 1 times of sick leaf quality number is heated to 52-58 DEG C jointly, is incubated 8-10 minutes, filters to obtain bacterium
Liquid；
（3）Seedling processing
By step（1）Gained seedling longitudinal direction in the middle part of trunk, which is carved, hinders 1-1.5cm, hinders depth 0.2-0.4cm, traumatic part position to be etched quarter
Formed callus, to callus at spray step（2）The bacterium solution, now with the current, twice, interval time 12 is small for sprinkling altogether
When, first time fountain height 2-3mL, second of fountain height 5-8mL；
（4）Treat that seedling length can be transplanted to outdoor to height 70-80cm.
2. a kind of method of raising Piao Shu seedling resistances against diseases according to claim 1, it is characterised in that：Step（1）
The intensity of illumination is 1000lx.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710227355.8A CN107094620A (en) | 2017-04-10 | 2017-04-10 | A kind of method of raising Piao Shu seedling resistances against diseases |
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| CN201710227355.8A CN107094620A (en) | 2017-04-10 | 2017-04-10 | A kind of method of raising Piao Shu seedling resistances against diseases |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112806260A (en) * | 2021-01-20 | 2021-05-18 | 云南帅豪农牧科技有限公司 | Tissue culture seedling raising method for baby cabbage with high disease resistance |
| CN112806261A (en) * | 2021-01-20 | 2021-05-18 | 云南帅豪农牧科技有限公司 | High-disease-resistance tissue culture seedling raising method for Chinese cabbage |
| CN112806259A (en) * | 2021-01-20 | 2021-05-18 | 云南帅豪农牧科技有限公司 | High-disease-resistance polygonatum sibiricum tissue culture seedling method |
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| CN104170645A (en) * | 2014-09-11 | 2014-12-03 | 徐华龙 | Method for cultivating strong high-quality hackberry saplings |
| CN105850726A (en) * | 2016-04-08 | 2016-08-17 | 常州润达科技信息咨询有限公司 | Stem rot resistant new gingko variety selecting and breeding method |
| CN106258770A (en) * | 2016-09-28 | 2017-01-04 | 博白县大红火龙果专业合作社 | A kind of processing method of the anti-disease pest of Hylocereus undatus seedling |
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Patent Citations (3)
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|---|---|---|---|---|
| CN104170645A (en) * | 2014-09-11 | 2014-12-03 | 徐华龙 | Method for cultivating strong high-quality hackberry saplings |
| CN105850726A (en) * | 2016-04-08 | 2016-08-17 | 常州润达科技信息咨询有限公司 | Stem rot resistant new gingko variety selecting and breeding method |
| CN106258770A (en) * | 2016-09-28 | 2017-01-04 | 博白县大红火龙果专业合作社 | A kind of processing method of the anti-disease pest of Hylocereus undatus seedling |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112806260A (en) * | 2021-01-20 | 2021-05-18 | 云南帅豪农牧科技有限公司 | Tissue culture seedling raising method for baby cabbage with high disease resistance |
| CN112806261A (en) * | 2021-01-20 | 2021-05-18 | 云南帅豪农牧科技有限公司 | High-disease-resistance tissue culture seedling raising method for Chinese cabbage |
| CN112806259A (en) * | 2021-01-20 | 2021-05-18 | 云南帅豪农牧科技有限公司 | High-disease-resistance polygonatum sibiricum tissue culture seedling method |
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Application publication date: 20170829 |