CN106171579A - External source brassinosteroid processes the method alleviating the injury of corn seedling cadmium environment stress - Google Patents
External source brassinosteroid processes the method alleviating the injury of corn seedling cadmium environment stress Download PDFInfo
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- CN106171579A CN106171579A CN201610563432.2A CN201610563432A CN106171579A CN 106171579 A CN106171579 A CN 106171579A CN 201610563432 A CN201610563432 A CN 201610563432A CN 106171579 A CN106171579 A CN 106171579A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/22—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
Abstract
A kind of external source brassinosteroid processes the method alleviating the injury of corn seedling cadmium environment stress, plants after specifically including that seed treatment；Choose and grow fine and corn seedling 120 strain that growing way is consistent carries out Cd stress test；If series Cd2+Stress concentration gradient, carries out Hoagland nutritional solution and processes corn seedling, according to seedling relative growth rate after cultivation 10d, filter out optimal Cd2+Stress concentration；Brassinosteroid is to Cd2+Injury relieving effect test arranges 6 and processes solution, processes solution to having planted corresponding 6 of corn seedling pouring；After processing 7d, corn seedling is carried out the mensuration of indices；Finally carry out interpretation of result and the suitableeest EBR concentration screening.External source brassinosteroid can be effectively facilitated heavy metal cadmium and coerce the growth of lower corn seedling, increase the accumulation of corn seedling Biomass, improve heavy metal cadmium and coerce the activities of antioxidant enzymes in lower maize leaf, reduce its activity in vivo oxygen content, reduce and coerce lower Electrolyte Leakage, thus alleviate heavy metal cadmium and coerce the injury to plant.
Description
Technical field
The invention belongs to agriculture applied technology field, be specifically related to external source 24-epi-brassinolide and process heavy metal cadmium
The remission method of growth of maize under environment stress.
Background technology
Cadmium (Cd) is plant non-essential element, has higher mobility and toxicity, has latent to various biologic artifacts
Harm, the disease that the mankind are a lot of can be caused, be considered to be one of heavy metal most threatening to the mankind.Along with mining, smelting
Use containing a large amount of of the fertilizer of heavy metal, chemical fertilizer etc. on refining and Cadmium treated industrial expansion and agricultural production, Cadmium Pollution in Soils
The most serious.Research shows, cadmium accumulation in soil is very big to the harm of plant, and cadmium is accumulated to necessarily containing after entering plant
Amount can destroy plant cell membrane structure and function, causes plant oxidative stress, chlorophyll, sugar and protein synthesis to be obstructed, suppress
Photosynthetic and respiratory, reduces the active of enzyme and some other physiological metabolism thing disorder eventually results in Biomass and yield
Decline.
And, cadmium can also result in Crop yield and quality and declines, can be by the health of the food chain harm mankind.Cause
This, the protective measure that research plant cadmium is poisoned is significant to agricultural sustainable development.
Semen Maydis is to plant one of widest cereal crops on heavy-metal contaminated soil, delays Semen Maydis heavy metallic poison
The existing report alleviated about some hormones of solution method, such as salicylic acid and nitric oxide, and alleviates a huge sum of money about brassinosteroid
The method belonging to environment stress rarely has report, applies rare in actual agricultural production.Brassinosteroid is first and is separated
Active Brassica campestris L element steroid (Brassinosteroids, BRs), be that to be known as activity in the world the highest wide
Spectrum, nontoxic auxin, particularly significant to growth and development of plants.Plant physiologist is thought, it can be to the full extent
Excite in plant at potential, there is promotion plant growth, improve sugar content, increase yield and strengthen cold, drought, flood, salt etc. many
Planting the resistivity of adverse circumstance, have been widely used on producing, the high activity brassinosteroid analogs of synthetic is 24-table oil
Rape lactone (EBR).Therefore inquire into the impact of growth of maize under external source EBR heavy metal Cd stress, specify EBR induction
The possible physiological mechanism of corn seedling preventing from heavy metal Cd stress is very important.
Summary of the invention
The technical problem to be solved in the present invention is that providing one to be processed by external source brassinosteroid alleviates corn seedling
The method of cadmium environment stress injury；The method is simple and easy to do, is not subject to seasonal restrictions, and is possible not only to shorten experimental period, it is also possible to
Improve the tolerance of corn seedling preventing from heavy metal cadmium, it is possible to alleviate the heavy metal cadmium stress damage to growth of maize.
In order to solve above-mentioned technical problem, the present invention adopts the following technical scheme that: a kind of external source brassinosteroid processes slow
The method solving the injury of corn seedling cadmium environment stress, comprises the steps:
1, material prepares: prepare corn seed；The 24-epi-brassinolide aqueous solution of differently configured concentration and cadmium nitrate solution.
2, seed treatment: choose that color and luster is normal, the corn seed of full seed, neat and consistent, with the sodium hypochlorite of 0.5%
Sterilize 10 min, distilled water flushing 3～5 times, blots attached water with filter paper, obtains Semen Maydis disinfection seed.
3, examination material cultivate: Semen Maydis disinfection seed is seeded into be lined with two layers of filter paper germination box in, often 50, germination box,
Germination box length × width × height is 13 cm × 12, cm × 19 cm；It is placed in 25 DEG C of incubators germination；After emergence of corn, choose life
The seedling of long consistent SANYE one heart stage is transferred in the plastic flowerpot of diameter 8 cm × a height of 25 cm, built-in Vermiculitum, and every basin is planted
Seedling 8 strain, in artificial climate indoor cellar culture, every 2d Hoaglands nutritional solution of 1/5 concentration waters, and treats that the 4th leaf is long
After going out, choose and grow fine and corn seedling 120 strain that growing way is consistent carries out Cd stress test.
4, test processes:
4.1 set series Cd2+Stress concentration gradient, carries out Hoagland nutritional solution and processes corn seedling, according to children after cultivation 10d
Seedling relative growth rate, filters out optimal Cd2+Stress concentration；The present invention takes 0 mg L-1 Cd2+、25 mg•L-1 Cd2+、50
mg•L-1 Cd2+、100 mg•L-1 Cd2+、150 mg•L-1 Cd2+With 200mg L-1 Cd2+Six Cd2+Stress concentration.
4.2, according to the result of the test of step 4.1, choose 100mg L-1 Cd2+As stress conditions, brassinosteroid solution
Concentration be set as five concentration: 0,0.004%, 0.008%, 0.012% and 0.016%；Brassinosteroid is to Cd2+Injury relieving is imitated
Should test and 6 process solution are set:
A.CK compares, Hoagland solution；
B. containing 100 mg L-1 Cd2+Hoagland solution；
C. containing 100 mg L-1 Cd2+Hoagland solution with 0.004% brassinosteroid；
D. containing 100 mg L-1 Cd2+With 0.008% brassinosteroid Hoagland solution；
E. containing 100 mg L-1 Cd2+With 0.012% brassinosteroid Hoagland solution；
F. containing 100 mg L-1 Cd2+With 0.016% brassinosteroid Hoagland solution；
The step 3 every basin of gained corn seedling is stayed 5 strains, each process 4 basin, the next day in the plastic flowerpot having planted corn seedling
Water corresponding 6 and process solution, water to flowerpot slightly treatment fluid and ooze out；After processing 7d, corn seedling is entered step
5。
5, the mensuration of indices: rinsed well by step 4 gained corn seedling deionized water, measures plant height, root length
After plant fresh weight, it is placed in 105 DEG C of 30min that complete in baking oven, then is placed in 80 DEG C of baking ovens and dries to constant weight, measure plant and do
Weight；Take second functional leaf of corn seedling and carry out blade relative conductivity, malonaldehyde (MDA) content, superoxide dismutase
(POD), peroxidase (SOD), catalase (CAT) activity, soluble sugar content, soluble protein content, proline
Content and the mensuration of chlorophyll content.
6, interpretation of result and the suitableeest EBR concentration screening: the Cd stress recording step 5 processes in each sample of seedling
Each item data, use Microsoft Excel 2003 software carry out data process, all test data statistical analysiss
SPSS16.0 process, result of the test meansigma methods ± standard deviation (SD) represents, uses Duncan ' s duncan's new multiple range method
Carry out multiple comparisons, each process is carried out significant difference analysis；Optimal EBR concentration is screened according to the index of correlation measured, and
Analyze the impact of growth of maize under EBR heavy metal Cd stress.
In above-mentioned Stress treatment, step 3 box that germinates disinfects in alcohol, and filter paper uses after carrying out autoclaving；For terrible
To preferably coercing effect, seed, after step 2 is sterilized, should immediately enter step 3.It will be apparent to one skilled in the art that: step
Cd in 4.12+The setting of stress concentration gradient, can adjust with designed, designed, and in order to clearly state the present invention, step 4.1 is write
Understand Cd2+Stress concentration gradient；EBR concentration in step 4.2 can make the appropriate adjustments with the change of cadmium concentration in processing, it is possible to
The change of corn variety adjusts, in order to clearly state the variable concentrations having write EBR solution in the present invention, step 4.2 exactly.
The present invention, by inquiring into the impact of growth of maize under external source brassinosteroid heavy metal Cd stress, reflects
EBR has mitigation to the stress damage of growth of seedling under adverse circumstance.The method is simple and easy to do, is not subject to seasonal restrictions, and not only may be used
To shorten test period, it is also possible to improve the tolerance of corn seedling preventing from heavy metal cadmium, it is possible to alleviate heavy metal cadmium to Semen Maydis children
The stress damage of Seedling growth.
The present invention mainly by designing multiple EBR concentration, carry out heavy metal cadmium coerce lower EBR to the POD of corn seedling,
The mensuration of the indexs such as SOD, CAT, MDA, relative conductivity, inquires into growth of maize under external source EBR heavy metal Cd stress raw
The impact of physiological-biochemical characteristic, specifies the possible physiological mechanism of EBR inducing maize seedling preventing from heavy metal cadmium, to alleviating adverse circumstance to plant
Injury, has the highest using value, present invention demonstrates that employing xenobiotics's EBR process is expected to become in practice and improves Semen Maydis and exist
Heavy metal cadmium coerces the good method of lower patience, and application potential is the biggest.
Detailed description of the invention
Method used in the following embodiment of the present invention, if no special instructions, is conventional method；Equipment, reagent used
It is conventional reagent, equipment that Reagent Company buys.
Experimental example, 24-epi-brassinolide processes the screening technique of the suitableeest EBR concentration under heavy metal Cd stress, by with
Lower step is carried out:
1, material prepares: corn hybrid seed " Zheng Dan 958 " seed, 24-table Brassica campestris L element class lactone and cadmium nitrate solution allocation.
2, seed treatment: choose that color and luster is normal, the corn seed of full seed, neat and consistent, corn seed is with 0.5%
Hypochlorite disinfectant 10 min, with distilled water flushing 4 times, blots attached water with aseptic filter paper, obtains Semen Maydis disinfection seed.
3, examination material is cultivated: the corn seed after processing is seeded into the culture dish of a diameter of 15 cm being lined with two layers of filter paper
In, it is placed in dark germination in 25 DEG C of incubators.After emergence of corn, the seedling choosing growth consistent SANYE one heart stage transfers to plastics
In flowerpot, every basin plants seedlings 8 strains, and in artificial climate indoor cellar culture, every 2d Hoaglands nutritional solution of 1/5 concentration waters
Fill, after the 4th leaf grows, choose the corn seedling consistent with growing way that grow fine and carry out Cd stress test.
4, test processes:
First 4.1 tests set series Cd2+(0、25、50、100、150、200mg•L-1) stress concentration gradient, carry out Hoagland
Nutritional solution processes corn seedling, according to seedling relative growth rate after cultivation 10d, filters out optimal Cd2+Stress concentration；This test
Example takes 0 mg L-1 Cd2+、25 mg•L-1 Cd2+、50 mg•L-1 Cd2+、100 mg•L-1 Cd2+、150 mg•L-1 Cd2+With
200mg•L-1 Cd2+Six Cd2+Stress concentration.Result of the test is shown in Table 1.
As shown in Table 1: with variable concentrations (25,50,100,150,200 mg L-1) Cd process corn seedling, its strain
Height, overground part and underground part fresh weight and dry weight are the most on a declining curve, compared with the control, when Cd concentration is 100 mg L-1, plant height,
Fresh weight, on the ground dry weight, underground fresh weight and underground dry weight are all remarkably decreased on the ground, have dropped 54.75% respectively, 57.52%, 30.0%,
47.92% and 31.43%.
In table: different lower cases represent between different disposal notable at 0. 05 level differences, lower same.
4.2 are appreciated that according to the result of the test of step 1: when Cd concentration is 100 mg L-1, corn seedling plant height,
Upper fresh weight, on the ground dry weight, underground fresh weight and underground dry weight are all decreased obviously, and compared with the control, all reach significant level；
Therefore select 100 mg L-1Cd2+0,0.004% stress concentration, the concentration of brassinosteroid solution is set as 5:,
0.008%, 0.012% and 0.016%；Brassinosteroid is to Cd2+Injury relieving effect test arrange 6 process solution:
(1) CK comparison, Hoagland solution；
(2) containing 100 mg L-1 Cd2+Hoagland solution；
(3) containing 100 mg L-1 Cd2+Hoagland solution with 0.004% brassinosteroid；
(4) containing 100 mg L-1 Cd2+With 0.008% brassinosteroid Hoagland solution；
(5) containing 100 mg L-1 Cd2+With 0.012% brassinosteroid Hoagland solution；
(6) containing 100 mg L-1 Cd2+With 0.016% brassinosteroid Hoagland solution.
The step 3 every basin of gained corn seedling is stayed 5 strains, each process solution 4 basin, the next day to having planted moulding of corn seedling
Water corresponding 6 in material flowerpot and process solution, water to flowerpot slightly treatment fluid and ooze out；By corn seedling after process 7d
Enter step 5.
For the ion concentration relative constancy ensured during processing in culture fluid, with being equivalent to Vermiculitum in basin every time when processing
Vermiculitum in the treatment fluid pouring plastic flowerpot that maximum water-holding capacity is 2 times, allows unnecessary solution flow out from water seepage hole, reduces at test
Reason error.
5. the mensuration of indices: rinsed well by the process corn seedling deionized water of gained in step 4, measures strain
After height, root length, plant fresh weight, put 105 DEG C of 30min that complete in baking oven, dry in 80 DEG C of baking ovens to constant weight, measure plant and do
Weight；Taking second functional leaf of corn seedling and carry out indices mensuration, indices includes blade relative conductivity, MDA；POD、
SOD, CAT activity；Soluble sugar, soluble protein, proline and chlorophyll content.Measurement result is as follows:
(1) brassinosteroid processes and the impact of growth of maize under Cd stress is shown in Table 2；By table 2 it follows that with to photograph
Ratio, after independent Cd stress processes, Seedling Biomass all has significance to decline, and after adding the brassinosteroid of variable concentrations, respectively processes
The most independent Cd stress processes and has risen, and shows that brassinosteroid can alleviate the cadmium poisoning impact on growth of maize,
By different disposal significant difference, 0.012% optimum concentration alleviated for brassinosteroid.
(2) brassinosteroid processes and the impact of corn seedling physiological property under Cd stress is shown in Table 3；Can be seen by table 3
Going out: during heavy metal cadmium individual processing, MDA content and relative conductivity are all remarkably higher than (CK) processed without Cd stress；POD、
CAT, SOD activity；Proline, soluble sugar, soluble protein and chlorophyll content are all substantially less than without Cd stress process
(CK), Cd2++ EBR can reduce the content of MDA and relative conductivity, and can improve POD, CAT, SOD activity further；Increase
Add proline, soluble sugar, soluble protein and chlorophyll content, wherein best with 0.012% EBR treatment effect, with a huge sum of money
Belonging to the independent Stress treatment of cadmium to compare, MDA content and relative conductivity reduce 29.89% and 39.39%；The work of POD, CAT and SOD
Property add 9.43%, 13.33 % and 67.21% respectively, proline, soluble sugar, soluble protein and chlorophyll content are respectively
Add 79.45%, 127.27%, 86.78 and 49.37 %.
6. interpretation of result and the suitableeest EBR concentration screening: the Cd stress recording step 5 processes in each sample of seedling
Each item data, use Microsoft Excel 2003 software carry out data process, all test data statistical analysiss
SPSS16.0 process, result of the test meansigma methods ± standard deviation (SD) represents, uses Duncan ' s duncan's new multiple range method
Carry out multiple comparisons, each process is carried out significant difference analysis.
From above example it can be seen that external source 24-epi-brassinolide can be effectively facilitated heavy metal cadmium coerces lower Semen Maydis
The growth of seedling, increases the accumulation of corn seedling Biomass, and raising heavy metal cadmium is coerced the antioxidase in lower maize leaf and lived
Property, reduce its activity in vivo oxygen content, reduce and coerce lower Electrolyte Leakage, thus alleviate heavy metal cadmium and coerce the wound to plant
Evil.Use xenobiotics EBR to process the most easily to operate, effectual, it is expected to become in practice and is effectively improved corn seed germination
Rate, and improve Semen Maydis and coerce the good method of lower patience at heavy metal cadmium, application potential is the biggest.
Claims (1)
1. external source brassinosteroid processes the method alleviating the injury of corn seedling cadmium environment stress, comprises the steps:
(1), material prepares: prepare corn seed；The 24-epi-brassinolide aqueous solution of differently configured concentration and cadmium nitrate are molten
Liquid；
(2), seed treatment: choose that color and luster is normal, the corn seed of full seed, neat and consistent, disappear with the sodium hypochlorite of 0.5%
Poison 10 min, distilled water flushing 3～5 times, blot attached water with filter paper, obtain Semen Maydis disinfection seed；
(3), examination material cultivate: Semen Maydis disinfection seed is seeded into be lined with two layers of filter paper germination box in, often 50, germination box, send out
Bud box length × width × height is 13 cm × 12, cm × 19 cm；It is placed in 25 DEG C of incubators germination；After emergence of corn, choose growth
The seedling of consistent SANYE one heart stage is transferred in the plastic flowerpot of diameter 8 cm × a height of 25 cm, built-in Vermiculitum, and every basin plants seedlings
8 strains, in artificial climate indoor cellar culture, every 2d Hoaglands nutritional solution of 1/5 concentration waters, and treats that the 4th leaf grows
After, choose and grow fine and corn seedling 120 strain that growing way is consistent carries out Cd stress test；
(4), test processes:
4.1 set series Cd2+Stress concentration gradient, carries out Hoagland nutritional solution and processes corn seedling, according to seedling after cultivation 10d
Relative growth rate, filters out optimal Cd2+Stress concentration；The present invention takes 0 mg L-1 Cd2+、25 mg•L-1 Cd2+、50
mg•L-1 Cd2+、100 mg•L-1 Cd2+、150 mg•L-1 Cd2+With 200mg L-1 Cd2+Six Cd2+Stress concentration；
4.2, according to the result of the test of step 4.1, choose 100mg L-1 Cd2+As stress conditions, brassinosteroid solution dense
Spend and be set as five concentration: 0,0.004%, 0.008%, 0.012% and 0.016%；Brassinosteroid is to Cd2+Injury relieving effect is tried
Test and 6 process solution be set:
A.CK compares, Hoagland solution；
B. containing 100 mg L-1 Cd2+Hoagland solution；
C. containing 100 mg L-1 Cd2+Hoagland solution with 0.004% brassinosteroid；
D. containing 100 mg L-1 Cd2+With 0.008% brassinosteroid Hoagland solution；
E. containing 100 mg L-1 Cd2+With 0.012% brassinosteroid Hoagland solution；
F. containing 100 mg L-1 Cd2+With 0.016% brassinosteroid Hoagland solution；
By every basin 5 strain of step (3) gained corn seedling, each process solution 4 basin, the next day to the plastic flower having planted corn seedling
Water corresponding 6 in basin and process solution, water to flowerpot slightly treatment fluid and ooze out；After processing 7d, corn seedling is entered
Step (5)；
(5), the mensuration of indices: step (4) gained corn seedling deionized water is rinsed well, measures plant height, root length
After plant fresh weight, it is placed in 105 DEG C of 30min that complete in baking oven, then is placed in 80 DEG C of baking ovens and dries to constant weight, measure plant and do
Weight；Take second functional leaf of corn seedling and carry out blade relative conductivity, MDA content, POD, SOD, CAT activity, soluble sugar
Content, soluble protein content, proline content and the mensuration of chlorophyll content；
(6), interpretation of result and the suitableeest EBR concentration screening: the Cd stress recording step (5) processes in each sample of seedling
Each item data, use Microsoft Excel 2003 software carry out data process, all test data statistical analysiss
SPSS16.0 process, result of the test meansigma methods ± standard deviation (SD) represents, uses Duncan ' s duncan's new multiple range method
Carry out multiple comparisons, each process is carried out significant difference analysis；Index of correlation screening according to the growth of maize measured
Optimal EBR concentration, and analyze the EBR impact on corn seedling.
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| CN106665311A (en) * | 2016-12-30 | 2017-05-17 | 中国农业科学院麻类研究所 | Method for enhancing cadmium tolerance of hibiscus cannabinus seedlings |
| CN106804153A (en) * | 2017-01-22 | 2017-06-09 | 甘肃农业大学 | The application process that different external source Auto-regulators are alleviated to Course of Corn Seed Germination under low temperature stress and seedling |
| CN106804153B (en) * | 2017-01-22 | 2020-06-23 | 甘肃农业大学 | Application method of different exogenous regulating substances to corn seed germination and seedling alleviation under low-temperature stress |
| CN107271351A (en) * | 2017-06-14 | 2017-10-20 | 北京农学院 | Calcium ion assay method in a kind of petal cell |
| CN107047264A (en) * | 2017-06-21 | 2017-08-18 | 中国农业科学院农业环境与可持续发展研究所 | A kind of method that arsenic low absorption crop is quickly screened using segmentation water culture |
| CN107047264B (en) * | 2017-06-21 | 2019-09-10 | 中国农业科学院农业环境与可持续发展研究所 | A method of arsenic low absorption crop is quickly screened using segmentation water culture |
| CN107155672A (en) * | 2017-07-03 | 2017-09-15 | 中国计量大学 | Method for adjusting and controlling rice heavy metal cadmium accumulation |
| CN109644723A (en) * | 2019-01-25 | 2019-04-19 | 四川大学 | A method of arabidopsis is improved to heavy metal cadmium tolerance |
| CN109940031A (en) * | 2019-03-20 | 2019-06-28 | 浙江大学 | Witchweed lactone is alleviating Cd stress to the application in plant generation cadmium poisoning |
| CN110338024A (en) * | 2019-07-09 | 2019-10-18 | 天津大学 | Brassinosteroid and salicylic composition alleviate application of the cadmium to tobacco oxidative stress |
| CN113057079A (en) * | 2021-03-12 | 2021-07-02 | 西南大学 | Method for improving waterlogging tolerance of sweet sorghum in seedling stage |
| CN114946624A (en) * | 2022-05-18 | 2022-08-30 | 北京市农林科学院 | Method for improving cadmium resistance of iris lactea in seedling stage |
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