CN110338024A - Brassinosteroid and salicylic composition alleviate application of the cadmium to tobacco oxidative stress - Google Patents
Brassinosteroid and salicylic composition alleviate application of the cadmium to tobacco oxidative stress Download PDFInfo
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- CN110338024A CN110338024A CN201910614195.1A CN201910614195A CN110338024A CN 110338024 A CN110338024 A CN 110338024A CN 201910614195 A CN201910614195 A CN 201910614195A CN 110338024 A CN110338024 A CN 110338024A
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- brassinosteroid
- tobacco
- salicylic
- cadmium
- composition
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/45—Tobacco
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- Life Sciences & Earth Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses brassinosteroid and salicylic compositions to alleviate application of the cadmium to tobacco oxidative stress, and the brassinosteroid and salicylic composition are 1:(0.7-7.2 in mass ratio) ratio be made of brassinosteroid and salicylic acid.Composition of the invention is pollution-free, does not injure to plant.Cost is relatively low for the composition of invention, acts on experiments have shown that brassinosteroid and salicylic composition alleviate cadmium tobacco oxidative stress.
Description
Technical field
The present invention relates to phytobiology fields, and in particular to a kind of brassinosteroid and salicylic composition alleviate cadmium
Application to tobacco oxidative stress.
Background technique
Cadmium (Cd) is the strongest heavy metal of bio-toxicity, and referred to as " first of the five poisonous creatures: scorpion, viper, centipede, house lizard, toad ", poisoning is being currently one more next
Severeer agricultural and environmental problem.With the development of industrialization and urbanization, agricultural land soil and the accumulation of heavy metal in water cadmium are super
Mark problem is got worse, and the pollutant containing cadmium enters soil by all means, and Cd is not plant growth necessary element, by
In its hydrophily, it is easy in plant interior accumulation.A large number of studies show that heavy metal Cd can seriously damage plant metabolism, such as reduce
Photosynthetic rate, inhibition Chlorophyll synthesis, lipid peroxidation, enhancing or inhibition activities of antioxidant enzymes etc..
Brassinosteroid (BR) is widely distributed in plant, is primarily present in colored and immature seed, in plant
In growth and development process, the minor change of content can all be had an important influence on plant growth and development, the 16th international plant
BR is identified as the sixth-largest plant hormone in object growth substance meeting.Studies have shown that BR can promote plant cell elongation, cell
Division can be excited sufficiently in plant in potential, promoted plant growth and increased crop yield, improve the cold resistance of plant, improve
Disease-resistant, the saline-alkaline tolerance of plant enhance the resistance of reverse of plant, can reduce injury of the chemical agents such as herbicide to plant.
Salicylic acid (SA) is a kind of phenolic compound generally existing in plant, is intracellular signal transmitting molecule,
There are a variety of Physiological effect effects to the growth and development of plant, it is biological that plant resistant fungi, bacterium and virus etc. can not only be enhanced
The ability of stress, but also plant is affected to heavy metal, ozone, ultraviolet radioactive, low temperature, heat shock, water deficit and salt damage etc.
The resistance of abiotic stress.
Currently, existing report is ground respectively with the drought resistance of plant, salt-resistance, winter resistance etc. about BR and SA mostly
Study carefully, there has been no BR and SA collective effect to alleviate cadmium to the report of the application of tobacco oxidative stress.
Summary of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide brassinosteroids and salicylic composition to alleviate
Application of the cadmium to tobacco oxidative stress.
Technical solution of the present invention is summarized as follows:
Brassinosteroid and salicylic composition alleviate application of the cadmium to tobacco oxidative stress, the brassinosteroid and
Salicylic composition is 1:(0.7-7.2 in mass ratio) ratio be made of brassinosteroid and salicylic acid.
Preferably, brassinosteroid and salicylic mass ratio are 1:2.2.
Advantages of the present invention:
(1) composition of the invention is pollution-free, does not injure to plant.
(2) of the invention cost is relatively low, experiments have shown that brassinosteroid and salicylic composition alleviate cadmium to tobacco oxygen
Change coercion.
Detailed description of the invention
Fig. 1 is tobacco H in varied situations2O2Content.
Fig. 2 is tobacco O in varied situations2 -Content.
Fig. 3 is tobacco mda content in varied situations.
In figure, WT is 1 wild-type tobacco of reference examples；WT+Cd is reference examples 2；WT+Cd+BR is reference examples 3；WT+Cd+SA
For reference examples 4；WT+Cd+BR+SA is embodiment 4.
Specific embodiment
The present invention by taking wild-type tobacco as an example, be in order to enable those skilled in the art to more fully understand the present invention,
But the present invention is not imposed any restrictions.
Wild-type tobacco seed, Nutrition Soil, vermiculite, perlite are commercially available.
CdCl2Reagent is purchased from Tianjin Yuan Li Chemical Co., Ltd., and purity is that analysis is pure.
BR reagent (brassinosteroid) is purchased from Tianjin Ding Guo biotech firm, and purity is that analysis is pure.
SA reagent (salicylic acid) is purchased from Tianjin Ding Guo biotech firm, purity 98%.
Polysorbas20 is purchased from Tianjin Ding Guo biotech firm.
The measurement of physical signs: H2O2Content, O2 -Content and mda content are measured by detection kit.
The present invention is further illustrated combined with specific embodiments below.
Embodiment 1
Brassinosteroid and salicylic composition, are made of brassinosteroid and salicylic acid.Brassinosteroid and bigcatkin willow
The mass ratio of acid is 1:2.2.
Embodiment 2
Brassinosteroid and salicylic composition, are made of brassinosteroid and salicylic acid.Brassinosteroid and bigcatkin willow
The mass ratio of acid is 1:0.7.
Embodiment 3
Brassinosteroid and salicylic composition, are made of brassinosteroid and salicylic acid.Brassinosteroid and bigcatkin willow
The mass ratio of acid is 1:7.2.
Embodiment 4
Brassinosteroid and salicylic composition alleviate application of the cadmium to tobacco oxidative stress, comprising the following steps:
(1) seed sprouting is carried out:
The wild-type tobacco seed of the full uniform maturation of picking, seed is seeded in equipped with Nutrition Soil, vermiculite and perlite
Plastic tub in, the volume ratio of three is 2:2:1,25 DEG C/22 DEG C round the clock, brightness 16h/8h cultivated in alternate greenhouse, to
Wild-type tobacco seed is sprouted, is grown；
(2) processing of tobacco seedling:
When tobacco seedling grows to 51 heart of leaf, form is chosen well with the consistent tobacco seedling of growing way, moves into caddy
Concentration is in the soil of 20mg/kg, and every 4d pours 1 clear water, and every 2d sprays the solution of the composition of 1 embodiment 1 (with ethyl alcohol
For solvent, the mother liquor of the final concentration of 1mg/mL of brassinosteroid, the final concentration of 2.2mg/mL of salicylic acid are prepared, in use, using water
It is diluted to the final concentration of 0.4mg/L of brassinosteroid, the final concentration of 0.88mg/L of salicylic acid), volume fraction is added when sprinkling is
0.2% polysorbas20 is uniformly sprayed as solvent, with sprayer in plant leaf tow sides, whole with blade tow sides
Wet and no liquid is dripped, abundant, uniform to guarantee to spray.
(3) physiological index determining:
After tobacco seedling handles 21d, the 2nd blade is taken from top to bottom, measures H2O2Content, O2 -Content and malonaldehyde contain
Amount.Reference examples 1:
Step (1) is the same as 4 step of embodiment (1)；
(2) processing of tobacco seedling:
When tobacco seedling grows to 51 heart of leaf, form is chosen well with the consistent tobacco seedling of growing way, moves into soil (no
Containing cadmium) in, every 4d pours 1 clear water, and every 2d sprays 1 distilled water, and when sprinkling is added the polysorbas20 that volume fraction is 0.2% and makees
It for solvent, is uniformly sprayed with sprayer in plant leaf tow sides, with blade tow sides, all wet and no liquid is dripped
Under, it is abundant, uniform to guarantee to spray.
Step (3) is the same as 4 step of embodiment (3).
Reference examples 2:
Step (1) is the same as 4 step of embodiment (1)；
(2) processing of tobacco seedling:
When tobacco seedling grows to 51 heart of leaf, form is chosen well with the consistent tobacco seedling of growing way, moves into caddy
Concentration is in the soil of 20mg/kg, and every 4d pours 1 clear water, and every 2d sprays 1 distilled water, and when sprinkling is added volume fraction and is
0.2% polysorbas20 is uniformly sprayed as solvent, with sprayer in plant leaf tow sides, whole with blade tow sides
Wet and no liquid is dripped, abundant, uniform to guarantee to spray.
Step (3) is the same as 4 step of embodiment (3).
Reference examples 3:
Step (1) is the same as 4 step of embodiment (1)；
(2) processing of tobacco seedling:
When tobacco seedling grows to 51 heart of leaf, form is chosen well with the consistent tobacco seedling of growing way, moves into caddy
Concentration is in the soil of 20mg/kg, and every 4d pours 1 clear water, and every 2d sprays the BR of 1 1.28mg/L, and volume is added in when sprinkling
The polysorbas20 that score is 0.2% is uniformly sprayed with sprayer in plant leaf tow sides, as solvent with blade positive and negative two
All wet and no liquid is dripped in face, abundant, uniform to guarantee to spray.
Step (3) is the same as 4 step of embodiment (3).
Reference examples 4:
Step (1) is the same as 1 step of embodiment (1)；
(2) processing of tobacco seedling:
When tobacco seedling grows to 51 heart of leaf, form is chosen well with the consistent tobacco seedling of growing way, moves into caddy
Concentration is in the soil of 20mg/kg, and every 4d pours 1 clear water, and every 2d sprays the SA of 1 1.28mg/L, and volume is added in when sprinkling
The polysorbas20 that score is 0.2% is uniformly sprayed with sprayer in plant leaf tow sides, as solvent with blade positive and negative two
All wet and no liquid is dripped in face, abundant, uniform to guarantee to spray.
Step (3) is the same as 4 step of embodiment (3).
Experimental result:
(1) obvious from can be seen that tobacco intracorporal active o content when being coerced by Cd in reference examples 2 in Fig. 1,2
It increases, compared with reference examples 2, the intracorporal active o content of reference examples 3,4 tobaccos is significantly reduced, the intracorporal work of 4 tobacco of embodiment
Property oxygen content further decrease, show that the composition (embodiment 1) of BR and SA can more efficiently remove the work generated in tobacco body
Property oxygen, to improve to the resistance of Cd.
(2) from the figure 3, it may be seen that the intracorporal mda content highest of 2 tobacco of reference examples, illustrates that Cd exacerbates tobacco cell adipose membrane
Extent of peroxidation, the intracorporal mda content of 4 tobacco of embodiment is minimum, shows that the composition of BR and SA can utmostly inhibit
Lipid peroxidation metabolism alleviates Cd to the oxidative stress of tobacco plant.
It is demonstrated experimentally that the composition of embodiment 2, embodiment 3 is to tobacco H2O2Content, O2 -The shadow of content and mda content
Sound is similar to the composition of embodiment 1.
Claims (2)
1. brassinosteroid and salicylic composition alleviate application of the cadmium to tobacco oxidative stress, it is characterized in that the rape is plain
Lactone and salicylic composition are 1:(0.7-7.2 in mass ratio) ratio be made of brassinosteroid and salicylic acid.
2. application according to claim 1, it is characterized in that brassinosteroid and salicylic mass ratio are 1:2.2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910614195.1A CN110338024A (en) | 2019-07-09 | 2019-07-09 | Brassinosteroid and salicylic composition alleviate application of the cadmium to tobacco oxidative stress |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910614195.1A CN110338024A (en) | 2019-07-09 | 2019-07-09 | Brassinosteroid and salicylic composition alleviate application of the cadmium to tobacco oxidative stress |
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| CN110338024A true CN110338024A (en) | 2019-10-18 |
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| CN201910614195.1A Pending CN110338024A (en) | 2019-07-09 | 2019-07-09 | Brassinosteroid and salicylic composition alleviate application of the cadmium to tobacco oxidative stress |
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Citations (9)
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|---|---|---|---|---|
| CN105191942A (en) * | 2015-09-30 | 2015-12-30 | 贵州省烟草科学研究院 | Preparation for reducing accumulation of chromium in tobacco and using method of preparation |
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| CN106171579A (en) * | 2016-07-18 | 2016-12-07 | 甘肃农业大学 | External source brassinosteroid processes the method alleviating the injury of corn seedling cadmium environment stress |
| CN107136085A (en) * | 2017-07-03 | 2017-09-08 | 中国计量大学 | Application of 24 Epibrassinolides in reduction Cadmium in Rice accumulation |
| CN107409800A (en) * | 2017-06-21 | 2017-12-01 | 四川农业大学 | Leaf lettuce cadmium content and the method for improving its quality are significantly reduced using salicylic acid |
| CN107517709A (en) * | 2017-09-14 | 2017-12-29 | 河南农业大学 | It is a kind of to prevent high temperature from forcing ripe planting method of tobacco |
| CN108401872A (en) * | 2018-03-30 | 2018-08-17 | 陕西科技大学 | It is a kind of to alleviate ciltivating process and salicylic application of the cadmium to tomato toxic action using salicylic acid |
| CN108794168A (en) * | 2018-06-27 | 2018-11-13 | 中国农业科学院农业资源与农业区划研究所 | It reduces tobacco leaf cadmium content and improves the foliage spraying agent and preparation method thereof of quality |
| CN109644723A (en) * | 2019-01-25 | 2019-04-19 | 四川大学 | A method of arabidopsis is improved to heavy metal cadmium tolerance |
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2019
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| PL2622081T3 (en) * | 2010-09-30 | 2016-09-30 | Tobacco with reduced cadmium content | |
| CN105191942A (en) * | 2015-09-30 | 2015-12-30 | 贵州省烟草科学研究院 | Preparation for reducing accumulation of chromium in tobacco and using method of preparation |
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| CN107409800A (en) * | 2017-06-21 | 2017-12-01 | 四川农业大学 | Leaf lettuce cadmium content and the method for improving its quality are significantly reduced using salicylic acid |
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| CN108401872A (en) * | 2018-03-30 | 2018-08-17 | 陕西科技大学 | It is a kind of to alleviate ciltivating process and salicylic application of the cadmium to tomato toxic action using salicylic acid |
| CN108794168A (en) * | 2018-06-27 | 2018-11-13 | 中国农业科学院农业资源与农业区划研究所 | It reduces tobacco leaf cadmium content and improves the foliage spraying agent and preparation method thereof of quality |
| CN109644723A (en) * | 2019-01-25 | 2019-04-19 | 四川大学 | A method of arabidopsis is improved to heavy metal cadmium tolerance |
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Application publication date: 20191018 |