CN107155672A - Method for adjusting and controlling rice heavy metal cadmium accumulation - Google Patents
Method for adjusting and controlling rice heavy metal cadmium accumulation Download PDFInfo
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- CN107155672A CN107155672A CN201710531489.9A CN201710531489A CN107155672A CN 107155672 A CN107155672 A CN 107155672A CN 201710531489 A CN201710531489 A CN 201710531489A CN 107155672 A CN107155672 A CN 107155672A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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Abstract
The invention provides a kind of method for adjusting and controlling rice heavy metal cadmium accumulation, it is related to Rice Cropping technical field, 24 Epibrassinolides and water are configured to the 24 Epibrassinolide solution that concentration is 150~300 μ g/L by the present invention, then spray 24 Epibrassinolide solution on the rice leaf of Cd stress culture during Rice Cropping.A kind of method for adjusting and controlling rice heavy metal cadmium accumulation, the either safety in production to paddy rice can be provided by the research of the present invention, or all there is highly beneficial realistic meaning to the reparation of cadmium pollution soil.Meanwhile, paddy rice is agriculture conventional method as conventional crop, its field management, reduces the operation difficulty that this method is further applied.
Description
Technical field
The present invention relates to Rice Cropping technical field, more particularly, to a kind of side for adjusting and controlling rice heavy metal cadmium accumulation
Method.
Background technology
Paddy rice is the indispensable crop of our human survivals, is also that we are main in one of edible grain, therefore water
The production safety and food sanitation standard of rice are closely bound up with us.It has been reported that, largely ploughed by heavy metal cadmium in China at this stage
Pollution, and it is related to national 11 provinces and cities, cause substantial amounts of grain drop in production and pollution, lose up to more than 20,000,000,000 yuan, be scope face
Product is big, loses huge.Meanwhile, pollution of heavy metals in rice is also different from other plant, and the cadmium accumulated in rice plant can then lead to
The food chain crossed in ecology enters human body, so as to endanger the physical and mental health of the mankind, it has powerful hidden and nature of danger.
It is very easy to absorb cadmium element after plantation in cadmium pollution soil because paddy rice has, is enriched with, and
Paddy rice normal growth feature is nor affected on while accumulating higher rapid determination of content of cadmium element, constantly has the news report whole nation each nearly ten years
Serious cadmium element Polluted Paddy event occurs for ground, and cadmium element severe overweight, contaminated matter are once reported if the ground such as Guangdong, Hunan Xiang River
Part, these events cause tremendous influence in ecological environment and mankind's activity, meanwhile, it is easy to be enriched with cadmium element using paddy rice
Characteristic, and then the research repaired to cadmium pollution soil also increasingly causes the attention of people.
Therefore, one kind is researched and developed on the basis of conventional rice plantation, is coerced using the administration of allogenic material, and then to cadmium
Compel the method that heavy metal cadmium accumulation of paddy is regulated and controled, the either safety in production to paddy rice, or repaiied to cadmium pollution soil
It is multiple that all there is highly beneficial realistic meaning.
In view of this, it is special to propose the present invention.
The content of the invention
The first object of the present invention is to provide a kind of method of adjusting and controlling rice heavy metal cadmium accumulation, and this method passes through in water
Seed rice sprays 24- Epibrassinolide solution on the rice leaf of Cd stress culture, and then to Cd stress water during planting
Rice heavy metal cadmium accumulation is regulated and controled, the application process have do not needed in low cost, simple to operate, application process it is special
Instrument and application cycle short advantage.
A kind of method for adjusting and controlling rice heavy metal cadmium accumulation that the present invention is provided, comprises the following steps：By 24- tables rape element
Lactones is configured to the 24- Epibrassinolide solution that concentration is 150~300 μ g/L with water, then will during Rice Cropping
24- Epibrassinolide solution is sprayed on the rice leaf of Cd stress culture.
Further, the concentration gradient of the 24- Epibrassinolides solution be 150 μ g/L, 180 μ g/L, 220 μ g/L,
280 μ g/L and 300 μ g/L.
Further, the water is distilled water.
Further, the Cd stress culture is to enter in the cadmium pollution soil that cadmium element concentration is 1.2~1.6mg/kg
Row plantation.
Further, the pH value of the cadmium pollution soil is 5~6.
Further, the time that the 24- Epibrassinolides solution is sprayed is rice seedling, tillering stage, heading early stage
With the 1st~14 day after blooming stage.
Further, the amount of spraying that the 24- Epibrassinolides solution is sprayed be every mu 300~350L/ days.
Further, the amount of spraying that the 24- Epibrassinolides solution is sprayed be every mu 330L/ days.
Further, the kind of the paddy rice is excellent No. 9 of river in Zhejiang Province.
Further, periodically paddy rice is watered during the Rice Cropping, the water content for keeping soil is 60~80%.
Compared with prior art, beneficial effects of the present invention are：
The invention provides a kind of new opplication method of 24- Epibrassinolides, it is by concentration during Rice Cropping
150~300 μ g/L 24- Epibrassinolide solution is sprayed on the rice leaf of Cd stress culture, can be to rice plant
Cd accumulation produces different effects.Research is found that the higher 24- Epibrassinolides of concentration are molten in concentration range of the present invention
Liquid can suppress the Cd accumulation in Cd stress paddy rice, and the relatively low 24- Epibrassinolides solution of concentration can promote Cd stress water
While Cd accumulation in rice stem, also there is inhibitory action to the Cd accumulation in Brown Rice, and then ensureing that Brown Rice reaches
To national food sanitation standard.A kind of method of adjusting and controlling rice heavy metal cadmium accumulation can be provided by the research of the present invention, either
Safety in production to paddy rice, or all there is highly beneficial realistic meaning to the reparation of cadmium pollution soil.Meanwhile, paddy rice conduct
Conventional crop, its field management is agriculture conventional method, reduces the operation difficulty that this method is further applied.
Embodiment
Technical scheme is clearly and completely described below in conjunction with embodiment, it is clear that described reality
It is a part of embodiment of the invention to apply example, rather than whole embodiments.Based on the embodiment in the present invention, the common skill in this area
The every other embodiment that art personnel are obtained under the premise of creative work is not made, belongs to the model that the present invention is protected
Enclose.
According to an aspect of the present invention, a kind of method of adjusting and controlling rice heavy metal cadmium accumulation, comprises the following steps：Will
24- Epibrassinolides are configured to the 24- Epibrassinolide solution that concentration is 150~300 μ g/L with water, then in rice seed
24- Epibrassinolide solution is sprayed on the rice leaf of Cd stress culture during plant.
Brassinosteroid also known as brassin lactones, be after five big plants hormone (auxin, the basic element of cell division, gibberellin,
Ethene, abscisic acid) after the sixth-largest class natural phytohormone, be widely present in each organ of plant, be acknowledged as activity
Highest, the plant hormone with wide spectrum, efficient physiological effect.Progress and the continuous progress of science and technology with the epoch, 20th century
Start the eighties to synthesize artificial high activity brassinosteroid analogs, and referred to as epi-brassinolide.24- epi-brassinolides
It is then to be formed by appropriate natural steroid compound (mainly ergosterol) for raw material transformation, with more preferable activity and warp
Ji property.
The invention provides a kind of new opplication method of 24- Epibrassinolides, it is by concentration during Rice Cropping
150~300 μ g/L 24- Epibrassinolide solution is sprayed on the rice leaf of Cd stress culture, can be to rice plant
Cd accumulation produces different effects.Research is obtained, the higher 24- Epibrassinolides of concentration are molten in concentration range of the present invention
Liquid can suppress the Cd accumulation in Cd stress paddy rice, and the relatively low 24- Epibrassinolides solution of concentration can promote Cd stress water
While Cd accumulation in rice stem, also there is inhibitory action to the Cd accumulation in Brown Rice, and then ensureing that Brown Rice reaches
To national food sanitation standard.A kind of method of adjusting and controlling rice heavy metal cadmium accumulation can be provided by the research of the present invention, either
Safety in production to paddy rice, or all there is highly beneficial realistic meaning to the reparation of cadmium pollution soil.Meanwhile, paddy rice conduct
Conventional crop, its field management is agriculture conventional method, reduces the operation difficulty that this method is further applied.
In the preferred embodiment of the present invention, the concentration gradient of the 24- Epibrassinolides solution is 150 μ
G/L, 180 μ g/L, 220 μ g/L, 280 μ g/L and 300 μ g/L.
In the preferred embodiment of the present invention, the water is distilled water.Distilled water is to make water using distillation equipment
Steam, then makes vapor congeal into water, and the distilled water prepared eliminates heavy metal ion.Present invention application distilled water is matched somebody with somebody
24- Epibrassinolides solution processed, can avoid influence of the heavy metal ion in water to experiment, the result of the experiment made is more
Accurately.
In the preferred embodiment of the present invention, the Cd stress culture be cadmium element concentration be 1.2~
Planted in 1.6mg/kg cadmium pollution soil.Content of the cadmium in natural soils is usually that performance is relatively low, will not be to the mankind
Very big harm is caused, it is 0.097mg/kg that the Cadmium in Soil background value of China, which is about,.The present invention is used for the soil of rice cultivation
It is 1.2~1.6mg/kg for cadmium concentration, is the soil of heavy metal cadmium severe contamination.
In the preferred embodiment of the present invention, the pH value of the cadmium pollution soil is 5~6.
It has been investigated that, the soil of different pH value is accumulated to the cadmium element of Cd stress paddy rice has adjustment effect, the present invention
Using pH value for 5~6 cadmium pollution soil carry out Rice Cropping, can exclude due to pH value difference formed by Cd stress
The influence of the cadmium element accumulation of paddy rice, and then preferably prove 24- Epibrassinolides in reduction Cadmium in Rice accumulation
Technique effect.
In the preferred embodiment of the present invention, the time that the 24- Epibrassinolides solution is sprayed is paddy rice
The 1st~14 day after seedling stage, tillering stage, heading early stage and blooming stage.
As a preferred embodiment, the present invention after rice seedling, tillering stage, heading early stage and blooming stage the
Rice leaf was carried out in 1~14 day spraying processing, the selection of such spraying time can make in the whole growth cycle of paddy rice,
There is external source 24- Epibrassinolides solution to carry out spraying effect.
In the preferred embodiment of the present invention, the amount of spraying that the 24- Epibrassinolides solution is sprayed is every
Mu 300~350L/ days.
In above-mentioned preferred embodiment, the amount of spraying that the 24- Epibrassinolides solution is sprayed is every mu of 330L/
My god.
It is preferred that, the amount of spraying for spraying processing adheres to full water pearl to spray to rice leaf, and liquid starts lower drop and is
Only.
In the preferred embodiment of the present invention, the kind of the paddy rice is excellent No. 9 of river in Zhejiang Province.
As a preferred embodiment, excellent No. 9 of river in Zhejiang Province is round-grained rice type series of three-series hybrid rice kind.Make single in the middle and lower reach of Yangtze River
Season late rice plantation, it has, and plant type is moderate, growing way is in great numbers, the ripe preferable advantage of phase annesl, but excellent No. 9 of river in Zhejiang Province is to the degeneration-resistant of Cd stress
Property it is not strong, belong to cadmium height accumulation rice varieties, the present invention using excellent No. 9 paddy rice in river in Zhejiang Province can preferably prove 24- tables rape element in
Technique effect of the fat in reduction Cadmium in Rice accumulation.
In the preferred embodiment of the present invention, periodically paddy rice is watered during the Rice Cropping, keeps soil
The water content of earth is 60~80%.
As a preferred embodiment, periodically being watered during Rice Cropping to paddy rice, the aqueous of soil is kept
Measuring can be such that paddy rice is preferably grown for 60~80%, it is to avoid other factors are to adjusting and controlling rice heavy metal cadmium accumulation of the present invention
The interference of research.
Technical scheme is described further below in conjunction with embodiment.
Following each embodiments are experiment kind with excellent No. 9 of main cultivated rice cultivars river in Zhejiang Province, Zhejiang Province, cadmium concentration be 1.3~
1.6mg/kg, the pH value of soil is plantation in 5~6 cadmium pollution soil.
In each following embodiment, Rice Cropping pattern is：Often handle 2.35 × 2.55m=6m of cell floor space2, product
Inter-species interval 0.4m, seeding row spacing 0.3m × 0.2m, are 125 clumps, per ridge building between cell and add plastic foil antiseepage, each handle into
3 repetitions of row.Unless otherwise specified, site preparation, nursery, transplanting, field management are agriculture conventional method.
Embodiment 1
First, 24- Epibrassinolides are configured to the aqueous solution that concentration is 150 μ g/L with distilled water, it is then dirty in cadmium
During the Rice Cropping for contaminating soil, using above-mentioned 24- Epibrassinolides solution in rice seedling, tillering stage, heading early stage
Continuously rice leaf is carried out with the 1st~14 day after blooming stage to spray processing, 24- Epibrassinolide solution in processing is sprayed
The amount of spraying for every mu 330L/ days.The whole planting process of paddy rice is carried out using conventional farming method.
Embodiment 2
First, 24- Epibrassinolides are configured to the aqueous solution that concentration is 180 μ g/L with distilled water, it is then dirty in cadmium
During the Rice Cropping for contaminating soil, using above-mentioned 24- Epibrassinolides solution in rice seedling, tillering stage, heading early stage
Continuously rice leaf is carried out with the 1st~14 day after blooming stage to spray processing, 24- Epibrassinolide solution in processing is sprayed
The amount of spraying for every mu 330L/ days.The whole planting process of paddy rice is carried out using conventional farming method.
Embodiment 3
First, 24- Epibrassinolides are configured to the aqueous solution that concentration is 220 μ g/L with distilled water, it is then dirty in cadmium
During the Rice Cropping for contaminating soil, using above-mentioned 24- Epibrassinolides solution in rice seedling, tillering stage, heading early stage
Continuously rice leaf is carried out with the 1st~14 day after blooming stage to spray processing, 24- Epibrassinolide solution in processing is sprayed
The amount of spraying for every mu 330L/ days.The whole planting process of paddy rice is carried out using conventional farming method.
Embodiment 4
First, 24- Epibrassinolides are configured to the aqueous solution that concentration is 280 μ g/L with distilled water, it is then dirty in cadmium
During the Rice Cropping for contaminating soil, using above-mentioned 24- Epibrassinolides solution in rice seedling, tillering stage, heading early stage
Continuously rice leaf is carried out with the 1st~14 day after blooming stage to spray processing, 24- Epibrassinolide solution in processing is sprayed
The amount of spraying for every mu 330L/ days.The whole planting process of paddy rice is carried out using conventional farming method.
Embodiment 5
First, 24- Epibrassinolides are configured to the aqueous solution that concentration is 300mg/L with distilled water, it is then dirty in cadmium
During the Rice Cropping for contaminating soil, using above-mentioned 24- Epibrassinolides solution in rice seedling, tillering stage, heading early stage
Continuously rice leaf is carried out with the 1st~14 day after blooming stage to spray processing, 24- Epibrassinolide solution in processing is sprayed
The amount of spraying for every mu 330L/ days.The whole planting process of paddy rice is carried out using conventional farming method.
Comparative example 1
During the Rice Cropping of cadmium pollution soil, using distilled water is in rice seedling, tillering stage, heading early stage and raises
Continuously rice leaf is carried out to spray processing within the 1st~14 day after florescence, the amount of spraying for spraying distilled water in processing is every mu
330L/ days.The whole planting process of paddy rice is carried out using conventional farming method.
Comparative example 2
First, 24- Epibrassinolides are configured to the aqueous solution that concentration is 100mg/L with distilled water, it is then dirty in cadmium
During the Rice Cropping for contaminating soil, using above-mentioned 24- Epibrassinolides solution in rice seedling, tillering stage, heading early stage
Continuously rice leaf is carried out with the 1st~14 day after blooming stage to spray processing, 24- Epibrassinolide solution in processing is sprayed
The amount of spraying for every mu 330L/ days.The whole planting process of paddy rice is carried out using conventional farming method.
Comparative example 3
First, 24- Epibrassinolides are configured to the aqueous solution that concentration is 350mg/L with distilled water, it is then dirty in cadmium
During the Rice Cropping for contaminating soil, using above-mentioned 24- Epibrassinolides solution in rice seedling, tillering stage, heading early stage
Continuously rice leaf is carried out with the 1st~14 day after blooming stage to spray processing, 24- Epibrassinolide solution in processing is sprayed
The amount of spraying for every mu 330L/ days.The whole planting process of paddy rice is carried out using conventional farming method.
The Agronomic Traits in Rice of effect example 1 is analyzed
The paddy rice of embodiment 1~5 and the plant of 1~3 kind of comparative example is gathered into Plant samples before cutting, and investigates do not exist together respectively
The agronomic shapes such as plant height, spike number, grain number per spike, mass of 1000 kernel and the setting percentage of Rice under Condition are managed, its result is as shown in the table：
As shown in upper table, concentration is carried out for 150~300 μ g/L 24- Epibrassinolides solution to rice leaf
Processing is sprayed, little is influenceed on the overall economical character of rice maturity.The control group sprayed with comparative example 1 using distilled water
Compare, under the 24- Epibrassinolides solution processing of the embodiment of the present invention 1~5 and comparative example 2 and comparative example 3, paddy rice strain
Height, spike number, grain number per spike, mass of 1000 kernel and setting percentage are all without significant changes, and paddy rice knot fringe, growth situation is good, is not affected by very big shadow
Ring.Therefore, concentration does not have very for 150~300 μ g/L 24- Epibrassinolides solution to the overall economical character of rice plant
Big influence, i.e. concentration do not have bad influence, 24- to rice plant for 150~300 μ g/L 24- Epibrassinolides solution
The effect of Epibrassinolide adjusting and controlling rice heavy metal cadmium accumulation is not due to obtained from the growth of regulation rice plant.
The regulation and control of the heavy metal cadmium accumulation for the brown rice that effect example 2 is obtained to Cd stress rice cultivation
By the paddy rice ripe and harvested of 1~3 kind of plant of embodiment 1~5 and comparative example after 60~70 DEG C of drying to constant weight, by water
Rice seed is shelled with hulling machine, obtains brown rice；Then the cadmium content of brown rice of the application conventional methods to obtaining is measured,
Its measurement result is as shown in the table：
As seen from the above table, the concentration of the embodiment of the present invention 1~5 is molten for 150~300 μ g/L 24- Epibrassinolides
The cadmium content that liquid to rice leaf spray after processing in Brown Rice is below heavy metal cadmium content in China's regulation brown rice
Maximum limit standard be 0.2mg/kg；Compared with the control group that comparative example 1 is sprayed using distilled water, the embodiment of the present invention
Cadmium content in 1~5 Brown Rice is significantly lower than control group.
Meanwhile, from above-mentioned data, concentration in concentration range of the present invention is used in the embodiment of the present invention 4 and embodiment 5
Higher 24- Epibrassinolides solution can substantially suppress the Cd accumulation in the brown rice of Cd stress paddy rice, make in cadmium pollution soil
The cadmium content for the brown rice planted in ground is reduced to below 0.13mg/kg, far below heavy metal cadmium content in China's regulation brown rice
Maximum limit standard be 0.2mg/kg.And the embodiment of the present invention 1~3 is with the reduction of 24- Epibrassinolide solution concentrations,
The cadmium content in Brown Rice after its processing also increases accordingly, thus, it can be known that as 24- Epibrassinolides of the present invention are molten
The reduction of concentration in liquid concentration range, the present invention successively decreases successively to the inhibition of the Cd accumulation in the brown rice of paddy rice.
By using the 24- Epibrassinolide solution beyond concentration range of the present invention to water with comparative example 2 and comparative example 3
The progress of rice blade face sprays the result obtained after processing and compared, as seen from the above table, and the cadmium content of the brown rice of 2 kinds of plants of comparative example is
0.22mg/kg, the cadmium content of the brown rice of 3 kinds of plants of comparative example is 0.20mg/kg, and its effect is carried out with comparative example 1 using distilled water
The control group of sprinkling is not obvious compared to gap.Therefore, using the 24- Epibrassinolide solution pair outside concentration range of the present invention
Rice leaf, which spray handling, can not reach the technique effect of the cadmium content of brown rice in adjusting and controlling rice plant of the present invention.
Regulation and control of the effect example 3 to the heavy metal cadmium accumulation of the base of the plant of Cd stress rice cultivation
By the paddy rice ripe and harvested of 1~3 kind of plant of embodiment 1~5 and comparative example after 60~70 DEG C of drying to constant weight, by water
The stem of rice plants is gathered in；Then application conventional methods are surveyed to the cadmium content of the stem of obtained rice plant
Fixed, its measurement result is as shown in the table：
As seen from the above table, compared with the control group that comparative example 1 is sprayed using distilled water, the embodiment of the present invention 5 is used
Concentration sprays the cadmium content of paddy rice stem after processing only for 300mg/L 24- Epibrassinolides solution to rice leaf progress
For 0.102mg/kg；The concentration of the embodiment of the present invention 4 is entered for 280 μ g/L 24- Epibrassinolide solution to rice leaf
The cadmium content that row sprays paddy rice stem after processing is 0.115mg/kg, it follows that concentration is higher in concentration range of the present invention
24- Epibrassinolides solution can substantially suppress Cd stress paddy rice paddy rice stem Cd accumulation.
By entering with the concentration of the embodiment of the present invention 2 for 180 μ g/L 24- Epibrassinolide solution to rice leaf
Row sprays the cadmium content up to 1.75mg/kg of paddy rice stem after processing；The concentration of embodiment 5 is 150 μ g/L 24- table rapes
Lipoprotein solution to rice leaf spray the cadmium content up to 1.86mg/kg of paddy rice stem after processing, the He of above-described embodiment 1 in plain
2 compared with the control group that comparative example 1 is sprayed using distilled water, it is known that the relatively low 24- of concentration in concentration range of the present invention
Epibrassinolide solution can be obviously promoted the Cd accumulation of Cd stress paddy rice stem.
By using the 24- Epibrassinolide solution beyond concentration range of the present invention to water with comparative example 2 and comparative example 3
The progress of rice blade face sprays the result obtained after processing and compared, as seen from the above table, and the cadmium content of the brown rice of 2 kinds of plants of comparative example is
1.21mg/kg, the cadmium content of the brown rice of 3 kinds of plants of comparative example is 1.23mg/kg, and its effect is carried out with comparative example 1 using distilled water
The control group of sprinkling is not obvious compared to gap.Therefore, using the 24- Epibrassinolide solution pair outside concentration range of the present invention
Rice leaf, which spray handling, can not reach the technique effect of paddy rice stem cadmium content in adjusting and controlling rice plant of the present invention.
In summary, the present invention during Rice Cropping by concentration be 100~200mg/L 24- Epibrassinolides
Solution is sprayed on the rice leaf of Cd stress culture, can produce different effects to the Cd accumulation of rice plant.Through above-mentioned effect
Fruit example 2 and effect example 3 understand that the higher 24- Epibrassinolides solution of concentration can suppress cadmium in concentration range of the present invention
The Cd accumulation in paddy rice is coerced, the relatively low 24- Epibrassinolides solution of concentration can promote the cadmium in Cd stress paddy rice stem
While accumulation, also there is inhibitory action to the Cd accumulation in Brown Rice, and then ensureing that Brown Rice reaches the edible mark of country
It is accurate.A kind of method of adjusting and controlling rice heavy metal cadmium accumulation, the either safety to paddy rice can be provided by the research of the present invention
Production, or all there is highly beneficial realistic meaning to the reparation of cadmium pollution soil.Meanwhile, paddy rice as conventional crop, its
Field management is agriculture conventional method, reduces the operation difficulty that this method is further applied.
Finally it should be noted that：Various embodiments above is merely illustrative of the technical solution of the present invention, rather than its limitations；To the greatest extent
The present invention is described in detail with reference to foregoing embodiments for pipe, it will be understood by those within the art that：Its according to
The technical scheme described in foregoing embodiments can so be modified, or which part or all technical characteristic are entered
Row equivalent substitution；And these modifications or replacement, the essence of appropriate technical solution is departed from various embodiments of the present invention technology
The scope of scheme.
Claims (10)
1. a kind of method for adjusting and controlling rice heavy metal cadmium accumulation, it is characterised in that comprise the following steps：By 24- table rapes
Plain lactones is configured to the 24- Epibrassinolide solution that concentration is 150~300 μ g/L with water, then during Rice Cropping
24- Epibrassinolide solution is sprayed on the rice leaf of Cd stress culture.
2. according to the method described in claim 1, it is characterised in that the concentration gradient of the 24- Epibrassinolides solution is
150 μ g/L, 180 μ g/L, 220 μ g/L, 280 μ g/L and 300 μ g/L.
3. according to the method described in claim 1, it is characterised in that the water is distilled water.
4. according to the method described in claim 1, it is characterised in that the Cd stress culture be cadmium element concentration be 1.2~
Planted in 1.6mg/kg cadmium pollution soil.
5. method according to claim 4, it is characterised in that the pH value of the cadmium pollution soil is 5~6.
6. according to the method described in claim 1, it is characterised in that the time that the 24- Epibrassinolides solution is sprayed is
The 1st~14 day after rice seedling, tillering stage, heading early stage and blooming stage.
7. method according to claim 6, it is characterised in that the amount of spraying that the 24- Epibrassinolides solution is sprayed
For every mu 300~350L/ days.
8. method according to claim 7, it is characterised in that the amount of spraying that the 24- Epibrassinolides solution is sprayed
For every mu 330L/ days.
9. according to the method described in claim 1, it is characterised in that the kind of the paddy rice is excellent No. 9 of river in Zhejiang Province.
10. the method according to any one of claim 1~9, it is characterised in that periodically to water during the Rice Cropping
Rice is watered, and the water content for keeping soil is 60~80%.
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| CN106171579A (en) * | 2016-07-18 | 2016-12-07 | 甘肃农业大学 | External source brassinosteroid processes the method alleviating the injury of corn seedling cadmium environment stress |
| CN106583431A (en) * | 2016-12-21 | 2017-04-26 | 河南科技学院 | Bioremediation method for soil polluted by heavy metal lead cadmium and base on kosteletzkya virginica |
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2017
- 2017-07-03 CN CN201710531489.9A patent/CN107155672A/en not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105191942A (en) * | 2015-09-30 | 2015-12-30 | 贵州省烟草科学研究院 | Preparation for reducing accumulation of chromium in tobacco and using method of preparation |
| CN106171579A (en) * | 2016-07-18 | 2016-12-07 | 甘肃农业大学 | External source brassinosteroid processes the method alleviating the injury of corn seedling cadmium environment stress |
| CN106583431A (en) * | 2016-12-21 | 2017-04-26 | 河南科技学院 | Bioremediation method for soil polluted by heavy metal lead cadmium and base on kosteletzkya virginica |
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