CN107616068A - A kind of high efficiency, low cost potato virus-free plantlet and seed production technology - Google Patents

A kind of high efficiency, low cost potato virus-free plantlet and seed production technology Download PDF

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Publication number
CN107616068A
CN107616068A CN201711006800.4A CN201711006800A CN107616068A CN 107616068 A CN107616068 A CN 107616068A CN 201711006800 A CN201711006800 A CN 201711006800A CN 107616068 A CN107616068 A CN 107616068A
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China
Prior art keywords
soil
seedling
seedbed
detoxic seedling
detoxic
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CN201711006800.4A
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Chinese (zh)
Inventor
饶永峰
谌红燕
吴光美
曾祥盛
陈明艳
陈晓
陈洪滔
任明国
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Guizhou Agricultural Science And Technology Co Ltd
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Guizhou Agricultural Science And Technology Co Ltd
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Priority to CN201711006800.4A priority Critical patent/CN107616068A/en
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Abstract

The present invention proposes a kind of high efficiency, low cost potato virus-free plantlet and seed production technology, comprises the following steps:Culturing room sterilizes, sterilizing is carried out to planting shed using disinfection sanitizer, matrix soil configures, solanaceous crops were not planted in selection soil soil in 3 years is collected, and dry sterilization 23 hours at a temperature of 70 90 DEG C, soil, coarse sand, farm manure, stalk plant ash, phosphate fertilizer, nitrogenous fertilizer are mixed in proportion even, mixed soil is moved on in the seedbed inside planting shed, then thin rake of deep ploughing is carried out to mixed soil, depth of soil is 8 12 centimetres, and disinfection sanitizer is sprayed, nursery bed disinfection.The high efficiency, low cost potato virus-free plantlet and seed production technology, the planting environment of potato can effectively be changed by the configuration of matrix soil, so as to reduce cost, pass through the implantation methods of refinement, rapidly and efficiently culture can be carried out to detoxic seedling to plant, additionally it is possible to effectively improve the production efficiency of potato.

Description

A kind of high efficiency, low cost potato virus-free plantlet and seed production technology
Technical field
The present invention relates to potato planting technical field, specially a kind of high efficiency, low cost potato virus-free plantlet and original seed life Production technology.
Background technology
Potato is annual herb plant, nickname potato, potato, potato etc., can chase after earliest the artificial cultivation of potato To trace back to the south of Peru area of about B.C. 8000 to 5000, potato is high 15-80 centimetres, hairless or by thin pubescence, Stem divides the aerial stem of plant and subterranean stem two parts, and potato is one of Chinese five big staple foods, and its nutritive value is high, adaptive faculty is strong, yield Greatly, it is the third-largest important cereal crops in the whole world, is only second to wheat and corn, potato is stem tuber breeding, can be used as medicine, mild-natured taste It is sweet, it can treat for stomachache, the disease such as Zha ribs, carbuncle swells, as food, its retention cycle is unsuitable oversize, must low temperature, drying, close Preservation is closed, the young shoot of potato has mild toxicity, and existing detoxic seedling needs cost high in incubation, and efficiency is low, plantation Method is coarse, and the yield of potato can be caused to decline.
The content of the invention
The technical problem to be solved in the present invention is to overcome the defects of existing, there is provided a kind of high efficiency, low cost potato virus-free plantlet And seed production technology, the planting environment of potato can effectively be changed by the configuration of matrix soil, so as to reduce cost, passed through The implantation methods of refinement, rapidly and efficiently culture can be carried out to detoxic seedling and is planted, additionally it is possible to effectively improves the production effect of potato Rate, effectively can solve the problems, such as in background technology.
To achieve the above object, the present invention provides a kind of high efficiency, low cost potato virus-free plantlet and seed production technology, bag Include following steps:
1)Culturing room sterilizes:Sterilizing is carried out to planting shed using disinfection sanitizer;
2)Matrix soil configures:Solanaceous crops were not planted in selection soil soil in 3 years is collected, and in 70-90 DEG C of temperature Lower dry sterilization 2-3 hours are spent, soil, coarse sand, farm manure, stalk plant ash, phosphate fertilizer, nitrogenous fertilizer are mixed in proportion even, will be mixed Soil after conjunction is moved on in the seedbed inside planting shed, and thin rake of deep ploughing then is carried out to mixed soil, and depth of soil is 8-12 lis Rice, and spray disinfection sanitizer;
3)Nursery bed disinfection:Transplanting seedbed is killed virus using disinfectant, and the transplanting seedbed after sterilization covered with film Lid sealing 2-4 days, and remove film and stand 3 days;
4)Detoxic seedling cleans:Detoxic seedling seedling is taken out from culture medium, and the liquid medium of the root of detoxic seedling seedling is used Clear water is clean clearly;
5)Detoxification transplantation of seedlings:Detoxic seedling after cleaning is transplanted on seedbed, and transplants detoxic seedling before first with clear water to seedling Bed is poured into a mould;
6)Detoxic seedling applies fertilizer:A small amount of chemical nitrogen fertilizer is added to apply fertilizer the detoxic seedling after transplanting with cleaning up excrement water;
7)Nurse in seedbed:After planting detoxic seedling seedling, seedbed is covered using film, film is removed after 6-8 days;
8)Later stage nurses:Intensity of illumination in planting shed, humidity and temperature are controlled, and in detoxic seedling different growth period The different nutrient solution of spray concentration;
9)Disease management:Sprinkling was carried out to seedbed every 3-4 days using Bordeaux mixture or metalaxyl to prevent;
10)Receipts are adopted:Harvested in detoxic seedling physiological maturity using artificial, overground part cauline leafs, and luck are removed in 6-8 days before harvest Go out planting shed, pay attention to reducing epidermis injury during harvest.
As a preferred technical solution of the present invention:In step 2, the soil raw material of mixing includes by weight:Soil 160-200 parts, coarse sand 100-120 parts, farm manure 40-60 parts, stalk plant ash 40-60 parts, 12-15 points of phosphate fertilizer, nitrogenous fertilizer 12-15 Part.
As a preferred technical solution of the present invention:In step 6, the weight ratio of cleaning up excrement water and chemical nitrogen fertilizer is 30-40: 1-4。
As a preferred technical solution of the present invention:In step 5, when being transplanted to detoxic seedling, adjacent two plants de- The horizontal spacing of malicious seedling is 6-8 centimetres, 8-10 centimetres of longitudinal pitch.
As a preferred technical solution of the present invention:In step 8, when carrying out later stage nursing to detoxic seedling, planting shed Interior temperature is maintained at 16-24 DEG C, and humidity is maintained at 60%-70%, and pays attention to the ventilation of planting shed.
Compared with prior art, the beneficial effects of the invention are as follows:Potato can effectively be changed by the configuration of matrix soil Planting environment, so as to reduce cost, by the implantation methods of refinement, rapidly and efficiently culture can be carried out to detoxic seedling and is planted, The production efficiency of potato can also be effectively improved.
Brief description of the drawings
Fig. 1 is a kind of high efficiency, low cost potato virus-free plantlet of the present invention and the flow chart of seed production technology.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete Site preparation describes, it is clear that described embodiment is only part of the embodiment of the present invention, rather than whole embodiments.It is based on Embodiment in the present invention, those of ordinary skill in the art are obtained every other under the premise of creative work is not made Embodiment, belong to the scope of protection of the invention.
Referring to Fig. 1, the present invention provides following technical scheme:
Embodiment one:A kind of high efficiency, low cost potato virus-free plantlet and seed production technology, comprise the following steps:
1)Culturing room sterilizes:Sterilizing is carried out to planting shed using disinfection sanitizer;
2)Matrix soil configures:Solanaceous crops were not planted in selection soil soil in 3 years is collected, and in 70 DEG C of temperature Lower dry sterilization 2 hours, soil, coarse sand, farm manure, stalk plant ash, phosphate fertilizer, nitrogenous fertilizer are mixed in proportion even, after mixing Soil move on in the seedbed inside planting shed, then carry out thin rake of deep ploughing to mixed soil, depth of soil is 8 centimetres, and is sprayed Disinfection sanitizer is spilt, and the soil raw material mixed includes by weight:160 parts of soil, 100 parts of coarse sand, 40 parts of farm manure, stalk 40 parts of plant ash, phosphate fertilizer 12 divides, 12 parts of nitrogenous fertilizer;
3)Nursery bed disinfection:Transplanting seedbed is killed virus using disinfectant, and the transplanting seedbed after sterilization covered with film Lid sealing 2 days, and remove film and stand 3 days;
4)Detoxic seedling cleans:Detoxic seedling seedling is taken out from culture medium, and the liquid medium of the root of detoxic seedling seedling is used Clear water is clean clearly;
5)Detoxification transplantation of seedlings:Detoxic seedling after cleaning is transplanted on seedbed, and transplants detoxic seedling before first with clear water to seedling Bed is poured into a mould, and the horizontal spacing of adjacent two plants of detoxic seedlings is 6 centimetres, 8 centimetres of longitudinal pitch;
6)Detoxic seedling applies fertilizer:A small amount of chemical nitrogen fertilizer is added to apply fertilizer the detoxic seedling after transplanting with cleaning up excrement water, cleaning up excrement water and chemical nitrogen fertilizer Weight ratio be 30:1;
7)Nurse in seedbed:After planting detoxic seedling seedling, seedbed is covered using film, film is removed after 6 days;
8)Later stage nurses:Intensity of illumination in planting shed, humidity and temperature are controlled, and in detoxic seedling different growth period The different nutrient solution of spray concentration, the temperature in planting shed are maintained at 16 DEG C, and humidity is maintained at 60%, and pays attention to planting shed Ventilation;
9)Disease management:Sprinkling was carried out to seedbed every 3 days using Bordeaux mixture or metalaxyl to prevent;
10)Receipts are adopted:Harvested in detoxic seedling physiological maturity using artificial, overground part cauline leafs are removed in 6 days before harvest, transport in time Planting shed, pay attention to during harvest reducing epidermis injury.
Embodiment two:A kind of high efficiency, low cost potato virus-free plantlet and seed production technology, comprise the following steps:
1)Culturing room sterilizes:Sterilizing is carried out to planting shed using disinfection sanitizer;
2)Matrix soil configures:Solanaceous crops were not planted in selection soil soil in 3 years is collected, and in 80 DEG C of temperature Lower dry sterilization 2 hours, soil, coarse sand, farm manure, stalk plant ash, phosphate fertilizer, nitrogenous fertilizer are mixed in proportion even, after mixing Soil move on in the seedbed inside planting shed, then carry out thin rake of deep ploughing to mixed soil, depth of soil is 9 centimetres, and is sprayed Disinfection sanitizer is spilt, and the soil raw material mixed includes by weight:175 parts of soil, 107 parts of coarse sand, farm manure, 46 parts, straw 46 parts of stalk plant ash, phosphate fertilizer, 13 points, 13 parts of nitrogenous fertilizer;
3)Nursery bed disinfection:Transplanting seedbed is killed virus using disinfectant, and the transplanting seedbed after sterilization covered with film Lid sealing 3 days, and remove film and stand 3 days;
4)Detoxic seedling cleans:Detoxic seedling seedling is taken out from culture medium, and the liquid medium of the root of detoxic seedling seedling is used Clear water is clean clearly;
5)Detoxification transplantation of seedlings:Detoxic seedling after cleaning is transplanted on seedbed, and transplants detoxic seedling before first with clear water to seedling Bed is poured into a mould, and the horizontal spacing of adjacent two plants of detoxic seedlings is 7 centimetres, 9 centimetres of longitudinal pitch;
6)Detoxic seedling applies fertilizer:A small amount of chemical nitrogen fertilizer is added to apply fertilizer the detoxic seedling after transplanting with cleaning up excrement water, cleaning up excrement water and chemical nitrogen fertilizer Weight ratio be 33:2;
7)Nurse in seedbed:After planting detoxic seedling seedling, seedbed is covered using film, film is removed after 7 days;
8)Later stage nurses:Intensity of illumination in planting shed, humidity and temperature are controlled, and in detoxic seedling different growth period The different nutrient solution of spray concentration, the temperature in planting shed are maintained at 18 DEG C, and humidity is maintained at 63%, and pays attention to planting shed Ventilation;
9)Disease management:Sprinkling was carried out to seedbed every 3 days using Bordeaux mixture or metalaxyl to prevent;
10)Receipts are adopted:Harvested in detoxic seedling physiological maturity using artificial, overground part cauline leafs are removed in 7 days before harvest, transport in time Planting shed, pay attention to during harvest reducing epidermis injury.
Embodiment three:A kind of high efficiency, low cost potato virus-free plantlet and seed production technology, comprise the following steps:
1)Culturing room sterilizes:Sterilizing is carried out to planting shed using disinfection sanitizer;
2)Matrix soil configures:Solanaceous crops were not planted in selection soil soil in 3 years is collected, and in 80 DEG C of temperature Lower dry sterilization 3 hours, soil, coarse sand, farm manure, stalk plant ash, phosphate fertilizer, nitrogenous fertilizer are mixed in proportion even, after mixing Soil move on in the seedbed inside planting shed, then carry out thin rake of deep ploughing to mixed soil, depth of soil is 11 centimetres, and is sprayed Disinfection sanitizer is spilt, and the soil raw material mixed includes by weight:185 parts of soil, 113 parts of coarse sand, 53 parts of farm manure, stalk Plant ash, 53 parts, phosphate fertilizer 14 divides, 14 parts of nitrogenous fertilizer;
3)Nursery bed disinfection:Transplanting seedbed is killed virus using disinfectant, and the transplanting seedbed after sterilization covered with film Lid sealing 3 days, and remove film and stand 3 days;
4)Detoxic seedling cleans:Detoxic seedling seedling is taken out from culture medium, and the liquid medium of the root of detoxic seedling seedling is used Clear water is clean clearly;
5)Detoxification transplantation of seedlings:Detoxic seedling after cleaning is transplanted on seedbed, and transplants detoxic seedling before first with clear water to seedling Bed is poured into a mould, and the horizontal spacing of adjacent two plants of detoxic seedlings is 7 centimetres, 9 centimetres of longitudinal pitch;
6)Detoxic seedling applies fertilizer:A small amount of chemical nitrogen fertilizer is added to apply fertilizer the detoxic seedling after transplanting with cleaning up excrement water, cleaning up excrement water and chemical nitrogen fertilizer Weight ratio be 36:3;
7)Nurse in seedbed:After planting detoxic seedling seedling, seedbed is covered using film, film is removed after 7 days;
8)Later stage nurses:Intensity of illumination in planting shed, humidity and temperature are controlled, and in detoxic seedling different growth period The different nutrient solution of spray concentration, the temperature in planting shed are maintained at 21 DEG C, and humidity is maintained at 66%, and pays attention to planting shed Ventilation;
9)Disease management:Sprinkling was carried out to seedbed every 4 days using Bordeaux mixture or metalaxyl to prevent;
10)Receipts are adopted:Harvested in detoxic seedling physiological maturity using artificial, overground part cauline leafs are removed in 7 days before harvest, transport in time Planting shed, pay attention to during harvest reducing epidermis injury.
Example IV:A kind of high efficiency, low cost potato virus-free plantlet and seed production technology, comprise the following steps:
1)Culturing room sterilizes:Sterilizing is carried out to planting shed using disinfection sanitizer;
2)Matrix soil configures:Solanaceous crops were not planted in selection soil soil in 3 years is collected, and in 90 DEG C of temperature Lower dry sterilization 3 hours, soil, coarse sand, farm manure, stalk plant ash, phosphate fertilizer, nitrogenous fertilizer are mixed in proportion even, after mixing Soil move on in the seedbed inside planting shed, then carry out thin rake of deep ploughing to mixed soil, depth of soil is 12 centimetres, and is sprayed Disinfection sanitizer is spilt, and the soil raw material mixed includes by weight:200 parts of soil, 120 parts of coarse sand, 60 parts of farm manure, stalk 60 parts of plant ash, phosphate fertilizer 15 divides, 15 parts of nitrogenous fertilizer;
3)Nursery bed disinfection:Transplanting seedbed is killed virus using disinfectant, and the transplanting seedbed after sterilization covered with film Lid sealing 4 days, and remove film and stand 3 days;
4)Detoxic seedling cleans:Detoxic seedling seedling is taken out from culture medium, and the liquid medium of the root of detoxic seedling seedling is used Clear water is clean clearly;
5)Detoxification transplantation of seedlings:Detoxic seedling after cleaning is transplanted on seedbed, and transplants detoxic seedling before first with clear water to seedling Bed is poured into a mould, and the horizontal spacing of adjacent two plants of detoxic seedlings is 8 centimetres, 10 centimetres of longitudinal pitch;
6)Detoxic seedling applies fertilizer:A small amount of chemical nitrogen fertilizer is added to apply fertilizer the detoxic seedling after transplanting with cleaning up excrement water, cleaning up excrement water and chemical nitrogen fertilizer Weight ratio be 40:4;
7)Nurse in seedbed:After planting detoxic seedling seedling, seedbed is covered using film, film is removed after 8 days;
8)Later stage nurses:Intensity of illumination in planting shed, humidity and temperature are controlled, and in detoxic seedling different growth period The different nutrient solution of spray concentration, the temperature in planting shed are maintained at 24 DEG C, and humidity is maintained at 70%, and pays attention to planting shed Ventilation;
9)Disease management:Every it is anti-sprinkling to be carried out to seedbed using Bordeaux mixture or metalaxyl within 4 days;
10)Receipts are adopted:Harvested in detoxic seedling physiological maturity using artificial, before harvest, remove within 8 days overground part cauline leaf, and luck Go out planting shed, pay attention to reducing epidermis injury during harvest.
Benefit of the present invention:The planting environment of potato can effectively be changed by the configuration of matrix soil, so as to reduce cost, By the implantation methods of refinement, rapidly and efficiently culture can be carried out to detoxic seedling and is planted, additionally it is possible to effectively improves the life of potato Produce efficiency.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with A variety of changes, modification can be carried out to these embodiments, replace without departing from the principles and spirit of the present invention by understanding And modification, the scope of the present invention is defined by the appended.

Claims (5)

1. a kind of high efficiency, low cost potato virus-free plantlet and seed production technology, it is characterised in that:Comprise the following steps:
1)Culturing room sterilizes:Sterilizing is carried out to planting shed using disinfection sanitizer;
2)Matrix soil configures:Solanaceous crops were not planted in selection soil soil in 3 years is collected, and in 70-90 DEG C of temperature Lower dry sterilization 2-3 hours are spent, soil, coarse sand, farm manure, stalk plant ash, phosphate fertilizer, nitrogenous fertilizer are mixed in proportion even, will be mixed Soil after conjunction is moved on in the seedbed inside planting shed, and thin rake of deep ploughing then is carried out to mixed soil, and depth of soil is 8-12 lis Rice, and spray disinfection sanitizer;
3)Nursery bed disinfection:Transplanting seedbed is killed virus using disinfectant, and the transplanting seedbed after sterilization covered with film Lid sealing 2-4 days, and remove film and stand 3 days;
4)Detoxic seedling cleans:Detoxic seedling seedling is taken out from culture medium, and the liquid medium of the root of detoxic seedling seedling is used Clear water is clean clearly;
5)Detoxification transplantation of seedlings:Detoxic seedling after cleaning is transplanted on seedbed, and transplants detoxic seedling before first with clear water to seedling Bed is poured into a mould;
6)Detoxic seedling applies fertilizer:A small amount of chemical nitrogen fertilizer is added to apply fertilizer the detoxic seedling after transplanting with cleaning up excrement water;
7)Nurse in seedbed:After planting detoxic seedling seedling, seedbed is covered using film, film is removed after 6-8 days;
8)Later stage nurses:Intensity of illumination in planting shed, humidity and temperature are controlled, and in detoxic seedling different growth period The different nutrient solution of spray concentration;
9)Disease management:Sprinkling was carried out to seedbed every 3-4 days using Bordeaux mixture or metalaxyl to prevent;
10)Receipts are adopted:Harvested in detoxic seedling physiological maturity using artificial, overground part cauline leafs, and luck are removed in 6-8 days before harvest Go out planting shed, pay attention to reducing epidermis injury during harvest.
2. a kind of high efficiency, low cost potato virus-free plantlet according to claim 1 and seed production technology, it is characterised in that: In step 2, the soil raw material of mixing includes by weight:Soil 160-200 parts, coarse sand 100-120 parts, farm manure 40-60 parts, Stalk plant ash 40-60 parts, 12-15 points of phosphate fertilizer, nitrogenous fertilizer 12-15 parts.
3. a kind of high efficiency, low cost potato virus-free plantlet according to claim 1 and seed production technology, it is characterised in that: In step 6, the weight ratio of cleaning up excrement water and chemical nitrogen fertilizer is 30-40:1-4.
4. a kind of high efficiency, low cost potato virus-free plantlet according to claim 1 and seed production technology, it is characterised in that: In step 5, when being transplanted to detoxic seedling, the horizontal spacing of adjacent two plants of detoxic seedlings is 6-8 centimetres, 8-10 lis of longitudinal pitch Rice.
5. a kind of high efficiency, low cost potato virus-free plantlet according to claim 1 and seed production technology, it is characterised in that: In step 8, when carrying out later stage nursing to detoxic seedling, the temperature in planting shed is maintained at 16-24 DEG C, and humidity is maintained at 60%- 70%, and pay attention to the ventilation of planting shed.
CN201711006800.4A 2017-10-25 2017-10-25 A kind of high efficiency, low cost potato virus-free plantlet and seed production technology Pending CN107616068A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112088764A (en) * 2019-10-22 2020-12-18 定西马铃薯研究所(普通合伙) Method for breeding potato breeder seeds by using coconut coir as matrix
CN113142016A (en) * 2021-04-22 2021-07-23 定西市农业科学研究院 Method for producing potato virus-free seed potato matrix by using crop straws

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102017888A (en) * 2009-09-11 2011-04-20 昆明市农业科学研究院 Virus-free basic potato seed production technology
CN106856908A (en) * 2016-12-26 2017-06-20 新昌县钧国生物技术有限公司 High efficiency, low cost potato virus-free plantlet and original silkworm egg production technology
CN107006261A (en) * 2017-05-05 2017-08-04 云南广汇种植有限公司 A kind of efficient expanding propagation method of highlands virus-free seed potato
CN107172984A (en) * 2017-03-29 2017-09-19 天津丰华裕隆农业发展有限公司 A kind of cultural method of No. 6 detoxic seedling original seeds of capital potato

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102017888A (en) * 2009-09-11 2011-04-20 昆明市农业科学研究院 Virus-free basic potato seed production technology
CN106856908A (en) * 2016-12-26 2017-06-20 新昌县钧国生物技术有限公司 High efficiency, low cost potato virus-free plantlet and original silkworm egg production technology
CN107172984A (en) * 2017-03-29 2017-09-19 天津丰华裕隆农业发展有限公司 A kind of cultural method of No. 6 detoxic seedling original seeds of capital potato
CN107006261A (en) * 2017-05-05 2017-08-04 云南广汇种植有限公司 A kind of efficient expanding propagation method of highlands virus-free seed potato

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112088764A (en) * 2019-10-22 2020-12-18 定西马铃薯研究所(普通合伙) Method for breeding potato breeder seeds by using coconut coir as matrix
CN113142016A (en) * 2021-04-22 2021-07-23 定西市农业科学研究院 Method for producing potato virus-free seed potato matrix by using crop straws

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Application publication date: 20180123