CN102017888A - Virus-free basic potato seed production technology - Google Patents

Virus-free basic potato seed production technology Download PDF

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Publication number
CN102017888A
CN102017888A CN2009100949580A CN200910094958A CN102017888A CN 102017888 A CN102017888 A CN 102017888A CN 2009100949580 A CN2009100949580 A CN 2009100949580A CN 200910094958 A CN200910094958 A CN 200910094958A CN 102017888 A CN102017888 A CN 102017888A
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cuttage
matrix
water
seedling
gram
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CN2009100949580A
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CN102017888B (en
Inventor
魏明
刘卫民
邹万君
张丽芳
朱维贤
李华
邱永寿
蒋瑜
施林
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KUNMING AGRICULTURE TECHNOLOGY RESEARCH INSTITUTE
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KUNMING AGRICULTURE TECHNOLOGY RESEARCH INSTITUTE
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    • Y02P60/216

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Abstract

The invention provides a virus-free basic potato seed production technology comprising the following three steps of seedbed treatment, cutting and management. The technology is characterized in that washed river sand is employed as the seedbed base, the management is carried out according to a certain method, and the formula of used nutrient solution is that 0.24 grams of N, 0.2 grams of P2O5 and 0.48 grams of K2O are added in each liter of water and 0.6 grams of N, 0.6 grams of P2O5 and 0.6 grams of K2O are added in each liter of water. By adopting this technology, the yield of virus-free basic potato seeds per unit area is high, and the virus-free basic potato seeds are large in size, with 68% greater than 3 grams. As a technology for planting seeds for production, the technology of the invention is low in requirement and high in survival rate.

Description

Potato detoxicating breeder's stock production technology
Technical field
The present invention relates to production technology of crops, especially potato detoxicating breeder's stock production technology.
Background technology
It mainly is to utilize stem tuber to carry out vegetative propagation that potato produces, but since plantation algebraically more after infective virus very easily, transfer the kind of exchanging of planting and planting between the family in addition, caused the potato seed degeneration, mixed, cause low, the poor quality of potato yield produced, so just have a strong impact on, restricted that potato produces and development.Go out the potato tissue cultivating seedling by tissue culture; carry out the technology that stem apex is peeled off again; the potato virus disease of having forgone; expand tissue cultural seedlings of free numerous on a large scale then; transplant in the matrix that disinfects; and carry out insulation blocking and cultivate; the potato seed of producing like this is exactly the detoxification breeder's stock; breeder's stock plantation back is an original seed; be followed successively by one-level kind, secondary kind and three grades of kinds (produce and plant); so just guaranteed the quality of potato seed in the production process, for a solid foundation has been laid in the volume increase of potato and the assurance of quality.Production detoxification breeder's stock needs higher technology just can finish with relative installations and facilities preferably, and matrix is also various, and mountain sand, humus soil, perlite etc. are arranged, culture technique is also uneven, usually cause survival rate low, unit are potato seed quantity is not high, the problem that the potato seed quality can not guarantee fully.China Patent No. " 00112983.X ", the patent of invention of title " production method of husky numerous potato detoxicating micro potato ", the production method of the numerous potato detoxicating micro potato of a kind of sand is disclosed, steps such as sterilization, the Miao Yuan by special selection matrix, matrix cultivates, shelters from heat or light, cuttage, rich water quality management reach efficient aim of high yield, but this production method operation is more complicated, micronutrient levels is more in the nutrient solution prescription that uses, increased production cost, though unit are knot potato is many, but single potato kind stature is little, and plantation back survival rate is lower.
Summary of the invention
The objective of the invention is provides a kind of potato detoxicating breeder's stock production technology in order to solve the problem that existing detoxification breeder's stock production exists, and simple to operate, cost is lower, can the survival rate height after the plantation.
Tool of the present invention discloses a kind of potato detoxicating breeder's stock production technology, comprises seedbed processing, cuttage and management three big steps, it is characterized in that specific as follows:
Prepare in step 1, seedbed, medium of seedling bed adopts the washing river sand, in advance 2 weeks matrix was caught up with flat, irrigate matrix with formaldehyde or other disinfectant, and a stifling week of envelope canopy, open canopy after the week and ventilate, turn over a matrix airing every other day, turn over 3-4 time altogether, to be sterilized dose fully after the volatilization, once more matrix is caught up with flat standby;
Step 2, cuttage, adopt the potato detoxicating tissue cultivating seedling, behind the tissue cultural seedlings of free bottle outlet medium is cleaned, before the cuttage medium of seedling bed water is irrigated, the cuttage of punching again, insert the seedling degree of depth and be seedling long 1/3, need matrix by real after in the seedling patchhole, seeding row spacing arrives 20cm, 50-200 strain/m according to required potato seed size by 5 to 10 * 10 2Plantation; After cuttage finishes, water once permeable again;
Step 3, management:
1) booth management, booth should keep neatly at any time, totally;
2) moisture will water foot with matrix before the tissue cultural seedlings of free cuttage, will water the water of revealing the exact details after the cuttage, need water 3-4 time water every day, and plant grows that can reducing behind the new root waters time counts to every day 1-2 time, successively decreases gradually later on, will note controlling water after buddingging;
3) illumination will be sheltered from heat or light with the 60-70% shade net after the tissue cultural seedlings of free cuttage is good, can take off shade net after plant survives;
4) temperature, temperature of shed are controlled at 19-28 ℃;
5) fertilising, plant is applied fertilizer after growing new root first after the cuttage, and the nutrient solution prescription that topdresses first is: every premium on currency is with N:0.24 gram+P 2O 5: 0.2 gram+K 2O:0.48 gram is used nutrient solution later on weekly, and the prescription of nutrient solution is: every premium on currency is with N:0.6 gram+P 2O 5: 0.6 gram+K 2The O:0.6 gram applies fertilizer, and whole growing is executed fertilizer 6-7 time, washes seedling with clear water immediately after each fertilising;
6) training matrix can be trained matrix when plant strain growth goes out stolon, and available substrate culture own also availablely spreads training through the ready matrix of sterilizing in advance;
7) disease control, when primary infection focus occurring, control in time, weekly, continuous 3 times.
8) results, plant reaches 60% Huang that falls can cut stake, cuts 1 week of stake back results, carries out the classification warehouse-in after the results, puts preceding warehouse in storage and answers disinfection.
The washing river sand that adopts in step 1 is distinctive washing river, Dongchuan District Xiao Jiang basin, Kunming, Yunnan Province sand, and main component wherein is a granite.
Seedbed width 1.1-1.2m in step 1, stromal thickness 20-30cm.
In step 2, the potato detoxicating tissue cultivating seedling height 6-7cm that cuttage is used.
In the present invention, medium of seedling bed adopts washing river sand, the especially distinctive washing in Dongchuan District Xiao Jiang basin, Kunming, Yunnan Province river sand, and Main Ingredients and Appearance wherein is a granite, and water-permeable and air permeable is good, and sterilization easily, thoroughly; The sand look darker, heats effective; Granite not easy-weathering attenuates, and service life is long, can use 5-6, use quartz sand as the mountain sand of main component then 1-2 will change once, thereby the matrix of using among the present invention can reduce cost; The nutrient solution prescription that fertilising is in the present invention used all is less expensive composition, based on nitrogen, phosphorus, these three kinds of macroelement of potassium, cost is lower, by method of the present invention, the output height of unit are potato detoxicating breeder's stock, stature is big, and what 3g was above accounts for 68%, as producing with kind of the less demanding and survival rate height of planting technology.
Embodiment
Embodiment 1,
Adopt distinctive washing river, Dongchuan District Xiao Jiang basin, Kunming, Yunnan Province sand, main component wherein is a granite, 2 weeks matrix was caught up with in advance flat, seedbed width 1.2m, stromal thickness 25cm, irrigate matrix with formaldehyde or other disinfectant, and a stifling week of envelope canopy, open canopy after the week and ventilate, turn over a matrix airing every other day, turn over 3-4 time altogether, to be sterilized dose fully after the volatilization, once more matrix is caught up with flat standby; Booth is selected the high-quality fly net in 0.4mm aperture for use, and seam fastens by, no breach, broken hole, import and export establish excessively between double-deck protection, the while is established sterilization pool at passage room.
Select for use height 6-7cm the potato detoxicating tissue cultivating seedling, medium should be cleaned behind the tissue cultural seedlings of free bottle outlet, the medium of seedling bed water is irrigated before the cuttage, the cuttage of punching again, insert the seedling degree of depth and be seedling long 1/3, need matrix by real after in the seedling patchhole, seeding row spacing 6 * 15cm, 112 strains/m 2Plantation.
In management, note
1) keeps the clean and tidy clean of booth
2) moisture will water foot with matrix before the tissue cultural seedlings of free cuttage, will water the water of revealing the exact details after the cuttage, and need water water every day 3-4 time, and the water yield depends on the circumstances, and plant grows, and can to reduce the number of times that waters behind the new root be every day 1-2 time, successively decreases gradually later on, will note controlling water after buddingging;
3) illumination will be sheltered from heat or light with the 60-70% shade net after the tissue cultural seedlings of free cuttage is good, can take off shade net after plant survives;
4) temperature keeps aeration-cooling in the canopy summer, and note warming winter, when temperature is low, and available little shed or manually heat, temperature remains on 19-28 ℃;
5) fertilising, plant is applied fertilizer after growing new root first after the cuttage, and the nutrient solution prescription that topdresses first is: every premium on currency is with N:0.24 gram+P 2O 5: 0.2 gram+K 2O:0.48 gram is used nutrient solution later on weekly, and nutrient solution prescription is: every premium on currency is with N:0.6 gram+P 2O 5: 0.6 gram+K 2The O:0.6 gram applies fertilizer, and whole growing generally need be executed fertile getting final product 6-7 time, washes seedling with clear water immediately after each fertilising;
6) training matrix can be trained matrix when plant strain growth goes out stolon, and available substrate culture own also availablely spreads training through the ready matrix of sterilizing in advance;
7) disease control, when primary infection focus occurring, control in time, weekly, continuous 3 times;
8) results, plant reaches 60% Huang that falls can cut stake, cuts 1 week of stake back results, carries out the classification warehouse-in after the results, puts preceding warehouse in storage and answers disinfection.
Produce 232 of 1 square metre of knot of potato detoxicating breeder's stock potatos by said method, the high-quality breeder's stock of single weight more than 3g accounts for 68%, simple to operation in this way, cost is low, the breeder's stock potato piece epidermis suberification degree height of producing, be difficult for because of epidermis damaged susceptible, individual plant knot potato number is many, and is individual big, easily used by the common producer, breeder's stock plantation back survival rate height is is easily managed and protected.

Claims (4)

1. a potato detoxicating breeder's stock production technology comprises seedbed processing, cuttage and management three big steps, it is characterized in that specific as follows:
Prepare in step 1, seedbed, medium of seedling bed adopts the washing river sand, in advance 2 weeks matrix was caught up with flat, irrigate matrix with formaldehyde or other disinfectant, and a stifling week of envelope canopy, open canopy after the week and ventilate, turn over a matrix airing every other day, turn over 3-4 time altogether, to be sterilized dose fully after the volatilization, once more matrix is caught up with flat standby;
Step 2, cuttage, adopt the potato detoxicating tissue cultivating seedling, behind the tissue cultural seedlings of free bottle outlet medium is cleaned, before the cuttage medium of seedling bed water is irrigated, the cuttage of punching again, insert the seedling degree of depth and be seedling long 1/3, need matrix by real after in the seedling patchhole, seeding row spacing arrives 20cm, 50-200 strain/m according to required potato seed size by 5 to 10 * 10 2Plantation; After cuttage finishes, water once permeable again;
Step 3, management:
1) booth management, booth should keep neatly at any time, totally;
2) moisture will water foot with matrix before the tissue cultural seedlings of free cuttage, will water the water of revealing the exact details after the cuttage, need water 3-4 time water every day, and plant grows that can reducing behind the new root waters time counts to every day 1-2 time, successively decreases gradually later on, will note controlling water after buddingging;
3) illumination will be sheltered from heat or light with the 60-70% shade net after the tissue cultural seedlings of free cuttage is good, can take off shade net after plant survives;
4) temperature, temperature of shed are controlled at 19-28 ℃;
5) fertilising, plant is applied fertilizer after growing new root first after the cuttage, and the nutrient solution prescription that topdresses first is: every premium on currency is with N:0.24 gram+P 2O 5: 0.2 gram+K 2O:0.48 gram is used nutrient solution later on weekly, and the prescription of nutrient solution is: every premium on currency is with N:0.6 gram+P 2O 5: 0.6 gram+K 2The O:0.6 gram applies fertilizer, and whole growing is executed fertilizer 6-7 time, washes seedling with clear water immediately after each fertilising;
6) training matrix can be trained matrix when plant strain growth goes out stolon, and available substrate culture own also availablely spreads training through the ready matrix of sterilizing in advance;
7) disease control, when primary infection focus occurring, control in time, weekly, continuous 3 times.
8) results, plant reaches 60% Huang that falls can cut stake, cuts 1 week of stake back results, carries out the classification warehouse-in after the results, puts preceding warehouse in storage and answers disinfection.
2. potato detoxicating breeder's stock production technology as claimed in claim 1 is characterized in that the washing river sand that adopts is distinctive washing river, Dongchuan District Xiao Jiang basin, Kunming, Yunnan Province sand in said step 1, main component wherein is a granite.
3. the production method of potato detoxicating breeder's stock as claimed in claim 1 is characterized in that seedbed width 1.1-1.2m in said step 1, stromal thickness 20-30cm.
4. potato detoxicating breeder's stock production technology as claimed in claim 1 is characterized in that in said step 2, the potato detoxicating tissue cultivating seedling height 6-7cm that cuttage is used.
CN2009100949580A 2009-09-11 2009-09-11 Virus-free potato minituber production technology Expired - Fee Related CN102017888B (en)

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CN102017888B CN102017888B (en) 2013-05-15

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102349533A (en) * 2011-08-05 2012-02-15 宁夏农林科学院 Seedbed disinfectant for controlling soil-borne disease of seed potato, preparation method and usage method thereof
CN102487684A (en) * 2011-11-29 2012-06-13 中国科学院亚热带农业生态研究所 Method for culturing potatoes in mountainous district
CN102630447A (en) * 2012-03-28 2012-08-15 常熟市滨江农业科技有限公司 Rapid propagation method of Taiwan mustard green
CN105210600A (en) * 2015-07-08 2016-01-06 甘肃凯凯农业科技发展股份有限公司 Virus-free basic potato seed hot-house culture method
CN106212225A (en) * 2016-07-15 2016-12-14 内蒙古嘉恒农业科技有限责任公司 A kind of potato seed soilless culture method
CN107616068A (en) * 2017-10-25 2018-01-23 贵州农无忧农业科技有限公司 A kind of high efficiency, low cost potato virus-free plantlet and seed production technology
CN109906900A (en) * 2019-04-01 2019-06-21 云南省农业科学院经济作物研究所 A kind of potato primary stock mating system

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102349533A (en) * 2011-08-05 2012-02-15 宁夏农林科学院 Seedbed disinfectant for controlling soil-borne disease of seed potato, preparation method and usage method thereof
CN102487684A (en) * 2011-11-29 2012-06-13 中国科学院亚热带农业生态研究所 Method for culturing potatoes in mountainous district
CN102630447A (en) * 2012-03-28 2012-08-15 常熟市滨江农业科技有限公司 Rapid propagation method of Taiwan mustard green
CN105210600A (en) * 2015-07-08 2016-01-06 甘肃凯凯农业科技发展股份有限公司 Virus-free basic potato seed hot-house culture method
CN106212225A (en) * 2016-07-15 2016-12-14 内蒙古嘉恒农业科技有限责任公司 A kind of potato seed soilless culture method
CN107616068A (en) * 2017-10-25 2018-01-23 贵州农无忧农业科技有限公司 A kind of high efficiency, low cost potato virus-free plantlet and seed production technology
CN109906900A (en) * 2019-04-01 2019-06-21 云南省农业科学院经济作物研究所 A kind of potato primary stock mating system
CN109906900B (en) * 2019-04-01 2021-10-19 云南省农业科学院经济作物研究所 Potato protospecies breeding method

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