CN107980729A - A kind of interior breeding mealybug and the method for Ban Shi encyrtids - Google Patents
A kind of interior breeding mealybug and the method for Ban Shi encyrtids Download PDFInfo
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- CN107980729A CN107980729A CN201711286885.6A CN201711286885A CN107980729A CN 107980729 A CN107980729 A CN 107980729A CN 201711286885 A CN201711286885 A CN 201711286885A CN 107980729 A CN107980729 A CN 107980729A
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- bidentate achyranthes
- mealybug
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- silk floss
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
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- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
The present invention relates to raising technology field in insectarium, and in particular to a kind of method of interior breeding mealybug and Ban Shi encyrtids.The present invention breeds Japan silk floss mealybug and Ban Shi encyrtids using the water planting root of bidentate achyranthes, the development duration of Japan silk floss mealybug and Ban Shi encyrtids can effectively be shortened, improve the survival rate of Japan silk floss mealybug and single female egg laying amount, Ban Shi encyrtids are improved to the parasitic rate and eclosion rate of Japan silk floss mealybug, indoor feeding and research especially suitable for mealybug class and its parasite.
Description
Technical field
The present invention relates to raising technology field in insectarium, and in particular to a kind of indoor breeding of mealybug and Ban Shi encyrtid
Method.
Background technology
Japan silk floss mealybug (Phenacoccus solenopsis Tinsley) is under the jurisdiction of Semiptera (Hemiptera) mealybug
Section (Pseudococcidae), be China occur in recent years a kind of serious threat field crop, ornamental plants in garden, fruit tree and
The invasive pest of the industrial crops such as vegetables safety in production, 24, the whole world, 55 sections of Zhong You of country, 200 various plants are subject to this
The feeding harm of worm.At present, which has occurring and damage in 13, China province (municipality directly under the Central Government).Ban Shi encyrtids
(Aenasius bambawalei Hayat), is under the jurisdiction of Hymenoptera (Hymenoptera) Encytidae (Eucyrtidae), is
The sociales obligatory parasitism bee of Japan silk floss mealybug, field parasitic rate are up to 50%~62%, have good biological control application
Prospect.Under laboratory condition, the expansion of Ban Shi encyrtids is numerous to rely primarily on the Japan silk floss mealybug raised on all kinds of host plants.Selection
Suitable host types and mode is reasonably cultivated, be to ensure Japan silk floss mealybug and Ban Shi encyrtids population foundation and development
Precondition, and the important foundation for making full use of Ban Shi encyrtids to carry out prevention and control to Japan silk floss mealybug.
The host range of Japan silk floss mealybug is extensive, but its suitable natural disposition and occurrence injury feelings on every kind of host plant
Condition is not quite similar.Research shows that Japan silk floss mealybug is to grass family, Malvaceae, Solanaceae, Amaranthaceae, Curcurbitaceae, great Qian sections, Pedaliaceae
It is heavier with causing harm for garden section etc..Fang Tiansong etc. (expands the research of numerous Japan silk floss mealybug and Ban Shi encyrtid host materials, Chinese forest
Disease pest, 2017) comprehensive analysis Japan silk floss mealybug coccid survival rate, the difficulty or ease for being inoculated with coccid, coccid on different host plants
Situations such as developmental state, the resistance to sustainability of host material and management, parasitic wasp harvesting difficulty or ease, material cost, development duration.As a result table
The drawbacks such as bright above host plant generally existing coccid survival rate is low, plant volume is big, production cost is high.
The content of the invention
In order to solve the above technical problem, the present invention provides a kind of indoor breeding mealybug and the method for Ban Shi encyrtids,
Substantially increase it and breed efficiency and survival rate, available for large-scale production.
Technical scheme is as follows：
A kind of method of interior breeding mealybug, comprises the following steps：The root of bidentate achyranthes is subjected to water planting, on the root of bidentate achyranthes of the water planting
Inoculation Japan silk floss mealybug is bred, and the Japan silk floss mealybug is forwarded on the fresh water planting root of bidentate achyranthes by the root of bidentate achyranthes before wilting.
Preferably, the root of bidentate achyranthes is the root of bidentate achyranthes branch of 10~20cm of length, contains 4~6 blades on the root of bidentate achyranthes branch.
It is furthermore preferred that the root of bidentate achyranthes branch is the morning 7:00~9:The fresh braches of 00 picking.
Preferably, the water planting is distilled water or the indolebutyric acid solution of 0.2mg/L with water planting liquid.
It is furthermore preferred that the root of bidentate achyranthes bough water planting of the inoculation Japan silk floss mealybug replaces fresh water planting root of bidentate achyranthes branch after 16~32 days
Bar.
Preferably, the age of the inoculation Japan silk floss mealybug is an age, and inoculum concentration is every plant of root of bidentate achyranthes 4~20.
Present invention also offers a kind of method of indoor breeding Ban Shi encyrtids, comprise the following steps：The root of bidentate achyranthes is subjected to water
Training, Japan silk floss mealybug is inoculated with the root of bidentate achyranthes of the water planting, will be inoculated with the root of bidentate achyranthes plant gauze of Japan silk floss mealybug with it is extraneous every
From the Ban Shi encyrtids that first emergence is blown into the gauze and has been mated are bred.
Preferably, the Japan silk floss mealybug tritonymph or female adult pest of the inoculation, inoculum concentration are every plant of root of bidentate achyranthes 10~20.
Preferably, the root of bidentate achyranthes is the root of bidentate achyranthes branch of 10~20cm of length, contains 4~6 blades on the root of bidentate achyranthes branch.
Preferably, the water planting liquid of the root of bidentate achyranthes is distilled water or the indolebutyric acid solution of 0.2mg/L.
Compared with prior art, the present invention has the following advantages：
The present invention breeds Japan silk floss mealybug using the water planting root of bidentate achyranthes.The root of bidentate achyranthes (Achyranthes bidentata
Blume be) Amaranthaceae, Achyranthes herbaceos perennial, and with aboundresources, after growth is rapid, stalk is in vitro easily survival and
The advantages that being easy to take root.Present invention discover that the root of bidentate achyranthes is the Suitable Host of Japan silk floss mealybug, the root of bidentate achyranthes is carried out water planting can be as helping
The good host plant of mulberry silk floss mealybug breeding.The water planting root of bidentate achyranthes can overcome that basin soil cultivation is of high cost, it is big to take up space, to environment at the same time
It is required that harsh, easy infection disease and other species pests, the mealybug easily drawback such as escape.
The method of breeding mealybug in interior of the invention, can effectively reduce mealybug escape, while has and easily connect worm, mealybug development
Situation is good, resistance to deposits the advantages that time is long, store keeping is convenient, natural enemy is easy to harvesting and the cost of material is low.The present invention uses water planting
Method, which expands numerous root of bidentate achyranthes plant, can effectively shorten the development duration of Japan silk floss mealybug, Japan silk floss mealybug always going through on root of bidentate achyranthes plant
Phase is 46.40 days, wherein 6.20 days 1 ages, 2 6.00 days ages, 3 5.80 days ages, female adult pest 28.40 days.Wherein 3 age nymphs and it is female into
Worm development duration is the most suitable Host Instar of Ban Shi encyrtids, is suitable for the breeding of Ban Shi encyrtids.The method breeding of the present invention
The survival rate of Japan silk floss mealybug is higher than 55.00%, and the female egg laying amount of list on root of bidentate achyranthes plant is 255.33~345.00, breeding
Effect is preferable.
The present invention breeds Japan silk floss mealybug on water planting root of bidentate achyranthes plant, the parasitism for Ban Shi encyrtids.Shorten Ban Shi
The development duration of encyrtid.From laying eggs to sprouting wings, the undergone time is only 12.40d to Ban Shi encyrtids.The method of the present invention is bred
Ban Shi encyrtid female adults longest-lived reach 15.20d, reach 100% to the parasitic rate of mealybug, eclosion rate for 91.99~
98.66%, filial generation ratio is (female：It is male) ratio is 1.98~3.36, it is extremely suitable for the breeding of Ban Shi encyrtids.
Indoor population expansion of the root of bidentate achyranthes ciltivating process of the present invention for Japan silk floss mealybug and Ban Shi encyrtids is numerous, has and relatively attaches most importance to
The meaning wanted, indoor feeding and research especially suitable for mealybug class and its parasite.
Embodiment
The present invention provides a kind of method of indoor breeding mealybug, comprise the following steps：The root of bidentate achyranthes is subjected to water planting, described
Japan silk floss mealybug is inoculated with the root of bidentate achyranthes of water planting to be bred, and the Japan silk floss mealybug is forwarded to fresh water before the root of bidentate achyranthes wilting
Train on the root of bidentate achyranthes.
In the present invention, the root of bidentate achyranthes be Japan silk floss mealybug Suitable Host plant, to the root of bidentate achyranthes carry out water planting to Japan silk floss mealybug into
Row breeding, can shorten the development duration of Japan silk floss mealybug, improve the survival rate of Japan silk floss mealybug and single female egg laying amount, improve Japan
The breeding efficiency of continuous mealybug.
In the present invention, the water planting root of bidentate achyranthes can be the whole strain root of bidentate achyranthes, or the fresh root of bidentate achyranthes branch of picking, by whole strain
The root of the root of bidentate achyranthes or the cut end of root of bidentate achyranthes branch, which are immersed in water planting liquid, carries out water planting.In the specific embodiment of the invention, using ox
Knee branch carries out water planting.Root of bidentate achyranthes branch derives from a wealth of sources, and easily survives and takes root after stalk is in vitro, is more suitable for water planting breeding powder
A red-spotted lizard.
In the present invention, the fresh root of bidentate achyranthes branch of preferred acquisition carries out water planting.The preferably morning 7:00~9:00 picking it is new
Fresh branch, for the root of bidentate achyranthes branch of other times collection, the root of bidentate achyranthes branch freshness of above-mentioned period collection is more preferable, leaf
The time that piece holding is unfolded is longer.
In the present invention, the length of the root of bidentate achyranthes branch is preferably 10~20cm, more preferably 14~18cm.It is preferred that root of bidentate achyranthes branch
The number of blade contained on bar is 4~6.Suitable branch length and the suitable number of blade can keep root of bidentate achyranthes branch in water planting mistake
Water absorption and transpiration rate in journey maintain equilibrium state, effectively extend the water planting time of root of bidentate achyranthes branch.
In the present invention, the sterilized rear progress water planting of root of bidentate achyranthes branch of preferred acquisition.Sterilization method can use this area
Routine disinfection mode carries out disinfection root of bidentate achyranthes branch.In the present invention, it is preferred to carried out disinfection with carbendazim.Specifically sterilization method is
The root of bidentate achyranthes branch notch of collection is immersed 10~20s is sterilized in 50% carbendazol wettable powder, 500~1000 times of dilutions, more
It is preferred that the extension rate of 50% carbendazol wettable powder is 800 times of liquid, disinfecting time is more preferably 15s.Under this condition can
It is enough effectively to carry out disinfection to root of bidentate achyranthes branch, avoid the pollution or rotten of branch during water planting.
In the present invention, it is preferably distilled water or the indoles fourth of 0.2mg/L that used water planting liquid during water planting is carried out to the root of bidentate achyranthes
Acid solution.The present invention screens a variety of water planting liquid, obtains with the indolebutyric acid solution of distilled water or 0.2mg/L to root of bidentate achyranthes branch
Bar carries out water planting, and the rooting rate and radical of root of bidentate achyranthes branch, root long are optimal, and the water planting time is longer, and water planting twotooth achyranthes leaf is in laboratory
Under the conditions of can survive 32 days.
In the present invention, Japan silk floss mealybug is connect in time on the water planting root of bidentate achyranthes.Usually root of bidentate achyranthes plant is inserted in water planting liquid
Connect Japan silk floss mealybug.It is preferred that the age for connecing Japan silk floss mealybug is an age.Select age Japan's silk floss that physiological status of the same size is good
Mealybug is connected on the water planting root of bidentate achyranthes.In the present invention, 4~20 Japan silk floss mealybugs are preferably connect on every plant of water planting root of bidentate achyranthes, more preferably 6~
10.It will be bred under the water planting root of bidentate achyranthes proper environment disposed within for being connected to Japan silk floss mealybug.It is preferred that 23~28 DEG C of room temperature,
Relative humidity 70~80%, photoperiod L16:Raising in D8 air-conditioned rooms.
The water planting root of bidentate achyranthes wilting before, the Japan silk floss mealybug colonized on root of bidentate achyranthes branch is gone on the fresh water planting root of bidentate achyranthes after
Continuous breeding.It is preferred that the time transferred to be inoculated with the root of bidentate achyranthes bough water planting of Japan silk floss mealybug after 16~32 days, is more preferably 20~25
My god.
The present invention can also use gauze by parasitic Japan silk floss mealybug when breeding Japan silk floss mealybug with the water planting root of bidentate achyranthes
The water planting root of bidentate achyranthes is isolated with the external world, prevents the escape of Japan silk floss mealybug and the intrusion of insect parasite.It is preferred that the density of gauze is not
Less than 120 mesh.
The method of the above-mentioned indoor breeding mealybug of the present invention is equally applicable to breeding Ban Shi encyrtids.Interior breeding class of the invention
The method of family name encyrtid includes, and the root of bidentate achyranthes is carried out water planting, Japan silk floss mealybug is inoculated with the root of bidentate achyranthes of the water planting, will be inoculated with Japan
The root of bidentate achyranthes plant of continuous mealybug is isolated from the outside with gauze, be blown into the gauze just sprout wings and the Ban Shi encyrtids that have mated into
Row breeding.Ban Shi encyrtids can lay eggs by host of the Japan silk floss mealybug on the root of bidentate achyranthes, breeding.
In the present invention, the Japan silk floss mealybug being preferably inoculated with is tritonymph or female adult pest, and the Japan silk floss mealybug of the age connects
Kind is in the parasitism for being easy to Ban Shi encyrtids on the root of bidentate achyranthes.Or the Japan silk floss mealybug of other ages is inoculated with, treat Japan's silk floss powder on the root of bidentate achyranthes
A red-spotted lizard is developed to tritonymph or female adult pest and accesses Ban Shi encyrtids again.In the present invention, the inoculum concentration of preferably Japan silk floss mealybug is every
The strain root of bidentate achyranthes 10~20, more preferably 13~17, it is parasitic for Ban Shi encyrtids adult.
The gauze used in the isolation root of bidentate achyranthes is not particularly limited in the present invention, preferably the density of gauze be not less than 120 mesh,
So that Ban Shi encyrtids are movable near the water planting root of bidentate achyranthes, host can be accurately found easy to it.Meanwhile the setting of gauze can also be prevented
The only escape of Japan silk floss mealybug and the intrusion of other natural enemies.
The present invention can immediately access Ban Shi encyrtids after Japan silk floss mealybug is accessed, or wait Japan silk floss mealybug to breed to one
Ban Shi encyrtids are accessed again after determining degree.It is preferred that jumped in the tritonymph phase of Japan silk floss mealybug or female adult pest phase access Ban Shi small
Bee, to improve parasitic rate.The present invention is not particularly limited the quantity for being blown into Ban Shi encyrtids, can be according to the quantity of the water planting root of bidentate achyranthes
And the quantity of Japan silk floss mealybug sets suitable Ban Shi encyrtids access amount.In the present invention, preferably every plant of water planting root of bidentate achyranthes accesses 1 pair
The Ban Shi encyrtids for just sprouting wings and having mated, parasitic Japan silk floss mealybug tritonymph or female adult pest be not low preferably on the water planting root of bidentate achyranthes
In 10.
In the method for breeding Ban Shi encyrtids in interior of the invention, the water planting mode of the root of bidentate achyranthes and the indoor breeding powder of foregoing description
The mode of agent is identical, refers to foregoing description and carries out the water planting root of bidentate achyranthes in Ban Shi encyrtid breedings.
The present invention is not particularly limited the device of indoor breeding mealybug and Ban Shi encyrtids, numerous using the routine of this area
Educate device.Water planting is carried out to the root of bidentate achyranthes such as in hydroponic device, contains component, the splendid attire for fixing the root of bidentate achyranthes in the hydroponic device
The component of water planting liquid and gauze component of covering water planting root of bidentate achyranthes plant etc..
For the object, technical solutions and advantages of the present invention are more clearly understood, with reference to embodiment to the present invention into
Row detailed description, but they cannot be interpreted as limiting the scope of the present invention.
Embodiment 1
The influence of different time sections and different length root of bidentate achyranthes branch to water planting
Vigorous period (5~September) selection 7 is grown in the root of bidentate achyranthes:00~9:00 (20~25 DEG C), 11:00~13:00 (35~
40 DEG C) and 17:00~19:00 (25~30 DEG C) period picked+2 leaves of 5cm stalks ,+4 leaves of 10cm stalks, 15cm stems respectively
Each 10 sections of the root of bidentate achyranthes plant branches and leaves of+8 leaves of+6 leaves of stalk and 20cm stalks, after clear water moisturizing 24h, according to its stalk and blade
Fresh-keeping situation carry out grading evaluation.Wherein, the grade scale of fresh-keeping situation is as follows：Blade and the normal fresh-keeping degree of stalk
Highest, the fresh-keeping degree that less than half blade is wilted are taken second place, and the fresh-keeping degree that blade and stalk are wilted is minimum.
The different plucking time section root of bidentate achyranthes freshness of table 1 compare
Note：I, blade and cane are normal；
II, the blade of fewer than half quantity are wilted, and cane is normal；
III, the blade of more than half are wilted, and cane is slightly wilted；
IV, blade and cane are wilted.
From table 1 it follows that the different picking periods have considerable influence for the freshness of root of bidentate achyranthes plant.Wherein,
7:00~9:The plant freshness highest of 00 picking, 11:00~13:The plant freshness of 00 picking is minimum.It is in addition, different
Culm length and plant leaf quantity can also influence the freshness of root of bidentate achyranthes plant, 10~20cm long stalks and reservation 4~6
The root of bidentate achyranthes branch of blade is more fresh.Therefore, following embodiments are by 7:00~9:10~20cm long the stalks of 00 period picking
And retain material of the root of bidentate achyranthes branch of 4~6 blades as water planting.
Embodiment 2
Root of bidentate achyranthes branch disinfects effect
50% carbendazim, 100 times, 500 times, 1000 times and 2000 times liquid are immersed into root of bidentate achyranthes stalk end after trimming respectively
Middle 5s, 10s, 20s and 40s carry out wound disinfection, then immerse branch end 5cm sterilized or so in ordinary tap water,
Check floccule whether occur around wound face after 24h, whether water body is muddy.
Root of bidentate achyranthes infection of incisional wound situation (%) after the processing of 2 various concentrations of table, 50% carbendazol
Note：Different capitalizations, represent under same chemicals treatment, and different disposal time difference is notable；Different lowercases,
Represent under same processing time significant difference between different agents.
From Table 2, it can be seen that different carbendazim concentrations and processing time, have for the infection state of root of bidentate achyranthes notch
There is considerable influence, excessive concentration or too low, processing time is long or too short, is unfavorable for notch disinfection.Wherein, notch immerses
The Disinfection Effect of 10~20s is optimal in 50% 500~1000 times of carbendazol wettable powder dilution.
Embodiment 3
Select different water planting liquid to carry out preliminary experiment, verify its influence to root of bidentate achyranthes water planting.
The water planting liquid of selection is respectively：Distilled water, IBA (0.2mg/L), NAA (0.05mg/L), compound (NAA (0.05mg/
L) with root-inducing powder (1.0mg/L) with 1:1 volume is mixed), tap water (《Standards for drinking water quality》：GB5749-
And root-inducing powder (1.0mg/L) 2006).
Select above-mentioned water planting liquid to carry out water planting to the in vitro stalk of the root of bidentate achyranthes respectively, record its situation of taking root day by day, and count raw
Root rate, radical and total root long.
The influence that the different water planting liquid of table 3 and processing time take root the root of bidentate achyranthes
Note：Different capitalizations, represented under same processing time, significant difference between different disposal medicament；Different small letters
Letter, represents under same treatment agent significant difference between the different disposal time.
From table 3 it can be seen that different taking root liquids and processing time take root the root of bidentate achyranthes, there are considerable influence.It is raw in the case of 6d
Root rate and radical are with optimal under the conditions of distilled water and tap water, respectively up to 75.00%~87.50% and 12.50~13.25
Bar, but average root long indifference between different rooting treatments；In the case of 10d, rooting rate and radical indifference between different rooting treatments,
But average root long is compared with other processing length under distilled water, IBA and water treatment；In the case of 16d, the processing knot of distilled water and IBA
Fruit and handling result difference during 6d and 10d is not notable, and the value of other taking root liquids is zero.
Embodiment 4
Vigorous period selection 7 is grown in the root of bidentate achyranthes:00~9:00 (20~25 DEG C) period picked root of bidentate achyranthes branch, root of bidentate achyranthes branch
Length is 15cm, with 6 leaves.Root of bidentate achyranthes branch lower end under picking need to be immersed in the water immediately；Under water to root of bidentate achyranthes branch
End is tiltedly cut in 45° angle degree, tiltedly cuts position under tubercle at 2.0~3.0cm, retains root of bidentate achyranthes branch 12cm；By the branch after trimming
Bar lower end 5.0cm immerses 10s in 50% carbendazol wettable powder, 1000 times of dilutions, and root of bidentate achyranthes branch is fixed on and is loaded with steaming
In the hydroponic device of distilled water.20 age Japan silk floss mealybugs of the same size are connect on the water planting root of bidentate achyranthes, are breathed freely gauze with 120 purposes
The water planting root of bidentate achyranthes after worm will be connect to cover, be isolated from the outside.It is placed on 23~28 DEG C of room temperature, relative humidity 70~80%, photoperiod
For L16:Raising in D8 air-conditioned rooms.Before the wilting of the water planting root of bidentate achyranthes, an age Japan silk floss mealybug is connected to according to the method described above fresh
Cycle cultivation is carried out on the water planting root of bidentate achyranthes.
Embodiment 5
Vigorous period selection 7 is grown in the root of bidentate achyranthes:00~9:00 (20~25 DEG C) period picked root of bidentate achyranthes branch, root of bidentate achyranthes branch
Length is 20cm, with 8 leaves.Root of bidentate achyranthes branch lower end under picking need to be immersed in the water immediately；Under water to root of bidentate achyranthes branch
End is tiltedly cut in 45° angle degree, tiltedly cuts position under tubercle at 2.0~3.0cm, retains root of bidentate achyranthes branch 15cm；By the branch after trimming
Bar lower end 5.0cm immerses 20s in 50% carbendazol wettable powder, 500 times of dilutions, and root of bidentate achyranthes branch is fixed on and is loaded with
In the hydroponic device of 0.2mg/L indolebutyric acid solution.4 age Japan silk floss mealybugs of the same size are connect on the water planting root of bidentate achyranthes, are used
120 purposes breathe freely gauze will connect worm after the water planting root of bidentate achyranthes cover, be isolated from the outside.It is placed on 23~28 DEG C of room temperature, relative humidity
70~80%, photoperiod L16:Raising in D8 air-conditioned rooms.Before the wilting of the water planting root of bidentate achyranthes, by an age Japan silk floss mealybug according to above-mentioned
Method is connected to progress cycle cultivation on the fresh water planting root of bidentate achyranthes.
Embodiment 6~9
6,8,10 and 12 age Japan silk floss mealybugs of the same size are connect respectively on the water planting root of bidentate achyranthes, respectively as embodiment
6~9.Remaining method is same as Example 5.
Comparative example 1
10s in 50% carbendazol wettable powder, 1000 times of dilutions is immersed into the root of root of bidentate achyranthes seedling, by root of bidentate achyranthes seedling
Transplant in the flowerpot equipped with garden mould, contain one plant of root of bidentate achyranthes plant in every basin.24 it is small when after, on the root of bidentate achyranthes plant of transplant survival
Connect 20 age Japan silk floss mealybugs of the same size, with 120 purposes breathe freely gauze will connect worm after the potted plant root of bidentate achyranthes cover, with the external world
Isolation.It is placed on 23~28 DEG C of room temperature, relative humidity 70~80%, photoperiod L16:Raising in D8 air-conditioned rooms.In potted plant ox
Before knee is wilted, an age Japan silk floss mealybug is connected to progress cycle cultivation on the fresh potted plant root of bidentate achyranthes according to the method described above.
Comparative example 2
In the vigorous period selection 7 of cotton growth:00~9:00 (20~25 DEG C) period picked the cotton leaf with handle.By cotton
The lower end of floral leaf handle need to be immersed in the water immediately；The end of cotton petiole is tiltedly cut in 45° angle degree under water；By the cotton after trimming
10s in 50% carbendazol wettable powder, 1000 times of dilutions is immersed in petiole lower end, and cotton petiole is fixed on and is loaded with distillation
In the hydroponic device of water.20 age Japan silk floss mealybugs of the same size are connect on water planting cotton, breathing freely gauze with 120 purposes will
Connect the water planting cotton after worm to cover, be isolated from the outside.It is placed on 23~28 DEG C of room temperature, relative humidity 70~80%, photoperiod are
L16:Raising in D8 air-conditioned rooms.Before water planting cotton is wilted, an age Japan silk floss mealybug is connected to fresh water according to the method described above
Train and cycle cultivation is carried out on cotton.
Comparative example 3
Vigorous period selection 7 is grown in potato:00~9:00 (20~25 DEG C) period picked potato branch, potato branch
Length is 15cm, with 6 leaves.Potato branch lower end under picking need to be immersed in the water immediately；Under water to potato branch
End is tiltedly cut in 45° angle degree, tiltedly cuts position under tubercle at 2.0~3.0cm, retains potato branch 12cm；By the branch after trimming
10s in 50% carbendazol wettable powder, 1000 times of dilutions is immersed in bar lower end, and potato branch is fixed on and is loaded with distilled water
Hydroponic device in.20 age Japan silk floss mealybugs of the same size are connect on water planting potato, will be connect with 120 purposes gauze of breathing freely
Water planting potato after worm covers, and is isolated from the outside.It is placed on 23~28 DEG C of room temperature, relative humidity 70~80%, photoperiod are
L16:Raising in D8 air-conditioned rooms.Before the wilting of water planting potato, an age Japan silk floss mealybug is connected to fresh water according to the method described above
Cycle cultivation is carried out on ridging beans.
Comparative example 4
In the vigorous period selection 7 of tomato growth:00~9:00 (20~25 DEG C) period picked tomato branch, tomato branch
Length is 15cm, with 6 leaves.Tomato branch lower end under picking need to be immersed in the water immediately；Under water to tomato branch
End is tiltedly cut in 45° angle degree, tiltedly cuts position under tubercle at 2.0~3.0cm, retains tomato branch 12cm；By the branch after trimming
10s in 50% carbendazol wettable powder, 1000 times of dilutions is immersed in bar lower end, and tomato branch is fixed on and is loaded with distilled water
Hydroponic device in.20 age Japan silk floss mealybugs of the same size are connect on water planting tomato, will be connect with 120 purposes gauze of breathing freely
Water planting tomato after worm covers, and is isolated from the outside.It is placed on 23~28 DEG C of room temperature, relative humidity 70~80%, photoperiod are
L16:Raising in D8 air-conditioned rooms.Before water planting tomato is wilted, an age Japan silk floss mealybug is connected to fresh water according to the method described above
Train and cycle cultivation is carried out on tomato.
Embodiment 10
Vigorous period selection 7 is grown in the root of bidentate achyranthes:00~9:00 (20~25 DEG C) period picked root of bidentate achyranthes branch, root of bidentate achyranthes branch
Length is 13cm, with 4 leaves.Root of bidentate achyranthes branch lower end under picking need to be immersed in the water immediately；Under water to root of bidentate achyranthes branch
End is tiltedly cut in 45° angle degree, tiltedly cuts position under tubercle at 2.0~3.0cm, retains root of bidentate achyranthes branch 10cm；By the branch after trimming
Bar lower end 5.0cm immerses 10s in 50% carbendazol wettable powder, 1000 times of dilutions, and root of bidentate achyranthes branch is fixed on and is loaded with steaming
In the hydroponic device of distilled water.15 three ages Japan silk floss mealybug nymphs of the same size are connect on the water planting root of bidentate achyranthes, are breathed freely with 120 purposes
The water planting root of bidentate achyranthes after gauze will connect worm covers, and is isolated from the outside.Then sprout wings (1-2d) at the beginning of 1 pair being blown into the device and handed over
The Ban Shi encyrtids matched somebody with somebody, are placed on 23~28 DEG C of room temperature, relative humidity 70~80%, photoperiod L16:Raised in D8 air-conditioned rooms
Support.
Embodiment 11
15 Japan silk floss mealybug female adult pests of the same size are connect on the water planting root of bidentate achyranthes, remaining method is the same as embodiment 10.
Comparative example 5~7
15 three ages Japan silk floss mealybug nymphs of the same size are connect on the hydroponic plant of comparative example 2~3 respectively, with 120
Purpose breathe freely gauze will connect worm after the water planting root of bidentate achyranthes cover, be isolated from the outside.Then sprout wings (1- at the beginning of 1 pair being blown into the device
2d) and the Ban Shi encyrtids that have mated, 23~28 DEG C of room temperature, relative humidity 70~80%, photoperiod L16 are placed on:D8 is empty
Transfer house interior raising.
Embodiment 12
Compare the influence of different host plant types of rearing and floristics to Japan silk floss mealybug escape amount
Embodiment 4 and documents 1~4 are reprocessed 5 times, remaining mealybug quantity is counted after counting 24h.It the results are shown in Table
4。
Mealybug escape difference in 4 different planting of table and host plant species
| Host plant | Remaining mealybug quantity (head) | |
| Embodiment 4 | The water planting root of bidentate achyranthes | 19.20±0.37a |
| Comparative example 1 | The potted plant root of bidentate achyranthes | 11.00±0.71b |
| Comparative example 2 | Water planting cotton | 12.80±0.58b |
| Comparative example 3 | Water planting potato | 8.80±0.58c |
| Comparative example 4 | Water planting tomato | 6.20±0.37d |
Note：Different lowercases, represent significant difference between different planting/host types.
As can be seen from Table 4 Japan silk floss mealybug on water planting twotooth achyranthes leaf almost without escape phenomenon, and potted plant root of bidentate achyranthes seedling,
The mealybug that water planting cotton cauline leaf, water planting potato cauline leaf have half or so is escaped, and has more than 2/3 powder on water planting tomato stem-leaf
A red-spotted lizard is escaped.
Embodiment 13
Evaluating the breeding on Japan silk floss mealybug of different host plants influences
During Japan silk floss mealybug 1 age nymphal development is to female adult pest, compare 4 kinds of host plant Rooted Cuttings artificial infections
Difficulty or ease, coccid developmental state, resistance to sustainability, store keeping, natural enemy harvesting complexity, material cost etc.；Clearly difference host
The influence that water culture seedling is survived mealybug, develops and bred.
The numerous index of expansion of the different water planting host plant raising Japan silk floss mealybugs of table 5
Note：Different lowercases, represent significant difference between different host types, similarly hereinafter.
As can be seen from Table 5, during worm is connect, root of bidentate achyranthes blade is open and flat, and quantity is more, easily connects worm；Cotton leaf is larger, connects
Worm is also relatively easy to；Potato and tomato leaf surface area are small, and inoculation nymph easily drops, and connects worm relative difficulty.Water planting twotooth achyranthes leaf
It can survive in laboratory conditions 32 days, far above other host plant species, can effectively reduce and replace host plant number, have
Beneficial to the development and breeding for saving man-hour and promotion mealybug.Difficulty is harvested from worm development degree, Room management difficulty and natural enemy is connect
For, water planting twotooth achyranthes leaf is superior to other species.In addition, the root of bidentate achyranthes connects as a kind of common wild plant, the cost of raw material
It is bordering on 0.
Mealybug survival rate, development duration and proliferation bias on the different water planting host plant of table 6
As can be seen from Table 6, there are larger difference for Japan silk floss mealybug survival, development and breeding on different water planting host plants
It is different.Wherein, still excellent with water planting twotooth achyranthes leaf, its mealybug survival rate, (Ban Shi encyrtids are most for 3 age nymphs+female adult pest development duration
Suitable Host Instar) and singly female egg laying amount is respectively 55.00%, 34.00d and 328.00.
In general, the survival rate of 1 age mealybug nymph is relatively low.Wherein, different mealybug density are to influence the weight of its survival rate
Want factor.Embodiment 5~9 is respectively connected to 4,6,8,10 and 12 Japan silk floss mealybug 1 age nymphs on the water planting root of bidentate achyranthes,
Often processing is repeated 3 times；Day by day the situation of record death and husking, counts its development duration and the development time and female adult pest in each age
Egg laying amount.
Influence of the different mealybug nymphal densities of table 7 for survival rate and egg laying amount
| Mealybug density | Survival rate (%) | Egg laying amount (grain) | |
| Embodiment 5 | 4 | 66.67±16.67a | 311.00±18.04a |
| Embodiment 6 | 6 | 94.44±5.56a | 345.00±11.93a |
| Embodiment 7 | 8 | 70.83±15.02a | 330.00±20.84a |
| Embodiment 8 | 10 | 66.67±18.56a | 342.00±15.87a |
| Embodiment 9 | 12 | 58.33±17.35a | 255.33±5.36b |
Note：Different lowercases, represent significant difference between different mealybug density.
As can be seen from Table 7, singly female egg laying amount is there are considerable influence to it for different mealybug density, wherein 1 age mealybug nymph
When density is 4~10, single female egg laying amount is 311.00~342.00, when far above mealybug nymphal density being 12.Meanwhile
During 1 age nymphal development to female adult pest of feeding root of bidentate achyranthes blade, its single female egg laying amount be higher than tomato and eggplant (Huang Fang etc.,
2011), the egg laying amount of cotton (Lu Dongdong and business's break are military, 2016) and Japan's tender leaf (king is superfine, 2016).
8 mealybug ovum of table, the development duration of nymph and female adult pest on root of bidentate achyranthes plant
Mealybug development duration shortens, and may be conducive to mealybug feeding digestion with host plant and promote ontogeny to accelerate to have
Close.And for raising Ban Shi encyrtids, suitable host plant species is selected, the development for shortening Japan silk floss mealybug as far as possible is gone through
Phase, obtains the Host Instar (3 age nymphs and female adult pest) for being adapted to its parasitic, is greatly improved reproductive efficiency.Table 8 the result shows that, 1
Development duration of~2 age Japan silk floss mealybugs on root of bidentate achyranthes plant is 12.2d, is shorter than and goes through phase (16.53d) and gumbo on pumpkin
On go through the phase (12.8d), with cotton (the most suitable host of Japan silk floss mealybug) go through phase indifference (Huang Fang etc., 2011；Deer east
East and business's break are military, and 2016).
Embodiment 14
Evaluating the breeding on Ban Shi encyrtids of different host plants influences
Ban Shi encyrtids are inoculated with according to the method for embodiment 10~11 and comparative example 5~7,72h is afterwards by Ban Shi encyrtids
Suction out, Japan silk floss mealybug deadlock a red-spotted lizard quantity, bee eclosion rate and Female offspring ratio are counted after 14d.Assess different host plant water plantings
Effect of the seedling to Ban Shi encyrtids development duration, queen bee service life and grade of fit.
The development of the difference water planting host plant Shang Banshi of table 9 encyrtids, queen bee service life and grade of fit difference
As can be seen from Table 9, different water planting host plants are equal to the development of Ban Shi encyrtids, queen bee service life and Parasitic Fitness
There are influence.It is most short using the time that mealybug on water planting twotooth achyranthes leaf is undergone by the Ban Shi encyrtids of host from oviposition to emergence
It is (be only 12.40d), female adult longest-lived (reaching 15.20d), (female to the parasitic rate (96.00%) and filial generation ratio of mealybug：Male=
2.97) equal highest.
Parasitic rate, eclosion rate and the filial generation ratio of Japan silk floss mealybug on the 10 Ban Shi encyrtid parasitism roots of bidentate achyranthes of table
| Host Instar | Parasitic rate (%) | Eclosion rate (%) | Filial generation ratio is (female：It is male) | |
| Embodiment 10 | 3 age nymphs | 95.97±1.32a | 98.67±1.33a | 3.36±0.97a |
| Embodiment 11 | Female adult pest | 96.00±1.87a | 95.89±1.03a | 2.97±0.60a |
Japan silk floss mealybug 3 age nymph and female adult pest on the Ban Shi encyrtid parasitism roots of bidentate achyranthes, its parasitic rate close to 100%,
Eclosion rate is above 90%, and filial generation ratio is 2.97~3.36, far above in potato (what Lang sweet smell etc., 2012) and cotton (Huang Jun
Deng 2012；It is beautiful etc., 2014；Zhang et al., 2016) parasitic rate, eclosion rate and filial generation ratio on.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of method of interior breeding mealybug, comprises the following steps：The root of bidentate achyranthes is subjected to water planting, is connect on the root of bidentate achyranthes of the water planting
Kind Japan silk floss mealybug is bred, and the Japan silk floss mealybug is forwarded on the fresh water planting root of bidentate achyranthes by the root of bidentate achyranthes before wilting.
2. according to the method described in claim 1, it is characterized in that, the root of bidentate achyranthes be 10~20cm of length root of bidentate achyranthes branch, institute
It is 4~6 to state the number of blade contained on root of bidentate achyranthes branch.
3. according to the method described in claim 2, it is characterized in that, the root of bidentate achyranthes branch is the morning 7:00~9:00 picking it is new
Fresh branch.
4. according to the method described in claim 1, it is characterized in that, the water planting with water planting liquid is distilled water and 0.2mg/L
Indolebutyric acid solution.
5. according to the method in claim 2 or 3, it is characterised in that the root of bidentate achyranthes bough water planting of the inoculation Japan silk floss mealybug
Fresh water planting root of bidentate achyranthes branch is replaced after 16~32 days.
6. according to the method described in claim 1, it is characterized in that, it is described inoculation Japan silk floss mealybug age be an age, be inoculated with
Measure as every plant of root of bidentate achyranthes 4~20.
7. a kind of method of interior breeding Ban Shi encyrtids, comprises the following steps：The root of bidentate achyranthes is subjected to water planting, in the ox of the water planting
Above-knee inoculation Japan silk floss mealybug；The root of bidentate achyranthes for being inoculated with Japan silk floss mealybug is isolated from the outside with gauze, is blown into the gauze just
The Ban Shi encyrtids for sprouting wings and having mated are bred.
8. the method according to the description of claim 7 is characterized in that the Japan silk floss mealybug of the inoculation for tritonymph or it is female into
Worm, inoculum concentration are every plant of root of bidentate achyranthes 10~20.
9. according to the method according to the description of claim 7 is characterized in that the root of bidentate achyranthes is the root of bidentate achyranthes branch of 10~20cm of length
Bar, contains 4~6 blades on the root of bidentate achyranthes branch.
10. the method according to the description of claim 7 is characterized in that the water planting liquid of the root of bidentate achyranthes is distilled water or 0.2mg/L
Indolebutyric acid solution.
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