CN110521674A - A kind of indoor feeding method of Ban Shi encyrtid - Google Patents
A kind of indoor feeding method of Ban Shi encyrtid Download PDFInfo
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- CN110521674A CN110521674A CN201910791379.5A CN201910791379A CN110521674A CN 110521674 A CN110521674 A CN 110521674A CN 201910791379 A CN201910791379 A CN 201910791379A CN 110521674 A CN110521674 A CN 110521674A
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- 241001147399 Encyrtidae Species 0.000 title claims abstract description 76
- 238000000034 method Methods 0.000 title claims abstract description 23
- 230000003071 parasitic effect Effects 0.000 claims abstract description 67
- 241001465977 Coccoidea Species 0.000 claims abstract description 64
- 244000061456 Solanum tuberosum Species 0.000 claims abstract description 61
- 235000002595 Solanum tuberosum Nutrition 0.000 claims abstract description 61
- 241000628997 Flos Species 0.000 claims abstract description 44
- 230000035784 germination Effects 0.000 claims abstract description 32
- 241000270322 Lepidosauria Species 0.000 claims abstract description 14
- 238000011160 research Methods 0.000 claims abstract description 7
- 238000012360 testing method Methods 0.000 claims abstract description 6
- 241000238631 Hexapoda Species 0.000 claims description 45
- 230000001419 dependent effect Effects 0.000 claims description 31
- 230000000384 rearing effect Effects 0.000 claims description 23
- 229920000742 Cotton Polymers 0.000 claims description 16
- 239000004576 sand Substances 0.000 claims description 16
- 241000607479 Yersinia pestis Species 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 238000007598 dipping method Methods 0.000 claims description 7
- 239000000843 powder Substances 0.000 claims description 5
- 235000012015 potatoes Nutrition 0.000 claims description 3
- 238000004378 air conditioning Methods 0.000 claims 1
- 235000015097 nutrients Nutrition 0.000 claims 1
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- 241000196324 Embryophyta Species 0.000 abstract description 17
- 238000009395 breeding Methods 0.000 abstract description 7
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- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 11
- 240000003768 Solanum lycopersicum Species 0.000 description 11
- 241000219146 Gossypium Species 0.000 description 8
- 230000002265 prevention Effects 0.000 description 3
- 241000317929 Chalcididae Species 0.000 description 2
- 240000000249 Morus alba Species 0.000 description 2
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- 244000046052 Phaseolus vulgaris Species 0.000 description 2
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000024241 parasitism Effects 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 241001073105 Aenasius bambawalei Species 0.000 description 1
- 235000007516 Chrysanthemum Nutrition 0.000 description 1
- 244000189548 Chrysanthemum x morifolium Species 0.000 description 1
- 235000015001 Cucumis melo var inodorus Nutrition 0.000 description 1
- 240000002495 Cucumis melo var. inodorus Species 0.000 description 1
- 241000257303 Hymenoptera Species 0.000 description 1
- MKYBYDHXWVHEJW-UHFFFAOYSA-N N-[1-oxo-1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propan-2-yl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(C(C)NC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 MKYBYDHXWVHEJW-UHFFFAOYSA-N 0.000 description 1
- 241000819999 Nymphes Species 0.000 description 1
- 241000209270 Phenacoccus solenopsis Species 0.000 description 1
- 241001415279 Pseudococcidae Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 230000002113 chemopreventative effect Effects 0.000 description 1
- 235000012343 cottonseed oil Nutrition 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000032669 eclosion Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005281 excited state Effects 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Catching Or Destruction (AREA)
Abstract
The present invention relates to a kind of method for breeding of Ban Shi encyrtid, are bred with germination potato to Ban Shi encyrtid, can shorten previous materials time, in order to avoid occur the shortage of host plant material during test.A large amount of space is saved, i.e. same space can get a large amount of parasitic wasp.And easily picking deadlock a red-spotted lizard, in case insect-taking gives test bring error not in time after Ban Shi encyrtid sprouts wings.Especially suitable for needing research or the field adaptability of a large amount of parasitic wasps, quickly to control Japan silk floss mealybug.
Description
Technical field
The invention belongs to cultured insect technical fields, are related to a kind of method for breeding of Ban Shi encyrtid.
Background technique
Ban Shi encyrtid Aenasius bambawalei Hayat belongs to Hymenoptera Encytidae Encyrtidae, is Japan
The important parasite of continuous mealybug, is a kind of internal excited state, be found for the first time in India in 2008, is distributed mainly on
State, India and Pakistan.
Japan silk floss mealybug Phenacoccus solenopsis Tinsley belongs to Semiptera Hemiptera Pseudococcidae
Pseudococcidae is a kind of pest for seriously threatening the plants such as field crop, ornamental plants in garden, fruit tree and vegetables, former
Is produced from North America, is pernicious invasion pest widely distributed in a kind of world wide.The tender position of children of main harm plant, with it is female at
Worm and nymph sucking plant juice, influence plant strain growth, which can also secrete honeydew pollution blade, lead to dark mildew, drop
Low leaf photosynthesis function causes aggrieved plant strain growth slowly or stops, causing plant dead in flakes when serious.For helping
The prevention and treatment of mulberry silk floss mealybug, physical control and chemopreventive effects are undesirable, and chemical prevention can bring " 3R " problem, therefore
Biological control is one of most effective and optimal method.In nature, the natural enemy of Japan silk floss mealybug is more, and Zhong Banshi is jumped
Chalcid fly is its Dominant Natural Enemies, is played an important role in the natural contral of Japan silk floss mealybug population, the biological control to the pest
Significant effect.
For the biological characteristics of Ban Shi encyrtid, reproductive development, to a series of research such as the prevention and treatment of Japan silk floss mealybug
It is very urgent.And it was studying into the middle insect for needing a large amount of physiological standards standardized.Therefore indoors artificial continuously rearing,
A large amount of parasitic wasps are obtained, are the premises for carrying out every research.Currently, mainly being helped for the host raised in Ban Shi encyrtid room
The mealybug on mealybug, cotton seedling, the mealybug on tomato seedling, the mealybug on potato seedling on mulberry.But Japan, cotton, tomato, soil
It is both needed to periodically water when bean seedlings are cultivated indoors, it is easily withered or even dead if watering is not in time.Potato seedling summer easily
Withered death.Simultaneously during watering, Ban Shi encyrtid easily escapes, and reduces feeding efficiency.Xiaoshan, Zhejiang linen-cotton is ground
Study carefully applied patent of invention " it is a kind of interior breeding mealybug and Ban Shi encyrtid method ", application No. is
201711286885.6, using Japan silk floss mealybug is connect on water planting radix achyranthis bidentatae chrysanthemum, carry out the breeding of Ban Shi encyrtid.The method is suitble to class
The foundation and maintenance of population in family name encyrtid room, but it is not suitable for a large amount of Ban Shi encyrtids of short time acquisition, as research material
Material or field adaptability Ban Shi encyrtid control Japan silk floss mealybug.Obtain a large amount of Ban Shi encyrtid in short-term, need it is easy to operate,
The easy method for breeding of worm is adopted, the present invention has such advantage.
Summary of the invention
A kind of the object of the present invention is to provide steps simple, a large amount of Ban Shi encyrtids of quick obtaining indoor continuously rearing sides
Method.
To achieve the goals above, technical scheme is as follows.
A kind of indoor continuously rearing method of Ban Shi encyrtid, comprising the following steps:
S1, the potato of weight 100g is nuzzled in wet sand, away from sand bed surface 2cm at the top of potato, waits its germination, etc.
Bud is long to 2cm, and it is spare that band bud potato is taken out in sand；
S2,25 Japan silk floss mealybug female adult pests or tritonymph are transferred on germination potato；
S3, germination potato is put into specification as 22cm × 15cm × 8cm together with Japan silk floss mealybug female adult pest or tritonymph
Insect box in, and place it on the feeding worm frame in insectary and raised, rearing conditions are as follows: 28 ± 2 DEG C of temperature, the photoperiod
14L:10D, relative humidity 80 ± 5%；
S4, be put into insect box sprout wings after Ban Shi encyrtid queen bee 4, the drone 2 that do not mate, allow its parasitic Japan
Continuous mealybug 72h holds the absorbent cotton for dipping in full 10% hydromel with the culture dish of diameter 3cm, for Ban Shi encyrtid adult Replacement Battalion
It supports；
After S5,72h, Ban Shi encyrtid is removed, these Ban Shi encyrtids continue the mealybug in other parasitic dependent insect cages, until
It is dead；
S6, continued to raise with germination potato by parasitic Japan silk floss mealybug, it, will be continuous by parasitic Japan after 8 days parasitic
Mealybug picks out, and is placed in 2ml centrifuge tube, waits its emergence, and 8:00 every morning and evening 20:00 are respectively observed once, note
Record quantity, emergence time and the male and female of emergence parasitic wasp；The parasitic wasp of emergence is for studying, field adaptability or raising up seed；
S7, step S1-S6 is repeated, continuously rearing is carried out to Ban Shi encyrtid.
Preferentially, in step sl, potato is embedded in wet sand, every 2d, is sprayed water to sand, make its guarantor
It holds wet.
Preferentially, in step s 2,25 Japan silk floss mealybug female adult pests or tritonymph are shifted on a germination potato, are protected
Holding germination potato has suitable Japan silk floss mealybug quantity.The Japan silk floss mealybug body size shifted is uniform, guarantees that Ban Shi is jumped
Small bee imago size is uniform.
Preferentially, in step s3, two germination potatoes are one group, and it is 22cm that totally 50 Japan silk floss mealybugs, which are placed on specification,
In the insect box of × 15cm × 8cm, sealed at the top of insect box with 100 mesh gauzes.
Preferentially, in step s3, insectary, which is placed, supports worm frame, and the specification for supporting worm frame is 110cm × 50cm × 200cm,
Each feeding worm frame has 4 layers, 50cm every layer high, and the top of every layer frame is equipped with fluorescent tube, as light source.The temperature of insectary passes through sky
Regulation system, relative humidity are controlled by humidifier, and light irradiation time is by intelligent switching control, and it is real-time to place Hygrothermograph progress
Monitoring.
Preferentially, in step s 6, it will be individually placed in centrifuge tube by parasitic Japan silk floss mealybug, facilitate and observe its plumage
Change, in this way it is easy to determine the range of emergence time, and ensuring to test Ban Shi encyrtid used is not mate after sprouting wings.Every morning
8:00 and evening 20:00 is respectively observed once, records quantity, emergence time and the male and female of emergence parasitic wasp.Ban Shi after emergence is jumped
Chalcid fly further to raise, scientific research or field adaptability.
Invention has the beneficial effects that: the present invention breeds Ban Shi encyrtid with germination potato, can shorten early period
Material time, in order to avoid occur the shortage of host plant material during test.Save a large amount of space, i.e. same space
It can get a large amount of parasitic wasp.And easily picking deadlock a red-spotted lizard, in case insect-taking gives test bring not in time after Ban Shi encyrtid sprouts wings
Error.Especially suitable for needing research or the field adaptability of a large amount of parasitic wasps, quickly to control Japan silk floss mealybug.In the invention,
Potato easily germinates, and can directly buy the potato of germination on the market, rots within the potato of germination general 2 months or so.And class
Time of the family name encyrtid from parasitism to emerging adult is 15d or so.The potato of one germination can be used 3 times.And potato volume
Small, required space is few, in identical space, can put more insect box, raises more Ban Shi encyrtids.By parasitic Japan
On potato surface, facilitating will be picked out continuous mealybug by parasitic Japan silk floss mealybug.The indoor subculture of above-mentioned Ban Shi encyrtid is raised
The method of supporting is easy to operate, greatly improves feeding worm efficiency, can obtain a large amount of parasitic wasp the short time to study or field is released
It puts.
Specific embodiment
A specific embodiment of the invention is described below with reference to embodiment, to better understand the present invention.
Embodiment 1
The indoor feeding method of one of the present embodiment Ban Shi encyrtid, host plant are potato bud, specific steps
It is as follows:
S1, the potato for weighing about 100g is nuzzled in wet sand, away from sand bed surface about 2cm at the top of potato, is germinateed to it,
It is long to about 2cm to bud, it is spare that band bud potato is taken out in sand；
S2,25 Japan silk floss mealybug female adult pests or tritonymph are transferred on germination potato；
S3, germination potato is put into specification as 22cm × 15cm × 8cm together with Japan silk floss mealybug female adult pest or tritonymph
Insect box in, and place it on the feeding worm frame in insectary and raised, support worm frame specification be 110cm × 50cm ×
200cm, each feeding worm frame divides four layers, therefore a feeding worm frame in insectary can place 48 insect box, rearing conditions are as follows: temperature
28 ± 2 DEG C, photoperiod 14L:10D of degree, relative humidity 80 ± 5%；
S4, be put into insect box sprout wings after Ban Shi encyrtid queen bee 4, the drone 2 that do not mate, allow its parasitic Japan
Continuous mealybug 72h holds the absorbent cotton for dipping in full 10% hydromel with the culture dish of diameter 3cm, for Ban Shi encyrtid adult Replacement Battalion
It supports；
After S5,72h, Ban Shi encyrtid is removed, these Ban Shi encyrtids continue the mealybug in other parasitic dependent insect cages, until
It is dead.
S6, continued to raise with germination potato by parasitic Japan silk floss mealybug, it, will be continuous by parasitic Japan after 8 days parasitic
Mealybug picks out, and is placed in 2ml centrifuge tube, waits its emergence, and 8:00 every morning and evening 20:00 are respectively observed once, note
Record quantity, emergence time and the male and female of emergence parasitic wasp.The parasitic wasp of emergence is for studying, field adaptability or raising up seed；
S7, step S1-S6 is repeated, continuously rearing is carried out to Ban Shi encyrtid.
Comparative example 1
The indoor feeding method of Ban Shi encyrtid in this comparative example, host plant are potato seedling, the specific steps of which are as follows:
S1, the potato for weighing about 100g is nuzzled in wet sand, away from sand bed surface about 2cm at the top of potato, waits its hair
Bud；
S2, etc. buds it is long to 2cm or so, from potato is taken out in sand by its kind in the flowerpot of 10cm × 10cm, wait potatoes
Miao Changzhi 25cm is spare, during which periodically to water, and dies in order to avoid potato seedling stem is withered；
S3,50 Japan silk floss mealybug female adult pests or tritonymph are shifted on the potato seedling of supreme 25cm；
S4, potato seedling is put into dependent insect cage and is raised, the specification of dependent insect cage is 20cm × 20cm × 30cm band sleeve
Sarong, and periodically water from cuff, dependent insect cage is placed on the feeding worm frame of insectary and is raised, the specification for supporting worm frame is
110cm × 50cm × 200cm, each feeding worm frame divides four layers, therefore a feeding worm frame in insectary can place 20 dependent insect cages,
Rearing conditions are as follows: 28 ± 2 DEG C of temperature, photoperiod 14L:10D, relative humidity 80 ± 5%；
S5, be put into dependent insect cage sprout wings after Ban Shi encyrtid queen bee 4, the drone 2 that do not mate, allow its parasitic Japan
Continuous mealybug 72h holds the absorbent cotton for dipping in full 10% hydromel with the culture dish of diameter 3cm, for Ban Shi encyrtid adult Replacement Battalion
It supports；
After S6,72h, Ban Shi encyrtid is removed, these Ban Shi encyrtids continue the mealybug in other parasitic dependent insect cages, until
It is dead.
S7, continued to raise with potato seedling by parasitic Japan silk floss mealybug, it, will be by parasitic Japan's silk floss powder after 8 days parasitic
A red-spotted lizard picks out, and is placed in 2ml centrifuge tube, waits its emergence, and 8:00 every morning and evening 20:00 are respectively observed once, record
Quantity, emergence time and the male and female of emergence parasitic wasp.The parasitic wasp of emergence is for studying, field adaptability or raising up seed；
S8, step S1-S7 is repeated, continuously rearing is carried out to Ban Shi encyrtid.
Comparative example 2
The indoor feeding method of Ban Shi encyrtid in this comparative example, host plant are tomato seedling, the specific steps of which are as follows:
S1, tomato seeds are sprinkling upon in seedlings nursing plate, carry out nursery；
S2, by the tomato transplantation of seedlings of high 10cm in the flowerpot of 10cm × 10cm, wait tomato seedlings length it is spare to 25cm, during which
It periodically to water；
S3,50 Japan silk floss mealybug female adult pests or tritonymph are shifted on the tomato seedling of supreme 25cm；
S4, tomato seedling is put into dependent insect cage and is raised, the specification of dependent insect cage is 20cm × 20cm × 30cm band sleeve
Sarong, and periodically water from cuff, dependent insect cage is placed on the feeding worm frame of insectary and is raised, the specification for supporting worm frame is
110cm × 50cm × 200cm, each feeding worm frame divides four layers, therefore a feeding worm frame in insectary can place 20 dependent insect cages,
Rearing conditions are as follows: 28 ± 2 DEG C of temperature, photoperiod 14L:10D, relative humidity 80 ± 5%；
S5, be put into dependent insect cage sprout wings after Ban Shi encyrtid queen bee 4, the drone 2 that do not mate, allow its parasitic Japan
Continuous mealybug 72h holds the absorbent cotton for dipping in full 10% hydromel with the culture dish of diameter 3cm, for Ban Shi encyrtid adult Replacement Battalion
It supports；
After S6,72h, Ban Shi encyrtid is removed, these Ban Shi encyrtids continue the mealybug in other parasitic dependent insect cages, until
It is dead.
S7, continued to raise with tomato seedling by parasitic Japan silk floss mealybug, it, will be by parasitic Japan's silk floss powder after 8 days parasitic
A red-spotted lizard picks out, and is placed in 2ml centrifuge tube, waits its emergence, and 8:00 every morning and evening 20:00 are respectively observed once, record
Quantity, emergence time and the male and female of emergence parasitic wasp.The parasitic wasp of emergence is for studying, field adaptability or raising up seed；
S8, step S1-S7 is repeated, continuously rearing is carried out to Ban Shi encyrtid.
Comparative example 3
The indoor feeding method of Ban Shi encyrtid in this comparative example, host plant are cotton seedling, the specific steps of which are as follows:
S1, cotton seeds are sowed in the flowerpot of 10cm × 10cm, waits cotton seedlings length spare to 25cm, it is during which regular
It waters；
S2,50 Japan silk floss mealybug female adult pests or tritonymph are shifted on the cotton seedling of supreme 25cm；
S3, cotton seedling is put into dependent insect cage and is raised, the specification of dependent insect cage is 20cm × 20cm × 30cm band sleeve
Sarong, and periodically water from cuff, dependent insect cage is placed on the feeding worm frame of insectary and is raised, the specification for supporting worm frame is
110cm × 50cm × 200cm, each feeding worm frame divides four layers, therefore a feeding worm frame in insectary can place 20 dependent insect cages,
Rearing conditions are as follows: 28 ± 2 DEG C of temperature, photoperiod 14L:10D, relative humidity 80 ± 5%；
S4, be put into dependent insect cage sprout wings after Ban Shi encyrtid queen bee 4, the drone 2 that do not mate, allow its parasitic Japan
Continuous mealybug 72h holds the absorbent cotton for dipping in full 10% hydromel with the culture dish of diameter 3cm, for Ban Shi encyrtid adult Replacement Battalion
It supports；
After S5,72h, Ban Shi encyrtid is removed, these Ban Shi encyrtids continue the mealybug in other parasitic dependent insect cages, until
It is dead.
S6, continued to raise with cotton seedling by parasitic Japan silk floss mealybug, it, will be by parasitic Japan's silk floss powder after 8 days parasitic
A red-spotted lizard picks out, and is placed in 2ml centrifuge tube, waits its emergence, and 8:00 every morning and evening 20:00 are respectively observed once, record
Quantity, emergence time and the male and female of emergence parasitic wasp.The parasitic wasp of emergence is for studying, field adaptability or raising up seed；
S7, step S1-S7 is repeated, continuously rearing is carried out to Ban Shi encyrtid.
Comparative example 4
The indoor feeding method of Ban Shi encyrtid in this comparative example, host plant are Japan, the specific steps of which are as follows:
S1, Japan's seedling is bought on the market, it is spare when its length is to 25cm high；
S2,50 Japan silk floss mealybug female adult pests or tritonymph are shifted in Japan of supreme 25cm；
S3, Japan is put into dependent insect cage and is raised, the specification of dependent insect cage is 20cm × 20cm × 30cm band sleeve yarn
Cage, and periodically water from cuff, dependent insect cage is placed on the feeding worm frame of insectary and is raised, the specification for supporting worm frame is
110cm × 50cm × 200cm, each feeding worm frame divides four layers, therefore a feeding worm frame in insectary can place 20 dependent insect cages,
Rearing conditions are as follows: 28 ± 2 DEG C of temperature, photoperiod 14L:10D, relative humidity 80 ± 5%；
S4, be put into dependent insect cage sprout wings after Ban Shi encyrtid queen bee 4, the drone 2 that do not mate, allow its parasitic Japan
Continuous mealybug 72h holds the absorbent cotton for dipping in full 10% hydromel with the culture dish of diameter 3cm, for Ban Shi encyrtid adult Replacement Battalion
It supports；
After S5,72h, Ban Shi encyrtid is removed, these Ban Shi encyrtids continue the mealybug in other parasitic dependent insect cages, until
It is dead.
S6, continued to raise with Japan by parasitic Japan silk floss mealybug, it, will be by parasitic Japan silk floss mealybug after 8 days parasitic
It picks out, is placed in 2ml centrifuge tube, wait its emergence, and 8:00 every morning and evening 20:00 are respectively observed once, record plumage
Change quantity, emergence time and the male and female of parasitic wasp.The parasitic wasp of emergence is for studying, field adaptability or raising up seed；
S7, step S1-S6 is repeated, continuously rearing is carried out to Ban Shi encyrtid.
1 embodiment 1 of table and comparative example 1-4 Comparative result
| Embodiment 1 | Comparative example 1 | Comparative example 2 | Comparative example 3 | Comparative example 4 | |
| The time required to connecing before worm (d) | 10 | 25 | 60 | 25 | 15 |
| Period watering | It is no | It is | It is | It is | It is |
| Dependent insect cage specification (cm) | 22×15×8 | 20×20×30 | 20×20×30 | 20×20×30 | 20×20×30 |
| Support the put dependent insect cage quantity of worm frame | 48 | 20 | 20 | 20 | 20 |
| Connect mealybug difficulty or ease | Easily | It is difficult | It is difficult | It is easier to | It is more difficult |
| Stiff a red-spotted lizard picking difficulty or ease | Easily | It is difficult | It is more difficult | It is difficult | It is more difficult |
| Parasitic rate | 81.96% | 76.94% | 60.43% | 72.24% | 70.43% |
| Sex ration | 1.22:1 | 1.15:1 | 1.62:1 | 1.33:1 | 1.28:1 |
As seen from Table 1:
Potato needs 10d to the bud for growing 2cm or so, can carry out connecing worm experiment after 10d, tomato is from seed to height
The seedling of 25cm or so needs 60d, and cotton needs 25d, potato Miao Ze to need 25d, and Japan needs 15d.Therefore, it germinates potato early period
Time is most short, and it is spare to be once ready for a large amount of potato.Or can directly buy germination potato carry out Ban Shi jump it is small
The breeding of bee.
In all hosts, in addition to germination potato does not need watering, remaining four kinds of host is both needed to regular watering.In
In watering proce, it is be easy to cause the escape of Ban Shi encyrtid, reduces the efficiency of feeding worm.
In all hosts, dependent insect cage specification needed for the potato of germination is minimum, therefore, a shelf in insectary
In upper, the potato of germination can put 48 insect box, remaining puts 20 dependent insect cages.Therefore in identical space, the soil of germination
Beans can get more parasitic wasp individuals, improve feeding worm efficiency.
Mealybug is placed directly in potato surface by the difficulty or ease for connecing worm, and mealybug is easy to slide and is easy to reappose, and connects worm in seedling
On, need to consider the smooth degree of blade surface, the surface area of blade, need one by one worm is placed on blade surface.And powder
A red-spotted lizard is not easy to be placed again into surface, reduces efficiency after blade landing.
The difficulty or ease of stiff a red-spotted lizard picking will be come out, picking goes out after Japan silk floss mealybug was by parasitism 8 days by parasitic stiff a red-spotted lizard picking
The stiff a red-spotted lizard come is individually placed in 2ml centrifuge tube, is waited and being sprouted wings.Picking after sprouting wings is avoided to influence subsequent experimental result not in time.
Because stiff a red-spotted lizard is on germination potato surface, and stiff a red-spotted lizard compares concentration, and easily picking comes out.And stiff a red-spotted lizard is on other four kinds of host plants
Position are as follows: blade surface, vacuum side of blade, in stalk, it is more dispersed, be not easy picking, and frangibility blade during picking.
Because of the difference of host plant, field adaptability also has certain difference, entire germination potato can be put into field, etc.
To parasitic bee eclosion, and remaining four kinds of host plant can only win blade and be put into the emergence that field waits parasitic wasp.Blade compares
Gently, then the easy influence by weather such as blowing and rainings in field is put, and excised leaf is not easy to maintain.
Host is different, the also difference of the parasitic rate under the same terms.Wherein, the parasitic rate highest on germination potato,
It is 81.96%.The parasitic rate of tomato seedling is minimum, is 60.43%.
Host is different, and the sex ration of produced offspring is also different.Experiment shows the sex ration of offspring produced by several hosts
It is not much different.In 1.2:1 or so.
By comparing discovery above, the breeding of Ban Shi encyrtid is carried out using germination potato, can be saved largely before connecing worm
Time.It is identical to connect worm amount and after connecing worm, a large amount of space can be saved.Potato easily germinates, easy to maintain after germination, and not vulnerable to
To the influence of weather, influence of the weather to plant growth speed is avoided.Therefore Ban Shi encyrtid is carried out with the potato of germination
Successive propagation have it is easy to operate, the short time obtains the advantage of the consistent parasitic wasp of a large amount of standards.
The above is a preferred embodiment of the present invention, it is noted that for those skilled in the art
For, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also considered as
Protection scope of the present invention.
Claims (6)
1. a kind of indoor continuously rearing method of Ban Shi encyrtid, it is characterised in that: the following steps are included:
S1, the potato of weight 100g is nuzzled in wet sand, away from sand bed surface 2cm at the top of potato, waits its germination, wait buds long
To 2cm, it is spare that band bud potato is taken out in sand；
S2,25 Japan silk floss mealybug female adult pests or tritonymph are transferred on germination potato；
S3, the potato that will germinate are put specification into together with Japan silk floss mealybug female adult pest or tritonymph and are supported for 22cm × 15cm × 8cm
In worm box, and places it on the feeding worm frame in insectary and raised, rearing conditions are as follows: 28 ± 2 DEG C of temperature, photoperiod 14L:
10D, relative humidity 80 ± 5%；
S4, be put into insect box sprout wings after Ban Shi encyrtid queen bee 4, the drone 2 that do not mate, allow its parasitic Japan silk floss powder
A red-spotted lizard 72h holds the absorbent cotton for dipping in full 10% hydromel with the culture dish of diameter 3cm, for Ban Shi encyrtid Adult supplement nutrient；
After S5,72h, Ban Shi encyrtid is removed, these Ban Shi encyrtids continue the mealybug in other parasitic dependent insect cages, until dead
It dies；
S6, continued to raise with germination potato by parasitic Japan silk floss mealybug, it, will be by parasitic Japan silk floss mealybug after 8 days parasitic
It picks out, is placed in 2ml centrifuge tube, wait its emergence, and 8:00 every morning and evening 20:00 are respectively observed once, record plumage
Change quantity, emergence time and the male and female of parasitic wasp；The parasitic wasp of emergence is for studying, field adaptability or raising up seed；
S7, step S1-S6 is repeated, continuously rearing is carried out to Ban Shi encyrtid.
2. a kind of indoor continuously rearing method of Ban Shi encyrtid according to claim 1, it is characterised in that: the step
In S1, potato is embedded in wet sand, every 2d, is sprayed water to sand, it is made to keep wet.
3. a kind of indoor continuously rearing method of Ban Shi encyrtid according to claim 1, it is characterised in that: the step
In S2,25 Japan silk floss mealybug female adult pests or tritonymph are shifted on a germination potato, keep germination potato to have suitable
Japan silk floss mealybug quantity；The Japan silk floss mealybug body size shifted is uniform, guarantees that Ban Shi encyrtid adult size is uniform.
4. a kind of indoor continuously rearing method of Ban Shi encyrtid according to claim 1, it is characterised in that: the step
In S3, two germination potatoes are one group, and totally 50 Japan silk floss mealybugs are placed on specification and are in 22cm × 15cm × 8cm insect box,
It is sealed at the top of insect box with 100 mesh gauzes.
5. a kind of indoor continuously rearing method of Ban Shi encyrtid according to claim 1, it is characterised in that: the step
In S3, insectary, which is placed, supports worm frame, and the specification for supporting worm frame is 110cm × 50cm × 200cm, and each feeding worm frame has 4 layers, every layer of height
The top of 50cm, every layer frame are equipped with fluorescent tube, as light source；The temperature of insectary is by airconditioning control, and relative humidity is by adding
Wet device control, light irradiation time are placed Hygrothermograph and are monitored in real time by intelligent switching control.
6. a kind of indoor continuously rearing method of Ban Shi encyrtid according to claim 1, it is characterised in that: the step
It in S6, will be individually placed in centrifuge tube by parasitic Japan silk floss mealybug, facilitate its emergence of observation, in this way it is easy to determine the model of emergence time
It encloses, and ensuring to test Ban Shi encyrtid used is not mate after sprouting wings；Every morning, 8:00 and evening 20:00 respectively observed one
It is secondary, record quantity, emergence time and the male and female of emergence parasitic wasp；Ban Shi encyrtid after emergence further to raise, science
Research or field adaptability.
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| CN113439715A (en) * | 2021-07-30 | 2021-09-28 | 浙江省农业科学院 | Indoor successive transfer breeding method of lycoris radiata mealybugs |
| CN113678790A (en) * | 2021-07-30 | 2021-11-23 | 浙江省农业科学院 | Method for optimizing Babylonia wasp population structure in host conversion mode |
| CN113951214A (en) * | 2021-12-01 | 2022-01-21 | 浙江省农业科学院 | Method for prolonging storage time of Brachypodium banseri and application thereof |
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