CN112753656A - Breeding device and indoor seed preservation method for Hemerocallis ventricosus such as natural enemy of sugarcane striped rice borer - Google Patents
Breeding device and indoor seed preservation method for Hemerocallis ventricosus such as natural enemy of sugarcane striped rice borer Download PDFInfo
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- 238000009395 breeding Methods 0.000 title claims abstract description 20
- 230000001488 breeding effect Effects 0.000 title claims abstract description 19
- 238000000034 method Methods 0.000 title claims abstract description 19
- 238000004321 preservation Methods 0.000 title claims description 24
- 241000756137 Hemerocallis Species 0.000 title description 3
- 235000013601 eggs Nutrition 0.000 claims abstract description 98
- 230000003071 parasitic effect Effects 0.000 claims abstract description 85
- 238000012360 testing method Methods 0.000 claims abstract description 47
- 229920000742 Cotton Polymers 0.000 claims abstract description 45
- 238000011081 inoculation Methods 0.000 claims abstract description 27
- 210000004681 ovum Anatomy 0.000 claims abstract description 26
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 17
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 17
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- 210000001015 abdomen Anatomy 0.000 claims description 28
- 241000257303 Hymenoptera Species 0.000 claims description 17
- 238000002347 injection Methods 0.000 claims description 12
- 239000007924 injection Substances 0.000 claims description 12
- 230000003187 abdominal effect Effects 0.000 claims description 8
- 238000010521 absorption reaction Methods 0.000 claims description 8
- 235000012907 honey Nutrition 0.000 claims description 7
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- 241000256618 Trichogramma Species 0.000 claims description 3
- 238000009423 ventilation Methods 0.000 claims description 2
- 108010058846 Ovalbumin Proteins 0.000 claims 5
- 229940092253 ovalbumin Drugs 0.000 claims 5
- 230000000384 rearing effect Effects 0.000 claims 1
- 238000011161 development Methods 0.000 abstract description 10
- 230000002035 prolonged effect Effects 0.000 abstract description 2
- 238000001035 drying Methods 0.000 abstract 1
- 230000032669 eclosion Effects 0.000 description 10
- 230000018109 developmental process Effects 0.000 description 9
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- 238000013461 design Methods 0.000 description 3
- 241000719836 Anoectochilus formosanus Species 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- 241001481304 Vespoidea Species 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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Abstract
The invention discloses a feeding device and an indoor seed protection method for black ovalbumus ventricosus such as natural enemy of sugarcane striped rice borer, comprising a device body, wherein the device body is provided with a first feeding chamber and a second feeding chamber which are separated by a movable baffle; a plurality of groups of parasitic egg placing frames are arranged in the first feeding chamber and the second feeding chamber; cotton is arranged on the parasitic ovum placing rack; test tube racks are respectively arranged on two side walls, adjacent to the second feeding chamber, in the first feeding chamber; an opening with a door leaf is arranged on the side wall of one side of the first feeding chamber opposite to the second feeding chamber; the tops of the first feeding chamber and the second feeding chamber are provided with openable cover bodies; the first feeding chamber and the second feeding chamber are respectively provided with a temperature and humidity controller. The device of the application can be used for continuously breeding simultaneously, each stage does not influence each other, the practicability is strong, the efficiency is high, the space is saved simultaneously, the damage of eggs of the striped rice borers or the drying and shrinkage of the eggs can be effectively reduced, the inoculation parasitic rate and the emergence rate are improved, and the development duration and the indoor longer-term storage are prolonged.
Description
Technical Field
The invention relates to the technical field of agricultural pest control, in particular to a feeding device and an indoor seed preservation method for black oophagous ventriculi, such as natural enemies of sugarcane striped rice borers.
Background
The sugarcane striped rice borer is an important borer pest which is harmful to sugarcane, mainly takes the fact that larvae eat sugarcane seedlings and sugarcane stalks to cause damage, damages are caused to dead seedlings in seedling stage, damages are caused to tip withered (dead tail) or borer joints after elongation and jointing, and has great influence on the yield and sugar content of the sugarcane. The anoplophora ventricosa is a dominant egg parasitic natural enemy of the sugarcane striped rice borer, can control the generation of the anoplophora ventricosa in the egg stage, and can effectively prevent the damage of larvae to sugarcane. The host specialization of the anopheles ventricosa is strong, no intermediate host which is easy to propagate the anopheles indoors in a large quantity exists at present, and the research, development and application of the anopheles ventricosa are limited because the generation cycle of the target host sugarcane striped rice borer is long and the target host sugarcane striped rice borer is easy to degenerate after being continuously raised for multiple generations. Under the condition of 26 degrees, more than 40 days are needed for 1 generation of the striped rice borer, about 11 days are needed for the black bee in the abdomen to complete one generation, and about 10 days can survive the black bee in the abdomen under the honey feeding condition. The black-egg bees in the abdomen can complete development under the condition of 17-32 ℃, but the emergence rate is extremely low under the condition of less than 14 ℃; the survival rate of the adult anophelifuge wasps is high under the low-temperature condition of 10-14 ℃. In the case of a shortage of eggs of the striped rice borer, the conservation of the breed of the black-egg wasp such as the natural enemy thereof is extremely important. In order to better breed, research and apply the black wasps, an indoor preservation method for carrying out different treatments on different insect states is very critical.
In the current research, a device suitable for inoculation of the egg parasitic wasps has been developed, but the device is only suitable for inoculation and experimental observation, but is not suitable for effective preservation of eggs, larvae and pupae after inoculation, so that the design of a device for indoor breeding and preservation of the black abdominal wasps and a method for preserving the black abdominal wasps by performing different treatments on different insect states are also necessary.
Disclosure of Invention
The invention provides a feeding device and an indoor seed protection method for black-egg bees such as sugarcane striped rice borer natural enemies, and aims to solve the technical problems that the device for inoculating the egg parasitic wasps is only suitable for inoculation and experimental observation, but is not suitable for egg, larva and pupal period storage after inoculation.
In order to solve the problems, the invention adopts the following technical scheme:
device is raised to black egg bee such as sugarcane striped rice borer natural enemy includes:
the device comprises a device body, wherein a first feeding chamber and a second feeding chamber are arranged in the device body; the first feeding chamber and the second feeding chamber are separated by a movable baffle plate;
a plurality of groups of parasitic egg placing frames are respectively arranged in the first feeding chamber and the second feeding chamber; cotton is arranged on the parasitic ovum placing rack;
test tube racks are respectively arranged on two side walls, adjacent to the second feeding chamber, in the first feeding chamber;
an opening with a door leaf is arranged on the side wall of one side of the first feeding chamber opposite to the second feeding chamber; the tops of the first feeding chamber and the second feeding chamber are provided with openable cover bodies; the first feeding chamber and the second feeding chamber are respectively provided with a temperature and humidity controller.
Further, the test-tube rack is vertically arranged in the feeding chamber, and a plurality of accommodating chambers for accommodating test tubes are arranged on the test-tube rack.
Furthermore, a plurality of groups of sliding rails are arranged at the bottom of the device body from the first feeding chamber to the second feeding chamber; the plurality of groups of parasitic ovum placing racks are slidably arranged on the sliding rail; the baffle plate is provided with an opening at a position corresponding to the slide rail, so that the end part of the baffle plate can be completely abutted against the bottom of the device body.
Further, the parasitic placing frame comprises a fixing block and two clamping pieces; the fixed block is slidably arranged on the slide rail, and the two clamping pieces are fixed on the fixing block and used for clamping the blade containing the parasitic ovum; the cotton is arranged on the opposite inner sides of the two clamping pieces.
Further, a water storage chamber is arranged in the fixed block; the fixed block is provided with a water injection hole and a water diversion hole, and the water injection hole and the water diversion hole are respectively communicated with the water storage chamber; the water diversion hole is internally provided with water absorption cotton, one end of the water absorption cotton extends and is soaked in the water storage chamber, and the other end of the water absorption cotton is connected with the cotton.
Furthermore, the device body is a transparent box body.
Furthermore, a breathable net is arranged on the water injection hole in an opening and closing manner.
Another object of the present invention is to provide a method for preserving indoor species of black-egg bees in iso-abdominal cavity by using the above breeding apparatus, which comprises the steps of:
(1) inoculation and propagation: after cotton on a parasitic egg placing frame in a first feeding room absorbs water, placing fresh and plump striped rice borer egg blocks on the parasitic egg placing frame, adjusting the temperature in the first feeding room to be 26-28 ℃, adjusting the air humidity to be 75-85%, placing a test tube filled with anoplophora ventricosa on a test tube rack in the first feeding room, and carrying out parasitic operation for 20-30 hours;
(2) egg, larva and pupal stage preservation: transferring the parasitic striped rice borer egg blocks and the parasitic egg placing rack to a second feeding room; adjusting the temperature of the second feeding room to be 17-20 ℃ through a temperature and humidity controller, keeping the cotton on the parasitic egg placing rack moist after the humidity is 80%, and taking out the cotton after the cotton is parasitic until 1-2 days before the black oophagous wasps in the abdomen emerge;
(3) and (4) adult-period preservation: taking out parasitic ovum, moistening with cotton, placing in a large test tube with 30% Mel water coated on tube wall, placing the large test tube on a test tube rack of a first feeding room until bee eclosion; after the black oophagous trichogrammae emerges and fully mates for 6-8 hours, introducing the female trichogramma of the black oophagous trichogrammae into a fingering tube for split charging, and plugging the tube opening with cotton; transferring the finger-shaped tube filled with the black oophagous wasps to a low-temperature incubator for storage.
Wherein, in the first feeding room, the ratio of the number of the female bees of the black-egg bees to the number of the eggs of the striped rice borers is 1: 15-20.
Wherein, in the step (3), the preservation condition of the finger-shaped tube filled with the anoectochilus formosanus is that the temperature is 10 ℃, and the air humidity is 75%.
Compared with the prior art, the invention has the following beneficial effects:
(1) and this application device raises the room through at this internal first room and the second of raising of setting up of device, first raising the room and being used for the inoculation and the mating after eclosion, the room is raised to the second is used for preserving the striped rice borer ovum after the inoculation, this application device can go on simultaneously and go on in succession, each stage does not influence each other, if first raising indoor, can continue inoculation breed after transferring the striped rice borer ovum that parasitizes to the second raising room, and the eclosion mating stage does not influence the preservation of adult period ovum in the room is raised to the second yet, therefore, the practicality of the invention is strong, high efficiency, save space simultaneously, compare in prior art each device of breeding stage with different, the striped rice borer ovum transfer after this application is posted is more convenient, and is safer, can effectively reduce striped rice borer ovum damage or loss of water and dry and shriveled, show the success rate that improves inoculation and breed. The device is also suitable for feeding and controlling other bees, such as trichogramma.
(2) According to the method, the temperature and the humidity of the environment in the inoculation period are controlled to be 26-28 ℃, the air humidity is 75-85%, the parasitic rate is highest, the temperature and the humidity for storing the black oophagous wasps in the egg, larva and pupa periods are controlled to be 17-20 ℃, the humidity is 80%, the growth rate of the black oophagous wasps is lowest when the black oophagous wasps peak is 10 ℃, the air humidity is 75%, the growth period is longest, and the problems that the black oophagous wasps in the abdomen are lack and difficult to store in winter are effectively solved. On the basis, the device of the application is used for inoculation, propagation and preservation, and the parasitic rate and the emergence rate of the inoculation and propagation are both improved.
Drawings
FIG. 1 is a schematic structural view of a feeding device for Hemerocallis ventricosus, such as a natural enemy of sugarcane striped rice borer, according to the invention;
FIG. 2 is an enlarged view of a portion of FIG. 1 at A;
in the figure, 1-a device body, 2-a first feeding chamber, 3-a second feeding chamber, 4-a baffle, 5-a parasitic ovum placing rack 51-cotton, 52-a fixed block, 53-a water injection hole, 54-absorbent cotton, 6-a test tube rack, 61-a containing cavity, 7-an opening, 8-a cover body, 9-a temperature and humidity controller and 10-a slide rail.
Detailed Description
The present invention will be further described with reference to examples and tests.
Example 1
As shown in fig. 1-2, the device for raising the black bee abdominal shell, such as the natural enemy of the sugarcane striped rice borer, comprises:
the device comprises a device body 1, wherein a first feeding chamber 2 and a second feeding chamber 3 are arranged in the device body 1, and the first feeding chamber 2 and the second feeding chamber 3 are separated by a movable baffle plate;
a plurality of groups of parasitic egg placing frames 5 are respectively arranged in the first feeding chamber 2 and the second feeding chamber 3; cotton 51 is arranged on the parasitic ovum placing frame 5;
Preferably, in the present embodiment, the test tube rack 6 is vertically disposed in the feeding chamber, and the test tube rack 6 is provided with a plurality of cavities 61 for holding test tubes. The test tube rack 6 is used for placing parasitic eggs to be eclosized after being coated with honey and placing test tubes filled with black-egg bees in the abdomen.
Preferably, in the present embodiment, the bottom of the apparatus body 1 is further provided with a plurality of sets of slide rails 10 from the first feeding chamber 2 to the second feeding chamber 3; a plurality of groups of parasitic ovum placing racks 5 are slidably arranged on the sliding rail 10. The baffle is provided with an opening at a position corresponding to the slide rail 10, so that the end part of the baffle can be completely abutted against the bottom of the device body 1. The parasitic placing frame comprises a fixing block 52 and two clamping pieces; the fixed block 52 is slidably arranged on the slide rail 10, and the two clamping pieces are fixed on the fixed block and used for clamping the blade containing the parasitic ovum; the cotton 51 is disposed on the opposite inner sides of the two grippers. When inoculation is completed, parasitic eggs after being parasitic need to be transferred to the second feeding chamber 3, the parasitic eggs can be directly slid into the second feeding chamber 3 together with the parasitic egg placing frame 5 to be stored and eclosized under the condition that the parasitic eggs are not moved by the aid of the slide rail 10, damage or falling of the parasitic eggs in the moving process is reduced, and the storage effect is further improved. During transferring, the sliding baffle 4 communicates the first feeding chamber 2 with the second feeding chamber 3, and then the parasitic egg placing frame 5 slides to the second feeding chamber 3, so that the parasitic egg transferring is completed. Of course, in other embodiments, the slide rails 10 are not designed, and the cover bodies 8 on the first feeding chamber 2 and the second feeding chamber 3 may be opened directly, taken out directly from the cover body 8 on the first feeding chamber 2, and put into the second feeding chamber 3 from the cover body 8 on the second feeding chamber 3 for storage, and this embodiment may also realize effective transfer of the parasitic eggs.
Preferably, in the present embodiment, a water storage chamber is provided in the fixed block 52, a water injection hole 53 and a water diversion hole are provided in the fixed block 52, the water injection hole 53 and the water diversion hole are respectively communicated with the water storage chamber, a water absorption cotton 54 is provided in the water diversion hole, one end of the water absorption cotton 54 extends and is soaked in the water storage chamber, and the other end is connected with the cotton 51. Parasitic eggs of the black-egg bees are usually striped rice borer egg masses which are generally adhered to plant leaves such as sugarcane leaves and rice leaves, and in order to prevent the withered plant leaves and influence the storage quality of the parasitic eggs, moisture contained in a water storage chamber in the fixing block 52 is attracted to the cotton 51 through the absorbent cotton 54, so that the cotton 51 is kept moist, and the quality of the plant leaves and the parasitic eggs is further ensured.
Preferably, in the present embodiment, the device body 1 is a transparent box body, which facilitates observation of the black bee abdominal shell inoculation and the development after inoculation.
Preferably, in the present embodiment, a ventilation net is provided to the water injection holes 53 so as to be opened and closed. When water is needed to be injected into the fixing block 52, the breathable net is opened to inject water, and after the water injection is completed, the breathable net is closed, so that the phenomenon that the wings of the anoplophora aurantiaca are stuck to water drops on the water injection holes 53 and fall into the water storage chamber can be prevented.
In the present application, the temperature and humidity controller 9 may be any one of the controllers capable of displaying temperature and humidity control in the prior art, such as but not limited to a digital display temperature and humidity controller 9 disclosed in patent application No. CN 202020293841.7.
Of course, in other embodiments, the design of the test tube rack 6 can refer to the design of the test tube rack in the patent CN207897735U previously applied by the applicant.
The application device has the following use processes:
firstly, a baffle plate is slid to separate a first feeding chamber 2 from a second feeding chamber 3, a ventilating net on a fixing block 52 is opened, the fixing block 52 is filled with water, the ventilating net is closed, cotton 51 on a parasitic egg placing frame 5 in the first feeding chamber 2 absorbs water, then fresh and full striped rice borer egg blocks are placed on the parasitic egg placing frame 5, the temperature in the first feeding chamber 2 is adjusted to be 26-28 ℃, the air humidity is 75-85%, a test tube filled with black abdominal wasps is placed on a test tube rack 6 in the first feeding chamber 2, and the black abdominal wasps are parasitized after coming out; after parasitizing, opening a baffle plate, communicating a first feeding chamber 2 with a second feeding chamber 3, sliding a parasitized egg placing frame 5 together with parasitized striped borer eggs to transfer to the second feeding chamber 3, sliding the baffle plate to separate the first feeding chamber 2 from the second feeding chamber 3, adjusting the temperature and the humidity of the second feeding chamber 3 to appropriate conditions, taking out the parasitized eggs after a few days before eclosion of the black abdominal ovum bees, placing the parasitized eggs in large test tubes with honey coated on tube walls, and placing the large test tubes on a test tube rack 6 of the first feeding chamber 2 until the bees eclosion; mating the eclosion equi-abdominal black bee in the first feeding room 2, introducing the female bee of the eclosion equi-abdominal black bee into a fingering tube for split charging, and plugging the tube opening with cotton 51; transferring the finger-shaped tube filled with the black oophagous wasps to a low-temperature incubator for storage.
In the above steps, the parasitizing of the black striped rice borer eggs takes about one day, the parasitizing takes about twenty days after the parasitizing and the emergence of the black striped rice borer eggs takes 6-8 hours after the emergence, the prior device, such as patent CN207897735U previously applied by the applicant, is suitable for the parasitizing of the black striped rice borer eggs, but the time required for each stage is different, the device only realizes the propagation of a single stage, the efficiency is low, the device can be simultaneously and continuously carried out, the stages are not influenced by each other, for example, in the first feeding room 2, the parasitized striped rice borer eggs can be continuously inoculated and propagated after being transferred to the second feeding room 3, and the emergence mating stage does not influence the preservation of adult eggs in the second feeding room 3. Thus. The transfer device has the advantages that the practicability is strong, the efficiency is high, meanwhile, the space is saved, compared with the prior art that different devices are used in each breeding stage, the transfer of the parasite eggs of the striped rice borers is more convenient and safer, the damage or water loss and shriveling of the parasite eggs of the striped rice borers are effectively reduced, and the parasitic rate and the emergence rate of inoculation breeding can be improved.
Example 2
A method for indoor preservation of the black bee eggs in the abdomen by using the breeding device in the embodiment 1 comprises the following steps:
(1) inoculation and propagation: after absorbing water in the cotton 51 on the parasitic egg placing rack 5 in the first feeding room 2, placing fresh and plump striped rice borer egg blocks on the parasitic egg placing rack 5, adjusting the temperature in the first feeding room to be 28 ℃, adjusting the air humidity to be 85%, placing test tubes filled with the black-spotted wasps on the test tube rack 6 in the first feeding room 2 according to the ratio of the number of the black-spotted wasps in equal abdomen to the number of eggs of the striped rice borer to be 1:18, and carrying out parasitization for 30 hours;
(2) egg, larva and pupal stage preservation: transferring the parasitic striped rice borer egg blocks together with the parasitic egg placing rack 5 into a second feeding room 3; adjusting the temperature of the second feeding chamber 3 to 17 ℃ and the humidity to 80% by a temperature and humidity controller 9, keeping the cotton 51 on the parasitic egg placing rack 5 moist, and taking out the parasitic egg 2 days before emergence of the black bee;
(3) and (4) adult-period preservation: taking out parasitic ova, placing the parasitic ova in a large test tube with the wall coated with honey, and placing the large test tube on a test tube rack 6 of the first feeding room 2 until the eggs emerge; after the black-spotted wasps in the equal abdomen are fully mated for 8 hours after eclosion, introducing the female wasps in the equal abdomen into a fingering tube for split charging, and plugging the tube opening with cotton 51; transferring the finger-shaped tube filled with the black bee to a low-temperature incubator, and storing at 10 deg.C and 75% air humidity.
Example 3
A method for indoor preservation of the black bee eggs in the abdomen by using the breeding device in the embodiment 1 comprises the following steps:
(1) inoculation and propagation: after absorbing water in the cotton 51 on the parasitic egg placing rack 5 in the first feeding room 2, placing fresh and plump striped rice borer egg blocks on the parasitic egg placing rack 5, adjusting the temperature in the first feeding room to be 26 ℃, adjusting the air humidity to be 75%, placing test tubes filled with the black-spotted wasps on the test tube rack 6 in the first feeding room 2 according to the ratio of the number of the black-spotted wasps in equal abdomen to the number of eggs of the striped rice borer to be 1:15, and carrying out parasitization for 20 hours;
(2) egg, larva and pupal stage preservation: transferring the parasitic striped rice borer egg blocks together with the parasitic egg placing rack 5 into a second feeding room 3; adjusting the temperature of the second feeding chamber 3 to 20 ℃ and the humidity to 80% by a temperature and humidity controller 9, keeping the cotton 51 on the parasitic egg placing rack 5 moist, and taking out the parasitic egg from the parasitic egg placing rack 1 day before the emergence of the black bee;
(3) and (4) adult-period preservation: taking out parasitic ova, placing the parasitic ova in a large test tube with the wall coated with honey, and placing the large test tube on a test tube rack 6 of the first feeding room 2 until the eggs emerge; after the black-spotted wasps in the abdomen are fully mated for 6 hours after eclosion, introducing the female wasps in the black-spotted wasps in the abdomen into a fingering tube for split charging, and plugging the tube opening with cotton 51; transferring the finger-shaped tube filled with the black bee to a low-temperature incubator, and storing at 10 deg.C and 75% air humidity.
Example 4
A method for indoor preservation of the black bee eggs in the abdomen by using the breeding device in the embodiment 1 comprises the following steps:
(1) inoculation and propagation: after absorbing water in the cotton 51 on the parasitic egg placing rack 5 in the first feeding room 2, placing fresh and plump striped rice borer egg blocks on the parasitic egg placing rack 5, adjusting the temperature in the first feeding room to be 27 ℃, adjusting the air humidity to be 80%, placing test tubes filled with the black-spotted wasps on the test tube rack 6 in the first feeding room 2 according to the ratio of the number of the black-spotted wasps in equal abdomen to the number of eggs of the striped rice borer to be 1:20, and carrying out parasitization for 25 hours;
(2) egg, larva and pupal stage preservation: transferring the parasitic striped rice borer egg blocks together with the parasitic egg placing rack 5 into a second feeding room 3; after the temperature and humidity of the second feeding chamber 3 are adjusted to 18 ℃ and 80% by the temperature and humidity controller 9, keeping the cotton 51 on the parasitic egg placing rack 5 wet until 2 days before emergence of the black-egg bees in the abdomen;
(3) and (4) adult-period preservation: taking out parasitic ova, placing the parasitic ova in a large test tube with the wall coated with honey, and placing the large test tube on a test tube rack 6 of the first feeding room 2 until the eggs emerge; after the black-spotted wasps in the abdomen are fully mated for 7 hours after eclosion, introducing the female wasps in the black-spotted wasps in the abdomen into a fingering tube for split charging, and plugging the tube opening with cotton 51; transferring the finger-shaped tube filled with the black bee to a low-temperature incubator, and storing at 10 deg.C and 75% air humidity.
Experiment:
1. the applicant stores the black bee bees in the same abdomen obtained in the step (3) at different temperatures, and the survival time is as follows:
survival conditions of black wasp imagoes in abdomen such as different temperature conditions
The data above demonstrate that adult black-egg bees are stored at 10 degrees, and the survival time of black-egg bees is maximized.
2. The applicant studies the development of the anoectochilus formosanus at different temperatures, and the development results are as follows
Development duration and development rate of black oophagous wasps in equal abdomen at different temperatures
The applicant researches in many aspects, the temperature and the humidity of the environment in the inoculation period are controlled to be 26-28 ℃, the air humidity is 75-85%, the parasitic rate and the emergence rate are highest, the temperature and the humidity for storing eggs, larvae and pupae are controlled to be 17-20 ℃, the humidity is 80%, the black bee broods in the abdomen can normally complete development, the development period is prolonged, the emergence rate is not affected, the black bee broods in the abdomen are stored under the conditions that the temperature is 10 ℃ and the air humidity is 75%, the average life of the female bees is more than one month, and the longest life can reach 82 days. On the basis, the device of the application is used for inoculation, propagation and preservation, and the parasitic rate and the emergence rate of the inoculation and propagation are both improved. Through experiments, compared with the method for breeding and breeding the black-spotted wasps indoors by using different breeding devices in different stages in the same time period in the method of the embodiment 1 to the embodiment 3, the parasitic rate of inoculation and breeding (parasitic rate of eggs of striped rice borer/number of eggs of striped rice borer) of the method can reach more than 90%, the parasitic rate of inoculation and breeding is improved by about 15%, and the emergence rate (bee emergence rate/number of eggs of striped rice borer) can reach more than 90%, and the emergence rate is improved by about 25%.
The above description is intended to describe in detail the preferred embodiments of the present invention, but the embodiments are not intended to limit the scope of the claims of the present invention, and all equivalent changes and modifications made within the technical spirit of the present invention should fall within the scope of the claims of the present invention.
Claims (10)
1. Device is raised to black egg bee such as sugarcane striped rice borer natural enemy, its characterized in that includes:
the device comprises a device body, wherein a first feeding chamber and a second feeding chamber are arranged in the device body; the first feeding chamber and the second feeding chamber are separated by a movable baffle plate;
a plurality of groups of parasitic egg placing frames are respectively arranged in the first feeding chamber and the second feeding chamber; cotton is arranged on the parasitic ovum placing rack;
test tube racks are respectively arranged on two side walls, adjacent to the second feeding chamber, in the first feeding chamber;
an opening with a door leaf is arranged on the side wall of one side of the first feeding chamber opposite to the second feeding chamber; the tops of the first feeding chamber and the second feeding chamber are provided with openable cover bodies; the first feeding chamber and the second feeding chamber are respectively provided with a temperature and humidity controller.
2. The raising device for the black ovalbumin abdominal such as the natural enemy of the sugarcane striped rice borer according to claim 1, wherein the test tube rack is vertically arranged in the raising chamber, and a plurality of accommodating cavities for accommodating test tubes are arranged on the test tube rack.
3. The raising device for the black ovalbumin ventricoverpa such as the natural enemy of the sugarcane striped rice borer according to claim 1, wherein a plurality of groups of slide rails are further arranged at the bottom of the device body from the first raising chamber to the second raising chamber; the parasitic egg placing racks are arranged on the sliding rails in a sliding mode, and openings are formed in the positions, corresponding to the sliding rails, of the baffle plates, so that the end portions of the baffle plates can be completely abutted against the bottom of the device body.
4. The raising device of the black oophagous variegate such as the natural enemy of the sugarcane striped rice borer according to claim 1, wherein the parasitic placing rack comprises a fixed block and two clamping pieces; the fixed block is slidably arranged on the slide rail, and the two clamping pieces are fixed on the fixing block and used for clamping the blade containing the parasitic ovum; the cotton is arranged on the opposite inner sides of the two clamping pieces.
5. The raising device of the black ovalbumin such as the natural enemy of the sugarcane striped rice borer according to claim 4, characterized in that a water storage chamber is arranged in the fixed block; the fixed block is provided with a water injection hole and a water diversion hole, and the water injection hole and the water diversion hole are respectively communicated with the water storage chamber; the water diversion hole is internally provided with water absorption cotton, one end of the water absorption cotton extends and is soaked in the water storage chamber, and the other end of the water absorption cotton is connected with the cotton.
6. The raising device for the black ovalbumin ventricoverum, such as the natural enemy of the sugarcane striped rice borer, according to claim 1, is characterized in that the device body is a transparent box body.
7. The device for raising black ovalbumin such as a natural enemy of sugarcane striped rice borer according to claim 1, wherein a ventilation net is arranged on the water injection hole in an open-close manner.
8. A method for indoor conservation of black bee queen using the rearing device of any one of claims 1 to 7, comprising the steps of:
(1) inoculation and propagation: after cotton on a parasitic egg placing frame in a first feeding room absorbs water, placing fresh and plump striped rice borer egg blocks on the parasitic egg placing frame, adjusting the temperature in the first feeding room to be 26-28 ℃, adjusting the air humidity to be 75-85%, placing a test tube filled with anoplophora ventricosa on a test tube rack in the first feeding room, and carrying out parasitic operation for 20-30 hours;
(2) egg, larva and pupal stage preservation: transferring the parasitic striped rice borer egg blocks and the parasitic egg placing rack to a second feeding room; adjusting the temperature of the second feeding room to be 17-20 ℃ through a temperature and humidity controller, keeping the cotton on the parasitic egg placing rack moist after the humidity is 80%, and taking out the cotton after the cotton is parasitic until 1-2 days before the black oophagous wasps in the abdomen emerge;
(3) and (4) adult-period preservation: taking out parasitic ova, placing the parasitic ova in a large test tube with the wall coated with honey, and placing the large test tube on a test tube rack of a first breeding room until the parasitic ova emerge; after the black oophagous trichogrammae emerges and fully mates for 6-8 hours, introducing the female trichogramma of the black oophagous trichogrammae into a fingering tube for split charging, and plugging the tube opening with cotton; transferring the finger-shaped tube filled with the black oophagous wasps to a low-temperature incubator for storage.
9. The indoor breeding method for the black-egg bees on the equal abdomen, according to claim 8, is characterized in that the ratio of the number of the female bees of the black-egg bees to the number of the eggs of the striped rice borer in the first breeding room is 1: 15-20.
10. The method for indoor breeding of black bee eggs on equal abdomen, according to claim 8, wherein in the step (3), the finger-shaped tube filled with black bee eggs on equal abdomen is preserved under the conditions of 10 ℃ and 75% of air humidity.
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