CN104996236A - Method for preventing and treating dendroctonus valens LeConte by using scleroderma guani Xiao et Wus - Google Patents
Method for preventing and treating dendroctonus valens LeConte by using scleroderma guani Xiao et Wus Download PDFInfo
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- 241001300233 Dendroctonus valens Species 0.000 title claims abstract description 53
- 238000000034 method Methods 0.000 title claims abstract description 34
- 241000467911 Sclerodermus guani Species 0.000 title abstract description 7
- 241000382353 Pupa Species 0.000 claims abstract description 44
- 238000009395 breeding Methods 0.000 claims abstract description 8
- 230000001488 breeding effect Effects 0.000 claims abstract description 7
- 239000011521 glass Substances 0.000 claims abstract description 7
- 229920000742 Cotton Polymers 0.000 claims abstract description 6
- 241000256844 Apis mellifera Species 0.000 claims description 58
- 206010039710 Scleroderma Diseases 0.000 claims description 50
- 230000003071 parasitic effect Effects 0.000 claims description 32
- 241001481710 Cerambycidae Species 0.000 claims description 6
- 230000013011 mating Effects 0.000 claims description 4
- 238000007599 discharging Methods 0.000 claims description 3
- 238000007586 pull-out test Methods 0.000 claims description 3
- 230000008961 swelling Effects 0.000 claims description 3
- 241000027431 Anoplophora Species 0.000 abstract 6
- 241000256856 Vespidae Species 0.000 abstract 2
- 241001481304 Vespoidea Species 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 238000005238 degreasing Methods 0.000 abstract 1
- 238000007789 sealing Methods 0.000 abstract 1
- 231100000225 lethality Toxicity 0.000 description 9
- 230000017448 oviposition Effects 0.000 description 8
- 231100000518 lethal Toxicity 0.000 description 6
- 230000001665 lethal effect Effects 0.000 description 6
- 230000004083 survival effect Effects 0.000 description 5
- 238000004088 simulation Methods 0.000 description 4
- 241000238631 Hexapoda Species 0.000 description 3
- 241000545593 Scolytinae Species 0.000 description 3
- 230000035558 fertility Effects 0.000 description 3
- 230000012447 hatching Effects 0.000 description 3
- 230000024241 parasitism Effects 0.000 description 3
- 230000002265 prevention Effects 0.000 description 3
- 241000254173 Coleoptera Species 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 230000013872 defecation Effects 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 230000004907 flux Effects 0.000 description 2
- 239000011347 resin Substances 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 1
- 241000256852 Aculeata Species 0.000 description 1
- 241001124657 Bethylidae Species 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 241000122992 Chrysidoidea Species 0.000 description 1
- 241000223218 Fusarium Species 0.000 description 1
- 241000122794 Holopyga generosa Species 0.000 description 1
- 240000005979 Hordeum vulgare Species 0.000 description 1
- 235000007340 Hordeum vulgare Nutrition 0.000 description 1
- 241000257303 Hymenoptera Species 0.000 description 1
- 241000018650 Pinus massoniana Species 0.000 description 1
- 235000011610 Pinus tabuliformis Nutrition 0.000 description 1
- 241000219000 Populus Species 0.000 description 1
- 239000005030 aluminium foil Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000010835 comparative analysis Methods 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M99/00—Subject matter not provided for in other groups of this subclass
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M2200/00—Kind of animal
- A01M2200/01—Insects
- A01M2200/012—Flying insects
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Pest Control & Pesticides (AREA)
- Wood Science & Technology (AREA)
- Insects & Arthropods (AREA)
- Engineering & Computer Science (AREA)
- Animal Behavior & Ethology (AREA)
- Animal Husbandry (AREA)
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- General Health & Medical Sciences (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a method for preventing and treating dendroctonus valens LeConte by using scleroderma guani Xiao et Wus. The method comprises: selecting larvas or pupas of anoplophora imitators as hosts for the breeding of scleroderma guani Xiao et Wus, raising parasitic wasps inside glass finger-shaped test tubes, sealing the test tube openings of glass finger-shaped test tubes with degreasing cotton, placing the glass finger-shaped test tubes inside manual climate chambers for culturing, and obtaining a large number of female adult wasps; selecting appropriate weather to release the wasps with every3 to 5 plants acting as a placement position, pulling out cotton plugs from the test tubes, placing the test tubes in an inclined manner at the root portions of trees or placing test tube openings on branchlets of the trees. The scleroderma guani Xiao et Wus can climb out of the test tubes voluntarily, and disperse voluntarily in the trees or among the trees in search for the larvas or pupas of the anoplophora imitators. The scleroderma guani Xiao et Wus of the invention are used in the field of preventing and treating alien invasive anoplophora imitators for the first time, and can effectively cause death to or live on the larvas or pupas of the anoplophora imitators so as to prevent and control forest occurrence rate of the anoplophora imitators, thus addressing the problem of tree death caused by hazards to trees, branches or trunks imposed by the anoplophora imitators.
Description
Technical field
The invention belongs to techniques of pest control field, be specifically related to a kind of method utilizing Scleroderma Xiao et Wu to prevent and treat red turpentine beetle.
Background technology
Scleroderma Xiao et Wu, has another name called Scleroderma guani, formal name used at school: Scleroolerma guani Xiao et Wu.Belong to Hymenoptera Hymenoptera, Aculeata Aculeata, cuckoo wasp Superfamily Chrysidoidea, Bethylidae Bethylidae.Scleroderma Xiao et Wu is the distinctive class epizoite honeybee of China, is mainly used at present in the control of the trunk borers such as coleoptera sky bovine, flatheaded borer class.But be rarely used in the bark beetle class pest preventing and treating forest of causing harm.Utilizing Scleroderma Xiao et Wu to prevent and treat the method for red turpentine beetle, is a kind of domestic effective ways utilizing Scleroderma Xiao et Wu to prevent and treat Alien Invasive Species red turpentine beetle first.
The technical scheme relating to Scleroderma Xiao et Wu application in Chinese patent database has No. 201310220763.2 " swollen leg honeybee carries the method for fusarium prolifertum control poplar ten spot flatheaded borer larva ", number 201110102239.6 " induced scleroderma guani breeding method ", number 201310002442.5 " utilize barley worm breed the method for Scleroderma Xiao et Wu for vector ", number 201010505973.2 " a kind of method for killing tree beetles ", No. 201210529421.4 " a kind of methods of preventing and treating pink neck longicorn " and No. 200410007192.5 " the Scleroderma Xiao et Wu parasitism controls of longicorn " etc., but up to now, have no control Scleroderma Xiao et Wu being used for Alien Invasive Species red turpentine beetle, for the process patent utilizing Scleroderma Xiao et Wu to prevent and treat red turpentine beetle.
Summary of the invention
The object of the present invention is to provide a kind of method utilizing Scleroderma Xiao et Wu to prevent and treat Alien Invasive Species red turpentine beetle, with the woodland incidence of prevention and corntrol red turpentine beetle, thus solution red turpentine beetle harm forest trunk or limb cause a difficult problem for tree death.
The invention provides a kind of method utilizing Scleroderma Xiao et Wu to prevent and treat red turpentine beetle, comprise the following steps:
(1) numerous honeybee: the larva of selection plan longicorn beetle or pupa are as the host breeding Scleroderma Xiao et Wu, and parasitic wasp is raised in glass dactylethrae, and the mouth of pipe seals with absorbent cotton, is then placed in climatic cabinate and cultivates, obtain a large amount of female one-tenth honeybee.
(2) discharge: in red turpentine beetle larva or pupa period, select fair weather, wind-force is little, and temperature is at 20-30 DEG C, and the Guan Shi discharging above-mentioned cultivation swells the female one-tenth honeybee of leg.Need the trees situation of being injured (resin flux, eat hollow, weak or dying trees) investigating generation area before putting honeybee, set honeybee method of putting (put honeybee number of times, put honeybee amount) according to the age of tree of zones of different, thickness of stand, bark beetle occurrence degree.Interval is adopted to put the method for honeybee, at interval of 3-5 strain as a set-point.When putting honeybee, pull out test tube tampon, test tube is tiltedly placed on trees ' root or by mouthpiece cover on the sprig of trees, the described Guan Shi female one-tenth honeybee of leg of swelling can climb out of test tube voluntarily, spreads voluntarily, to find red turpentine beetle larva or pupa on forest or between forest.
Further, by selecting at woodland serious by red turpentine beetle harm and having the individual plant that fresh defecation hole is discharged, investigate the insect density (red turpentine beetle larva or pupa) in every strain phloem burrow, in this, as putting honeybee number of times and the foundation of putting honeybee amount, also can be used as the evaluation of follow-up control efficiency.
Further, the condition of culture in described climatic cabinate is 20-30 DEG C, RH 70%, photoperiod 14:10h.
Further, the described Guan Shi female one-tenth honeybee of leg of swelling is the mating of 5 ages in days.
Beneficial effect of the present invention is: the method utilizing Scleroderma Xiao et Wu to prevent and treat red turpentine beetle of the present invention, Scleroderma Xiao et Wu is utilized to prevent and treat the method for Strategy of Alien Invasive Species red turpentine beetle first, can reach more than 45% to the lethality rate of red turpentine beetle mature larva, effective parasitic rate is between 30 ~ 65%; Can reach more than 85% to the lethality rate of red turpentine beetle pupa, effective parasitic rate is between 50 ~ 86%, and the female one-tenth honeybee of parasitic wasp filial generation accounts for 89%; Simulation red turpentine beetle is caused harm phloem, and parasitic rate is between 40 ~ 72%, and parasitic wasp filial generation female one-tenth honeybee ratio is between 65 ~ 90%.
Efficiently solve utilize the effectively lethal or parasitic red turpentine beetle of Scleroderma Xiao et Wu larva, pupa.Wherein, best with the parasitic effects of pupa, to the breeding effectively blocking or suppress red turpentine beetle, can be used as the key factor of field release critical period.With the woodland incidence of prevention and corntrol red turpentine beetle, thus solution red turpentine beetle harm forest trunk or limb cause a difficult problem for tree death.
Accompanying drawing explanation
Figure 1 shows that the present invention utilizes Scleroderma Xiao et Wu to prevent and treat in the embodiment of the method 1 of red turpentine beetle Scleroderma Xiao et Wu to the lethal cases figure of RTB larva and pupa;
Figure 2 shows that the present invention utilizes Scleroderma Xiao et Wu to prevent and treat in the embodiment of the method 1 of red turpentine beetle Scleroderma Xiao et Wu to the sneak case figure of RTB larva and pupa;
Figure 3 shows that the present invention utilize Scleroderma Xiao et Wu prevent and treat red turpentine beetle embodiment of the method 1 in Scleroderma Xiao et Wu on RTB larva and pupa preoviposition period and oviposition duration figure;
Figure 4 shows that the present invention utilizes Scleroderma Xiao et Wu to prevent and treat the reproduction of Scleroderma Xiao et Wu on RTB larva and pupa in the embodiment of the method 1 of red turpentine beetle and tries hard to;
Figure 5 shows that the present invention utilizes Scleroderma Xiao et Wu to prevent and treat the filial generation ratio figure of Scleroderma Xiao et Wu on RTB larva and pupa in the embodiment of the method 1 of red turpentine beetle;
Figure 6 shows that the present invention utilizes Scleroderma Xiao et Wu to prevent and treat the filial generation incubation rate of Scleroderma Xiao et Wu on RTB larva and pupa and survival rate figure in the embodiment of the method 1 of red turpentine beetle;
Figure 7 shows that the present invention utilizes Scleroderma Xiao et Wu to prevent and treat in the embodiment of the method 1 of red turpentine beetle under simulated conditions Scleroderma Xiao et Wu to the parasitic rate figure of RTB.
Embodiment
Hereafter will describe the specific embodiment of the invention in detail in conjunction with concrete accompanying drawing.It should be noted that the combination of technical characteristic or the technical characteristic described in following embodiment should not be considered to isolated, they can mutually be combined thus be reached better technique effect.
Embodiment 1
(1) numerous honeybee: the larva of selective advantage vector plan longicorn beetle or pupa are as the host breeding Scleroderma Xiao et Wu, parasitic wasp is raised at glass dactylethrae (diameter: 1.2cm, length: 7.5cm) in, the mouth of pipe seals with absorbent cotton, then 25 ± 5 DEG C are placed on, RH 70%, cultivate in the climatic cabinate of photoperiod 14:10h, to obtain a large amount of female one-tenth honeybee, this honeybee source meets the following conditions: individuality is large, energy is strong, and be in optimum utilization period (5 ages in days, mating).
(2) honeybee is connect: the three kinds of worm states choosing red turpentine beetle (below all referred to as RTB) are as host, and as low instar larvae, mature larva and pupa, often kind of each 30 repetitions of worm state, carry out connecing honeybee (worm honeybee is than being 1:1) respectively.Observe from connecing after honeybee, observed once every 12 hours, record female one-tenth honeybee to the lethality rate of different host condition and sneak case, comprise following biological parameter: female one-tenth honeybee preoviposition period (my god), oviposition duration (my god), fecundity (single female egg laying amount, grain/♀); The egg hatching rate (%) of filial generation, filial generation adult survival rate (%), filial generation ratio (the male proportion of filial generation, ♂ %).According to the observation, record and data analysis, calculate the successful parasitic rate (%) of female one-tenth honeybee to the different host condition of RTB.
(3) lab simulation: choose the Chinese pine trunk of causing harm by red turpentine beetle, intercept trunk 1.2m with the phloem of lower portion (20cm × 12cm), be placed in self-control insect box (208 × 138 × 128mm), box inner bottom part places the aseptic paper handkerchief of one deck, cover one deck aluminium foil thereon again, to ensure certain humidity, be similar to micro-border of dwelling of RTB field survivorship, be also beneficial to parasitic wasp and effectively can search host.In addition, connect the parasitic rate after honeybee for the ease of statistics, on this bark of manual control, the quantity (larva 50 in 2-3 age, pupa 50) of RTB, chooses unnecessary RTB.Then connect honeybee, 20 repetitions, observe, record effective parasitic rate (%) of female one-tenth honeybee to RTB.
(4) assessment of control efficiency: carry out statistical analysis according to the data in (2) and (3), assess female one-tenth honeybee to the parasitic capacity of red turpentine beetle, thus under assessment indoor conditions, Scleroderma Xiao et Wu is to the control efficiency of red turpentine beetle.The parameter of assessment Parasitic Fitness comprises: Scleroderma Xiao et Wu is to the lethality rate (%) of RTB, effectively parasitic rate (%); Female one-tenth honeybee preoviposition period (my god), oviposition duration (my god), fecundity (single female egg laying amount, grain/♀); The egg hatching rate (%) of filial generation, filial generation adult survival rate (%), filial generation ratio (the male proportion of filial generation, ♂ %).
Scleroderma Xiao et Wu to the lethal of RTB low instar larvae, mature larva and pupa and sneak case, as depicted in figs. 1 and 2.Wherein, the lethality rate of Scleroderma Xiao et Wu to RTB low instar larvae is about 25%, and effective parasitic rate is lower, <2%; But be about 56% to the lethality rate of RTB mature larva, effective parasitic rate can increase to 51%; And the highest to the lethality rate of RTB pupa, about reach about 88%, effective parasitic rate can reach more than 75%.The control of this parasitic wasp for RTB is described, is more suitable for mature larva phase of RTB and release in pupa time, guarantee Scleroderma Xiao et Wu, thus can free diffusing at woodland to effective parasitism of RTB, and the field being convenient to this biological control method is implemented.
Scleroderma Xiao et Wu is to the Parasitic Fitness comparative analysis of RTB mature larva and pupa, and see that in Fig. 3-6, figure, black represents RTB mature larva, white represents RTB pupa.As shown in Figure 3, Guan Shi swell the female one-tenth honeybee of leg on RTB mature larva and pupa preoviposition period be about 10 days, oviposition duration 2-3 days.But Guan Shi swells, and there were significant differences for the fecundity of the female one-tenth honeybee of leg on both, and the female egg laying amount of the list on RTB mature larva is only about 9, and the female egg laying amount of list on RTB pupa can reach about 18, is the former 2 times (as shown in Figure 4).
As shown in Figure 5, on RTB pupa mature larva and pupa, the property of filial generation honeybee is than all with female worm large percentage, significantly partially female, accounts for 89%.Illustrate Scleroderma Xiao et Wu can on RTB pupa mature larva and pupa successful reproduction or parasitism, provide important evidence to field release tech from now on.
The filial generation survival condition of Scleroderma Xiao et Wu on RTB mature larva and pupa, as shown in Figure 6.On RTB mature larva, the egg hatching rate of filial generation honeybee about reaches 66%, and can increase to 89% on RTB pupa.Equally, filial generation becomes the survival rate of honeybee on RTB pupa higher, is 73%, is 2 times on RTB mature larva.
Lab simulation field be injured bark method inoculation, Scleroderma Xiao et Wu to the lethal of RTB and sneak case, as shown in Figure 7.Scleroderma Xiao et Wu can be successfully searched larva and the pupa of RTB under simulated conditions, and can be successful lethal or parasitic.
Embodiment 2
(1) numerous honeybee: the larva of selective advantage vector plan longicorn beetle or pupa are as the host breeding Scleroderma Xiao et Wu, parasitic wasp is raised at glass dactylethrae (diameter: 1.2cm, length: 7.5cm) in, the mouth of pipe seals with absorbent cotton, then 25 ± 5 DEG C are placed on, RH 70%, cultivate in the climatic cabinate of photoperiod 14:10h, to obtain a large amount of female one-tenth honeybee, this honeybee source meets the following conditions: individuality is large, energy is strong, and be in optimum utilization period (5 ages in days, mating).
(2) discharge: in red turpentine beetle larva or pupa period, select fair weather, wind-force is little, and temperature is at 20-30 DEG C, and the Guan Shi discharging above-mentioned cultivation swells the female one-tenth honeybee of leg.Need the trees situation of being injured (resin flux, eat hollow, weak or dying trees) investigating generation area before putting honeybee, set honeybee method of putting (put honeybee number of times, put honeybee amount) according to the age of tree of zones of different, thickness of stand, bark beetle occurrence degree.Interval is adopted to put the method for honeybee, at interval of 3-5 strain as a set-point.When putting honeybee, pull out test tube tampon, test tube is tiltedly placed on trees ' root or by mouthpiece cover on the sprig of trees, Scleroderma Xiao et Wu can climb out of test tube voluntarily, spreads voluntarily on forest or between forest, to find red turpentine beetle larva or pupa.
By selecting at woodland serious by red turpentine beetle harm and having the individual plant that fresh defecation hole is discharged, investigate the insect density (red turpentine beetle larva or pupa) in every strain phloem burrow, in this, as putting honeybee number of times and the foundation of putting honeybee amount, also can be used as the evaluation of follow-up control efficiency.
The present invention utilizes Scleroderma Xiao et Wu to prevent and treat the method for Strategy of Alien Invasive Species red turpentine beetle first, and can reach more than 45% to the lethality rate of red turpentine beetle mature larva, effective parasitic rate is between 30 ~ 65%; Can reach more than 85% to the lethality rate of red turpentine beetle pupa, effective parasitic rate is between 50 ~ 86%, and the female one-tenth honeybee of parasitic wasp filial generation accounts for 89%; Simulation red turpentine beetle is caused harm phloem, and parasitic rate is between 40 ~ 72%, and parasitic wasp filial generation female one-tenth honeybee ratio is between 65 ~ 90%.
Efficiently solve utilize the effectively lethal or parasitic red turpentine beetle of Scleroderma Xiao et Wu larva, pupa.Wherein, best with the parasitic effects of pupa, to the breeding effectively blocking or suppress red turpentine beetle, can be used as the key factor of field release critical period.With the woodland incidence of prevention and corntrol red turpentine beetle, thus solution red turpentine beetle harm forest trunk or limb cause a difficult problem for tree death.
Although give some embodiments of the present invention, it will be understood by those of skill in the art that without departing from the spirit of the invention herein, can change embodiment herein.Above-described embodiment is exemplary, should using embodiment herein as the restriction of interest field of the present invention.
Claims (3)
1. utilize Scleroderma Xiao et Wu to prevent and treat a method for red turpentine beetle, it is characterized in that, comprise the following steps:
(1) numerous honeybee: the larva of selection plan longicorn beetle or pupa are as the host breeding Scleroderma Xiao et Wu, and parasitic wasp is raised in glass dactylethrae, and the mouth of pipe seals with absorbent cotton, is then placed in climatic cabinate and cultivates, obtain female one-tenth honeybee;
(2) discharge: in red turpentine beetle larva or pupa period, select fair weather, wind-force is little, temperature is at 20-30 DEG C, the Guan Shi discharging above-mentioned cultivation swells the female one-tenth honeybee of leg, adopts interval to put honeybee, at interval of 3-5 strain as a set-point, when putting honeybee, pull out test tube tampon, test tube is tiltedly placed on trees ' root or by mouthpiece cover on the sprig of trees, the described Guan Shi female one-tenth honeybee of leg of swelling can climb out of test tube voluntarily, spread voluntarily on forest or between forest, to find red turpentine beetle larva or pupa.
2. utilize Scleroderma Xiao et Wu to prevent and treat the method for red turpentine beetle as claimed in claim 1, it is characterized in that, the condition of culture in described climatic cabinate is 20-30 DEG C, RH 70%, photoperiod 14:10h.
3. utilize Scleroderma Xiao et Wu to prevent and treat the method for red turpentine beetle as claimed in claim 1, it is characterized in that, described female one-tenth honeybee is 5 ages in days, and mating.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106359366A (en) * | 2016-09-19 | 2017-02-01 | 云南农业大学 | Method for preventing and controlling chestnut weevils with scleroderma guani |
| CN106718395A (en) * | 2016-12-01 | 2017-05-31 | 何振贤 | It is a kind of to discharge the method that the swollen leg honeybee of Kazakhstan prevents and treats Chinese scholar tree control of Apriona swainsoni |
| CN106718537A (en) * | 2016-12-01 | 2017-05-31 | 何振贤 | A kind of method for discharging the swollen leg honeybee biological control willow anoplophora glabripennis of Kazakhstan |
| CN106900659A (en) * | 2017-02-27 | 2017-06-30 | 贵州师范大学 | A kind of utilization Scleroderma Xiao et Wu preventing and treating lasioderma serricorne larva and the method for pupa |
| CN109221022A (en) * | 2018-10-29 | 2019-01-18 | 云南省烟草公司昆明市公司 | Aphid parasite product automation process units |
| CN112616778A (en) * | 2020-12-12 | 2021-04-09 | 四川农业大学 | Method for keeping parasitic capacity of artificially fed Szechwan scleroderma and application of method |
| CN113079904A (en) * | 2021-03-29 | 2021-07-09 | 中资蓝天生态环境科技有限公司 | Method for preventing Dendrolimus punctatus beetles in forestry and landscape architecture |
| CN116250510A (en) * | 2023-04-26 | 2023-06-13 | 伊犁哈萨克自治州林业科学研究院 | Artificial rearing method for leg-swelling bees |
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| CN116250510B (en) * | 2023-04-26 | 2024-06-04 | 伊犁哈萨克自治州林业科学研究院 | Artificial rearing method for leg-swelling bees |
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