CN1663368A

CN1663368A - Scleroderma guani parasitism control for loghorn beetle

Info

Publication number: CN1663368A
Application number: CN 200410007192
Authority: CN
Inventors: 王素英; 时亚琴; 陆群
Original assignee: INNER MONGOLIA AUTONOMOUS REGION RESEARCH INSTITUTE OF FORESTRY SCIENCES
Current assignee: INNER MONGOLIA AUTONOMOUS REGION RESEARCH INSTITUTE OF FORESTRY SCIENCES
Priority date: 2004-03-03
Filing date: 2004-03-03
Publication date: 2005-09-07

Abstract

The prevention and cure using parasitization of scleroderma guani Xiao et Wu for pest of Capricorn beetle is a method that introduces a kind of scleroderma guani Xiao et Wu of parasitization and cultivation to biocide capricorn beetle grubs, which comprises two parts, parasitic breeding of scleroderma guani Xiao et Wu and the biocide of adults to capricorn beetle grubs. The parasitic method is to oviposite of bethylidae in the grubs and pupae of Saperda populnea Linnaeus, Asias halodendri Pallas and into grubs' body of three ages of Anoplophora glabripenis (Motschulsky), Anoplophora nobilis Ganglbauer and red neck capricorn beetle, and gain the adult scleroderma guani Xiao et Wu after the growth during ovum stage, larval stage and pupal stage. Then, placing the adults in forest at opportune moment, where they can find grubs in tree or spread carrying bacterial on road or body of insects, which makes capricorn beetle be killed when young. The prevention and cure effect can be more than 95%, which is active to ecological construction and forest production.

Description

The parasitic control of the swollen leg honeybee of the Guan Shi of longicorn

Technical field

The present invention relates to the plant insect control, particularly relate to the longicorn control of insect of trees, is the parasitic Prevention Technique of the swollen leg honeybee of a kind of Guan Shi.

Background technology

As everyone knows, longicorn is fatal to the injury of trees, scope also is widely, China, Korea S, America and Europe, generation is all arranged, especially mid-term of the seventies to the beginning of the eighties, the poplar in area, North China, willow and elm were reached more than 80% by the harm face of longicorn once, the northwest-north-northeast China networks of shelterbelts are ruined, brought heavy losses to production of forestry.The kind of longicorn is a lot of, trees all there is harm, the anoplophora glabripennis main harm be broad-leaved tree, red-edge longicorn main harm sea-buckthorn, pink neck longicorn main harm north fruit tree, Monochamus alternatus Hope main harm pine tree, can cause that the pine wilt nematode disease is damaged pine forest, become the cancer of forest, because of these seeds major parts all are distributed in the north, therefore, the existence of longicorn is to the north, particularly the potential destruction of the fragile ecological environment of central and west regions is huge, and being apparent that has influenced township afforestation and shelterbelt construction.After the eighties, existing in the world many countries are studied the control of longicorn, the U.S. starts from 1997 to the research of anoplophora glabripennis, mainly be at geographical, vegetation and weather to its behavior in aspect the diffusion influence, and it is also few to the research of pathogen and insect parasite, really, do not see have what achievement to come out.

Each provinces and regions, China North China, comprise Chinese forest-science academy, Beijing Lin Da etc., since the project verification eighties anoplophora glabripennis is studied, initial research is based on biological property, the pest-resistant seeds of seed selection, forestry measure and chemical control, and it is fewer to its biological control, even carried out the investigation and the collection of natural enemy kind, do not reach application yet, can be used as the natural enemy of anoplophora glabripennis though for example find the hard first of flower suede, but to only not breeding at present utilization, to the later stage nineties, just begin to pay attention to the research of biological control aspect.Starting Inner Mongol forestry scientific research institute early when the sea-buckthorn trunk borer comprehensive regulation research of setting about carrying out, finds that swollen leg honeybee can produce effect to the control of red-edge longicorn.Purpose of the present invention deeply changes this achievement exactly, specialize and applicationizations, and in the same way, and the leg honeybee of the swelling parasitic Prevention Technique of carrying disease germs is promoted the use of during all longicorns prevent and treat, and will play positive protective effect to ecological construction and agricultural production undoubtedly.

Summary of the invention

The parasitic control of the swollen leg honeybee of Guan Shi of the present invention longicorn technology comprises following two aspects:

(1) parasitized breeding of the swollen leg honeybee (Soleroderma gucmi xiao ce wu) of Guan Shi---allow swollen leg honeybee in particular environment, lay eggs in larva and the pupa of Cathay poplar longicorn (Saperdapopulne), red-edge longicorn (Margites Gahan), China fir longicorn (Semanotus), and in the anoplophora glabripennis (Anoplophoraglobripennis), yellow spotted star longicorn beetle (Anoplophora nobilis), red neck longicorn (Aromia buhgll) 3 instar larvae bodies (Fald), grow through larval phase, pupa time and adult stage, parasitized breeding becomes the Guan Shi leg honeybee of swelling.

(2) the release control behind the swollen leg bee imago---the swollen leg honeybee of Guan Shi that will grow adult discharges in the trees woods, makes it to seek the roundheaded borer host and kills and propagate the bacterial classification that carries and kill.Described bacterial classification is that a kind of is powder Paecilomyces varioti fungi Z from the anoplophora glabripennis isolated two kinds of disease funguses in the body of falling ill ₂₆, a kind of is beauveria bassiana Z ₂₈, these two kinds of bacterial classifications all have toxic action to all longicorns.

Embodiment

The parasitic control of the swollen leg honeybee of Guan Shi of the present invention longicorn technology is a kind of application technology of BIOLOGICAL CONTROL longicorn harm, and range of application comprises anoplophora glabripennis, red-edge longicorn, red neck longicorn, pine and cypress longicorn, China fir longicorn, also can be used for the control to other trunk borer.

The concrete parasitized breeding way of the swollen leg honeybee of Guan Shi of the present invention: trees, the branch that will have above-mentioned larva are adopted back from the field, bleaching powder seminal fluid with 0.5% soaks does 5～10 minutes the sterilization in surface, under aseptic condition, in the trees body, take out larva, pack in the dactylethrae of bore 1.2cm, long 12cm, one of every pipe, insert two of swollen leg honeybees then, put into culture plate, be 75% in humidity, temperature is to raise in 20～22 ℃ the gnotobasis, then swollen leg honeybee can lay eggs in the larva body, more swollen leg honeybee is migrated in another raising pipe after having produced ovum.Afterwards, when treating the interior swollen leg honeybee egg development of roundheaded borer body to larval phase humidity being dropped to 40～50% scopes raises, connecting leg moves together and is put into 4 ℃ of environment and preserves standby when growing to pupa time, when to be needed, the more swollen leg honeybee of the Guan Shi in pupa time is taken out to move in the environment that is placed on 25 ℃ from low temperature environment and be cultured to adult;

Adult is discharged the way of preventing and treating of woodland: direct method (generally at young larva)---with cultured adult, deliver to and pour tree fork in the woods into and get final product, selection of time is at 5～June, calm and weather that temperature is high.Put into the swollen leg honeybee of the Guan Shi that sticks of tree and can find the longicorn host very soon, can parasitic larva be body length at 1cm with interior young larva, general had 30～40 days can breed a generation under the condition of 15～25 ℃ of daily mean temperatures, and therefore the young larva of longicorn is killed; Indirect method (generally being at aged larva)---allow the swollen leg honeybee of adult carry disease germs, its way is, will be from the anoplophora glabripennis isolated powder paecilomycerol Z in the body that falls ill ₂₆With beauveria bassiana Z ₂₈Make conidial powder, both mix, and put make its band powder when discharging swollen leg honeybee after again, and the swollen leg honeybee of the powder that then carries disease germs falls and the bacterium powder can be infected growth and breeding in the gallery behind the tree, and make larva infected and then kill longicorn, perhaps make cingula parasitism to the larva body make it dead.These two kinds of powder bacterium can temperature difference complementation, can both be in gallery growth and breeding, above-mentioned way can make total control efficiency reach more than 95%.

The concrete pre-stage test of implementing shows: two kinds of disease funguses can reach 85%～100% to the parasitic rate of anoplophora glabripennis, if utilize synergist to produce fungi, its sporulation quantity comparison is according to increasing by 16 times, this parasitic lethality to anoplophora glabripennis can be compared according to increasing by 11.1%, and all parasitic speed can be done sth. in advance about 15 days.Thus, the control cost of every strain tree is about 2 yuan

Technology path of the present invention: be on the achievement basis of pre-stage test, carry out technological improvement, assembling and further conversion, enlarge range of application, reduce application cost, carry out the pilot experiment in land for growing field crops.

Social benefit of the present invention from the control to anoplophora glabripennis, is transformed in the control of all longicorn, and the forest that can make China various places avoids the harm of longicorn and growing way is strong tall and straight, and the ecological construction in two areas obtains good progress in making.

Claims

1, the parasitic control of the swollen leg honeybee of the Guan Shi of longicorn, its feature includes two parts:

A, the parasitized breeding of the swollen leg honeybee of Guan Shi---allow swollen leg honeybee in particular environment, lay eggs in larva and the pupa of Cathay poplar longicorn, red-edge longicorn, China fir longicorn and to lay eggs and make in the larva body in 3 of anoplophora glabripennis, yellow spotted star longicorn beetle, red neck longicorn, grow through ovum phase, larval phase, pupa time and adult stage, parasitized breeding becomes the Guan Shi leg honeybee of swelling.

B, the release control of the swollen leg bee imago that breeding is good---the swollen leg honeybee of Guan Shi that will grow adult discharges in the trees woods, makes it to seek the insecticidal microorganism bacterium powder that roundheaded borer host in the tree body or propagation are carried.

2, by the parasitic control of the swollen leg honeybee of the Guan Shi of claim item 1 described longicorn, its concrete parasitized breeding way is: the trees that will have above-mentioned larva, branch is adopted back from the field, bleaching powder seminal fluid with 0.5% soaks did surface disinfection in 5～10 minutes, under aseptic condition, in the trees body, take out larva, bore 1.2cm packs into, in the dactylethrae of long 12cm, one of every pipe, insert two of swollen leg honeybees then, put into culture plate, in humidity is 75%, temperature is to raise in 20～22 ℃ the gnotobasis, and then swollen leg honeybee can lay eggs in the larva body, more swollen leg honeybee is migrated in another raising pipe after having produced ovum.Afterwards, treat that when swollen leg honeybee egg development on the roundheaded borer body is to larval phase humidity being dropped to 40～50% scopes raises, connecting leg moves together and is put into 4 ℃ of environment and preserves standby during to teleiophan stage, when to be needed, the more swollen leg honeybee of the Guan Shi in pupa time is taken out to move in the environment that is placed on 25 ℃ from low temperature environment and be cultured to adult.

3, by the parasitic control of the swollen leg honeybee of the Guan Shi of claim item 1 and 2 described longicorns, the selection of time that wherein adult is discharged woodland is at 5～June, calm and weather that temperature is high.

4, by the parasitic control of the swollen leg honeybee of the Guan Shi of claim item 1 and 2 described longicorns, feature is that the swollen leg honeybee of adult carries disease germs that to plant be from the anoplophora glabripennis isolated powder paecilomycerol Z in the body that falls ill ₂₆With beauveria bassiana Z ₂₈, make to mix after the conidial powder and use, when discharging swollen leg honeybee, make its powder that carries disease germs.