CN103355261B - The perpendicular hair roundheaded borer of fiber crops is as the application of host in Scleroderma Xiao et Wu artificial propagation - Google Patents
The perpendicular hair roundheaded borer of fiber crops is as the application of host in Scleroderma Xiao et Wu artificial propagation Download PDFInfo
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- CN103355261B CN103355261B CN201310329392.1A CN201310329392A CN103355261B CN 103355261 B CN103355261 B CN 103355261B CN 201310329392 A CN201310329392 A CN 201310329392A CN 103355261 B CN103355261 B CN 103355261B
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Abstract
The present invention relates to insect and raise field, especially relate to Scleroderma Xiao et Wu artificial propagation techniques.The invention provides the perpendicular hair roundheaded borer of fiber crops as the application of host in Scleroderma Xiao et Wu artificial propagation, the perpendicular hair longicorn artificial culture technology of fiber crops is comparatively ripe, and cultivation amount is large, can realize large-scale breeding.It can be used as the Hosts of Scleroderma Xiao et Wu, parasitism is strong, and Scleroderma Xiao et Wu survival rate is high.
Description
Technical field
The present invention relates to insect and raise field, especially relate to Scleroderma Xiao et Wu artificial propagation techniques.
Background technology
Scleroderma Xiao et Wu is as Semanotus bifasciatus sinoauster, and Saperda Populnea, the larva of the multiple borer pest such as Osier weevil and the epizoite honeybee of pupa are the Natural Enemies of above-mentioned borer pest.Along with pest damage area and the continuous expansion of area, the technology of preventing and treating borer pest with Scleroderma Xiao et Wu also comes into one's own gradually.But because Scleroderma Xiao et Wu just must can complete growth by live body host, and the collection of the large-scale longicorn such as anoplophora glabripennis and artificial feeding more difficult, therefore find Hosts, realizing large-scale breeding becomes important topic.
Summary of the invention
First technical problem solved by the invention is to provide the perpendicular hair roundheaded borer of fiber crops as the application of host in Scleroderma Xiao et Wu artificial propagation.The perpendicular hair longicorn artificial culture technology of fiber crops is comparatively ripe, and cultivation amount is large, can realize large-scale breeding.It can be used as the Hosts of Scleroderma Xiao et Wu, parasitism is strong, and Scleroderma Xiao et Wu survival rate is high.
It is artificial feeding that fiber crops of the present invention erect mao roundheaded borer.
The present invention further provides a kind of method of Scleroderma Xiao et Wu artificial propagation, it is with the host of the perpendicular hair roundheaded borer of fiber crops as Scleroderma Xiao et Wu parasitism.
Concrete embodiment can be: erect in the dactylethrae of hair roundheaded borer by the access of Scleroderma Xiao et Wu kind honeybee containing fiber crops with writing brush, jam-pack tampon; The dactylethrae inoculated is put into culturing room cultivate, the temperature in culturing room controls at 24 DEG C ~ 28 DEG C, and optimum is 26 DEG C; Every day is detected, and takes out mouldy host larva in time, adds fresh host larva; Regularly carry out disinfection in incubation, take a breath, respectively take a breath in morning every day, noon, evening 10min ~ 20min.
Through about 30d in culturing room, Scleroderma Xiao et Wu starts to sprout wings, and is taken out by the Scleroderma Xiao et Wu after emergence in time, put 2d ~ 3d at room temperature, then select from culturing room.
In culturing room, to the egg hatching phase preoviposition period of Scleroderma Xiao et Wu, relative moisture controls 75% ~ 80%; Larva to pupa time, relative moisture 60% ~ 65%; Emergence period relative moisture is more than 75%.
When honeybee is planted in access, the perpendicular hair roundheaded borer of fiber crops is preferably 1:2 with the worm honeybee ratio of kind of honeybee, numb perpendicular hair longicorn 4 in every dactylethrae.
As breeding swollen leg honeybee out wouldn't use, 2 DEG C ~ 5 DEG C refrigerators or freezer Cord blood can be put in rear pupa time or first emergence period.Temperature is down to suitable temperature gradually by gradient from high to low, and the holding time should more than 90d.
The present invention is under fiber crops erect mao prerequisite of longicorn artificial breeding technology, and by utilizing fiber crops perpendicular hair roundheaded borer access Scleroderma Xiao et Wu as high-quality host, the scale achieving Scleroderma Xiao et Wu is expanded numerous.
Embodiment
For further illustrating the present invention, illustrate with the following Examples:
Embodiment 1:
One, instrument and equipment:
1. laboratory is equipped with incubator, box for feeding pests, anatomical lens, microscope, superclean bench.
2. culturing room is equipped with wooden culturing rack, ultraviolet germicidal lamp, humidifier, air conditioner, brethaid, temperature and humidity control instrument, climatic cabinate, insulating box, biochemical cultivation case, temp. and humidity from record instrument etc.
3. transfer room is equipped with operating desk, sterilizing equipment decontaminating apparatus and inoculating appliance.
4. refrigerating chamber is equipped with refrigerator, refrigerator, refrigeration plant.
Two, main apparatus and running stores: white porcelain dish, glass dactylethrae, culture dish, writing brush, tweezers, scalpel, pruner, raising basket, tampon, Polypropylence Sheet, rubber band, disinfectant.
Three, numerous honeybee host
1. host prepares
1.1 numerous honeybee hosts are that healthy fiber crops erect hair roundheaded borer.
The collections of the perpendicular hair roundheaded borers of 1.2 fiber crops are in annual August, rive and get worm, make being erected a mao piemarker root for longicorn harm by fiber crops to be separated between individuality as far as possible, avoid cutting one another's throat during collection.Also the fiber crops sending out scale artificial propagation can be used to erect the larva of mao longicorn.
2. host requires: numerous honeybee host larva should be flexible, not susceptible healthy live body.
3. host's storage: had by the parasitism adopted back the chingma abutilon root of the perpendicular hair longicorn of fiber crops to put into woven bag, be deposited in 2 DEG C ~ 5 DEG C refrigerating chambers and preserve, also directly can store larva.Period of storage should more than 180d.
Four, plant honeybee to prepare
1. kind of honeybee obtains
1.1 field acquisitions gather swollen leg bee imago in the wild or cut open larva or the cocoon that swollen leg honeybee collected by wood, take back indoor, and larva or cocoon are placed in culturing room or constant incubator to continue to be cultured to grows for adult, and temperature controls at 25 DEG C ~ 28 DEG C.The swollen leg bee imago adopted back in field and the adult the turned out perpendicular hair roundheaded borer of fiber crops carry out breeding conservation.
1.2 introduce a fine variety or introduce from other Fan Feng units.
2. kind of the method that honeybee selection detects is: randomly draw the kind honeybee pipe of 8% ~ 10%, and microscopy statistics has the quantity of wing queen bee and apterergate.When there being the quantity of wing queen bee to exceed 30% of queen bee sum, this population is unfit to do honeybee kind.Select healthy and strong, glossy, energetic apterergate to do kind of a honeybee, eliminate weak honeybee.
3., when numerous honeybee is not carried out in the storage of kind of honeybee, plant in the refrigerator or freezer that honeybee is stored in 2 DEG C ~ 5 DEG C.Preservation process checks the survival condition of kind of honeybee at any time.Summer holding time should more than 180d, and winter should more than 120d.
Five, breeding with honeybee is prevented and treated
1. the kind honeybee of storage is taken out by kind of honeybee process, and carry out warm honeybee, temperature controls at about 25 DEG C.
2. articles for use prepare that dactylethrae, cotton plug are some, ceramic whiteware pallet, culture dish, writing brush, scalpel, tweezers, plastic crate, sterilization apparatus etc.
3., before sterilization connects honeybee, culturing room's application ultraviolet etc. carries out disinfection, and all apparatus adopt high temperature or additive method sterilization, sterilization 24h, ventilation 6h.Non-sterile, can not carry out inoculating and cultivating.
4. the worm honeybee ratio that worm honeybee perpendicular hair longicorn number than application breeds is generally 1:2.
5. connect the perpendicular hair longicorn often pipe 4 of honeybee fiber crops.With writing brush by the honeybee kind dactylethrae of access according to the above ratio containing the perpendicular hair roundheaded borer of fiber crops respectively, jam-pack tampon.Honeybee kind at least should derive from more than 3 different kind honeybee pipes.Connect every day after honeybee kind terminates, use alcohol scrubbing utensil.
6. cultivate and the dactylethrae inoculated is put into culturing room cultivate.Every day is detected, and takes out mouldy host larva in time.Regularly carry out disinfection in incubation, take a breath.Respectively take a breath in morning every day, noon, evening 10min ~ 20min.
7. the temperature in Temperature and Humidity Control culturing room controls at 24 DEG C ~ 28 DEG C, optimum temperature 26 DEG C.Preoviposition period to the egg hatching phase, relative moisture controls 75% ~ 80%; Larva to pupa time, relative moisture 60% ~ 65%; Emergence period relative moisture is more than 75%.
8. go out honeybee through about 30d in culturing room, Scleroderma Xiao et Wu starts to sprout wings, and is taken out by the Scleroderma Xiao et Wu after emergence in time, put 2d ~ 3d at room temperature, then select from culturing room.
9. storage such as breeding swollen leg honeybee out wouldn't use, and can put Cord blood in people's 2 DEG C ~ 5 DEG C refrigerators or freezer in rear pupa time or first emergence period.Temperature is down to suitable temperature gradually by gradient from high to low, and the holding time should more than 90d.
According to the method for above-described embodiment, erect hair longicorn as host using fiber crops, the perpendicular hair longicorn of every only fiber crops goes out honeybee about 40.
Comparative example: adopt anoplophora glabripennis host as an alternative, Scleroderma Xiao et Wu cannot benumb host, its larva cannot be parasitic.
Above-described embodiment is only be described the preferred embodiment of the present invention; not scope of the present invention is limited; under not departing from the present invention and designing the prerequisite of spirit; the various distortion that the common engineers and technicians in this area make technical scheme of the present invention and improvement, all should fall in protection domain that claims of the present invention determine.
Claims (4)
1. a method for Scleroderma Xiao et Wu artificial propagation, is characterized in that: erect in the dactylethrae of hair roundheaded borer by the access of Scleroderma Xiao et Wu kind honeybee containing fiber crops with writing brush, jam-pack tampon; The dactylethrae inoculated is put into culturing room cultivate, the temperature in culturing room controls at 28 DEG C; Every day is detected, and takes out mouldy host larva in time, adds fresh host larva; Regularly carry out disinfection in incubation, take a breath, respectively take a breath in morning every day, noon, evening 10min ~ 20min.
2. method according to claim 1, is characterized in that: through about 30d in culturing room, and Scleroderma Xiao et Wu starts to sprout wings, and is taken out by the Scleroderma Xiao et Wu after emergence in time, put 2d ~ 3d at room temperature, then select from culturing room.
3. method according to claim 1, is characterized in that: in culturing room, and to the egg hatching phase preoviposition period of Scleroderma Xiao et Wu, relative moisture controls 75% ~ 80%; Larva to pupa time, relative moisture 60% ~ 65%; Emergence period relative moisture is more than 75%.
4. the method according to any one of claim 1-3, is characterized in that: when honeybee is planted in access, and the perpendicular hair roundheaded borer of fiber crops is 1:2 with the worm honeybee ratio of kind of honeybee, numb perpendicular hair longicorn 4 in every dactylethrae.
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| CN201310329392.1A CN103355261B (en) | 2013-07-30 | 2013-07-30 | The perpendicular hair roundheaded borer of fiber crops is as the application of host in Scleroderma Xiao et Wu artificial propagation |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105028338A (en) * | 2015-07-14 | 2015-11-11 | 贵州师范大学 | Method utilizing larvae and pupae of anoplophora imitators for breeding scleroderma guani |
| CN106376545B (en) * | 2016-09-19 | 2019-09-17 | 云南农业大学 | A kind of method of efficient preservation Scleroderma Xiao et Wu |
| CN106359313A (en) * | 2016-09-28 | 2017-02-01 | 天津拓华生物科技有限责任公司 | Method for breeding bethylids by using Callosobruchus chinensis |
| CN107114326B (en) * | 2017-05-17 | 2020-07-10 | 中国林业科学研究院森林生态环境与保护研究所 | Artificial breeding method of Monochamus alternatus Hope and application of method in prevention and treatment of trunk-boring pests in forest trees |
| CN107821339A (en) * | 2017-12-12 | 2018-03-23 | 中国林业科学研究院森林生态环境与保护研究所 | A kind of artificial breeding method of Monochamus alternatus Hope zanjon cocoon honeybee and its application |
| CN109874750B (en) * | 2019-03-22 | 2021-09-03 | 遵义市林业科学研究所 | Artificial breeding method of Cereus |
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| CN1465239A (en) * | 2002-07-31 | 2004-01-07 | 北京市植物园 | Scaled breeding for semanotus bifasciatus and application in biological preventing and controlling pest-insect |
| CN1663368A (en) * | 2004-03-03 | 2005-09-07 | 内蒙古自治区林业科学研究院 | Scleroderma guani parasitism control for loghorn beetle |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1465239A (en) * | 2002-07-31 | 2004-01-07 | 北京市植物园 | Scaled breeding for semanotus bifasciatus and application in biological preventing and controlling pest-insect |
| CN1663368A (en) * | 2004-03-03 | 2005-09-07 | 内蒙古自治区林业科学研究院 | Scleroderma guani parasitism control for loghorn beetle |
Non-Patent Citations (1)
| Title |
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| 《管氏肿腿蜂人工繁殖及在防治栗山天牛中的应用》;娄杰等;《辽宁林业科技》;20051231(第6期);29-30 * |
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