CN105028338A - Method utilizing larvae and pupae of anoplophora imitators for breeding scleroderma guani - Google Patents
Method utilizing larvae and pupae of anoplophora imitators for breeding scleroderma guani Download PDFInfo
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- CN105028338A CN105028338A CN201510409344.2A CN201510409344A CN105028338A CN 105028338 A CN105028338 A CN 105028338A CN 201510409344 A CN201510409344 A CN 201510409344A CN 105028338 A CN105028338 A CN 105028338A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Abstract
The invention belongs to the technical field of insect feeding and particularly relates to a method utilizing larvae and pupae of anoplophora imitators for breeding scleroderma guani. The method comprises the step of using the larvae and the pupae of the anoplophora imitators as substitute hosts to breed generations of the scleroderma guani. The method comprises the following specific steps of collecting and conserving the larvae and the pupae of the anoplophora imitators, breeding the generations of the scleroderma guani, and screening the optimal bee sources. According to the method, the new host source provided by the method is used for artificially breeding the scleroderma guani, the parasitic capacity and the fertility of the scleroderma guani are improved, and the offspring femina proportion of the scleroderma guani is increased, and therefore the problems that due to the fact that the parasitic capacity of the scleroderma guani artificially bred is reduced along with long-term breeding, offspring individuals are small and the male proportion is increased are solved.
Description
Technical field
The invention belongs to technical field of insect feeding, be specifically related to a kind of method utilizing the larva of plan longicorn beetle and pupa to breed Scleroderma Xiao et Wu.
Background technology
Scleroderma Xiao et Wu, has another name called Scleroderma guani, formal name used at school: ScleroolermaguaniXiaoetWu.Belong to Hymenoptera Hymenoptera, Aculeata Aculeata, cuckoo wasp Superfamily Chrysidoidea, Bethylidae Bethylidae.Scleroderma Xiao et Wu is the distinctive class epizoite honeybee of China, is mainly used at present in the control of the trunk borers such as coleoptera sky bovine, flatheaded borer class.The insect that in production, the swollen leg honeybee of application carried out control has: Saperda Populnea, Monochamus alternatus Hope, semanotus bifasciatus, Semanotus bifasciatus sinoauster, anoplophora glabripennis, mountain longicorn, batocera horsfieldi, longicorn beetle etc.Effective to small-sized longicorn, to large-scale longicorn effect control as grasped the suitable honeybee phase that puts and putting honeybee amount, also can achieve good results.Utilize natural enemy prevent and treat trunk borer have with worm control worm, biotic population internal balance, free from environmental pollution, can for a long time, the advantage of lasting, stability contorting disaster, release enemy's rear area, sky, natural enemy understands Automatic-searching also attack trunk borer, and control efficiency is fine.
In order to better for the prevention and control of the forest tree borers such as longicorn, large quantity research has been done for the method for Scleroderma Xiao et Wu artificial breeding in prior art, existing artificial fecundation method is mainly bred by finding host, large scale artificial breeding host's majority is Saperda Populnea, Monochamus alternatus Hope, the natural hosts such as semanotus bifasciatus, such as application number be 201310329392.1 patent of invention disclose the perpendicular hair roundheaded borer of fiber crops as the application of host in Scleroderma Xiao et Wu artificial propagation, provide the perpendicular hair roundheaded borer of fiber crops as the application of host in Scleroderma Xiao et Wu artificial propagation, the perpendicular hair longicorn artificial culture technology of fiber crops is comparatively ripe, cultivation amount is large, large-scale breeding can be realized.It can be used as the Hosts of Scleroderma Xiao et Wu, parasitism is strong, and Scleroderma Xiao et Wu survival rate is high.Application number be 201310002442.5 patent of invention disclose and a kind ofly utilize barley worm to breed the method for Scleroderma Xiao et Wu for vector, comprise kind of honeybee to obtain, store, increase the steps such as numerous, rejuvenation, expansion are numerous, quality inspection, packaging, storage transport, main use barley worm as an alternative host carry out increasing numerous Scleroderma Xiao et Wu.But existingly undertaken by host that Scleroderma Xiao et Wu artificial fecundation method still exists parasitic capacity, fertility is low, low, the individual less shortcoming of filial generation female ratio, as can be seen here, a kind of new host can be provided to originate, for the artificial breeding of Scleroderma Xiao et Wu, to improve the parasitic capacity of Scleroderma Xiao et Wu, fertility and filial generation female ratio, thus the parasitic capacity of the Scleroderma Xiao et Wu of solution artificial breeding is with breeding for a long time, and parasitic capacity declines, offspring individual is less and the problem of male increasing proportion, becomes the technical barrier that those skilled in the art are urgently to be resolved hurrily.
Summary of the invention
The present invention, in order to solve the problems of the technologies described above, provided a kind of method utilizing the larva of plan longicorn beetle and pupa to breed Scleroderma Xiao et Wu.
Technical scheme of the present invention comprises:
Utilize and intend the method that the larva of longicorn beetle and pupa breed Scleroderma Xiao et Wu, comprising the larva that uses and intend longicorn beetle and the pupa continuous generation that host carries out Scleroderma Xiao et Wu as an alternative breeds.
Specifically comprise the following steps:
Step one: collect and preserve the larva and pupa of intending longicorn beetle: choose and physically well develop and the plan longicorn beetle larva of N/D and pupa confession examination, its body surface is sterilized, after distilled water, 10% alcohol washes, then is cleaned with distilled water, after its body surface dries naturally, weigh;
Step 2: the continuous generation of Scleroderma Xiao et Wu breeds: Scleroderma Xiao et Wu kind honeybee is colonized in and intends in the larva of longicorn beetle and pupa, and by raising in glass dactylethrae, the mouth of pipe seals with absorbent cotton, is then placed in climatic cabinate and cultivates, to obtain a large amount of female one-tenth honeybee;
Step 3: screen best honeybee source: assess the energy of described female one-tenth honeybee obtained, parasitic capacity, screens the optimum condition of cultivating honeybee kind: 5-6 age in days after sprouting wings, and mating.
Further, described glass dactylethrae is of a size of 12 × 75mm, described glass dactylethrae and climatic cabinate all carry out high-temperature sterilization before connecing honeybee and raising, in cultivating in climatic cabinate, daily check 1-2 time, observe and record the sneak case of parasitic wasp, when finding that there is host longicorn by infected by microbes such as white muscardine fungis, taking out in time, clearing up by the host longicorn of infected by microbes.
Further, the condition of culture in described climatic cabinate is: temperature 25 ± 5 DEG C, RH70%, and the photoperiod, namely the time compared for 14h: 10h round the clock.
Further, described Hosts often heavy 0.1g inoculate 1 Scleroderma Xiao et Wu.
Beneficial effect of the present invention comprises: the present invention utilizes first and intends the larva of longicorn beetle, pupa as breeding host, and Scleroderma Xiao et Wu is bred in indoor, can reach more than 85% to the effective parasitic rate intending longicorn beetle; Intend, on the larva of longicorn beetle, being about 4 days preoviposition period of parasitic wasp, the female amount of single female product reaches about 28, and filial generation survival rate is up to 72.4%.In addition, on the larva of intending longicorn beetle and pupa, parasitic wasp filial generation female one-tenth honeybee ratio is respectively 78.8%, 85.6%; Filial generation female one-tenth honeybee body weight all can reach 0.008mg.Comprehensive analysis, intend on the larva of longicorn beetle, pupa, Scleroderma Xiao et Wu has the female amount of high yield, filial generation honeybee high viability, female ratio higher, and these features are all conducive to the biological control of this parasitic wasp.The vector kind efficiently solving artificial breeding Scleroderma Xiao et Wu is few, fertility is not high, the difficult problem such as parasitic capacity degeneration in reproductive process.
Accompanying drawing explanation
Figure 1 shows that the present invention utilizes Scleroderma Xiao et Wu Scleroderma Xiao et Wu intending the sneak case figure on longicorn beetle larva and pupa;
Figure 2 shows that the present invention utilizes Scleroderma Xiao et Wu Scleroderma Xiao et Wu intending the preoviposition period situation map on longicorn beetle larva and pupa;
Figure 3 shows that the present invention utilizes Scleroderma Xiao et Wu Scleroderma Xiao et Wu intending the fecundity situation map on longicorn beetle larva and pupa;
Figure 4 shows that the present invention utilizes Scleroderma Xiao et Wu Scleroderma Xiao et Wu at the filial generation incubation rate intended on longicorn beetle larva and pupa and survival rate situation map;
Figure 5 shows that the present invention utilizes the filial generation of Scleroderma Xiao et Wu Scleroderma Xiao et Wu on plan longicorn beetle larva and pupa to compare situation map.
Embodiment
Hereafter will describe the specific embodiment of the invention in detail in conjunction with concrete accompanying drawing.It should be noted that the combination of technical characteristic or the technical characteristic described in following embodiment should not be considered to isolated, they can mutually be combined thus be reached better technique effect.
Utilize the larva of plan longicorn beetle and pupa to breed a method for Scleroderma Xiao et Wu, specifically comprise the following steps:
Step one: collect and preserve the larva and pupa of intending longicorn beetle: choose and physically well develop and the plan longicorn beetle larva of N/D and pupa confession examination, its body surface is sterilized, after distilled water, 10% alcohol washes, then is cleaned with distilled water, after its body surface dries naturally, weigh;
Step 2: the continuous generation of Scleroderma Xiao et Wu breeds: use and intend the larva of longicorn beetle and the pupa continuous generation that host carries out Scleroderma Xiao et Wu as an alternative and breed, Scleroderma Xiao et Wu kind honeybee is colonized in and intends in the larva of longicorn beetle and pupa, Hosts often heavy 0.1g inoculates 1 Scleroderma Xiao et Wu, and raise in glass dactylethrae, described glass dactylethrae is of a size of 12 × 75mm, described glass dactylethrae and climatic cabinate all carry out high-temperature sterilization before connecing honeybee and raising, the mouth of pipe seals with absorbent cotton, and mark, then be placed in climatic cabinate and cultivate, to obtain a large amount of female one-tenth honeybee.In cultivating in climatic cabinate, daily check 1-2 time, observe and record the sneak case of parasitic wasp, when finding that there is host longicorn by infected by microbes such as white muscardine fungis, timely taking-up, clear up by the host longicorn of infected by microbes, carry out observing, recording and comparative analysis, observing time till its filial generation is sprouted wings completely, observes once every 12h from the female one-tenth honeybee of access simultaneously.Condition of culture in climatic cabinate is: temperature 25 ± 5 DEG C, RH70%, photoperiod 14: 10h;
Step 3: screen best honeybee source: assess the energy of described female one-tenth honeybee obtained, parasitic capacity, screens the optimum condition of cultivating honeybee kind: 5-6 age in days after sprouting wings, and mating;
Step 4: the testing and appraisal of fertility: record Scleroderma Xiao et Wu is intending the sneak case on the larva of longicorn beetle, pupa, result as shown in Figure 1, the comparative analysis of line correlation index of going forward side by side.By female one-tenth honeybee preoviposition period, single female egg laying amount and the female rate of product, be illustrated in figure 2 Scleroderma Xiao et Wu Scleroderma Xiao et Wu and intend the preoviposition period situation map on longicorn beetle larva and pupa, be illustrated in figure 3 Scleroderma Xiao et Wu Scleroderma Xiao et Wu and intend the fecundity situation map on longicorn beetle larva and pupa, and the incubation rate of filial generation honeybee, survival rate, body weight and property are than isoparametric network analysis, thus the efficiency of longicorn beetle as middle Hosts is intended in comprehensive assessment, be illustrated in figure 4 Scleroderma Xiao et Wu Scleroderma Xiao et Wu at the filial generation incubation rate intended on longicorn beetle larva and pupa and survival rate situation map, be illustrated in figure 5 and utilize the filial generation of Scleroderma Xiao et Wu Scleroderma Xiao et Wu on plan longicorn beetle larva and pupa to compare situation map.Through Fig. 1 ~ 5, data result is known, the generation of longicorn beetle is intended in the parasitic wasp honeybee source prevention and control that application the inventive method obtains, be conducive to promoting maintaining ecological balance, keep bio-diversity, the problems such as contaminated solution environment, this invention is the local insect occurrence characteristic of collection, obtains sufficient host insect source, arrives the multi-faceted advanced technology of breeding plan longicorn beetle being integrated as one such as raising parasitic wasp breeding power and parasitic capacity etc. again.
The present invention utilizes first and intends the larva of longicorn beetle, pupa as breeding host, and Scleroderma Xiao et Wu is bred in indoor, can reach more than 80%, can reach more than 85% to effective parasitic rate of pupa to the effective parasitic rate intending longicorn beetle larva; Intend, on the larva of longicorn beetle, being about 4 days preoviposition period of parasitic wasp, the female amount of single female product reaches 27.216, and filial generation survival rate is up to 72.4%.In addition, on the larva of intending longicorn beetle and pupa, parasitic wasp filial generation female one-tenth honeybee ratio is respectively 78.8%, 85.6%; Filial generation female one-tenth honeybee body weight all can reach 0.008mg.Comprehensive analysis, intend on the larva of longicorn beetle, pupa, Scleroderma Xiao et Wu has the female amount of high yield, filial generation honeybee high viability, female ratio higher, and these features are all conducive to the biological control of this parasitic wasp.The vector kind efficiently solving artificial breeding Scleroderma Xiao et Wu is few, fertility is not high, the difficult problem such as parasitic capacity degeneration in reproductive process.
Above-mentioned detailed description is the illustrating of possible embodiments for invention, and this embodiment is also not used to limit the scope of the claims of the present invention, does not allly depart from equivalence of the present invention and implements or change, and all should be contained in the scope of the claims of the present invention.
In addition, those skilled in the art also can make various amendments in other form and details, interpolation and replacement in the claims in the present invention scope of disclosure and spirit.Certainly, the changes such as these various amendments made according to the present invention's spirit, interpolation and replacement, all should be included within the present invention's scope required for protection.
Claims (5)
1. utilize and intend the method that the larva of longicorn beetle and pupa breed Scleroderma Xiao et Wu, it is characterized in that, comprising the larva that uses and intend longicorn beetle and the pupa continuous generation that host carries out Scleroderma Xiao et Wu as an alternative breeds.
2. a kind of method utilizing the larva of plan longicorn beetle and pupa to breed Scleroderma Xiao et Wu according to claim 1, is characterized in that, specifically comprise the following steps:
Step one: collect and preserve the larva and pupa of intending longicorn beetle: choose and physically well develop and the plan longicorn beetle larva of N/D and pupa confession examination, its body surface is sterilized, after distilled water, 10% alcohol washes, then is cleaned with distilled water, after its body surface dries naturally, weigh;
Step 2: the continuous generation of Scleroderma Xiao et Wu breeds: Scleroderma Xiao et Wu kind honeybee is colonized in and intends in the larva of longicorn beetle and pupa, and by raising in glass dactylethrae, the mouth of pipe seals with absorbent cotton, is then placed in climatic cabinate and cultivates, to obtain a large amount of female one-tenth honeybee;
Step 3: screen best honeybee source: assess the energy of described female one-tenth honeybee obtained, parasitic capacity, screens the optimum condition of cultivating honeybee kind: 5-6 age in days after sprouting wings, and mating.
3. a kind of method utilizing the larva of plan longicorn beetle and pupa to breed Scleroderma Xiao et Wu according to claim 2, it is characterized in that, described glass dactylethrae is of a size of 12 × 75mm, described glass dactylethrae and climatic cabinate all carry out high-temperature sterilization before connecing honeybee and raising, in cultivating in climatic cabinate, daily check 1-2 time, observe and record the sneak case of parasitic wasp, when finding that there is host longicorn by infected by microbes such as white muscardine fungis, taking out in time, clearing up by the host longicorn of infected by microbes.
4. a kind of method utilizing the larva of plan longicorn beetle and pupa to breed Scleroderma Xiao et Wu according to claim 2, it is characterized in that, the condition of culture in described climatic cabinate is: temperature 25 ± 5 DEG C, RH70%, and the photoperiod, namely the time compared for 14h: 10h round the clock.
5. a kind of method utilizing the larva of plan longicorn beetle and pupa to breed Scleroderma Xiao et Wu according to claim 2, it is characterized in that, described Hosts often heavy 0.1g inoculates 1 Scleroderma Xiao et Wu.
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Cited By (6)
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| CN105918257A (en) * | 2016-04-14 | 2016-09-07 | 伊犁哈萨克自治州林业科学研究院 | Method for artificially breeding Sclerodermus pupariae by using local alternate hosts |
| CN106359313A (en) * | 2016-09-28 | 2017-02-01 | 天津拓华生物科技有限责任公司 | Method for breeding bethylids by using Callosobruchus chinensis |
| CN107114326A (en) * | 2017-05-17 | 2017-09-01 | 中国林业科学研究院森林生态环境与保护研究所 | A kind of artificial breeding method of the swollen leg honeybee of Monochamus alternatus Hope and the application in preventing and treating forest trunk borer |
| CN108935337A (en) * | 2018-06-26 | 2018-12-07 | 江苏省林业科学研究院 | A technique for promoting Scleroderma Xiao et Wu breeding success rate and reproductive number |
| CN114391515A (en) * | 2022-01-19 | 2022-04-26 | 贵州师范大学 | Evaluation method for indoor control effect of Sciadopitys yunnanensis by using scleroderma guani and application of evaluation method |
| CN117136913A (en) * | 2023-08-31 | 2023-12-01 | 浙江大学 | Indoor propagation method for grassland caterpillar wasp |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105918257A (en) * | 2016-04-14 | 2016-09-07 | 伊犁哈萨克自治州林业科学研究院 | Method for artificially breeding Sclerodermus pupariae by using local alternate hosts |
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| CN107114326A (en) * | 2017-05-17 | 2017-09-01 | 中国林业科学研究院森林生态环境与保护研究所 | A kind of artificial breeding method of the swollen leg honeybee of Monochamus alternatus Hope and the application in preventing and treating forest trunk borer |
| CN107114326B (en) * | 2017-05-17 | 2020-07-10 | 中国林业科学研究院森林生态环境与保护研究所 | Artificial breeding method of Monochamus alternatus Hope and application of method in prevention and treatment of trunk-boring pests in forest trees |
| CN108935337A (en) * | 2018-06-26 | 2018-12-07 | 江苏省林业科学研究院 | A technique for promoting Scleroderma Xiao et Wu breeding success rate and reproductive number |
| CN108935337B (en) * | 2018-06-26 | 2021-04-02 | 江苏省林业科学研究院 | Technology for raising breeding success rate and breeding number of scleroderma guani |
| CN114391515A (en) * | 2022-01-19 | 2022-04-26 | 贵州师范大学 | Evaluation method for indoor control effect of Sciadopitys yunnanensis by using scleroderma guani and application of evaluation method |
| CN114391515B (en) * | 2022-01-19 | 2023-01-31 | 贵州师范大学 | Evaluation method for indoor control effect of Sciadopitys yunnanensis by using scleroderma guani and application of evaluation method |
| CN117136913A (en) * | 2023-08-31 | 2023-12-01 | 浙江大学 | Indoor propagation method for grassland caterpillar wasp |
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