CN108935337A - A technique for promoting Scleroderma Xiao et Wu breeding success rate and reproductive number - Google Patents

A technique for promoting Scleroderma Xiao et Wu breeding success rate and reproductive number Download PDF

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Publication number
CN108935337A
CN108935337A CN201810667542.2A CN201810667542A CN108935337A CN 108935337 A CN108935337 A CN 108935337A CN 201810667542 A CN201810667542 A CN 201810667542A CN 108935337 A CN108935337 A CN 108935337A
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China
Prior art keywords
longicorn
success rate
alcohol
body surface
promoting
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CN201810667542.2A
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CN108935337B (en
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徐丽丽
郑华英
解春霞
刘云鹏
高悦
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Nanjing Kanglinbao Biological Technology Co Ltd
Jiangsu Forestry Academy
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Nanjing Kanglinbao Biological Technology Co Ltd
Jiangsu Forestry Academy
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Housing For Livestock And Birds (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The present invention relates to the technologies of a kind of breeding success rate for promoting Scleroderma Xiao et Wu and reproductive number, the present invention has screened the infected rate that suitable body surface disinfection reagent, processing method and suitable disinfecting time effectively reduce longicorn, promotes breeding success rate and reproductive number.Compared to control group, 24.88% is promoted by the longicorn breeding success rate that this technology is handled, reproductive number is more than that 50 ratios promote 13.56%, effectively improves the success rate and reproductive number of swollen leg bee breeding.

Description

A technique for promoting Scleroderma Xiao et Wu breeding success rate and reproductive number
Technical field
The present invention relates to a kind of breeding methods of insect epizoite bee, and in particular to a kind of to promote the numerous of Scleroderma Xiao et Wu Grow the technology of success rate and reproductive number.
Background technique
Scleroderma Xiao et Wu is under the jurisdiction of Hymenoptera Bethylidae, is the epizoite natural enemy of a variety of longicorn class borer pests Insect.Longicorn class insect is important forest borer pest, mainly eats into food trunk, branch or tree root with larva and causes harm, is Harmful seriousness, has brought tremendous economic losses to society, seriously affected China afforestation project, Desertification Control and Construction of city afforestation.Since the roundheaded borer phase is longer and is in inside host, difficulty of prevention and cure is relatively large, is often difficult to reach ideal The application of effect, natural enemy is increasingly taken seriously.Wherein, Scleroderma Xiao et Wu is since it can distributed areas be wide, adaptability is high, search energy Power is strong, host types are more, is widely used during Harmful prevention and treatment.But in the artificial breeding mistake of Scleroderma Xiao et Wu Cheng Zhong, find host's biting of self or it is infected cause polypide to black, the phenomena of mortality it is serious, it is dead after host can not be by swollen leg Bee is parasitic, and breeding success rate is lower, considerably increases breeding cost.
Summary of the invention
The present invention is that host longicorn is infected serious in effective solution reproductive process, the low existing issue of breeding success rate, The technology effectively reduces being felt for longicorn by screening suitable body surface disinfection reagent, processing method and suitable disinfecting time Dye rate promotes breeding success rate and reproductive number.
To achieve the above object, present invention provide the technical scheme that
A method of promoting Scleroderma Xiao et Wu breeding success rate and reproductive number, comprising the following steps:
(1) selection of longicorn: the longicorn of healthy uninfection is chosen;
(2) body surface cleans: carrying out easy clean to longicorn body surface, wipes moisture, the sawdust, excretion of the attachment of longicorn body surface Object;
(3) alcohol disinfecting degerming: longicorn is poured into alcohol, is gently mixed, and is fully immersed in longicorn in alcohol, uses wine Essence disinfection degerming;
(4) it dries for the first time: soaked longicorn is sieved, remove alcohol, be placed in the draught cupboard that ultraviolet disinfection sterilized It is interior, until longicorn body surface alcohol takes out after volatilizing completely;
(5) hydrogen peroxide secondary sterilization degerming: the longicorn dried is poured into hydrogen peroxide, is gently mixed, soaks longicorn completely Bubble is in hydrogen peroxide, with hydrogen peroxide secondary sterilization degerming;
(6) dry for the second time: by treated, longicorn is placed in the draught cupboard that ultraviolet disinfection sterilized, until longicorn body surface Hydrogen peroxide takes out after volatilizing completely and carries out normal Scleroderma Xiao et Wu inoculation.
In step (1), selection is normally flexible, tail portion final section is not in spasticity and body surface has no wound or blackspot Longicorn is as host to be selected.
In step (2), longicorn is placed in clean plastic screen screen frame, toilet paper is kneaded into loose spherical shape, day is placed in It is gently wiped above ox body surface, longicorn is driven to roll, wipe moisture, the sawdust, excreta of the attachment of longicorn body surface.
In step (3), biggish container is chosen, the 75% concentration alcohol of 3 times of longicorn volumes is poured into, longicorn is poured into alcohol In, it is gently mixed using blunt nosed tweezers, guarantees that all longicorns are fully immersed in alcohol.Soaking time is 5 minutes, soaking process Slight jitter container is needed, prevents longicorn from biting mutually.
In step (5), the longicorn dried is poured into the hydrogen peroxide of 3% volumetric concentration, wherein hydrogen peroxide is 3 times of longicorn bodies Product, is gently mixed using blunt nosed tweezers, guarantees that all longicorns are fully immersed in hydrogen peroxide.Soaking time is 3 minutes, is impregnated Journey needs slight jitter container, prevents longicorn from biting mutually.
Compared with prior art, the beneficial effects of the present invention are:
The present invention has screened suitable body surface disinfection reagent, processing method and suitable disinfecting time and has effectively reduced longicorn Infected rate promotes breeding success rate and reproductive number.Compared to control group, the longicorn breeding success rate handled by this technology Promote 24.88%, reproductive number is more than that 50 ratios promote 13.56%, effectively improve the success rate of swollen leg bee breeding with Reproductive number.
Specific embodiment
The present invention is further explained in the light of specific embodiments.
A method of promoting Scleroderma Xiao et Wu breeding success rate and reproductive number, comprising the following steps:
(1) it the selection of longicorn: chooses and normally stretches, tail portion final section is not in spasticity and body surface has no wound or black The longicorn of spot is as host to be selected.
(2) body surface cleans: longicorn being placed in clean plastic screen screen frame, toilet paper is kneaded into loose spherical shape, is placed in It is gently wiped above longicorn body surface, longicorn is driven to roll, wipe moisture, sawdust, the excreta etc. of the attachment of longicorn body surface.
(3) alcohol disinfecting degerming: choosing biggish container, pours into the 75% concentration alcohol of 3 times of longicorn volumes, longicorn is fallen Enter in alcohol, be gently mixed using blunt nosed tweezers, guarantee that all longicorns are fully immersed in alcohol, soaking time is 5 minutes, leaching Bubble process needs slight jitter container, prevents longicorn from biting mutually.
(4) dry: by soaked longicorn, sieving removes alcohol, is placed in the draught cupboard that ultraviolet disinfection sterilized, directly It is taken out after volatilizing completely to longicorn body surface alcohol.
(5) hydrogen peroxide secondary sterilization degerming: the longicorn dried is poured into the hydrogen peroxide of 3% volumetric concentration, wherein dioxygen Water is 3 times of longicorn volumes, is gently mixed using blunt nosed tweezers, guarantees that all longicorns are fully immersed in hydrogen peroxide, soaking time It is 3 minutes, soaking process needs slight jitter container, prevents longicorn from biting mutually.
(6) dry: by treated, longicorn is placed in the draught cupboard that ultraviolet disinfection sterilized, until longicorn body surface hydrogen peroxide It is taken out after volatilization completely and carries out normal Scleroderma Xiao et Wu inoculation.
It is tested by above step, is directly inoculated with and is compared with the longicorn being not handled by, set 9 repetitions altogether, often One repeats interior test longicorn 100.As a result, it has been found that untreated longicorn breeding success rate is 29.78% ± 6.080%, wherein Every pipe reproductive number is more than 50 account for inoculated tube number 11.56% ± 4.137%, is 54.67% ± 6.349% after processing, It is 25.11% ± 4.341% that wherein every pipe reproductive number, which is more than 50,.Compared to control group, the day handled by this technology Ox breeding success rate promotes 24.88%, and reproductive number is more than that 50 ratios promote 13.56%, and it is numerous to effectively improve swollen leg bee The success rate and reproductive number grown.
The above is only presently preferred embodiments of the present invention, is not intended to limit the present invention in any form, any ripe Professional and technical personnel is known, without departing from the scope of the present invention, according to the technical essence of the invention, to the above reality Any simple modifications, equivalent substitutions and improvements etc. made by example are applied, it is fallen within the scope of protection of the technical scheme of the present invention It is interior.

Claims (7)

1. a kind of method for promoting Scleroderma Xiao et Wu breeding success rate and reproductive number, it is characterised in that: the following steps are included:
(1) selection of longicorn: the longicorn of healthy uninfection is chosen;
(2) body surface cleans: carrying out easy clean to longicorn body surface, wipes moisture, the sawdust, excreta of the attachment of longicorn body surface;
(3) alcohol disinfecting degerming: longicorn is poured into alcohol, is gently mixed, and is fully immersed in longicorn in alcohol, is disappeared with alcohol Malicious degerming;
(4) it dries for the first time: soaked longicorn is sieved, remove alcohol, be placed in the draught cupboard that ultraviolet disinfection sterilized, directly It is taken out after volatilizing completely to longicorn body surface alcohol;
(5) hydrogen peroxide secondary sterilization degerming: the longicorn dried is poured into hydrogen peroxide, is gently mixed, longicorn is fully immersed in In hydrogen peroxide, with hydrogen peroxide secondary sterilization degerming;
(6) dry for the second time: by treated, longicorn is placed in the draught cupboard that ultraviolet disinfection sterilized, until longicorn body surface dioxygen Water takes out after volatilizing completely and carries out normal Scleroderma Xiao et Wu inoculation.
2. the method according to claim 1 for promoting Scleroderma Xiao et Wu breeding success rate and reproductive number, it is characterised in that: In step (1), choosing normal flexible, tail portion final section is not in the longicorn conduct that spasticity and body surface have no wound or blackspot Host to be selected.
3. the method according to claim 1 for promoting Scleroderma Xiao et Wu breeding success rate and reproductive number, it is characterised in that: In step (2), longicorn is placed in clean plastic screen screen frame, toilet paper is kneaded into loose spherical shape, is placed on longicorn body surface Side gently wipes, and longicorn is driven to roll, and wipes moisture, the sawdust, excreta of the attachment of longicorn body surface.
4. the method according to claim 1 for promoting Scleroderma Xiao et Wu breeding success rate and reproductive number, it is characterised in that: In step (3), biggish container is chosen, the 75% concentration alcohol of 3 times of longicorn volumes is poured into, longicorn is poured into alcohol, is used Blunt nosed tweezers are gently mixed, and guarantee that all longicorns are fully immersed in alcohol.
5. the method according to claim 4 for promoting Scleroderma Xiao et Wu breeding success rate and reproductive number, it is characterised in that: Soaking time is 5 minutes, and soaking process needs slight jitter container, prevents longicorn from biting mutually.
6. the method according to claim 1 for promoting Scleroderma Xiao et Wu breeding success rate and reproductive number, it is characterised in that: In step (5), the longicorn dried is poured into the hydrogen peroxide of 3% volumetric concentration, wherein hydrogen peroxide is 3 times of longicorn volumes, is used Blunt nosed tweezers are gently mixed, and guarantee that all longicorns are fully immersed in hydrogen peroxide.
7. the method according to claim 6 for promoting Scleroderma Xiao et Wu breeding success rate and reproductive number, it is characterised in that: Soaking time is 3 minutes, and soaking process needs slight jitter container, prevents longicorn from biting mutually.
CN201810667542.2A 2018-06-26 2018-06-26 Technology for raising breeding success rate and breeding number of scleroderma guani Active CN108935337B (en)

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US5352593A (en) * 1992-09-18 1994-10-04 American Apiary Farm Company Production of fuel alcohol from honey
CN102599116A (en) * 2012-04-09 2012-07-25 天津师范大学 Method for artificial feeding of Zeuzera leuconolum Butler
CN103168753A (en) * 2013-04-17 2013-06-26 香格里拉县宜康宝生物科技有限公司 Method for culturing hepialus larvae indoors
CN104322451A (en) * 2014-08-20 2015-02-04 广东省昆虫研究所 Manual low-altitude raising method for host insect hepialidae of ophiocordyceps sinensis
KR101519550B1 (en) * 2013-11-21 2015-05-15 김종훈 Fishbowl
CN104642267A (en) * 2015-02-09 2015-05-27 云南绿叶生防科技有限公司 Industrial cultivation method for aphidius gifuensis ashmead
CN105028338A (en) * 2015-07-14 2015-11-11 贵州师范大学 Method utilizing larvae and pupae of anoplophora imitators for breeding scleroderma guani

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5352593A (en) * 1992-09-18 1994-10-04 American Apiary Farm Company Production of fuel alcohol from honey
CN102599116A (en) * 2012-04-09 2012-07-25 天津师范大学 Method for artificial feeding of Zeuzera leuconolum Butler
CN103168753A (en) * 2013-04-17 2013-06-26 香格里拉县宜康宝生物科技有限公司 Method for culturing hepialus larvae indoors
KR101519550B1 (en) * 2013-11-21 2015-05-15 김종훈 Fishbowl
CN104322451A (en) * 2014-08-20 2015-02-04 广东省昆虫研究所 Manual low-altitude raising method for host insect hepialidae of ophiocordyceps sinensis
CN104642267A (en) * 2015-02-09 2015-05-27 云南绿叶生防科技有限公司 Industrial cultivation method for aphidius gifuensis ashmead
CN105028338A (en) * 2015-07-14 2015-11-11 贵州师范大学 Method utilizing larvae and pupae of anoplophora imitators for breeding scleroderma guani

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