CN110934130A - Method for manufacturing ornamental fish embedding specimen - Google Patents
Method for manufacturing ornamental fish embedding specimen Download PDFInfo
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- CN110934130A CN110934130A CN201911226837.7A CN201911226837A CN110934130A CN 110934130 A CN110934130 A CN 110934130A CN 201911226837 A CN201911226837 A CN 201911226837A CN 110934130 A CN110934130 A CN 110934130A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
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Abstract
The embodiment of the invention discloses a method for manufacturing an ornamental fish embedding specimen, and particularly relates to the technical field of specimen manufacturing, which comprises the following specific steps: s1, processing the fish; s2, performing antiseptic treatment; s3, preparing a soaked specimen; s4, manufacturing an ecological landscape specimen; and S5, embedding. The invention is safe and nontoxic, has high environmental protection coefficient, has real and natural specimen shape, overcomes the problems of distortion, shriveling and white spots and bubbles caused by incomplete dehydration in the traditional fish specimen embedding process, is convenient and intuitive for the prepared fish specimen embedding, can be observed and learned at 360 degrees, overcomes the defect of inconvenient observation of the traditional specimen, simultaneously, the fish can swim and live in a soaking solution, preserves the shape in a natural state, can be preserved for a long time, is convenient to use and store, has stable specimen shape, can be used for a long time after being put into use once, and greatly reduces the maintenance and updating cost of the specimen.
Description
Technical Field
The embodiment of the invention relates to the technical field of specimen preparation, in particular to a preparation method of an ornamental fish embedding specimen.
Background
The specimen is an animal, plant, mineral, etc., and can be preserved for a long time and maintain the original appearance as much as possible after various treatments, thereby providing the specimen for exhibition, demonstration, education, identification, examination and other various researches. The specimen processing method may be performed by subjecting the whole individual (or even a plurality of individuals, such as bacteria, algae, or fungi, or other small and colonized individuals) or a part of the sample to physical air drying, vacuum, chemical preservative treatment, or the like. Specimens can be broadly classified into: animal specimens, bird specimens, fish specimens, insects, plant specimens, bone specimens, shrimp and crab specimens, fossil specimens, and the like.
The fish can make the infusion specimen, the fixation and preservation of the infusion specimen all adopt the chemical method, the whole process is carried on in the aqueous solution of the chemical agent, this method has wide range of application, various biological materials, especially the animal and plant material with large water content, including the human anatomy material can all adopt, the infusion specimen in the fixation and preservation process, the phenomena of discolouration, fading, deformation, mildewing, etc. that appear, all change the quality to different extent, there are several following conditions mainly; biochemical quality change; changing the biomass; the photochemical metamorphic pigment molecules form an electronic excited state after absorbing visible light, and the excited pigment molecules participate in a photo-oxidation reaction to cause the cracking of the pigment.
The preparation of the fish specimen in the prior art has the following defects: traditional specimen preparation needs chemical venom to soak, and the environmental protection coefficient is low to traditional method is because the dehydration is not thorough easily leads to the distortion, shrivelled, the problem that appears white spot and bubble for the specimen vividness of preparation is poor, is unfavorable for observing and studying.
Disclosure of Invention
Therefore, the embodiment of the invention provides a method for manufacturing an ornamental fish embedding specimen, which overcomes the defect that the traditional specimen needs to be soaked and stored by chemical venom, is safe, nontoxic, high in environmental protection coefficient, and real and natural in specimen form, solves the problems of distortion, shriveling, white spots and bubbles caused by incomplete dehydration in the traditional fish embedding specimen, is convenient and intuitive to manufacture, can be observed and learned at 360 degrees, overcomes the defect that the traditional specimen is inconvenient to observe, meanwhile, fishes swim in the soaking solution, are lifelike, preserve the form in the natural state, are thoroughly sterilized, can be preserved for a long time, are convenient to use and store, are stable in specimen form, can be used for a long time after being put into use once, and greatly reduce the maintenance and updating cost of the specimen.
In order to achieve the above object, the embodiments of the present invention provide the following technical solutions: a method for manufacturing an ornamental fish embedding specimen comprises the following specific steps:
s1, fish treatment:
s1.1, selecting materials: selecting fishes with complete fin rays and complete scales as specimen materials, and cleaning the fishes;
s1.2, disinfection: preparing a mercuric chloride solution and 95% alcohol into a mercuric chloride alcohol solution in proportion, soaking and disinfecting fish in the mercuric chloride alcohol solution, taking out the fish after soaking, preparing dichloroethane and carbon tetrachloride into a mixture in proportion, putting the fish into a closed environment, putting the mixture into the environment, fumigating the fish by gas emitted by the mixture to kill parasites and eggs on the fish body;
s2, antiseptic treatment:
s2.1, preparing an antiseptic solution: mixing sodium chloride, ethanol, glacial acetic acid, glycerol and citric acid according to the weight ratio of 5-8: 6-10: 1-3: 0.5-1: 1-2 to prepare an antiseptic solution;
s2.2, injecting preservative solution into the body from the belly of the fish by using an injector to fix internal organs and prevent the internal organs from rotting, and then soaking the fish in the preservative solution;
s3, preparation of a soaked specimen: fixing the soaked fish on a glass sheet, then placing the glass sheet into an organic glass bottle, and adding an antiseptic solution into the organic glass bottle, wherein the antiseptic solution submerges the fish;
s4, manufacturing an ecological landscape specimen:
s4.1, selecting materials: collecting robust plants, and removing impurities such as soil, dead leaves and the like on the plants;
s4.2, disinfection: in a closed environment of the cleaned plants, preparing a mixture of dichloroethane and carbon tetrachloride according to a proportion, putting the mixture into the environment, and fumigating and disinfecting the plants by gas emitted by the mixture;
s4.3, pressing: placing the plants between drying paper, pressing the plants by using a flatting machine, absorbing the pressed water by the drying paper, replacing the drying paper with new drying paper, pressing again, and repeating the pressing for 2-4 times;
s4.4, drying: drying the pressed plant in a drying box;
s5, embedding: uniformly stirring and mixing epoxy resin and a triethylene tetramine curing agent according to a proportion to prepare resin, injecting a proper amount of resin into a silica gel mold, then placing the fish immersion specimen and the ecological landscape specimen in the resin for 6-10 hours, injecting the resin again, completely embedding the fish immersion specimen and the ecological landscape specimen, and taking out after the resin is completely solidified.
Further, in step S1.2, the ratio of the mercuric chloride solution to the 95% alcohol is 1: 100, soaking the fish in mercuric chloride alcohol solution for 5-10 min.
Further, in the steps S1.2 and S4.2, the ratio of dichloroethane to carbon tetrachloride is 3:1, and the fumigating time is 1-2 days.
Further, in step S2.2 the fish is soaked in the preservative solution for 6-8 days.
Further, in step S3, the fixing method is to fix the fish by threading the long needle with the white thread and then threading the long needle from the chest and the tail base of the fish respectively close to the glass surface, and the fish is put into the plexiglas bottle and then the mouth of the plexiglas bottle is sealed with wax.
Further, in step S4.4, the drying temperature of the drying oven is 50-60 ℃, and the drying time is 2-3 hours.
Further, in step S4.5, the ratio of the epoxy resin to the triethylene tetramine curing agent is 3: and 1, standing for 10min after configuration so as to naturally eliminate bubbles.
The embodiment of the invention has the following advantages:
1. the preparation method of the invention is safe and nontoxic, overcomes the defect that the traditional specimen needs to be soaked and stored by chemical venom, has high environmental protection coefficient, is real and natural in specimen shape, and solves the problems of distortion, shriveling, white spots and bubbles caused by incomplete dehydration in the traditional fish specimen embedding process;
2. the fish embedding specimen manufactured by the invention is convenient and intuitive, can be observed and learned at 360 degrees, overcomes the defect that the traditional specimen is inconvenient to observe, meanwhile, the fish is swimming and lifelike in the soak solution, preserves the shape in a natural state, is thoroughly sterilized, can be preserved for a long time, is convenient to use and store, has a stable specimen shape, can be used for a long time after being thrown into one time, and greatly reduces the maintenance and updating cost of the specimen.
Detailed Description
The present invention is described in terms of particular embodiments, other advantages and features of the invention will become apparent to those skilled in the art from the following disclosure, and it is to be understood that the described embodiments are merely exemplary of the invention and that it is not intended to limit the invention to the particular embodiments disclosed. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
the invention provides a method for manufacturing an ornamental fish embedding specimen, which comprises the following specific steps:
s1, fish treatment:
s1.1, selecting materials: selecting fishes with complete fin rays and complete scales as specimen materials, and cleaning the fishes;
s1.2, disinfection: mixing mercuric chloride solution with 95% alcohol according to the weight ratio of 1: preparing mercuric chloride alcoholic solution according to the proportion of 100, soaking and disinfecting fish in the mercuric chloride alcoholic solution for 5min, taking out the fish, preparing dichloroethane and carbon tetrachloride into a mixture according to the proportion of 3:1, putting the fish into a closed environment, putting the mixture into the environment, and fumigating the fish for 1 day by using gas emitted from the mixture so as to kill parasites and eggs on the fish;
s2, antiseptic treatment:
s2.1, preparing an antiseptic solution: mixing sodium chloride, ethanol, glacial acetic acid, glycerol and citric acid according to the weight ratio of 5: 6: 1: 0.5: 1 to prepare an antiseptic solution;
s2.2, injecting preservative solution into the body from the belly of the fish by using an injector to fix internal organs and prevent the internal organs from rotting, and then soaking the fish in the preservative solution for 6 days;
s3, preparation of a soaked specimen: after the long needle is threaded with the white silk thread, the long needle respectively penetrates through the fish from the chest part and the tail base part of the fish close to one side of the glass surface, so that the soaked fish is fixed on a glass sheet and then is placed into an organic glass bottle, an antiseptic solution is added into the organic glass bottle, the antiseptic solution submerges the fish, and then the opening of the organic glass bottle is sealed by wax;
s4, manufacturing an ecological landscape specimen:
s4.1, selecting materials: collecting robust plants, and removing impurities such as soil, dead leaves and the like on the plants;
s4.2, disinfection: in a closed environment of the cleaned plants, preparing a mixture of dichloroethane and carbon tetrachloride according to a ratio of 3:1, putting the mixture into the environment, and fumigating and disinfecting the plants for 1 day by using gas emitted by the mixture;
s4.3, pressing: placing the plants between drying paper, pressing the plants by using a flatting machine, absorbing the pressed water by the drying paper, replacing the drying paper with new drying paper, pressing again, and repeating the pressing for 2 times;
s4.4, drying: drying the pressed plant in a drying oven at 50 deg.C for 2 hr;
s5, embedding: epoxy resin and triethylene tetramine curing agent are mixed according to the proportion of 3:1 proportion, stirring and mixing uniformly, standing for 10min after configuration to eliminate air bubbles naturally to prepare resin, injecting a proper amount of resin into a silica gel mold, then placing the fish soaked specimen and the ecological landscape specimen in the resin for 6 hours, injecting the resin again, embedding the fish soaked specimen and the ecological landscape specimen completely, and taking out after the resin is solidified completely.
Example 2:
the invention provides a method for manufacturing an ornamental fish embedding specimen, which comprises the following specific steps:
s1, fish treatment:
s1.1, selecting materials: selecting fishes with complete fin rays and complete scales as specimen materials, and cleaning the fishes;
s1.2, disinfection: mixing mercuric chloride solution with 95% alcohol according to the weight ratio of 1: preparing mercuric chloride alcoholic solution according to the proportion of 100, soaking and disinfecting fish in the mercuric chloride alcoholic solution for 7.5min, taking out, preparing dichloroethane and carbon tetrachloride into a mixture according to the proportion of 3:1, putting the fish into a closed environment, putting the mixture into the environment, fumigating the fish for 1.5 days by using gas emitted from the mixture so as to kill parasites and ova on the fish body;
s2, antiseptic treatment:
s2.1, preparing an antiseptic solution: mixing sodium chloride, ethanol, glacial acetic acid, glycerol and citric acid according to the weight ratio of 6.5: 8: 2: 0.75: 1.5 to prepare an antiseptic solution;
s2.2, injecting preservative solution into the body from the belly of the fish by using an injector to fix internal organs and prevent the internal organs from rotting, and then soaking the fish in the preservative solution for 7 days;
s3, preparation of a soaked specimen: after the long needle is threaded with the white silk thread, the long needle respectively penetrates through the fish from the chest part and the tail base part of the fish close to one side of the glass surface, so that the soaked fish is fixed on a glass sheet and then is placed into an organic glass bottle, an antiseptic solution is added into the organic glass bottle, the antiseptic solution submerges the fish, and then the opening of the organic glass bottle is sealed by wax;
s4, manufacturing an ecological landscape specimen:
s4.1, selecting materials: collecting robust plants, and removing impurities such as soil, dead leaves and the like on the plants;
s4.2, disinfection: in a closed environment of the cleaned plants, preparing a mixture of dichloroethane and carbon tetrachloride according to a ratio of 3:1, putting the mixture into the environment, and fumigating and disinfecting the plants for 1.5 days by using gas emitted by the mixture;
s4.3, pressing: placing the plants between drying paper, pressing the plants by using a flatting machine, absorbing the pressed water by the drying paper, replacing the drying paper with new drying paper, pressing again, and repeating the pressing for 3 times;
s4.4, drying: drying the pressed plant in a drying oven at 55 deg.C for 2.5 hr;
s5, embedding: epoxy resin and triethylene tetramine curing agent are mixed according to the proportion of 3:1 proportion, stirring and mixing uniformly, standing for 10min after configuration to eliminate air bubbles naturally to prepare resin, injecting a proper amount of resin into a silica gel mold, then placing the fish soaked specimen and the ecological landscape specimen in the resin for 8 hours, injecting the resin again, embedding the fish soaked specimen and the ecological landscape specimen completely, and taking out after the resin is solidified completely.
Example 3:
the invention provides a method for manufacturing an ornamental fish embedding specimen, which comprises the following specific steps:
s1, fish treatment:
s1.1, selecting materials: selecting fishes with complete fin rays and complete scales as specimen materials, and cleaning the fishes;
s1.2, disinfection: mixing mercuric chloride solution with 95% alcohol according to the weight ratio of 1: preparing mercuric chloride alcoholic solution according to the proportion of 100, soaking and disinfecting fish in the mercuric chloride alcoholic solution for 10min, taking out the fish, preparing dichloroethane and carbon tetrachloride into a mixture according to the proportion of 3:1, putting the fish into a closed environment, putting the mixture into the environment, and fumigating the fish for 2 days by using gas emitted from the mixture so as to kill parasites and eggs on the fish;
s2, antiseptic treatment:
s2.1, preparing an antiseptic solution: mixing sodium chloride, ethanol, glacial acetic acid, glycerol and citric acid according to the weight ratio of 8: 10: 3: 1: 2 to prepare an antiseptic solution;
s2.2, injecting preservative solution into the body from the belly of the fish by using an injector to fix internal organs and prevent the internal organs from rotting, and then soaking the fish in the preservative solution for 8 days;
s3, preparation of a soaked specimen: after the long needle is threaded with the white silk thread, the long needle respectively penetrates through the fish from the chest part and the tail base part of the fish close to one side of the glass surface, so that the soaked fish is fixed on a glass sheet and then is placed into an organic glass bottle, an antiseptic solution is added into the organic glass bottle, the antiseptic solution submerges the fish, and then the opening of the organic glass bottle is sealed by wax;
s4, manufacturing an ecological landscape specimen:
s4.1, selecting materials: collecting robust plants, and removing impurities such as soil, dead leaves and the like on the plants;
s4.2, disinfection: in a closed environment of the cleaned plants, preparing a mixture of dichloroethane and carbon tetrachloride according to a ratio of 3:1, putting the mixture into the environment, and fumigating and disinfecting the plants for 2 days by using gas emitted by the mixture;
s4.3, pressing: placing the plants between drying paper, pressing the plants by using a flatting machine, absorbing the pressed water by the drying paper, replacing the drying paper with new drying paper, pressing again, and repeating the pressing for 4 times;
s4.4, drying: drying the pressed plant in a drying oven at 60 deg.C for 3 hr;
s5, embedding: epoxy resin and triethylene tetramine curing agent are mixed according to the proportion of 3:1 proportion, stirring and mixing uniformly, standing for 10min after configuration to eliminate air bubbles naturally to prepare resin, injecting a proper amount of resin into a silica gel mold, then placing the fish soaked specimen and the ecological landscape specimen in the resin for 10 hours, injecting the resin again, embedding the fish soaked specimen and the ecological landscape specimen completely, and taking out after the resin is solidified completely.
Example 4:
90 embedded specimens were prepared by the preparation method of each of examples 1 to 3, and the following data were obtained for each 30 embedded specimens:
as can be seen from the above table, the preparation method in embodiment 2 is suitable, the prepared specimen has the best disinfection effect, and the quality change time of the specimen is effectively prolonged, so that the storage time of the specimen is effectively prolonged, the use and the storage are convenient, the specimen can be used for a long time after being put into use once, and the maintenance and updating costs of the specimen are greatly reduced.
Although the invention has been described in detail above with reference to a general description and specific examples, it will be apparent to one skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (7)
1. A method for manufacturing an ornamental fish embedding specimen is characterized by comprising the following steps: the method comprises the following specific steps:
s1, fish treatment:
s1.1, selecting materials: selecting fishes with complete fin rays and complete scales as specimen materials, and cleaning the fishes;
s1.2, disinfection: preparing a mercuric chloride solution and 95% alcohol into a mercuric chloride alcohol solution in proportion, soaking and disinfecting fish in the mercuric chloride alcohol solution, taking out the fish after soaking, preparing dichloroethane and carbon tetrachloride into a mixture in proportion, putting the fish into a closed environment, putting the mixture into the environment, fumigating the fish by gas emitted by the mixture to kill parasites and eggs on the fish body;
s2, antiseptic treatment:
s2.1, preparing an antiseptic solution: mixing sodium chloride, ethanol, glacial acetic acid, glycerol and citric acid according to the weight ratio of 5-8: 6-10: 1-3: 0.5-1: 1-2 to prepare an antiseptic solution;
s2.2, injecting preservative solution into the body from the belly of the fish by using an injector to fix internal organs and prevent the internal organs from rotting, and then soaking the fish in the preservative solution;
s3, preparation of a soaked specimen: fixing the soaked fish on a glass sheet, then placing the glass sheet into an organic glass bottle, and adding an antiseptic solution into the organic glass bottle, wherein the antiseptic solution submerges the fish;
s4, manufacturing an ecological landscape specimen:
s4.1, selecting materials: collecting robust plants, and removing impurities such as soil, dead leaves and the like on the plants;
s4.2, disinfection: in a closed environment of the cleaned plants, preparing a mixture of dichloroethane and carbon tetrachloride according to a proportion, putting the mixture into the environment, and fumigating and disinfecting the plants by gas emitted by the mixture;
s4.3, pressing: placing the plants between drying paper, pressing the plants by using a flatting machine, absorbing the pressed water by the drying paper, replacing the drying paper with new drying paper, pressing again, and repeating the pressing for 2-4 times;
s4.4, drying: drying the pressed plant in a drying box;
s5, embedding: uniformly stirring and mixing epoxy resin and a triethylene tetramine curing agent according to a proportion to prepare resin, injecting a proper amount of resin into a silica gel mold, then placing the fish immersion specimen and the ecological landscape specimen in the resin for 6-10 hours, injecting the resin again, completely embedding the fish immersion specimen and the ecological landscape specimen, and taking out after the resin is completely solidified.
2. The method for manufacturing an ornamental fish embedding specimen according to claim 1, characterized in that: in step S1.2, the ratio of the mercuric chloride solution to the 95% alcohol is 1: 100, soaking the fish in mercuric chloride alcohol solution for 5-10 min.
3. The method for manufacturing an ornamental fish embedding specimen according to claim 1, characterized in that: in the steps S1.2 and S4.2, the ratio of dichloroethane to carbon tetrachloride is 3:1, and the fumigating time is 1-2 days.
4. The method for manufacturing an ornamental fish embedding specimen according to claim 1, characterized in that: in step S2.2 the fish is soaked in the preservative solution for 6-8 days.
5. The method for manufacturing an ornamental fish embedding specimen according to claim 1, characterized in that: in the step S3, the fixing method is to fix the fish by threading the long needle with the white thread and then threading the long needle from the chest and the tail base of the fish respectively close to the glass surface, and the fish is put into the organic glass bottle and then the opening of the organic glass bottle is sealed by wax.
6. The method for manufacturing an ornamental fish embedding specimen according to claim 1, characterized in that: in step S4.4, the drying temperature of the drying oven is 50-60 ℃, and the drying time is 2-3 hours.
7. The method for manufacturing an ornamental fish embedding specimen according to claim 1, characterized in that: in step S4.5, the preparation ratio of the epoxy resin to the triethylene tetramine curing agent is 3: and 1, standing for 10min after configuration so as to naturally eliminate bubbles.
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| CN111572937A (en) * | 2020-05-07 | 2020-08-25 | 重庆市中药研究院 | Novel wine bottle processing and manufacturing method based on embedding technology |
| CN113491266A (en) * | 2020-04-02 | 2021-10-12 | 山东省海洋生物研究院 | Method for preparing fish specimen |
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