CN108207929A - A kind of store method of zoobenthos sample - Google Patents
A kind of store method of zoobenthos sample Download PDFInfo
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- CN108207929A CN108207929A CN201810120213.6A CN201810120213A CN108207929A CN 108207929 A CN108207929 A CN 108207929A CN 201810120213 A CN201810120213 A CN 201810120213A CN 108207929 A CN108207929 A CN 108207929A
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- zoobenthos
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- firming agent
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
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Abstract
The invention discloses a kind of store methods of zoobenthos sample, include the following steps：(1) mud sample containing zoobenthos of acquisition by sub-sieve is sieved, is rinsed well with seawater, dispensed after sorting repeatedly；(2) zoobenthos of packing will be handled through step (1) first with formalin soaking sterilization, then impregnates in distilled water, is washed again with distilled water after being washed with alcoholic solution；(3) zoobenthos handled through step (2) addition firming agent for specimen is fixed；(4) zoobenthos sample is obtained to addition protective agent above the firming agent for specimen of step (3).The zoobenthos sample that the present invention makes can protect that color is fresh-keeping, and color and form remain unchanged within 2 years for a long time, and the sample made is integrally rendered as solid-state, has the characteristics of nontoxic, easy moulding, convenient transportation, have larger application value.
Description
Technical field
The invention belongs to Saving specimen technical fields, and in particular to a kind of store method of zoobenthos sample.
Background technology
Zoobenthos refers to live in the invertebrate of water bottom, and deposition is attached to including water body, deposit neutralization
All animals on object surface, type is various, more than 1,000,000 kinds.Zoobenthos because with quantity it is more, it is widely distributed, to environment change
Change sensitivity, life cycle length, migrate the features such as ability is weaker frequently as environmental monitoring index.Zoobenthos is in third nutrition
Grade has important role for ecosystem substance cycle and energy flow.As the research in terms of zoobenthos increasingly increases
It sums it up deeply, the collection and preservation of zoobenthos sample seem significant for research in this respect.Also, zoobenthos mark
Originally it is the important material of biological subject, is the important models of school instruction and the important carrier of Science communication.Therefore, the bottom of by
The animal that dwells is fabricated to sample with important teaching and scientific research meaning.
At present, fixed and preservation zoobenthos sample most often utilizes formalin (i.e. formalin) and ethyl alcohol
Solution, but formalin mainly has there are many defects：(1) formaldehyde easily makes sample body harden, deform, fade, not protect
It is fresh；(2) in formalin after soaking time length, zoobenthos is oxidized easily, and leads to its decomposition or corruption, loses guarantor
Deposit meaning；(3) molluscan caliche, can be corroded by acid formaldehyde, when being fixed with formaldehyde, must add in a small amount of soda or
In borax and acid formaldehyde；(4) it need to often be replaced in formalin because easily polymerizeing generation precipitation containing methanol；(3) methanol toxicity
Greatly, there is strong impulse smell, volatility is stronger, not only pollutes environment, and teratogenesis is carcinogenic with obvious effects, can also different journeys
The eyes and respiratory system of the injury operator of degree, when it, concentration reaches 30mg/m in air3When, understand causing death immediately,
It contacts for a long time, it is larger to health effect；(5) the preservation liquid and containing ethyl alcohol also can make sample body harden after being used for a long time,
Discolouration phenomena becomes apparent from than formaldehyde；And there is the defects of being not easy moulding, transport trouble in the sample that both solution preserve.
To solve the above problems, also the new specimen fluids of someone's Selecting research preserve sample, and achieve good
Effect.Patent 98108129.0 discloses a kind of preservation liquid, the preservation liquid at least by a kind of sodium salt or the preservative of sylvite class, extremely
A kind of few polyhydroxy base class color preserving agent and water composition；Chinese patent CN88105221A discloses a kind of biological sample and protects color preservative
Preparation method, the preservative that this method is prepared use compound prescription, the substances such as ethyl alcohol, potassium acetate are made mixed liquor in proportion,
The preservative can keep constant primary colors, soft fresh, the not atrophy of body, it is indeformable the features such as.But in above-mentioned two patent
It is all liquid to preserve liquid or preservative, it has not been convenient to sample moulding, and the sample that preserves is yielding and transport trouble.Invention is special
Sharp 103601844 B of CN disclose the preparation method that a kind of zoological specimens preserve gel, this gel is 1%-10% by mass ratio
Acrylamide compound, 15%-30% Tris-HCl, 0.1%-0.5% N, N- methylene-bisacrylamides, 5%-
The PH that the water of 20% lauryl sodium sulfate, the ammonium persulfate of 0.5%-1.0% and 38.5%-78.5% is formed is
3.5-5.0 solution be made.PH is the growth that 3.5-5.0 effectively inhibits animals and plants bacterium and mould.The invention
Zoological specimens preserve gel have it is good preserve, be fixed, sterilization, antisepsis, make the good product of animal or plant specimen and
It is readily transported.But the invention adds a variety of harmful chemical reagent in preparation process, and by the invention system
Standby gel has stronger acidity, unfavorable to the preservation of the zoobenthos with minerals shell.Therefore, it still needs to seek one kind more
Suitable for preserving the store method of zoobenthos sample.
Invention content
In view of the deficiencies of the prior art, the present invention provides a kind of store method of zoobenthos sample, this method can
Zoobenthos sample is made to protect for a long time, and color is fresh-keeping, form is constant, and the store method of the biological sample of the present invention is preserved for on-liquid,
Convenient transportation.
To achieve the above object, the technical scheme is that a kind of store method of zoobenthos sample, including following
Step：
(1) mud sample containing zoobenthos of acquisition by sub-sieve is sieved, is rinsed well repeatedly with seawater, after sorting
It is dispensed；
(2) zoobenthos of packing will be handled through step (1) first with formalin soaking sterilization, will then be soaked in distilled water
Bubble, is washed after being washed with alcoholic solution with distilled water again；
(3) zoobenthos handled through step (2) addition firming agent for specimen is fixed；
(4) zoobenthos sample is obtained to addition protective agent above the firming agent for specimen of step (3).
Preferably, in step (1), sorting is one or more in writing brush, pincet, dissecting needle with tool.
Further, in step (2), before with formalin soaking sterilization, zoobenthos is first placed in culture dish, is added
Enter 3-5ml seawater, 95% alcohol then is added dropwise every 10min, until zoobenthos body line up is anaesthetized.
Preferably, in step (2), a concentration of 50-55wt% of the formalin, soaking time 30-40s.
Preferably, in step (2), the soaking time in distilled water is 5-6min.
Preferably, in step (2), the volumetric concentration 75% of the alcohol, ethanol wash number is 2-3 times, each 5-8s.
Preferably, it is 2-3 times with the number that distilled water is washed in step (2), each time is 10-15s.
Further, in step (3), the phosphate buffer containing 0.1mol/L in the firming agent for specimen, 0.001%
Glacial acetic acid, the gelatin of 10-15%, 3-5% chitosan, surplus is water；The pH of the firming agent for specimen is 6-6.5.
Preferably, in step (3), the N- deacetylations of the chitosan are 70-75%.
Preferably, in step (3), the fixation procedure of zoobenthos includes the following steps：
A, in the zoobenthos merging sample fixed cup that will be handled through step (2), the mark of zoobenthos is fixed with fixture
This position and specimen morphology；
B, ready chitosan, glacial acetic acid and part water are poured into clean container, being sufficiently stirred makes chitosan complete
Fully dissolved；Gelatin and part water are added in into container, is heated while stirring, until solution boiling stops heating, treats that gelatin is complete
Continue to stir 1min after dissolving；
C, phosphate buffer is added in into container, the firming agent for specimen is sufficiently stirred to obtain after adding water trim；
D, it treats that the firming agent for specimen temperature in step C containers is down to 65 DEG C, the sample of from firming agent for specimen to step A is fixed
It is poured slowly into cup, side by side bubble removing；
E, the sample fixed cup for treating step D is placed in 0-4 DEG C of environment to solidify down toward firming agent for specimen；
F, fixture is taken out.
Preferably, in step (4), the protective agent is grouped as by each group of following weight percent：35% gelatin, 4%
Allicin, 10% glacial acetic acid, surplus are water；Protectant height is 0.5-2cm.
Preferably, in step (4), protectant preparation process is：Gelatin is weighed by proportioning to be added to the water, at 90 DEG C
Then fully dissolving adds in glacial acetic acid and is uniformly mixed, be cooled to 60 DEG C and add allicin and be uniformly mixed；It will be still in liquid state
The protective agent of state is poured slowly into the top of the firming agent for specimen solidified, and 0-4 DEG C of environment solidifies completely down toward protective agent.
With prior art ratio, advantageous effects of the invention：
(1) present invention seals zoobenthos by selecting appropriate component proportion that a kind of transparent gel can be made
It deposits, not only overcomes the caused disadvantages mentioned above of formaldehyde and alcohols specimen preserving liquid, but also there is better preservation effect,
And the composition end-state is solid gel, saves the moisture and state of sample well.
(2) gel that the present invention is formed has figurate effect to animal, and compared to liquid preservative liquid, gel is limpid
It is transparent, it has no irritating odor, it is also very convenient compared to the fixed sample transport of liquid.
(3) chitosan added in configuration process of the present invention not only has preferable antibacterial activity, inhibits some fungies, thin
Bacterium and the growth and breeding of virus extend the pot-life of sample, additionally it is possible to and it is crosslinked with gelatin, forms stable gel,
Cushioning effect is good with fixed effect, unless by violent shock, is not easily broken or crushes.
(4) present invention is fitted closely using protective agent with firming agent for specimen gel, can completely cut off the oxidation of air；
Also, high mechanical strength tough and tensile transparent by the colloid that protective agent is formed can further reduce physics or chemical action to mark
The influence of this fixative gel；And the bacteriostasis of allicin and the extremely strong work(of antibacterial and penetration power of glacial acetic acid is utilized
Effect, with reference to the duplicate protection of firming agent for specimen gel, after preserving 2 years, color and luster, shape of zoobenthos sample etc. do not change
Become.
(5) present invention dwells to the bottom with minerals shell for fixing the pH of latex gel partial neutral of zoobenthos sample
The no any harmful effect of preservation of object, and the component for being used to prepare firming agent for specimen gel is mostly mild chemical reagent or day
Right substance will not cause chemical erosion to sample, and the completion and appearance for helping to ensure that sample remain unchanged.
(6) present invention sterilized using chitosan and allicin, anti-corrosion, and protective agent in firming agent for specimen with selecting
Be nonpoisonous chemicla and natural materials, have no toxic side effect to human body and environment, be make zoobenthos sample select very well,
It can be widely applied in preparation of specimen's work in the scientific popularizations place such as museum, school.
Summary, it is fresh-keeping that the zoobenthos sample that makes of the present invention can protect color for a long time, color and form within 2 years
It remains unchanged, and the sample made is integrally rendered as solid-state, has the characteristics of nontoxic, easy moulding, convenient transportation, has larger
Application value.
Description of the drawings
Fig. 1 is the schematic diagram of the embodiment of the present invention 1；
Fig. 2 is the schematic diagram of the embodiment of the present invention 2；
Fig. 3 is the schematic diagram of the embodiment of the present invention 3.
Specific embodiment
The present invention will be further described in detail with reference to the specific embodiments.
Embodiment 1
A kind of store method of zoobenthos sample, includes the following steps：
(1) sample adopted on the spot or is taken back into indoor carry out sorting.The mud sample grabbed in bucket is poured into mistake in sub-sieve
Sieve rinses (or by the sea in water sieve and wash) in the seawater, until sludge is cleaned completely, dregs then are poured into white dissecting pan
It is interior, limpid seawater is added in, rinses fixed preservation in the sealed plastic bottle several times, being put into repeatedly.It is remained in sample sifter and dissecting pan
Remaining sample is sorted Bohai Sea lattice squama worm with writing brush, to avoid the soft polypide of injury.By the Bohai Sea lattice squama worm after sorting
It is dispensed and is marked with label.Bohai Sea lattice squama worm before next step operation is carried out should first be anaesthetized, it is made to unfold again solid
It is fixed.Specific practice adds a small amount of seawater, then 3ml seawater was added dropwise every 10 minutes for benthon is placed in culture dish
95% alcohol, until polypide all stretches thorough anesthesia.
(2) Bohai Sea lattice squama worm for handling packing through step (1) first is impregnated 30s with the formalin of 50wt% to kill
Bacterium, the formaldehyde of high concentration so that Bohai Sea lattice squama worm is dead rapidly or even stiff property is dead, convenient for later observation, while also can
Complete the sterilization operation to sample.Then 5min is impregnated in distilled water, washs 2 times with 75% (v/v) alcoholic solution, every time
5s, then washing 2 times is carried out with distilled water, each time is 10s.
(3) Bohai Sea lattice squama worm handled through step (2) is added in firming agent for specimen to be fixed.
Phosphate buffer containing 0.1mol/L in the firming agent for specimen, 0.001% glacial acetic acid, 10% it is bright
Glue, 3% chitosan, surplus are water；The pH of the firming agent for specimen is 6.The N- deacetylations of the chitosan are 70%.
The fixation procedure of Bohai Sea lattice squama worm includes the following steps：
A, in the Bohai Sea lattice squama worm merging sample fixed cup that will be handled through step (2), Bohai Sea lattice squama worm is fixed with fixture
Specimen location and specimen morphology；
B, ready chitosan, glacial acetic acid and part water are poured into clean container, being sufficiently stirred makes chitosan complete
Fully dissolved；Gelatin and part water are added in into container, is heated while stirring, until solution boiling stops heating, treats that gelatin is complete
Continue to stir 1min after dissolving；
C, phosphate buffer is added in into container, the firming agent for specimen is sufficiently stirred to obtain after adding water trim；
D, it treats that the firming agent for specimen temperature in step C containers is down to 65 DEG C, the sample of from firming agent for specimen to step A is fixed
It is poured slowly into cup, side by side bubble removing；
E, the sample fixed cup for treating step D is placed in 0 DEG C of environment to solidify down toward firming agent for specimen；
F, fixture is taken out.
(4) protective agent is added in into step (3) and obtains zoobenthos sample.
The protective agent is grouped as by each group of following weight percent：35% gelatin, 4% allicin, 10% glacial acetic acid,
Surplus is water；Protectant height is 0.5cm.Protectant preparation process is：Gelatin, which is weighed, by proportioning adds in water
In, it is fully dissolved at 90 DEG C, then adds in glacial acetic acid and be uniformly mixed, be cooled to 60 DEG C and add allicin and be uniformly mixed.It will still
Protective agent in liquid condition is poured slowly into the top of the firming agent for specimen solidified, and 0 DEG C of environment is completely solidifying down toward protective agent
Gu obtain Bohai Sea lattice squama worm sample finished product.
Respectively the Bohai Sea lattice squama worm sample preparation after 1 month, 3 months, 6 months, 12 months, 24 months, 48 months it is right
Sample is observed, it is found that state, color and luster, the shape nothing of Bohai Sea lattice squama worm are substantially change.48th month after sample preparation,
Bohai Sea lattice squama worm is taken out and takes out observation after rinsing, finds the state of Bohai Sea lattice squama worm, color and luster, shape without any change, it
After place it in normal ambient environments, after 2 days observation find Bohai Sea lattice squama worm just started to decay it is rotten.
Embodiment 2
A kind of store method of zoobenthos sample, includes the following steps：
(1) sample adopted on the spot or is taken back into indoor carry out sorting.The mud sample grabbed in bucket is poured into mistake in sub-sieve
Sieve rinses (or by the sea in water sieve and wash) in the seawater, until sludge is cleaned completely, dregs then are poured into white dissecting pan
It is interior, limpid seawater is added in, rinses fixed preservation in the sealed plastic bottle several times, being put into repeatedly.It is remained in sample sifter and dissecting pan
Walkingstick after sorting is dispensed and is marked with label to walkingstick pick by remaining sample pincet or dissecting needle.It will
Walkingstick should first be anaesthetized before next step operation is carried out, and is unfolded it and is fixed again.Specific practice is that walkingstick is placed on training
It supports in ware, adds a small amount of seawater, then 95% alcohol was added dropwise every 10 minutes in 5ml seawater, until polypide all stretches thoroughly
Anesthesia.
(2) walkingstick for handling packing through step (1) first is impregnated 40s with the formalin of 55wt% to sterilize, it is high
The formaldehyde of concentration so that walkingstick is dead rapidly or even stiff property is dead, convenient for later observation, while can also complete to sample
Sterilization operation.Then 6min is impregnated in distilled water, is washed 3 times, each 8s with 75% (v/v) alcoholic solution, then is steamed with double
Water carries out washing 3 times, and each time is 15s.
(3) walkingstick handled through step (2) addition firming agent for specimen is fixed.
Phosphate buffer containing 0.1mol/L in the firming agent for specimen, 0.001% glacial acetic acid, 10-15%
The chitosan of gelatin, 3-5%, surplus are water；The pH of the firming agent for specimen is 6.5.The N- deacetylations of the chitosan are
75%.
The fixation procedure of walkingstick includes the following steps：
A, in the walkingstick merging sample fixed cup that will be handled through step (2), the sample position of walkingstick is fixed with fixture
It puts and specimen morphology；
B, ready chitosan, glacial acetic acid and part water are poured into clean container, being sufficiently stirred makes chitosan complete
Fully dissolved；Gelatin and part water are added in into container, is heated while stirring, until solution boiling stops heating, treats that gelatin is complete
Continue to stir 1min after dissolving；
C, phosphate buffer is added in into container, the firming agent for specimen is sufficiently stirred to obtain after adding water trim；
D, it treats that the firming agent for specimen temperature in step C containers is down to 65 DEG C, the sample of from firming agent for specimen to step A is fixed
It is poured slowly into cup, side by side bubble removing；
E, the sample fixed cup for treating step D is placed in 4 DEG C of environment to solidify down toward firming agent for specimen；
F, fixture is taken out.
(4) protective agent is added in into step (3) and obtains zoobenthos sample.
The protective agent is grouped as by each group of following weight percent：35% gelatin, 4% allicin, 10% glacial acetic acid,
Surplus is water；Protectant height is 2cm.Protectant preparation process is：Gelatin is weighed by proportioning to be added to the water,
It is fully dissolved at 90 DEG C, then adds in glacial acetic acid and be uniformly mixed, be cooled to 60 DEG C and add allicin and be uniformly mixed.It will be still in
The protective agent of liquid condition is poured slowly into the top of the firming agent for specimen solidified, and 4 DEG C of environment solidify i.e. completely down toward protective agent
Obtain walkingstick sample finished product.
Respectively 1 month after the preparation of walkingstick sample, 3 months, 6 months, 12 months, 24 months, 48 months to sample
It is observed, it is found that state, color and luster, the shape nothing of walkingstick are substantially change.48th month after sample preparation, by walkingstick
It takes out and observation is taken out after rinsing, it is found that state, color and luster, the shape of walkingstick without any change, place it in normal room later
In interior environment, observation discovery walkingstick has just started to decay rotten after 2.5 days.
Embodiment 3
A kind of store method of zoobenthos sample, includes the following steps：
(1) sample adopted on the spot or is taken back into indoor carry out sorting.The mud sample grabbed in bucket is poured into mistake in sub-sieve
Sieve rinses (or by the sea in water sieve and wash) in the seawater, until sludge is cleaned completely, dregs then are poured into white dissecting pan
It is interior, limpid seawater is added in, rinses fixed preservation in the sealed plastic bottle several times, being put into repeatedly.It is remained in sample sifter and dissecting pan
Remaining sample carries out pick with pincet to ridge abdomen brown shrimp, and the ridge abdomen brown shrimp after go-no-go is dispensed and is marked with label.
Ridge abdomen brown shrimp before next step operation is carried out should be anaesthetized first, unfold it and fix again.Specific practice is will
Ridge abdomen brown shrimp is placed in culture dish, adds a small amount of seawater, and then 95% alcohol was added dropwise every 10 minutes in 5ml seawater, until worm
Body all stretches thorough anesthesia.
(2) the ridge abdomen brown shrimp for handling packing through step (1) first is impregnated 35s with the formalin of 50wt% to sterilize,
The formaldehyde of high concentration so that ridge abdomen brown shrimp is dead rapidly or even stiff property is dead, convenient for later observation, while can also complete pair
The sterilization operation of sample.Then 5min is impregnated in distilled water, is washed 2 times, each 5s with 75% (v/v) alcoholic solution, then use
Distilled water carries out washing 2 times, and each time is 10s.
(3) the ridge abdomen brown shrimp handled through step (2) addition firming agent for specimen is fixed.
Phosphate buffer containing 0.1mol/L in the firming agent for specimen, 0.001% glacial acetic acid, 12% it is bright
The chitosan of glue, 3-5%, surplus are water；The pH of the firming agent for specimen is 6.5.The N- deacetylations of the chitosan are
75%.
The fixation procedure of ridge abdomen brown shrimp includes the following steps：
A, in the ridge abdomen brown shrimp merging sample fixed cup that will be handled through step (2), the mark of ridge abdomen brown shrimp is fixed with fixture
This position and specimen morphology；
B, ready chitosan, glacial acetic acid and part water are poured into clean container, being sufficiently stirred makes chitosan complete
Fully dissolved；Gelatin and part water are added in into container, is heated while stirring, until solution boiling stops heating, treats that gelatin is complete
Continue to stir 1min after dissolving；
C, phosphate buffer is added in into container, the firming agent for specimen is sufficiently stirred to obtain after adding water trim；
D, it treats that the firming agent for specimen temperature in step C containers is down to 65 DEG C, the sample of from firming agent for specimen to step A is fixed
It is poured slowly into cup, side by side bubble removing；
E, the sample fixed cup for treating step D is placed in 4 DEG C of environment to solidify down toward firming agent for specimen；
F, fixture is taken out.
(4) protective agent is added in into step (3) and obtains zoobenthos sample.
The protective agent is grouped as by each group of following weight percent：35% gelatin, 4% allicin, 10% glacial acetic acid,
Surplus is water；Protectant height is 2cm.Protectant preparation process is：Gelatin is weighed by proportioning to be added to the water,
It is fully dissolved at 90 DEG C, then adds in glacial acetic acid and be uniformly mixed, be cooled to 60 DEG C and add allicin and be uniformly mixed.It will be still in
The protective agent of liquid condition is poured slowly into the top of the firming agent for specimen solidified, and 4 DEG C of environment solidify i.e. completely down toward protective agent
Obtain ridge abdomen brown shrimp sample finished product.
Respectively ridge abdomen brown shrimp sample preparation after 1 month, 3 months, 6 months, 12 months, 24 months, 48 months to mark
This is observed, it is found that state, color and luster, the shape nothing of ridge abdomen brown shrimp are substantially change.48th month after sample preparation, by ridge
Abdomen brown shrimp takes out observation after taking out flushing, it is found that state, color and luster, the shape of ridge abdomen brown shrimp without any change, are put later
In normal ambient environments, observation discovery ridge abdomen brown shrimp has just started to decay rotten after 2 days.
Although above having used general explanation and specific embodiment, the present invention is described in detail, at this
On the basis of invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Therefore,
These modifications or improvements without departing from theon the basis of the spirit of the present invention belong to the scope of protection of present invention.
Claims (10)
1. a kind of store method of zoobenthos sample, which is characterized in that include the following steps：
(1) mud sample containing zoobenthos of acquisition by sub-sieve is sieved, is rinsed well with seawater, carried out after sorting repeatedly
Packing；
(2) zoobenthos of packing will be handled through step (1) first with formalin soaking sterilization, will then be impregnated in distilled water,
It is washed again with distilled water after being washed with alcoholic solution；
(3) zoobenthos handled through step (2) addition firming agent for specimen is fixed；
(4) protective agent is added in above the firming agent for specimen into step (3) and obtains zoobenthos sample.
2. the store method of zoobenthos sample according to claim 1, it is characterised in that：In step (2), with formaldehyde
Before solution soaking sterilization, zoobenthos is first placed in culture dish, adds in 3-5ml seawater, is then added dropwise 95% every 10min
Alcohol, until zoobenthos body line up is anaesthetized.
3. the store method of zoobenthos sample according to claim 1, it is characterised in that：In step (2), the formaldehyde
A concentration of 50-55wt% of solution, soaking time 30-40s；Soaking time in distilled water is 5-6min.
4. the store method of zoobenthos sample according to claim 1, it is characterised in that：In step (2), the alcohol
Volumetric concentration 75%, ethanol wash number be 2-3 times, each 5-8s.
5. the store method of zoobenthos sample according to claim 1, it is characterised in that：In step (2), distilled water is used
The number washed is 2-3 times, and each time is 10-15s.
6. the store method of zoobenthos sample according to claim 1, it is characterised in that：In step (3), the mark
Phosphate buffer containing 0.1mol/L in this fixative, 0.001% glacial acetic acid, the gelatin of 10-15%, 3-5% shell gather
Sugar, surplus are water；The pH of the firming agent for specimen is 6-6.5.
7. the store method of zoobenthos sample according to claim 6, it is characterised in that：In step (3), the shell gathers
The N- deacetylations of sugar are 70-75%.
8. the store method of zoobenthos sample according to claim 6, it is characterised in that：In step (3), zoobenthos
Fixation procedure include the following steps：
A, in the zoobenthos merging sample fixed cup that will be handled through step (2), the sample position of zoobenthos is fixed with fixture
It puts and specimen morphology；
B, ready chitosan, glacial acetic acid and part water are poured into clean container, being sufficiently stirred makes chitosan completely molten
Solution；Gelatin and part water are added in into container, is heated while stirring, until solution boiling stops heating, treats that gelatin is completely dissolved
After continue stir 1min；
C, phosphate buffer is added in into container, the firming agent for specimen is sufficiently stirred to obtain after adding water trim；
D, treat that the firming agent for specimen temperature in step C containers is down to 65 DEG C, by firming agent for specimen into the sample fixed cup of step A
It is poured slowly into, side by side bubble removing；
E, the sample fixed cup for treating step D is placed in 0-4 DEG C of environment to solidify down toward firming agent for specimen；
F, fixture is taken out.
9. the store method of zoobenthos sample according to claim 8, it is characterised in that：In step (4), the protection
Agent is grouped as by each group of following weight percent：35% gelatin, 4% allicin, 10% glacial acetic acid, surplus are water；The guarantor
The height for protecting agent is 0.5-2cm.
10. the store method of zoobenthos sample according to claim 9, it is characterised in that：In step (4), the guarantor
Shield agent preparation process be：Gelatin is weighed by proportioning to be added to the water, is fully dissolved at 90 DEG C, and it is equal then to add in glacial acetic acid mixing
It is even, it is cooled to 60 DEG C and adds allicin and be uniformly mixed；The mark solidified will be poured slowly into still in the protective agent of liquid condition
The top of this fixative, 0-4 DEG C of environment solidify completely down toward protective agent.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810120213.6A CN108207929A (en) | 2018-02-06 | 2018-02-06 | A kind of store method of zoobenthos sample |
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| CN201810120213.6A CN108207929A (en) | 2018-02-06 | 2018-02-06 | A kind of store method of zoobenthos sample |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109497035A (en) * | 2018-11-19 | 2019-03-22 | 张昌盛 | Bone and bone derived tissues sample preservation protective agent and preparation method |
| CN110476949A (en) * | 2019-08-22 | 2019-11-22 | 云南省热带作物科学研究所 | A kind of Slide processing of Africangiant snail |
| CN110663678A (en) * | 2019-10-16 | 2020-01-10 | 广西师范大学 | Method for manufacturing amphibious specimen |
| CN110692626A (en) * | 2019-11-21 | 2020-01-17 | 钦州市中医药研究所 | Dry plant specimen and preparation method thereof |
| CN113142183A (en) * | 2021-02-23 | 2021-07-23 | 台州学院 | System and method for manufacturing ecological specimens of verruca virginiana |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1887961A (en) * | 2006-07-28 | 2007-01-03 | 重庆师范大学 | Small biological sample preparing process with transparant water soluble resin |
| CN101636649A (en) * | 2007-02-27 | 2010-01-27 | 恰根有限公司 | Fixation of a biological material |
| CN103013437A (en) * | 2012-12-18 | 2013-04-03 | 中国人民解放军第三军医大学第一附属医院 | Interior label stationary liquid for biological specimen preserved through formalin, preparation method and application thereof |
| CN103583509A (en) * | 2013-11-27 | 2014-02-19 | 青岛海洋科技馆 | Making method of gel specimen |
| CN104585237A (en) * | 2015-02-11 | 2015-05-06 | 广东海洋大学 | Environmentally-friendly composite anti-mildew and antiseptic agent and application thereof |
| CN105994249A (en) * | 2016-06-12 | 2016-10-12 | 四川省农业科学院水产研究所 | Preservation method for benthonic animal sample |
-
2018
- 2018-02-06 CN CN201810120213.6A patent/CN108207929A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1887961A (en) * | 2006-07-28 | 2007-01-03 | 重庆师范大学 | Small biological sample preparing process with transparant water soluble resin |
| CN101636649A (en) * | 2007-02-27 | 2010-01-27 | 恰根有限公司 | Fixation of a biological material |
| CN103013437A (en) * | 2012-12-18 | 2013-04-03 | 中国人民解放军第三军医大学第一附属医院 | Interior label stationary liquid for biological specimen preserved through formalin, preparation method and application thereof |
| CN103583509A (en) * | 2013-11-27 | 2014-02-19 | 青岛海洋科技馆 | Making method of gel specimen |
| CN104585237A (en) * | 2015-02-11 | 2015-05-06 | 广东海洋大学 | Environmentally-friendly composite anti-mildew and antiseptic agent and application thereof |
| CN105994249A (en) * | 2016-06-12 | 2016-10-12 | 四川省农业科学院水产研究所 | Preservation method for benthonic animal sample |
Non-Patent Citations (3)
| Title |
|---|
| 唐春红著: "《天然防腐剂与抗氧化剂》", 31 May 2010 * |
| 姜昌富，黄庆华: "《食源性病原生物检测技术》", 30 April 2003 * |
| 郑清梅: "《生物资源与利用丛书 动物学野外实习指导》", 30 September 2017 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109497035A (en) * | 2018-11-19 | 2019-03-22 | 张昌盛 | Bone and bone derived tissues sample preservation protective agent and preparation method |
| CN110476949A (en) * | 2019-08-22 | 2019-11-22 | 云南省热带作物科学研究所 | A kind of Slide processing of Africangiant snail |
| CN110476949B (en) * | 2019-08-22 | 2021-09-14 | 云南省热带作物科学研究所 | Preparation method of specimen of African big snail |
| CN110663678A (en) * | 2019-10-16 | 2020-01-10 | 广西师范大学 | Method for manufacturing amphibious specimen |
| CN110692626A (en) * | 2019-11-21 | 2020-01-17 | 钦州市中医药研究所 | Dry plant specimen and preparation method thereof |
| CN113142183A (en) * | 2021-02-23 | 2021-07-23 | 台州学院 | System and method for manufacturing ecological specimens of verruca virginiana |
| CN113142183B (en) * | 2021-02-23 | 2022-06-17 | 台州学院 | Ecological sample making devices of verruca virginiana |
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