CN103583509A

CN103583509A - Making method of gel specimen

Info

Publication number: CN103583509A
Application number: CN201310611064.0A
Authority: CN
Inventors: 王海铭; 杨翠华; 王玮
Original assignee: QINGDAO MARINE SCIENCE AND TECHNOLOGY MUSEUM
Current assignee: QINGDAO MARINE SCIENCE AND TECHNOLOGY MUSEUM
Priority date: 2013-11-27
Filing date: 2013-11-27
Publication date: 2014-02-19
Anticipated expiration: 2033-11-27
Also published as: CN103583509B

Abstract

A making method of a gel specimen belongs to the technical field of specimen making. The method comprises a step that a processed specimen is put in a specimen preservation object, the specimen preservation material comprises an upper layer and a lower layer, the upper layer is a liquid formaldehyde layer, and components of the solid gel layer comprises a gel, sugar and water. The depth of the upper-layer formaldehyde liquid is 1-2cm. The components of the solid gel layer concretely comprise 1-3 parts of the gel, 5-8 parts of the sugar and 500-600 parts of water. The method utilizes the transparent and compact characteristic of the gel to make an original specimen, the specimen does not deform or fade, and deformation and fading of the specimen are difficult to overcome. The preservation of the gel specimen in a gel form only needs a small amount of a preservation liquid comprising formaldehyde, alcohol and water, so the consumption of harmful substances is reduced, and harms of volatilization to the environment in the preservation process are reduced. The above used materials are heated as a high temperature, a small amount of formaldehyde is used for sealing, and a container is sealed, so the specimen will not decay.

Description

A kind of preparation method of gel sample

Technical field

The invention belongs to preparation of specimen's technical field, be specifically related to a kind of preparation method of gel sample.

Background technology

Aquatic livestock has beautiful morphological feature, and particularly some rare abyssopelagic organisms, beautiful in colour, and form is graceful, has very high ornamental value after making sample.At present, sample is generally preserved with formaldehyde or alcohol, in order to make sample present graceful form in preserving liquid, conventionally use glass thread or cotton thread binding fixing, but no matter be that alcohol or formaldehyde are preserved sample as preserving liquid, after placing a period of time, sample has just lost original color, if seal bad, formaldehyde volatilization and cause air pollution, because binding is out of shape or it is obvious to bundle vestige, finally make sample lose ornamental value simultaneously.

Summary of the invention

The technical problem to be solved in the present invention is to provide a kind of preparation method of gel sample, to solve prior art excessive use formaldehyde or alcohol in zoological specimens manufacturing process, thus the technical problem that operating personnel and environment are worked the mischief.And this technology, not by any instrument, can guarantee that sample swims in container, thus the displaying sample of ecosystem.

The present invention is achieved through the following technical solutions:

A preparation method for gel sample, puts into sample preservation object by the sample of handling well, and described sample preservation object is divided two-layer up and down, and upper strata is that liquid formaldehyde Ceng， lower floor is solid gel layer, and the composition of described solid gel layer comprises gel, sugar and water.

Further, the degree of depth of described supernatant liquid formaldehyde layer is 1-2cm.

Further, gel 1-3 part, sugared 5-8 part, water 500-600 part in the composition of described solid gel layer.

Further, the preparation method of described gel sample is:

1) ready gel, sugar and water are poured in clean container 1, heated while stirring, until solution boiling;

2) to pouring the solution in container 1 described in 2/5～3/5 volume step 1) in clean container 2 into, be placed on and in incubator, keep temperature, the solution described in remaining 2/5～3/5 volume is stayed in container 1, is slow cooling to 60 ℃;

3) sample of handling well is put into the step 2 of 60 ℃) described container 1, with fixture fixed preparation position and sample form, side by side bubble removing;

4) container described in step 3) 1 is put into refrigerator, to solution solidifies;

5) after the solution solidifies described in step 4), take out fixture, the solution in incubator inner pressurd vessel 2 is slowly poured in the container 1 after solution solidifies, avoid producing bubble, continue to put into refrigerator to solidifying;

6) toward the formalin sealing that adds 1-2cm in the container 1 after step 5) solution solidifies, airtight container 1.

The described sample of handling well is for use 5%(percent by volume) more than formalin immersion 24h sample.

The present invention's beneficial effect compared with prior art:

The present invention utilizes transparent, the fine and close feature of gel, make the sample of ecosystem, and sample is indeformable, colour-fast, and this is that sample preservation is difficult to the difficulty overcoming always.

The present invention preserves with gel form, only needs a small amount of formaldehyde, alcohol etc. to preserve liquid, has reduced the harm because of volatilization, environment being caused in the usage amount of harmful substance and preservation process.Because the material using all passes through high-temperature heating, and there are a small amount of formaldehyde sealing and seal of vessel, so sample can be not corrupt.

Embodiment

Below by embodiment, content of the present invention is further explained, but protection scope of the present invention is not subject to any pro forma restriction of embodiment.

Embodiment 1

A preparation method for gel sample, material used comprises two beakers, 1g gel, 5g sugar, 500ml water, 10ml formalin, concrete steps are as follows:

1) beaker 1 is poured 1g gel, 5g sugar and 500ml water into, is heated with stirring to boiling;

2) to pouring the solution in beaker 1 described in 200ml step 1) in beaker 2 into, be placed in insulation in incubator, residue 300ml solution is stayed in beaker 1, slow cooling to 60 ℃;

3) sample of handling well is put into the container 1 of 60 ℃, with glass fiber fixed preparation position and sample form, side by side bubble removing;

5) after the solution solidifies described in step 4), take out the glass fiber that fixed preparation is used, the solution in the container in incubator 2 is slowly poured in the container 1 after solution solidifies, avoid producing bubble, continue to put into refrigerator to solidifying;

6) toward the formalin sealing that adds 10ml in the container 1 after step 5) solution solidifies, airtight container 1.

The described sample of handling well is for through 5%(percent by volume) formalin soaks the jellyfish sample of 24h.Complete form, the good Papua glass gall mother of state are placed in container little, still energy full extension, slowly add saturated MgSO ₄solution, makes jellyfish holonarcosis;

1), according to the volume of water in container, calculate the formalin volume add commercially available 37-40%, be mixed with 5%(percent by volume) formalin, formaldehyde: the volume ratio of water is 5:95;

2) by the jellyfish of holonarcosis in 5%(percent by volume) soak 24h in formalin, standby.

Embodiment 2

A preparation method for gel sample, material used comprises two beakers, 3g gel, 8g sugar, 600ml water, 20ml formalin, concrete steps are as follows:

1) beaker 1 is poured 3g gel, 8g sugar and 600ml water into, is heated with stirring to boiling;

2) to pouring the solution in beaker 1 described in 300ml step 1) in beaker 2 into, be placed in insulation in incubator, residue 300ml solution is stayed in beaker 1, slow cooling to 60 ℃;

6) toward the formalin sealing that adds 20ml in the container 1 after step 5) solution solidifies, airtight container 1.

The described sample of handling well is for through 5%(percent by volume) formalin soaks the goldfish sample of 24h.

1) complete form, the good goldfish of state are placed in the container of 950ml water;

2) slowly add the formalin 50ml of commercially available 37-40%, be mixed with 5% formalin, soak 24h, standby.

Embodiment 3

Same embodiment 1 and the good sample of the same step process of embodiment 2 are made by the method for prior art

1) complete form, the good Papua glass gall mother of state are placed in container little, still energy full extension, slowly add saturated MgSO ₄solution, makes jellyfish holonarcosis;

2) according to the volume of water in container, calculate the formalin volume that adds commercially available 37-40%, be mixed with 5% formalin, formaldehyde: the volume ratio of water is 5:95;

3) by the jellyfish of holonarcosis in 5%(percent by volume) soak 24h in formalin;

4) with glass fiber or cotton thread, the jellyfish in step 3) is bundled on sheet glass, puts into specimen bottle and preserve, specimen preserving liquid is 5%(percent by volume) formalin.

Embodiment 4

2) slowly add the formalin 50ml of commercially available 37-40%, be mixed with percent by volume and be 5% formalin, soak 24h;

3) with glass fiber or cotton thread by step 2) in goldfish be bundled on sheet glass, put into specimen bottle and preserve, specimen preserving liquid is the long-pending percentage of 5%() formalin.

The sample that above-described embodiment is made is after placing 1 year, and contrast is found:

Embodiment 1 mini-bus cloth nitrous jellyfish can keep original blueness, and ecosystem floats in specimen bottle Zhong， museum exhibition cupboard in good shape, is conducive to the lifestyle that visitor understands this jellyfish.But the Papua glass gall in embodiment 3 is female, and body colour becomes white, and binding vestige is obvious, and jellyfish has shrink phenomenon.

It is original orange that goldfish in embodiment 2 can keep, and flake is bright, beautiful design in specimen bottle, and true reappearance is state before death.But the goldfish in embodiment 3, it is very shallow that body colour becomes, and binding vestige is obvious, and unencapsulated specimen bottle gives out harmful substance formaldehyde.

Claims

1. the preparation method of a gel sample, it is characterized in that its step is for to put into sample preservation object by the sample of handling well, described sample preservation object is divided two-layer up and down, and upper strata is liquid formaldehyde layer, lower floor is solid gel layer, and the composition of described solid gel layer comprises gel, sugar and water.

2. the preparation method of a kind of gel sample according to claim 1, is characterized in that the degree of depth of described liquid formaldehyde layer is 1-2cm.

3. the preparation method of a kind of gel sample according to claim 1, is characterized in that gel 1-3 part, sugared 5-8 part, water 500-600 part in the composition of described lower floor's solid layer.

4. the preparation method of a kind of gel sample according to claim 3, is characterized in that the preparation method of described gel sample is:

2) to the solution in the container 1 of pouring in clean container 2 described in 2/5～3/5 volume step 1), be placed on and in incubator, keep temperature, solution is stayed in container 1 described in remaining 2/5～3/5 volume, is slow cooling to 60 ℃;

3) sample of handling well is put into the container 1 of 60 ℃, with fixture fixed preparation position and sample form, side by side bubble removing;

5) after the solution solidifies described in step 4), take out fixture, the solution in the container in incubator 2 is slowly poured in the container 1 after solution solidifies, avoid producing bubble, continue to put into refrigerator to solidifying;

6) toward the formalin sealing that adds 1-2cm in the container 1 after solution solidifies described in step 5), airtight container 1.

5. the preparation method of a kind of gel sample according to claim 4, the sample of handling well described in it is characterized in that is for use 5%(percent by volume) more than formalin solution immersion 24h sample.