CN103814890A - Method for making large helminth specimen - Google Patents
Method for making large helminth specimen Download PDFInfo
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- CN103814890A CN103814890A CN201410115727.4A CN201410115727A CN103814890A CN 103814890 A CN103814890 A CN 103814890A CN 201410115727 A CN201410115727 A CN 201410115727A CN 103814890 A CN103814890 A CN 103814890A
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- polypide
- specimen
- helminth
- epoxy resin
- anesthesia
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Abstract
The invention belongs to the field of medical human parasitology and provides a method for making a large helminth specimen. The method comprises the following steps: specimen selection and collection; anesthetizing and precooling; freeze drying; epoxy resin embedding. The large helminth specimen made by using the method is capable of keeping the integrity of the appearance and shape of a helminth body for a long term, convenient to carry, diversified in appearance, free of preservative and pollutant volatilization, strong in transparency and easy to observe so as to meet the application requirement of parasitology in teaching and research processes and obtain better using effect and efficiency.
Description
Technical field
The invention belongs to medical science Human Parasite Science field, relate to a kind of method of making Vermes Parasite larvae and adult gross specimen, can be used for making parasite gross specimen, especially can in Human Parasitology Teaching and scientific research, use.
Background technology
At present, the making of parasite gross specimen both at home and abroad is still used traditional preparation method, puts into 5% ~ 10% formalin solution immersion after polypide is processed, and is stored in transparent glass or lucite bottle.Yielding, the variable color of sample that the method is preserved; Preserve liquid toxic volatile; The heavy shortcomings such as observation that are difficult for of sample.
Summary of the invention
For the deficiencies in the prior art, the object of this invention is to provide a kind of can preserve for a long time polypide, portable, be easy to the preparation method of parasite gross specimen of observing.
The preparation method of worm gross specimen provided by the invention, follows these steps to carry out:
The selection of a, polypide sample and collection: select complete, the active polypide that moves of polypide, rinse well with 37 ℃ of warm salines;
B, anesthesia and precooling: it is 25% ~ 35% alcoholic solution 5 ~ 10 minutes that polypide is inserted to concentration, after polypide anesthesia, takes out and puts into-20 ℃ of refrigerator precoolings 30 minutes;
C, freeze drying: polypide is put into vacuum freeze drier, 18 ~ 32 hours;
D, epoxy resin embedding: polypide is put into suitable mould, bicomponent epoxy resin A, B component are mixed in proportion, slowly pour mould into, treat that it is completely curing.
The invention has the beneficial effects as follows, the gross specimen of making by the method can preserve for a long time polypide outward appearance and form integrality, be easy to carry, the variation of sample profile, do not use preservative contamination-free volatilization, transparency strong, be easy to observe, to meet the application demand in parasitology teaching and scientific research process, obtain better result of use and efficiency.
Embodiment:
Embodiment 1
Diplacanthus nanus adult gross specimen is made, and step is as follows:
The selection of a, adult and collection: select length about 5cm, the active complete polypide of motion, rinse well with 37 ℃ of warm salines;
B, anesthesia and precooling: it is 25% alcoholic solution 5 minutes that polypide is inserted to concentration, after polypide anesthesia, takes out and puts into-20 ℃ of refrigerator precoolings 30 minutes;
C, freeze drying: polypide is put into vacuum freeze drier, 20 hours;
D, epoxy resin embedding: polypide is put into the cuboid mould of 8cm × 3cm × 3cm, bicomponent epoxy resin A, B component are mixed in proportion, slowly pour mould into, treat that it is completely curing.
Embodiment 2
Fasciolopsis buski adult gross specimen is made, and step is as follows:
The selection of a, adult and collection: select length about 6cm, the active complete polypide of motion, rinse well with 37 ℃ of warm salines;
B, anesthesia and precooling: it is 30% alcoholic solution 7 minutes that polypide is inserted to concentration, after polypide anesthesia, takes out and puts into-20 ℃ of refrigerator precoolings 30 minutes;
C, freeze drying: polypide is put into vacuum freeze drier, 24 hours;
D, epoxy resin embedding: polypide is put into the cuboid mould of 10cm × 5cm × 3cm, bicomponent epoxy resin A, B component are mixed in proportion, slowly pour mould into, treat that it is completely curing.
Embodiment 3
Ascaris lumbricoides larva gross specimen is made, and step is as follows:
The selection of a, adult and collection: select length about 10cm, the active complete polypide of motion, rinse well with 37 ℃ of warm salines;
B, anesthesia and precooling: it is 30% alcoholic solution 10 minutes that polypide is inserted to concentration, after polypide anesthesia, takes out and puts into-20 ℃ of refrigerator precoolings 30 minutes;
C, freeze drying: polypide is put into vacuum freeze drier, 30 hours;
D, epoxy resin embedding: polypide is put into the cuboid mould of 15cm × 3cm × 3cm, bicomponent epoxy resin A, B component are mixed in proportion, slowly pour mould into, treat that it is completely curing.
Claims (1)
1. a method of making Vermes Parasite larvae and adult gross specimen, is characterized in that:
The selection of a, polypide sample and collection: select complete, the active polypide that moves of polypide, rinse well with 37 ℃ of warm salines;
B, anesthesia and precooling: it is 25% ~ 35% alcoholic solution 5 ~ 10 minutes that polypide is inserted to concentration, after polypide anesthesia, takes out and puts into-20 ℃ of refrigerator precoolings 30 minutes;
C, freeze drying: polypide is put into vacuum freeze drier, 18 ~ 32 hours;
D, epoxy resin embedding: polypide is put into suitable mould, bicomponent epoxy resin A, B component are mixed in proportion, slowly pour mould into, treat that it is completely curing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410115727.4A CN103814890A (en) | 2014-03-26 | 2014-03-26 | Method for making large helminth specimen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410115727.4A CN103814890A (en) | 2014-03-26 | 2014-03-26 | Method for making large helminth specimen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103814890A true CN103814890A (en) | 2014-05-28 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410115727.4A Pending CN103814890A (en) | 2014-03-26 | 2014-03-26 | Method for making large helminth specimen |
Country Status (1)
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| CN (1) | CN103814890A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104075922A (en) * | 2014-07-04 | 2014-10-01 | 中国水产科学研究院淡水渔业研究中心 | Epoxy resin embedding method for preparing parasite sample for scanning electron microscope |
| CN105010302A (en) * | 2015-07-08 | 2015-11-04 | 南宁千年工艺有限公司 | Method for preparing resin-embedded water-containing animal specimen |
| CN108990959A (en) * | 2018-07-05 | 2018-12-14 | 安徽省农业科学院水产研究所 | A kind of production method of swamp eel parasitism spiny-headed worm sample |
| CN111590901A (en) * | 2020-06-11 | 2020-08-28 | 中国海洋大学 | Preparation method of octopus holographic digital modeling and 3D printing model |
| CN113678817A (en) * | 2021-08-02 | 2021-11-23 | 云南农业大学 | Method for preparing specimen of larva of rhynchophorus ferrugineus |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101162190A (en) * | 2007-11-22 | 2008-04-16 | 重庆师范大学 | Method for embedding biological specimen with high water content |
| CN101180958A (en) * | 2007-12-18 | 2008-05-21 | 重庆师范大学 | Processing method before imbedding biological specimen |
-
2014
- 2014-03-26 CN CN201410115727.4A patent/CN103814890A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101162190A (en) * | 2007-11-22 | 2008-04-16 | 重庆师范大学 | Method for embedding biological specimen with high water content |
| CN101180958A (en) * | 2007-12-18 | 2008-05-21 | 重庆师范大学 | Processing method before imbedding biological specimen |
Non-Patent Citations (3)
| Title |
|---|
| 张富强等: "用真空冷冻干燥法制作的蛇标本", 《动物学杂志》 * |
| 张远志: "冷冻干燥技术的应用", 《真空》 * |
| 祁云: "用冷冻干燥法制作蛙肺干制标本", 《生物学通报》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104075922A (en) * | 2014-07-04 | 2014-10-01 | 中国水产科学研究院淡水渔业研究中心 | Epoxy resin embedding method for preparing parasite sample for scanning electron microscope |
| CN105010302A (en) * | 2015-07-08 | 2015-11-04 | 南宁千年工艺有限公司 | Method for preparing resin-embedded water-containing animal specimen |
| CN105010302B (en) * | 2015-07-08 | 2017-10-10 | 南宁千年工艺股份有限公司 | A kind of preparation method of the aqueous zoological specimens of resin embedding |
| CN108990959A (en) * | 2018-07-05 | 2018-12-14 | 安徽省农业科学院水产研究所 | A kind of production method of swamp eel parasitism spiny-headed worm sample |
| CN111590901A (en) * | 2020-06-11 | 2020-08-28 | 中国海洋大学 | Preparation method of octopus holographic digital modeling and 3D printing model |
| CN111590901B (en) * | 2020-06-11 | 2021-11-16 | 中国海洋大学 | Preparation method of octopus holographic digital modeling and 3D printing model |
| CN113678817A (en) * | 2021-08-02 | 2021-11-23 | 云南农业大学 | Method for preparing specimen of larva of rhynchophorus ferrugineus |
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Application publication date: 20140528 |