CN105010302A - Method for preparing resin-embedded water-containing animal specimen - Google Patents
Method for preparing resin-embedded water-containing animal specimen Download PDFInfo
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- CN105010302A CN105010302A CN201510396645.6A CN201510396645A CN105010302A CN 105010302 A CN105010302 A CN 105010302A CN 201510396645 A CN201510396645 A CN 201510396645A CN 105010302 A CN105010302 A CN 105010302A
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Abstract
The invention provides a method for preparing a resin-embedded water-containing animal specimen. The method comprises the following steps: carrying out antiseptic treatment on a water-containing animal specimen, so as to obtain a treated specimen; submerging the treated specimen in a protective resin solution for surface treatment, so as to obtain a specimen to be embedded, wherein the protective resin solution contains an ingredient A and an ingredient B, the ingredient A is polyester polyol with the viscosity of 250-600cps, the ingredient B is isocyanate with the viscosity of 150-500cps, and the weight ratio of the ingredient A to the ingredient B is (1-2): 1; and carrying out resin embedding on the specimen to be embedded. By adopting the technical scheme provided by the invention, the specimen embedded in resin is lifelike in form and color, and the surface of the specimen is free of quality problems such as hazing and the like; embedding resin is free of hazing and turbid phenomena; the transparency of the resin is high, and all details of the specimen can be clearly observed; and in long-time use, the resin does not become yellow, and the specimen is not decayed.
Description
Technical field
The present invention relates to a kind of preparation method of sample, particularly relate to the preparation method of the moisture zoological specimens of a kind of resin embedding.
Background technology
With the moisture sample of resin embedding, be encapsulated in by sample in the middle of solid transparent resin, isolated extraneous air, makes sample can keep original form and color for a long time, avoids the injury of toxic preservatives to human body simultaneously.
Existing technology adopts the moisture sample of resin embedding, usually the quality problems easily occurred are: the moisture in (1) sample is exuded in the middle of resin and causes transparent resin muddy, White Flocculus and various impurity can be produced in resin crystal, sample surface atomizing, affect the observation to sample; (2) sample is shrivelled, color and metamorphosis obvious; (3) long-time storage, sample rots; (4) resin turns to be yellow in time, becomes opaque.
Summary of the invention
For above technical problem, the invention provides the preparation method of the moisture zoological specimens of a kind of resin embedding, adopt sample crystal water white transparency prepared by the method, the color of sample and form and living specimen basically identical, do not haze or other milky floccules in moisture sample surface and crystal, and sample is placed for a long time and also do not had variable color, hazes, the phenomenons such as crystal does not obviously turn to be yellow, explosion occur.
To this, the technical solution adopted in the present invention is: the preparation method of the moisture zoological specimens of a kind of resin embedding, comprises the following steps:
Step S1: carry out preservative treatment to moisture sample, obtains the sample after processing;
Step S2: the sample after described process is immersed in protection resin liquid and carry out surface treatment, obtain treating specimen embedding;
Wherein, described protection resin liquid comprises component A and B component, and described component A is PEPA, and described B component is isocyanates; The weight ratio of described component A and B component is 1 ~ 2:1;
Further optimization, the viscosity of described PEPA is 250-600cps, and the viscosity of described isocyanates is 150-500cps.
Step S3: treat that specimen embedding carries out resin embedding to described.
As a further improvement on the present invention, the weight ratio of described component A and B component is 1 ~ 1.8:1; .
As a further improvement on the present invention; in step S2; described surface-treated method is: immersed by the sample after described process in protection resin liquid; vacuumize and carry out surface treatment; processing time is 1 ~ 10 minute, and vacuum is 0.02 ~ 0.1MPa, is then taken out by sample; allow the resin solidification more than at least 6 hours on its surface, obtain treating specimen embedding.Wherein, described vacuum more preferably 0.02 ~ 0.05MPa.
As a further improvement on the present invention, described embeding resin liquid comprises main glue and curing agent, and the component that described main glue comprises and percentage by weight thereof are: epoxy resin 80-90%, reactive diluent 0.1-10%, promoter 0.1-8%, levelling agent 0.1-1%, ultra-violet absorber 0.1-1%; At least one in described curing agent polyetheramine, fatty amine or acid anhydrides.
As a further improvement on the present invention, in step S3, to the described method treating that specimen embedding carries out resin embedding be: first embeding resin liquid is poured in mould, to treat that specimen embedding is put on the embeding resin liquid of mould, wherein, treat described in that the degree of depth of specimen embedding immersion embeding resin liquid is less than the embeding resin liquid degree of depth in a mold; After embeding resin solidification, continue to add described embeding resin liquid, make it cover sample, solidification obtains the moisture zoological specimens of resin embedding.
As a further improvement on the present invention, the epoxide equivalent of described epoxy resin is 170-280g/eq, and the viscosity at 25 DEG C is 5000-25000eps.
As a further improvement on the present invention, described reactive diluent is at least one in C12-C14 aliphatic glycidyl ether, butyl glycidyl ether, orthoresol glycidol ether or 1.4 butanediol diglycidyl ethers; Described promoter is at least one in nonyl phenol or 2 methylimidazoles.
As a further improvement on the present invention, the percentage by weight of described main glue and curing agent is 3 ~ 5:1.
As a further improvement on the present invention, in step S1, after first being killed by sample chloroform, arrange form, then sample is immersed in impregnating fluid and keep 2 ~ 6 days, take out and arrange sample.
During described arrangement form, be first fixed on trim panel by the animal killed and arrange, the first solution needing solution to dig is dug to be fixed on trim panel again and is carried out arrangement form.
Wherein, when arranging sample, if crinite, hair electricity consumption dries up, finishing form.
As a further improvement on the present invention, the component that comprises of described impregnating fluid and percentage by weight thereof are: formaldehyde 3-10%, phenol 1-3%, glycerine 3-5%, glacial acetic acid 1-5%, water are supplied.
Compared with prior art, beneficial effect of the present invention is:
The first, adopt technical scheme of the present invention, the specimen morphology color be embedded in resin is true to nature, and sample surface such as not to haze at the quality problems.
The second, embeding resin does not haze, turbid phenomenon; Resin transparent degree is high, clearly can observe each details of sample.
3rd, use for a long time, resin does not turn yellow, and sample is not corrupt.
Embodiment
Below preferably embodiment of the present invention is described in further detail.
Embodiment 1
A kind of preparation method of resin embedding toad ambroid sample comprises the following steps:
1, live body toad chloroform kills, and then on trim panel, is fixed into jump form, is of a size of: 7*6.5*2cm, and water snake epidermis penetrates intensive aperture with syringe needle.
2, impregnating fluid preparation: the component of impregnating fluid and percentage by weight thereof are: formaldehyde 7.5%, phenol 1%, glycerine 1.5%, glacial acetic acid 3% and running water 87%, mix.
3, orthopedic toad and trim panel are immersed in impregnating fluid together, soak 3 days, take out, dry surface moisture, preparation surface process.
4, the preparation of surface treatment liquid: surface treatment liquid comprises component A and B component, component A is polyester polyol component, and B component is isocyanate component, and the mass ratio of component A and B component is 1:1, surface treatment liquid is obtained after component A and B component being mixed, immersed in surface treatment liquid by toad sample, put into vacuum tank and start to vacuumize, vacuum reaches 0.08MPA, keep 1 minute, continue to be evacuated down to 0.04MPA, keep 1 minute, both can exit.
5, take out the toad after process, the process on drip-dry surface residual night on filter screen, be placed on Antisticking and solidify 6 hours, resin embedding operation can be entered.
6, embeding resin preparation: embeding resin comprises main glue and curing agent, component and the percentage by weight thereof of main glue formula are: standard type epoxy resin NPEL128 87%, butyl glycidyl ether 7.5%, nonyl phenol 5% and ultra-violet absorber uv-196 0.5%, mix above-mentioned substance; The component of hardener formula and percentage by weight thereof are: polyetheramine D230 75% and different fluorine that ketone diamines IPDA 25%, both mix.
7, be that 3:1 mixes final vacuum deaeration and obtains embeding resin glue by embeding resin according to main glue and the mass ratio of curing agent, mix final vacuum deaeration, glue is poured into about 5mm height in 7.5*7.5*2.8cm acrylic mould, the toad sample handled well is above put into mould, the bottom of toad is soaked in 5mm resin liquid, solidify 6 hours, sample position fixes, continue the mixing glue adding surface compositions, until covered sample, concordant with acrylic mould upstream edge, solidify 12 hours, just obtain toad specimen embedding.
According to the resin embedding toad ambroid sample that the method makes, the color of toad sample and form and living specimen basically identical, each organ clearly can observing sample is as nose, eyes and ear and skin color and secretory gland, embeding resin is transparent, sample surface does not have vaporific and muddy thing, does not have impurity and floccule in resin crystal.This specimen embedding puts into 50 DEG C of baking ovens 24 hours, and transferring in-18 DEG C of refrigerator-freezers 24 hours, three times repeatedly, sample does not have variable color, hazes, and the quality problems such as crystal does not turn to be yellow, explosion occur; This specimen embedding is placed on outdoor natural aging 1 month, and sample does not have variable color, hazes, and the quality problems such as crystal does not obviously turn to be yellow, explosion occur.
Embodiment 2
A kind of preparation method of resin embedding water snake ambroid sample comprises the following steps:
1, live body water snake chloroform kills, and then on trim panel, is fixed into form of creeping, and is of a size of: 14*5.5*1.5cm.
2, impregnating fluid preparation: the component of impregnating fluid and percentage by weight thereof are: formaldehyde 10%, phenol 3%, glycerine 5%, glacial acetic acid 1.5% and running water 80.5%, mix.
3, orthopedic water snake and trim panel are immersed in impregnating fluid together, soak 5 days, take out, dry surface moisture, preparation surface process.
4, the preparation of surface treatment liquid: surface treatment liquid comprises component A and B component, component A is polyester polyol component, and B component is isocyanate component, and the mass ratio of component A and B component is 1.5:1, surface treatment liquid is obtained after component A and B component being mixed, immersed in surface treatment liquid by water snake sample, put into vacuum tank and start to vacuumize, vacuum reaches 0.08MPA, keep 3 minutes, continue to be evacuated down to 0.01MPA, keep 3 minutes, both can exit.
5, take out the water snake after process, the process on drip-dry surface residual night on filter screen, be placed on Antisticking and solidify 6 hours, resin embedding operation can be entered.
6, embeding resin preparation: embeding resin comprises main glue and curing agent, component and the percentage by weight thereof of main glue formula are: standard type epoxy resin NPEL128 89.5%, C12-C14 aliphatic glycidyl ether AGE 9.5%, glyoxal ethyline 1% and ultra-violet absorber uv-196 0.5%, mix above-mentioned substance; The component of curing agent and percentage by weight thereof are: polyetheramine T403 60%, polyetheramine D400 30% and m-xylene diamine 10%, three mixes.
7, be that 4.5:1 mixes final vacuum deaeration and obtains embeding resin glue by embeding resin according to main glue and the mass ratio of curing agent, again embeding resin glue is poured into about 5mm height in 160*7.5*2.8cm acrylic mould, the water snake sample handled well is above put into mould, the bottom of water snake is soaked in 5mm resin liquid, solidify 6 hours, sample position fixes, continue the embeding resin glue adding surface compositions, until covered sample, concordant with acrylic mould upstream edge, solidify 12 hours, just obtain water snake specimen embedding.
According to the resin embedding water snake ambroid sample that the method makes, crystal water white transparency, the color of water snake sample and form are basically identical with living specimen, do not haze or other milky floccules in surperficial and crystal at moisture sample.This specimen embedding puts into 50 DEG C of baking ovens 24 hours, and transferring in-18 DEG C of refrigerator-freezers 24 hours, three times repeatedly, sample does not have variable color, hazes, and the quality problems such as crystal does not turn to be yellow, explosion occur; This specimen embedding is placed on outdoor natural aging 1 month, and sample does not have variable color, hazes, and outward appearance is not obviously turned to be yellow, and occurs without quality problems such as explosions.
Above content is in conjunction with concrete preferred embodiment further description made for the present invention, can not assert that specific embodiment of the invention is confined to these explanations.For general technical staff of the technical field of the invention, without departing from the inventive concept of the premise, some simple deduction or replace can also be made, all should be considered as belonging to protection scope of the present invention.
Claims (10)
1. a preparation method for the moisture zoological specimens of resin embedding, is characterized in that, comprise the following steps:
Step S1: carry out preservative treatment to moisture zoological specimens, obtains the sample after processing;
Step S2: the sample after described process is immersed in protection resin liquid and carry out surface treatment, obtain treating specimen embedding;
Wherein, described protection resin liquid comprises component A and B component, and described component A is PEPA, and described B component is isocyanates; The weight ratio of described component A and B component is 1 ~ 2:1;
Step S3: treat that specimen embedding carries out resin embedding to described.
2. the preparation method of the moisture zoological specimens of resin embedding according to claim 1, is characterized in that: the weight ratio of described component A and B component is 1 ~ 1.8:1.
3. the preparation method of the moisture zoological specimens of resin embedding according to claim 1; it is characterized in that: in step S2; described surface-treated method is: immersed by the sample after described process in protection resin liquid; vacuumize and carry out surface treatment; processing time is 1 ~ 10 minute, and vacuum is 0.02 ~ 0.1MPa, is then taken out by sample; allow the resin solidification more than at least 6 hours on its surface, obtain treating specimen embedding.
4. the preparation method of the moisture zoological specimens of the resin embedding according to claim 1 ~ 3 any one, it is characterized in that: described embeding resin liquid comprises main glue and curing agent, the component that described main glue comprises and percentage by weight thereof are: epoxy resin 80-90%, reactive diluent 0.1-10%, promoter 0.1-8%, levelling agent 0.1-1%, ultra-violet absorber 0.1-1%; At least one in described curing agent polyetheramine, fatty amine or acid anhydrides.
5. the preparation method of the moisture zoological specimens of resin embedding according to claim 4, it is characterized in that: in step S3, to the described method treating that specimen embedding carries out resin embedding be: first embeding resin liquid is poured in mould, to treat that specimen embedding is put on the embeding resin liquid of mould, wherein, treat described in that the degree of depth of specimen embedding immersion embeding resin liquid is less than the embeding resin liquid degree of depth in a mold; After embeding resin solidification, continue to add described embeding resin liquid, make it cover sample, solidification obtains the moisture zoological specimens of resin embedding.
6. the preparation method of the moisture zoological specimens of resin embedding according to claim 4, is characterized in that: the epoxide equivalent of described epoxy resin is 170-280g/eq, and the viscosity at 25 DEG C is 5000-25000eps.
7. the preparation method of the moisture zoological specimens of resin embedding according to claim 4, is characterized in that: described reactive diluent is at least one in C12-C14 aliphatic glycidyl ether, butyl glycidyl ether, orthoresol glycidol ether or 1.4 butanediol diglycidyl ethers; Described promoter is at least one in nonyl phenol or 2 methylimidazoles.
8. the preparation method of the moisture zoological specimens of resin embedding according to claim 4, is characterized in that: the percentage by weight of described main glue and curing agent is 3 ~ 5:1.
9. the preparation method of the moisture zoological specimens of the resin embedding according to claim 1 ~ 3 any one, is characterized in that: in step S1, after first being killed by sample chloroform, arranges form, is then immersed by sample in impregnating fluid and keep 2 ~ 6 days, take out and arrange sample.
10. the preparation method of the moisture zoological specimens of resin embedding according to claim 9, is characterized in that: the component that described impregnating fluid comprises and percentage by weight thereof are: formaldehyde 3-10%, phenol 1-3%, glycerine 3-5%, glacial acetic acid 1-5%, water are supplied.
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| CN106818703A (en) * | 2017-03-28 | 2017-06-13 | 青岛农业大学 | A kind of small fishes primary colors solid phase Slide processing |
| CN107063810A (en) * | 2016-12-09 | 2017-08-18 | 中国科学院海洋研究所 | The novel method of marine organism specimen is preserved using epoxy resin |
| CN107136051A (en) * | 2017-07-17 | 2017-09-08 | 广州越城丰茂农林科技有限公司 | A kind of preparation method of amber sample |
| CN107183002A (en) * | 2017-05-10 | 2017-09-22 | 广西柳州中嘉知识产权服务有限公司 | Butterfly specimen preparation method |
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| CN107063810A (en) * | 2016-12-09 | 2017-08-18 | 中国科学院海洋研究所 | The novel method of marine organism specimen is preserved using epoxy resin |
| CN106818703A (en) * | 2017-03-28 | 2017-06-13 | 青岛农业大学 | A kind of small fishes primary colors solid phase Slide processing |
| CN107183002A (en) * | 2017-05-10 | 2017-09-22 | 广西柳州中嘉知识产权服务有限公司 | Butterfly specimen preparation method |
| CN107136051A (en) * | 2017-07-17 | 2017-09-08 | 广州越城丰茂农林科技有限公司 | A kind of preparation method of amber sample |
| CN108244093A (en) * | 2018-04-10 | 2018-07-06 | 大连理工大学 | A kind of insect specimen color preserving agent, preparation method and color-retention method |
| CN108583136A (en) * | 2018-04-28 | 2018-09-28 | 丁思尧 | A kind of Hezhe's fish-skin craftwork and preparation method thereof |
| CN108877446A (en) * | 2018-07-09 | 2018-11-23 | 张家港市德仁科教仪器设备有限公司 | A kind of foot collagen position embedding method and model |
| CN109994032A (en) * | 2019-01-25 | 2019-07-09 | 胡岳 | A method of human body cast moulds are saved by amber investment |
| CN109859601A (en) * | 2019-04-04 | 2019-06-07 | 江苏农牧科技职业学院 | The method of the alginates gap filler production coloured internal organs disjunctor exsiccata of buffalo multisystem |
| CN110558308A (en) * | 2019-09-24 | 2019-12-13 | 河南中博生物塑化科技有限公司 | Moisture-containing soft-buried section specimen and manufacturing method thereof |
| CN111213632A (en) * | 2019-11-19 | 2020-06-02 | 长春中医药大学 | Method and equipment for making animal medicine epoxy resin specimen |
| CN111213633A (en) * | 2019-11-19 | 2020-06-02 | 长春中医药大学 | Method and equipment for preparing fresh animal medicine epoxy resin specimen |
| CN113142184A (en) * | 2021-04-08 | 2021-07-23 | 浙江海洋大学 | Method for manufacturing marine organism specimen for teaching |
| CN115836675A (en) * | 2022-11-28 | 2023-03-24 | 南宁千帆教学科技有限公司 | Method for manufacturing embedded specimen |
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