CN101162190A - Method for embedding biological specimen with high water content - Google Patents
Method for embedding biological specimen with high water content Download PDFInfo
- Publication number
- CN101162190A CN101162190A CN 200710093019 CN200710093019A CN101162190A CN 101162190 A CN101162190 A CN 101162190A CN 200710093019 CN200710093019 CN 200710093019 CN 200710093019 A CN200710093019 A CN 200710093019A CN 101162190 A CN101162190 A CN 101162190A
- Authority
- CN
- China
- Prior art keywords
- sample
- embedding
- polyglycol
- lauxite
- specimen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to an encapsulating method for a biological specimen. The technical proposal adopted is that: a biological specimen is fixed, sterilized, antisepticised and cleaned, polyoxyethylene (PEG six hundred) is used to plasticize the specimen, the plasticized specimen is placed in a porcelain dish which is provided with absorbent paper, is then put into an incubator of thirty degrees to forty degrees for twelve to twenty-four hours and are finally fully integrated with a prepared transparent Lauxite until the preparation of specimen is completed. The encapsulating method has the advantages of a good encapsulating effect, mould proofing, insect prevention and color retention, which is particularly applicable to encapsulating the biological specimen with more water content, such as Craspedacusta, earthworms, mollusks and small-sized fishes.
Description
Technical field
The present invention relates to the embedding method of biological sample, particularly to the embedding method of the more biological sample of water cut.
Background technology
Present existing technology mainly is confined to small insects and small-sized plant with transparent Lauxite embedding biological sample, for the many biological samples of cylinder water content, as without special processing, can not be used for embedding.
Summary of the invention
The present invention is directed to the deficiency of existing embedding techniques, the method for the many biological samples of a kind of embedding water cut is provided, concrete technical scheme is as follows:
A kind of embedding method to the more biological sample of water cut is characterized in that carrying out according to the following steps:
(1) biological sample of gathering is fixed, sterilization, anticorrosion, clean, plastify with polyglycol;
(2) take out with the sample after the polyglycol plasticizing and also put it in the porcelain dish that is placed with thieving paper, go to place after 12～24 hours in 30～40 ℃ of incubators standby;
(3) biological sample of getting after the plasticizing of the transparent Lauxite for preparing and polyglycol is integrated into this embedding of rower fully: add transparent Lauxite and make primer in plastic containers, treat to put into after it solidifies the sample of step (2) gained, and insert sample with insect needle or stainless steel wire and fix, slowly add transparent Lauxite up to sample is covered embedding fully, treat to add once more after it solidifies transparent Lauxite and make to bind glue, this moment rotatable insect needle and pin taken out; After treating the complete setting and hardening of Lauxite, take out products made thereby.
Step (2) gained sample is drawn with thieving paper before embedding again, to remove the unnecessary polyglycol in sample surface.
Preferably using molecular weight in the plasticizing process is 600 polyglycol.
The described plasticizing process of step (1) is Chinese invention patent 200510020434.9 disclosed plasticizing methods.
The present invention compared with the prior art, difference is sample to be plastified with polyglycol before the embedding, so not only can keep the original attitude of sample in good condition, also can and colloid between well merge, phenomenon can not occur peeling off, and colloid itself also can keep pellucidity.
The present invention can remedy the deficiency of existing embedding techniques, and the many biological samples of energy embedding water cut are as craspedacusta sowerbyi, earthworm, mollusc, small fishes etc.; And this method embedding effect is better, has advantages such as mildew-resistant, insect protected, guarantor's look.
Specific embodiment
The embedding of craspedacusta sowerbyi sample
(1) craspedacusta sowerbyi is fixed, sterilization, anticorrosion; Clean: the sample that will be dipped in the antiseptic takes out, and washes repeatedly with clear water antiseptic is removed; Plasticizing: working concentration gradient molecular weight from low to high be 600 polyglycol as plasticiser, sample is plastified, so that polyglycol is replaced out the moisture in the sample fully;
(2) sample that takes out after the polyglycol plasticizing also puts it in the porcelain dish that is placed with thieving paper, goes in 30～40 ℃ of incubators and places 12～24 hours, to finish the processing of sample before the embedding;
(3) biological sample of getting after the transparent Lauxite for preparing and the plasticizing merges fully: add transparent Lauxite and make primer, treat to put into after it solidifies the sample of step (2) gained, and insert sample with insect needle or stainless steel wire and fix, slowly add transparent Lauxite up to sample is covered embedding fully, add transparent Lauxite once more and make to bind glue, this moment rotatable insect needle or stainless steel wire and with its taking-up.Finish until technology.
Claims (2)
1. embedding method to the more biological sample of water cut is characterized in that carrying out according to the following steps:
(1) biological sample of gathering is fixed, sterilization, anticorrosion, clean, plastify with polyglycol;
(2) take out with the sample after the polyglycol plasticizing and also put it in the porcelain dish that is placed with thieving paper, go to place after 12～24 hours in 30～40 ℃ of incubators standby;
(3) biological sample of getting after the plasticizing of the transparent Lauxite for preparing and polyglycol is integrated into this embedding of rower fully: add transparent Lauxite and make primer in plastic containers, treat to put into after it solidifies the sample of step (2) gained, and insert sample with insect needle or stainless steel wire and carry out with fixed, slowly add transparent Lauxite up to sample is covered embedding fully, treat to add once more after it solidifies transparent Lauxite and make to bind glue, this moment rotatable insect needle and pin taken out; After treating the complete setting and hardening of Lauxite, take out products made thereby.
2. according to claim 1 to the embedding method of the more biological sample of water cut, it is characterized in that: the molecular weight of described polyglycol is 600.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200710093019 CN101162190A (en) | 2007-11-22 | 2007-11-22 | Method for embedding biological specimen with high water content |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200710093019 CN101162190A (en) | 2007-11-22 | 2007-11-22 | Method for embedding biological specimen with high water content |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101162190A true CN101162190A (en) | 2008-04-16 |
Family
ID=39297147
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200710093019 Pending CN101162190A (en) | 2007-11-22 | 2007-11-22 | Method for embedding biological specimen with high water content |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101162190A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103115809A (en) * | 2013-01-23 | 2013-05-22 | 浙江大学 | Transmission electron microscope processing method for insect antenna samples |
| CN103814890A (en) * | 2014-03-26 | 2014-05-28 | 青岛大学 | Method for making large helminth specimen |
| CN104996397A (en) * | 2015-07-03 | 2015-10-28 | 陕西省微生物研究所 | Macrofungi specimen preparation method |
| CN105010302A (en) * | 2015-07-08 | 2015-11-04 | 南宁千年工艺有限公司 | Method for preparing resin-embedded water-containing animal specimen |
| CN104392653B (en) * | 2014-12-19 | 2016-08-17 | 陈军凤 | The method for shaping of piscine organism sample |
| CN107183002A (en) * | 2017-05-10 | 2017-09-22 | 广西柳州中嘉知识产权服务有限公司 | Butterfly specimen preparation method |
| CN108871899A (en) * | 2018-07-12 | 2018-11-23 | 泰山医学院 | Microsection manufacture method for histocyte dyeing |
-
2007
- 2007-11-22 CN CN 200710093019 patent/CN101162190A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103115809A (en) * | 2013-01-23 | 2013-05-22 | 浙江大学 | Transmission electron microscope processing method for insect antenna samples |
| CN103115809B (en) * | 2013-01-23 | 2016-04-27 | 浙江大学 | A kind of sample for use in transmitted electron microscope disposal route of insect feeler |
| CN103814890A (en) * | 2014-03-26 | 2014-05-28 | 青岛大学 | Method for making large helminth specimen |
| CN104392653B (en) * | 2014-12-19 | 2016-08-17 | 陈军凤 | The method for shaping of piscine organism sample |
| CN104996397A (en) * | 2015-07-03 | 2015-10-28 | 陕西省微生物研究所 | Macrofungi specimen preparation method |
| CN105010302A (en) * | 2015-07-08 | 2015-11-04 | 南宁千年工艺有限公司 | Method for preparing resin-embedded water-containing animal specimen |
| CN107183002A (en) * | 2017-05-10 | 2017-09-22 | 广西柳州中嘉知识产权服务有限公司 | Butterfly specimen preparation method |
| CN108871899A (en) * | 2018-07-12 | 2018-11-23 | 泰山医学院 | Microsection manufacture method for histocyte dyeing |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101162190A (en) | Method for embedding biological specimen with high water content | |
| CN101180958B (en) | Processing method before imbedding biological specimen | |
| CN101520961B (en) | Method for manufacturing crocodile specimen | |
| CN103548817B (en) | Preparation method of plant plasticized specimen | |
| Jonker et al. | Unusual adhesive production system in the barnacle Lepas anatifera: an ultrastructural and histochemical investigation | |
| CN100567941C (en) | Technique for fabricating label type biologic slice | |
| CN103392694B (en) | Method for preparing tropical plant preserving humid preparation | |
| CN106893329B (en) | A method of using fish processing fent leather for natural collagen film | |
| CN107063810A (en) | The novel method of marine organism specimen is preserved using epoxy resin | |
| Jaeckle et al. | Experimental manipulations of the organic compositions of seawater: implications for studies of energy budgets in marine invertebrate larvae | |
| CN104083299A (en) | Preparation method and product of deep sea fish skin collagen polypeptide peeling mask | |
| CN102747069A (en) | Non-invasive extraction method of Haliotis discus hannai ino genome DNA | |
| CN108207929A (en) | A kind of store method of zoobenthos sample | |
| Mizuta et al. | Partial characterization of collagen in mantle and adductor of pearl oyster (Pinctada fucata) | |
| CN113439741A (en) | Preparation method of primary color soaked specimen of snake mushroom | |
| CN110934130A (en) | Method for manufacturing ornamental fish embedding specimen | |
| CN106337074A (en) | Cirrhinus molitorella bone collagen extracting method | |
| DE69018813D1 (en) | METHOD FOR PRODUCING A TRANSGENIC; NOT HUMAN ANIMALS BY INTRODUCING EXOGENIC DNA IN ANIMAL CELLS; THE SOMATIC AND KEIMBAHN ORIGIN. | |
| CN108112520A (en) | A kind of method that stone living is made of oyster shell | |
| CN108409855A (en) | A kind of preparation method using fish scale collagen | |
| WO2008097884A3 (en) | Processing skin from living donors | |
| Chaparro et al. | Feeding behaviour during brooding in the oyster Ostrea chilensis: gut retention time in adults and larvae and potential use of larval faeces by adults | |
| CN112120113A (en) | Abalone juice extracting and processing system and processing method thereof | |
| CN104940025A (en) | Abalone shell water-soluble matrix and preparation method and application thereof | |
| CN1919624A (en) | Technique for manufacturing decorative picture using aquatic animal limb |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Open date: 20080416 |