CN114568421B - Plasticized specimen for exhibiting anatomical features of fascia fiber architecture and anatomical method - Google Patents
Plasticized specimen for exhibiting anatomical features of fascia fiber architecture and anatomical method Download PDFInfo
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- CN114568421B CN114568421B CN202210317644.8A CN202210317644A CN114568421B CN 114568421 B CN114568421 B CN 114568421B CN 202210317644 A CN202210317644 A CN 202210317644A CN 114568421 B CN114568421 B CN 114568421B
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- 210000003195 fascia Anatomy 0.000 title claims abstract description 54
- 239000000835 fiber Substances 0.000 title claims abstract description 19
- 238000000034 method Methods 0.000 title claims abstract description 15
- 230000001747 exhibiting effect Effects 0.000 title claims description 4
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims abstract description 50
- 238000005238 degreasing Methods 0.000 claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 15
- 239000011521 glass Substances 0.000 claims abstract description 13
- 239000011259 mixed solution Substances 0.000 claims abstract description 13
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims abstract description 12
- 241001465754 Metazoa Species 0.000 claims abstract description 12
- 239000007788 liquid Substances 0.000 claims abstract description 10
- 239000000243 solution Substances 0.000 claims abstract description 7
- 238000004061 bleaching Methods 0.000 claims abstract description 6
- 230000018044 dehydration Effects 0.000 claims abstract description 6
- 238000006297 dehydration reaction Methods 0.000 claims abstract description 6
- 238000004519 manufacturing process Methods 0.000 claims abstract description 6
- 238000002791 soaking Methods 0.000 claims abstract description 6
- 238000005520 cutting process Methods 0.000 claims abstract description 5
- 238000009966 trimming Methods 0.000 claims abstract description 5
- 238000009740 moulding (composite fabrication) Methods 0.000 claims abstract description 4
- 238000005070 sampling Methods 0.000 claims abstract description 4
- 238000011010 flushing procedure Methods 0.000 claims abstract description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 2
- 238000004321 preservation Methods 0.000 claims description 2
- 238000010008 shearing Methods 0.000 claims description 2
- 238000003892 spreading Methods 0.000 claims description 2
- 230000002421 anti-septic effect Effects 0.000 abstract 1
- 238000010276 construction Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 210000000115 thoracic cavity Anatomy 0.000 description 3
- 238000002224 dissection Methods 0.000 description 2
- 210000000629 knee joint Anatomy 0.000 description 2
- 210000003041 ligament Anatomy 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000000038 chest Anatomy 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 210000001624 hip Anatomy 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 229920001225 polyester resin Polymers 0.000 description 1
- 239000004645 polyester resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 238000007447 staining method Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
-
- G—PHYSICS
- G09—EDUCATION; CRYPTOGRAPHY; DISPLAY; ADVERTISING; SEALS
- G09B—EDUCATIONAL OR DEMONSTRATION APPLIANCES; APPLIANCES FOR TEACHING, OR COMMUNICATING WITH, THE BLIND, DEAF OR MUTE; MODELS; PLANETARIA; GLOBES; MAPS; DIAGRAMS
- G09B23/00—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes
- G09B23/28—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes for medicine
- G09B23/30—Anatomical models
-
- G—PHYSICS
- G09—EDUCATION; CRYPTOGRAPHY; DISPLAY; ADVERTISING; SEALS
- G09B—EDUCATIONAL OR DEMONSTRATION APPLIANCES; APPLIANCES FOR TEACHING, OR COMMUNICATING WITH, THE BLIND, DEAF OR MUTE; MODELS; PLANETARIA; GLOBES; MAPS; DIAGRAMS
- G09B23/00—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes
- G09B23/28—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes for medicine
- G09B23/30—Anatomical models
- G09B23/306—Anatomical models comprising real biological tissue
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- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pure & Applied Mathematics (AREA)
- Mathematical Physics (AREA)
- Chemical & Material Sciences (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Theoretical Computer Science (AREA)
- Algebra (AREA)
- Computational Mathematics (AREA)
- Educational Technology (AREA)
- Mathematical Analysis (AREA)
- Mathematical Optimization (AREA)
- Educational Administration (AREA)
- Business, Economics & Management (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a plasticizing specimen and an anatomical method for displaying anatomical features of fascia fibers, the method comprises S1, performing anatomical sampling of fascia in a target area after antiseptic fixation of a part separated from a human body or a large animal to obtain the fascia specimen; s2, bleaching the fascia specimen with hydrogen peroxide, and then soaking and flushing with small flowing water; s3, expanding the specimen, and flattening the fascia specimen; fixing a specimen by using a splint grid, and respectively soaking the specimen in acetone liquid at two different temperatures for dehydration; s4, degreasing in acetone liquid with different concentrations at normal temperature for a plurality of times; s5, manufacturing a glass clamping piece, and adding the glass clamping piece into the P45 mixed solution for full embedding; completely impregnating the P45 mixed solution under the low-temperature negative pressure state, and placing the impregnated solution into a water bath box for hardening; s6, taking out the transparent hardened fascia specimen, and cutting, trimming and forming. The invention realizes the comprehensive display of the anatomical features of the fibrous structures of fascia at a certain part of a human body or a large animal on a macroscopic level.
Description
Technical Field
The invention relates to the technical field of biology, in particular to a plasticized specimen for displaying anatomical features of fascia fiber construction and an anatomical method.
Background
The existing anatomical specimens and anatomical methods have limitations in the display of the fascial fiber architecture. The most common formalin-fixed general anatomic specimens are capable of observing the overall morphology of the fascia but not the fibrous structure inside the fascia. In contrast, microscopic histological sections and staining methods allow the display of a small range of fascia fiber structures by serial sections, but do not allow the display of a large range of fascia fiber structures anatomical features. In addition, formalin-fixed tomographic specimens can display only the positional information of fascia on the specimen cross section. The newly occurring tomograms plasticize the specimen and only reveal the anatomical features of the fascia fibers on the specimen cross-section. At present, no specimen and technology exist, and anatomical features of fibrous structures of fascia at a certain part of a large animal are comprehensively displayed on a macroscopic level.
Disclosure of Invention
In view of the above, the present invention has an object to provide a plasticized specimen and an anatomical method for exhibiting anatomical features of fascia fiber structures, which are capable of providing a specimen preparation method and a scientific research method for widely displaying anatomical features of fascia fiber structures of a part of a human body or a large animal.
The adopted technical scheme is as follows:
the invention discloses an anatomical method for displaying anatomical features of fascia fiber construction, which comprises the following steps:
s1, after the part separated from a human body or a large animal is preserved and fixed, dissecting and sampling fascia of a target area to obtain a fascia specimen;
s2, bleaching the fascia specimen with hydrogen peroxide, and then soaking and flushing with small flowing water;
s3, spreading the specimen, shearing the specimen through a non-main observation area, and flattening the fascia specimen; fixation using splinting grid
Respectively immersing the sample in two acetone liquids at different temperatures for dehydration;
s4, degreasing in acetone liquid with different concentrations at normal temperature for a plurality of times;
s5, manufacturing a glass clamping piece, and adding the P45 mixed solution; placing the dehydrated and defatted fascia specimen into the P45 mixed solution and fully embedding; then the P45 mixed solution is completely impregnated under the low-temperature negative pressure state, and then the mixed solution is put into a water bath box for hardening;
s6, placing the evacuated glass clamping piece into a water bath box for hardening, then disassembling the glass clamping piece, taking out the transparent hardened fascia specimen, cutting and trimming for forming, and finishing the specimen manufacture.
Further, in S1, the part separated from the human body or the large animal is fixed by formalin solution preservation.
Further, in S2, the fascia specimen is bleached with 5% hydrogen peroxide, observed once per hour, to prevent over-bleaching, and after 24 hours, soaked with small running water for 1 day.
Further, in S3, fixing the specimen by using a splint grid, and respectively soaking in acetone liquid at-25 ℃ and-15 ℃ for dehydration; dehydrating for the first time, 90% acetone at-25deg.C for 3 days; the second dehydration, 95% acetone at-15 ℃ for 4 days.
Further, in S4, degreasing in acetone liquid with different concentrations at normal temperature for 3 times; degreasing for 5 days by using 96% acetone for the first time; degreasing for the second time, namely degreasing for 5 days by using 98% acetone; degreasing for 7 days by using 99.9% acetone.
Further, in S5, the P45 mixed solution was completely impregnated at a temperature of 0℃and a pressure of-0.1 MPa for 12 hours, and then placed in a water bath to be cured.
And further, in S6, placing the evacuated glass clamping piece into a water bath box at the temperature of 32-35 ℃ for hardening, disassembling the glass clamping piece after 5 days, taking out a transparent hardened fascia specimen, and cutting, trimming and forming.
The invention discloses a plasticized specimen for displaying anatomical features of fascia fiber construction, which is prepared by adopting the above-mentioned anatomical method to finish specimen preparation.
The invention has the beneficial effects that:
the plasticizing specimen and the dissection method for displaying the anatomical features of the fascia fiber structures realize the purpose of displaying the anatomical features of the fibrous structures of the fascia of a certain part of a human body or a large animal more comprehensively on a macroscopic level, and can solve the bottleneck problem of long-term puzzled fibrous structure building researches of fascia structures, including the intra-fascia
Spatial construction features, start and stop or attachment sites, running direction, etc. of the fibers.
The invention has wide application value in biomedical teaching, life science popularization, biomedical scientific research, clinical and sports rehabilitation training and other aspects, and has extremely high potential social and economic benefits.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings referred to in the description of the embodiments will be briefly described below.
Fig. 1 is a view of a sample of the iliotibial band fiber structure around the knee joint of example 1.
Fig. 2 is a diagram of a specimen of the periknee ligament and fascia fiber construction of example 2.
FIG. 3 is a diagram showing a sample of the fibrous structure of the middle layer of the thoracic, lumbar and fascia in example 3.
FIG. 4 is a diagram showing a sample of the structure of the superficial fibers of the thoracic and lumbar fascia in example 4.
Wherein the scale bar below in figures 1-4 is in mm.
Detailed Description
The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is apparent that the described embodiments are only preferred embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
An anatomical method for displaying anatomical features of fascia fiber architecture of the present embodiment includes the steps of:
1. dissecting
And (3) performing formalin solution anti-corrosion fixation on the iliotibial band around the knee joint of the separated human body, and then performing dissection sampling of fascia and related structures of the target area to obtain a fascia specimen.
Bleaching
Fascia specimens were bleached with 5% hydrogen peroxide, observed once per hour, prevented from overcoating, and rinsed with small running water for 1 day after about 24 hours.
Dewatering
The specimen is unfolded and can be sheared through a non-primary observation area to flatten the fascia specimen. The specimen was fixed using a splint grid and dehydrated by immersing in acetone liquid at-25℃and-15℃respectively. Dehydrating for the first time, 90% acetone at-25deg.C for 3 days. The second dehydration, 95% acetone at-15 ℃ for 4 days.
Degreasing
Degreasing in acetone liquid with different concentrations at normal temperature for 3 times. Degreasing for the first time, 96% acetone, and 5 days. Degreasing for the second time, 98% acetone, and 5 days. Degreasing for the third time, 99.9% acetone and 7 days. Then P45 embedding is carried out.
Embedding
And (5) preparing a glass clamping piece, and adding the P45 mixed solution. And placing the dehydrated and defatted fascia specimen into the P45 mixed solution and fully embedding. The P45 mixture is then fully impregnated at a low temperature (e.g., 0deg.C) and negative pressure (e.g., -0.1 megapascals) for about 12 hours and then placed in a water bath to harden. Wherein the P45 mixed solution is the existing Hoffen P45 resin solution (Hoffen polyester P45). The P45 fault plasticizing technology belongs to the technology of polyester resin in biological plasticizing.
6. Hardening, namely placing the evacuated glass clamping piece into a 32 ℃ water bath box for hardening, disassembling the glass clamping piece after 5 days, taking out a transparent hardened fascia specimen, cutting, trimming and forming, and finishing the manufacture of a plasticized specimen.
The plasticized specimen sample is prepared as shown in fig. 1.
Example 2
Referring to example 1, unlike example 1, the part of the present example from which the human body or the large animal has been detached is the periknee ligament and fascia.
The plasticized specimen sample produced is shown in FIG. 2.
Example 3
Referring to example 1, unlike example 1, the part of the present example from which the human body or the large animal has been detached is the chest, waist and fascia middle layer.
The plasticized specimen sample produced is shown in FIG. 3.
Example 4
Referring to example 1, unlike example 1, the part of the present example from which the human body or the large animal has been separated is the shallow layer of the thoracic and lumbar fascia.
The plasticized specimen sample produced is shown in fig. 4.
The above list of detailed descriptions is only specific to practical embodiments of the present invention, and they are not intended to limit the scope of the present invention, and all equivalent embodiments or modifications that do not depart from the spirit of the present invention should be included in the scope of the present invention.
Claims (3)
1. An anatomical method of displaying anatomical features of a fascia fibrous structure, comprising the steps of: s1, after the part separated from a human body or a large animal is preserved and fixed, dissecting and sampling fascia of a target area to obtain a fascia specimen;
s2, bleaching the fascia specimen with hydrogen peroxide, and then soaking and flushing with small flowing water;
s3, spreading the specimen, shearing the specimen through a non-main observation area, and flattening the fascia specimen; fixing the specimen by using a splint grid, and respectively soaking in acetone liquid at-25 ℃ and-15 ℃ for dehydration; dehydrating for the first time, 90% acetone at-25deg.C for 3 days; dehydrating for the second time, and carrying out 4 days on 95% acetone at the temperature of minus 15 ℃;
s4, degreasing in acetone liquid with different concentrations at normal temperature for 3 times; degreasing for 5 days by using 96% acetone for the first time; degreasing for the second time, namely degreasing for 5 days by using 98% acetone; degreasing for the third time, namely degreasing for 7 days by using 99.9% acetone;
s5, manufacturing a glass clamping piece, and adding the P45 mixed solution; placing the dehydrated and defatted fascia specimen into the P45 mixed solution and fully embedding; then, under the condition that the temperature is 0 ℃ and the pressure is-0.1 megapascal, the P45 mixed solution is fully impregnated for 12 hours, and then the mixture is put into a water bath box for hardening;
s6, placing the evacuated glass clamping piece into a water bath box at 32-35 ℃ for hardening, removing the glass clamping piece after 5 days, taking out a transparent hardened fascia specimen, cutting, trimming and forming to finish specimen manufacturing;
in the step S2, the fascia specimen is bleached by 5% hydrogen peroxide, and is observed once per hour to prevent over bleaching, and is soaked and washed by small flowing water for 1 day after 24 hours.
2. The method according to claim 1, wherein in S1, the part separated from the human body or the large animal is fixed by formalin solution preservation.
3. A plasticized specimen exhibiting anatomical features of fascia fiber architecture prepared by the anatomical method of claim 1 or 2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210317644.8A CN114568421B (en) | 2022-03-29 | 2022-03-29 | Plasticized specimen for exhibiting anatomical features of fascia fiber architecture and anatomical method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210317644.8A CN114568421B (en) | 2022-03-29 | 2022-03-29 | Plasticized specimen for exhibiting anatomical features of fascia fiber architecture and anatomical method |
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| Publication Number | Publication Date |
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| CN114568421A CN114568421A (en) | 2022-06-03 |
| CN114568421B true CN114568421B (en) | 2023-12-05 |
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| CN202210317644.8A Active CN114568421B (en) | 2022-03-29 | 2022-03-29 | Plasticized specimen for exhibiting anatomical features of fascia fiber architecture and anatomical method |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102629430A (en) * | 2012-03-28 | 2012-08-08 | 上海中医药大学 | Manufacturing and identification method of meridian acupoint level anatomy teaching and research display specimen |
| CN111557299A (en) * | 2020-04-15 | 2020-08-21 | 昆山周庄鸿峰生命奥秘博物馆有限公司 | Specimen preparation method |
-
2022
- 2022-03-29 CN CN202210317644.8A patent/CN114568421B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102629430A (en) * | 2012-03-28 | 2012-08-08 | 上海中医药大学 | Manufacturing and identification method of meridian acupoint level anatomy teaching and research display specimen |
| CN111557299A (en) * | 2020-04-15 | 2020-08-21 | 昆山周庄鸿峰生命奥秘博物馆有限公司 | Specimen preparation method |
Non-Patent Citations (4)
| Title |
|---|
| Hoffen P45生物塑化技术在断层标本制作中的应用;白剑等;《中国临床解剖学杂志》;20100125(第01期);第107-108页 * |
| 人类项韧带的精细解剖结构;蒋爱民等;《中国组织工程研究与临床康复》;20070311(第10期);第1887-1891页 * |
| 塑化标本在人体解剖学教学中的应用;张德兴等;《解剖学研究》;19991231(第02期);第135-136页 * |
| 蒋爱民等.人类项韧带的精细解剖结构.《中国组织工程研究与临床康复》.2007,(第10期), * |
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