CN110558308A - Moisture-containing soft-buried section specimen and manufacturing method thereof - Google Patents
Moisture-containing soft-buried section specimen and manufacturing method thereof Download PDFInfo
- Publication number
- CN110558308A CN110558308A CN201910905312.XA CN201910905312A CN110558308A CN 110558308 A CN110558308 A CN 110558308A CN 201910905312 A CN201910905312 A CN 201910905312A CN 110558308 A CN110558308 A CN 110558308A
- Authority
- CN
- China
- Prior art keywords
- specimen
- moisture
- soft
- section specimen
- resin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
-
- G—PHYSICS
- G09—EDUCATION; CRYPTOGRAPHY; DISPLAY; ADVERTISING; SEALS
- G09B—EDUCATIONAL OR DEMONSTRATION APPLIANCES; APPLIANCES FOR TEACHING, OR COMMUNICATING WITH, THE BLIND, DEAF OR MUTE; MODELS; PLANETARIA; GLOBES; MAPS; DIAGRAMS
- G09B23/00—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes
- G09B23/36—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes for zoology
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Mathematical Optimization (AREA)
- Animal Behavior & Ethology (AREA)
- Mathematical Analysis (AREA)
- Algebra (AREA)
- Mathematical Physics (AREA)
- Pure & Applied Mathematics (AREA)
- Business, Economics & Management (AREA)
- Computational Mathematics (AREA)
- Educational Administration (AREA)
- Educational Technology (AREA)
- Theoretical Computer Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention relates to a moisture-containing soft-covered section specimen and a manufacturing method thereof, belonging to the technical field of biological resin covered sections. The method for manufacturing the moisture-containing soft-covered section specimen comprises the following steps: freezing and sawing a biological specimen into a sheet-shaped section specimen, soaking the section specimen in a water treatment agent for water treatment, and obtaining a pretreated section specimen after soaking; placing the pretreated section specimen in a mold, and pouring a soft embedding agent into the mold at a non-initiation temperature, wherein the soft embedding agent has flexibility after being cured; and (5) curing at the initiation temperature after the completion of the pouring, thus obtaining the product. Through soaking the section sample in moisture, can pin the moisture in the section sample completely, moisture can not come out again and influence the embedding. And the water is locked in the section specimen, so that the original state of the biological tissue can be kept, and the state of the biological tissue can be truly reproduced.
Description
Technical Field
The invention belongs to the technical field of biological resin embedded sections, and particularly relates to a moisture-containing soft-embedded section specimen and a manufacturing method thereof.
Background
The embedded specimen technology is applied to the history of nearly 30 years in the field of biological anatomical landmarks, and the biological plasticized specimen produced by utilizing the resin technology has the advantages of no toxicity, no odor, long-term preservation, convenient use, long-term preservation in the air and the like.
when the existing biological specimen is embedded, the biological specimen is generally embedded by hard resin, namely, the resin has higher hardness and no elasticity after being cured. There are also techniques for soft embedding. The soft embedded specimen is not easy to crack, has long preservation time and is clear, and can be bent to a plurality of more and directions. The invention patent with application publication number CN109523873A discloses a soft-covered section specimen and a manufacturing method thereof, wherein the manufacturing method comprises the following steps: pretreatment of biological tissues: freezing and sawing the biological specimen into a sheet-shaped section specimen, and dehydrating and degreasing the section specimen to obtain a pretreated section mark; placing the pretreated section specimen in a mold, and pouring a soft embedding medium into the mold at a non-initiation temperature, wherein the embedding medium is an embedding medium with softness after being cured; and (5) curing at the initiation temperature after the completion of the pouring, thus obtaining the product. When the soft embedding section specimen is manufactured, the biological specimen is frozen and sawed into the sheet-shaped section specimen, the section specimen is dehydrated and degreased, and after moisture and lipid of the specimen are removed, the original biological tissue shape is greatly changed and has a certain difference with the real biological tissue, so that the presented biological tissue is not in the original state of the biological tissue. In addition, the dehydration and degreasing in the preparation method adopt acetone treatment, so that the preparation method has the advantages of long time, long preparation period and high cost.
Disclosure of Invention
The first purpose of the invention is to provide a moisture-containing soft-buried fracture surface specimen which has good flexibility after being cured, is not easy to crack and deform, is convenient to store and use and can truly reproduce the form of the original biological tissue.
The second purpose of the invention is to provide a method for manufacturing the soft-covered fracture surface specimen.
In order to achieve the above purpose, the invention adopts the technical scheme that: the method for manufacturing the moisture-containing soft-covered section specimen comprises the following steps:
1) Pretreatment of biological tissues: freezing and sawing a biological specimen into a sheet-shaped section specimen, soaking the section specimen in a water treatment agent for water treatment, and obtaining a pretreated section specimen after soaking;
2) Placing the pretreated section specimen in the step 1) in a mould, and pouring a soft embedding agent into the mould at a non-initiation temperature, wherein the soft embedding agent has flexibility after being cured; and (5) curing at the initiation temperature after the completion of the pouring, thus obtaining the product.
Further, the water treatment agent in the step 1) comprises resin and an initiator, and the mass ratio of the resin to the initiator is 100: 0.2-100: 0.8.
Further, the resin is one or more of waterborne polyurethane acrylate, waterborne epoxy acrylate and waterborne polyester acrylate; the initiator is one or more of 2-methyl-1- [4- (methylthio) phenyl ] -2- (4-morpholinyl) -1-acetone, 2-methyl-1- (4-methylthiophenyl) -2-morpholinyl-1-acetone and 2-methyl-1- (4-methylthiophenyl) -2-morpholine-1-acetone IHT-PI 907).
Further, the water treatment agent also contains an anti-yellowing agent, and the mass ratio of the resin to the anti-yellowing agent is 100: 0.1-100: 0.5.
Further, the anti-yellowing agent is an epoxy resin anti-yellowing agent V76-PUv or an ultraviolet absorber UV-196.
Further, during water treatment, the section specimen is firstly placed in resin to be soaked for 5-10 min, then the section specimen is taken out to be naturally cured, then the section specimen is placed in resin to be soaked for 5-10 min, and the temperature of soaking and curing for two times is controlled to be 20-30 ℃.
Further, the soft embedding medium comprises the following components in parts by weight: 100 parts of UV curing resin with flexibility after UV curing and 0.2-0.8 part of photoinitiator, wherein the hardness of the UV curing resin after curing is Shore A60-A90.
Further, the UV curing resin is one or more of waterborne polyurethane acrylate, waterborne epoxy acrylate, waterborne polyester acrylate, polyurethane, acrylate and epoxy resin, the polyurethane is polyurethane acrylate, and the acrylate is one or more of polyester acrylate or polyether acrylate; the photoinitiator is one or more of 2-methyl-1- [4- (methylthio) phenyl ] -2- (4-morpholinyl) -1-acetone, 2-methyl-1- (4-methylthiophenyl) -2-morpholinyl-1-acetone and 2-methyl-1- (4-methylthiophenyl) -2-morpholine-1-acetone IHT-PI 907).
Further, the soft embedding medium further comprises an anti-yellowing agent in an amount of 0.2-0.6 parts by mass, and the anti-yellowing agent comprises one or more of phosphite esters or thiophosphite esters.
Further, the initiation temperature in the step 2) is 35-55 ℃, and the non-initiation temperature is-5-10 ℃; the curing time is 8-12 h.
Further, a step of air bubble pumping is also performed between the step 2) of filling the soft embedding medium and curing, namely after the soft embedding medium is filled, the mould and the section specimen in the mould are placed in a pressure chamber to be pumped for 3-5 hours until the section specimen and the embedding medium in the mould are free of air bubbles.
further, the method also comprises a process of antiseptic treatment before the freeze sawing: selecting an intact, trauma-free, fracture-free and disease-free human body specimen, filling the specimen with 15-20% by volume of formalin, infiltrating the formalin into each part of the specimen through a great vessel (femoral artery and common carotid artery), and finally soaking the specimen in 8-10% by volume of formalin preservation solution for 3-4 months to obtain the preservative-treated specimen.
Further, the process of the freeze sawing is as follows: and (3) placing the preserved specimen into a refrigerator at the temperature of-30 to-50 ℃ according to the anatomical posture, freezing for 5 to 7 days, and then sawing the frozen specimen into a sheet-shaped section specimen with the thickness of 0.2 to 0.3mm by using a high-density electric saw at the temperature of-5 to 0 ℃ to obtain the sawed section specimen.
the moisture-containing soft-covered section specimen manufactured by the manufacturing method of the moisture-containing soft-covered section specimen.
The method for manufacturing the moisture-containing soft-covered section specimen can be used for manufacturing section embedded specimens of fishes, amphibians, reptiles, birds and mammals.
The invention has the beneficial effects that:
The method for manufacturing the moisture-containing soft-covered section specimen comprises the steps of soaking the section specimen in a moisture treatment agent twice, and completely absorbing moisture on the specimen by using absorbent paper before soaking. Can lock the moisture in the section sample completely, moisture can not come out again and influence the embedding. And the water is locked in the section specimen, so that the original state of the biological tissue can be kept, and the state of the biological tissue can be truly reproduced.
According to the method for manufacturing the moisture-containing soft-package buried section specimen, the embedding agent used in the manufacturing process is the embedding agent with flexibility after curing and forming, so that the cured soft-package buried section specimen has good flexibility, can be randomly twisted to a certain angle, is not easy to crack and break, and is convenient to store and use.
according to the method for manufacturing the moisture-containing soft-covered fracture surface specimen, the embedding agent used in the manufacturing process contains UV curing resin, polyurethane acrylate, polyester acrylate, polyether acrylate, epoxy resin and the like which are cured under the action of the initiator, and the manufactured soft-covered fracture surface specimen has good flexibility and high toughness after being cured, so that the manufactured soft-covered fracture surface specimen is convenient to store and use. In addition, the anti-yellowing agent contained in the embedding agent can prevent the color of the cured and molded soft-packaged section specimen from yellowing, improve the transparency of the specimen and facilitate long-term clear observation of the specimen.
The method for manufacturing the moisture-containing soft-covered section specimen takes real organisms as the specimen, and manufactures the specimen which has the advantages of complete shape and structure, strong sense of reality, softness, difficult deformation and cracking and the like through the steps of freezing, slicing, pouring, curing and the like. The operation is simpler.
the moisture-containing soft-covered section specimen has the advantages of good flexibility, high toughness, difficulty in cracking and breaking, convenience in storage and use, contribution to observation of the specimen at the later stage, simplicity in use and operation and convenience for teachers to teach and students to learn. In addition, the soft-covered section specimen is non-toxic and odorless, can be used for multiple times to save teaching cost, can be placed in the air for a long time, is free from insects, does not have pungent smell, is easy to store, and has a complete and attractive structure.
Detailed Description
The present invention will be further described with reference to the following examples.
Example 1
The water treatment agent comprises the following components in parts by mass:
100 parts of resin, 0.2 part of initiator and 0.3 part of anti-yellowing agent.
The resin is water polyurethane acrylate.
The initiator is 2-methyl-1- [4- (methylthio) phenyl ] -2- (4-morpholinyl) -1-acetone.
The anti-yellowing agent is an epoxy resin anti-yellowing agent V76-PUv.
The soft embedding agent comprises the following components in parts by mass:
100 parts of a UV curable resin having flexibility after UV curing, 0.6 part of a photoinitiator, and 0.3 part of an anti-yellowing agent.
The UV curable resin having flexibility after UV curing is aqueous urethane acrylate.
the anti-yellowing agent comprises a phosphite.
The hardness of the UV-curable resin after curing is Shore A60.
The method for manufacturing the moisture-containing soft-covered section specimen comprises the following steps:
1) And (3) antiseptic treatment: selecting an intact, trauma-free, fracture-free and disease-free human body specimen, filling the specimen with 15% by volume of formalin, infiltrating the formalin into each part of the specimen through large blood vessels (femoral artery and common carotid artery), and finally soaking the specimen in 10% by volume of formalin preservation solution for 4 months to obtain the preservative-treated specimen.
2) And (3) performing freeze sawing: placing the specimen subjected to the preservative treatment in the step 1) into a refrigerator at the temperature of-30 ℃ according to the anatomical posture, freezing for 5 days, and then sawing the frozen specimen into a sheet-shaped section specimen with the thickness of 0.3mm by using a high-density electric saw at the temperature of-4 ℃ to obtain the sawed section specimen.
3) Trimming and bleaching: trimming the sawn section specimen in the step 2) to be clean and tidy, and bleaching the section specimen in 8% hydrogen peroxide for 3d to obtain a bleached section specimen.
4) Preparing a water treatment agent: and (3) mixing and uniformly stirring the resin, the initiator and the anti-yellowing agent according to the formula ratio to obtain the anti-yellowing agent.
5) Water treatment: washing the bleached section specimen in the step 3) for 2 days, completely washing, completely absorbing water on the surface of the section specimen by using a water absorption towel, then placing the section specimen in resin for soaking for 8min, then taking out the section specimen to naturally solidify the section specimen, then placing the section specimen in resin for soaking for 10min, taking out after soaking is finished, waiting for solidification, and controlling the temperature of soaking twice and solidification at 30 ℃.
6) Manufacturing a mold: and manufacturing a mold by using glass according to the size of the specimen.
7) Preparing an embedding agent: and (3) uniformly mixing the UV curing resin, the photoinitiator and the anti-yellowing agent at a non-initiation temperature, namely-5 ℃ to obtain the soft embedding agent.
8) Embedding: placing the dehydrated and degreased section specimen in the step 5) into a mould, then filling the soft embedding agent into the mould, moving the mould into a stainless steel pressure cabin after filling, and exhausting for 3h until no bubble exists on the filled section specimen, thereby obtaining the filled section specimen.
9) And (3) curing: and (3) placing the filled section specimen in the step 8) into an ultraviolet incubator UV curing box for curing for 8h, wherein the temperature set by the ultraviolet incubator UV curing box is the initiation temperature, namely 35 ℃, and trimming the section specimen clean and tidy after curing is finished to obtain the finished product.
The moisture-containing buried fracture surface specimen of the present example, that is, the moisture-containing buried fracture surface specimen produced by the method for producing the moisture-containing buried fracture surface specimen of the present example.
Example 2
The water treatment agent comprises the following components in parts by mass:
100 parts of resin, 0.6 part of initiator and 0.1 part of anti-yellowing agent.
The resin is water-based epoxy acrylate.
The initiator is 2-methyl-1- (4-methylthiophenyl) -2-morpholinyl-1-acetone.
The anti-yellowing agent is an ultraviolet absorbent UV-196.
The soft embedding agent comprises the following components in parts by mass:
100 parts of a UV curable resin having flexibility after UV curing, 0.5 part of a photoinitiator, and 0.2 part of an anti-yellowing agent.
The UV curable resin having flexibility after UV curing is polyurethane, and the polyurethane is urethane acrylate.
The anti-yellowing agent comprises one or more of thiophosphites.
The hardness of the UV-curable resin after curing is Shore A60.
The method for manufacturing the moisture-containing soft-covered section specimen comprises the following steps:
1) And (3) antiseptic treatment: selecting an intact, trauma-free, fracture-free and disease-free human body specimen, filling the specimen with 20% by volume of formalin, infiltrating the formalin into each part of the specimen through large blood vessels (femoral artery and common carotid artery), and finally soaking the specimen in 9% by volume of formalin preservation solution for 3 months to obtain the preservative-treated specimen.
2) And (3) performing freeze sawing: placing the specimen subjected to preservative treatment in the step 1) into a refrigerator at the temperature of-40 ℃ according to the anatomical posture, freezing for 6 days, and then sawing the frozen specimen into a sheet-shaped section specimen with the thickness of 0.2mm by using a high-density electric saw at the temperature of-5 ℃ to obtain the sawed section specimen.
3) Trimming and bleaching: trimming the sawn section specimen in the step 2) to be clean and tidy, and bleaching the section specimen in 8% hydrogen peroxide for 3d to obtain a bleached section specimen.
4) Preparing a water treatment agent: and (3) mixing and uniformly stirring the resin, the initiator and the anti-yellowing agent according to the formula ratio to obtain the anti-yellowing agent.
5) Water treatment: washing the bleached section specimen in the step 3) for 2 days, completely washing, completely absorbing water on the surface of the section specimen by using a water absorption towel, then placing the section specimen in resin for soaking for 7min, then taking out the section specimen to naturally solidify the section specimen, then placing the section specimen in resin for soaking for 5min, taking out after soaking is finished, waiting for solidification, and controlling the temperature of soaking twice and solidification at 20 ℃.
6) Manufacturing a mold: and manufacturing a mold by using glass according to the size of the specimen.
7) Preparing an embedding agent: and (3) uniformly mixing the UV curing resin, the photoinitiator and the anti-yellowing agent at a non-initiation temperature, namely 10 ℃, so as to obtain the soft embedding agent.
8) Embedding: placing the dehydrated and degreased section specimen in the step 5) into a mould, then filling the soft embedding agent into the mould, moving the mould into a stainless steel pressure cabin after filling, and exhausting for 4h until no bubble exists on the filled section specimen, thereby obtaining the filled section specimen.
9) And (3) curing: and (3) placing the section specimen after perfusion in the step 8) into an ultraviolet incubator UV curing box for curing for 8h, wherein the temperature set by the ultraviolet incubator UV curing box is the initiation temperature, namely 40 ℃, and trimming the section specimen clean and tidy after curing is finished to obtain the section specimen.
The moisture-containing buried fracture surface specimen of the present example, that is, the moisture-containing buried fracture surface specimen produced by the method for producing the moisture-containing buried fracture surface specimen of the present example.
Example 3
the water treatment agent comprises the following components in parts by mass:
100 parts of resin, 0.8 part of initiator and 0.4 part of anti-yellowing agent.
The resin is one or more of waterborne polyester acrylate.
the initiator is 2-methyl-1- (4-methylthiophenyl) -2-morpholine-1-propanone IHT-PI 907).
the anti-yellowing agent is an epoxy resin anti-yellowing agent V76-PUv.
The soft embedding agent comprises the following components in parts by mass:
100 parts of a UV curable resin having flexibility after UV curing, 0.2 part of a photoinitiator, and 0.6 part of an anti-yellowing agent.
The UV curable resin having flexibility after UV curing is polyester acrylate.
The anti-yellowing agent comprises one or more of thiophosphites.
The hardness of the UV-curable resin after curing is Shore A60.
the method for manufacturing the moisture-containing soft-covered section specimen comprises the following steps:
1) And (3) antiseptic treatment: selecting an intact, trauma-free, fracture-free and disease-free human body specimen, filling the specimen with formalin with the volume fraction of 18%, penetrating the formalin to each part of the specimen through a great vessel (femoral artery and common carotid artery), and finally soaking the specimen in formalin preservation solution with the volume fraction of 8% for 3 months to obtain the preservative-treated specimen.
2) and (3) performing freeze sawing: placing the specimen subjected to the preservative treatment in the step 1) into a refrigerator at the temperature of-50 ℃ according to the anatomical posture, freezing for 7 days, and then sawing the frozen specimen into a sheet-shaped section specimen with the thickness of 0.3mm by using a high-density electric saw at the temperature of 0 ℃ to obtain the sawed section specimen.
3) Trimming and bleaching: trimming the sawn section specimen in the step 2) to be clean and tidy, and bleaching the section specimen in 8% hydrogen peroxide for 3d to obtain a bleached section specimen.
4) Preparing a water treatment agent: and (3) mixing and uniformly stirring the resin, the initiator and the anti-yellowing agent according to the formula ratio to obtain the anti-yellowing agent.
5) Water treatment: washing the bleached section specimen in the step 3) for 2 days, completely washing, completely absorbing water on the surface of the section specimen by using a water absorption towel, then placing the section specimen in resin for soaking for 5min, then taking out the section specimen to naturally solidify the section specimen, then placing the section specimen in resin for soaking for 6min, taking out after soaking is finished, waiting for solidification, and controlling the temperature of soaking twice and solidification at 20 ℃.
6) Manufacturing a mold: and manufacturing a mold by using glass according to the size of the specimen.
7) Preparing an embedding agent: and (3) uniformly mixing the UV curing resin, the photoinitiator and the anti-yellowing agent at a non-initiation temperature, namely-5 ℃ to obtain the soft embedding agent.
8) Embedding: placing the dehydrated and degreased section specimen in the step 5) into a mould, then filling the soft embedding agent into the mould, moving the mould into a stainless steel pressure cabin after filling, and exhausting for 5 hours until no bubble exists on the filled section specimen, thereby obtaining the filled section specimen.
9) and (3) curing: and (3) placing the filled section specimen in the step 8) into an ultraviolet incubator UV curing box for curing for 8h, wherein the temperature set by the ultraviolet incubator UV curing box is 55 ℃ which is the initiation temperature, and trimming the section specimen to be clean and tidy after curing is finished.
the moisture-containing buried fracture surface specimen of the present example, that is, the moisture-containing buried fracture surface specimen produced by the method for producing the moisture-containing buried fracture surface specimen of the present example.
Example 4
the water treatment agent comprises the following components in parts by mass:
100 parts of resin, 0.5 part of initiator and 0.5 part of anti-yellowing agent.
The resin is water-based urethane acrylate.
The initiator is 2-methyl-1- [4- (methylthio) phenyl ] -2- (4-morpholinyl) -1-acetone.
The anti-yellowing agent is an ultraviolet absorbent UV-196.
The soft embedding agent comprises the following components in parts by mass:
100 parts of a UV curable resin having flexibility after UV curing, 0.8 part of a photoinitiator, and 0.5 part of an anti-yellowing agent.
The UV curable resin having flexibility after UV curing is polyether acrylate.
The anti-yellowing agent includes a thiophosphite.
the hardness of the UV-curable resin after curing is Shore A60.
the method for manufacturing the moisture-containing soft-covered section specimen comprises the following steps:
1) And (3) antiseptic treatment: selecting an intact, trauma-free, fracture-free and disease-free human body specimen, filling the specimen with 15% by volume of formalin, infiltrating the formalin into each part of the specimen through large blood vessels (femoral artery and common carotid artery), and finally soaking the specimen in 8% by volume of formalin preservation solution for 4 months to obtain the preservative-treated specimen.
2) And (3) performing freeze sawing: placing the specimen subjected to preservative treatment in the step 1) into a refrigerator at minus 30 ℃ according to the anatomical posture, freezing for 5 days, and then sawing the frozen specimen into a sheet-shaped section specimen with the thickness of 0.2mm by using a high-density electric saw at the temperature of minus 3 ℃ to obtain the sawed section specimen.
3) Trimming and bleaching: trimming the sawn section specimen in the step 2) to be clean and tidy, and bleaching the section specimen in 8% hydrogen peroxide for 3d to obtain a bleached section specimen.
4) Preparing a water treatment agent: and (3) mixing and uniformly stirring the resin, the initiator and the anti-yellowing agent according to the formula ratio to obtain the anti-yellowing agent.
5) Water treatment: washing the bleached section specimen in the step 3) for 2 days, completely washing, completely absorbing water on the surface of the section specimen by using a water absorption towel, then placing the section specimen in resin for soaking for 6min, then taking out the section specimen to naturally solidify the section specimen, then placing the section specimen in resin for soaking for 7min, taking out after soaking is finished, waiting for solidification, and controlling the temperature of soaking twice and solidification at 25 ℃.
6) manufacturing a mold: and manufacturing a mold by using glass according to the size of the specimen.
7) Preparing an embedding agent: and (3) uniformly mixing the UV curing resin, the photoinitiator and the anti-yellowing agent at a non-initiation temperature, namely 0 ℃, so as to obtain the soft embedding agent.
8) Embedding: placing the dehydrated and degreased section specimen in the step 5) into a mould, then filling the soft embedding agent into the mould, moving the mould into a stainless steel pressure cabin after filling, and exhausting for 4h until no bubble exists on the filled section specimen, thereby obtaining the filled section specimen.
9) And (3) curing: and (3) placing the filled section specimen in the step 8) into an ultraviolet incubator UV curing box for curing for 8h, wherein the temperature set by the ultraviolet incubator UV curing box is the initiation temperature, namely 35 ℃, and trimming the section specimen clean and tidy after curing is finished to obtain the finished product.
The moisture-containing buried fracture surface specimen of the present example, that is, the moisture-containing buried fracture surface specimen produced by the method for producing the moisture-containing buried fracture surface specimen of the present example.
Example 5
The water treatment agent comprises the following components in parts by mass:
100 parts of resin, 0.4 part of initiator and 0.2 part of anti-yellowing agent.
The resin is water-based epoxy acrylate.
The initiator is 2-methyl-1- (4-methylthiophenyl) -2-morpholine-1-propanone IHT-PI 907).
The anti-yellowing agent is an epoxy resin anti-yellowing agent V76-PUv.
The soft embedding agent comprises the following components in parts by mass:
100 parts of a UV curable resin having flexibility after UV curing, 0.7 part of a photoinitiator, and 0.4 part of an anti-yellowing agent.
The UV curable resin having flexibility after UV curing is aqueous urethane acrylate.
The anti-yellowing agent includes a thiophosphite.
The hardness of the UV-curable resin after curing is Shore A60.
The method for manufacturing the moisture-containing soft-covered section specimen comprises the following steps:
1) And (3) antiseptic treatment: selecting an intact, trauma-free, fracture-free and disease-free human body specimen, filling the specimen with 16% by volume of formalin, infiltrating the formalin into each part of the specimen through large blood vessels (femoral artery and common carotid artery), and finally soaking the specimen in 9% by volume of formalin preservation solution for 3 months to obtain the preservative-treated specimen.
2) And (3) performing freeze sawing: placing the specimen subjected to preservative treatment in the step 1) into a refrigerator at the temperature of-40 ℃ according to the anatomical posture, freezing for 6 days, and then sawing the frozen specimen into a sheet-shaped section specimen with the thickness of 0.2mm by using a high-density electric saw at the temperature of-2 ℃ to obtain the sawed section specimen.
3) Trimming and bleaching: trimming the sawn section specimen in the step 2) to be clean and tidy, and bleaching the section specimen in 8% hydrogen peroxide for 3d to obtain a bleached section specimen.
4) Preparing a water treatment agent: and (3) mixing and uniformly stirring the resin, the initiator and the anti-yellowing agent according to the formula ratio to obtain the anti-yellowing agent.
5) water treatment: washing the bleached section specimen in the step 3) for 2 days, completely washing, completely absorbing water on the surface of the section specimen by using a water absorption towel, then placing the section specimen in resin for soaking for 10min, then taking out the section specimen to naturally solidify the section specimen, then placing the section specimen in resin for soaking for 8min, taking out after soaking is finished, waiting for solidification, and controlling the temperature of soaking twice and solidification at 30 ℃.
6) Manufacturing a mold: and manufacturing a mold by using glass according to the size of the specimen.
7) Preparing an embedding agent: and (3) uniformly mixing the UV curing resin, the photoinitiator and the anti-yellowing agent at a non-initiation temperature, namely 5 ℃, so as to obtain the soft embedding agent.
8) Embedding: placing the dehydrated and degreased section specimen in the step 5) into a mould, then filling the soft embedding agent into the mould, moving the mould into a stainless steel pressure cabin after filling, and exhausting for 3h until no bubble exists on the filled section specimen, thereby obtaining the filled section specimen.
9) And (3) curing: and (3) placing the section specimen after perfusion in the step 8) into an ultraviolet incubator UV curing box for curing for 8h, wherein the temperature set by the ultraviolet incubator UV curing box is the initiation temperature, namely 40 ℃, and trimming the section specimen clean and tidy after curing is finished to obtain the section specimen.
The moisture-containing buried fracture surface specimen of the present example, that is, the moisture-containing buried fracture surface specimen produced by the method for producing the moisture-containing buried fracture surface specimen of the present example.
Claims (10)
1. The method for manufacturing the moisture-containing soft-covered section specimen is characterized by comprising the following steps of:
1) Pretreatment of biological tissues: freezing and sawing a biological specimen into a sheet-shaped section specimen, soaking the section specimen in a water treatment agent for water treatment, and obtaining a pretreated section specimen after soaking;
2) Placing the pretreated section specimen in the step 1) in a mould, and pouring a soft embedding agent into the mould at a non-initiation temperature, wherein the soft embedding agent has flexibility after being cured; and (5) curing at the initiation temperature after the completion of the pouring, thus obtaining the product.
2. The method for manufacturing the moisture-containing soft-buried fracture surface specimen according to claim 1, wherein the moisture treatment agent in the step 1) comprises a resin and an initiator, and the mass ratio of the resin to the initiator is 100: 0.2-100: 0.8.
3. the method for manufacturing the soft buried fracture surface specimen containing water according to claim 2, wherein the resin is one or more of water-based urethane acrylate, water-based epoxy acrylate and water-based polyester acrylate; the initiator is one or more of 2-methyl-1- [4- (methylthio) phenyl ] -2- (4-morpholinyl) -1-acetone, 2-methyl-1- (4-methylthiophenyl) -2-morpholinyl-1-acetone and 2-methyl-1- (4-methylthiophenyl) -2-morpholine-1-acetone IHT-PI 907).
4. The method for manufacturing the moisture-containing soft-buried fracture surface specimen according to claim 2 or 3, wherein the moisture treatment agent further contains an anti-yellowing agent, and the mass ratio of the resin to the anti-yellowing agent is 100: 0.1-100: 0.5.
5. The method for manufacturing the moisture-containing soft-packed fracture surface specimen according to claim 4, wherein the anti-yellowing agent is an epoxy resin anti-yellowing agent V76-PUv or an ultraviolet absorber UV-196.
6. The method for manufacturing the moisture-containing soft-covered section specimen according to claim 1 or 2, wherein the moisture treatment comprises the steps of immersing the section specimen in the resin for 5-10 min, taking out the section specimen, naturally curing the section specimen, immersing the section specimen in the resin for 5-10 min, and controlling the temperature of the two immersion and curing to be 20-30 ℃.
7. The method for manufacturing the moisture-containing soft-buried fracture surface specimen according to claim 1, wherein the soft embedding medium comprises the following components in parts by mass: 100 parts of UV curing resin with flexibility after UV curing and 0.2-0.8 part of photoinitiator, wherein the hardness of the UV curing resin after curing is Shore A60-A90.
8. The method for manufacturing the soft-covered section specimen with moisture according to claim 7, wherein the UV curing resin is one or more of waterborne polyurethane acrylate, waterborne epoxy acrylate, waterborne polyester acrylate, polyurethane, acrylate and epoxy resin, the polyurethane is polyurethane acrylate, and the acrylate is one or more of polyester acrylate or polyether acrylate; the photoinitiator is one or more of 2-methyl-1- [4- (methylthio) phenyl ] -2- (4-morpholinyl) -1-acetone, 2-methyl-1- (4-methylthiophenyl) -2-morpholinyl-1-acetone and 2-methyl-1- (4-methylthiophenyl) -2-morpholine-1-acetone IHT-PI 907).
9. The method for manufacturing the moisture-containing soft-covered fracture surface specimen according to claim 7, wherein the soft embedding medium further comprises 0.2-0.6 parts by mass of an anti-yellowing agent, and the anti-yellowing agent comprises one or more of phosphite esters or thiophosphite esters.
10. The moisture-containing buried fracture surface specimen produced by the method for producing a moisture-containing buried fracture surface specimen according to claim 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910905312.XA CN110558308B (en) | 2019-09-24 | 2019-09-24 | Moisture-containing soft-buried section specimen and manufacturing method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910905312.XA CN110558308B (en) | 2019-09-24 | 2019-09-24 | Moisture-containing soft-buried section specimen and manufacturing method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110558308A true CN110558308A (en) | 2019-12-13 |
| CN110558308B CN110558308B (en) | 2021-07-27 |
Family
ID=68782185
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910905312.XA Active CN110558308B (en) | 2019-09-24 | 2019-09-24 | Moisture-containing soft-buried section specimen and manufacturing method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110558308B (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105010302A (en) * | 2015-07-08 | 2015-11-04 | 南宁千年工艺有限公司 | Method for preparing resin-embedded water-containing animal specimen |
| CN109523873A (en) * | 2019-01-22 | 2019-03-26 | 河南中博科技有限公司 | Soft Roll buries cross-section specimen and preparation method thereof |
| US20190151511A1 (en) * | 2015-12-04 | 2019-05-23 | Colorado State University Research Foundation | Soft tissue mimetics and thermoplastic elastomer hydrogels |
-
2019
- 2019-09-24 CN CN201910905312.XA patent/CN110558308B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105010302A (en) * | 2015-07-08 | 2015-11-04 | 南宁千年工艺有限公司 | Method for preparing resin-embedded water-containing animal specimen |
| US20190151511A1 (en) * | 2015-12-04 | 2019-05-23 | Colorado State University Research Foundation | Soft tissue mimetics and thermoplastic elastomer hydrogels |
| CN109523873A (en) * | 2019-01-22 | 2019-03-26 | 河南中博科技有限公司 | Soft Roll buries cross-section specimen and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110558308B (en) | 2021-07-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109523873B (en) | Soft-covered section specimen and manufacturing method thereof | |
| US4278701A (en) | Animal and vegetal tissues permanently preserved by synthetic resin impregnation | |
| DE69901612D1 (en) | METHOD FOR PRODUCING ELASTIC DEHYDRATED IMPLANT TISSUE | |
| CN109619086A (en) | Animal muscle is plasticized sample and preparation method thereof | |
| CN103987392A (en) | Method for preparing decellularized tissue product, and graft provided with decellularized tissue product | |
| CN104041482B (en) | Manufacturing method of lepidopteron plastinated specimens | |
| Saeed et al. | Mummification to plastination | |
| ATE112932T1 (en) | METHODS OF PRESERVING ORGANS. | |
| CN110558308B (en) | Moisture-containing soft-buried section specimen and manufacturing method thereof | |
| Tiedemann et al. | The technique of heart plastination | |
| Șora | The general protocol for the s10 technique | |
| Jenssen | Conservation of wet organic artefacts excluding wood | |
| CA2755907A1 (en) | Collagen implant | |
| JP2006126176A (en) | Method for producing decalcified hard tissue sample | |
| CN110637806A (en) | Biological plasticizing specimen opened from back and displaying viscera position relation and preparation method thereof | |
| Ameko et al. | Plastination of some cow and ram organs in Ghana for use as teaching aids | |
| JP2012501187A (en) | A high-throughput and non-invasive method for vitrifying pig embryos | |
| JPS6236301A (en) | Production of fish specimen | |
| KR101284530B1 (en) | Exchange reagent for biological permanent specimen and use of thereof | |
| CN109377846B (en) | Method for making human anatomy viscera specimen | |
| CN115005196A (en) | Manufacturing method of small-sized mammal meridian acupoint section embedding specimen | |
| RU2689950C1 (en) | Method for preserving bone anatomical preparations | |
| CN114868735A (en) | Method for manufacturing display specimen by combining plasticized specimen and embedded specimen | |
| CN113575570A (en) | Method for manufacturing plasticized specimen not easy to mildew | |
| CN116548431B (en) | Method for preparing large fungus original state specimen by A-B mixed crystal dropping glue |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |