CN1317235A - Method for plasticizing biological samples - Google Patents
Method for plasticizing biological samples Download PDFInfo
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- CN1317235A CN1317235A CN 00105847 CN00105847A CN1317235A CN 1317235 A CN1317235 A CN 1317235A CN 00105847 CN00105847 CN 00105847 CN 00105847 A CN00105847 A CN 00105847A CN 1317235 A CN1317235 A CN 1317235A
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- plasticizing
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- dehydrating agent
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Abstract
A method for plasticizing the biologic samples includes dewatering said sample, immersing in mixed resin solution, dripdrying, coating catalyst, reaction, drying and solidification. The resultant biologic sample features complete state of tissue cels, high softness and actuality, and long storage period.
Description
The present invention relates to a kind of method of making biological sample, particularly a kind of resin mixture liquor of utilizing is main medium, and will cement out up to the body fluid more than 70% in the biological sample, and then realizes the method for its sample plasticizing.
General biological sample commonly used utilizes dipped into formalin anticorrosion to reach the purpose of long preservation more.At first, use formalin the blood replacing in the biological sample to be come out, impregnated in the formalin liquid biological sample is whole again in the perfusion mode.Yet, common method has following shortcoming: first, biological sample is soaked in the formalin solution for a long time, its grease can be dissolved out, cause the formalin solution muddiness, therefore must the periodic replacement formalin solution, and formalin solution can only reach temporary preservation, and the biological sample long period of soaking can be damaged.The second, biological sample is soaked in the formalin solution, can only watch and can not directly touch, and this will have a greatly reduced quality for results of learning.The 3rd, formalin is a kind of poisonous solution, and long-term contact can cause the infringement of human body, insalubrity.The 4th, biological sample is after the formalin long period of soaking, and its histocyte all wrecks, can't study use.
Method for plasticizing biological samples of the present invention can solve above-mentioned shortcoming effectively, can not only preserve biological sample really, more can improve the value of biological sample.
Method for plasticizing biological samples of the present invention, its main purpose are properly to preserve biological sample, and via the biological sample that plasticizing is finished, not only its outward appearance or histocyte can remain intact state, and overall appearance has pliability and possesses the sense of reality.
Method for plasticizing biological samples of the present invention, its second purpose is to improve the value of biological sample, this biological sample not only can be used as observes teaching usefulness, more can actually provide to dissect and use, watch research in microscopically behind the longitudinal section, can get an original and complete tissue and a cell, therefore have multiple value.
The biological sample that method for plasticizing biological samples of the present invention, its 3rd purpose are to plastify employed chemicals and plasticizing after finishing all can the safe touch and the toxicity of not having, and can not damage human body and direct contact site skin.
Method for plasticizing biological samples of the present invention, be that to utilize resin mixture liquor be main medium, and will cement out up to the body fluid more than 70% in the biological sample, and then realize the method for its sample plasticizing, its making step includes: dehydration, be soaked in the resin mixture liquor fully, leave standstill and drain, painting catalyst, leave standstill steps such as reaction and dry solidification.Biological sample after plasticizing is finished, its outward appearance and histocyte all can remain intact state, and have the pliability and the sense of reality.
To other purposes of the present invention and effect, after being further described in conjunction with following accompanying drawing, the auditor is easier to understand.
Fig. 1 is the flow chart of the present invention's first preferred embodiment,
Fig. 2 is the flow chart of the present invention's second preferred embodiment.
Method for plasticizing biological samples of the present invention, it is main medium that system utilizes resin mixture liquor, and will cement out up to the body fluid more than 70% in the biological sample, and then realize the method for its sample plasticizing, its making step includes: dehydration, be soaked in the resin mixture liquor fully, leave standstill and drain, painting catalyst, leave standstill reaction and steps such as dry solidification, wherein, biological sample of the present invention, except that can directly fresh biological sample being made, also can be a biological sample that had soaked formalin and make.
As shown in Figure 1, the present invention plastifies biological sample, and its making step is as follows:
A. biological sample be impregnated in the dehydrating agent and dewater;
B. will impregnated in the resin mixture liquor by the biological sample that obtains in the steps A;
C. leave standstill and drain;
D. evenly be coated with or be sprayed on catalyzer on the surface of biological sample;
E. leave standstill reaction;
F. dry solidification.
In steps A of the present invention, biological sample be impregnated in the middle dehydration of dehydrating agent (its dehydrating agent preferred embodiment is a pure acetone) after 1-2 days, this biological sample is taken out, impregnated in another identical dehydrating agent (pure acetone) middle 1-2 days, carry out repeatedly, to the last still reach 95% when above through the dehydrating agent concentration of dipping behind the biological sample, steps A can stop.In this steps A, be impregnated in the dehydrating agent and dewater, its purpose is with this dehydrating agent the body fluid moisture in this biological sample or other residual liquid compositions (as formalin solution) to be cemented out, prevent this biological sample atrophy, in steps A, replace fully for asking, look the concentration of dehydrating agent and implement repeatedly the step that biological sample impregnated in dehydrating agent, the body fluid in this sample can be displaced fully.
In step B, will impregnated in the resin mixture liquor about 2 days by the biological sample that obtains in the steps A, this biological sample is taken out, impregnated in another same resin mixed liquor about 2 days again, carry out repeatedly repeatedly; Wherein, this resin mixture liquor is mixed by polymer resin and polymerizer.The necessary thorough impregnation of biological sample does not make this biological sample be exposed in the air in mixed liquor, directly evaporates into the air from this biological sample to avoid this dehydrating agent, and makes this biological sample rotten.
In step C, by taking out this biological sample in the resin mixture liquor, it was statically placed in the air about 8-12 hour, make it drain unnecessary resin mixture liquor (it is different or required and omit that this step also can be complied with its biological sample).
In step D, catalyzer is evenly coated on the biological sample surface, or catalyzer evenly is sprayed on the sample surface with sprayer.
In step e, will be statically placed in a confined space by the biological sample that obtains among the step D, it is reacted.
At last, in step F, this biological sample is taken out, place the closed container a few days to solidify to reach fully.
As shown in Figure 2, plastify the making step that method plastifies the biological sample that soaked formalin solution with the present invention:
A. the biological sample water flushing that will soak formalin solution is to accumulate the formalin of covering on the flush away biological sample surface;
B. biological sample be impregnated in the dehydrating agent and dewater;
C. will impregnated in the resin mixture liquor by the biological sample that obtains among the step b;
D. leave standstill and drain;
E. catalyzer is evenly coated on the biological sample surface;
F. leave standstill reaction;
G. dry solidification.
In step a of the present invention, continue the biological sample one day that formalin solution had been soaked in the water flushing, with flush away formalin; In step b of the present invention, biological sample be impregnated in the middle dehydration of dehydrating agent (preferred embodiment of this dehydrating agent is a pure acetone) after 1-2 days, this biological sample is taken out, impregnated in another identical dehydrating agent (pure acetone) middle 1-2 days, carry out repeatedly, to the last still reach 95% when above through the dehydrating agent concentration of dipping behind the biological sample, step b can stop; In step b, be impregnated in the dehydrating agent and dewater, its purpose is with this dehydrating agent the body fluid moisture in this biological sample or other residual liquid compositions (as formalin solution) to be cemented out, and prevents this biological sample atrophy.For asking displacement fully, look the concentration of dehydrating agent and implement repeatedly the step that biological sample impregnated in dehydrating agent in step b, the body fluid in this sample can be displaced fully.
In step c, will impregnated in the resin mixture liquor about 2 days by the biological sample that produces among the step b, this biological sample is taken out, impregnated in another same resin mixed liquor about 2 days again, carry out repeatedly repeatedly, can be full of in the biological sample fully until resin mixture liquor.Wherein, this resin mixture liquor is mixed by polymer resin and polymerizer.The necessary thorough impregnation of this biological sample does not make this biological sample be exposed in the air in mixed liquor, directly evaporates into the air from this biological sample to avoid this dehydrating agent, and makes this biological sample rotten.
In steps d, by taking out this biological sample in the resin mixture liquor, it was statically placed in the air about 8-12 hour, make it drain unnecessary resin mixture liquor (it is different or required and omit that this step also can be complied with its biological sample).
In step e, catalyzer is evenly coated on the biological sample surface, or catalyzer evenly is sprayed on the sample surface with sprayer.
In step f, will be statically placed in a confined space by the biological sample that step e obtains, it is reacted.
At last, in step g, this biological sample is taken out, place the closed container a few days to solidify to reach fully.
Below the present invention has been done detailed description, but the above description of doing only is preferred embodiment of the present invention, can not be used for limiting scope of the invention process.Be that all equivalences of doing according to the present patent application scope change and modify etc., the scope that all should still belong to patent of the present invention and covered.
Claims (6)
1. the plasticizing method of a biological sample, this method is made into the plasticizing biological sample with general biology, and its making step includes:
A. biological sample be impregnated in the dehydrating agent and dewater;
B. will impregnated in the resin mixture liquor by the biological sample that obtains in the steps A;
C. leave standstill and drain;
D. evenly be coated with or be sprayed on catalyzer on the surface of biological sample;
E. leave standstill reaction;
F. dry solidification.
2. method for plasticizing biological samples as claimed in claim 1, wherein said resin mixture liquor can be the mixture of polymer resin and polymerizer.
3. method for plasticizing biological samples as claimed in claim 1, wherein said dehydrating agent can be pure acetone.
4. method for plasticizing biological samples, this method is made into the plasticizing biological sample with general biology, and its making step includes:
A. the biological sample water flushing that will soak formalin solution is to accumulate the formalin solution of covering on the flush away surface;
B. the biological sample of step a be impregnated in the dehydrating agent and dewater;
C. the biological sample with step b impregnated in the resin mixture liquor;
D. leave standstill and drain;
E. evenly be coated with or be sprayed on catalyzer on the biological sample surface;
F. leave standstill reaction;
G. dry solidification.
5. method for plasticizing biological samples as claimed in claim 4, wherein said tree refer to that mixed liquor can be the mixture of polymer resin and polymerizer.
6. method for plasticizing biological samples as claimed in claim 4, wherein dehydrating agent can be pure acetone.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00105847 CN1317235A (en) | 2000-04-11 | 2000-04-11 | Method for plasticizing biological samples |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00105847 CN1317235A (en) | 2000-04-11 | 2000-04-11 | Method for plasticizing biological samples |
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| CN1317235A true CN1317235A (en) | 2001-10-17 |
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| CN 00105847 Pending CN1317235A (en) | 2000-04-11 | 2000-04-11 | Method for plasticizing biological samples |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103053510A (en) * | 2013-01-31 | 2013-04-24 | 南京医科大学 | Simple manufacturing method for long-term preserving softened human anatomy viscous specimen |
| CN103700306A (en) * | 2013-12-12 | 2014-04-02 | 南阳医学高等专科学校 | Fabrication method and puncture method of puncture specimen |
| CN105010302A (en) * | 2015-07-08 | 2015-11-04 | 南宁千年工艺有限公司 | Method for preparing resin-embedded water-containing animal specimen |
| CN108566946A (en) * | 2018-06-04 | 2018-09-25 | 陕西科技大学 | A kind of plant specimen production method |
| CN108849862A (en) * | 2018-06-19 | 2018-11-23 | 郑州百草文化传播有限公司 | A kind of plant plasticizing Slide processing |
| CN108922356A (en) * | 2018-07-13 | 2018-11-30 | 河南中博科技有限公司 | Human body is plasticized nursing teaching sample and preparation method thereof |
| CN109619086A (en) * | 2019-01-17 | 2019-04-16 | 河南中博科技有限公司 | Animal muscle is plasticized sample and preparation method thereof |
| CN111213632A (en) * | 2019-11-19 | 2020-06-02 | 长春中医药大学 | Method and equipment for making animal medicine epoxy resin specimen |
| CN113180033A (en) * | 2021-04-28 | 2021-07-30 | 黄倞朗 | Preparation method of small biological specimen |
-
2000
- 2000-04-11 CN CN 00105847 patent/CN1317235A/en active Pending
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103053510A (en) * | 2013-01-31 | 2013-04-24 | 南京医科大学 | Simple manufacturing method for long-term preserving softened human anatomy viscous specimen |
| CN103053510B (en) * | 2013-01-31 | 2014-10-15 | 南京医科大学 | Simple Manufacturing Method for Long-Term Preserving Softened Human Anatomy Viscous Specimen |
| CN103700306A (en) * | 2013-12-12 | 2014-04-02 | 南阳医学高等专科学校 | Fabrication method and puncture method of puncture specimen |
| CN103700306B (en) * | 2013-12-12 | 2015-09-09 | 南阳医学高等专科学校 | The method for making of puncture sample and piercing method |
| CN105010302A (en) * | 2015-07-08 | 2015-11-04 | 南宁千年工艺有限公司 | Method for preparing resin-embedded water-containing animal specimen |
| CN105010302B (en) * | 2015-07-08 | 2017-10-10 | 南宁千年工艺股份有限公司 | A kind of preparation method of the aqueous zoological specimens of resin embedding |
| CN108566946A (en) * | 2018-06-04 | 2018-09-25 | 陕西科技大学 | A kind of plant specimen production method |
| CN108849862A (en) * | 2018-06-19 | 2018-11-23 | 郑州百草文化传播有限公司 | A kind of plant plasticizing Slide processing |
| CN108922356A (en) * | 2018-07-13 | 2018-11-30 | 河南中博科技有限公司 | Human body is plasticized nursing teaching sample and preparation method thereof |
| CN109619086A (en) * | 2019-01-17 | 2019-04-16 | 河南中博科技有限公司 | Animal muscle is plasticized sample and preparation method thereof |
| CN111213632A (en) * | 2019-11-19 | 2020-06-02 | 长春中医药大学 | Method and equipment for making animal medicine epoxy resin specimen |
| CN113180033A (en) * | 2021-04-28 | 2021-07-30 | 黄倞朗 | Preparation method of small biological specimen |
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