CN105010302B - A kind of preparation method of the aqueous zoological specimens of resin embedding - Google Patents
A kind of preparation method of the aqueous zoological specimens of resin embedding Download PDFInfo
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- CN105010302B CN105010302B CN201510396645.6A CN201510396645A CN105010302B CN 105010302 B CN105010302 B CN 105010302B CN 201510396645 A CN201510396645 A CN 201510396645A CN 105010302 B CN105010302 B CN 105010302B
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Abstract
The invention provides a kind of preparation method of the aqueous zoological specimens of resin embedding, comprise the following steps：Preservative treatment, the sample after being handled are carried out to aqueous zoological specimens；It will be surface-treated in sample immersion protection resin liquid after the processing, obtain treating specimen embedding；The protection resin liquid includes component A and B component, and the component A is PEPA, and its viscosity is 250 600cps；The B component is isocyanates, and its viscosity is 150 500cps；The weight ratio of the component A and B component is 1 ~ 2：1；Treat that specimen embedding carries out resin embedding to described.Using technical scheme, the specimen morphology color being embedded in resin is true to nature, the quality problems such as sample surface does not haze；Embeding resin does not haze, turbid phenomenon；Resin transparent degree is high, can be clearly observable sample each details；Use for a long time, resin does not turn yellow, and sample is not corrupt.
Description
Technical field
The present invention relates to the making side of a kind of preparation method of sample, more particularly to a kind of aqueous zoological specimens of resin embedding
Method.
Background technology
With the aqueous sample of resin embedding, sample is encapsulated among solid transparent resin, completely cuts off extraneous air so that
Sample can keep original form and color for a long time, while avoiding injury of the toxic preservatives to human body.
Existing technology uses the aqueous sample of resin embedding, and the quality problems for being typically easy to occur are：（1）Water in sample
Dividing to be exuded among resin causes transparent resin muddy, and White Flocculus and various impurity, sample table can be produced in resin crystal
Face is atomized, and influences the observation to sample；（2）Sample is shrivelled, and color and metamorphosis are obvious；（3）Long-time storage, sample rots
It is rotten；（4）Resin turns to be yellow with the time, becomes opaque.
The content of the invention
For above technical problem, the invention provides a kind of preparation method of the aqueous zoological specimens of resin embedding, use
Sample crystal water white transparency prepared by the method, the color and form and living specimen of sample is basically identical, in aqueous sample table
Do not hazed in face and crystal or other milky floccules, and sample is placed and does not also change colour, hazes for a long time, crystal does not have
Substantially the phenomenon such as jaundice, explosion occurs.
In this regard, the technical solution adopted in the present invention is：A kind of preparation method of the aqueous zoological specimens of resin embedding, including
Following steps：
Step S1：Preservative treatment, the sample after being handled are carried out to aqueous sample；
Step S2：It will be surface-treated in sample immersion protection resin liquid after the processing, obtain treating specimen embedding；
Wherein, the protection resin liquid includes component A and B component, and the component A is PEPA, and the B component is
Isocyanates；The weight ratio of the component A and B component is 1 ~ 2：1；
Further optimize, the viscosity of the PEPA is 250-600cps, and the viscosity of the isocyanates is
150-500cps。
Step S3：Treat that specimen embedding carries out resin embedding to described.
As a further improvement on the present invention, the weight ratio of the component A and B component is 1 ~ 1.8：1；.
As a further improvement on the present invention, in step S2, the method for the surface treatment is：By the mark after the processing
In this immersion protection resin liquid, vacuumize and be surface-treated, processing time is 1 ~ 10 minute, vacuum is 0.02 ~ 0.1MPa,
Then sample is taken out, allows the resin solidification at least more than 6 hours on its surface, obtain treating specimen embedding.Wherein, the vacuum
More preferably 0.02 ~ 0.05MPa.
As a further improvement on the present invention, the embeding resin liquid includes main glue and curing agent, what the main glue was included
Component and its percentage by weight are：Epoxy resin 80-90%, reactive diluent 0.1-10%, accelerator 0.1-8%, levelling agent 0.1-
1%, ultra-violet absorber 0.1-1%；The curing agent includes at least one of polyetheramine, fatty amine or acid anhydrides.
As a further improvement on the present invention, in step S3, it is to the method for treating that specimen embedding carries out resin embedding：
First embeding resin liquid is poured into mould, will be treated on embeding resin liquid that specimen embedding is put into mould, wherein, it is described to wait to embed
The depth of sample immersion embeding resin liquid is less than the depth of embeding resin liquid in a mold；After embeding resin solidification, continuously add
The embeding resin liquid, makes it cover sample, and solidification obtains the aqueous zoological specimens of resin embedding.
As a further improvement on the present invention, the epoxide equivalent of the epoxy resin is 170-280g/eq, viscous at 25 DEG C
Spend for 5000-25000eps.
As a further improvement on the present invention, the reactive diluent is C12-C14 aliphatic glycidyl ethers, butyl contracting
At least one of water glycerin ether, orthoresol glycidol ether or 1.4 butanediol diglycidyl ethers；The accelerator is nonyl
At least one of phenol or 2 methylimidazoles.
As a further improvement on the present invention, the percentage by weight of the main glue and curing agent is 3 ~ 5：1.
As a further improvement on the present invention, in step S1, after first sample is killed with chloroform, form is arranged, then will
Kept for 2 ~ 6 days in sample immersion impregnating fluid, take out and arrange sample.
During the arrangement form, first the animal of kill is fixed on and arranged on trim panel, it is necessary to solve the first solution plane of plane
It is good to be fixed on progress arrangement form on trim panel again.
Wherein, when arranging sample, if crinite, form is repaired in hair electricity consumption blowing drying.
As a further improvement on the present invention, the component and its percentage by weight that the impregnating fluid is included be：Formaldehyde 3-
10%th, phenol 1-3%, glycerine 3-5%, glacial acetic acid 1-5%, water are supplied.
Compared with prior art, beneficial effects of the present invention are：
First, using technical scheme, the specimen morphology color being embedded in resin is true to nature, and sample surface does not have
The quality problems such as haze.
Second, embeding resin does not haze, turbid phenomenon；Resin transparent degree is high, and being clearly observable sample, each is thin
Section.
3rd, use for a long time, resin does not turn yellow, sample is not corrupt.
Embodiment
The preferably embodiment to the present invention is described in further detail below.
Embodiment 1
A kind of preparation method of resin embedding toad ambroid sample comprises the following steps：
1st, live body toad is killed with chloroform, and jump form is then fixed on trim panel, and size is：7*6.5*2cm,
Intensive aperture is penetrated with syringe needle on water snake epidermis.
2nd, impregnating fluid is prepared：The component and its percentage by weight of impregnating fluid be：Formaldehyde 7.5%, phenol 1%, glycerine 1.5%,
Glacial acetic acid 3% and running water 87%, are well mixed.
3rd, orthopedic toad and trim panel are immersed in impregnating fluid together, soaks 3 days, take out, dry surface water
Point, prepare surface treatment.
4th, the preparation of surface treatment liquid：Surface treatment liquid includes component A and B component, and component A is polyester polyol component,
B component is isocyanate component, and the mass ratio of component A and B component is 1:1, obtain table after component A and B component are well mixed
Face treatment fluid, toad sample is immersed in surface treatment liquid, is put into vacuum tank and starts to vacuumize, vacuum reaches 0.08MPA,
Kept for 1 minute, continue to be evacuated down to 0.04MPA, kept for 1 minute, can both deflated.
5th, the toad after taking-up processing, the processing on the drip-dry surface residual night on filter screen, it is placed on Antisticking and solidifies 6 hours,
Resin embedding process can be entered.
6th, embeding resin is prepared：Embeding resin includes main glue and curing agent, the component and its percentage by weight of main glue formula
For：Standard type epoxy resin NPEL128 87%, butyl glycidyl ether 7.5%, nonyl phenol 5% and ultra-violet absorber uv-196
0.5%, above-mentioned substance is well mixed；The component and its percentage by weight of hardener formula be：Polyetheramine D230 75% and different fluorine
You are ketone diamines IPDA 25%, and both are well mixed.
7th, it is 3 according to the mass ratio of main glue and curing agent by embeding resin:1 well mixed rear vacuum defoamation is embedded
Glue is poured into about 5mm height in 7.5*7.5*2.8cm acrylic moulds by resin glue, vacuum defoamation after being well mixed, will be upper
The toad sample that face is handled well is put into mould, and the bottom of toad is soaked into 5mm resin liquids, is solidified 6 hours, specimen location is
Through fixing, the mixture glue of surface compositions is continuously added, it is concordant with acrylic mould upstream edge until covering sample, Gu
Change 12 hours, just obtain toad specimen embedding.
The resin embedding toad ambroid sample made in the method, color and form and the live body mark of toad sample
This is basically identical, can be clearly observable each organ such as nose, eyes and ear and skin color and gland of sample,
Embeding resin is transparent, and sample surface does not have do not have impurity and floccule in vaporific and muddy thing, resin crystal.The specimen embedding is put
Enter in 50 DEG C of baking ovens 24 hours, be transferred in -18 DEG C of refrigerator-freezers 24 hours, repeatedly for three times, sample does not change colour, hazed, crystal
There is no the quality problems such as turn to be yellow, burst；The specimen embedding is placed on outdoor natural aging 1 month, and sample does not change colour, risen
Mist, crystal does not have the quality problems such as substantially turn to be yellow, burst.
Embodiment 2
A kind of preparation method of resin embedding water snake ambroid sample comprises the following steps：
1st, live body water snake is killed with chloroform, and form of creeping then is fixed on trim panel, and size is：14*5.5*
1.5cm。
2nd, impregnating fluid is prepared：The component and its percentage by weight of impregnating fluid be：Formaldehyde 10%, phenol 3%, glycerine 5%, ice vinegar
Acid 1.5% and running water 80.5%, are well mixed.
3rd, orthopedic water snake and trim panel are immersed in impregnating fluid together, soaks 5 days, take out, dry surface water
Point, prepare surface treatment.
4th, the preparation of surface treatment liquid：Surface treatment liquid includes component A and B component, and component A is polyester polyol component,
B component is isocyanate component, and the mass ratio of component A and B component is 1.5:1, obtained after component A and B component are well mixed
Surface treatment liquid, water snake sample is immersed in surface treatment liquid, is put into vacuum tank and starts to vacuumize, vacuum reaches
0.08MPA, is kept for 3 minutes, is continued to be evacuated down to 0.01MPA, is kept for 3 minutes, can both deflated.
5th, the water snake after taking-up processing, the processing on the drip-dry surface residual night on filter screen, it is placed on Antisticking and solidifies 6 hours,
Resin embedding process can be entered.
6th, embeding resin is prepared：Embeding resin includes main glue and curing agent, the component and its percentage by weight of main glue formula
For：Standard type epoxy resin NPEL128 89.5%, C12-C14 aliphatic glycidyl ethers AGE 9.5%, the and of 2-methylimidazole 1%
Ultra-violet absorber uv-196 0.5%, above-mentioned substance is well mixed；The component and its percentage by weight of curing agent be：Polyethers
Amine T403 60%, polyetheramine D400 30% and m-xylene diamine 10%, three are well mixed.
7th, it is 4.5 according to the mass ratio of main glue and curing agent by embeding resin:1 well mixed rear vacuum defoamation is wrapped
Resin glue is buried, then embeding resin glue is poured into about 5mm height in 160*7.5*2.8cm acrylic moulds, will be handled above
Good water snake sample is put into mould, and the bottom of water snake is soaked into 5mm resin liquids, is solidified 6 hours, specimen location has been fixed
It is good, the embeding resin glue of surface compositions is continuously added, concordant with acrylic mould upstream edge until covering sample, solidification
12 hours, just obtain water snake specimen embedding.
The resin embedding water snake ambroid sample made in the method, crystal water white transparency, the color of water snake sample
It is basically identical with living specimen with form, do not hazed in aqueous sample surface and crystal or other milky floccules.
The specimen embedding is put into 50 DEG C of baking ovens 24 hours, is being transferred in -18 DEG C of refrigerator-freezers 24 hours, repeatedly for three times, sample does not become
Color, haze, crystal does not have the quality problems such as turn to be yellow, burst；The specimen embedding is placed on outdoor natural aging 1 month, sample
Do not change colour, haze, outward appearance is not substantially turned to be yellow, the quality problems such as no explosion occur.
Above content is to combine specific preferred embodiment further description made for the present invention, it is impossible to assert
The specific implementation of the present invention is confined to these explanations.For general technical staff of the technical field of the invention,
On the premise of not departing from present inventive concept, some simple deduction or replace can also be made, should all be considered as belonging to the present invention's
Protection domain.
Claims (7)
1. a kind of preparation method of the aqueous zoological specimens of resin embedding, it is characterised in that comprise the following steps：
Step S1：Preservative treatment, the sample after being handled are carried out to aqueous zoological specimens；
Step S2：It will be surface-treated in sample immersion protection resin liquid after the processing, obtain treating specimen embedding；
Wherein, the protection resin liquid includes component A and B component, and the component A is PEPA, and the B component is isocyanide
Acid esters；The weight ratio of the component A and B component is 1 ~ 2：1；The method of the surface treatment is：By the sample after the processing
In immersion protection resin liquid, vacuumize and be surface-treated, processing time is 1 ~ 10 minute, vacuum is 0.02 ~ 0.1MPa, so
Sample is taken out afterwards, the resin solidification at least more than 6 hours on its surface is allowed, obtains treating specimen embedding；
Step S3：Treat that specimen embedding carries out resin embedding to described, first embeding resin liquid is poured into mould, specimen embedding will be treated
It is put on the embeding resin liquid in mould, wherein, the depth for treating specimen embedding immersion embeding resin liquid is less than embeding resin
The depth of liquid in a mold；After embeding resin solidification, the embeding resin liquid is continuously added, it was covered sample, solidification is obtained
The aqueous zoological specimens of resin embedding；The embeding resin liquid includes main glue and curing agent, component that the main glue is included and its again
Measuring percentage is：Epoxy resin 80-90%, reactive diluent 0.1-10%, accelerator 0.1-8%, levelling agent 0.1-1%, ultraviolet
Absorbent 0.1-1%；The curing agent includes at least one of polyetheramine, fatty amine or acid anhydrides.
2. the preparation method of the aqueous zoological specimens of resin embedding according to claim 1, it is characterised in that：The component A
Weight ratio with B component is 1~1.8：1.
3. the preparation method of the aqueous zoological specimens of resin embedding according to claim 1, it is characterised in that：The asphalt mixtures modified by epoxy resin
The epoxide equivalent of fat is that the viscosity at 170-280g/eq, 25 DEG C is 5000-25000eps.
4. the preparation method of the aqueous zoological specimens of resin embedding according to claim 1, it is characterised in that：The activity is dilute
Agent is released for C12-C14 aliphatic glycidyl ethers, butyl glycidyl ether, orthoresol glycidol ether or the contracting of 1.4- butanediols two
At least one of water glycerin ether；The accelerator is at least one of nonyl phenol or 2-methylimidazole.
5. the preparation method of the aqueous zoological specimens of resin embedding according to claim 1, it is characterised in that：The main glue with
The percentage by weight of curing agent is 3 ~ 5：1.
6. the preparation method of the aqueous zoological specimens of resin embedding according to claim 1, it is characterised in that：In step S1,
After first sample is killed with chloroform, form is arranged, then sample is immersed in impregnating fluid and kept for 2 ~ 6 days, takes out and arranges sample.
7. the preparation method of the aqueous zoological specimens of resin embedding according to claim 6, it is characterised in that：The impregnating fluid
Comprising component and its percentage by weight be：Formaldehyde 3-10%, phenol 1-3%, glycerine 3-5%, glacial acetic acid 1-5%, water are supplied.
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| CN111213632B (en) * | 2019-11-19 | 2022-03-01 | 长春中医药大学 | Method for preparing animal medicine epoxy resin specimen |
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