CN106818703A - A kind of small fishes primary colors solid phase Slide processing - Google Patents
A kind of small fishes primary colors solid phase Slide processing Download PDFInfo
- Publication number
- CN106818703A CN106818703A CN201710191816.0A CN201710191816A CN106818703A CN 106818703 A CN106818703 A CN 106818703A CN 201710191816 A CN201710191816 A CN 201710191816A CN 106818703 A CN106818703 A CN 106818703A
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- Prior art keywords
- fish
- solid phase
- primary colors
- small fishes
- slide processing
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a kind of small fishes primary colors solid phase Slide processing, including 1) anesthesia, 2) it is fixed, 3) buffer, 4) seal step up for safekeeping.Present invention is especially suited for the preservation of fish bright in luster, color is identical or approximate when after treatment with life, has reached the purpose of vivid displaying.Tested by 2 years, the present invention can not only preserve sample body, moreover it is possible to preserve the original color of fish, be suitable to small-sized fancy fishes, particularly preferable to black, white, red colour system fish preservation.Made sample is easy to carry and observes, while having art appreciation and reserve value.
Description
Technical field
The invention belongs to zoological specimens preparation field, and in particular to a kind of small fishes primary colors solid phase Slide processing.
Background technology
Fishes snd preparation method typically has humid preparation, skeleton specimen and skinned-specimen three types, and skeleton specimen is not
Beneficial to display configuration structure, skinned-specimen is suitable to the sample that volume is big, scale is difficult for drop-off, therefore comparatively soak mark
The comparing of this application is wide.Current fish humid preparation is typically fixed by formalin solution and preserved, and dipped into formalin method is not
It is in place of foot：1st, because formaldehyde can constantly volatilize, formalin solution need to be added to be supplemented every some times.2nd, formaldehyde is waved
Contaminated air after hair, also there is injury effect to human body, inadequate health and safety.3rd, tissue elasticity is poor, and muscle is pale stiff.Especially
It is easily to cause sample color loss, is unfavorable for the preservation of fish bright in luster, is very different when after treatment with life,
The purpose of vivid displaying can not be reached.4th, carry inconvenient.
On the other hand, although dry preserved specimen has the characteristics of showing fish vividly, for small fishes shape
The method not only operating difficulties that the making of state sample, such as side filling side seam are closed, and it is difficult to show the variform of fish.
The content of the invention
In view of this, the first object of the present invention is to provide a kind of small fishes primary colors solid phase Slide processing, bag
Include following steps：
1) anaesthetize：Anesthetic to fish is added to anaesthetize in water body where toward the small fishes for needing to make sample；
2) it is fixed：The fish for anaesthetizing motionless is pulled out and is put into fixer, fish body of setting right stands and fixes；
3) buffer：Fixer is outwelled, adds buffer solution to embathe；
4) seal up for safekeeping：The small fish sample that will have been embathed is pulled out from buffer solution, and fish body surface extraction raffinate is gently blotted with absorbent cloth
Afterwards, be put into sealing mould up for safekeeping, toward mould in fill it up with colourless nail polish, adjust fish body position with dissecting needle, stand to solidifying, the phase
Between nail polish can be added to supplement volatile fraction.
Preferably, in small fishes primary colors solid phase Slide processing of the present invention, the step 1) in be to enter
Before row is fixed, fish body is anaesthetized.
Preferably, in small fishes primary colors solid phase Slide processing of the present invention, the step 2) in fixer
Formula be formaldehyde 5-10ml, absolute ethyl alcohol 30ml, acetic acid 2-5ml, sodium acetate 1-3g, plus distilled water is to 100ml.
Preferably, in small fishes primary colors solid phase Slide processing of the present invention, the step 2) in it is fixed
Time is 12 hours~48 hours.
Preferably, in small fishes primary colors solid phase Slide processing of the present invention, the step 3) in buffer solution
Formula be formaldehyde 2-5ml, sodium acetate 1-3g, glycerine 10-20mlml, eugenol 0.1ml, plus distilled water is to 100ml.
Preferably, in small fishes primary colors solid phase Slide processing of the present invention, the step 4) in colourless finger
Nail polish is commercially available General nail oil.
Present invention also offers small fishes primary colors solid phase sample prepared by the above method, wherein the small fishes is big
Small is total length<The fish of below 5cm or the juvenile fish of other fish same sizes.
From the foregoing, it will be observed that the present invention at least has the advantage that：
1) inventors herein have recognized that, by toward being slowly added to fiber crops in water body where the small fishes for needing to make sample
Liquor-saturated dose, to fish anesthesia, both can to greatest extent retain the freshness before small fishes sample disposal, and small-sized fish can be fixed again
Class natural lifestyle before death, makes the specimen morphology after making more lively, true to nature, closer to the original form of fish；
2) present invention is especially suited for the preservation of fish bright in luster, color is identical or approximate when after treatment with life, reaches
The purpose of vivid displaying.
3) tested by 2 years, the present invention can not only preserve sample body, moreover it is possible to preserve the original color of fish, be suitable to small
Type fancy fishes, it is particularly preferable to black, white, red colour system fish preservation.Made sample is easy to carry and observes, while having
Art appreciation and reserve value.
Brief description of the drawings
Fig. 1 is the Poecilia primary colors solid phase sample figure in one embodiment of the present of invention.
Specific embodiment
Further technical scheme is illustrated below by way of specific embodiment, it should be understood that be only below this hair
Bright exemplary illustration, is not intended to limit the invention scope of the claims.
Embodiment 1
1) anaesthetize：By 3cm long or so, the Poecilia for being fabricated to sample is needed to reap in beaker, to being slowly added dropwise in beaker
Eugenol to fish is anaesthetized；
2) it is fixed：The fish for anaesthetizing motionless is pulled out and is put into fixer, align fish body, stand fixed 24h.Fixer is by following
Method is prepared：Formaldehyde 5ml, absolute ethyl alcohol 30ml, acetic acid 2ml, sodium acetate 2g, plus distilled water is to 100ml.
3) buffer：Fixer is outwelled, adds buffer solution to embathe 24h.The compound method of buffer solution is formaldehyde 2ml, acetic acid
Sodium 2g, glycerine 10ml, eugenol 0.1ml, plus distilled water is to 100ml.
4) seal up for safekeeping：The small fish sample that will have been embathed is pulled out from buffer solution, and fish body surface extraction raffinate is gently blotted with absorbent cloth
Afterwards, it is put into sealing mould up for safekeeping as shown in Figure 1, and is put into pasture and water segment pasture and water as decoration, then toward fills it up with colourless finger in mould
Nail polish, fish body position is adjusted with dissecting needle, is stood to solidifying, and period adds nail polish to supplement volatile fraction.
Make result as shown in Figure 1.As shown in figure 1, the Poecilia solid phase sample for making by this method was by 2 years
The preservation of time remains in that initial color, and preservation effect is preferable.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (6)
1. a kind of small fishes primary colors solid phase Slide processing, comprises the following steps：
1) anaesthetize：Anesthetic to fish is added to anaesthetize in water body where toward the small fishes for needing to make sample；
2) it is fixed：The fish for anaesthetizing motionless is pulled out and is put into fixer, fish body of setting right stands and fixes；
3) buffer：Fixer is outwelled, adds buffer solution to embathe；
4) seal up for safekeeping：The small fish sample that will have been embathed is pulled out from buffer solution, after fish body surface extraction raffinate is gently blotted with absorbent cloth, is put
In entering to seal up for safekeeping mould, toward mould in fill it up with colourless nail polish, adjust fish body position with dissecting needle, stand to solidifying, period can
Nail polish is added to supplement volatile fraction.
2. small fishes primary colors solid phase Slide processing according to claim 1, it is characterised in that be fixed it
Before, fish body is anaesthetized.
3. small fishes primary colors solid phase Slide processing according to claim 1, it is characterised in that the step 2) in
The formula of fixer is formaldehyde 5-10ml, absolute ethyl alcohol 30ml, acetic acid 2-5ml, sodium acetate 1-3g, plus distilled water is to 100ml.
4. small fishes primary colors solid phase Slide processing according to claim 1, it is characterised in that the step 3) in
The formula of buffer solution is formaldehyde 2-5ml, sodium acetate 1-3g, glycerine 10-20ml, eugenol 0.1ml, plus distilled water is to 100ml.
5. small fishes primary colors solid phase Slide processing according to claim 1, it is characterised in that the step 4) in
Colourless nail polish is commercially available General nail oil.
6. the small fishes primary colors solid phase sample for being prepared according to Claims 1 to 5 any one methods described, it is characterised in that
The size of the small fishes is total length<The fish of below 5cm or the juvenile fish of other fish same sizes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710191816.0A CN106818703A (en) | 2017-03-28 | 2017-03-28 | A kind of small fishes primary colors solid phase Slide processing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710191816.0A CN106818703A (en) | 2017-03-28 | 2017-03-28 | A kind of small fishes primary colors solid phase Slide processing |
Publications (1)
| Publication Number | Publication Date |
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| CN106818703A true CN106818703A (en) | 2017-06-13 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN201710191816.0A Pending CN106818703A (en) | 2017-03-28 | 2017-03-28 | A kind of small fishes primary colors solid phase Slide processing |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106818704A (en) * | 2017-03-28 | 2017-06-13 | 青岛农业大学 | A kind of small fishes primary colors humid preparation preparation method |
| CN110586562A (en) * | 2019-09-17 | 2019-12-20 | 浙江海洋大学 | Method for identifying sebastes schlegeli hilgendorf population |
| CN113180033A (en) * | 2021-04-28 | 2021-07-30 | 黄倞朗 | Preparation method of small biological specimen |
Citations (5)
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| JPS63284101A (en) * | 1987-05-14 | 1988-11-21 | Akio Imuro | Production of specimen of animal body or mushrooms |
| CN101305710A (en) * | 2008-06-18 | 2008-11-19 | 陈庆明 | Corpus antiseptic and its preparation method |
| CN103583509A (en) * | 2013-11-27 | 2014-02-19 | 青岛海洋科技馆 | Making method of gel specimen |
| CN105010302A (en) * | 2015-07-08 | 2015-11-04 | 南宁千年工艺有限公司 | Method for preparing resin-embedded water-containing animal specimen |
| CN105248410A (en) * | 2015-11-17 | 2016-01-20 | 广东海洋大学 | Preservation method for biological specimen |
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2017
- 2017-03-28 CN CN201710191816.0A patent/CN106818703A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63284101A (en) * | 1987-05-14 | 1988-11-21 | Akio Imuro | Production of specimen of animal body or mushrooms |
| CN101305710A (en) * | 2008-06-18 | 2008-11-19 | 陈庆明 | Corpus antiseptic and its preparation method |
| CN103583509A (en) * | 2013-11-27 | 2014-02-19 | 青岛海洋科技馆 | Making method of gel specimen |
| CN105010302A (en) * | 2015-07-08 | 2015-11-04 | 南宁千年工艺有限公司 | Method for preparing resin-embedded water-containing animal specimen |
| CN105248410A (en) * | 2015-11-17 | 2016-01-20 | 广东海洋大学 | Preservation method for biological specimen |
Non-Patent Citations (4)
| Title |
|---|
| 葛海龙 等: ""几种观赏鱼原色标本制作方法"", 《南方农业》 * |
| 詹益兴 等: "《化工小商品生产法》", 31 March 1993, 湖南科学技术出版社 * |
| 诸君汉 等: "《实验室实用手册》", 31 October 1994, 机械工业出版社 * |
| 趙尔炎 等: ""保存原色的小鱼标本的制作"", 《生物学通报》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106818704A (en) * | 2017-03-28 | 2017-06-13 | 青岛农业大学 | A kind of small fishes primary colors humid preparation preparation method |
| CN110586562A (en) * | 2019-09-17 | 2019-12-20 | 浙江海洋大学 | Method for identifying sebastes schlegeli hilgendorf population |
| CN110586562B (en) * | 2019-09-17 | 2022-01-11 | 浙江海洋大学 | Method for identifying sebastes schlegeli hilgendorf population |
| CN113180033A (en) * | 2021-04-28 | 2021-07-30 | 黄倞朗 | Preparation method of small biological specimen |
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Application publication date: 20170613 |