JPS63284101A - Production of specimen of animal body or mushrooms - Google Patents

Abstract

PURPOSE:To obtain the titled specimen useful for the naked eye, retaining the original form of animal body, etc., free from stain, by dehydrating the animal body, etc., permeating a transparent resin to the surface layer part of the animal body, etc., and curing the resin by ultraviolet rays before the resin is diffused into the interior. CONSTITUTION:First an animal body such as fishes, the reptiles, mollusks or mushrooms fixed with formalin, etc., is freeze dried or dehydrated by organic solvent dehydrating method and a transparent resin such as acrylic resin or polyester resin is permeated into the surface layer part of the animal body. Before the resin is diffused into the interior, preferably the resin to be permeated is mixed with a sensitizer, the resin is cured by ultraviolet rays or electron rays, the transparent resin is permeated into the animal body without obstacles, the resin is rapidly cured and a hard coating film of the transparent resin is made on the surface layer part to give the aimed specimen. The specimen is a good specimen for the naked eye when the specimen is directly stored in a glass container or when the transparent resin is subjected to embedding processing.

Description

DETAILED DESCRIPTION OF THE INVENTION (Industrial Application Field) The present invention relates to a method for producing macroscopic specimens of animals and fungi that retain their original shapes.

(Prior art) In the past, specimens of animals and mushrooms for the naked eye were usually manufactured as specimens embedded in transparent resins such as acrylic and polyester from the viewpoint of shape retention and preservation. The first order is known.

(1) Surface drying method: A method in which warm dry air is applied to the surface of the sample for a short period of time to dry only one surface, and the sample is then immediately embedded).
If the contents have been fixed in advance with formalin,
It is possible to create specimens that can be stored for a certain period of time.

However, the result is extremely unstable, as air bubbles are formed due to internal moisture, and the resin becomes white and lumpy. Furthermore, due to the high internal moisture content, it is not suitable for long-term storage.

(2) Sugar solution immersion method This is a method in which the sample is impregnated with about 50 to 80 drops of sugar solution and then embedded, and the above method has a good shelf life.

There is less worry about the resin getting wet compared to the above.

However, when impregnated with sugar solution, the object inevitably shrinks. This becomes more serious as the sugar concentration increases. On the other hand, Koshi is good for preservation and resin.

Since it will not improve unless the m concentration is increased, many of them are unsuitable from the standpoint of preserving the M form.

(3) After freeze-drying or organic solvent substitution dehydration, a transparent resin top layer is impregnated, then a resin prepolymer is impregnated, a curing agent is added thereto, and the method is embedded in the same resin. Although specimens with good preservability can be obtained, the disadvantage is that the larger the specimen, the more the specimen shrinks during the curing process.

Furthermore, as the sample size increases, the amount of embedding resin inevitably increases, so a long-time curing method must be used to suppress the heat of the curing reaction. If you do not do this, the sample will change color due to the heat of reaction.9. In severe cases, the work may crack and foam. During curing, over time the low viscosity prepolymer inside the sample leaches into the increasingly viscous polymer outside. Take the example of acrylic, a large piece of work to prevent this harm.

The limit is to harden a few millimeters within 20 to 50 minutes.

Another disadvantage of this method is that the resin monomer has strong properties as an oil-soluble solvent, so samples containing fat-soluble dyes tend to lose their color and become translucent. be. Examples of animals that lose their color include Oikawa, Fuefukatsuko, and Tamagotake mushrooms, which have a nuptial color, while examples of transparency include animal brains, Koreitake mushrooms, sweetfish, and wood ear mushrooms.

Depending on the conventional method described above, the original shape and primary color of specimens such as animals and mushrooms can be maintained using acrylic.

It was difficult to embed it in transparent resin such as polyester. Therefore, as a method to solve the problems of the above-mentioned conventional method, the present inventors proposed a method for solving the problems of the above-mentioned conventional method. Firstly, a mixture of polyester resin and/or acrylic resin and a curing agent is infiltrated into the surface layer of the sample and rapidly gelled. After cooling, a transparent potting resin is obtained. He has already invented a method for producing transparent resin-embedded specimens characterized by embedding them in
issue).

(Problems to be Solved by the Invention) According to the above-mentioned Japanese Patent Publication No. 57-57441, the sample does not undergo whitening or other discoloration, and does not become transparent at t7t.
Furthermore, even samples with a reduced number of people do not undergo deterioration due to curing heat and are good as embedded specimens for the naked eye. If the amount is large enough, it will harden even at room temperature, so it will harden during the process of penetrating into the surface layer of the animal body, and will begin to harden during the process of penetrating the surface layer of the animal body. If the amount of hardening agent is reduced and the hardening agent penetrates into the surface layer of the animal's body before hardening begins, heating will accelerate hardening.
When heated, ingredients present inside the animal body leach out,
The specimens are neat and dry.

It was also found that there was a problem in that the curing was not carried out sufficiently, resulting in a sticky state.

(Means for Solving the Problems) As a result of intensive studies to solve all of the above-mentioned problems, the inventors of the present invention have developed a vertical coating of transparent resin of a certain thickness on the surface layer of animal bodies or mushrooms. From the viewpoint that it is a good idea to make such a material, the present invention has been completed as a method that can do it most appropriately. That is, in one aspect of the present invention, after dehydrating an animal body or mushrooms, transparent wood is impregnated into the surface layer of the animal body or mushrooms, and before it is diffused into the interior.

This is a method for producing specimens of animals and mushrooms, which is characterized by curing resin with ultraviolet rays or electron beams.

In the present invention, first, the animal bodies or mushrooms are fixed in fresh or formalin, and the freeze-dried mushrooms are dehydrated by an organic solvent dehydration method (the solvent is cold alcohol, cold acetone, etc.). If dehydration is not performed, specimens will rot and have poor preservation.

Next, a transparent resin such as acrylic or polyester is soaked into the surface layer of the animal or mushroom, and before being diffused inside, the resin is cured with ultraviolet rays or electron beams to form a hard coating of transparent resin on the surface layer. However, this ultraviolet curing or electron beam curing can be done by applying ultraviolet or electron beam curing methods for general synthetic resins.
s Therefore, in the case of ultraviolet curing, it is preferable to incorporate a sensitizer into the resin that is impregnated into the surface layer of the animal body or mushroom. However, since electron beams have much higher energy (10I times the brilliance) than visible to ultraviolet light, electrons1ll1fI! In this case, it is not necessary to contain a sensitizer.

If this ultraviolet curing or electron beam curing is performed.

There is no I/C barrier to penetration of the transparent resin into animals or mushrooms, and the resin can be rapidly cured before diffusing inside.

Before the transparent resin is infiltrated into the surface layer of the animal body or mushrooms, that is, after the animal body or mushrooms are dehydrated, a white fibrous substance or a transparent resin having a different refractive index from the surface layer resin is infiltrated into the interior. It is preferable to replace the water with moisture and leave it in a friendly state, but of course, good results can be obtained even if it is left dehydrated.

Specimens of animals such as fish, reptiles, molluscs, or mushrooms obtained in this way can be kept in glass containers as they are, but the temperature may change when embedding them in transparent resin. However, the meat 11, which is good in quality, is used as a specimen.

(Effects of the Invention) According to the present invention, the transparent resin can penetrate into the animal body or mushrooms without any hindrance, and the resin can be rapidly hardened, so that the transparent resin can penetrate into the surface layer of the animal body or mushrooms. It is possible to accurately form the resin coating X with an appropriate thickness. Moreover,
Since heating is not required as in thermosetting, components present inside the animal body or mushrooms do not leach out, resulting in a clean collar.

(Example) below,! This will be explained by giving an example.

Example 1 Resin-embedded specimen of crucian carp A carp was immersed in 10 times formalin water to fix it in formalin, then dehydrated by immersing it in -150 ethanol, and then immersed in acrylic monomer to completely replace it. Add an ultraviolet sensitizer to a mixture of polyester prepolymer and acrylic monomer, immerse the mixture in a container, and when the mixture reaches at least the shallow layer, take it out and irradiate it with ultraviolet rays to harden the gold tooth resin. After that, it is embedded in acrylic resin.

Example 2 Resin-embedded specimen of tuna kajika Tuna kajika was immersed and fixed in 10 times formalin water, and then immersed in propylene glycol for 50 h.

709G, dehydrated by sequentially immersing in 100 solutions 1
Next, a UV sensitizer was added to the acrylic prepolymer.
If the key is at least shallow, it is taken out. After being irradiated with ultraviolet light to harden the gold resin, it is embedded in acrylic resin.

Example 5 Visceral specimens Brain sections were soaked in tertiary butyl alcohol with 5096.
Dehydrated by sequentially immersing in solutions of 70 and 100.1 Next, immersing in a solution of butyl alcohol sol of cetyl alcohol and a solution of pure cetyl alcohol.1 Completely replacing the cetyl alcohol with cetyl alcohol, and then cleaning the surface layer with a solvent. After washing, this is immersed in a polyester prepolymer with an ultraviolet sensitizer added, and if the key is matched at least in the shallow layer, it is taken out.It is irradiated with ultraviolet AT to harden the gold tooth resin.

This can be observed by stacking each section and separating them.

Example 4 Embedded specimen of Shiikuke Shiitake mushrooms were soaked in 77 tons of solution and dehydrated.

Next, the specimen is immersed in an acrylic monomer, and an embedded specimen is prepared in the same manner as in Example 1.

Example 5 Embedding specimen of whitefish After anesthetizing a whitefish, it is immersed in -15C pyridine. Once the water has been completely replaced, return to room temperature, add a small amount of polyester prepolymer to pyridine and add to the liquid, then gradually increase the amount of prepolymer until the polymer content reaches 80-. After UV curing in the same manner as in Example 1.

Embed in polyester resin.

Example 6 Embedded specimen of potato After a living body is anesthetized, it is frozen and then placed in a methyl seron lube cooled to below -4t:'. After the dehydration and displacement are completed, the acrylic prepolymer is added little by little, and an embedded specimen is then prepared in the same manner as in Example 1.

Claims (1)

[Claims] After dehydrating the animal body or mushrooms, a transparent resin is impregnated into the surface layer of the animal body or mushrooms, and the resin is cured with ultraviolet rays or electron beams before being diffused into the interior. Mushroom specimen production method.