KR102495871B1 - Composition for preservation treatment of plant and treating method using it - Google Patents

Abstract

Disclosed in the present invention are: a plant reservation treatment composition capable of maintaining the color and shape of plants by inhibiting drying and decay due to plant cell transformation; and a plant treatment method using the same. The plant reservation treatment composition of the present invention contains an antibacterial agent, an internal treatment agent, a surface treatment agent, and a filling treatment agent.

Description

Composition for preservation treatment of plant and treating method using it {Composition for preservation treatment of plant and treating method using it}

The present invention relates to a composition for preserving and treating plants and a method for treating plants using the same.

Plants change their shape or color depending on the season, have limited habitats, and can become extinct due to natural disasters or the environment. Since it is practically impossible to store living plants for a long time, these plants are produced in the form of specimens.

A plant specimen is a record that reflects the most realistic temporal and spatial conditions of a plant species, as it preserves the plant as it is without deformation of its form.

In the production of existing plant specimens, it is common to dry and press plants, but it is difficult to store them for more than 1000 days, such as being crushed or discolored after a certain period of time.

As an alternative to this, there is a method of putting plants in resin and curing it. Specifically, a method of treating plants with preservatives and preservatives and then putting them in a liquid resin and curing is used.

The method using resin has the advantage of increasing the storage days and maintaining the shape of the plant, but there is a problem in that the original color of the plant cannot be maintained due to discoloration after the preservative treatment. In addition, when living plants are used, it is difficult to properly mix them in the liquid resin, and after curing, the plants are dried and deformed. Therefore, when dried plants are used, pressed plants are easily crushed and difficult to store, and when cured after being put into the resin, the original color does not come out.

In order to solve this problem, a method using a resin after treating living plants with a separate preservative (eg, formalin) has been proposed. However, preservatives induce transformation of plant cells, making it difficult to maintain plant-specific color and shape, causing drying and decay, and drying after treatment with preservatives, which requires at least one day of work.

Prior Patent Literatures 1 and 2 suggest that by treating plants using polymers such as polyethylene glycol or polypropylene glycol, it is possible to extend the storage period and preserve texture and the like. However, this method has a problem in that a processing period of at least 3 to 5 days is required.

KR Publication No. 10-2005-0037008 (published on April 21, 2005) KR Registered Patent No. 10-0916984 (Announced on September 14, 2009)

The present applicant has conducted research to preserve living plants, but to solve problems caused by cell transformation, such as discoloration, crumbling, and decay, and designed a composition for preserving plants with a specific composition.

As a result of the treatment using the composition for preserving plants, it was confirmed that the treatment can be performed within a short time within 1 day, and that the form of the plant can be maintained without cell deformation and can be permanently preserved when produced as a plant specimen.

The surface treatment agent for plant preservation of the present invention includes at least one polar polymer selected from the group consisting of polyvinylpyrrolidone, polyvinylbutyral, polyacrylic acid, and cellulose; and one solvent selected from water, ethanol, isopropyl alcohol, or a mixed solvent thereof.

The composition for preserving plants of the present invention includes an antibacterial agent, an internal treatment agent, a surface treatment agent, and a filling treatment agent.

The surface treatment agent is at least one polar polymer selected from the group consisting of polyvinylpyrrolidone, polyvinylbutyral, polyacrylic acid, and cellulose; And, one solvent selected from water, ethanol, isopropyl alcohol, or a mixed solvent thereof; includes.

The antimicrobial agent is an aqueous solution of NaOCl.

The internal treatment agent is one organic solvent selected from acetone, ethanol, isopropanol or a mixture thereof, one organic acid selected from the group consisting of formic acid, acetic acid, and lactic acid, and formaldehyde, paraformaldehyde, or mixtures thereof It includes one selected from one selected aldehyde-based compound.

The filling treatment agent includes at least one filler selected from the group consisting of silica and silane and a solvent.

The silane is at least one selected from the group consisting of alkyl silane, alkoxy silane, vinyl silane, amino silane, acrylate silane, epoxy silane, isocyanate silane, and hydrolysis silane.

Plant preservation treatment method of the present invention of one embodiment includes the step of immersing the plant in the plant preservation treatment composition and then drying.

In another embodiment of the method for preserving plants of the present invention, plants are treated with the composition for preserving plants in multiple steps.

Specifically, it includes drying the plant after treatment with an antibacterial agent, drying after treatment with an internal treatment agent, drying after treatment with a surface treatment agent, and drying after treatment with a filling treatment agent.

In addition, the plant specimen of the present invention is prepared by immersing a plant preserved by the above method in a resin and then curing it.

In addition, in the plant specimen of the present invention, the plant preserved by the above method is immersed in a preservation solution and then sealed.

The plant conservation treatment method according to the present invention maintains the shape of the plant without cell transformation, enabling permanent preservation when produced as a plant specimen.

1 (a) to (c) are photographs of plant specimens of plantain leaves treated in Example 1, Comparative Example 3 and Comparative Example 5.

The present invention discloses a treatment method capable of maintaining the shape of a living plant without problems such as discoloration, crumbling, and decay due to cell transformation, a composition used therein, and a method of manufacturing a plant specimen using the same.

In this specification, plants can be all plants constituting the plant kingdom, and bryophytes, angiosperms, gymnosperms, vascular plants, perennials, medicinal plants, aquatic plants, carnivorous plants, alpine plants, salt plants, organ-transforming plants, etc. is possible At this time, the plant for which a plant sample is available may be the whole plant or a part thereof, and the part includes all flowers, fruits, leaves, roots, stems, and the like.

In this specification, a live plant means a plant immediately after collection that has not been chemically treated, pressed and/or dried.

Surface treatment agent for plant conservation treatment

When a non-dried plant, that is, a living plant is sampled using resin, cell transformation occurs due to a treatment agent used, making it difficult to maintain the plant's own color and shape. In addition, while the plant is hydrophilic, the curing resin is hydrophobic, and after curing, separation between the plant and the curing resin occurs, and additional decay occurs due to this separation, resulting in deformation of the plant's own color and shape.

The surface treatment agent of the present invention increases adhesion with the resin used in manufacturing plant specimens to inhibit cell transformation, thereby preventing peeling and decay of plant specimens.

Specifically, the surface treatment agent of the present invention is selected from at least one polar polymer selected from the group consisting of polyvinylpyrrolidone, polyvinylbutyral, polyacrylic acid, and cellulose, water, ethanol, isopropyl alcohol, or a mixed solvent thereof. Contains one selected solvent.

Polar polymers such as polyvinylpyrrolidone, polyvinylbutyral, polyacrylic acid, and cellulose have excellent adhesion to hydrophobic materials and also excellent adhesion to hydrophilic materials. As a result, excellent adhesion between hydrophilic plants and hydrophobic resin is ensured, and peeling with the resin is prevented when the plant surface is treated. Accordingly, since no gap is generated between the resin and the plant, problems such as additional decay and drying do not occur, so that the color and shape of the plant can be maintained as it is.

The polar polymer is used in a solution state for treatment, and alcohol used as a solvent or a mixed solvent of water and alcohol is used as a solvent. The alcohol is ethanol or isopropyl alcohol.

The polar polymer in the surface treatment agent is used in an amount of 20 to 80% by weight, and the solvent is used in an amount of 20 to 80% by weight. If the content of the polar polymer is too low, the effect of using the surface treatment agent is insignificant, and if it is too excessive, it may cause deformation of plant cells.

According to one example, the surface treatment agent includes 20 to 80% by weight of polyvinylpyrrolidone, 10 to 40% by weight of ethanol, and 10 to 40% by weight of isopropyl alcohol so as to satisfy 100% by weight of the total.

According to another example, the surface treatment agent includes 20 to 80% by weight of polyvinyl butyral, 10 to 40% by weight of ethanol, and 10 to 40% by weight of isopropyl alcohol so as to satisfy 100% by weight in total.

According to another example, the surface treatment agent includes 20 to 80% by weight of polyacrylic acid, 9 to 30% by weight of ethanol, 9 to 30% by weight of isopropyl alcohol, and 2 to 20% by weight of water so as to satisfy a total of 100% by weight. .

According to another example, the surface treatment agent includes 20 to 80% by weight of cellulose, 9 to 30% by weight of ethanol, 9 to 30% by weight of isopropyl alcohol, and 2 to 20% by weight of water so as to satisfy a total of 100% by weight.

Composition for Preservation of Plants

The composition for preserving plants of the present invention includes an antibacterial agent, an internal treatment agent, and a filling treatment agent together with the surface treatment agent as described above.

Antibacterial agents use the oxidation phenomenon caused by oxygen supply in the generator to kill bacteria and viruses present on the plant surface. As the antibacterial agent, any material that creates nascent oxygen can be used, preferably using a 0.5 to 5% by weight aqueous solution of NaOCl, preferably a 1% by weight aqueous solution, or a commercially available lacquer may be purchased and diluted. If not treated with the antimicrobial agent, plant decay may proceed after plant specimen production.

As the internal treatment agent penetrates the plant and induces a chemical reaction inside the plant, the plant cell remains dead but maintains the cell structure so that the color and shape of the plant can be maintained.

The internal treatment agent may be at least one selected from the group consisting of organic solvents, organic acids, and aldehyde-based compounds.

The organic solvent is one selected from acetone, ethanol, isopropanol, and mixed solvents thereof, the organic acid is one selected from the group consisting of formic acid, acetic acid, lactic acid, and mixtures thereof, and the aldehyde-based compound is formaldehyde, para It is one selected from formaldehyde or a mixture thereof. According to one embodiment, the internal treatment agent is used by mixing acetone, ethanol and acetic acid.

The filling treatment agent is used to fill the empty space on the surface of the plant due to the treatment of the internal treatment agent and the surface treatment agent. The filling treatment agent includes at least one filler selected from the group consisting of silica and silane and a solvent. The silane is at least one selected from the group consisting of alkyl silane, alkoxy silane, vinyl silane, amino silane, acrylate silane, epoxy silane, isocyanate silane, and hydrolysis silane.

In this case, the filling treatment agent may be a dispersion containing 20 to 80% by weight of the filler and 20 to 80% by weight of the solvent. In particular, if the refractive index of the plant differs from the filler, it may appear hazy after preparing the plant specimen, and a filler similar to the refractive index of general organic matter (1.45-1.53) is used. The solvent may be one selected from water, ethanol, isopropanol, and a mixed solvent thereof. In this case, the silica may be nano silica having an average particle diameter of 0.1 to 1000 nm, 0.5 to 900 nm, 1.0 to 800 nm, and 5 to 500 nm.

The composition for preserving plants of the present invention contains 0.01 to 20% by weight of an antibacterial agent, 0.1 to 70% by weight of an internal treatment agent, 0.1 to 70% by weight of a surface treatment agent, and 0.1 to 20% by weight of a filling treatment agent so as to satisfy a total content of 100% by weight. include These contents are for optimizing the above-mentioned effects, and it is difficult to secure the above-mentioned effects when the contents are out of this range.

Plant conservation treatment method

According to one embodiment of the present invention, preservation treatment of plants is performed using the composition for preservation treatment of plants.

First, as a pretreatment for plant preservation treatment, the surface is cleaned with distilled water and then dried in a cool place below 20 ° C. For drying, either hot air or cold air drying can be used, and in this embodiment, drying was performed with an air gun.

Pre-treated plants can be used in both a method of mixing all of the above-described preservation compositions and a method of using each step.

As an example, after putting the pretreated plant into a container, injecting the plant preservation treatment composition, and after a certain period of time, take it out and dry it in a cool place below 20 ° C. to complete the treatment process for preservation. At this time, the solvent used for the antibacterial agent, internal treatment agent, surface treatment agent, and filling treatment agent includes water, ethanol, and isopropanol, and all of them have excellent mixing properties, so that the above compositions can be mixed and used at once.

At this time, the immersion is performed at room temperature within 1 minute to 1 hour, so that the plant can be preserved within a short time.

As another example, the present invention may be performed by dividing the treatment process into stages.

Specifically, the pretreated plants complete the treatment process for preservation through drying after treatment with an antibacterial agent, drying after treatment with an internal treatment agent, drying after treatment with a surface treatment agent, and drying after treatment with a filling treatment agent. do.

The treatment in each step is performed by putting the plant in the container and holding it for a certain time. At this time, the time is carried out at room temperature within 1 minute to 1 hour, so that the plants in the container can be sufficiently contained. If the time is exceeded, the color or shape of the plant may change.

Drying in each step is dried in a cool place below 20 ° C.

Preservation treatment according to the present invention can be performed within one day, and time can be reduced compared to the conventional treatment for several days.

herbarium

According to one embodiment of the present invention, the preserved plant may be prepared as a specimen using a resin or a preservation solution.

The plant specimen herein refers to a form preserved by curing resin or preservative solution without changing the shape and color of flowers, leaves, stems or roots of plants.

As an example, the production of a plant sample using resin is prepared by putting a plant in a transparent container, injecting a resin in a liquid form, and then post-curing. Specimens prepared through this step are called plant resin specimens.

Any resin that can be used is curable and maintains transparency after curing. As the curing method, all of thermal curing, room temperature curing, and light curing are possible. However, a resin that does not cause denaturation or transformation of plants due to excessive heat generation or shrinkage after light curing is preferred.

For example, the resin may be at least one selected from the group consisting of epoxy resins, urethane resins, acrylate resins, and silicone resins.

As another example, the preparation of a plant sample using a preservative solution is prepared by putting a plant in a transparent container, injecting a liquid preservative solution, and then sealing it. Specimens prepared through this step are referred to as plant herbarium specimens.

The preservative solution may be at least one selected from the group consisting of mineral oil, paraffin oil, and silicone oil that maintains transparency.

The plant specimen of the present invention not only prevents discoloration by blocking oxygen from reaching from the outside, but also can be semi-permanently or permanently preserved in an intact state as if alive.

[Example]

Hereinafter, the present invention will be described in detail through examples of the present invention, but they do not limit the scope of the present invention.

[Examples and Comparative Examples]

10 parts by weight of Plantago asiatica leaves were prepared, washed with distilled water for 5 minutes, and then dried with an air gun at 20° C. for 10 minutes.

Subsequently, it was immersed in an antibacterial agent for 1 minute, immersed in an internal treatment agent for 5 minutes, immersed in a surface treatment agent for 5 minutes, and immersed in a filler for 1 minute. After immersion in each step, plantain leaves were dried with an air gun and dried at 20 ° C. for 10 minutes.

At this time, Example 7 includes the composition of Example 1, but after mixing the antibacterial agent, internal treatment agent, surface treatment agent, and filling treatment agent at once, plantain leaves are immersed for 5 minutes, dried with an air gun, and dried at 20 ° C. for 10 minutes. A process was performed.

steps (parts by weight) Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 wash Distilled water 100 100 100 100 100 100 antibacterial NaOCl 1% aqueous solution 10 10 10 10 10 10 internal treatment agent acetone 10 - 10 10 10 10 ethanol 22 22 22 22 22 22 acetic acid - 10 - - - - surface treatment agent PVP 20 20 - - - - PVA - - 20 - - - PAA - - - 20 20 - cellulose - - - - - 20 ethanol 15 15 15 10 10 10 IPA 15 15 15 10 10 10 water - - - 10 10 10 filling agent nano silica 2 2 2 2 2 2 ethanol 2 2 2 2 2 2 IPA 2 2 2 2 2 2 water 2 2 2 2 2 2

steps (parts by weight) Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 wash Distilled water 100 100 100 100 100 antibacterial NaOCl - 10 10 10 10 internal treatment agent acetone 10 - 10 10 10 ethanol 22 - 22 22 22 surface treatment agent PVP 20 20 - 20 - PEG - - - - 20 ethanol 15 15 - 15 15 IPA 15 15 - 15 - water - 10 - - 15 filling agent nano silica 2 2 2 - 2 ethanol 2 2 2 - 2 IPA 2 2 2 - 2 water 2 2 2 - 2

[Test Example 1] Visual evaluation

The shape and color change of plantain leaves treated in Examples and Comparative Examples was confirmed, and the results are shown in Table 3 below.

form color Shrink crumbling discoloration Corruption Example 1 1.2 1.1 doesn't exist doesn't exist Example 2 1.2 1.1 doesn't exist doesn't exist Example 3 1.3 1.2 doesn't exist doesn't exist Example 4 1.3 1.6 doesn't exist doesn't exist Example 5 1.5 1.3 doesn't exist doesn't exist Example 6 1.6 1.4 doesn't exist doesn't exist Example 7 1.3 1.1 doesn't exist doesn't exist Comparative Example 1 1.3 1.2 generation generation Comparative Example 2 2.1 2.3 generation doesn't exist Comparative Example 3 3.5 3.6 generation doesn't exist Comparative Example 4 2.9 2.8 generation doesn't exist Comparative Example 5 2.3 2.2 generation doesn't exist <Shrinkage/crash graded into 5 levels>
severe... … … … Almost none
5... 4… 3... 2… One <After 10 days at room temperature
Observe with the naked eye>

Looking at Table 3, it can be seen that the shape and color of plantain leaves are maintained when treated with the method according to the present invention. In addition, comparing Examples 1 and 7, the same effect could be ensured even if each processing agent was mixed at once.

As in Comparative Example 1, when the antimicrobial agent was not treated, corruption occurred, and as in Comparative Examples 2 and 4, when each treatment step was partially excluded, deformation occurred in shape and color. In addition, in the case of Comparative Example 5 using PEG, there was no discoloration, but a change in shape was observed.

[Test Example 2] Evaluation of discoloration resistance

,

,

값을 색차계를 사용하여 측정하고, 색차 변화도(

)를 수학식 1로 계산하여 표 4에 나타내었다. 이때 색차 변화도(

)가 작을수록 변색이 발생하지 않음을 의미한다.Plantain leaves treated in Examples and Comparative Examples before and after treatment

,

,

The values were measured using a color difference meter, and the color difference gradient (

) was calculated by Equation 1 and shown in Table 4. At this time, the color difference gradient (

) means that discoloration does not occur as the value is small.

[Equation 1]

Example 1 0.8 Example 2 0.9 Example 3 1.1 Example 4 1.3 Example 5 1.2 Example 6 1.3 Example 7 0.9 Comparative Example 1 4.2 Comparative Example 2 3.6 Comparative Example 3 4.0 Comparative Example 4 3.2 Comparative Example 5 2.7

Looking at Table 4, it can be seen that it is more advantageous to maintain color when the processing method according to the present invention is performed.

[Test Example 3] Preparation and evaluation of plant specimens

Plant specimens were prepared using plantain leaves treated in Example 2, Comparative Example 3, and Comparative Example 5. After each plantain leaf was put into a 50ML vial, acrylic resin was injected and cured through light irradiation to prepare a plant specimen.

1 (a) to (c) are photographs of plant specimens of plantain leaves treated in Example 1, Comparative Example 3, and Comparative Example 5. Referring to FIG. 1, it can be seen that shrinkage and brittleness occurred in Comparative Example 3 in which no surface treatment agent was used, and discoloration occurred in the case of using PEG as in Comparative Example 5.

Claims (9)

delete To satisfy the total content of 100% by weight,
0.01 to 20% by weight of an antimicrobial agent containing NaOCl,
0.1 to 70% by weight of two or more internal treatment agents selected from the group consisting of acetone, ethanol and acetic acid;
0.1 to 70% by weight of a surface treatment agent containing at least one polar polymer selected from the group consisting of polyvinylpyrrolidone, polyvinyl butyral and polyacrylic acid and two mixed solvents selected from the group consisting of water, ethanol and isopropyl alcohol %, and
0.1 to 20% by weight of a filling treatment agent containing nano silica having an average particle diameter of 0.1 to 1000 nm as a filler and a mixed solvent consisting of water, ethanol and isopropyl alcohol,
A composition for preserving plants for the preparation of plant specimens.
According to claim 2,
The antimicrobial agent is a NaOCl aqueous solution, a composition for preserving plants.
delete delete A method for preserving plants comprising the step of immersing plants in the composition for preserving plants according to claim 2 and then drying them.
Treating plants with the composition for preserving plants according to claim 2 in multiple stages,
drying the plant after treatment with an antimicrobial agent;
Drying after treatment with an internal treatment agent;
Drying after treatment with a surface treatment agent, and
A plant conservation treatment method comprising the step of drying after treatment with a filling treatment agent.
A plant specimen manufacturing method comprising immersing a plant preserved according to any one of claims 6 or 7 in a resin and then curing the plant.
delete

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