CN108244093A - A kind of insect specimen color preserving agent, preparation method and color-retention method - Google Patents
A kind of insect specimen color preserving agent, preparation method and color-retention method Download PDFInfo
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- CN108244093A CN108244093A CN201810337398.6A CN201810337398A CN108244093A CN 108244093 A CN108244093 A CN 108244093A CN 201810337398 A CN201810337398 A CN 201810337398A CN 108244093 A CN108244093 A CN 108244093A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
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Abstract
The invention belongs to insect specimen color preserving agent technical fields, and in particular to a kind of insect specimen color preserving agent, preparation method and color-retention method for acting on green insect chemistry color.The insect specimen color preserving agent of the present invention includes A liquid and B liquid, A liquid combines potassium sorbate, DL tartaric acid, formaldehyde, sodium benzoate, hindered amine light stabilizer 770, absolute ethyl alcohol, for color preserving agent A liquid compatibilities, raw material is cheap and easy to get, preparation process is easy to operate, the each component division of labor is clear and definite, is collocated with each other.Potassium sorbate, sodium benzoate are widely used in the preservative of food industry；Formaldehyde is then to have strong antisepsis and sterilization performance by strong reducing property；DL tartaric acid is then as anti-oxidant synergist；Hindered amine light stabilizer 770 plays anti-light solution, enhancing antioxidant heat resistance effectiveness.UV 531 in B liquid can also play the effect of anti-light solution, main to absorb 240 340nm ultraviolet lights.
Description
Technical field
The invention belongs to insect specimen color preserving agent technical fields, and in particular to a kind of elder brother for acting on green insect chemistry color
Worm sample color preserving agent, preparation method and color-retention method.
Background technology
Insect specimen is to carry out classification of insect, study the important tool for understanding a series of subjects of insect, the information of record
More accurate, closer former live traits, are more conducive to the development of correlative study.However insect make become sample after,
Insect bodies cell is died off, and many reasons cause skin corium endochrome to be decomposed, and chemical color colour fading causes insect occur
The phenomenon that body colour is distorted.To solve this problem, from two side of oxidases and protection pigment molecular structure for inhibiting decomposition pigment
Face is started with, and a kind of novel insect body colour protective agent is developed in proposition.Function is intended to retain insect complete body colour before death, improves elder brother
The writing function of worm sample meets teaching research and research practice needs.
The most commonly used is formalin fixation liquid in existing insect color-retention method, make for different body colour insect larvaes
With different additives such as glacial acetic acid, glycerine, guarantor's color is carried out for assorted insect larvae.As by 1. white sugar 5g, glacial acetic acid 5mL, good fortune
You are Malin 4mL, and the fixation liquid that distilled water 100mL is prepared is aiming at the larva of yellow, green；2. glycerine 20mL, glacial acetic acid
4mL, formalin 4mL, distilled water 100mL are then for the dark larva such as red, brown.In addition with phenol, carbolic acid
The record of dipping specimen is configured.The advantages of above method is：Insect larvae period body colour information can be recorded；Shortcoming is：Applicable surface
It is narrow, complete life history can not be generalized to.
Existing second common of color-fixing method is exactly the waxdip fixation technology applied to dry preserved specimen, saturating using resinae
Bright or translucent binder produces artificial amber insect specimen, mainly adequately protects to schemochrome, secondly by isolation
Pigment is protected in exogenous dioxygen oxidation, disease pest invasion.With materials chemistry development embedded material also gradually from rosin to
Epoxy resin (polyester resin), polymethyl methacrylate (organic glass) transformation.The method can make insect specimen body surface directly quilt
Resin all standing, shortcoming are can not to observe fine structure, and the build of insect is also required.It takes in the production process long
It is practical also to lose pigment in most of insect bodies.Requirement of the insect specimen spread the wings to resin temperature is than tranquillization state insect specimen
Higher, the resin temperature that pours is excessively high to make wing be denaturalized and deform, the ornamental value and species taxonomy of very big influence insect.
Invention content
The object of the present invention is to provide a kind of insect specimen color preserving agent, preparation method and color-retention methods, are proposing insect mark
During one new way of this color-retention method, the shortcomings that also avoiding existing color-retention method as possible.
Technical scheme of the present invention：
A kind of insect specimen color preserving agent, including A liquid and B liquid；
The A liquid is made of the component of following molar concentration：0.015mol/L potassium sorbates, 0.01mol/L DL- wine
Stone acid, 0.005mol/L hindered amine light stabilizers -770,0.03mol/L sodium benzoates, 0.01mol/L phenylthioureas, it is used
Solvent is volume ratio 1:1:3 formalin, water and absolute ethyl alcohol, with 0.01mol/L sodium hydroxide solution tune pH to 8.0~
9.0；Wherein, formalin is the formaldehyde saturated aqueous solution of 35~40wt%；
The B liquid is the benzole soln of 0.02mol/L light stabilizers UV-531.
A kind of preparation method of insect specimen color preserving agent, step are as follows：
Volume ratio is 1 by S1:1 formalin and water mixing, then potassium sorbate and DL- tartaric acid are added, control sorb
The molar concentration of sour potassium, DL- tartaric acid in color preserving agent A liquid is respectively 0.015mol/L, 0.01mol/L, is shaken up；
S2, it is 3 to take with formalin volume ratio:1 absolute ethyl alcohol, then add hindered amine light stabilizer -770, benzoic acid
Sodium and phenylthiourea, shake up；Control mole of hindered amine light stabilizer -770, sodium benzoate, phenylthiourea in color preserving agent A liquid
Concentration is respectively 0.005mol/L, 0.03mol/L, 0.01mol/L；
Step S1 and step S2 are mixed, shaken up again by S3, with 0.01mol/L sodium hydroxide solution tune pH to 8.0~
9.0, surplus volume ratio is 1:1:The mixed liquor constant volume of 3 formalin, water and ethyl alcohol obtains insect specimen color preserving agent A liquid；
S4 adds in UV-531 in benzene, shakes up, and molar concentrations of the UV-531 in color preserving agent B liquid is 0.02mol/L, then
With benzene constant volume, color preserving agent B liquid is obtained.
A kind of color-retention method of insect specimen color preserving agent, step are as follows：
Step 1, by insect starvation two round the clock, clear up body surface, then carry out it is T-shaped cut off thoracic cavity, clear up internal organ, drop
Low bacterium damages sample；
Step 2, the insect for not yet losing consciousness is immersed into 2~5min in the A liquid equipped with insect specimen color preserving agent rapidly,
After drying, then brush the B liquid of one layer of insect specimen color preserving agent；
Step 3, the insect specimen that will be handled well, whole appearance, fixation obtain sample finished product.
T-shaped scissors mouth length and width described in step 1 should be less than insect chest segment length and wide 2/3, more than insect chest segment length
With wide 1/3.
In preparation method, color-retention method in above-mentioned insect specimen color preserving agent, the ingredient of color preserving agent is configured and using operation
It need to be carried out in the case where wearing goggles, organic steam breathing mask, emgloves.
Compared with prior art, insect specimen color preserving agent provided by the invention has following features：
(1) insect specimen color preserving agent of the invention includes A liquid and B liquid, and A liquid is by potassium sorbate, DL- tartaric acid, formaldehyde, benzene
Sodium formate, hindered amine light stabilizer -770, absolute ethyl alcohol combine, and for color preserving agent A liquid compatibilities, raw material is cheap and easy to get, prepares
Journey is easy to operate, and each component division of labor is clear and definite, is collocated with each other.
(2) potassium sorbate, sodium benzoate are widely used in the preservative of food industry；Formaldehyde is had by strong reducing property
There is strong antisepsis and sterilization performance；DL- tartaric acid is then as anti-oxidant synergist；The anti-light solution of the performance of hindered amine light stabilizer -770,
Enhance antioxidant heat resistance effectiveness；
(3) UV-531 in B liquid can also play the effect (main to absorb 240-340nm ultraviolet lights) of anti-light solution.
(4) by starvation and except internal organ can weaken the factor for leading to degenerate brown stain to greatest extent.
Specific embodiment
The present invention is described in detail With reference to embodiment, it is to be understood that protection scope of the present invention is simultaneously
It is not restricted by specific implementation.Test method without specific conditions in the following example, usually according to normal condition
Operation, the experiment material source being not specified is commercially available, due to not being related to inventive point, therefore its details step is not described in detail.
When embodiment provides numberical range, it should be appreciated that except non-present invention is otherwise noted, two ends of each numberical range
Any one numerical value can be selected between point and two endpoints.Unless otherwise defined, in the present invention all technologies for using and
Scientific terminology is identical with the normally understood meaning of those skilled in the art.Except the specific method, equipment, the material that are used in embodiment
Outside, according to the art to the grasp of the prior art and the present invention record, can also use in the embodiment of the present invention
Any method, equipment and the material of the similar or equivalent prior art of the method, equipment, material realizes the present invention.
Embodiment 1
A kind of insect specimen color preserving agent, including A liquid and B liquid, the A liquid is made of the component of following molar concentration：Sorb
Sour potassium (0.015mol/L), DL- tartaric acid (0.01mol/L), hindered amine light stabilizer -770 (0.005mol/L), sodium benzoate
(0.03mol/L), phenylthiourea (0.01mol/L), 1:1:3 formalin (35~40% formaldehyde saturated aqueous solutions), water
And absolute ethyl alcohol, with sodium hydroxide (0.01mol/L) solution tune pH to 8.0~9.0, solvent residual quantity formalin, water and second
Alcohol (1:1:3) mixed liquor constant volume.It is prepared according to following steps：
S1 indicates that quality weighs each component by following：First with 25mL pipettes pipette 19.00mL formalin and
19.00mL water stoppers bottleneck after being transferred to 50mL conical flasks A, then weighs potassium sorbate (0.2253g), DL- tartaric acid
(0.1501g) is added in above-mentioned conical flask fully dissolving and shakes up.
S2 is first pipetted after 57.00mL absolute ethyl alcohols are transferred to 100mL conical flasks B with 100mL pipettes and is stoppered bottleneck, then
Hindered amine light stabilizer -770 (0.2404g), sodium benzoate (0.4324g), phenylthiourea (0.1522g) are weighed, is added to
It states fully to dissolve in conical flask and shake up.
Formalin solution in conical flask A is poured into conical flask B, and fully shake up again, uses sodium hydroxide by S3
(0.01mol/L) solution tune pH to 8.5~9.0, surplus formalin, water and ethyl alcohol (1:1:3) mixed liquor is settled to taper
The 100mL graduation marks of bottle B obtain a green insect specimen color preserving agent A liquid for being characterized as that suspension specification is 100mL.
S4 measures about 50mL benzene with beaker, weighs UV-531 (0.6528g), the UV-531 weighed is added in beaker
Fully dissolving shakes up, then solution is transferred to 100mL volumetric flasks, is settled to 100mL with benzene, obtains color preserving agent B liquid.
Embodiment 2
A kind of insect specimen color preserving agent, including A liquid and B liquid, the A liquid is made of the component of following molar concentration：Sorb
Sour potassium (0.015mol/L), DL- tartaric acid (0.01mol/L), hindered amine light stabilizer -770 (0.005mol/L), sodium benzoate
(0.03mol/L), phenylthiourea (0.01mol/L), 1:1:3 formalin (35~40% formaldehyde saturated aqueous solutions), water
And absolute ethyl alcohol, with sodium hydroxide (0.01mol/L) solution tune pH to 8.0~9.0, solvent residual quantity formalin, water and second
Alcohol (1:1:3) mixed liquor constant volume.Preparation method is same
Embodiment 1.
Color preserving agent B liquid is not prepared, using brushing benzene as blank control.
Embodiment 3
A kind of insect specimen color preserving agent, including A liquid and B liquid, the A liquid is made of the component of following molar concentration：Sorb
Sour potassium (0.015mol/L), DL- tartaric acid (0.01mol/L), hindered amine light stabilizer -770 (0.005mol/L), sodium benzoate
(0.03mol/L), phenylthiourea (0.01mol/L), 1:1:3 formalin (35~40% formaldehyde saturated aqueous solutions), water
And absolute ethyl alcohol, with sodium hydroxide (0.01mol/L) solution tune pH to 8.0~9.0, solvent residual quantity ethyl alcohol constant volume.Preparation side
Method is the same as embodiment 1.
Using atoleine as color preserving agent B liquid.
Step 1, by the fresh insect starvation two of live body round the clock, the dirt of net body surface is cleaned, then T-shaped with operating scissors
Thoracic cavity is cut off, with the internal organ for cleaning out thoracic cavity and abdominal cavity top of tip tweezers as early as possible as possible, mainly digestive organ such as
Crop, glandular stomach, diverticulum, middle intestines etc., reduce the inside live bacterium can to sample hurtful possibility.
Step 2, the insect for not yet losing consciousness is immersed into the test tube equipped with green insect specimen color preserving agent A liquid rapidly
2~4min is picked up and is brushed one layer of color preserving agent B liquid after drying again.
Step 3, the insect specimen handled well through color preserving agent, whole appearance, fixation obtain sample finished product.
The explanation required supplementation with, in the preparation method, color-retention method in above-mentioned insect specimen color preserving agent, the ingredient of color preserving agent
It is configured and need to be carried out using operation in the case where wearing goggles, organic steam breathing mask, emgloves.Described in step 1
T-shaped scissors mouth length and width should be less than insect chest segment length and wide 2/3, more than insect chest segment length and wide 1/3.
It should be noted that, experiment different according to the formula of the insect specimen color preserving agent of green insect chemistry color used
Group is divided into 1 group of embodiment, 2 groups of embodiment, 3 groups of embodiment.
Common insect specimen as a control group one is considered as with the insect without processing after being killed with ethyl acetate poison bottle
The insect not dealt with after being dried with the insect handled according to step 1 is considered as except internal organ insect specimen as a control group
Two
By distinct methods processing insect specimen be placed on equipped with camphor, silica-gel desiccant transparent specimen box in be tamping,
Transparent specimen box is put into glass cabinet again, direct sunlight is avoided normally to preserve under room ventilation environment.The first month observes and records daily,
The whether normal of color progress is protected in observation, is then changed to carry out shooting weekly that card is stayed to record, is observed and compare the green of insect specimen
Chemical color fading time, sets observation period as a year and a half.As a result as shown in table 1, table 2, the results showed that, utilize the present invention's
The insect specimen fading time that color preserving agent is prepared is extended, and is removed internal organs and is had no substantive shadow for sample presentation posture
It rings, and removing internal organ can slow down really its brown stain speed.
In addition, 3 groups of 1 group of comparing embodiment, 2 groups of embodiment and embodiment, by color preserving agent A liquid and B liquid simultaneously using certain
There is a degree of extension fading time, strengthen protecting color effect.And compared with use atoleine carry out waxdip fixation, color preserving agent B
Liquid is to sample damage degree smaller.The waxdip fixation of atoleine can also cause some variations of insect surfaces color, with primary colors phase
Than generating larger discrepancy.
1 Asiatic migrotory locust of table (Locusta migratoria manilensis (Meyen, 1835)) (green) sample observation note
Record
The one colour fading brown stain time of control group is 3 days, and the two colour fading brown stain time of control group is 8 days
2 grasshopper of table (Acrida cinerea) sample observes and records
The one colour fading brown stain time of control group is 2 days, and the two colour fading brown stain time of control group is 4 days
Indoor 25 ± 2 DEG C of humidity 74~78% of storage temperature
Although preferred embodiments of the present invention have been described, but those skilled in the art once know basic creation
Concept, then additional changes and modifications may be made to these embodiments.So appended claims are intended to be construed to include preferably
Embodiment and all change and modification fallen within the scope of the present invention.
Obviously, various changes and modifications can be made to the invention without departing from essence of the invention by those skilled in the art
God and range.In this way, if these modifications and changes of the present invention belongs to the range of the claims in the present invention and its equivalent technologies
Within, then the present invention is also intended to include these modifications and variations.
Claims (4)
1. a kind of insect specimen color preserving agent, which is characterized in that the color preserving agent includes A liquid and B liquid；
The A liquid is made of the component of following molar concentration：0.015mol/L potassium sorbates, 0.01mol/L DL- tartaric acid,
0.005mol/L hindered amine light stabilizers -770,0.03mol/L sodium benzoates, 0.01mol/L phenylthioureas, solvent used are
Volume ratio 1:1:3 formalin, water and absolute ethyl alcohol, with 0.01mol/L sodium hydroxide solution tune pH to 8.0~9.0；Its
In, formalin is the formaldehyde saturated aqueous solution of 35~40wt%；
The B liquid is the benzole soln of 0.02mol/L light stabilizers UV-531.
2. a kind of preparation method of insect specimen color preserving agent, which is characterized in that step is as follows：
Volume ratio is 1 by S1:1 formalin and water mixing, then potassium sorbate and DL- tartaric acid are added, control sorbic acid
The molar concentration of potassium, DL- tartaric acid in color preserving agent A liquid is respectively 0.015mol/L, 0.01mol/L, is shaken up；
S2, it is 3 to take with formalin volume ratio:1 absolute ethyl alcohol, then add hindered amine light stabilizer -770, sodium benzoate and
Phenylthiourea shakes up；Control the molar concentration of hindered amine light stabilizer -770, sodium benzoate, phenylthiourea in color preserving agent A liquid
Respectively 0.005mol/L, 0.03mol/L, 0.01mol/L；
Step S1 and step S2 is mixed, shaken up again by S3, remaining with 0.01mol/L sodium hydroxide solution tune pH to 8.0~9.0
Amount volume ratio is 1:1:The mixed liquor constant volume of 3 formalin, water and ethyl alcohol obtains insect specimen color preserving agent A liquid；
S4 adds in UV-531 in benzene, shakes up, and molar concentrations of the UV-531 in color preserving agent B liquid is 0.02mol/L, then uses benzene
Constant volume obtains color preserving agent B liquid.
3. a kind of color-retention method of insect specimen color preserving agent, which is characterized in that step is as follows：
Step 1, by insect starvation two round the clock, clear up body surface, then carry out it is T-shaped cut off thoracic cavity, clear up internal organ, reduce thin
Bacterium damages sample；
Step 2, the insect for not yet losing consciousness is immersed into 2~5min in the A liquid equipped with insect specimen color preserving agent rapidly, dried
Afterwards, then the B liquid of one layer of insect specimen color preserving agent is brushed；
Step 3, the insect specimen that will be handled well, whole appearance, fixation obtain sample finished product.
4. the color-retention method of a kind of insect specimen color preserving agent according to claim 3, which is characterized in that described in step 1
T-shaped scissors mouth length and width should be less than insect chest segment length and wide 2/3, more than insect chest segment length and wide 1/3.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113115763A (en) * | 2019-12-30 | 2021-07-16 | 上海市林业总站 | Lepidoptera insect dried specimen manufacturing method |
| CN113744617A (en) * | 2021-09-17 | 2021-12-03 | 海南省环境科学研究院 | Method for preparing monolith of acidic sulfate soil |
| CN116495994A (en) * | 2023-04-14 | 2023-07-28 | 江苏盛鸿电工器材有限公司 | Production process method of ultrathin electronic grade glass fiber cloth |
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Application publication date: 20180706 |