CN109221096A - A kind of wild russule humid preparation preservation liquid - Google Patents
A kind of wild russule humid preparation preservation liquid Download PDFInfo
- Publication number
- CN109221096A CN109221096A CN201811172155.8A CN201811172155A CN109221096A CN 109221096 A CN109221096 A CN 109221096A CN 201811172155 A CN201811172155 A CN 201811172155A CN 109221096 A CN109221096 A CN 109221096A
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- russule
- color
- calcium chloride
- formaldehyde
- acetic acid
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- 238000002360 preparation method Methods 0.000 title claims abstract description 25
- 239000007788 liquid Substances 0.000 title claims abstract description 22
- 238000004321 preservation Methods 0.000 title abstract description 15
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims abstract description 52
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 47
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims abstract description 25
- 229910001628 calcium chloride Inorganic materials 0.000 claims abstract description 24
- 239000001110 calcium chloride Substances 0.000 claims abstract description 24
- 229960000583 acetic acid Drugs 0.000 claims abstract description 23
- 239000012362 glacial acetic acid Substances 0.000 claims abstract description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000012153 distilled water Substances 0.000 claims abstract description 10
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 claims abstract description 7
- 230000000694 effects Effects 0.000 abstract description 12
- 230000007774 longterm Effects 0.000 abstract description 3
- 239000000523 sample Substances 0.000 description 9
- 241000196324 Embryophyta Species 0.000 description 8
- 239000000049 pigment Substances 0.000 description 8
- 230000008859 change Effects 0.000 description 7
- 238000000034 method Methods 0.000 description 6
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 5
- 239000000834 fixative Substances 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- 239000003112 inhibitor Substances 0.000 description 3
- 238000006552 photochemical reaction Methods 0.000 description 3
- 239000000654 additive Substances 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 238000005842 biochemical reaction Methods 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 210000000349 chromosome Anatomy 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 235000019441 ethanol Nutrition 0.000 description 2
- 230000005284 excitation Effects 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- 241000222485 Agaricales Species 0.000 description 1
- 241000707825 Argyrosomus regius Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 208000003556 Dry Eye Syndromes Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000221987 Russula Species 0.000 description 1
- 241000221986 Russulaceae Species 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000003044 adaptive effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000002845 discoloration Methods 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000005562 fading Methods 0.000 description 1
- 125000002485 formyl group Chemical class [H]C(*)=O 0.000 description 1
- 238000009499 grossing Methods 0.000 description 1
- 230000005283 ground state Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- KBPHJBAIARWVSC-RGZFRNHPSA-N lutein Chemical compound C([C@H](O)CC=1C)C(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\[C@H]1C(C)=C[C@H](O)CC1(C)C KBPHJBAIARWVSC-RGZFRNHPSA-N 0.000 description 1
- 229960005375 lutein Drugs 0.000 description 1
- ORAKUVXRZWMARG-WZLJTJAWSA-N lutein Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=CC(O)CC2(C)C)C ORAKUVXRZWMARG-WZLJTJAWSA-N 0.000 description 1
- 235000012680 lutein Nutrition 0.000 description 1
- 239000001656 lutein Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- KBPHJBAIARWVSC-XQIHNALSSA-N trans-lutein Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CC(O)CC1(C)C)C=CC=C(/C)C=CC2C(=CC(O)CC2(C)C)C KBPHJBAIARWVSC-XQIHNALSSA-N 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 239000005418 vegetable material Substances 0.000 description 1
- 239000000052 vinegar Substances 0.000 description 1
- 235000021419 vinegar Nutrition 0.000 description 1
- FJHBOVDFOQMZRV-XQIHNALSSA-N xanthophyll Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CC(O)CC1(C)C)C=CC=C(/C)C=CC2C=C(C)C(O)CC2(C)C FJHBOVDFOQMZRV-XQIHNALSSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
-
- G—PHYSICS
- G09—EDUCATION; CRYPTOGRAPHY; DISPLAY; ADVERTISING; SEALS
- G09B—EDUCATIONAL OR DEMONSTRATION APPLIANCES; APPLIANCES FOR TEACHING, OR COMMUNICATING WITH, THE BLIND, DEAF OR MUTE; MODELS; PLANETARIA; GLOBES; MAPS; DIAGRAMS
- G09B23/00—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes
- G09B23/38—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes for botany
Abstract
The invention discloses a kind of wild russule humid preparations to save liquid, including calcium chloride, formaldehyde, glacial acetic acid and distilled water；Every 100ml is saved in liquid, and the calcium chloride content is 8~12g, and the content of formaldehyde is 4~6ml, and the glacial acetic acid content is 4~6ml, remaining is distilled water；The present invention can to color, form is preferable or color, form are imperfect but have the effect of the wild russule of ornamental value with long-term preservation, color that can be initial to russule, form, even russule itself lines have quite complete preservation, people understood and watch valuable wild russule be of great significance.
Description
Technical field
The present invention relates to a kind of humid preparations to save liquid, and specifically a kind of wild russule humid preparation saves liquid, belongs to life
Object preparation of specimen and Techniques of preserving field.
Background technique
Russule (Russule) popular name red mushroom belongs to Mycophyta door, Agaricales, Russulaceae.The fructification of russule it is small or
Medium, 4~7cm of diameter of cap, the shape of cap is hemispherical to flat hemispherical when initial stage, and the later period is gradually open and flat, middle part to
Lower recess, general that rose-red or vermilion is presented, slightly sticky when moistening, edge-smoothing is without a rib.Bacterial context is white, thicker.Bacterium
Pleat near-white grows to tip straight, slightly close to have bifurcated, isometric or Length discrepancy.Stem length is generally 4~7cm, thickness journey
1.6~1.5cm is spent, cylindrical, white band pink, slightly gauffer are internal soft and hollow.It is edible, but spicy, slightly
Hardship, being cooked after drying can eat.Russula is widely distributed in China, and other than Shanghai, Macao two places, there is note in remaining province
It carries, is distributed mainly on the ground such as Liaoning, Shaanxi, Jiangsu, Hubei, Sichuan, Fujian, Guangxi, Yunnan.
Humid preparation can save the form and color of plant very well, be a kind of teaching aid that comparison is lively, vivid.Simultaneously
Humid preparation production method is simple, and utensil and reagent used are easy to prepare, and in various regions universities and colleges, middle school is easy to carry out experiment.
It is still the color protection of popular problem, especially humid preparation today for humid preparation preservation, is still difficult point.Generally
There is following four situation: 1. chemical changes；2. biochemical reaction；3. biology variation；4. photochemical reaction.As follows respectively: chemistry becomes
Change: is changed by the quality that a chemical reaction causes.For example, carrotene is that one kind is distributed widely in plant leaf blade, belong to
In the stronger substance of activity, it is easy to react with the either stronger species of oxidisability of the oxygen in air.Pigment after reaction
Being destroyed makes its colour fading.Biochemical reaction: being cannot be timely and effectively by the cell in sample in fixed preparation due to operator
It kills, causes cell to expand, rupture during being soaked, sample is made to change colour.In addition, the substance in cytoplasm flows out,
The effect of color protection to russule for being soaked liquid may be changed.Biology variation: the destruction of microorganism, microorganism be a kind of adaptive faculty and
The all especially strong species of reproductive capacity will mushroom out once there is the environment of adaptation.In the manufacturing process of humid preparation inevitably
Have a careless omission, microorganism may take advantage of the occasion growth and breeding, destroy sample material, eventually change the color even form of sample.
In addition, microorganism itself also has certain color developing effect, sample discoloration is also resulted in.Photochemical reaction is familiar at us
Pigment in, can decompose in light there are many pigment.For example, lutein, but be substantially because pigment molecular is being absorbed into light
According to excitation state that is rear, converting pigment molecular from ground state, the pigment of excitation state is easier to that photochemical reaction occurs, and pigment is caused to split
Solution, to change the color of humid preparation.To sum up, humid preparation is that fresh vegetable material is immersed in chemicals
In the solution of preparation, it is made to keep a kind of plant storaging method of the color of script and morphosis.This humid preparation has
The features such as three-dimensional sense is strong, and form is true to nature, bright in colour, it is Plant Taxonomy, investigation of plant resources, plant protection, floral region
System's research etc. essential scientific basis.
Therefore, according to the qualitative change mechanism of sample, there is the prevention method below for property: the selection of 1. environment；2. adding
Add the selection of agent；3. the selection of fixative；The selection of environment: aeration-drying, low temperature no light, radiationless source；The choosing of additive
Select: general additive is divided into two kinds, inhibitor and promotor.There can be enzymatic reaction in sample, addition inhibitor can inhibit
The generation of reaction, to achieve the effect that preservation.But inhibitor has certain use time, so the method for this preservation is deposited centainly
Defect；The selection of fixative: data shows that ethyl alcohol and formalin are common fixative, and concentration used depends on the circumstances,
In general, alcohol concentration 70%, formalin are usually 5% for plant specimen, zoological specimens 10%.
In addition, dry preserved specimen is obtained by the less animal or plant production of water content.The main limitation of dry preserved specimen
Be: 1. dry-eye disease organic matter contents are more, can be used as the raw material of moth, can also become the good medium that mould is propagated；
2. the later period will have special responsible person that [13] are inspected periodically and cleared up to dry preserved specimen.So relative to humid preparation and
Speech, dry preserved specimen need a large amount of man power and material in preservation and maintenance；And dry preserved specimen is not soaked mark yet in effect
This effect is good, humid preparation for sample no matter in form or color all closer to primitive color；Operationally soak
Sample processed is even more the production for being better than dry preserved specimen, is operated simpler.To sum up, humid preparation either later maintenance,
Preservation effect and operating aspect are all more better than dry preserved specimen.
Summary of the invention
The purpose of the present invention is a kind of wild russule humid preparations to save liquid, which can be to rare, color, form
Preferably and have the effect of that the wild russule of ornamental value has preferable long-term preservation, is provided for successors and understand ornamental machine
Meeting has also done meagre contribution for national resources culture preservation.
In order to reach above-mentioned technical purpose, the technical scheme is that
A kind of wild russule humid preparation preservation liquid, it is characterised in that including calcium chloride, formaldehyde, glacial acetic acid and distilled water.
Influence of the calcium chloride dosage to russule coloration:
The dosage of every group of calcium chloride is respectively 0,5,10,15,20g, does parallel test twice.Test fixed formaldehyde and ice vinegar
Sour dosage is respectively 5.0ml, 5.0ml.As shown in Figure 1, when the dosage of calcium chloride increases to 20g from 0g, the preservation color of russule
Degree is first to rise then to decline.For first segment when the dosage of calcium chloride is between 0g~10g, the coloration of russule is to rise, the
Decline when two sections of calcium chloride dosages are between 10g~20g.Therefore from overall consideration, when calcium chloride dosage is 10g, russule color
Degree reaches maximum value, is 9.51.A kind of main agents of the calcium chloride as color protection can destroy cap surface red when concentration raising
Pigment declines its coloration.
Influence of the formaldehyde dosage to russule coloration:
The dosage of setting calcium chloride and glacial acetic acid is respectively 10.0g, 5.0ml, the dosage for studying formaldehyde is respectively 0,1,2,
3, when 4,5,6,7,8,9ml, save liquid to russule color protection influence (volume fraction 0% shared by formaldehyde, 1%, 2%, 3%, 4%,
5%, 6%, 7%, 8%, 9%), the total volume for saving liquid is 100ml.As shown in Fig. 2, with the increase of formaldehyde dosage, russule
Coloration first rise and decline afterwards.First stage, when formaldehyde volume fraction is from 0~5%, russule coloration rises；Second stage, first
When aldehyde dosage is from 5%~9%, russule coloration is gradually reduced.In setting calcium chloride dosage in 10.0g, glacial acetic acid dosage is in 5.0ml
When, formaldehyde volume fraction is 5% at this time, and russule coloration reaches maximum value 9.5.Suitable formaldehyde can make protein denaturation, lose
Original physiological function is to reach corrosion-resistant effect, and when concentration improves, meeting considerable damage edible mushroom epidermal tissue makes its change
Deformation color.
Influence of the glacial acetic acid dosage to russule coloration:
The dosage of setting calcium chloride and formaldehyde is respectively 10.0g, 5.0ml, the dosage for studying glacial acetic acid is respectively 0,1,2,
3, when 4,5,6,7,8,9ml, save liquid to russule color protection influence (volume fraction 0% shared by glacial acetic acid, 1%, 2%, 3%,
4%, 5%, 6%, 7%, 8%, 9%), the total volume for saving liquid is 100ml.As shown in figure 3, with the increasing of glacial acetic acid dosage
Add, the coloration of russule first rises to be declined afterwards.First stage, when glacial acetic acid volume fraction is from 0~5%, russule coloration rises；The
Two-stage, when glacial acetic acid dosage is from 5%~9%, russule coloration is gradually reduced.It therefore, is 5%, i.e. ice in formaldehyde volume fraction
For acetic acid dosage in 5.0ml or so, russule coloration reaches maximum value 9.44.Suitable glacial acetic acid is as fixative, when concentration mentions
Height is sprawled conducive to cell expansion, chromosome, but it is lost to easily lead to cell rupture, chromosome.
Obtain: every 100ml is saved in liquid, and the calcium chloride content is 8~12g, and the content of formaldehyde is 4~6ml, described
Glacial acetic acid content is 4~6ml, remaining is distilled water.Preferably, the calcium chloride content is 10g, the content of formaldehyde is
5ml, the glacial acetic acid content are 5ml.
The present invention can to color, form is preferable or color, form are imperfect but wild red with ornamental value
Mushroom has the effect of long-term preservation, color that can be initial to russule, form, even russule itself lines have it is quite complete
Whole preservation understands people and watches valuable wild russule and be of great significance.
Detailed description of the invention
Fig. 1 is the line chart that calcium chloride dosage influences russule coloration.
Fig. 2 is the line chart that formaldehyde dosage influences russule coloration.
Fig. 3 is the line chart that glacial acetic acid dosage influences russule coloration.
Specific embodiment
The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.
Using the wild russule of Yu Shan as experimental material, humid preparation is made, the total coloration of russule is 10.By russule distilled water handle
The soil on surface and other Fouling Cleanings are clean, are sure not to damage russule in the process of cleaning, in order to avoid destroy its initial shape
Shape.It can first be soaked with spray thrower, be then rinsed with slow flowing water；Russule is put in ventilation nature after wash clean
It dries.
Design level coding and factor corresponding relationship, as shown in table 1:
1 level code of table and factor corresponding relationship
The wild russule humid preparation of embodiment 1-13 saves liquid, including calcium chloride, formaldehyde, glacial acetic acid and distilled water, often
100ml is saved in liquid, and the calcium chloride, formaldehyde, glacial acetic acid content are shown in Table 2, remaining is distilled water.Colour measurement equipment be it is complete from
Dynamic chromascope.
When processing, (total volume for saving liquid is fixed as 100ml) is added in calcium chloride first, adds formaldehyde, glacial acetic acid.
(meeting heat release when being added in distilled water due to calcium chloride needs to return to room temperature until solution after addition) then clean will dry red
Mushroom is put into the calcium chloride solution of various concentration, is immersed in and saves in liquid, (since buoyancy is pushed down with glass slide when necessary)
Glass cover is finally covered to be sealed with paraffin.It is placed at shady and cool ventilation, observation saves the color change situation of russule in liquid
And it records.Russule primary colors is set to 10, and color dodge increases less than 10 and is then greater than 10, then carry out respective data record.
The different embodiment russule effect of color protection of table 2
As seen from the above table, the calcium chloride content is 10g, content of formaldehyde 5ml, when glacial acetic acid content is 5ml, russule color
Degree reaches maximum value, is 9.54.
Above-described embodiment is not limit the invention in any way, all to be obtained by the way of equivalent substitution or equivalent transformation
Technical solution fall within the scope of protection of the present invention.
Claims (2)
1. a kind of wild russule humid preparation saves liquid, it is characterised in that including calcium chloride, formaldehyde, glacial acetic acid and distilled water；Often
100ml save liquid in, the calcium chloride content be 8~12g, the content of formaldehyde be 4~6ml, the glacial acetic acid content be 4~
6ml, remaining is distilled water.
2. a kind of wild russule humid preparation according to claim 1 saves liquid, it is characterised in that: the calcium chloride content
For 10g, the content of formaldehyde is 5ml, and the glacial acetic acid content is 5ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811172155.8A CN109221096A (en) | 2018-10-09 | 2018-10-09 | A kind of wild russule humid preparation preservation liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811172155.8A CN109221096A (en) | 2018-10-09 | 2018-10-09 | A kind of wild russule humid preparation preservation liquid |
Publications (1)
| Publication Number | Publication Date |
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| CN109221096A true CN109221096A (en) | 2019-01-18 |
Family
ID=65054965
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811172155.8A Pending CN109221096A (en) | 2018-10-09 | 2018-10-09 | A kind of wild russule humid preparation preservation liquid |
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| Country | Link |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113545339A (en) * | 2021-07-12 | 2021-10-26 | 中国林业科学研究院亚热带林业研究所 | Preparation method of bamboo shoot soaked specimen |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0578201A (en) * | 1991-05-28 | 1993-03-30 | Res Inst For Prod Dev | Specimen of fruit body of mushroom, its production and resin solution used for the same production |
| CN105594689A (en) * | 2014-11-24 | 2016-05-25 | 雷印平 | Lentinula edodes humid preparation producing and preserving method |
| CN105685019A (en) * | 2014-11-24 | 2016-06-22 | 雷印平 | Oyster mushroom soaked specimen making and preserving method |
| CN106605650A (en) * | 2015-10-25 | 2017-05-03 | 雷印平 | Method for manufacture and preservation of lentinus edodes liquid-preserved specimen |
| CN106605652A (en) * | 2015-10-25 | 2017-05-03 | 雷印平 | Pleurotus ostreatus immersion specimen production and storage method |
-
2018
- 2018-10-09 CN CN201811172155.8A patent/CN109221096A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0578201A (en) * | 1991-05-28 | 1993-03-30 | Res Inst For Prod Dev | Specimen of fruit body of mushroom, its production and resin solution used for the same production |
| CN105594689A (en) * | 2014-11-24 | 2016-05-25 | 雷印平 | Lentinula edodes humid preparation producing and preserving method |
| CN105685019A (en) * | 2014-11-24 | 2016-06-22 | 雷印平 | Oyster mushroom soaked specimen making and preserving method |
| CN106605650A (en) * | 2015-10-25 | 2017-05-03 | 雷印平 | Method for manufacture and preservation of lentinus edodes liquid-preserved specimen |
| CN106605652A (en) * | 2015-10-25 | 2017-05-03 | 雷印平 | Pleurotus ostreatus immersion specimen production and storage method |
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| Title |
|---|
| 刘利兵 主编: "《实验基础医学》", 31 July 2009, 第四军医大学出版社 * |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113545339A (en) * | 2021-07-12 | 2021-10-26 | 中国林业科学研究院亚热带林业研究所 | Preparation method of bamboo shoot soaked specimen |
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Application publication date: 20190118 |