CN106605650A - Method for manufacture and preservation of lentinus edodes liquid-preserved specimen - Google Patents
Method for manufacture and preservation of lentinus edodes liquid-preserved specimen Download PDFInfo
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- CN106605650A CN106605650A CN201510693899.4A CN201510693899A CN106605650A CN 106605650 A CN106605650 A CN 106605650A CN 201510693899 A CN201510693899 A CN 201510693899A CN 106605650 A CN106605650 A CN 106605650A
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- lentinus edodes
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- lentinus edodess
- soaked
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Abstract
The invention discloses a method for manufacture and preservation of a lentinus edodes liquid-preserved specimen. The method comprises the following steps: (1) selecting fresh yellow lentinus edodes free of plant diseases or insect pests, (2) putting selected lentinus edodes in a stationary solution for soaking for 15 h; (3) taking lentinus edodes out, and putting the obtained lentinus edodes in a preservation liquid for soaking for 15 h; and (4) putting the lentinus edodes sporocarp soaked by the preservation liquid in a specimen bottle according to the natural state, pressing the sporocarp with a glass sheet to prevent the sporocarp from floating out of the liquid surface, pouring a prepared soaking solution, sealing the bottle with a sealing film, writing a label, attaching the label to the specimen bottle, storing the obtained specimen bottle at the room temperature, and preventing direct sunlight. According to the method, color and shape characteristics of lentinus edodes can be well maintained.
Description
Technical field
The present invention relates to Lentinus Edodess preservation field, specially a kind of Lentinus Edodess humid preparation making and store method.
Background technology
Gansu Province is located in motherland northwest innerland, because high and cold by China's SE Monsoon, Qinghai-Tibet and cover new arid three macroclimate feature and affected, the warm south of Gansu Province mountain region of moistening, gently semiarid loess plateau, changes in temperature drastic change and four, Gannan mountain region of the Hexi Corridor of arid and high and cold moistening etc. fundamental region are defined.Polytype ecological environment provides different natural endowments for all kinds of macro fungis growth promoter.In recent years, some scholars are investigated Gansu macro fungis.But it mostly is regional sex investigation or the resource investigation to a certain particular type macro fungis lacks systemic, comprehensive investigation.In order to grasp the species and its ecologicaI distribution of Gansu Province's Macro-Fungi Resource, it is necessary to carry out extensive field study in the range of Ben Sheng, substantial amounts of specimen is collected, and carry out sort research work.
The content of the invention
The present invention is directed to above weak point, there is provided a kind of Lentinus Edodess humid preparation makes and store method, can preferably keep color and luster and the morphological characteristic of Lentinus Edodess.
The technical solution adopted for the present invention to solve the technical problems is：A kind of Lentinus Edodess humid preparation makes and store method, comprising having the following steps：
（1）Select the yellow Lentinus Edodess of fresh no disease and pests harm；
（2）The Lentinus Edodess for choosing are put in fixative liquid and is soaked 15 hours；
（3）Lentinus Edodess are pulled out, is put in preservation liquid and is soaked 15 hours；
（4）To be put in Specimen bottle according to its natural form with the soaked mushroom fruiting body of liquid is preserved, and be pressed on sporophore with sheet glass, in case liquid level is exposed in floating, it is subsequently poured into the soak for preparing, and bottleneck is sealed with sealed membrane, and label is finished writing, it is affixed on Specimen bottle, put and preserve at room temperature, it is to avoid direct sunlight.
The formula of the fixative is the % ethanol 15ml of formaldehyde 2.5ml, distilled water 82.5ml, 95,
The preservation formula of liquid is formaldehyde 10ml, glacial acetic acid 5ml, 10%Cacl2Aqueous solution 10ml, distilled water 75ml.
The invention has the beneficial effects as follows：When dipping specimen is made, sporophore original color how is kept to be that key issue is located, glacial acetic acid is placed in the preservation liquid of the present invention, and glacial acetic acid has positive effect in terms of keeping sporophore original color to improve soak definition, therefore can preferably promote the preservation of Lentinus Edodess specimen.
Specific embodiment
Embodiment
A kind of Lentinus Edodess humid preparation makes and store method, comprising having the following steps：
（1）Select the yellow Lentinus Edodess of fresh no disease and pests harm；
（2）The Lentinus Edodess for choosing are put in fixative liquid and is soaked 24 hours, the formula of the fixative is the % ethanol 15ml of formaldehyde 2.5ml, distilled water 82.5ml, 95；
（3）Lentinus Edodess are pulled out, is put in preservation liquid and is soaked 15 hours, formaldehyde 10ml, glacial acetic acid 5ml, 10%Cacl2Aqueous solution 10ml, distilled water 75ml；
（4）To be put in Specimen bottle according to its natural form with the soaked mushroom fruiting body of liquid is preserved, and be pressed on sporophore with sheet glass, in case liquid level is exposed in floating, it is subsequently poured into the soak for preparing, and bottleneck is sealed with sealed membrane, and label is finished writing, it is affixed on Specimen bottle, put and preserve at room temperature, it is to avoid direct sunlight.
Claims (3)
1. a kind of Lentinus Edodess humid preparation makes and store method, it is characterised in that：Comprising having the following steps：
（1）Select the yellow Lentinus Edodess of fresh no disease and pests harm；
（2）The Lentinus Edodess for choosing are put in fixative and is soaked 24 hours；
（3）Lentinus Edodess are pulled out, is put in preservation liquid and is soaked 15 hours；
（4）To be put in Specimen bottle according to its natural form with the soaked mushroom fruiting body of liquid is preserved, and be pressed on sporophore with sheet glass, in case liquid level is exposed in floating, it is subsequently poured into the soak for preparing, and bottleneck is sealed with sealed membrane, and label is finished writing, it is affixed on Specimen bottle, put and preserve at room temperature, it is to avoid direct sunlight.
2. the making of Lentinus Edodess humid preparation according to claim 1 and store method, it is characterised in that：The formula of the fixative is：The % ethanol 15ml of formaldehyde 2.5ml, distilled water 82.5ml, 95.
3. the making of Lentinus Edodess humid preparation according to claim 1 and store method, it is characterised in that the preservation formula of liquid is formaldehyde 10ml, glacial acetic acid 5ml, 10%Cacl2Aqueous solution 10ml, distilled water 75ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510693899.4A CN106605650A (en) | 2015-10-25 | 2015-10-25 | Method for manufacture and preservation of lentinus edodes liquid-preserved specimen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510693899.4A CN106605650A (en) | 2015-10-25 | 2015-10-25 | Method for manufacture and preservation of lentinus edodes liquid-preserved specimen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106605650A true CN106605650A (en) | 2017-05-03 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510693899.4A Pending CN106605650A (en) | 2015-10-25 | 2015-10-25 | Method for manufacture and preservation of lentinus edodes liquid-preserved specimen |
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| CN (1) | CN106605650A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109221096A (en) * | 2018-10-09 | 2019-01-18 | 常熟理工学院 | A kind of wild russule humid preparation preservation liquid |
-
2015
- 2015-10-25 CN CN201510693899.4A patent/CN106605650A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109221096A (en) * | 2018-10-09 | 2019-01-18 | 常熟理工学院 | A kind of wild russule humid preparation preservation liquid |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170503 |
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| WD01 | Invention patent application deemed withdrawn after publication |