CN113115763A

CN113115763A - Lepidoptera insect dried specimen manufacturing method

Info

Publication number: CN113115763A
Application number: CN201911403346.5A
Authority: CN
Inventors: 李玉秀; 陈锦峰; 杨储丰; 王焱
Original assignee: Shanghai Forestry Central Station
Current assignee: Shanghai Forestry Central Station
Priority date: 2019-12-30
Filing date: 2019-12-30
Publication date: 2021-07-16

Abstract

The dried lepidopterous insect specimen is prepared and stored in a dry environment by adopting a drying agent, a mildew preventive and the like, has strict requirements on the environment, needs to be matched with temperature and humidity control equipment, is easy to damp and damage due to moisture absorption and the like once being exposed in the air for a long time, and has low protection performance and high specimen protection cost. The invention provides a novel preparation method of a dried specimen, which is characterized in that a permeation film-forming material is subjected to permeation treatment on a dried specimen base material through the processes of softening, needle insertion, wing spreading, drying, permeation film forming and the like, the appearance characteristics and the form of the specimen are not changed, a non-polar protective layer is formed in a capillary structure of the dried specimen, the moisture resistance of the specimen is favorably improved, and the service life of the dried specimen is further prolonged.

Description

Lepidoptera insect dried specimen manufacturing method

Technical Field

The invention relates to a method for preparing a lepidoptera insect dried specimen.

Background

The existing dried specimen is mostly preserved by qualitative drying, and is preserved in a dry environment by adding a drying agent, a mildew preventive and the like, the environmental requirement is strict, a temperature and humidity control device is required to be matched, once the specimen is exposed in the air for a long time, the specimen is easy to damp and damage due to moisture absorption and the like, and the protection performance is low. Particularly, the rare and protected species specimens are often lost due to the influence on scientific value caused by improper preservation. The method also adopts preservation forms such as soaking, film covering and the like for supplement, but the above forms have the defects of inconvenient carrying, limitation on the entity volume of the specimen, incapability of directly touching for detailed observation and the like. Later, the epoxy resin method for insect specimens appears in Guangdong and the like, solves the problem of preservation of biological specimens, but the existing method has some defects, such as the larger surface area and the larger polishing difficulty of the specimens, the easier generation of bubbles in the manufacturing process, the color change of the specimens caused by temperature rise and the like, and the further popularization of the technology is limited.

Disclosure of Invention

The invention relates to a method for manufacturing a dried specimen of a lepidoptera insect, which has the following specific technical scheme that the method for manufacturing the dried specimen of the lepidoptera insect is characterized by comprising the following manufacturing steps:

(1) and (3) softening: putting the collected lepidoptera insect specimen into a softener for 2-3 days, and checking the softening degree so as to prevent the specimen from being too soft and scrapped due to too long softening time;

(2) needle insertion: inserting a needle slightly off-center from the center of a sternum plate in the lepidoptera insect specimen to reserve a complete dorsal midline;

(3) spreading wings;

(4) and (3) drying: placing the lepidoptera insect specimen into an oven, and standing for 24 hours at the temperature of 40 ℃;

(5) and (3) infiltration film forming: treating a specimen of a lepidopteran insect with a prepared penetrating film-forming material, comprising: spray pen atomizing spraying, titration absorption, soaking absorption and other treatment modes.

Further, the preparation of the permeable film-forming protective material comprises the following steps: A. sequentially adding an alkoxy silane mixture (5-8 wt%), a fluorine-containing compound (3-5 wt%) and organopolysiloxane (3-8 wt%) into a reaction kettle, and stirring to obtain a mixed solution; B. then adding silane coupling agent (0.5-2% by weight) into the mixed solution and stirring uniformly to form a premix; C. adding catalyst (0.5-2 wt.%) into the pre-mixture, fully reacting for 1-3 hr, and finally adding solvent (80-90 wt.%).

Furthermore, the infiltration film can also adopt spraying or soaking treatment.

Further, the permeable film-forming material is a mixed solution of fluorine-silicon polymer.

Has the advantages that: the dried specimen substrate is subjected to permeation treatment by the organic silicon and fluorine silicon permeation film-forming materials, the appearance characteristics and the form of the specimen are not changed, and a nonpolar protective layer is formed in the capillary structure of the dried specimen, so that the moisture resistance of the specimen is improved, and the service life of the dried specimen is prolonged.

Drawings

FIG. 1: schematic diagram of water drop contact angle;

FIG. 2: a change chart of water drops on the surface of the specimen;

FIG. 3: and (5) a change chart of water drops on the surface of the treated specimen.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention are described below clearly and completely, and it is obvious that the described embodiments are some, not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Specimen preparation and processing in the present invention:

for the lepidoptera insect specimens, for example, the pteria camphorata and the spodoptera littoralis can be selected as specimens, and in this embodiment, the dried specimens of the pteria camphorata are taken as an example, and the manufacturing process is as follows:

(1) collecting a Papilio pteris sample in the field, and putting the Papilio pteris sample into a softener for 2-3 days;

(2) inserting the needle slightly off center from the middle chest back plate to leave a complete dorsal midline;

(3) spreading the wings after the needle insertion, putting the wings into an oven, and standing for 24 hours at the temperature of 40 ℃;

(4) and (3) infiltration film forming: the prepared membrane protective material is used for treating the specimen, specifically, a burette is used for absorbing and permeating the membrane forming material to perform titration absorption treatment on each part of the Paenira cinnamomea sample, including the abdomen, the wings and the like, and after uniform absorption, the Paenira cinnamomea sample is naturally dried for 48 to 72 hours.

The wings of the lepidoptera adults are covered with a large amount of scales, the abdomen of the lepidoptera adults is rich in villi, the area of the lepidoptera adults is relatively small, but the absorption speed of the film-coated materials is high, and the problems that villi are stressed and collapsed, the scales are blown away and the like are easily caused by spraying with a spray can or a spray pen are solved, so that the method is preferentially adopted for treatment in a dropper titration mode.

Wherein, the process for preparing the permeable film-forming protective material comprises the following steps:

A. sequentially adding an alkoxy silane mixture (8 wt%), a fluorine-containing compound (4 wt%) and organopolysiloxane (6 wt%) into a reaction kettle, and stirring to obtain a mixed solution;

B. then adding a silane coupling agent (1.5 percent by weight) into the mixed solution and uniformly stirring to form a premix;

C. adding catalyst (0.5 wt%) into the pre-mixture, fully reacting for 1 hr, and finally adding solvent (80 wt%).

Has the advantages that:

as shown in fig. 1, the contact angle of a drop of water, which results from solid-liquid interface interactions, may spread onto a solid substrate when the drop is placed on a horizontal surface of the substrate, the contact angle value approaches zero if fully wetted or absorbed, and conversely, if wetted or absorbed partially, the resulting contact angle is balanced over the range of material surface energies, the drop attaches to the substrate surface and casts a shadow, and a projection screen micrometer uses optical magnification to project an image onto a screen for measurement to obtain an accurate contact angle value. The smaller the contact angle, the greater the wettability, absorption properties or surface energy of the substrate and the lower the ability to repel and repel moisture. In this example, the specimens before and after the coating treatment were measured by a water droplet contact angle measuring instrument (dropping accuracy 0.01. mu.l, cold light source, model SL 200B).

As shown in figure 2, the test is respectively carried out on the wings and the abdomen of the specimen, and the water drops are absorbed by the specimen after 10 minutes and completely permeate the specimen; as shown in fig. 3, after the treatment, there was almost no absorption of the water droplets, and the contact angle decreased from 136.71 degrees to 133.98 degrees.

TABLE 1 Change in water droplet on the surface of the feature before and after treatment

Because the lepidoptera wings are distributed with scale structures, the contact angle of the pure water medium on the surfaces of the wings is larger, and the angle change rate has no obvious difference. Thus, the moisture resistance of the abdomen is remarkably improved. The integral moisture resistance of the specimen is improved, so that the service life of the specimen is prolonged, and the preservation performance of the biological specimen in various conventional complex environments is improved.

Other variations and modifications will occur to those skilled in the art from the foregoing disclosure and teachings and are, nevertheless, within the scope of the appended claims.

Claims

1. A lepidoptera insect dried specimen preparation method is characterized by comprising the following preparation steps:

(3) spreading wings;

(5) and (3) infiltration film forming: and (3) carrying out titration absorption treatment on the lepidoptera insect specimen by using the prepared permeable film-forming material.

2. The method for preparing a lepidopteran insect dried specimen according to claim 1, wherein the preparation of the infiltration film-forming material comprises the steps of: A. sequentially adding an alkoxy silane mixture (5-8 wt%), a fluorine-containing compound (3-5 wt%) and organopolysiloxane (3-8 wt%) into a reaction kettle, and stirring to obtain a mixed solution; B. then adding silane coupling agent (0.5-2% by weight) into the mixed solution and stirring uniformly to form a premix; C. adding catalyst (0.5-2 wt.%) into the pre-mixture, fully reacting for 1-3 hr, and finally adding solvent (80-90 wt.%).

3. The method for preparing a dried specimen of lepidopteran insects according to claim 1, wherein said infiltration film is formed by spraying or dipping.

4. The method for preparing a lepidopteran insect dried specimen according to claim 1, wherein the infiltration film-forming material is a mixed solution of a fluorosilicone polymer.