CN101653113A - Method for manufacturing nontoxic specimen of marine organism - Google Patents
Method for manufacturing nontoxic specimen of marine organism Download PDFInfo
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- CN101653113A CN101653113A CN200910152492A CN200910152492A CN101653113A CN 101653113 A CN101653113 A CN 101653113A CN 200910152492 A CN200910152492 A CN 200910152492A CN 200910152492 A CN200910152492 A CN 200910152492A CN 101653113 A CN101653113 A CN 101653113A
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Abstract
The invention discloses a method for manufacturing a nontoxic specimen of a marine organism. A manufacturing process of the method comprises the following steps: pretreatments of specimen cleaning, freezing, sublimation drying, sterilizing, and the like; specimen maintenance; specimen coloring and restoration; specimen shaping; and protective film coating. The invention is characterized in that aphotocureable coating is used for the manufacture of a specimen for the first time, thereby overcoming the defect of using virulent coatings, and the photocureable coating is colorless liquid with good flow property, can be immediately cured under the illumination of light with specific wavelength and can be formed a dense film which almost cannot be seen by eyes; the film is tightly adhered on the surface of the specimen body so as to prevent the surface of the specimen body being contacted with air and prevent insects from encroaching the specimen body; meanwhile, the protective film also has the function of water proofing, and even water vapor cannot invade, thus the film has the function of preventing rot , mildew and deformation.
Description
Technical field:
The present invention relates to the biological sample technical field, introduce a kind of manufacturing technology of biological sample, specifically is a kind of preparation method of nontoxic specimen of marine organism.
Background technology:
Biological sample is a kind of capsule information that teaching and research are provided, but various organisms are because characteristic differences such as its inherent institutional framework, individual sizes, the method of its preparation of specimen also is not quite similar, and having nutrition and colloid institutional frameworks such as amounts of protein in the marine organism, the manufacturing technology of its sample is more taken twists and turns more than the preparation of specimen of insect, plant class.At present, the preparation method of more common marine organism specimen is:
The pre-treatment of sample: key step comprise select have complete, representative, typicalness marine organism as halobiontic making object, through the appearance impurity elimination, clean, freeze, lyophilization, behind the sterilization, remove its vegetative tissue structure, and the try one's best outward appearance of retains biological activity and the form of primary object.
The maintenance of sample: if when sample has damage or local shedding in the pre-treatment of making, repair, paste, place shady and cool place airing according to natural posture with writing brush brush coating water (natural plant gum).The sample that if mildew arranged or damage by worms, dip in 95% alcohol or 10% formalin solution is scrubbed with writing brush, do the back and brush mildew with writing brush.
The painted of sample answered very: finish the marine organism sample of making as stated above, some changes usually occur on appearance luster, for fear of distortion, keep the authenticity of organism sample, need carry out suitable reparation with pigment.
The typing of sample is handled: through finishing the marine organism specimen of making and keeping in repair according to the method described above, should put a week at shady and cool dry place, allow it return to natural form.
The surface coating of sample: preparation of specimen finish and airing after, must prevent chemicalss such as organism is rotted, goes mouldy, damages by worms, variable color, distortion in sample extexine coating.
Depositing of sample: after preparation of specimen was good, the village was placed in the transparent cabinet that is of moderate size with sample with mark, as glass cabinet, for some oversized samples, generally all was to open wide to place, and sticked poisonous caution label.
Because in the sterilization process of sample pre-treatment and the filming of sample surface, employed all is poisonous chemicals generally, as: the sterilization medicine is generally mercuric chloride alcohol, and the medicine of surface coating generally is the extremely malicious chemicals of a class such as white arsenic.These chemical residues poisonous or extremely poison are on the sample surface, and are careless slightly in carrying or putting on display, and cause staff or visit personnel that intoxication accident takes place easily.
Summary of the invention:
The technical problem to be solved in the present invention is at the defective that exists in the above-mentioned prior art, to provide a kind of preparation method that can isolate the nontoxic specimen of marine organism of sample surface residual hazard.
Technical solution of the present invention is:
A kind of preparation method of nontoxic specimen of marine organism, its manufacturing process comprises the steps:
1, the pre-treatment of sample: key step comprise select have complete, representative, typicalness marine organism as halobiontic making object, through the appearance impurity elimination, clean, freeze, lyophilization, behind the sterilization, remove its vegetative tissue structure, and keep the mode of appearance of organism as far as possible.
2, the maintenance of sample: if when sample has damage or local shedding in the pre-treatment of making, repair with writing brush brush coating water (natural plant gum), paste according to natural posture, the sample that if mildew is arranged or damage by worms, dip in 95% alcohol or 10% formalin solution is scrubbed with writing brush, do the back and brush mildew with writing brush; Place shady and cool place airing.
3, sample is painted multiple true: finish the marine organism sample of making as stated above, some changes usually occur on appearance luster, for fear of distortion, keep the authenticity of organism sample, need carry out the appropriate colouring reparation with pigment.
4, the typing of sample is handled: through finishing the marine organism specimen of making and keeping in repair according to the method described above, should put a week at shady and cool dry place, the processing that formalizes makes it to return to natural form.
5, the coating of diaphragm: the sample processing that formalizes after making it to return to natural form, smears outer surface at sample with the modulated photocureable coating that makes; Also can photocureable coating be smeared outer surface at sample with spraying or the mode of showering; Smear finish after, it is moved to carries out ultraviolet irradiation under the ultraviolet lamp, make it form cured film and be compound in the sample surface; If necessary, can do coating for the second time on the sample surface, form thicker composite membrane behind the irradiation through the second time.
Compared with prior art, the present invention has the following advantages:
The innovative characteristics of this method is that to use photocureable coating, photocureable coating first in preparation of specimen be a kind of colourless, good liquid of mobile performance, penetrates down in the illumination of specific wavelength and solidifies immediately, forms the film that the eyes of one deck densification almost be can't see.This film is attached on the surface of sample body tightly, makes the sample surface avoid contacting with air, prevents insect pest infestation sample body; Simultaneously, this layer diaphragm also has the function of waterproof, even steam can not invade, thus can play anti-ly rot, anti-ly go mouldy, distortion-free effect.
This photocureable coating that uses forms the stable chemical performance of fine and close film, and is nontoxic, thus the sample that uses this method to make, the drawback of having got rid of traditional use severe toxicity coating.
Embodiment:
Below the invention will be further described:
A kind of preparation method of nontoxic specimen of marine organism, its concrete steps are as follows:
1, the pre-treatment of sample: key step comprise select have complete, representative, typicalness marine organism as halobiontic making object, through the appearance impurity elimination, clean, freeze, lyophilization, behind the sterilization, remove its trophic structure, as far as possible the outward appearance of retains biological activity and the form of primary object.Require its water content in the 1-5.5% standard.Wherein, the sterilization process generally is to adopt two kinds of methods, the one,, with sample body and the container that fills sterilization medicine carbon disulphide, put into the desinsection case simultaneously, unpack after two days, poison gas is dispersed, take out sample; The 2nd,, the sample of the paper of not appearing on the stage is put into 0.5% mercuric chloride alcohol (industrial 75%) solution soak the sterilization that carries out disinfection.
2, the maintenance of sample: if when sample has damage or local shedding in the pre-treatment of making, repair, generally select natural plant gum for use, paste, place shady and cool place airing according to the natural posture of sample thing with writing brush brush coating water; Can use the vinyl acetate glue when fracture place occurring, stick hinge again; If any the mildew or the sample of damaging by worms, available writing brush dips in 95% alcohol or 10% formalin solution is scrubbed, and treats that it does the back and brush mildew with writing brush; Will changing of platform paper and the breakage of lid paper is new.
3, sample is painted multiple true: finish the marine organism sample of making as stated above, some changes usually occur on appearance luster, for fear of distortion, keep the authenticity of organism sample, need carry out suitable reparation with pigment.Select to use inorganic pigment during reparation as far as possible; Finishing painted multiple true time, just can use a spot of watercolors.
4, the typing of sample is handled: through finishing the marine organism specimen of making and keeping in repair according to the method described above, should put a week at shady and cool dry place, allow it return to natural form, should not be placed with sunlight direct irradiation, high temperature or the place below 0 ℃, dry in the shade a few days, generally with a week for well, the processing that formalizes makes it to return to natural form.
5, the coating of diaphragm: the sample processing that formalizes after making it to return to natural form, smears outer surface at sample with the modulated photocureable coating that makes; Also can photocureable coating be smeared outer surface at sample with spraying or the mode of showering; Smear finish after, it is moved to carries out ultraviolet irradiation under the ultraviolet lamp, make it form cured film and be compound in the sample surface; If necessary, can do coating for the second time on the sample surface, form thicker composite membrane behind the irradiation through the second time.
Photocureable coating mainly just is made up of photosensitive resin, sensitising agent (light trigger) and thinner.Photosensitive resin generally is the low-molecular-weight resin that has unsaturated bond, as unsaturated polyester (UP), acrylic oligomers; Sensitising agent is easily to absorb the compound that ultraviolet light produces living radical, as benzophenone, styrax alkyl ether; The main effect of thinner is to reduce dope viscosity, also participates in film-forming simultaneously, is reactive diluent, as styrene, acrylate etc.
The photocureable coating that present embodiment is selected for use is an Epocryl.
Known to the inventor, described Epocryl, be by tetrabromobisphenol A, epoxy diacrylate,, trimethylolpropane triacrylate, hexanediyl ester, methyl methacrylate/ethyl acrylate copolymer, 2-chlorinated thio xanthone, triethylamine, in having the container of agitator, fully mix, mix with rutile titanium dioxide again, behind ball mill grinding, make mixture, be modulated into coating again with this mixture.Described Epocryl at present, is domesticly produced, can buy on market.
Claims (2)
1, a kind of preparation method of nontoxic specimen of marine organism, its manufacturing process comprises the steps:
(1), the pre-treatment of sample: key step comprise select have complete, representative, typicalness marine organism as making object, through the appearance impurity elimination, clean, freeze, lyophilization, behind the sterilization, remove its vegetative tissue structure, and keep the mode of appearance of organism as far as possible;
(2), the maintenance of sample: if when sample has damage or local shedding in the pre-treatment of making, with writing brush brush coating water, generally use the natural plant gum place under repair, natural posture according to organism pastes, as if mildew being arranged or damaging by worms, dip in 95% alcohol or 10% formalin solution is scrubbed with writing brush, do the back and brush mildew, place shady and cool place airing with writing brush;
(3), sample is painted multiple true: finish the marine organism sample of making as stated above, some changes usually occur on appearance luster, for fear of distortion, keep the authenticity of organism sample, need carry out the appropriate colouring reparation with pigment;
(4), the typing of sample is handled: through finishing the marine organism specimen of making and keeping in repair according to the method described above, should put a week at shady and cool dry place, the processing that formalizes makes it to return to natural form;
It is characterized in that:
(5), the coating of diaphragm: the sample processing that formalizes after making it to return to natural form, smears outer surface at sample with the modulated photocureable coating that makes; Also can photocureable coating be smeared outer surface at sample with spraying or the mode of showering; Smear finish after, it is moved to carries out ultraviolet irradiation under the ultraviolet lamp, make it form cured film and be compound in the sample surface; If necessary, can do coating for the second time on the sample surface, form thicker composite membrane behind the irradiation through the second time.
2, the preparation method of nontoxic specimen of marine organism as claimed in claim 1 is characterized in that: described photocureable coating is an Epocryl.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910152492A CN101653113A (en) | 2009-09-10 | 2009-09-10 | Method for manufacturing nontoxic specimen of marine organism |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910152492A CN101653113A (en) | 2009-09-10 | 2009-09-10 | Method for manufacturing nontoxic specimen of marine organism |
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| CN101653113A true CN101653113A (en) | 2010-02-24 |
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| CN200910152492A Pending CN101653113A (en) | 2009-09-10 | 2009-09-10 | Method for manufacturing nontoxic specimen of marine organism |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102415369A (en) * | 2011-08-03 | 2012-04-18 | 刘卉秋 | Eupatorium odoratum standard sample and preparation method thereof |
| TWI511661B (en) * | 2014-06-27 | 2015-12-11 | Univ Nat Pingtung Sci & Tech | Manufacturing method of a plant specimen |
| CN108130279A (en) * | 2018-01-08 | 2018-06-08 | 浙江海洋大学 | A kind of mold-proof method of marine organism specimen |
| CN109085028A (en) * | 2018-06-07 | 2018-12-25 | 西南大学 | The production method that citrus leaves disease solidifies sample |
| CN110402905A (en) * | 2018-04-28 | 2019-11-05 | 张家港市德仁科教仪器设备有限公司 | A kind of insect trap |
| CN113115762A (en) * | 2019-12-30 | 2021-07-16 | 上海市林业总站 | Method for preparing dried specimen of coleoptera insect |
| CN113115763A (en) * | 2019-12-30 | 2021-07-16 | 上海市林业总站 | Lepidoptera insect dried specimen manufacturing method |
-
2009
- 2009-09-10 CN CN200910152492A patent/CN101653113A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102415369A (en) * | 2011-08-03 | 2012-04-18 | 刘卉秋 | Eupatorium odoratum standard sample and preparation method thereof |
| TWI511661B (en) * | 2014-06-27 | 2015-12-11 | Univ Nat Pingtung Sci & Tech | Manufacturing method of a plant specimen |
| CN108130279A (en) * | 2018-01-08 | 2018-06-08 | 浙江海洋大学 | A kind of mold-proof method of marine organism specimen |
| CN110402905A (en) * | 2018-04-28 | 2019-11-05 | 张家港市德仁科教仪器设备有限公司 | A kind of insect trap |
| CN109085028A (en) * | 2018-06-07 | 2018-12-25 | 西南大学 | The production method that citrus leaves disease solidifies sample |
| CN113115762A (en) * | 2019-12-30 | 2021-07-16 | 上海市林业总站 | Method for preparing dried specimen of coleoptera insect |
| CN113115763A (en) * | 2019-12-30 | 2021-07-16 | 上海市林业总站 | Lepidoptera insect dried specimen manufacturing method |
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Application publication date: 20100224 |