CN108925547A - A kind of thrips class insect permanent slide Slide processing - Google Patents
A kind of thrips class insect permanent slide Slide processing Download PDFInfo
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- CN108925547A CN108925547A CN201811086704.XA CN201811086704A CN108925547A CN 108925547 A CN108925547 A CN 108925547A CN 201811086704 A CN201811086704 A CN 201811086704A CN 108925547 A CN108925547 A CN 108925547A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
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- G—PHYSICS
- G09—EDUCATION; CRYPTOGRAPHY; DISPLAY; ADVERTISING; SEALS
- G09B—EDUCATIONAL OR DEMONSTRATION APPLIANCES; APPLIANCES FOR TEACHING, OR COMMUNICATING WITH, THE BLIND, DEAF OR MUTE; MODELS; PLANETARIA; GLOBES; MAPS; DIAGRAMS
- G09B23/00—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes
- G09B23/36—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes for zoology
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- Animal Behavior & Ethology (AREA)
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Abstract
The invention discloses a kind of thrips class insect permanent slide Slide processings, and this method includes: (1) it decolourizes: thrips class insect being taken out from alcoholic solution, is put into lye, soaking at room temperature decoloration；(2) it is dehydrated: thrips class insect is taken out from the lye, be placed in distilled water and the intracorporal content of insect is allowed to be discharged, impregnated step by step with the alcoholic solution of increasing concen-trations to be dehydrated；(3) transparent: by thrips class insect be sucked out be placed on filter paper, allow insect surfaces alcohol natural air drying to filter paper on there is no solvent, insect is impregnated in dimethylbenzene；(4) mounting: dripping neutral gum or canada balsam on glass slide, insect be placed on the neutral gum or canada balsam, each position of insect body is set, and covered is dried, and obtains permanent slide sample.The done sample transparency of method of the invention is high, and the resting period is long, short effect the time required to production sample.
Description
Technical field
The present invention relates to insect specimen production methods, and in particular to a kind of thrips class insect permanent slide preparation of specimen side
Method.
Background technique
Thrips is an insect assembly close with human relation, many kinds of, and quantity is abundant.Some kinds of thrips
Class happiness is inhabited between flower, has stronger pollination ability；Many phytophagous types are important crop pest, a small amount of predatism and
Bacterium feeding habits type can be used as nature enemy in amount.
Thrips individual is smaller, most of long 0.5-5mm of type body, therefore Clear & Transparent slide sample is thrip fuana standard
The precondition really identified.Thrips permanent slide preparation of specimen generally requires by decoloration, dehydration, transparent, mounting, respectively
The control of reagent needed for a step and time are an important factor for influencing sample superiority and inferiority.
Summary of the invention
The object of the present invention is to provide a kind of thrips class insect permanent slide Slide processing, this method is able to solve existing
Have permanent slide preparation of specimen low-concentration alkali liquor decoloration be not thorough with dehydration of alcohol complex steps, time-consuming the problem of, mark
This transparency is high, and the resting period is long, short effect the time required to production sample.
In order to achieve the above object, the present invention provides a kind of thrips class insect permanent slide Slide processing, the party
Method includes:
(1) it decolourizes: thrips class insect being taken out from alcoholic solution, is put into lye, soaking at room temperature decoloration；
(2) it is dehydrated: thrips class insect is taken out from the lye, be placed in distilled water and the intracorporal content of insect is allowed to arrange
Out, it is impregnated step by step with the alcoholic solution of increasing concen-trations to be dehydrated；
(3) transparent: thrips class insect being sucked out and is placed on filter paper, is allowed on the alcohol natural air drying to filter paper of insect surfaces
There is no solvent, insect is impregnated in dimethylbenzene；
(4) mounting: dripping neutral gum or canada balsam on glass slide, and insect is placed in the neutral gum or is added and is taken
On big natural gum, each position of insect body is set, covered is dried, and permanent slide sample is obtained.
Preferably, in step (1), the lye includes: NaOH solution.
Preferably, the lye is 6%NaOH solution.
Preferably, in step (1), soaking at room temperature bleaching time are as follows: 8~for 24 hours or 4~6h, the time is according to insect
Shade determines.
Preferably, in step (2), the thrips class insect is placed in 2h in distilled water.
Preferably, in step (2), the alcoholic solution concentration is gradually incremented by 30%~100%.
Preferably, the alcoholic solution concentration is 30%, 60%, 85%, 95%, 100%.
Preferably, 5~10min is impregnated respectively under 30%, 60%, 85%, 95% concentration of alcoholic solution,
30min is impregnated under 100% concentration.
Preferably, in step (3), 30min is impregnated in the dimethylbenzene.
Preferably, in step (3), under anatomical lens, with insect needle instigate each position of insect body, covered,
Naturally dry obtains permanent slide sample.
Thrips class insect permanent slide Slide processing of the invention, it is low dense to solve existing permanent slide preparation of specimen
Degree lye decoloration be not thorough with dehydration of alcohol complex steps, time-consuming problem, have the advantage that
The done sample transparency of method of the invention is high, and the resting period is long, and dehydration is only provided with 5 alcohol gradients, saves
Time.Moreover, when carrying out alcohol natural air drying, the time on filter paper is not easy to hold, and the time is long in previous method
Cause have bubble inside polypide, the time is short, and there is alcohol on polypide surface and sample is muddy, and method of the invention is specified and filtered
Time on paper, being subject to does not have " bright water ".
Specific embodiment
The following further describes the technical solution of the present invention with reference to embodiments.
A kind of thrips class insect permanent slide Slide processing, this method includes:
(1) it decolourizes: thrips class insect being taken out from alcoholic solution, is put into lye, soaking at room temperature decoloration；
(2) it is dehydrated: thrips class insect is taken out from lye, be placed in distilled water and the intracorporal content of insect is allowed to be discharged,
It is impregnated step by step with the alcoholic solution of increasing concen-trations to be dehydrated；
(3) transparent: thrips class insect being sucked out and is placed on filter paper, is allowed on the alcohol natural air drying to filter paper of insect surfaces
There is no solvent, that is, there is no " bright water ", insect is impregnated in dimethylbenzene；
(4) mounting: dripping neutral gum or canada balsam on glass slide, and insect is placed in neutral gum or Canada sets
On glue, each position of insect body is set, covered is dried, and permanent slide sample is obtained.
The effect of neutral gum or canada balsam is that glass slide and coverslip are bonded together, for retaining life for a long time
Object histotomy is sealed up for safekeeping.
Further, in step (1), lye includes: NaOH solution.Further, lye is 6%NaOH solution,
The concentration is most appropriate, and decoloration is not thorough when concentration is too low, and when excessive concentration will cause polypide deformation.
Further, in step (1), soaking at room temperature bleaching time is 8~for 24 hours or 4~6h, if bleaching time is too short
It will cause decoloration to be not thorough, bleaching time is long to will also result in polypide deformation.Insect color depth bleaching time is long, be 8~for 24 hours；Elder brother
Worm bleaching time of light color is short, is 4~6h.
Further, in step (2), thrips class insect is placed in 2h in distilled water, so that insect content is sufficiently discharged,
Otherwise it will affect the clarity of slide observation.
Further, in step (2), alcoholic solution concentration is gradually incremented by 30%~100%.Specifically, alcohol is molten
Liquid concentration is 30%, 60%, 85%, 95%, 100%.More specifically, dense in alcoholic solution 30%, 60%, 85%, 95%
5~10min is impregnated under degree respectively, polypide the greater soaking time is slightly long, impregnates 30min under 100% concentration.Alcohol concentration
Selection is finally determined by largely practicing, and gradient can waste time too much, can cause polypide torsional deformation very little, and right
It to be dehydrated since the alcohol of low concentration in the tissue for being easy crisp, otherwise tissue, which is easy to become fragile, is hardened.
Further, in step (3), 30min is impregnated in dimethylbenzene, dimethylbenzene is as clarifier.
Further, in step (3), under anatomical lens, each position of insect body is instigateeed with insect needle, close the lid glass
Piece, naturally dry obtain permanent slide sample.
Embodiment 1
(1) it decolourizes: 20 or so thrips class insects is directly placed into 6%NaOH solution from choosing in alcoholic solution,
It is placed at room temperature for 8~24 hours；Depending on body size and body colour, individual big, color depth soaking time will be grown soaking time；It is few
The sample of number very slight color can be without decoloration Direct Dehydration；
(2) it is dehydrated: thrips class insect being taken out from lye, is placed in 2 hours in distilled water, make body content natural
Discharge, is then dehydrated step by step with the alcoholic solution of various concentration, i.e., is impregnated respectively with 30%, 60%, 85%, 95% alcoholic solution
5~10 minutes, then sample is transferred in 100% alcohol, is placed 30 minutes；
(3) transparent: thrips class insect being sucked out with suction pipe and is spat on ordinary filter paper, allows the alcohol natural wind on polypide surface
Dry, the time should not too long, and being subject on filter paper does not have " bright water ", otherwise made of have bubble in slide polypide, then use elder brother
Worm needle gently instigates worm in dimethylbenzene, impregnates 30 minutes；
(4) mounting: dripping canada balsam in glass slide center, thrips one be placed on glue, under anatomical lens, uses
Insect needle carefully instigates each position of polypide, gently covered, after slide makes, is placed on plate, naturally dry,
The information such as acquisition time, collecting location, acquisition people are indicated on slide, obtain permanent slide sample.
In conclusion the done sample transparency of thrips class insect permanent slide Slide processing of the invention is high, storage
It is of long duration, short effect the time required to production sample.
It is discussed in detail although the contents of the present invention have passed through above preferred embodiment, but it should be appreciated that above-mentioned
Description is not considered as limitation of the present invention.After those skilled in the art have read above content, for of the invention
A variety of modifications and substitutions all will be apparent.Therefore, protection scope of the present invention should be limited to the appended claims.
Claims (10)
1. a kind of thrips class insect permanent slide Slide processing, which is characterized in that this method includes:
(1) it decolourizes: thrips class insect being taken out from alcoholic solution, is put into lye, soaking at room temperature decoloration；
(2) it is dehydrated: thrips class insect is taken out from the lye, be placed in distilled water and the intracorporal content of insect is allowed to be discharged,
It is impregnated step by step with the alcoholic solution of increasing concen-trations to be dehydrated；
(3) transparent: by thrips class insect be sucked out be placed on filter paper, allow insect surfaces alcohol natural air drying to filter paper on do not have
Solvent impregnates insect in dimethylbenzene；
(4) mounting: dripping neutral gum or canada balsam on glass slide, and insect is placed in the neutral gum or Canada sets
On glue, each position of insect body is set, covered is dried, and permanent slide sample is obtained.
2. thrips class insect permanent slide Slide processing according to claim 1, which is characterized in that in step (1)
In, the lye includes: NaOH solution.
3. thrips class insect permanent slide Slide processing according to claim 2, which is characterized in that the lye is
6%NaOH solution.
4. thrips class insect permanent slide Slide processing according to claim 3, which is characterized in that in step (1)
In, soaking at room temperature bleaching time is 4~6h or 8~for 24 hours.
5. thrips class insect permanent slide Slide processing according to claim 1, which is characterized in that in step (2)
In, the thrips class insect is placed in 2h in distilled water.
6. thrips class insect permanent slide Slide processing according to claim 1, which is characterized in that in step (2)
In, the alcoholic solution concentration is gradually incremented by 30%~100%.
7. thrips class insect permanent slide Slide processing according to claim 6, which is characterized in that the alcohol is molten
Liquid concentration is 30%, 60%, 85%, 95%, 100%.
8. thrips class insect permanent slide Slide processing according to claim 7, which is characterized in that in the alcohol
5~10min is impregnated respectively under 30%, 60%, 85%, 95% concentration of solution, impregnates 30min under 100% concentration.
9. thrips class insect permanent slide Slide processing according to claim 1, which is characterized in that in step (3)
In, 30min is impregnated in the dimethylbenzene.
10. thrips class insect permanent slide Slide processing according to claim 1, which is characterized in that in step (3)
In, under anatomical lens, each position of insect body, covered are instigateeed with insect needle, naturally dry obtains permanent slide mark
This.
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| CN201811086704.XA CN108925547A (en) | 2018-09-18 | 2018-09-18 | A kind of thrips class insect permanent slide Slide processing |
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| CN201811086704.XA CN108925547A (en) | 2018-09-18 | 2018-09-18 | A kind of thrips class insect permanent slide Slide processing |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109371016A (en) * | 2018-12-18 | 2019-02-22 | 吉林大学 | The method of non-destructive high efficiency extraction DNA from single head thrips class insect specimen |
| CN109827832A (en) * | 2019-01-17 | 2019-05-31 | 河南师范大学 | The production method of branch angular motion object Permanent slide |
| CN112889807A (en) * | 2021-02-09 | 2021-06-04 | 陕西理工大学 | Method for manufacturing permanent microscopic slide specimen of tiny insect |
| CN113533329A (en) * | 2021-07-12 | 2021-10-22 | 公安部物证鉴定中心 | Fiber comparison sheet for forensic science polarized light microscope test and preparation method thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109371016A (en) * | 2018-12-18 | 2019-02-22 | 吉林大学 | The method of non-destructive high efficiency extraction DNA from single head thrips class insect specimen |
| CN109827832A (en) * | 2019-01-17 | 2019-05-31 | 河南师范大学 | The production method of branch angular motion object Permanent slide |
| CN112889807A (en) * | 2021-02-09 | 2021-06-04 | 陕西理工大学 | Method for manufacturing permanent microscopic slide specimen of tiny insect |
| CN113533329A (en) * | 2021-07-12 | 2021-10-22 | 公安部物证鉴定中心 | Fiber comparison sheet for forensic science polarized light microscope test and preparation method thereof |
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