CN204177654U - For the experimental provision that the sampling of biological in-situ printingout method and trace are dyeed - Google Patents
For the experimental provision that the sampling of biological in-situ printingout method and trace are dyeed Download PDFInfo
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- CN204177654U CN204177654U CN201420676756.3U CN201420676756U CN204177654U CN 204177654 U CN204177654 U CN 204177654U CN 201420676756 U CN201420676756 U CN 201420676756U CN 204177654 U CN204177654 U CN 204177654U
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Abstract
The utility model relates to a kind of experiment equipment for biological printingout method microsection, it is characterized in that: comprise printingout, press from both sides for the printingout of gripping printingout, for holding the printingout box of printingout; Described printingout is T-shaped, and the wide part in described printingout upper end is mark zone portion, and the narrow part in lower end is sample area portion; Described printingout box inwall is provided with the stopper slot in the mark zone portion of restriction printingout.Also comprise the base for piling up printingout box, the bottom of described base and printingout box is provided with the structure be used in conjunction with each other.Directly printingout can be clamped by printingout by the utility model, be placed on cultivated nutrient culture media, glue and get culture, again the sticky printingout getting culture is placed in printingout box, carry out dyeing, drying, the operation steps such as preservation, printingout is kept in box after completing dyeing and drying, and when microscopic examination, taking-up liquid mounting glue is affixed on microslide, puts into former box and preserve for a long time after microscopic examination after needing the printingout preserved to come unstuck.
Description
Technical field
The utility model belongs to biological Instrument and equipments field, especially relates to the experimental provision for the sampling of biological in-situ printingout method and trace dyeing.
Background technology
Optical microscope tabletting technology is technology biomaterial being made the thin slice being suitable for observing under an optical microscope.Although the simple method of fresh biomaterial makes interim film-making also observable, in order to preserve in order to later observation, often Permanent production need be made by certain step.
First microsection will keep the native state of biomaterial as far as possible, avoids image, distortion and distortion, and therefore must fix biomaterial process; Film-making must be thin and transparent, could imaging under an optical microscope, except being thinly sliced by material or making it except dispersion by light pressure or other means, also needs to adopt additive method to make its transparent and dye, to observe the details of structure better.Need long-term film-making of preserving, also should carry out dewatering and sealing.
Microsection method generally comprises the large class of microtomy, integral mounting method, smear method and pressed disc method 4.
The slice thickness of microtomy optical microscope is between 2 ~ 25 microns, and the section of general animals and plants material is suitable with thick 10 microns.Microtomy is different according to the difference of embedding medium.The most frequently used is paraffin method, and it is applicable to general biomaterial.Celloidin microtomy, biomaterial section after celloidin embedding.Be applicable to material hard especially or particularly soft and bulky material (brain as complete).Frozen sections, cuts into slices biomaterial after freezing solidification in certain medium.Be applicable to research living specimen or do histochemical research.Ethylene Glycol Methyl acrylate method (being called for short GMA method), is applicable to making 1 ~ 3 micron of slab.Paraffin method comprises fixing, embedding, section, dyeing, dehydration and the step such as sealing.Key is biomaterial paraffin embedding, take paraffin as holder, the biomaterial be immersed in wax stone is cut into desirable thin slice.Operating process is: fixing → washing → from low concentration step by step to alcohol in high concentration dehydration → dimethylbenzene transparent → waxdip → embedding → section → paster → dimethylbenzene dewaxes → step by step from high concentration to low-concentration ethanol process, be finally transitioned into the dehydration → dimethylbenzene from low concentration to alcohol in high concentration of water → dyeing → step by step transparent → resin glue sealing.Basic step is wherein all identical in various tabletting technology.
Integral mounting method is used for the whole mount preparation method of the organ of unicellular, micro-organisms or dispersion.This method also needs through fixing, dyeing, dehydration, transparent and each step of sealing is poly-.Fuchsin commonly used by material.The single decoration methods such as Dai Shi haematine, but also can redye.In integral mounting method, conventional wintergreen, caryophyllus oil one class essential oil are as clarifier, to replace dimethylbenzene, can avoid sample embrittlement like this, thicker sample when sealing, at cover glass underlay with the cullet of suitable size or glass fiber in order to avoid damage by pressure.
Smear method is coated on the biological sample being easy to disperse the flaking method on microslide.The Permanent production of this method need through fixing, dyeing, and dehydration waits step poly-, is applicable to the material that bacterium, blood, pollen mother cell and sperm etc. are carefully small and scattered.Blood smear is an example.
Pressed disc method by natural, be easy to the tissue that disperses or be easy to the tissue that disperses after treatment, as the sperm mother cell of animal, the salivary gland of fruit bat, the pollen mother cell of plant and root-tip cells etc. are placed on microslide, again and add cover glass, firmly crush tissue, make cell or intracellular structure sprawl into the flaking method of one deck.Pressed disc method is usually used in observing chromosome, usually with the dyeing of aceto-camine, cudbear and carbolfuchsin.
Tradition microtomy, integral mounting method, smear method and the pressed disc method common feature to the biological sampling such as bacterium chooses (receipts) to get macroscopic culture (bacterium colony or bacteria suspension) and carry out film-making, respectively has technical defect and deficiency by oneself.
First, the common feature of the culture of microorganism flaking method such as Conventional slide method collects bacterium with oese picking naked eyes visible colonies or hydro-extractor bacteria suspension to carry out film-making.
In oese picking colony and the physiological saline on slide in the process such as uniform application bacterium, easily destroy virgin state or the structure of bacterial growth, even cause bacteria lysis, such as, as L-type bacterium.Due to L-type bacteria cell wall defect, the easy dilatational strain of cell, therefore and is easily destroyed, thus makes in the past about L-type biology of bacteria characteristic research and clinical detection all comparatively difficulty.But L-form of bacterium variation is the biological nature of most of bacterium.Research shows in clinical bacteria infectious diseases, and L-form of bacterium variation phenomenon is very general, and clinical being easy to is failed to pinpoint a disease in diagnosis.
In addition, Conventional slide colouring method: usually adopt the manual dye liquor that drips to cover bacterium.In room temperature, in higher or air oxygen detrition situation, dye liquor easily evaporates dry, and observing to supplement will affect Color not in time, causes false positive or False negative error result.Further, in the specific stains such as bacterium histochemical staining or immune molecule group, classic method reagent is easily wasted, and causes cost high, not easily promotes the use of.
Prior art mainly completes microsection by microslide and cover glass and other experiment equipments, as mentioned above, film-making and preserve technological process numerous and diverse, compared with prior art, the utility model facilitates human users and management, effectively prevent cross pollution between sample, improve biological safety protection and source book management, and the mutual relationship (modes of reproduction) between bacterial growth form original appearance and non-form can be obtained by equipment of the present utility model and mutually distinguish, to obtain experimental result more accurately, can at utmost in-situ sampling, and than classic method more Rapid Detection pathogen, for the more effective diagnoses and treatment of patient is offered help.
Summary of the invention
For the problems referred to above, the technical problems to be solved in the utility model is to provide a kind of human users of convenience and management, can obtain the experiment equipment for biological in-situ printingout method microsection of mutual relationship (modes of reproduction) between bacterial growth form original appearance and different shape and difference mutually.
For achieving the above object, the utility model adopts following technical scheme: for the experimental provision of the sampling of biological in-situ printingout method and trace dyeing, it is characterized in that: comprise printingout, press from both sides for the printingout of gripping printingout, the preserving and the printingout box of dyeing function for holding having of printingout of one end open; Described printingout is T-shaped, and the wide part in described printingout upper end is mark zone portion, and the narrow part in lower end is sample area portion; Described printingout box inwall is provided with the stopper slot in the mark zone portion of restriction printingout.Printingout of the present utility model is PET optical film material, has the features such as heatproof, acid and alkali-resistance and pliability be better, is easy to print the culture got in double dish; Printingout box can be selected from the poly material of heatproof and make, and also can be selected from other materials and make.Heatproof polythene material can higher temperature resistant, and pliability is better, not easily broken, be easy to the culture got in double dish, and heatproof tygon also goes for other experimental procedures such as dyeing, decolouring, washing.Printingout, by arranging mark zone portion, can be numbered it by marking pen thereon, and arranges wider upper end, and can be clamped by the printingout that the utility model is special, printingout of taking is very convenient.Bottom, sample area is sticky part of getting culture, clamps printingout by printingout, sample area portion is directly covered in print in double dish and gets.Sampling process of the present utility model operates in the sterile biological safety cabinet of the cleaning of Microbiological Lab, adopting non-glass materials may injure directly avoiding operator, reducing the risk that bio-safety accident occurs.Printingout box, can by the restriction of the mark zone portion of printingout wherein by arranging stopper slot, and when being placed in one by printingout, sampling face and the another side relative with sampling face in sample area portion all do not contact with the box wall of printingout box.Sequence number can be set in mark zone portion during manufacture, conveniently test use, avoid fastening one person's story upon another person, also make a mark thereon to use by marker pen.
Preferably, described printingout folder comprises the clamping part that handle and elastomeric material are made; Described clamping part is provided with the groove for clamping printingout mark zone portion.Described clamping part is made up of elastomeric material because elastomeric material has certain elasticity, take printingout time printingout mark zone portion blocked by the groove of clamping part.The length direction of clamping part along the mark zone portion of printingout can be released parallel for clamping part when printingout being positioned in printingout box.In order to the clamping effect obtained also can arrange one-time formed tooth engaged in the groove of clamping part.
Described printingout folder is tweezers.Printingout of taking not only can be pressed from both sides by above-mentioned printingout, also can be completed by conventional tweezers.
Described printingout box also comprises the lid for covering printingout box.Ordinary stain or in order to preserve printingout, the time of needs is longer, in order to the dyeing that reached or preservation effect, can arrange lid and be sealed, also prevent the interference of other microorganisms in operation steps simultaneously.
Described lid can be provided separately with printingout box also can by being connected to the opening part of printingout box during compression molding.
Described printingout box inside is ovallized column chamber, and described ovallized column chamber constitutes the stopper slot in the mark zone portion of described restriction printingout at the two ends of the major axis of its oval cross section.By arranging cylindroid chamber, can stopper slot being set, simple process, and relatively saving staining reagent.
Described experiment equipment also comprises the base for piling up printingout box, and the bottom of described base and printingout box is provided with the structure be used in conjunction with each other.When an experiment has multiple sample, the base last time can pile up multiple printingout box, operates standard more, and handled easily manages.Experimentally need, usually can pile up 10 printingout boxes or more.
Preferably, the bottom of described printingout box is provided with slot or chuck, and described base is provided with the chuck or slot that are used in conjunction with each other with described printingout box and jointly forms with the slot of printingout box or chuck the structure piling up printingout box.
Preferably, the bottom of described printingout box is provided with projection or groove, and described base is provided with the groove that is used in conjunction with each other with described printingout box or protruding and jointly form with the projection of printingout box or groove the structure piling up printingout box.Described structure can be spheroidal, column or other can form the shape of structure.
Further, described base is provided with the baffle plate preventing printingout box from rotating for axle with described structure.
Accompanying drawing explanation
Fig. 1 is the structural representation of the utility model printingout;
Fig. 2 is the structural representation that the utility model has the printingout folder of folder rod and clamping part;
Fig. 3 is the structural representation of Fig. 1 and Fig. 2 when being combined;
Fig. 4 is the structural representation of the lid of embodiment 1,2,3 and 4;
Fig. 5 is the structural representation of embodiment 1 base;
Fig. 6 is the structural representation of embodiment 1 printingout box;
Fig. 7 is the schematic diagram of embodiment 1 when being contained in printingout box by printingout;
Fig. 8 is the structural representation that embodiment 1 piles up the printingout box filling printingout;
Fig. 9 is the structural representation under the printingout box of embodiment 2 and lid connection status;
Figure 10 is the structural representation of embodiment 2 base;
Figure 11 is the structural representation that embodiment 2 piles up the printingout box filling printingout;
Figure 12 is the structural representation of the printingout box of embodiment 3;
Figure 13 is the structural representation of embodiment 3 base;
Figure 14 is the inverted structural representation of printingout box of embodiment 4;
Figure 15 is the structural representation of embodiment 4 base;
Figure 16 is the structural representation that embodiment 4 piles up the printingout box filling printingout;
Figure 17 is the structural representation of the printingout box of embodiment 5;
Figure 18 is that Conventional slide method is for the cellular morphology figure under 1000 times of acid-fast stain optical microscopes of L-type bacterium;
Figure 19 is that the utility model is for the cellular morphology figure under 1000 times of acid-fast stain optical microscopes of L-type bacterium;
Figure 20 is that the utility model cultivates 1000 times of acid-fast stain optical microscope figure of 1 week for Sputum L-J method;
Figure 21 is that the utility model cultivates 1000 times of acid-fast stain optical microscope figure of 1 week for phlegm L-type tulase L-J method;
Figure 22 is the partial enlarged drawing in A portion in the middle of Figure 21;
In figure: 1, printingout; 2, printingout box; 3, mark zone portion; 4, sample area portion; 5, stopper slot; 6, handle; 7, clamping part; 8, groove; 9, lid; 10, base; 11, slot; 12, chuck; 13, protruding; 14, groove; 15, baffle plate; 16, ovallized column chamber.
Embodiment
Below in conjunction with embodiment, utility model content of the present utility model is described in further detail.Should understand, embodiment of the present utility model is unrestricted the utility model for illustration of the utility model only, when not departing from the utility model technological thought, according to ordinary skill knowledge and customary means, the various replacement made and change, all should be included in scope of the present utility model.
As shown in Figure 1, printingout 1 of the present utility model is T-shaped, and the wide part in described printingout 1 upper end is mark zone portion 3, and the narrow part in lower end is sample area portion 4.Original sequence number can be manufactured in advance in 3 points, the mark zone portion of printingout 1, easy to use, avoid fastening one person's story upon another person.
As shown in Figure 2, described printingout folder comprises the clamping part 7 that handle 6 and elastomeric material are made; Described clamping part 7 is provided with the groove 8 for clamping printingout 1 mark zone portion 3.
As shown in Figure 3, be the figure that above-mentioned Fig. 1 and Fig. 2 is combined, expression be by state during printingout folder gripping printingout 1.
As shown in Figure 4, for covering the structural representation of the lid 9 on printingout box 2, be the lid being suitable for embodiment 1,2,3 and 4 shown in figure; In the middle of embodiment 5, because the inside of printingout box is ovallized column chamber, so the buckle part that lid is arranged should be consistent with the xsect in described ovallized column chamber, be also oval.
As mentioned above, the experiment equipment mentioned in the middle of following examples is.
Embodiment 1
For the experimental provision of the sampling of biological in-situ printingout method and trace dyeing, comprise printingout 1, press from both sides for the printingout of gripping printingout 1, the printingout box 2 for holding printingout 1 of one end open; Described printingout box 2 inwall is provided with the stopper slot 5 in the mark zone portion 3 of restriction printingout 1.
Described lid 9 is provided separately with printingout box.
As shown in Figure 5, Figure 6, the bottom of described printingout box 2 is provided with chuck 12, and described base 10 is provided with the card slot 11 that is used in conjunction with each other with described printingout box 2 and jointly forms the structure piling up printingout box 2 with the chuck 12 of printingout box 2.
As shown in Figure 7, when dyeing or dewater or preserve, fold up in printingout box 2 by printingout 1 printingout, the mark zone portion 3 of printingout 1 is limited in stopper slot 5.
As shown in Figure 8, multiple printingout box 2 is piled up with on base 10, be convenient to management.
Embodiment 2
The difference of the present embodiment and embodiment 1 is:
As shown in Figure 9, Figure 10, lid 9 is fixedly arranged on the opening part of printingout box 2; The bottom of described printingout box 2 is provided with slot 11, and described base 10 is provided with the chuck 12 that is used in conjunction with each other with described printingout box 2 and jointly forms the structure piling up printingout box 2 with the slot 11 of printingout box 2.
As shown in figure 11, multiple printingout boxes 2 of the present embodiment are piled up on base 10.
Embodiment 3
The difference of the present embodiment and embodiment 1 is:
As shown in Figure 12 and Figure 13, the bottom of described printingout box 2 is provided with protruding 13, and described base 10 is provided with the groove 14 that is used in conjunction with each other with described printingout box 2 and jointly forms the structure piling up printingout box 2 with the projection 13 of printingout box 2.Described base 10 is provided with the baffle plate 15 preventing printingout box 2 from rotating for axle with described structure.
Embodiment 4
The difference of the present embodiment and embodiment 1 is:
As shown in Figure 14, Figure 15, the bottom of described printingout box 2 is provided with groove 14, and described base 10 is provided with the projection 13 that is used in conjunction with each other with described printingout box 2 and jointly forms the structure piling up printingout box 2 with the groove 14 of printingout box 2.Described base 10 is provided with the baffle plate 15 preventing printingout box 2 from rotating for axle with described structure.
As shown in figure 16, multiple printingout boxes 2 of the present embodiment are piled up on base 10.
Embodiment 5
The difference of the present embodiment and embodiment 1,2,3 and 4 is:
As shown in figure 17, described printingout box 2 inside is disciform post chamber 16, and described disciform post chamber 16 constitutes the stopper slot 5 in the mark zone portion 3 of described restriction printingout 1 at the two ends of the major axis of its oval cross section.
Embodiment 6
The experiment equipment of above-described embodiment is used to carry out experimental implementation.
1. pair tulase L-J culture adopts Conventional slide method (Figure 18) and original position printingout method sampling (Figure 19) to carry out acid-fast stain microscopic analysis, contrast experiment's effect respectively.
2. couple lunger's Sputum L-J cultivates 1 week culture and carries out original position printingout method sampling acid-fast stain and carry out microscopic analysis (Figure 20,21).
3. operation steps:
1) possess bio-safety require in laboratory and under aseptic technique, press from both sides 2 with printingout 1 as shown in Figure 3 with printingout and printingout 1 is affixed on culture surface, take off gently after leaving standstill the several seconds and open, make bacterium be imprinted on the sample area portion 4 of printingout 1.
2) fix at once after printingout.
3) printingout 1 is placed in dyeing in printingout box 2, washing and drying.Then microscopic analysis is treated in the preservation that closes the lid.
4) during microscopic analysis, the printingout in printingout box 2 is taken out, be fixed on microslide with liquid mounting glue.
5) printingout microscopy, analyzed after put into printingout box preserve.
6) printingout folder can need and custom according to the experiment of operator, before dyeing or after dyeing, parallel promotion is taken off.
As shown in figure 18, for Conventional slide method, namely to mix with physiological saline with oese picking colony on nutrient culture media and spread upon the acid-fast stain optical microscope figure that the tulase L-J method that microslide samples cultivates 4 weeks bacteriums, visible bacterium initial growth state is completely destroyed, major part paramophia is unclear, and acid-fast stain is positive.
As shown in figure 19, for original position printingout method sampling tulase L-J method cultivate 4 weeks bacterium (with the same sputum specimen of Figure 18, parallel inoculated and cultured) acid-fast stain optical microscope figure, the initial growth state of its bacterium is complete clear, the acid-fast stain such as filamentous and huge granule is positive, and can be observed the relation between different shape.
Because tulase L-cell membrane lacks, conventional method is easy to destroy its bacterial structure.Therefore in many research in the past and clinical examination, almost can not obtain the intact form of tulase L-type (comprising other bacterium).And complete clearly bacterium and growth conditions thereof can be obtained by the utility model.
As shown in figure 20, the phlegm L-J method sampled for original position printingout method cultivates tulase (when naked eyes have no bacterium colony) the acid-fast stain optical microscope figure of 1 week.Its bacterium acid-fast stain is positive, and the virgin state of tulase branch growth is high-visible.
As shown in figure 21, the phlegm L-J method sampled for original position printingout method cultivates L-type tulase (when naked eyes have no bacterium colony) the acid-fast stain optical microscope figure of 1 week.Its L-type bacterium acid-fast stain is positive, and due to L-type mycobacterial cell wall disappearance, its thalline is the unlimited extension state that expands, and has cytoplasm to shift out and trend and vestige; As shown in figure 22, the special result such as bud trace can be seen simultaneously.Its ne ar feature is the filamentous modes of reproduction of L-type bacterium.
The utility model device can print gets sickened body or histological section pathology thing, observes the relation between microorganism and body cell.Its original position printingout sampling cell/bacterium, needs further cultivation observational study, can grafting carry out in other cell culture apparatus systems.The utility model device can also be used for in-situ sampling and specific stain (as the histochemical stain etc.) analysis of various microorganism, can also be that molecular immune is as experimental provisions such as immuning hybridizations.Its device can according to specific experiment Demand Design different size.
Claims (10)
1. for the experimental provision of the sampling of biological in-situ printingout method and trace dyeing, it is characterized in that: comprise printingout (1), press from both sides for the printingout of gripping printingout (1), the printingout box (2) for holding printingout (1) of one end open; Described printingout (1) is T-shaped, and the wide part in described printingout (1) upper end is mark zone portion (3), and the narrow part in lower end is sample area portion (4); Described printingout box (2) inwall is provided with the stopper slot (5) in the mark zone portion (3) of restriction printingout (1).
2. the experimental provision for the sampling of biological in-situ printingout method and trace dyeing according to claim 1, is characterized in that: described printingout folder comprises the clamping part (7) that handle (6) and elastomeric material are made; Described clamping part (7) is provided with the groove (8) for clamping printingout (1) mark zone portion (3).
3. the experimental provision dyeed for the sampling of biological in-situ printingout method and trace according to claim 1, is characterized in that: described printingout folder is tweezers.
4. the experimental provision dyeed for the sampling of biological in-situ printingout method and trace according to claim 1, is characterized in that: described printingout box (2) also comprises the lid (9) for covering printingout box (2).
5. the experimental provision dyeed for the sampling of biological in-situ printingout method and trace according to claim 4, is characterized in that: described lid (9) is connected on printingout box (2).
6. the experimental provision dyeed for the sampling of biological in-situ printingout method and trace according to claim 1, it is characterized in that: described printingout box (2) inside is ovallized column chamber (16), and described ovallized column chamber (16) constitutes the stopper slot (5) in the mark zone portion (3) of described restriction printingout (1) at the two ends of the major axis of its oval cross section.
7. the experimental provision dyeed for the sampling of biological in-situ printingout method and trace according to any one of claim 1 ~ 6, it is characterized in that: also comprise the base for piling up printingout box (2), described base (10) is provided with the bottom of printingout box (2) structure be used in conjunction with each other.
8. the experimental provision dyeed for the sampling of biological in-situ printingout method and trace according to claim 7, it is characterized in that: the bottom of described printingout box (2) is provided with slot (11) or chuck (12), described base (10) is provided with the chuck (12) or slot (11) that are used in conjunction with each other with described printingout box (2) and jointly forms the structure piling up printingout box (2) with the slot (11) of printingout box (2) or chuck (12).
9. the experimental provision dyeed for the sampling of biological in-situ printingout method and trace according to claim 7, it is characterized in that: the bottom of described printingout box (2) is provided with projection (13) or groove (14), described base (10) is provided with the groove (14) that is used in conjunction with each other with described printingout box (2) or protruding (13) and jointly forms the structure piling up printingout box (2) with the projection (13) of printingout box (2) or groove (14).
10. the experimental provision dyeed for the sampling of biological in-situ printingout method and trace according to claim 9, is characterized in that: described base (10) is provided with the baffle plate (15) preventing printingout box (2) from rotating for axle with described structure.
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| CN201420676756.3U CN204177654U (en) | 2014-11-13 | 2014-11-13 | For the experimental provision that the sampling of biological in-situ printingout method and trace are dyeed |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109057649A (en) * | 2018-09-25 | 2018-12-21 | 阜阳市祥宇木业有限公司 | A kind of door-plate with active sketching charcoal |
| CN109162596A (en) * | 2018-09-25 | 2019-01-08 | 阜阳市祥宇木业有限公司 | A kind of fixation device of door-plate activity sketching charcoal |
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- 2014-11-13 CN CN201420676756.3U patent/CN204177654U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109057649A (en) * | 2018-09-25 | 2018-12-21 | 阜阳市祥宇木业有限公司 | A kind of door-plate with active sketching charcoal |
| CN109162596A (en) * | 2018-09-25 | 2019-01-08 | 阜阳市祥宇木业有限公司 | A kind of fixation device of door-plate activity sketching charcoal |
| CN109057649B (en) * | 2018-09-25 | 2023-12-29 | 阜阳市祥宇木业有限公司 | Door plant with active carbon strip |
| CN109162596B (en) * | 2018-09-25 | 2024-09-03 | 浙江鸿集建筑科技有限公司 | Fixing device of door plant active carbon strip |
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