CN104970002A - Marine fish plasticizing specimen production method - Google Patents
Marine fish plasticizing specimen production method Download PDFInfo
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- CN104970002A CN104970002A CN201510401464.8A CN201510401464A CN104970002A CN 104970002 A CN104970002 A CN 104970002A CN 201510401464 A CN201510401464 A CN 201510401464A CN 104970002 A CN104970002 A CN 104970002A
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Abstract
The present invention discloses a marine fish plasticizing specimen production method. According to the present invention, with the application of the method to produce the specimen, the vacuum drying treatment is performed, such that the specimen dehydration time is shortened, the specimen production period is shortened, and the preparation process is simple and is easy to perform; and the marine fish specimen prepared by using the plasticizing technology has advantages of long storage, long-term exposure, real morphology and the like, and can be widely used in the fields of teaching, scientific researches and collection watching.
Description
Technical field
The present invention relates to a kind of preparation of specimen technology, more particularly, relate to a kind of marine fishes plasticizing Slide processing.
Background technology
Fishes snd, particularly marine fishes sample, for study marine fishes knowledge provides the learning model of three-dimensional Cognitive Spaces and image, not only in teaching and scientific research, have very important effect, and in column at sample, show and also have important value in artistic ornamental etc.
Biological plasticized technology be a kind of can must special technique as live body tissue preserration, it is by a kind of vacuum process, with silicon rubber, epoxy resin isoreactivity macromolecule polymer, biological sample is permeated, the methods such as gas, light or heating are adopted to be polymerized by polymer in sample again, finally obtained sample.
Polyethylene glycol is utilized to prepare the method for plasticizing sample namely by adopting macromolecular water-soluble plastics--the moisture contained in sample replaced by polyethylene glycol, makes plasticizing sample.Polyethylene glycol is a kind of gentle, non-irritating macromolecular compound of low toxicity, can be completely water-soluble, has good stability.The sample using this plasticizing method to make, has the following advantages: the color of (1) plasticizing sample is slightly deepened than immersed specimen, and shrinkage is not obvious, easily obtains good attitude; (2) soak step by step with the polyethylene glycol of different molecular weight and have anticorrosion fixing effect, made sample is without the need to preservative process; (3) make sample not easily to go mouldy and damage by worms, tasteless without damaged by rats.This technology Problems existing has: (1) experimental implementation is complicated, and the processing time is longer; (2) experiment condition is wayward, by multifactor restriction.In whole biological plasticized preparation of specimen process, dehydration is a very crucial step in whole process, and dehydration not exclusively can affect dipping and the solidification effect of later stage glue, also can cause the mouldy corruption of sample.The problems referred to above limit its application in preparation of specimen.
For these reasons and prior art, the present invention is used for the preparation method of fish plasticizing sample to polyethylene glycol, and particularly the method for marine fishes plasticizing preparation of specimen is improved, and low-temperature vacuum dehydration method is applied to biological plasticized preparation of specimen process.
Summary of the invention
In order to improve the degree of dehydration in marine fishes plasticizing preparation of specimen process, shorten the dewatering time of marine fishes preparation of specimen process, the invention provides the preparation method of the biological plasticized sample of a kind of marine fishes, preparation method is as follows:
(1) choose the marine fishes of skin zero damage, length is no more than 50 centimetres, is highly no more than 20 centimetres, and width is no more than 10 centimetres, and surfacing is clean, puts in a reservoir after rinsing with clear water;
(2) sample is put into vacuum dehydrating at lower temperature case, keep the air pressure in vacuum storehouse to be normal atmosphere (An), snap frozen sample is to-20 DEG C;
(3) after sample fully charge in storehouse, closing valve, opens vacuum plant, by the internal drop of vacuum storehouse to below 600Pa;
(4) close refrigerant condenser, trace opens vacuum storehouse intake valve, and the adjusting range of intake valve is pressed in the dynamic equilibrium of below 600Pa to maintain interior storehouse;
(5) reopening refrigerant condenser when temperature in storehouse rises to-5 DEG C makes temperature in storehouse be down to-25 DEG C, repeats this step 2 time;
(6) sample is put into absolute ethyl alcohol and soak 10min;
(7) sample is put into 80% ethanolic solution to soak 20 hours;
(8) sample is put into 20% polyethylene glycol and 1% thymol mixed solution to soak 6 hours;
(9) in sample body, inject 30% polyglycol solution from oral cavity and belly, sample is put into 30% polyglycol solution and soak 6 hours;
(10) sample is put into 50% polyglycol solution to soak 8 hours;
(11) sample is put into 70% polyglycol solution to soak 10 hours;
(12) sample is put into 90% polyglycol solution to soak 15 hours;
(13) with absolute ethyl alcohol and 1% thymol wiping sample surface, and remove the polyglycol solution of excess surface, put into 30%PVP solution and soak 10min;
(14) take out sample to place 2 days under the low light level, in dark dry place, nature dries;
(15) sample is immersed 10rin in 30%PVP solution, until sample is moistening, the full PVP solution of eye leaching that sample dewaters shrivelled, takes out dry, repeats this step 3 time.
Described sample is cod.
Described sample is extra large Nian.
Described step (10) is soaked 8 hours for sample being put into 60% polyglycol solution.
Described step (11) is soaked 10 hours for sample being put into 80% polyglycol solution.
Described step (12) is soaked 15 hours for sample being put into 100% polyglycol solution.
The invention has the beneficial effects as follows: (1) substantially reduces the time of dehydration in the manufacturing process of the biological plasticized sample of marine fishes, saved the time of preparation of specimen; (2) in the manufacturing process of the biological plasticized sample of marine fishes, use vacuum dehydration, ensure that the completeness of dehydration, avoid dipping and solidification effect that dehydration not exclusively affects later stage glue.
Embodiment
(1) choose the cod of skin zero damage, length is no more than 50 centimetres, is highly no more than 20 centimetres, and width is no more than 10 centimetres, and surfacing is clean, puts in a reservoir after rinsing with clear water;
(2) cod sample is put into vacuum dehydrating at lower temperature case, keep the air pressure in vacuum storehouse to be normal atmosphere (An), snap frozen sample is to-20 DEG C;
(3) after cod sample fully charge in storehouse, closing valve, opens vacuum plant, by the internal drop of vacuum storehouse to below 600Pa;
(4) close refrigerant condenser, trace opens vacuum storehouse intake valve, and the adjusting range of intake valve is pressed in the dynamic equilibrium of below 600Pa to maintain interior storehouse;
(5) reopening refrigerant condenser when temperature in storehouse rises to-5 DEG C makes temperature in storehouse be down to-25 DEG C, repeats this step 2 time;
(6) in above-mentioned vacuum storehouse, take out cod sample, cod sample is put into absolute ethyl alcohol and soaks 10min;
(7) cod sample is put into 80% ethanolic solution to soak 20 hours;
(8) cod sample is put into 20% polyethylene glycol and 1% thymol mixed solution to soak 6 hours;
(9) in cod sample body, inject 30% polyglycol solution from oral cavity and belly, sample is put into 30% polyglycol solution and soak 6 hours;
(10) cod sample is put into 50% polyglycol solution to soak 8 hours;
(11) cod sample is put into 70% polyglycol solution to soak 10 hours;
(12) cod sample is put into 90% polyglycol solution to soak 15 hours;
(13) with absolute ethyl alcohol and 1% thymol wiping sample surface, and remove the polyglycol solution of excess surface, put into 30%PVP solution and soak 10min;
(14) take out cod sample to place 2 days under the low light level, in dark dry place, nature dries;
(15) cod sample is immersed 10min in 30%PVP solution, until sample is moistening, the full PVP solution of eye leaching that sample dewaters shrivelled, takes out dry, repeats this step 3 time.
The above; be only the present invention's preferably embodiment; but protection scope of the present invention is not limited thereto; anyly be familiar with those skilled in the art in the technical scope that the present invention discloses; be equal to according to technical scheme of the present invention and inventive concept thereof and replace or change, all should be encompassed within protection scope of the present invention.
Claims (6)
1. a preparation method for marine fishes plasticizing sample, it is characterized in that, preparation of specimen's step is as follows:
(1) choose the marine fishes of skin zero damage, length is no more than 50 centimetres, is highly no more than 20 centimetres, and width is no more than 10 centimetres, and surfacing is clean, puts in a reservoir after rinsing with clear water;
(2) sample is put into vacuum dehydrating at lower temperature case, keep the air pressure in vacuum storehouse to be normal atmosphere (An), snap frozen sample is to-20 DEG C;
(3) after sample fully charge in storehouse, closing valve, opens vacuum plant, by the internal drop of vacuum storehouse to below 600Pa;
(4) close refrigerant condenser, trace opens vacuum storehouse intake valve, and the adjusting range of intake valve is pressed in the dynamic equilibrium of below 600Pa to maintain interior storehouse;
(5) reopening refrigerant condenser when temperature in storehouse rises to-5 DEG C makes temperature in storehouse be down to-25 DEG C, repeats this step 2 time;
(6) sample is put into absolute ethyl alcohol and soak 10min;
(7) sample is put into 80% ethanolic solution to soak 20 hours;
(8) sample is put into 20% polyethylene glycol and 1% thymol mixed solution to soak 6 hours;
(9) in sample body, inject 30% polyglycol solution from oral cavity and belly, sample is put into 30% polyglycol solution and soak 6 hours;
(10) sample is put into 50% polyglycol solution to soak 8 hours;
(11) sample is put into 70% polyglycol solution to soak 10 hours;
(12) sample is put into 90% polyglycol solution to soak 15 hours;
(13) with absolute ethyl alcohol and 1% thymol wiping sample surface, and remove the polyglycol solution of excess surface, put into 30%PVP solution and soak 10min;
(14) take out sample to place 2 days under the low light level, in dark dry place, nature dries;
(15) sample is immersed 10min in 30%PVP solution, until sample is moistening, the full PVP solution of eye leaching that sample dewaters shrivelled, takes out dry, repeats this step 3 time.
2. the preparation method of a kind of marine fishes plasticizing sample according to claim 1, it is characterized in that, described sample is cod.
3. the preparation method of a kind of marine fishes plasticizing sample according to claim 1, it is characterized in that, described sample is extra large Nian.
4. the preparation method of a kind of marine fishes plasticizing sample according to claim 1, it is characterized in that, described step (10) is soaked 8 hours for sample being put into 60% polyglycol solution.
5. the preparation method of a kind of marine fishes plasticizing sample according to claim 1, it is characterized in that, described step (11) is soaked 10 hours for sample being put into 80% polyglycol solution.
6. the preparation method of a kind of marine fishes plasticizing sample according to claim 1, it is characterized in that, described step (12) is soaked 15 hours for sample being put into 100% polyglycol solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510401464.8A CN104970002A (en) | 2015-07-09 | 2015-07-09 | Marine fish plasticizing specimen production method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510401464.8A CN104970002A (en) | 2015-07-09 | 2015-07-09 | Marine fish plasticizing specimen production method |
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| CN104970002A true CN104970002A (en) | 2015-10-14 |
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| CN201510401464.8A Pending CN104970002A (en) | 2015-07-09 | 2015-07-09 | Marine fish plasticizing specimen production method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112824864A (en) * | 2019-11-20 | 2021-05-21 | 中国农业大学 | Plasticizing method of traditional Chinese medicine decoction piece specimen |
| CN113180033A (en) * | 2021-04-28 | 2021-07-30 | 黄倞朗 | Preparation method of small biological specimen |
-
2015
- 2015-07-09 CN CN201510401464.8A patent/CN104970002A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112824864A (en) * | 2019-11-20 | 2021-05-21 | 中国农业大学 | Plasticizing method of traditional Chinese medicine decoction piece specimen |
| CN113180033A (en) * | 2021-04-28 | 2021-07-30 | 黄倞朗 | Preparation method of small biological specimen |
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Application publication date: 20151014 |
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| WD01 | Invention patent application deemed withdrawn after publication |