CN104041482A - Manufacturing method of lepidopteron plastinated specimens - Google Patents
Manufacturing method of lepidopteron plastinated specimens Download PDFInfo
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- CN104041482A CN104041482A CN201410178549.XA CN201410178549A CN104041482A CN 104041482 A CN104041482 A CN 104041482A CN 201410178549 A CN201410178549 A CN 201410178549A CN 104041482 A CN104041482 A CN 104041482A
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Abstract
The invention discloses a manufacturing technology of lepidopteron plastinated specimens. By the method for manufacturing specimens, instruments and equipment used are simple. A constant temperature incubator is only needed for a simple drying treatment, and vacuum drying and pressure infiltration processing are not required. Plastification time is shortened. The manufacturing process is simpler and easier to do. Reagents which are harmful to human body are not used during the manufacturing process. Manufactured specimens can directly be contacted. External morphology, especially scale, is not influenced. Manufactured specimens can be widely applied in teaching, scientific research and collection and display. According to plastinated specimens of butterflies (such as papilionoidea, brush-footed butterflies and Pieridae), phalaenae (such as tussah and silkworm) and the like which are manufactured by the above technology, scale is maintained perfect, forms are lifelike, and the color of larva is very similar to that of a living body.
Description
Technical field
The present invention relates to a kind of preparation of specimen technology, more particularly, relate to a kind of lepidopterous insects plasticizing Slide processing.
Background technology
The method of preparing at present lepidopterous insects sample mainly contains the method for spreading the wings and artificial amber technology.
Suitable spreading board, be soft condition before lepidopterous insects health is not dry time, is selected by the preparation of specimen of spreading the wings, and with tweezers gripping insect, is placed on spreading board, selects suitable insect needle or pin, vertically inserts from the center of insect mesothorax or metathorax.Be pressed on wing with long smooth transparent soft paper slip, nail on pin, dipteron is fixed up, after finish-drying, take out and be stored in for a long time in Riker mount.The advantage of sample of spreading the wings be manufacture simple, preserve without other solution or container, but the sample of spreading the wings is due to the easy contraction distortion of dehydration, and easily damages by worms and go mouldy, surface scale in manufacturing process or easily damaged in the time using thereafter, has had a strong impact on the complete of insect external form.Sample insect is had relatively high expectations simultaneously, must in the time that health is soft condition, make.Made sample is difficult for preservation, need be placed in Riker mount dustproof, moistureproof, sealing, and require to put into pest repellant and desiccant, will prevent strenuous vibration, otherwise easily breakage occurs while moving, and sunlight direct irradiation can cause polypide change color.
Technology source:
1. Su Shao section.The spread the wings improvement [J] of shaping technology of lepidopterous insects.Experimental teaching and instrument, 2007, (7): 101.
2. Nie passes friend, Yang Peng.A kind of simple making method [J] of lepidopterous insects wing sample.Fuyang Teachers College's journal (natural science edition), 2006,23 (4): 87-88.
Artificial amber is to form principle according to natural amber, adopts the method for synthetic resin specimen embedding and makes.Manufacturing process is roughly divided into following steps: urea aldehyde monomer processed, synthetic resin, water base plate, specimen embedding, lid top panel, arrangement, preservation sample.The amber sample that this method makes, sparkling and crystal-clear bright, life-like.General only edge slightly need work modified arrangement, and the sanding and polishing effect of having ready conditions is better.But in preparation of specimen's process, will use harmful reagent as formaldehyde etc., sample cannot directly contact, and is difficult for observing, and sample is difficult for preserving, and the amber of making will be placed in special Riker mount and preserve, and also will avoid contacting strong acid, highly basic when preservation, can not weight, keep air dry.
Technology source:
1. have protect and.The easy making [J] of artificial amber sample.Biology circular, 1996,31 (1): 38.
2. road virtue, Ye Xiangku, the happy handle of the Big Dipper of well.The manufacturing experimently again of artificial amber sample [J].Land-reclaimable Journal of Teachers College, 1997,1:94-95.
3. Luo Xiang dragon.Artificial amber technology [J].Technology and market, 2001,24:24.
4. Yao Zhi light.Macromolecular material embedding type casting moulding technical study [J].Mongolia's petrochemical industry, 2011,17:6-7.
Summary of the invention
The invention provides a kind of lepidopterous insects plasticizing Slide processing, to reach the making flow process of simplifying lepidopterous insects plasticizing sample, reduce technical requirement, shorten the effect of fusion time, make preparation process more simple.Concrete summary of the invention is as follows:
(1) larva sample preparation:
(1) choose of the right age larva, snap frozen is put to death;
(2) guarantor's look is heated to 80 DEG C of thawings with reagent, while being cooled to 70-75 DEG C, polypide is put into solution and soak 20 minutes, then with distilled water by clean polypide surface clean;
(3) polypide is placed in to 50% molecular weight 400 polyethylene glycol and soaks two hours, stir polypide therebetween every half an hour, keep polypide surface uniform contact molecular weight 400 polyethylene glycol, after taking out, use blotting paper wiped clean;
(4) polyethylene glycol is filled and is heated to melt completely with reagent, moistening syringe, draws a small amount of filling reagent with syringe and is injected into full state from polypide caudal horn;
(5) polypide is placed in to sealing solution and soaks about 10 minutes, take out nature and dry.
Described guarantor's look agent prescription is: 50mL molecular weight 400 polyethylene glycol, 50mL distilled water, 2.6g tartaric acid and 1g anhydrous cupric sulfate; Various materials are mixed, at normal temperatures and pressures, be heated to dissolve completely.
Described filling agent prescription is: 40g molecular weight 10000 polyethylene glycol, 180mL molecular weight 600 polyethylene glycol and 20mL distilled water; Various materials are mixed, at normal temperatures and pressures, be heated to dissolve completely.
Described sealing solution formula is: 10g PVP K-30,1g potassium hydroxide, 5mL 95% ethanol and 95mL distilled water; Various materials are mixed, at normal temperatures and pressures, be heated to dissolve completely.
(2) preparation of adult sample (taking moth class as example):
(1) choose the adult of complete form, snap frozen is put to death or etherization is put to death;
(2) by the insect polypide preparation method of spreading the wings, polypide is placed on spreading board, stirring fore wing with dissecting needle, to make it trailing edge vertical with polypide, and proloma is close to fore wing trailing edge, shaping, after finish-drying, the plasticizing reagent that is placed in 30 DEG C soaks ten minutes, the polypide of constantly overturning therebetween, soaks into completely to polypide inside plasticizing reagent;
(3) polypide dried or dry, then dipping in warm water with cotton balls residual polyethylene glycol is cleaned out;
(4) after polypide bone dry, at polypide surface spraying sealing reagent, dry.
Described plasticizing agent prescription is: 10g molecular weight 10000 polyethylene glycol, 30mL molecular weight 1000 polyethylene glycol, 70mL molecular weight 600 polyethylene glycol and 50mL distilled water; Various materials are mixed, at normal temperatures and pressures, be heated to dissolve completely.
Described sealing reagent is: 20g PVP K-30,1g potassium hydroxide and 30mL distilled water; Various materials are mixed, at normal temperatures and pressures, be heated to dissolve completely.
Core technology: polyethylene glycol plays the effect that makes protein denaturation stable protein, for main anticorrosion, dehydrated reagent, can avoid that sample rots, rotten, shrinkage with polyethylene glycol.And PVP K-30 is used the main component of reagent as sealing, form fine and close isolation layer on sample surface, there is the effect of isolated air, anti-oxidation, mildew resistant moistureproof, make the holding time of sample longer.Be expelled in sample body with reagent as filling taking the polyethylene glycol of variable concentrations, can maintain the form of sample.In whole plasticizing process, to note the assurance time, the especially time of dehydration, overlong time, sample is shrinkage too, is difficult for carrying out next step processing; Time is too short, in the residual body of excess moisture, causes sample perishable.The key of plasticizing process is to make sample even with contacting of reagent with plasticizing, requires the variation of moment concern sample in plasticizing process.While finally processing by lock solution, sample will dry, last In Shade preservation.
The beneficial effect of the invention:
1. use polyethylene glycol instead of formaldehyde as preservative, reduced the injury that human body is caused; Polyethylene glycol, as dehydrated reagent, operates more easy;
2. copper sulphate and tartaric acid are configured to the guarantor look reagent of proportionate solution as larva, kept the natural colored of larva;
3. adult surface, through sealing solution-treated, can avoid scale to come off, and the sample of simultaneously making can directly touch;
4. for to make the form of animal truer, adopted plasticizing to fill in its body with reagent, made its form more true to nature.
Embodiment
now further illustrate the present invention in conjunction with embodiment.
(1) larva sample preparation:
(1) choose of the right age larva, snap frozen is put to death;
(2) guarantor's look is heated to 80 DEG C of thawings with reagent, while being cooled to 70-75 DEG C, polypide is put into solution and soak 20 minutes, then with distilled water by clean polypide surface clean;
(3) polypide is placed in to 50% molecular weight 400 polyethylene glycol and soaks two hours, stir polypide therebetween every half an hour, keep polypide surface uniform contact molecular weight 400 polyethylene glycol, after taking out, use blotting paper wiped clean;
(4) polyethylene glycol is filled and is heated to melt completely with reagent, moistening syringe, draws a small amount of filling reagent with syringe and is injected into full state from polypide caudal horn;
(5) polypide is placed in to sealing solution and soaks about 10 minutes, take out nature and dry.
Described guarantor's look agent prescription is: 50mL molecular weight 400 polyethylene glycol, 50mL distilled water, 2.6g tartaric acid and 1g anhydrous cupric sulfate; Various materials are mixed, at normal temperatures and pressures, be heated to dissolve completely.
Described filling agent prescription is: 40g molecular weight 10000 polyethylene glycol, 180mL molecular weight 600 polyethylene glycol and 20mL distilled water; Various materials are mixed, at normal temperatures and pressures, be heated to dissolve completely.
Described sealing solution formula is: 10g PVP K-30,1g potassium hydroxide, 5mL 95% ethanol and 95mL distilled water; Various materials are mixed, at normal temperatures and pressures, be heated to dissolve completely.
(2) preparation of adult sample (taking moth class as example):
(1) choose the adult of complete form, snap frozen is put to death or etherization is put to death;
(2) by the insect polypide preparation method of spreading the wings, polypide is placed on spreading board, stirring fore wing with dissecting needle, to make it trailing edge vertical with polypide, and proloma is close to fore wing trailing edge, shaping, after finish-drying, the plasticizing reagent that is placed in 30 DEG C soaks ten minutes, the polypide of constantly overturning therebetween, soaks into completely to polypide inside plasticizing reagent;
(3) polypide dried or dry, then dipping in warm water with cotton balls residual polyethylene glycol is cleaned out;
(4) after polypide bone dry, at polypide surface spraying sealing reagent, dry.
Described plasticizing agent prescription is: 10g molecular weight 10000 polyethylene glycol, 30mL molecular weight 1000 polyethylene glycol, 70mL molecular weight 600 polyethylene glycol and 50mL distilled water; Various materials are mixed, at normal temperatures and pressures, be heated to dissolve completely.
Described sealing agent prescription is: 20g PVP K-30,1g potassium hydroxide and 30mL distilled water; Various materials are mixed, at normal temperatures and pressures, be heated to dissolve completely.
The purposes of lepidopterous insects plasticizing sample:
1. carry out insect specimen creative activity in open laboratory, can improve student's manipulative ability;
2. the function of insect specimen in teaching, is that it can make teaching more directly perceived and vivid, enlivens the classroom atmosphere of zoology teaching simultaneously, increases the entomological interest of Students ' Learning;
3. play in daily life decoration function, owing to preserving for a long time, not fugitive color distortion, nontoxic, so there is good ornamental value;
4. can be for scientific research, for researcher is provided convenience.Reduced the injury to health simultaneously.
The above; it is only preferably embodiment of the present invention; but protection scope of the present invention is not limited to this; any be familiar with those skilled in the art the present invention disclose technical scope in; be equal to replacement or changed according to technical scheme of the present invention and inventive concept thereof, within all should being encompassed in protection scope of the present invention.
Claims (7)
1. lepidopterous insects plasticizing Slide processing, is characterized in that, described lepidopterous insects is larva, and making step is as follows:
(1) choose of the right age larva, snap frozen is put to death;
(2) guarantor's look of preparation is heated to 80 DEG C with reagent, while being cooled to 70-75 DEG C, polypide is placed in to solution and soaks 20 minutes, then with distilled water by clean polypide surface clean;
(3) polyethylene glycol that polypide is placed in to 50% molecular weight 400 soaks two hours, stirs polypide therebetween every half an hour, keeps polypide surface uniform contact polyethylene glycol, after taking out, uses blotting paper wiped clean;
(4) filling configuring is heated to dissolve completely with reagent, moistening syringe, draws a small amount of filling reagent with syringe and is injected into full state from polypide caudal horn;
(5) polypide is put into the sealing solution preparing and soak about 10 minutes, take out nature and dry.
2. lepidopterous insects plasticizing Slide processing according to claim 1, is characterized in that, described guarantor's look reagent is the polyethylene glycol by 50mL molecular weight 400,50mL distilled water, 2.6g tartaric acid and 1g anhydrous cupric sulfate, at normal temperatures and pressures, be mixed and heated to completely and dissolve and make.
3. lepidopterous insects plasticizing Slide processing according to claim 1, it is characterized in that, described filling reagent is by 40g molecular weight 10000 polyethylene glycol, 180mL molecular weight 600 polyethylene glycol and 20mL distilled water, at normal temperatures and pressures, be mixed and heated to completely and dissolve and make.
4. lepidopterous insects plasticizing Slide processing according to claim 1, is characterized in that, described sealing solution is by 10g PVP K-30,1g potassium hydroxide, 5mL 95% ethanol and 95mL distilled water, at normal temperatures and pressures, be mixed and heated to completely and dissolve and make.
5. lepidopterous insects plasticizing Slide processing, is characterized in that, described lepidopterous insects is adult, and making step is as follows:
(1) choose the adult of complete form, snap frozen is put to death or etherization is put to death;
(2) by the insect Slide processing of spreading the wings, polypide is placed on spreading board, stirring fore wing with dissecting needle, to make it trailing edge vertical with polypide, and proloma is close to fore wing trailing edge, shaping, after finish-drying, the plasticizing reagent that is placed in 30 DEG C soaks ten minutes, the polypide of constantly overturning therebetween, soaks into completely to polypide inside plasticizing reagent;
(3) polypide dried or dry, then dipping in warm water with cotton balls residual polyethylene glycol is cleaned out;
(4) after polypide bone dry, at polypide surface spraying sealing reagent, dry.
6. lepidopterous insects plasticizing Slide processing according to claim 5, it is characterized in that, described plasticizing reagent is by 10g molecular weight 10000 polyethylene glycol, 30mL molecular weight 1000 polyethylene glycol, 70mL molecular weight 600 polyethylene glycol and 50mL distilled water, at normal temperatures and pressures, be mixed and heated to completely and dissolve and make.
7. lepidopterous insects plasticizing Slide processing according to claim 5, it is characterized in that, described sealing reagent is by 20g PVP K-30,1g potassium hydroxide and 30mL distilled water, at normal temperatures and pressures, be mixed and heated to completely and dissolve and make.
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| CN201410178549.XA CN104041482B (en) | 2014-04-30 | 2014-04-30 | Manufacturing method of lepidopteron plastinated specimens |
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| CN201410178549.XA CN104041482B (en) | 2014-04-30 | 2014-04-30 | Manufacturing method of lepidopteron plastinated specimens |
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| CN104041482A true CN104041482A (en) | 2014-09-17 |
| CN104041482B CN104041482B (en) | 2015-05-20 |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105427728A (en) * | 2016-01-19 | 2016-03-23 | 金陵科技学院 | Method for removing wing surface scales of lepidopterous insects |
| CN108207928A (en) * | 2017-12-29 | 2018-06-29 | 湖北工程学院 | Insect specimen desquamation agent, preparation method and insect specimen scale removal methods |
| CN108244093A (en) * | 2018-04-10 | 2018-07-06 | 大连理工大学 | A kind of insect specimen color preserving agent, preparation method and color-retention method |
| CN109166439A (en) * | 2018-10-30 | 2019-01-08 | 河南科技大学 | A kind of production method of the stomach section silica gel model of sheep |
| CN110839611A (en) * | 2019-11-29 | 2020-02-28 | 福建农林大学 | Method for preparing vermiform insect larva specimen and application thereof |
| CN111213632A (en) * | 2019-11-19 | 2020-06-02 | 长春中医药大学 | Method and equipment for making animal medicine epoxy resin specimen |
| CN111480643A (en) * | 2020-06-01 | 2020-08-04 | 中国科学院昆明动物研究所 | Insect specimen manufacturing method |
| CN113016774A (en) * | 2021-03-31 | 2021-06-25 | 杭州颖珍贸易有限公司 | Equipment of plastify animal specimen |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105427728A (en) * | 2016-01-19 | 2016-03-23 | 金陵科技学院 | Method for removing wing surface scales of lepidopterous insects |
| CN108207928A (en) * | 2017-12-29 | 2018-06-29 | 湖北工程学院 | Insect specimen desquamation agent, preparation method and insect specimen scale removal methods |
| CN108207928B (en) * | 2017-12-29 | 2020-07-28 | 湖北工程学院 | Scale removing agent for insect specimen, preparation method of scale removing agent and scale removing method for insect specimen |
| CN108244093A (en) * | 2018-04-10 | 2018-07-06 | 大连理工大学 | A kind of insect specimen color preserving agent, preparation method and color-retention method |
| CN109166439A (en) * | 2018-10-30 | 2019-01-08 | 河南科技大学 | A kind of production method of the stomach section silica gel model of sheep |
| CN111213632A (en) * | 2019-11-19 | 2020-06-02 | 长春中医药大学 | Method and equipment for making animal medicine epoxy resin specimen |
| CN110839611A (en) * | 2019-11-29 | 2020-02-28 | 福建农林大学 | Method for preparing vermiform insect larva specimen and application thereof |
| CN111480643A (en) * | 2020-06-01 | 2020-08-04 | 中国科学院昆明动物研究所 | Insect specimen manufacturing method |
| CN113016774A (en) * | 2021-03-31 | 2021-06-25 | 杭州颖珍贸易有限公司 | Equipment of plastify animal specimen |
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| CN104041482B (en) | 2015-05-20 |
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